Publications by authors named "Chenguang Zhang"

71 Publications

GWAS-linked hot loci predict short-term functional outcome and recurrence of ischemic stroke in Chinese population.

Am J Transl Res 2021 15;13(5):4521-4534. Epub 2021 May 15.

Department of Neurology, First Affiliated Hospital of China Medical University Shenyang, Liaoning, China.

In the past decade, an increasing number of genome-wide association studies (GWASs) have been applied to ischemic stroke (IS) susceptibility and recovery. In our study, six GWAS-linked hot loci ( rs10744777, rs2107595, rs532436, rs76221407, rs1842681 and rs2236406) were selected, genotyped and analyzed in 982 IS patients from northern Chinese population, in order to explore their roles in stroke functional outcome and recurrence risk. We found that rs2236406 was significantly associated with functional outcome after stroke. Further logistic regression analysis revealed the variant genotype TC/CC of rs2236406 as an independent prognostic factor for poor stroke recovery in Chinese population. Meanwhile, we observed that GA/AA genotype of rs532436 was statistically correlated with the increased risk of stroke recurrence, especially for patients with large-artery atherosclerosis. Moreover, multivariate Cox analysis identified rs12342 as an independent predictor for IS recurrence. Further functional annotation analysis demonstrated that rs2236406 and rs2043211 were located in the transcriptionally active region, and could change the regulatory motif, transcription factor binding capacity and expression level of RP11-435O5.5 (antisense to PTCH1) and ABO, respectively. In summary, our results suggested that rs2236406 and rs532436 may be novel genetic markers and potential therapeutic targets for stroke prognosis. More studies are required to confirm our findings and clarify the underlying molecular mechanisms.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205681PMC
May 2021

HOXD Antisense Growth-Associated Long Noncoding RNA Promotes Triple-Negative Breast Cancer Progression by Activating Wnt Signaling Pathway.

J Breast Cancer 2021 Jun 28;24(3):315-329. Epub 2021 Apr 28.

Department of Breast Surgery, Zhengxing Hospital, Zhangzhou, China.

Purpose: Triple-negative breast cancer (TNBC) is the most lethal subtype of breast cancer owing to high heterogeneity, aggressive nature, and lack of treatment options, which has a substantial deleterious effect on patients' lives. HOXD antisense growth-associated long noncoding RNA (lncRNA) (HAGLR) plays tumor-promoting roles in many cancers. In this study, we aimed to explore the role of HAGLR in TNBC.

Methods: Quantitative real-time polymerase chain reaction assays were used to examine the expression of RNAs. Functional experiments were conducted to test the biological behavior of TNBC cells. Moreover, MS2-RNA immunoprecipitation, luciferase reporter, and RNA pull-down assays were conducted to verify the binding relationship between HAGLR, microRNA-143-5p (miR-143-5p), and serine- and arginine-rich splicing factor 1 (SRSF1).

Results: HAGLR was found to be highly expressed in TNBC tissues and cells, and inhibiting HAGLR suppressed cell proliferation, migration, and invasion and promoted cell apoptosis in TNBC. Meanwhile, miR-93-5p was shown to bind to HAGLR and SRSF1. In addition, SRSF1 plays an oncogenic role in TNBC. Importantly, HAGLR could activate the Wnt signaling pathway by sponging miR-93-5p to upregulate SRSF1; thus, accelerating TNBC progression.

Conclusion: HAGLR could promote the progression of TNBC through the miR-93-5p/SRSF1 axis to activate the Wnt signaling pathway.
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http://dx.doi.org/10.4048/jbc.2021.24.e24DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250102PMC
June 2021

MET and FASN as Prognostic Biomarkers of Triple Negative Breast Cancer: A Systematic Evidence Landscape of Clinical Study.

Front Oncol 2021 27;11:604801. Epub 2021 May 27.

Hunan University of Medicine, Huaihua, China.

Background: To know the expression of Mesenchymal-Epithelial Transition factor (MET) and Fatty Acid Synthase (FASN) in Triple Negative Breast Cancer (TNBC) patients, as well as its relationship with clinical pathological characteristic and prognosis.

Methods: we used immunohistochemistry staining to detect the expression of MET and FASN for those 218 TNBC patients, and analyze their relationship with the clinical pathological characteristic and prognosis.

Results: 130 and 65 out of 218 TNBC patients were positive for MET in the cancer and adjacent tissues respectively. 142 and 30 out of 218 TNBC patients were positive for FASN in the cancer and adjacent tissues respectively. Positive expression of MET and FASN were significantly correlated with lymph node metastasis, pathological TNM, and pathological Stage. In addition, the positive expression of MET and FASN were correlated with recurrence and metastasis. The combined use of MET and FASN can better predict the survival condition.

Conclusions: Our results indicated that MET and FASN showed good predictive ability for TNBC. Combined use of MET and FASN were recommended in order to make a more accurate prognosis for TNBC.
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http://dx.doi.org/10.3389/fonc.2021.604801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190390PMC
May 2021

Elliptical Pipette Generated Large Microdroplets for POC Visual ddPCR Quantification of Low Viral Load.

Anal Chem 2021 04 16;93(16):6456-6462. Epub 2021 Apr 16.

Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, United States.

Rapid point-of-care (POC) quantification of low virus RNA load would significantly reduce the turn-around time for the PCR test and help contain a fast-spreading epidemic. Herein, we report a droplet digital PCR (ddPCR) platform that can achieve this sensitivity and rapidity without bulky lab-bound equipment. The key technology is a flattened pipette tip with an elliptical cross-section, which extends a high aspect-ratio microfluidic chip design to pipette scale, for rapid (<5 min) generation of several thousand monodispersed droplets ∼150 to 350 μm in size with a CV of ∼2.3%. A block copolymer surfactant (polyoxyalkylene F127) is used to stabilize these large droplets in oil during thermal cycling. At this droplet size and number, positive droplets can be counted by eye or imaged by a smartphone with appropriate illumination/filtering to accurately quantify up to 100 target copies. We demonstrate with 2019 nCoV-PCR assay LODs of 3.8 copies per 20 μL of sample and a dynamic range of 4-100 copies. The ddPCR platform is shown to be inhibitor resistant with spiked saliva samples, suggesting RNA extraction may not be necessary. It represents a rapid 1.5-h POC quantitative PCR test that requires just a pipette equipped with elliptical pipette tip, a commercial portable thermal cycler, a smartphone, and a portable trans-illuminator, without bulky and expensive micropumps and optical detectors that prevent POC application.
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http://dx.doi.org/10.1021/acs.analchem.1c00192DOI Listing
April 2021

Clinical study of combined application of indocyanine green and methylene blue for sentinel lymph node biopsy in breast cancer.

