Publications by authors named "Chao Chen"

2,532 Publications

  • Page 1 of 1

[Short term clinical observation of cervical anterior Hybrid surgery].

Zhongguo Gu Shang 2022 Aug;35(8):740-6

Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China.

Objective: To investigate the short-term clinical effect of the cervical anterior Hybrid surgery in the treatment of two-segment and three-segment cervical spondylosis.

Methods: From January 2018 to January 2019, 108 patients who were performed anterior Hybrid surgery with cervical degenerative diseases were collected. The patients were divided into a two-segment group with 52 patients and a three-segment group with 56 patients according to surgical segments. In two-segment group, there were 24 males and 28 females, aged from 35 to 67 years old with an average of(45.94±14.67) years old. In three-segment group, there were 23 males and 33 females, aged from 32 to 65 years old with an average of (47.54±15.34) years old. The outcome indicators of the two groups were compared. Clinical indicators:neck disability index(NDI) was used to evaluate daily life ability, Japanese Orthopedic Association(JOA) score was used to evaluate neurological function improvement, visual analogue scale(VAS) was used to evaluate pain intensity, and general clinical results were graded according to Odom's score. Cervical range of motion (ROM), fusion and complications were measured by X-ray, CT and MRI.

Results: All operations were successfully completed and all patients were followed up for more than 12 months. The operation time of two-segment group and three-segment group were 95 to 180 min with an average of(152.30±44.74) min and 110 to 210 min with an average of (165.18±45.86) mins, the blood loss were 20 to 100 ml with an average of (32.88±8.75) ml and 20 to 150 ml with an average of(34.64±10.63) ml respectively which has no statistical differences between the two groups (>0.05). Compared with those before surgery, NDI, JOA, VAS and Odom's scores between two groups were significantly improved at 12 months after operation(<0.05). However, there was no significant difference in the NDI, JOA and Odom's scores between two groups (>0.05), and VAS in three-segment group was higher than that in two-segment group. There was no significant difference in C-C cervical mobility between two groups. Surgical incisions healed smoothly in all patients without complication such as spinal cord injury and cerebrospinal fluid leakage. The bone fusion of the two groups were 43 cases (82.69%) and 45 cases(80.35%) respectively. In two-segment group, there were 2 cases of adjacent segmental hyperosteogeny, and there were 3 cases of adjacent segmental hyperosteogeny and 1 case of adjacent posterior longitudinal ligament ossification in the three-segment group. In addition, in three-segment group, there was 1 case of looseness of implants with no obvious clinical symptoms.

Conclusion: The anterior Hybrid surgery in treating multi-level cervical spondylosis could not only improve clinical symptoms of patients but also preserve mobility. Meanwhile, the efficacy and safety of Hybrid surgery in different multi-level cervical disc diseases are confirmed, proving its value in clinical practice.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2022.08.008DOI Listing
August 2022

LiI/Cu Mixed Conductive Interface via the Mechanical Rolling Approach for Stable Lithium Anodes in the Carbonate Electrolyte.

ACS Appl Mater Interfaces 2022 Aug 17. Epub 2022 Aug 17.

Guangzhou Key Laboratory for Surface Chemistry of Energy Materials, New Energy Research Institute, School of Environment and Energy, South China University of Technology, Guangzhou 510006, P.R. China.

The nonuniform ion/charge distribution and slow Li-ion diffusion at the Li metal/electrolyte interface lead to uncontrollable dendrites growth and inferior cycling stability. Herein, a simple mechanical rolling method is introduced to construct a mixed conductive protective layer composed of LiI and Cu on the Li metal surface through the replacement reaction between CuI nanoflake arrays and metallic Li. LiI can promote Li transportation across the interface, achieving homogeneous Li flux and suppressing the growth of Li dendrite, while the homogeneously dispersed Cu nanoparticles can offer abundant nucleation sites for Li deposition, resulting in a remarkably homogenized charge distribution. As expected, Li metal with the LiI/Cu protection layer (LiI/[email protected]) exhibits a significantly prolonged lifespan over 350 h with slight polarization at a deposition capacity of 3 mAh cm in the carbonate electrolyte. Besides, when matched with high mass loading LiFePO cathodes (20 mg cm), the LiI/[email protected] anodes exhibit much improved cycle stability and rate performance. Highly scalable preparation processes as well as the impressive electrochemical performances in half cells and full cells indicate the potential application of the LiI/[email protected] anode.
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http://dx.doi.org/10.1021/acsami.2c11632DOI Listing
August 2022

Review of the toxicity and potential molecular mechanisms of parental or successive exposure to environmental pollutants in the model organism Caenorhabditis elegans.

Environ Pollut 2022 Aug 12;311:119927. Epub 2022 Aug 12.

Institute for Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, PR China.

Environmental pollutants such as heavy metals, nano/microparticles, and organic compounds have been detected in a wide range of environmental media, causing long-term exposure in various organisms and even humans through breathing, contacting, ingestion, and other routes. Long-term exposure to environmental pollutants in organisms or humans promotes exposure of offspring to parental and environmental pollutants, and subsequently results in multiple biological defects in the offspring. This review dialectically summarizes and discusses the existing studies using Caenorhabditis elegans (C. elegans) as a model organism to explore the multi/transgenerational toxicity and potential underlying molecular mechanisms induced by environmental pollutants following parental or successive exposure patterns. Parental and successive exposure to environmental pollutants induces various biological defects in C. elegans across multiple generations, including multi/transgenerational developmental toxicity, neurotoxicity, reproductive toxicity, and metabolic disturbances, which may be transmitted to progeny through reactive oxygen species-induced damage, epigenetic mechanisms, insulin/insulin-like growth factor-1 signaling pathway. This review aims to arouse researchers' interest in the multi/transgenerational toxicity of pollutants and hopes to explore the possible long-term effects of environmental pollutants on organisms and even humans, as well as to provide constructive suggestions for the safety and management of emerging alternatives.
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http://dx.doi.org/10.1016/j.envpol.2022.119927DOI Listing
August 2022

CD147 mediates epidermal malignant transformation through the RSK2/AP-1 pathway.