Medicine (Baltimore) 2021 Apr;100(15):e25365

Department of Breast Surgery, The Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.

Objective: This study aims to investigate the feasibility of combined application of indocyanine green (ICG) and methylene blue (MB) for sentinel lymph node biopsy (SLNB) in patients with early breast cancer.

Methods: A total of 415 patients who underwent SLNB and axillary lymph node dissection were enrolled. Sentinel lymph node (SLN) was assessed in 197 patients with ICG and MB combination method, while, the other 218 patients were detected by MB method alone. During surgery, all SLNs were harvested for pathological examination. Then the detection rate and false negative rate of SLNs were comparatively analyzed between the 2 groups.

Results: In the combined ICG and MB group, the detection rate of SLNs was 96.9%, significantly higher than that of MB group, which was 89.7% (P < .05). Similarly, in combined group, the average number of SLNs per patient was 3.0, much higher than that of MB group, which was 2.1 (P < .05). There was no statistically significant difference in false negative rate between combined group and MB alone group, which was 7.3% and 10.5%, respectively (P = .791).

Conclusion: The combined application of ICG and MB for SLNB is much more effective than MB alone in detecting SLNs.
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http://dx.doi.org/10.1097/MD.0000000000025365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051969PMC
April 2021

Assessing effects of reopening policies on COVID-19 pandemic in Texas with a data-driven transmission model.

Infect Dis Model 2021 23;6:461-473. Epub 2021 Feb 23.

Department of Biostatistics and Data Science, The University of Texas Health Science Center at Houston, Houston, USA.

While the Coronavirus Disease 2019 (COVID-19) pandemic continues to threaten public health and safety, every state has strategically reopened the business in the United States. It is urgent to evaluate the effect of reopening policies on the COVID-19 pandemic to help with the decision-making on the control measures and medical resource allocations. In this study, a novel SEIR model was developed to evaluate the effect of reopening policies based on the real-world reported COVID-19 data in Texas. The earlier reported data before the reopening were used to develop the SEIR model; data after the reopening were used for evaluation. The simulation results show that if continuing the "stay-at-home order" without reopening the business, the COVID-19 pandemic could end in December 2020 in Texas. On the other hand, the pandemic could be controlled similarly as the case of no-reopening only if the contact rate was low and additional high magnitude of control measures could be implemented. If the control measures are only slightly enhanced after reopening, it could flatten the curve of the COVID-19 epidemic with reduced numbers of infections and deaths, but it might make the epidemic last longer. Based on the reported data up to July 2020 in Texas, the real-world epidemic pattern is between the cases of the low and high magnitude of control measures with a medium risk of contact rate after reopening. In this case, the pandemic might last until summer 2021 to February 2022 with a total of 4-10 million infected cases and 20,080-58,604 deaths.
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http://dx.doi.org/10.1016/j.idm.2021.02.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901308PMC
February 2021

Genome-Wide Detection of mA-Associated Genetic Polymorphisms Associated with Ischemic Stroke.

J Mol Neurosci 2021 Feb 12. Epub 2021 Feb 12.

Department of Neurology, First Affiliated Hospital of China Medical University, Liaoning, Shenyang, China.

N-Methyladenosine (mA) methylation is the most abundant post-transcription modification in eukaryotes and plays a vital role in many pathological conditions including cerebral ischemia-reperfusion injury and vascular inflammation. Moreover, recent studies have reported that single-nucleotide polymorphisms (SNPs) can affect the mA modification. Therefore, we investigated the relationship between mA-SNPs and ischemic stroke (IS) risk through integrative analysis of an IS genome-wide association study and mA-SNP list from the m6AVar database. Next, we performed eQTL and differential expression analysis to support these IS-associated mA-SNPs. Finally, using the identified polymorphisms, a PPI network was constructed using the STRING database, and GO and pathway enrichment analyses were performed using the DAVID online tool. Accordingly, we identified 305 IS-associated SNPs that could affect mA methylation. Next, 158 of these SNPs were determined to have eQTL signals on local genes. We further identified 84 local genes (containing a total of 87 SNPs) that were differentially expressed in IS patients. Finally, we identified several biological processes and pathways related to IS pathogenesis, such as "leukocyte migration" and "focal adhesion." In summary, our study detected dozens of mA-SNPs as critical functional polymorphisms and novel genetic biomarkers for IS susceptibility and provided a new means of elucidating the biological mechanism underlying IS development.
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http://dx.doi.org/10.1007/s12031-021-01805-xDOI Listing
February 2021

Author Correction: Exosomes secreted by human adipose mesenchymal stem cells promote scarless cutaneous repair by regulating extracellular matrix remodelling.

Sci Rep 2021 Feb 1;11(1):3245. Epub 2021 Feb 1.

Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

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http://dx.doi.org/10.1038/s41598-021-82225-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851382PMC
February 2021

Intracellular alpha-fetoprotein interferes with all-trans retinoic acid induced ATG7 expression and autophagy in hepatocellular carcinoma cells.

Sci Rep 2021 Jan 25;11(1):2146. Epub 2021 Jan 25.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China.

Retinoic acid and retinoid acid receptor (RA-RAR) signaling exhibits suppressive functions in the progression of hepatocellular carcinoma (HCC) through multiple mechanisms. However, whether RA-RAR signaling induces autophagy that contributes its anti-tumor activity in HCC remains elusive. In the current study, the effects of RA-RAR pathway on autophagy were investigated in two HCC cell lines: alpha-fetoprotein (AFP) positive PLC/PRF/5 and AFP negative HLE cells. Cell autophagy was analyzed with western blot for detection of LC3 conversion and p62/SQSTM1 degradation while autophagy flux was assayed using the mRFP-GFP-LC3 reporter. Cell apoptosis and viability were analyzed by caspase-3 activity, TdT-mediated dUTP nick end labeling (TUNEL) assay, and Cell Counting Kit (CCK)-8, respectively. Chromatin immunoprecipitation (ChIP) was employed to detect the binding of RAR onto the promoter of autophagy-relevant 7 (ATG7), and co-immunoprecipitation (CoIP) was used to analyze the interaction of AFP and RAR. The results showed that ATRA dosage and time-dependently induced high levels of cell autophagy in both the PLC/PRF/5 and HLE cells, which was accompanied with up-regulation of ATG7. ChIP assay showed that RAR was able to bind to its responsive elements on ATG7 promoter. Impairment of ATG7 induction or blockade of autophagy with chloroquine aggravated ATRA induced apoptosis of HCC cells. Furthermore, intracellular AFP was able to complex with RAR in PLC/PRF/5 cells. Knockdown of AFP in PLC/PRF/5 cells augmented the up-regulation of ATG7 by ATRA while overexpression of AFP in HLE cells attenuated ATRA induced ATG7 expression and autophagy. Thus, ATRA induced ATG7 and autophagy participated in its cytotoxicity on HCC cells and AFP interfere with the induction of ATG7 and autophagy through forming complex with RAR.
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http://dx.doi.org/10.1038/s41598-021-81678-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835378PMC
January 2021

Taxonomic and functional adaption of the gastrointestinal microbiome of goats kept at high altitude (4800 m) under intensive or extensive rearing conditions.

FEMS Microbiol Ecol 2021 03;97(3)

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.

The gut microbiota composition is influenced by the diet as well as the environment in both wild and domestic animals. We studied the effects of two feeding systems on the rumen and hindgut microbiome of semi-feral Tibetan goats kept at high altitude (∼4800 m) using 16S rRNA gene and metagenomic sequencing. Intensive drylot feeding resulted in significantly higher zootechnical performance, narrower ruminal acetate: propionate ratios and a drop in the average rumen pH at slaughter to ∼5.04. Hindgut microbial adaption appeared to be more diverse in the drylot group suggesting a higher influx of undegraded complex non-starch polysaccharides from the rumen. Despite their higher fiber levels in the diet, grazing goats exhibited lower counts of Methanobrevibacter and genes associated with the hydrogenotrophic methanogenesis pathway, presumably reflecting the scarce dietary conditions (low energy density) when rearing goats on pasture from extreme alpine environments. These conditions appeared to promote a relevant abundance of bacitracin genes. In parallel, we recognized a significant increase in the abundance of antibiotic resistance genes in the digestive tracts of drylot animals. In summary, this study provides a deeper insight into the metataxonomic and functional adaption of the gastrointestinal microbiome of goats subject to intensive drylot and extensive pasture rearing conditions at high altitude.
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http://dx.doi.org/10.1093/femsec/fiab009DOI Listing
March 2021

MdKNOX19, a class II knotted-like transcription factor of apple, plays roles in ABA signalling/sensitivity by targeting ABI5 during organ development.

Plant Sci 2021 Jan 9;302:110701. Epub 2020 Oct 9.

College of Horticulture, Northwest Agriculture and Forestry University, Yangling, Shaanxi, 712100, China; College of Life Sciences, Northwest Agriculture and Forestry University, Yangling, Shaanxi, 712100, China. Electronic address:

The ABI5 transcription factor, which is a core component of the ABA signaling pathway, affects various plant processes, including seed development and germination and responses to environmental cues. The knotted1-like homeobox (KNOX) transcription factor has crucial functions related to plant development, including the regulation of various hormones. In this study, an ABA-responsive KNOX gene, MdKNOX19, was identified in apple (Malus domestica). The overexpression of MdKNOX19 increased the ABA sensitivity of apple calli, resulting in a dramatic up-regulation in the transcription of the Arabidopsis ABI5-like MdABI5 gene. Additionally, MdKNOX19 overexpression in Micro-Tom adversely affected fruit size and seed yield as well as enhanced ABA sensitivity and up-regulated SlABI5 transcription during seed germination and early seedling development. An examination of MdKNOX19-overexpressing Arabidopsis plants also revealed severe defects in seed development and up-regulated expression of ABA-responsive genes. Furthermore, we further confirmed that MdKNOX19 binds directly to the MdABI5 promoter to activate expression. Our findings suggest MdKNOX19 is a positive regulator of ABI5 expression, and the conserved module MdKNOX19-MdABI5-ABA may contribute to organ development.
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http://dx.doi.org/10.1016/j.plantsci.2020.110701DOI Listing
January 2021

Accuracy of intraocular lens calculation formulas in cataract patients with steep corneal curvature.

PLoS One 2020 20;15(11):e0241630. Epub 2020 Nov 20.

Department of Ophthalmology, He Eye Specialists Hospitals, Shenyang, China.

Objective: To compare the accuracy of five kinds of intraocular lens calculation formulas (SRK/T, Haigis, Hoffer Q, Holladay and Barrett Universal Ⅱ) in cataract patients with steep curvature cornea ≥ 46.0 diopters.

Methods: This is a retrospective study of cataract phacoemulsification combined with intraocular lens implantation in patients with steep curvature cornea (corneal curvature ≥ 46D). The refractive prediction errors of IOL power calculation formulas (SRK/T, Haigis, Holladay, Hoffer Q, and Barrett Universal II) using User Group for Laser Interference Biometry (ULIB) constants were evaluated and compared. Objective refraction results were assessed at one month postoperatively. According to axial length (AL), all patients were divided into three groups: short AL group (<22mm), normal AL group (>22 to ≤24.5mm) and long AL group (>24.5mm). Calculate the refractive error and absolute refractive error (AE) between the actual postoperative refractive power and the predicted postoperative refractive power. The covariance analysis was used for the comparison of five formulas in each group. The correlation between the absolute refractive error and AL from every formula were analyzed by Pearson correlation test, respectively.