J Exp Clin Cancer Res 2022 Aug 13;41(1):246. Epub 2022 Aug 13.

Department of Dermatology, Xiangya Hospital, Central South University; Hunan Key Laboratory of Skin Cancer and Psoriasis and Hunan Engineering Research Center of Skin Health and Disease; National Clinical Research Center for Geriatric Disorders (Xiangya Hospital), Xiangya Road #87, Changsha, 410008, Hunan, China.

Background: Malignant transformation of the epidermis is an essential process in the pathogenesis of cutaneous squamous-cell carcinoma (cSCC). Although evidence has demonstrated that CD147 plays key roles in various tumors, the role of CD147 in epidermal malignant transformation in vivo remains unclear.

Methods: Epidermal CD147-overexpression or knockout (Epi or Epi) transgenic mouse models were generated for in vivo study. RNA-sequencing and q-PCR were performed to identify the differentially expressed genes. Immunohistochemistry and flow cytometry were performed to investigate the role of CD147 in regulating myeloid-derived suppressor cells (MDSCs). Immunoprecipitation, EMSA and ChIP assays were performed to investigate the mechanism of CD147 in cell transformation.

Results: We found that specific overexpression of CD147 in the epidermis (Epi) induces spontaneous tumor formation; moreover, a set of chemokines and cytokines including CXCL1, which play essential function in MDSC recruitment, were significantly upregulated in Epi transgenic mice. As expected, overexpression of CD147 in the epidermis remarkably facilitated tumorigenesis by increasing the rate of tumor initiation and the number and size of tumors in the DMBA/TPA mouse model. Interestingly, the expression of CXCL1 and the infiltration of MDSCs were dramatically increased in Epi transgenic mice. Our findings also showed that knockdown of CD147 attenuated EGF-induced malignant transformation as well as CXCL1 expression in HaCaT cells. Consistently, CD147 was found overexpressed in cutaneous squamous cell carcinoma (cSCC), and positively related with the expression of CD33, a myeloid-associated marker. We further identified RSK2, a serine/threonine kinase, as an interacting partner of CD147 at the binding site of CD147. The interaction of CD147 and RSK2 activated RSK2, thus enhancing AP-1 transcriptional activation. Furthermore, EMSAs and ChIP assays showed that AP-1 could associate with the CXCL1 promoter. Importantly, RSK2 inhibitor suppressed the tumor growth in DMBA/TPA mouse model by inhibiting the recruitment of MDSCs.

Conclusion: Our findings demonstrate that CD147 exerts a key function in epidermal malignant transformation in vivo by activating keratinocytes and recruiting MDSCs via the RSK2/AP-1 pathway.
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http://dx.doi.org/10.1186/s13046-022-02427-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375950PMC
August 2022

Steady-state visually evoked potential collaborative BCI system deep learning classification algorithm based on multi-person feature fusion transfer learning-based convolutional neural network.

Front Neurosci 2022 26;16:971039. Epub 2022 Jul 26.

Key Laboratory of Complex System Control Theory and Application, Tianjin University of Technology, Tianjin, China.

Objective: The conventional single-person brain-computer interface (BCI) systems have some intrinsic deficiencies such as low signal-to-noise ratio, distinct individual differences, and volatile experimental effect. To solve these problems, a centralized steady-state visually evoked potential collaborative BCI system (SSVEP-cBCI), which characterizes multi-person electroencephalography (EEG) feature fusion was constructed in this paper. Furthermore, three different feature fusion methods compatible with this new system were developed and applied to EEG classification, and a comparative analysis of their classification accuracy was performed with transfer learning-based convolutional neural network (TL-CNN) approach.

Approach: An EEG-based SSVEP-cBCI system was set up to merge different individuals' EEG features stimulated by the instructions for the same task, and three feature fusion methods were adopted, namely parallel connection, serial connection, and multi-person averaging. The fused features were then input into CNN for classification. Additionally, transfer learning (TL) was applied first to a Tsinghua University (THU) benchmark dataset, and then to a collected dataset, so as to meet the CNN training requirement with a much smaller size of collected dataset and increase the classification accuracy. Ten subjects were recruited for data collection, and both datasets were used to gauge the three fusion algorithms' performance.

Main Results: The results predicted by TL-CNN approach in single-person mode and in multi-person mode with the three feature fusion methods were compared. The experimental results show that each multi-person mode is superior to single-person mode. Within the 3 s time window, the classification accuracy of the single-person CNN is only 90.6%, while the same measure of the two-person parallel connection fusion method can reach 96.6%, achieving better classification effect.

Significance: The results show that the three multi-person feature fusion methods and the deep learning classification algorithm based on TL-CNN can effectively improve the SSVEP-cBCI classification performance. The feature fusion method of multi -person parallel feature connection achieves better classification results. Different feature fusion methods can be selected in different application scenarios to further optimize cBCI.
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http://dx.doi.org/10.3389/fnins.2022.971039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360603PMC
July 2022

A translational mouse model for investigation of the mechanism of preterm diffuse white matter injury.

Transl Pediatr 2022 Jul;11(7):1074-1084

Department of Neonatology, Children's Hospital of Fudan University, Shanghai, China.

Background: The increasing incidence of preterm birth has led to a global problem of adverse neurodevelopmental outcomes in preterm neonates as a result of brain injury. There is still a lack of models mimicking diffuse white matter injury (WMI) in preterm neonates that can be applied to transgenic mice.

Methods: The right common carotid artery of the neonatal mouse was ligated on postnatal day 3 (P3) C57BL/6 mice and followed by 80, 90, or 100 min of hypoxia using a mixture of 10%±0.2% oxygen-nitrogen. The most suitable model was chosen by characterizing the effects of this hypoxic-ischemic insult on development of myelin, glial cell conditions, and neurological outcomes by hematoxylin-eosin (HE) staining performed at postnatal day 17 (P17), western blot measuring myelin basic protein (MBP) at postnatal day 10 (P10) and P17, immunofluorescence staining of MBP-neurofilament protein heavy chain (NFH), oligodendrocyte transcription factor-2 (Olig2)-adenomatous polyposis coli clone (CC1), glial fibrillary acidic protein (GFAP) and ionic calcium linker protein (Iba-1) at P17, electron microscopy observing myelin microstructure at postnatal day 52 (P52) and behavioral testing at postnatal day 45-50 (P45-P50).

Results: The 90-min group showed neuroanatomical changes in the ipsilateral side of the brain, the 80-min group showed minor changes, and the 100-min group showed severe injury. Mice in the 90-min group subsequently showed marked activation of astrocytes, augmentation of microglia, a notable decrease in expression of MBP with a normal level of NFH, long-term cognitive dysfunction, and impairment of the myelin ultrastructure in adulthood.

Conclusions: In conclusion, a mouse model of preterm diffuse WMI rather than cystic periventricular leukomalacia was successfully achieved by ligating one of the common carotid arteries on P3 followed by 90 min of hypoxia in a mixture of 10%±0.2% oxygen-nitrogen. The attempt provides an adequate translational animal model for elucidating the underlying mechanism.
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http://dx.doi.org/10.21037/tp-22-58DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360811PMC
July 2022

Improving Network-Based Anomaly Detection in Smart Home Environment.

Sensors (Basel) 2022 Jul 27;22(15). Epub 2022 Jul 27.

Discipline of Information Technology, College of Science & Engineering, James Cook University, Townsville, QLD 4811, Australia.

The Smart Home (SH) has become an appealing target of cyberattacks. Due to the limitation of hardware resources and the various operating systems (OS) of current SH devices, existing security features cannot protect such an environment. Generally, the traffic patterns of an SH IoT device under attack often changes in the Home Area Network (HAN). Therefore, a Network-Based Intrusion Detection System (NIDS) logically becomes the forefront security solution for the SH. In this paper, we propose a novel method to assist classification machine learning algorithms generate an anomaly-based NIDS detection model, hence, detecting the abnormal SH IoT device network behaviour. Three network-based attacks were used to evaluate our NIDS solution in a simulated SH test-bed environment. The detection model generated by traditional and ensemble classification Mechanical Learning (ML) methods shows outstanding overall performance. The accuracy of all detection models is over 98.8%.
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http://dx.doi.org/10.3390/s22155626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370897PMC
July 2022

Nontraumatic convexal subarachnoid hemorrhage: A case report.

World J Clin Cases 2022 Jun;10(18):6205-6210

Department of Neurology, Binzhou Medical University Hospital, Binzhou 256600, Shandong Province, China.

Background: Nontraumatic convexal subarachnoid hemorrhage (cSAH) is a rare type of atypical subarachnoid hemorrhage. It mainly presents as a focal and transient neurological deficit with similar manifestations as transient ischemic attack.

Case Summary: We report a case of a 64-year-old man who visited the hospital with paroxysmal left-sided numbness and weakness is presented in this study. Computed tomography examination indicated a high-density image of the right frontal-parietal sulcus. Digital subtraction angiography showed severe stenosis at the right anterior cerebral artery A2-A3 junction (stenosis rate approximately 70%).

Conclusion: The findings of this case indicate that anterior cerebral artery stenosis may lead to the occurrence of cSAH.
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http://dx.doi.org/10.12998/wjcc.v10.i18.6205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254175PMC
June 2022

Effect of Intranasal Dexmedetomidine or Midazolam for Premedication on the Occurrence of Respiratory Adverse Events in Children Undergoing Tonsillectomy and Adenoidectomy: A Randomized Clinical Trial.

JAMA Netw Open 2022 Aug 1;5(8):e2225473. Epub 2022 Aug 1.

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu, China.

Importance: Perioperative respiratory adverse events (PRAEs) are the most common complication during pediatric anesthesia, and they may be affected by the administration of preoperative sedatives.

Objective: To investigate the effect of intranasal dexmedetomidine or midazolam used for premedication on the occurrence of PRAEs.

Design, Setting, And Participants: This single-center, double-blind, randomized clinical trial was conducted among children aged 0 to 12 years undergoing elective tonsillectomy and adenoidectomy from October 2020 to June 2021 at Children's Hospital of Xuzhou Medical University, Xuzhou, China. Data analysis was performed from June to October 2021.

Interventions: Children were randomly assigned to 3 groups: the midazolam group received intranasal midazolam (0.1 mg/kg), and the dexmedetomidine group received intranasal dexmedetomidine (2.0 μg/kg) for premedication. The normal saline group received intranasal 0.9% saline for control.

Main Outcomes And Measures: The primary outcome was the difference in the incidence of PRAEs among the 3 groups. The secondary outcomes were the frequency of the individual PRAEs, including the incidence of such events during the induction and recovery periods, postoperative emergence delirium, postoperative pain score, sedation success rate, and heart rate values.

Results: A total of 384 children (median [IQR] age, 7 [5-10] years; 227 boys [59.1%]) were enrolled and randomized; 373 data sets were available for intention-to-treat analysis (124 children in the midazolam group, 124 children in the dexmedetomidine group, and 125 children in the normal saline group). After the data were adjusted for age, sex, American Society of Anesthesiologists physical status, body mass index, obstructive sleep apnea, upper respiratory tract infection, and passive smoking, children in the midazolam group were more likely to experience PRAEs than those in the normal saline group (70 of 124 children [56.5%] vs 51 of 125 children [40.8%]; adjusted odds ratio [aOR], 1.99; 95% CI, 1.18-3.35), whereas the dexmedetomidine group had a significantly lower PRAEs incidence than the normal saline group (30 of 124 children [24.2%] vs 51 of 125 children [40.8%]; aOR, 0.45; 95% CI, 0.26-0.78). Compared with the dexmedetomidine group, the midazolam group had a higher risk of PRAEs (aOR, 4.44; 95% CI, 2.54-7.76), but no other serious clinical adverse events were observed.