Result: Total 112 eyes of 83 cataract patients with steep curvature cornea were collected. The anterior chamber depth (ACD) was a covariate in the short AL group in the covariance analysis of absolute refractive error (P<0.001). The SRK/T and Holladay formula had the lowest mean absolute error (MAE) (0.47D), there were statistically significant differences in MAE between the five formulas for short AL group (P = 0.024). The anterior chamber depth had no significant correlation in the five calculation formulas in the normal AL group and long AL group (P = 0.521, P = 0.609 respectively). In the normal AL group, there was no significant difference in MAE between the five calculation formulas (P = 0.609). In the long AL group, Barrett Universal II formula had the lowest MAE (0.35), and there were statistically significant differences in MAE between the five formulas (P = 0.012). Over the entire AL range, the Barrett Universal II formula had the lowest MAE and the highest percentage of eyes within ± 0.50 D, ± 1.00 D, and ± 1.50 D (69.6%, 93.8%, and 98.2% respectively).

Conclusion: Compared to SRK/T, Haigis, Hoffer Q, and Holladay, Barrett Universal Ⅱ formula is more accurate in predicting the IOL power in the cataract patients with steep curvature cornea ≥ 46.0 diopters.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0241630PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678954PMC
January 2021

Genomic identification and expression analysis of nuclear pore proteins in Malus domestica.

Sci Rep 2020 10 15;10(1):17426. Epub 2020 Oct 15.

College of Horticulture, Northwest A&F University, Yangling, China.

The nuclear pore complex (NPC), comprised of individual nucleoporin (Nup) proteins, controls nucleo-cytoplasmic transport of RNA and protein, and is important for regulating plant growth and development. However, there are no reports on this complex in fruit tree species. In this study, we identified 38 apple Nups and named them based on the known Arabidopsis thaliana homologs. We also completed bioinformatics analyses of the intron and exon structural data for apple Nups. The proteins encoded by the apple Nups lacked a universally conserved domain. Moreover, a phylogenetic analysis separated the apple and A. thaliana Nups into three groups. The phylogenetic tree indicated that MdNup54 and MdNup62 are most closely related to genes in other Rosaceae species. To characterize the 38 candidate Malus domestica Nups, we measured their stage-specific expression levels. Our tests revealed these proteins were differentially expressed among diverse tissues. We analyzed the expression levels of seven apple Nups in response to an indole-3-acetic acid (IAA) treatment. The phytohormone treatment significantly inhibited apple flowering. A qRT-PCR analysis proved that an IAA treatment significantly inhibited the expression of these seven genes. A preliminary study regarding two members of the Nup62 subcomplex, MdNup54 and MdNup62, confirmed these two proteins can interact with each other. A yeast two-hybrid assay verified that MdNup54 can interact with MdKNAT4 and MdKNAT6. On the basis of the study results, we identified apple NPC and predicted its structure and function. The data generated in this investigation provide important reference material for follow-up research.
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http://dx.doi.org/10.1038/s41598-020-74171-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7566457PMC
October 2020

Machine learning prediction of the adverse outcome for nontraumatic subarachnoid hemorrhage patients.

Ann Clin Transl Neurol 2020 11 29;7(11):2178-2185. Epub 2020 Sep 29.

Department of Biostatistics & Data Science, School of Public Health, The University of Texas Health Science Center at Houston (UTHealth), Houston, Texas, USA.

Objective: Subarachnoid hemorrhage (SAH) is often devastating with increased early mortality, particularly in those with presumed delayed cerebral ischemia (DCI). The ability to accurately predict survival for SAH patients during the hospital course would provide valuable information for healthcare providers, patients, and families. This study aims to utilize electronic health record (EHR) data and machine learning approaches to predict the adverse outcome for nontraumatic SAH adult patients.

Methods: The cohort included nontraumatic SAH patients treated with vasopressors for presumed DCI from a large EHR database, the Cerner Health Facts EMR database (2000-2014). The outcome of interest was the adverse outcome, defined as death in hospital or discharged to hospice. Machine learning-based models were developed and primarily assessed by area under the receiver operating characteristic curve (AUC).

Results: A total of 2467 nontraumatic SAH patients (64% female; median age [interquartile range]: 56 [47-66]) who were treated with vasopressors for presumed DCI were included in the study. 934 (38%) patients died or were discharged to hospice. The model achieved an AUC of 0.88 (95% CI, 0.84-0.92) with only the initial 24 h EHR data, and 0.94 (95% CI, 0.92-0.96) after the next 24 h.

Interpretation: EHR data and machine learning models can accurately predict the risk of the adverse outcome for critically ill nontraumatic SAH patients. It is possible to use EHR data and machine learning techniques to help with clinical decision-making.
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http://dx.doi.org/10.1002/acn3.51208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664270PMC
November 2020

Highly efficient generation of sheep with a defined FecB mutation via adenine base editing.

Genet Sel Evol 2020 Jul 1;52(1):35. Epub 2020 Jul 1.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.

Base editing has the potential to improve important economic traits in agriculture and can precisely convert single nucleotides in DNA or RNA sequences into minimal double-strand DNA breaks (DSB). Adenine base editors (ABE) have recently emerged as a base editing tool for the conversion of targeted A:T to G:C, but have not yet been used in sheep. ABEmax is one of the latest versions of ABE, which consists of a catalytically-impaired nuclease and a laboratory-evolved DNA-adenosine deaminase. The Booroola fecundity (FecB) mutation (g.A746G, p.Q249R) in the bone morphogenetic protein receptor 1B (BMPR1B) gene influences fecundity in many sheep breeds. In this study, by using ABEmax we successfully obtained lambs with defined point mutations that result in an amino acid substitution (p.Gln249Arg). The efficiency of the defined point mutations was 75% in newborn lambs, since six lambs were heterozygous at the FecB mutation site (g.A746G, p.Q249R), and two lambs were wild-type. We did not detect off-target mutations in the eight edited lambs. Here, we report the validation of the first gene-edited sheep generated by ABE and highlight its potential to improve economically important traits in livestock.
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http://dx.doi.org/10.1186/s12711-020-00554-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7328262PMC
July 2020

Subjective Quality of Vision in Evaporative Dry Eye Patients After Intense Pulsed Light.