Conclusions And Relevance: In this randomized clinical trial, intranasal midazolam used for premedication was associated with increased incidence of PRAEs, whereas premedication with intranasal dexmedetomidine was associated with reduced incidence of PRAEs. Where clinically appropriate, anesthesiologists should consider using intranasal dexmedetomidine for sedation in children undergoing tonsillectomy and adenoidectomy.

Trial Registration: Chinese Clinical Trial Register Identifier: ChiCTR2000038359.
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http://dx.doi.org/10.1001/jamanetworkopen.2022.25473DOI Listing
August 2022

Topological Spin Texture of Chiral Edge States in Photonic Two-Dimensional Quantum Walks.

Phys Rev Lett 2022 Jul;129(4):046401

Hefei National Laboratory for Physical Sciences at the Microscale and Department of Modern Physics, University of Science and Technology of China, Shanghai Branch, Shanghai 201315, China.

Topological insulators host topology-linked boundary states, whose spin and charge degrees of freedom could be exploited to design topological devices with enhanced functionality. We experimentally observe that dissipationless chiral edge states in a spin-orbit coupled anomalous Floquet topological phase exhibit topological spin texture on boundaries, realized via a two-dimensional quantum walk. Our experiment shows that, for a walker traveling around a closed loop along the boundary in real space, its spin evolves and winds through a great circle on the Bloch sphere, which implies that edge-spin texture has nontrivial winding. This topological spin winding is protected by a chiral-like symmetry emerging for the low-energy Hamiltonian. Our experiment confirms that two-dimensional anomalous Floquet topological systems exhibit topological spin texture on the boundary, which could inspire novel topology-based spintronic phenomena and devices.
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http://dx.doi.org/10.1103/PhysRevLett.129.046401DOI Listing
July 2022

Fusion of CT images and clinical variables based on deep learning for predicting invasiveness risk of stage I lung adenocarcinoma.

Med Phys 2022 Aug 8. Epub 2022 Aug 8.

Department of Interventional Radiology, Fudan University Shanghai Cancer Center, Xuhui District, Shanghai, China.

Purpose: To develop a novel multimodal data fusion model by incorporating computed tomography (CT) images and clinical variables based on deep learning for predicting the invasiveness risk of stage I lung adenocarcinoma that manifests as ground-glass nodules (GGNs) and compare the diagnostic performance of it with that of radiologists.

Methods: A total of 1946 patients with solitary and histopathologically confirmed GGNs with maximum diameter less than 3 cm were retrospectively enrolled. The training dataset containing 1704 GGNs was augmented by resampling, scaling, random cropping, and so forth, to generate new training data. A multimodal data fusion model based on residual learning architecture and two multilayer perceptron with attention mechanism combining CT images with patient general data and serum tumor markers was built. The distance-based confidence scores (DCS) were calculated and compared among multimodal data models with different combinations. An observer study was conducted and the prediction performance of the fusion algorithms was compared with that of the two radiologists by an independent testing dataset with 242 GGNs.

Results: Among the whole GGNs, 606 GGNs are confirmed as invasive adenocarcinoma (IA) and 1340 are non-IA. The proposed novel multimodal data fusion model combining CT images, patient general data, and serum tumor markers achieved the highest accuracy (88.5%), area under a ROC curve (0.957), F1 (81.5%), F1 (81.9%), and Matthews correlation coefficient (73.2%) for classifying between IA and non-IA GGNs, which was even better than the senior radiologist's performance (accuracy, 86.1%). In addition, the DCSs for multimodal data suggested that CT image had a stronger influence (0.9540) quantitatively than general data (0.6726) or tumor marker (0.6971).

Conclusion: This study demonstrated that the feasibility of integrating different types of data including CT images and clinical variables, and the multimodal data fusion model yielded higher performance for distinguishing IA from non-IA GGNs.
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http://dx.doi.org/10.1002/mp.15903DOI Listing
August 2022

Discovery of 1-(Hetero)aryl-β-carboline Derivatives as IDO1/TDO Dual Inhibitors with Antidepressant Activity.

J Med Chem 2022 Aug 7. Epub 2022 Aug 7.

Key Laboratory of Cardiovascular & Cerebrovascular Medicine, Drug Target and Drug Discovery Center, School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.

Depression is the leading cause of global burden of disease and disability. Abnormalities in the kynurenine pathway of tryptophan degradation have been closely linked to the pathogenesis of depression. An integrative bioinformatics analysis demonstrated that indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan 2,3-dioxygenase (TDO) are potential targets for the development of antidepressants. A series of 1-(hetero)aryl-β-carboline derivatives were designed, synthesized, and evaluated as novel IDO1/TDO dual inhibitors. Among them, compound displayed potent inhibition of both IDO1 (IC = 3.53 μM) and TDO (IC = 1.15 μM) and had an acceptable safety profile and pharmacokinetic properties. Compound also rescued lipopolysaccharide-induced depressive-like behavior in mice. Further studies revealed that likely had unique antidepressant mechanisms involving suppressing microglial activation, lowering IDO1 expression, and reducing proinflammatory cytokine and kynurenine levels in the mouse brain. Overall, this work provides practical guidance for the development of IDO1/TDO dual inhibitors to treat inflammation-induced depression.
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http://dx.doi.org/10.1021/acs.jmedchem.2c00677DOI Listing
August 2022

Relative team-member exchange, affective organizational commitment and innovative behavior: The moderating role of team-member exchange differentiation.

Front Psychol 2022 22;13:948578. Epub 2022 Jul 22.

School of Management, Xi'an Jiaotong University, Xi'an, China.

Based upon social comparison theory, a multilevel moderated-mediation theoretical model was built up to explore the influence mechanism of relative team-member exchange (RTMX) on innovative behavior. We tested the proposed hypotheses using a sample of 260 individual members within 51 teams in a two-wave survey study. Controlling for team-member exchange (TMX), results showed that RTMX was positively related to innovative behavior, and the relationship above was mediated by affective organizational commitment. Moreover, team-level TMX differentiation played a moderating role in the mediated relationship between RTMX and innovative behavior through affective organizational commitment. This study also emphasizes the significance of conceptualizing TMX as concurrently implementing at multiple levels.
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http://dx.doi.org/10.3389/fpsyg.2022.948578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355244PMC
July 2022

Effect of essential oil as an additive on the chemical composition, fermentation quality, and bacterial community of paper mulberry silage.