Photobiomodul Photomed Laser Surg 2020 Jul 30;38(7):444-451. Epub 2020 Apr 30.

Department of Ophthalmology, He Eye Hospital, Shenyang, China.

Intense pulsed light (IPL) improves visual and clinical symptoms of dry eye disease and helps improve meibomian gland (MG) function thereby positively contributing to the tear lipid layer, a major component for a normal tear film physiology. The objective of this study was to assess the effectiveness of IPL in moderate to severe evaporative dry eye (DE) owing to meibomian gland dysfunction (MGD) on the subjective quality of vision (QOV). This is a prospective, open-label study; patients with moderate to severe evaporative DE owing to MGD were consecutively enrolled and underwent two IPL treatment sessions at 3-week intervals. Clinical assessments included: Ocular Surface Disease Index (OSDI) and QOV questionnaires, noninvasive tear breakup time (NITBUT), interferometric fringe pattern as determined by tear film lipid layer (TFLL) quality, lid margin abnormalities, corneal fluorescein staining (CFS), conjunctival hyperemia (CH) redness score, MG (evaluated with a slit-lamp microscope), best-corrected visual acuity, endothelial cell count, and intraocular pressure were conducted at pretreatment, days 21 and 42 after IPL treatment. A total of 128 eyes of 64 patients (39 women, 25 men; mean age 36.09 ± 11.13 years) with moderate to severe DE owing to MGD consecutively received IPL treatment at days 1 and 21. OSDI ( < 0.05), QOV ( < 0.05), NITBUT ( < 0.05), TFLL score ( < 0.05), and MG quality and expressibility ( < 0.05) improved significantly at day 42, whereas CFS and CH showed minor not significant improvements ( > 0.05) at day 42 of assessment. IPL treatment reduced the severity of DE symptoms and improved the overall tear film stability in patients with moderate to severe evaporative DE owing to MGD. In addition, visual complaints experienced by DE patients secondary to MGD significantly decreased.
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http://dx.doi.org/10.1089/photob.2019.4788DOI Listing
July 2020

Epigenomic Regulatory Mechanism in Vegetative Phase Transition of .

J Agric Food Chem 2020 Apr 15;68(17):4812-4829. Epub 2020 Apr 15.

Key Laboratory of Agricultural Internet of Things, Ministry of Agriculture Rural Affairs, 712100 Yangling, Shaanxi, People's Republic of China.

In woody plants, phase transitions substantially affect growth and development. Although there has been considerable interest in the regulatory mechanisms underlying phase changes, the associated epigenetic modifications remain relatively uncharacterized. We examined the DNA methylation changes and the transcriptional responses in adult and juvenile leaves. The DNA methylations were 66.61% and 68.3% in the CG context, 49.12% and 52.44% in the CHG context, and 7.02% and 8.22% in the CHH context for the adult and juvenile leaves, respectively. The number of differentially methylated regions in all contexts distributed in the genic regions varied. Additionally, inhibited DNA methylation in adult leaves activated the transcription of indole-3-acetic acid related genes in the signaling, response, and transport pathways. Moreover, the opposite methylation and expression patterns were observed for the and family genes between the adult and juvenile leaves. Both gene families contribute to the vegetative phase transition. Furthermore, the hyper-/hypomethylation of the gene body or promoter of transcription factor genes may lead to up-/downregulated gene expression. The methylation levels of the (22), (21), (8), (2), (6), (2), (7), (7), and (4) genes were higher in the adult leaves than in the juvenile leaves, whereas the opposite pattern was observed for the (2), (11), and (3) genes. An analysis of the correlation between methylation and transcription indicated the methylation of the gene body in all contexts and the methylation of the promoter in the CG and CHG contexts are negatively correlated with gene expression. However, the methylation of the promoter in the CHH context is positively correlated with gene expression. These findings reflect the diversity in the epigenetic regulation of gene expression and may be useful for elucidating the epigenetic regulatory mechanism underlying the vegetative phase transition.
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http://dx.doi.org/10.1021/acs.jafc.0c00478DOI Listing
April 2020

Expression and clinical significance of MAPK and EGFR in triple-negative breast cancer.

Oncol Lett 2020 Mar 9;19(3):1842-1848. Epub 2020 Jan 9.

Department of Breast Surgery, The 3rd Affiliated Teaching Hospital of Xinjiang Medical University (Affiliated Cancer Hospital), Urumqi, Xinjiang 830011, P.R. China.

To investigate the expression and clinical significance of mitogen-activated protein kinase (MAPK) and epidermal growth factor receptor (EGFR) in triple-negative breast cancer (TNBC), a total of 300 TNBC and 120 paired paracancerous tissues were examined. Immunohistochemistry was conducted to determine the expression levels of MAPK and EGFR, and the correlation between MAPK and EGFR expression was evaluated using Cramer's V test. The association between MAPK and EGFR expression, and various clinicopathological variables (such as lymph node metastasis, clinical stage, recurrence and metastasis) was also evaluated, using the χ test. MAPK and EGFR expression levels in TNBC tissues were significantly higher than in the paired paracancerous tissues. Moreover, MAPK expression was associated with that of EGFR in TNBC tissues. The positive expression rates of MAPK and EGFR in patients with lymph node metastasis, advanced clinical stage, tumor recurrence and metastasis were higher than those without. Patients with positive expression of MAPK and EGFR in TNBC tissues had poorer prognoses and lower overall survival times than those without expression. In summary, the expression of MAPK and EGFR is closely associated with tumor invasion and the metastasis of TNBC, and may therefore be used as an indicator of poor prognosis in patients with TNBC.
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http://dx.doi.org/10.3892/ol.2020.11274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038935PMC
March 2020

Genome-Wide Identification of the Gene Family and Characterization of Its Transcriptional Regulation in .