Front Microbiol 2022 22;13:951958. Epub 2022 Jul 22.

College of Animal Science, Guizhou University, Guiyang, China.

Paper mulberry ( L., PM) is being used as a new type of animal protein feed to address the feed crisis. To investigate the effect of additives on the chemical composition, fermentation quality, and bacterial community of PM silage (at room temperature, 25°), paper mulberry was fermented with formic acid (FA), essential oil (AVEO) and lactic acid bacteria (LAB) inoculant treatments. The results showed that fresh PM had a low water-soluble carbohydrate (WSC) content and large amounts of unclassified bacteria. Compared with the CK and LAB treatments, the FA and AVEO treatments significantly ( < 0.05) decreased the pH and increased the lactic acid content of PM silage after 60 days of ensiling. In the AVEO-treated silages the abundance of in the early stage of ensiling increased by 14.09%, the abundances of and in the late stage of ensiling increased by 58.34 and 91.12%, respectively, and the abundance of decreased by 94.71%, resulting in improved PM silage quality. These results confirmed that AVEO could potentially be developed as a new additive for improving the fermentation quality of silage.
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http://dx.doi.org/10.3389/fmicb.2022.951958DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355139PMC
July 2022

Caveolin-1 identified as a key mediator of acute lung injury using bioinformatics and functional research.

Cell Death Dis 2022 Aug 6;13(8):686. Epub 2022 Aug 6.

Department of Physiology, Hunan Normal University School of Medicine, Changsha, 410013, China.

Acute lung injury (ALI) is a potentially life-threatening, devastating disease with an extremely high rate of mortality. The underlying mechanism of ALI is currently unclear. In this study, we aimed to confirm the hub genes associated with ALI and explore their functions and molecular mechanisms using bioinformatics methods. Five microarray datasets available in GEO were used to perform Robust Rank Aggregation (RRA) to identify differentially expressed genes (DEGs) and the key genes were identified via the protein-protein interaction (PPI) network. Lipopolysaccharide intraperitoneal injection was administered to establish an ALI model. Overall, 40 robust DEGs, which are mainly involved in the inflammatory response, protein catabolic process, and NF-κB signaling pathway were identified. Among these DEGs, we identified two genes associated with ALI, of which the CAV-1/NF-κB axis was significantly upregulated in ALI, and was identified as one of the most effective targets for ALI prevention. Subsequently, the expression of CAV-1 was knocked down using AAV-shCAV-1 or CAV-1-siRNA to study its effect on the pathogenesis of ALI in vivo and in vitro. The results of this study indicated that CAV-1/NF-κB axis levels were elevated in vivo and in vitro, accompanied by an increase in lung inflammation and autophagy. The knockdown of CAV-1 may improve ALI. Mechanistically, inflammation was reduced mainly by decreasing the expression levels of CD3 and F4/80, and activating autophagy by inhibiting AKT/mTOR and promoting the AMPK signaling pathway. Taken together, this study provides crucial evidence that CAV-1 knockdown inhibits the occurrence of ALI, suggesting that the CAV-1/NF-κB axis may be a promising therapeutic target for ALI treatment.
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http://dx.doi.org/10.1038/s41419-022-05134-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357074PMC
August 2022

The variation of antenatal corticosteroids administration for the singleton preterm birth in China, 2017 to 2018.

BMC Pediatr 2022 08 3;22(1):469. Epub 2022 Aug 3.

Department of Neonatology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, #2699, Gaoke western Road, Pudong District, Shanghai, 201204, China.

Background: The administration of antenatal corticosteroids (ACS) to women who are at risk of preterm birth has been proven to reduce not only the mortality, but also the major morbidities of the preterm infants. The rate of ACS and the risk factors associated with ACS use in Chinese population is unclear. This study aimed to investigate the rate of ACS use and the associated perinatal factors in the tertiary maternal centers of China.

Methods: Data for this retrospective observational study came from a clinical database of preterm infants established by REIN-EPIQ trial. All infants born at < 34 weeks of gestation and admitted to 18 tertiary maternal centers in China from 2017 to 2018 were enrolled. Any dose of dexamethasone was given prior to preterm delivery was recorded and the associated perinatal factors were analyzed.

Results: The rate of ACS exposure in this population was 71.2% (range 20.2 - 92%) and the ACS use in these 18 maternal centers varied from 20.2 to 92.0% in this period. ACS exposure was higher among women with preeclampsia, caesarean section delivery, antibiotic treatment and who delivered infants with lower gestational age and small for gestational age. ACS use was highest in the 28-31 weeks gestational age group, and lowest in the under 26 weeks of gestational age group (x = 65.478, P < 0.001). ACS exposure was associated with lower odds of bronchopulmonary dysplasia or death (OR, 0.778; 95% CI 0.661 to 0.916) and invasive respiration requirement (OR, 0.668; 95% CI 0.585 to 0.762) in this population.

Conclusion: The ACS exposure was variable among maternity hospitals and quality improvement of ACS administration is warranted.
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http://dx.doi.org/10.1186/s12887-022-03529-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347139PMC
August 2022

Aromatic disulfides as potential inhibitors against interaction between deaminase APOBEC3G and HIV infectivity factor.

Acta Biochim Biophys Sin (Shanghai) 2022 May;54(5):725-735

State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China.

APOBEC3G (A3G) is a member of cytosine deaminase family with a variety of innate immune functions. It displays activities against retrovirus and retrotransposon by inhibition of virus infectivity factor (Vif)-deficient HIV-1 replication. The interaction between A3G N-terminal domain and Vif directs the cellular Cullin 5 E3-ubiquitin ligase complex to ubiquitinate A3G, and leads to A3G proteasomal degradation, which is a potential target for anti-HIV drug. Currently, there are very few reports about stable small molecules targeting the interaction between A3G and Vif. In this study, we screened two series of small molecules containing carbamyl sulfamide bond or disulfide bond as bridges of two different aromatic rings. Five asymmetrical disulfides were successfully identified against interaction between A3G and Vif with the IC values close to or smaller than 1 μM, especially, not through covalently binding with A3G or Vif. They restore the A3G expression in the presence of Vif by inhibiting Vif-induced A3G ubiquitination and degradation. This study opens a way to the discovery of new anti-HIV drugs.
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http://dx.doi.org/10.3724/abbs.2022049DOI Listing
May 2022

Virome of Giant Panda-Infesting Ticks Reveals Novel Bunyaviruses and Other Viruses That Are Genetically Close to Those from Giant Pandas.