Front Plant Sci 2020 21;11:128. Epub 2020 Feb 21.

College of Horticulture, Northwest Agriculture and Forestry University, Yangling, China.

Knotted1-like Homeobox (KNOX) proteins play important roles in regulating plant growth, development, and other biological processes. However, little information is available on the gene family in apple ( Borkh.). In this study, 22 genes were identified in the apple genome. The gene structure, protein characteristics, and promoter region were characterized. The family members were divided into three classes based on their phylogenetic relationships. Quantitative real-time PCR analysis revealed that the majority of genes exhibited strongly preferential expression in buds and were significantly up-regulated during the flower induction period. The transcript levels of genes were responsive to treatments with flowering- and stress-related hormones. The putative upstream regulation factor could directly bind to the promoter of and , and inhibit their transcriptional activities, which were confirmed by yeast one-hybrid and dual-luciferase assays. The results provide an important foundation for future analysis of the regulation and functions of the gene family.
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http://dx.doi.org/10.3389/fpls.2020.00128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7047289PMC
February 2020

Exploring the effect of hypertension on retinal microvasculature using deep learning on East Asian population.

PLoS One 2020 5;15(3):e0230111. Epub 2020 Mar 5.

The Second Clinical College, Dalian Medical University, Dalian, Liaoning, China.

Hypertension is the leading risk factor of cardiovascular disease and has profound effects on both the structure and function of the microvasculature. Abnormalities of the retinal vasculature may reflect the degree of microvascular damage due to hypertension, and these changes can be detected with fundus photographs. This study aimed to use deep learning technique that can detect subclinical features appearing below the threshold of a human observer to explore the effect of hypertension on morphological features of retinal microvasculature. We collected 2012 retinal photographs which included 1007 from patients with a diagnosis of hypertension and 1005 from normotensive control. By method of vessel segmentation, we removed interference information other than retinal vasculature and contained only morphological information about blood vessels. Using these segmented images, we trained a small convolutional neural networks (CNN) classification model and used a deep learning technique called Gradient-weighted Class Activation Mapping (Grad-CAM) to generate heat maps for the class "hypertension". Our model achieved an accuracy of 60.94%, a specificity of 51.54%, a precision of 59.27%, and a recall of 70.48%. The AUC was 0.6506. In the heat maps for the class "hypertension", red patchy areas were mainly distributed on or around arterial/venous bifurcations. This indicated that the model has identified these regions as being the most important for predicting hypertension. Our study suggested that the effect of hypertension on retinal microvascular morphology mainly occurred at branching of vessels. The change of the branching pattern of retinal vessels was probably the most significant in response to elevated blood pressure.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230111PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058325PMC
June 2020

Bionic design and verification of 3D printed PEEK costal cartilage prosthesis.

J Mech Behav Biomed Mater 2020 03 20;103:103561. Epub 2019 Dec 20.

State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, 710054, Xi'an, ShaanXi, China; School of Mechanical Engineering, Xi'an Jiaotong University, 710054, Xi'an, ShaanXi, China. Electronic address:

Chest wall reconstructions are mainly needed after surgical treatment of tumors or trauma. The costal cartilage is part of the chest wall, connecting sternum and ribs. The currently existing rib prostheses made of Titanium or PEEK were found lacking the costal cartilage portion, causing unsatisfactory functional rehabilitation of breath. This study proposed a newly bionic methodology for designing a costal cartilage prosthesis using a wavy elastic structure. By changing the design parameters, the mechanical properties can be accurately adjusted. Finite element analysis was carried out for the optimization of the prostheses. Then the prostheses were fabricated by fused deposition modelling manufacturing technology, using PEEK. Mechanical tests were carried out to determine the elastic modulus of the prostheses. The equivalent modulus ranged 0.5-17.3 MPa, and the tensile strength ranged 0.7-8.3 MPa. The results indicated that the mechanical behavior of the designed prostheses were close to those of the natural costal cartilage and that the wavy elastic structure was a reasonable choice for designing a costal cartilage prosthesis. Therefore, the designed PEEK costal cartilage prostheses have the potential as replacement of the natural costal cartilage with better breathing function for the patient undergoing chest wall reconstruction.
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http://dx.doi.org/10.1016/j.jmbbm.2019.103561DOI Listing
March 2020

Diversity and Composition of Rumen Bacteria, Fungi, and Protozoa in Goats and Sheep Living in the Same High-Altitude Pasture.

Animals (Basel) 2020 Jan 22;10(2). Epub 2020 Jan 22.

Institute of Animal Sciences, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850009, China.

Environmental adaptation of ruminants was highly related to microbiota in the rumen. To investigate the diversity and composition of bacteria, fungi, and protozoa in the rumen of high-altitude animals, amplicon gene sequencing was performed using rumen fluid samples derived from both Tibetan goats and sheep at the same pasture in a highland (altitude > 4800 m). Between these two species, the ruminal bacteria and fungi were significantly different at multiple taxonomic levels. The alpha diversity of bacteria was significantly high in goats ( < 0.05). One hundred and sixty-four and 29 Operational Taxonomy Units (OTUs) with significant differences were detected in bacteria and fungi, respectively. The abundance of bacteria, fungi, and protozoa in the rumen was characterized at multiple taxonomic levels, and we determined that Firmicutes, Bacteroidetes, Neocallimastigomycota, and Ciliophora were the most abundant bacteria, fungi, and protozoa. The family Neocallimastigaceae and the genus Metadinium had cellulose degradation capacity in the rumen with high abundance, thereby, suggesting that fungi and protozoa played an essential role in rumen fermentation. In addition, by comparing microbiota in the rumen of goats and sheep it was found, that the fiber-degrading fungi genus (Cyllamyces) was increased in the rumen of sheep ( < 0.05) whereas VFA-producing bacteria (Saccharofermentans and Lachnospiraceae_XPB1014) were increased in the rumen of goats ( < 0.05). Interestingly, in the rumen, no differences in protozoa were observed between goats and sheep ( > 0.05). Furthermore, when compared to sheep, level of acetic acid, propionic acid, and total volatile fatty acid (TVFA) were significantly increased in the rumen of goats ( < 0.05). Taken together, these results suggested microbiota in the rumen drive goats to better adapt to high-altitude grazing conditions.
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http://dx.doi.org/10.3390/ani10020186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070549PMC
January 2020

Dynamics and stabilization of the rumen microbiome in yearling Tibetan sheep.