Microbiol Spectr 2022 Aug 2:e0203422. Epub 2022 Aug 2.

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.

Tick infestations have been reported as one of the factors threatening the health of giant pandas, but studies of viral pathogens carried by ticks feeding on the blood of giant pandas are limited. To assess whether blood-sucking ticks of giant pandas can carry viral pathogens and if so, whether the viruses in ticks are associated with those previously detected in giant panda hosts, we determined the viromes of ticks detached from giant pandas in a field stocking area in Sichuan Province, southwest China. Using viral metagenomics we identified 32 viral species in ticks, half of which (including anellovirus [ = 9], circovirus [ = 3], and gemycircularvirus [ = 4]) showed homology to viruses carried by giant pandas and their associated host species (such as red pandas and mosquitoes) in the same living domain. Remarkably, several viruses in this study phylogenetically assigned as bunyavirus, hepe-like virus, and circovirus were detected with relatively high abundance, but whether these newly identified tick-associated viruses can replicate in ticks and then transmit to host animals during a blood meal will require further investigation. These findings further expand our understanding of the role of giant panda-infesting ticks in the local ecosystem, especially related to viral acquisition and transmission, and lay a foundation to assess the risk for giant panda exposure to tick-borne viruses. Ticks rank only second to mosquitoes as blood-feeding arthropods, capable of spreading pathogens (including viruses, bacteria, and parasites) to hosts during a blood meal. To better understand the relationship between viruses carried by ticks and viruses that have been reported in giant pandas, it is necessary to analyze the viromes of giant panda-parasitic blood-sucking ticks. This study collected 421 ticks on the body surface of giant pandas in Sichuan Province, China. We characterized the extensive genetic diversity of viruses harbored by these ticks and reported frequent communication of viruses between giant pandas and their ticks. While most of the virome discovered here are nonpathogenic viruses from giant pandas and potentially tick-specific viruses, we revealed some possible tick-borne viruses, represented by novel bunyaviruses. This research contributes to the literature because currently there are few studies on the virome of giant panda-infesting ticks.
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http://dx.doi.org/10.1128/spectrum.02034-22DOI Listing
August 2022

Ultrafast Laser-Ablated Bioinspired Hydrogel-Based Porous Gating System for Sustained Drug Release.

ACS Appl Mater Interfaces 2022 Aug 1;14(31):35366-35375. Epub 2022 Aug 1.

Department of Clinical Laboratory, The First Affiliated Hospital of USTC Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230027, China.

Gating systems have been extensively researched in energy harvesting, lab-on-chip applications, and so forth. However, the controlled drug delivery system with artificial hydrogel-based porous gating systems (HPGSs) is rarely reported. Herein, a biomimetic HPGS with a pH-responsive hydrogel as the valve and polydimethylsiloxane as the frame is fabricated by in situ femtosecond laser microdrilling and subsequent ultraviolet exposure. The proposed HPGS loaded with doxorubicin hydrochloride (DOX) is stable under physiological conditions, has a low drug leakage rate, and can achieve sustained drug release in a low pH environment. The experimental results show that the drug release is mainly controlled by non-Fickian diffusion, which renders the dynamic speed control of molecular transport possible. Moreover, the HPGS can also be prepared into an antitumor microcapsule. The results of in vitro cell experiments demonstrate that [email protected] can release drugs and achieve terrific therapeutic efficacy in the elimination of HeLa cells in the acidic environments around tumor cells. This functional HPGS is envisioned to be an ideal pH-response carrier for sustained drug release treatment of digestive diseases such as inflammatory bowel disease and gastrointestinal cancer.
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http://dx.doi.org/10.1021/acsami.2c07319DOI Listing
August 2022

Assessing sediment organic pollution via machine learning models and resource performance.

Bioresour Technol 2022 Jul 26;361:127710. Epub 2022 Jul 26.

Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Sichuan 611130, China. Electronic address:

Due to the potential ecological risks of organic pollution in sediments, aquatic ecosystems are currently facing substantial environmental threats. Assessing and controlling sediment pollution has become a huge challenge. Therefore, this study proposes a novel strategy for predicting organic pollution indicators for sediment, as well as an effective resource-utilization method. Contaminated sediments were converted into catalysts for sulfate radical advanced oxidation technologies by a one-step calcination method. The results revealed that the catalyst excelled in activating peroxymonosulfate to degrade tetracycline via a non-radical pathway. Most importantly, a predictive model of organic pollution indicators was established by machine learning. This study provides a novel approach for resource utilization and a strategy for assessing organic pollution in sediments.
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http://dx.doi.org/10.1016/j.biortech.2022.127710DOI Listing
July 2022

Modulating the glycemic response of starch-based foods using organic nanomaterials: strategies and opportunities.

Crit Rev Food Sci Nutr 2022 Jul 28:1-25. Epub 2022 Jul 28.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, China.

Traditionally, diverse natural bioactive compounds (polyphenols, proteins, fatty acids, dietary fibers) are used as inhibitors of starch digestive enzymes for lowering glycemic index (GI) and preventing type 2 diabetes mellitus (T2DM). In recent years, organic nanomaterials (ONMs) have drawn a great attention because of their ability to overcome the stability and solubility issues of bioactive. This review aimed to elucidate the implications of ONMs in lowering GI and as encapsulating agents of enzymes inhibitors. The major ONMs are presented. The mechanisms underlying the inhibition of enzymes, the stability within the gastrointestinal tract (GIT) and safety of ONMs are also provided. As a result of encapsulation of bioactive in ONMs, a more pronounced inhibition of enzymes was observed compared to un-encapsulated bioactive. More importantly, the lower the size of ONMs, the higher their inhibitory effects due to facile binding with enzymes. Additionally, in vivo studies exhibited the potentiality of ONMs for protection and sustained release of insulin for GI management. Overall, regulating the GI using ONMs could be a safe, robust and viable alternative compared to synthetic drugs (acarbose and voglibose) and un-encapsulated bioactive. Future researches should prioritize ONMs in real food products and evaluate their safety on a case-by-case basis.
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http://dx.doi.org/10.1080/10408398.2022.2097638DOI Listing
July 2022

miR-4443/MMP2 suppresses the migration and invasion of trophoblasts through the HB-EGF/EGFR pathway in preeclampsia.

Cell Cycle 2022 Jul 28:1-16. Epub 2022 Jul 28.

International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Preeclampsia (PE) is a pregnancy-associated disease that may cause maternal and fetal morbidity and mortality. The dysregulation of microRNAs (miRNAs) and their potential functions has been an important direction for elucidating the mechanism of preeclampsia in recent years. The present study investigated whether miR-4443 was significantly increased in the placentas of severe preeclamptic patients, and the upregulation of miR-4443 inhibited the migration and invasion of HTR-8/SVneo cells according to transwell assays. Matrix metallopeptidase 2 (MMP2), which is involved in the degradation of extracellular matrix (ECM) components and harbors a miR-4443-binding site within its 3'-UTR as confirmed by a luciferase reporter assay, was identified to be directly inhibited by miR-4443. Moreover, siRNA targeting MMP2 imitated the effects of overexpressed miR-4443 on HTR-8/SVneo cell invasion and migration, whereas rescue experiments showed that MMP2 reversed this inhibitory function of miR-4443. Heparin-binding EGF-like growth factor (HB-EGF), as the downstream gene of MMP2, plays an important role in trophoblast invasion, and we confirmed that the expression of HB-EGF/EGFR pathway-related biomolecules was consistent with MMP2 influenced by upregulating and downregulating miR-4443 and that activated EGFR further transmitted intracellular downstream signaling via the MAPK pathway according to western blot assay. In conclusion, we demonstrated that miR-4443 suppresses the migration and invasion of trophoblasts, and its inhibitory effects are at least partially mediated by the suppression of MMP2. This inhibition might further affect the progression of preeclampsia through the HB-EGF/EGFR pathway, thus providing a new clue on the role of miR-4443 in the pathogenesis of preeclampsia.
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http://dx.doi.org/10.1080/15384101.2022.2103897DOI Listing
July 2022

Electrochemical collective synthesis of labeled pyrroloindoline alkaloids with Freon-type methanes as functional C1 synthons.

Chem Commun (Camb) 2022 Aug 16;58(66):9230-9233. Epub 2022 Aug 16.

Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs & School of Chemistry and Chemical Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China.

Utilization of Freon-type methanes as functional one-carbon synthons in the synthesis of various deuterated indoline alkaloids was demonstrated here. A series of halomethyl radicals were generated from electro-reductive C-X cleavage of Freon-type methanes and captured efficiently by acrylamides to provide various halogenated oxindoles radical cyclization. This reaction features good functional group tolerance, and deuterium and fluorine atoms could be introduced facilely from Freon-type methanes. Further transformation of halogenated oxindoles enabled the synthesis of many (labeled) bioactive drug molecules and skeletons, such as deuterated (±)-physostigmine, deuterated (±)-esermethole and deuterated (±)-lansai B.
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http://dx.doi.org/10.1039/d2cc03301aDOI Listing
August 2022

Learning-Based Slip Detection for Robotic Fruit Grasping and Manipulation under Leaf Interference.

Sensors (Basel) 2022 Jul 22;22(15). Epub 2022 Jul 22.

Laboratory of Motion Generation and Analysis, Faculty of Engineering, Monash University, Clayton, VIC 3800, Australia.

Robotic harvesting research has seen significant achievements in the past decade, with breakthroughs being made in machine vision, robot manipulation, autonomous navigation and mapping. However, the missing capability of obstacle handling during the grasping process has severely reduced harvest success rate and limited the overall performance of robotic harvesting. This work focuses on leaf interference caused slip detection and handling, where solutions to robotic grasping in an unstructured environment are proposed. Through analysis of the motion and force of fruit grasping under leaf interference, the connection between object slip caused by leaf interference and inadequate harvest performance is identified for the first time in the literature. A learning-based perception and manipulation method is proposed to detect slip that causes problematic grasps of objects, allowing the robot to implement timely reaction. Our results indicate that the proposed algorithm detects grasp slip with an accuracy of 94%. The proposed sensing-based manipulation demonstrated great potential in robotic fruit harvesting, and could be extended to other pick-place applications.
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http://dx.doi.org/10.3390/s22155483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332724PMC
July 2022

Preliminary Study on Gene Regulation and its Pathways in Chinese Holstein Cows with Clinical Mastitis Caused by .

J Vet Res 2022 Jun 5;66(2):179-187. Epub 2022 May 5.

College of Animal Science and Technology of Huazhong Agricultural University, Wuhan, PR China.

Introduction: Clinical mastitis (CM) is one of the most common diseases of dairy cows globally, has a complex aetiology and recurs easily. is a frequently isolated pathogen responsible for bovine mastitis and remains difficult to eradicate.

Material And Methods: To characterise the transcriptional profiles of dairy cows infected by , we performed an RNA-seq analysis of peripheral blood leukocytes in lactating Chinese Holstein dairy cows with CM and did the same with healthy cows' samples as controls.

Results: A total of 4,286 genes were detected in the CM cases infected with which were differentially expressed compared to the controls, 3,085 of which were upregulated, the remainder being downregulated. Notably, we observed that some differentially expressed genes (DEGs) had strong protein-protein interaction. Of these, six downregulated DEGs (, , , , , and ) were involved in the metabolic pathway, while eight upregulated DEGs (, , , , , , , and ) were associated with the platelet activation pathway.