Sci Rep 2019 12 23;9(1):19620. Epub 2019 Dec 23.

Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, 810016, China.

The productivity of ruminants depends largely on rumen microbiota. However, there are few studies on the age-related succession of rumen microbial communities in grazing lambs. Here, we conducted 16 s rRNA gene sequencing for bacterial identification on rumen fluid samples from 27 Tibetan lambs at nine developmental stages (days (D) 0, 2, 7, 14, 28, 42, 56, 70, and 360, n = 3). We observed that Bacteroidetes and Proteobacteria populations were significantly changed during the growing lambs' first year of life. Bacteroidetes abundance increased from 18.9% on D0 to 53.9% on D360. On the other hand, Proteobacteria abundance decreased significantly from 40.8% on D0 to 5.9% on D360. Prevotella_1 established an absolute advantage in the rumen after 7 days of age. The co-occurrence network showed that the different microbial of the rumen presented a complex synergistic and cumbersome relationship. A phylogenetic tree was constructed, indicating that during the colonization process, may occur a phenomenon in which bacteria with close kinship are preferentially colonized. Overall, this study provides new insights into the colonization of bacterial communities in lambs that will benefit the development of management strategies to promote colonization of target communities to improve functional development.
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http://dx.doi.org/10.1038/s41598-019-56206-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927978PMC
December 2019

A bifurcated continuous field-flow fractionation (BCFFF) chip for high-yield and high-throughput nucleic acid extraction and purification.

Lab Chip 2019 11 17;19(22):3853-3861. Epub 2019 Oct 17.

Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA. and Center for Microfluidics and Medical Diagnostics, University of Notre Dame, Notre Dame, IN 46556, USA and Harper Cancer Research Institute, University of Notre Dame, Notre Dame, IN 46556, USA and Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.

We report a bifurcated continuous field-flow fractionation (BCFFF) chip for high-yield and high-throughput (20 min) extraction of nucleic acids from physiological samples. The design uses a membrane ionic transistor to sustain low-ionic strength in a localized region at a junction, such that the resulting high field can selectively isolate high-charge density nucleic acids from the main flow channel and insert them into a standardized buffer in a side channel that bifurcates from the junction. The high local electric field and the bifurcated field-flow design facilitate concentration reduction of both divalent cation (Ca) and molecular PCR inhibitors by more than two orders of magnitude, even with high-throughput continuous loading. The unique design with a large (>20 mM mm) on-chip ionic-strength gradient allows miniaturization into a high-throughput field-flow fractionation chip that can be integrated with upstream lysing and downstream PCR/sensor modules for various nucleic acid detection/quantification applications. A concentration-independent 85% yield for extraction and an overall post-PCR yield exceeding 60% are demonstrated for a 111 bp dsDNA in 10 μL of human plasma, compared to no amplification with the raw sample. A net yield four times larger than a commercial extraction kit is demonstrated for miR-39 in human plasma.
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http://dx.doi.org/10.1039/c9lc00818gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982425PMC
November 2019

Extracellular vesicle microRNA quantification from plasma using an integrated microfluidic device.

Commun Biol 2019 20;2:189. Epub 2019 May 20.

1Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556 USA.

Extracellular vesicles (EV) containing microRNAs (miRNAs) have tremendous potential as biomarkers for the early detection of disease. Here, we present a simple and rapid PCR-free integrated microfluidics platform capable of absolute quantification (<10% uncertainty) of both free-floating miRNAs and EV-miRNAs in plasma with 1 pM detection sensitivity. The assay time is only 30 minutes as opposed to 13 h and requires only ~20 μL of sample as oppose to 1 mL for conventional RT-qPCR techniques. The platform integrates a surface acoustic wave (SAW) EV lysing microfluidic chip with a concentration and sensing microfluidic chip incorporating an electrokinetic membrane sensor that is based on non-equilibrium ionic currents. Unlike conventional RT-qPCR methods, this technology does not require EV extraction, RNA purification, reverse transcription, or amplification. This platform can be easily extended for other RNA and DNA targets of interest, thus providing a viable screening tool for early disease diagnosis, prognosis, and monitoring of therapeutic response.
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http://dx.doi.org/10.1038/s42003-019-0435-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527557PMC
April 2020

Comparative RNA-Sequencing and DNA Methylation Analyses of Apple (Malus domestica Borkh.) Buds with Diverse Flowering Capabilities Reveal Novel Insights into the Regulatory Mechanisms of Flower Bud Formation.

Plant Cell Physiol 2019 Aug;60(8):1702-1721

Ministry of Agriculture Rural Affairs, Key Laboratory of Agricultural Internet of Things, Yangling, Shaanxi, P. R. China.

In plants, DNA methylation (i.e. chromatin modification) is important for various biological processes, including growth, development and flowering. Because 'Fuji' apple trees are alternate bearing and have a long ripening period and poor-quality flower buds, we used bud types with diverse flowering capabilities to investigate the epigenetic regulatory mechanisms influencing flower bud formation. We examined the DNA methylation changes and the transcriptional responses in the selected apple bud types. We observed that in the apple genome, approximately 79.5%, 67.4% and 23.7% of the CG, CHG and CHH sequences are methylated, respectively. For each sequence context, differentially methylated regions exhibited distinct methylation patterns among the analyzed apple bud types. Global methylation and transcriptional analyses revealed that nonexpressed genes or genes expressed at low levels were highly methylated in the gene-body regions, suggesting that gene-body methylation is negatively correlated with gene expression. Moreover, genes with methylated promoters were more highly expressed than genes with unmethylated promoters, implying promoter methylation and gene expression are positively correlated. Additionally, flowering-related genes (e.g. SOC1, AP1 and SPLs) and some transcription factor genes (e.g. GATA, bHLH, bZIP and WOX) were highly expressed in spur buds (highest flowering rate), but were associated with low methylation levels in the gene-body regions. Our findings indicate a potential correlation between DNA methylation and gene expression in apple buds with diverse flowering capabilities, suggesting an epigenetic regulatory mechanism influences apple flower bud formation.
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http://dx.doi.org/10.1093/pcp/pcz080DOI Listing
August 2019

Peripheral vessel and air bronchograms for detecting the pathologic patterns of subsolid nodules.