Conclusion: The transcriptome dataset of CM cases would be a valuable resource for clinical guidance on anti-inflammatory medication and for deeper understanding of the biological processes of CM response to infection, and it would enable us to identify specific genes for diagnostic markers and possibly for targeted therapy.
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http://dx.doi.org/10.2478/jvetres-2022-0022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281521PMC
June 2022

Transmembrane Protein LMxysn_ of Serovar 4h Is Associated with Bile Salt Resistance and Intestinal Colonization.

Microorganisms 2022 Jun 21;10(7). Epub 2022 Jun 21.

Jiangsu Key Laboratory of Zoonosis, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, China.

(Lm) is a ubiquitous foodborne pathogen comprising of 14 serotypes, of which serovar 4h isolates belonging to hybrid sub-lineage Ⅱ exhibit hypervirulent features. LMxysn_ of serovar 4h Lm XYSN, a member of genomic island-7 (GI-7), is predicted to a membrane protein with unknown function, which is conserved in serovar 4h . Under bile salts stress, Lm XYSN strain lacking LMxysn_ (XYSN∆) exhibited a stationary phase growth defect as well as a reduction in biofilm formation and strikingly down-regulated bile-salts-resistant genes and virulent genes. Particularly, LMxysn_1693 protein plays a crucial role in Lm XYSN adhesion and invasion to intestinal epithelial cells, as well as colonization in the ileum of mice. Taken together, these findings indicate that the LMxysn_ gene encodes a component of the putative ABC transporter system, synthetically interacts with genes involved in bile resistance, biofilm formation and virulence, and thus contributes to survival within and outside the host.
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http://dx.doi.org/10.3390/microorganisms10071263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320622PMC
June 2022

Identification and Characterization of Species Associated with Plant Diseases in Southern China.

J Fungi (Basel) 2022 Jul 9;8(7). Epub 2022 Jul 9.

Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

species are important plant pathogens on a wide range of hosts, causing significant losses to plant production worldwide. During our survey on phytopathogenic fungi from 2019 to 2021, diseased samples were collected from various hosts in Guangdong Province, China. In total, 16 isolates were obtained from leaf spots, stem blights and root rots of species of , , , , , and plants. Isolates were identified morphologically, and a multigene phylogenetic analysis of combined partial sequences of calmodulin (A), translation elongation factor 1-alpha (1-α) and beta-tubulin () was performed. These sixteen isolates were further identified as nine species, with five new species: . , . &nbsp;. , . and . as well as four new records: Ca. from , Ca. from sp., . from , and . from . Moreover, we provide updated phylogenetic trees for four species complexes viz. . , . , . and . . Our study is the first comprehensive study on species associated with various hosts from subtropical regions in China. Results from the present study will be an addition to the biodiversity of microfungi in South China.
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http://dx.doi.org/10.3390/jof8070719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324520PMC
July 2022

Association of Maternal History of Preterm Birth With Congenital Anomalies in Offspring.

JAMA Netw Open 2022 07 1;5(7):e2223614. Epub 2022 Jul 1.

Department of Pediatric Cardiothoracic Surgery, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China.

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http://dx.doi.org/10.1001/jamanetworkopen.2022.23614DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315420PMC
July 2022

Development, characterisation, and in vitro anti-tumor effect of self-microemulsifying drug delivery system containing polyphyllin I.

Drug Deliv Transl Res 2022 Jul 25. Epub 2022 Jul 25.

Grade Three Laboratory of Traditional Chinese Medicine Preparation of the National Administration of Traditional Chinese Medicine, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250014, China.

Polyphyllin I (PPI), an effective active ingredient in Paris polyphylla, has a diverse set of pharmacological properties. However, due to its poor solubility and oral absorption, its application and development are limited. In the study, we were committed to improving the solubility of PPI by developing a self-microemulsifying drug delivery system of PPI (PPI-SMEDDS), screening the best preparation process, and evaluating the quality and the in vivo pharmacokinetics of PPI, and PPI-SMEDDS following oral administration to rats were also studied. In addition, the pharmacological activities against human lung adenocarcinoma cell A549 in vitro were assessed. The best formulation had 15.89% ethyl oleate, 47.38% Cremophor RH40, and 36.73% 1,2 propylene glycol. The produced PPI-SMEDDS was clear and transparent, with an average particle size of 24.51 nm and a zeta potential of -17.54 ± 0.51 mV. In vitro, the cumulative release rate of PPI-SMEDDS was nearly 80% within 2 h. PPI-SMEDDS had a substantially greater area under the curve than PPI following oral treatment in rats, and the relative bioavailability of PPI in rats was 278.99%. More importantly, the anti-tumor effect of PPI-SMEDDS in vitro was significantly greater than that of PPI. These findings suggested that PPI-SMEDDS has the potential to improve the solubility, oral bioavailability of PPI, and anti-tumor effect, laying the groundwork for future research on the new PPI dosage form.
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http://dx.doi.org/10.1007/s13346-022-01212-8DOI Listing
July 2022

Green and Large-Scale Preparation of Chiral Metal-Organic Frameworks via Mechanochemistry.

Inorg Chem 2022 Aug 25;61(31):12190-12196. Epub 2022 Jul 25.

Key laboratory of Jiangxi Province for Environment and Energy Catalysis, School of Chemistry and Chemical Engineering, Nanchang University, Nanchang 330031, P. R. China.

It is a significant challenge to construct chiral metal-organic frameworks (CMOFs) by developing a facile and green preparation strategy. In this work, CMOFs were first synthesized via a mechanochemical process by combining a truncated mixed ligand strategy and defect engineering theory. The simple, green, and rapid construction strategy could solvent-freely harvest gram-scale CMOFs with a hierarchical micro/mesoporous structure. The as-synthesized CMOFs were evaluated by Aldol asymmetric catalysis and exhibited excellent catalytic performance (conversion was up to 97.1%, the ee value was 44.3%, and the activity was still good after 5 cycles).
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http://dx.doi.org/10.1021/acs.inorgchem.2c01261DOI Listing
August 2022
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