Clin Imaging 2019 Jul - Aug;56:63-68. Epub 2019 Mar 22.

Department of Pathology, the First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei, China.

Purpose: To assess the relationships of subsolid nodules (SSNs) with peripheral vessels and aerated bronchi using computed tomography (CT), and to correlate the imaging features with the benign/malignant pathological diagnoses.

Methods: This study retrospectively analyzed data from 83 patients with a solitary SSN (January 2008 to December 2016). SSNs were imaged (LightSpeed 64-slice spiral CT, General Electric, USA), their mean diameter determined, and the relationship with peripheral vessels (types I-IV) and aerated bronchi (types I-V) were classified. Pathologic diagnoses were obtained from the surgical specimens.

Results: SSNs were diagnosed as benign (n = 29), pre-invasive (n = 9), micro-invasive adenocarcinoma (n = 7) and invasive adenocarcinoma (n = 38). SSN size, peripheral vessel class and aerated bronchus class differed between pathologic types (P < 0.05). For benign SSNs, peripheral vessel type II (58.6%) was most common, followed by III (20.7%) and IV (6.9%). Aerated bronchus type V (65.5%) was most frequent, followed by IV (27.6%); type I aerated bronchus was not observed. No cases of micro-invasive or invasive adenocarcinoma were peripheral vessel type I or aerated bronchus type V. For invasive adenocarcinoma, 92.1% were peripheral vessel types III + IV while 71.8% were aerated bronchus types I + II.

Conclusions: SSN pathologic types differ with regard to peripheral vessel and aerated bronchus types. Type I peripheral vessel and type V aerated bronchus (both least involved) suggest a benign lesion, whereas type III/IV peripheral vessel and type I/II aerated bronchus (both most involved) suggest malignancy.
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http://dx.doi.org/10.1016/j.clinimag.2019.03.010DOI Listing
December 2019

Facile and Large-Area Preparation of Polypyrrole Film for Low-Haze Transparent Supercapacitors.

ACS Appl Mater Interfaces 2018 Dec 19;10(48):41299-41311. Epub 2018 Nov 19.

The transparent flexible supercapacitor is considered to be the key energy-storage component for the development of wearable and fully transparent electronic devices. However, the current transparent supercapacitor faces the low-haze challenge, which is essential for the high-definition visualization in transparent electronics. Herein, we developed a facile interfacial polymerization approach for the large-area preparation of flexible polypyrrole/polyethylene terephthalate (PPy/PET) transparent conductive films in a cost-effective way. The PPy/PET film exhibits a highly uniform morphology and a low haze level of 1.40% (corresponding to high definition) as well as negligible resistance changing under an ultrasmall bending radius. The sandwich-structured, large-area, transparent supercapacitor assembled based on the PPy/PET films also keeps a similar low haze level. A facile N, N-dimethylformamide etchant-written strategy on the PPy/PET film is developed to fabricate the patterned micro-supercapacitors (MSCs) in series in scalable area, which show a low haze level of 1.66% and a high transparency of 70.2%. Significantly, the low-haze MSC possesses high energy-storage capacity and presents almost no capacitance loss at an extreme bending state. This work demonstrates a facile preparation of large-area and low-haze transparent flexible supercapacitors and also enlightens broad interests in their potential integrity toward the fully transparent wearable electronics.
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http://dx.doi.org/10.1021/acsami.8b13503DOI Listing
December 2018

Identification of Nilaparvata lugens and Its Two Sibling Species (N. bakeri and N. muiri) by Direct Multiplex PCR.

J Econ Entomol 2018 12;111(6):2869-2875

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou,, China.

The brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is a destructive rice pest of Asia. Currently, one important monitoring method of BPH is through black light trapping. However, two sibling species of BPH, Nilaparvata bakeri (Muri) and Nilaparvata muiri China, can also be trapped by black light, and these species feed only on gramineous weeds rather than on rice. Therefore, the accurate identification of Nilaparvata species is crucial for N. lugens forecasting and management. The traditional morphological identification method is not feasible for subadults and damaged specimens. Furthermore, this error-prone morphological identification method is time and labor intensive, with the need for expertise and experience. Here, we established a direct multiplex polymerase chain reaction (dmPCR) assay using crude tissue fluid as a template, omitting purified DNA extraction. The crude tissue fluid can be obtained by grinding specimens without any biological reagent but only using distilled water. This dmPCR assay, using three pairs of diagnostic primers, is based on internal transcribed spacers (ITS). Each primer pair amplifies a species-specific fragment of a different size, which were easily and reliably separated in a 2% agarose gel. Furthermore, the dmPCR was verified to be applicable to damaged tissue specimens, such as head, thorax, or abdomen. In conclusion, this dmPCR assay is a novel, time-saving, cost-effective, and easy-to-apply molecular diagnostic method for the identification of the above three sibling species, N. lugens, N. bakeri, and N. muiri.
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http://dx.doi.org/10.1093/jee/toy232DOI Listing
December 2018

Author Correction: Exosomes secreted by human adipose mesenchymal stem cells promote scarless cutaneous repair by regulating extracellular matrix remodelling.

Sci Rep 2018 May 1;8(1):7066. Epub 2018 May 1.

Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
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http://dx.doi.org/10.1038/s41598-018-24991-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931573PMC
May 2018
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