Publications by authors named "Bushra Hamid"

12 Publications

  • Page 1 of 1

Optimized Biobanking Procedures for Preservation of RNA in Tissue: Comparison of Snap-Freezing and RNAlater-Fixation Methods.

Biopreserv Biobank 2019 Dec 16;17(6):562-569. Epub 2019 Oct 16.

Molecular Unit, Department of Pathology, Herlev and Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.

Personalized treatment, supported by biomarkers, would improve survival of ovarian cancer patients. RNA molecules are potentially important biomarkers. The Danish CancerBiobank provides an infrastructure for handling and storage of biological material, including RNA, from Danish cancer patients. The aim of this study was to investigate the effects of handling-time and fresh-freezing versus RNAlater fixation on RNA degradation in solid tissue from pelvic mass samples. We evaluated RNA quality in surgical tissue from patients with a pelvic mass. Corresponding samples were either fresh-frozen or fixed in RNAlater, at eight different time points after the surgery. Integrity was measured using a bioanalyzer, and the amount and quality were further investigated by quantitative reverse transcription-polymerase chain reaction measuring the expression of housekeeping genes and . Our results show that tissue RNA is stable up to at least 180 minutes after the surgery, as the quality was comparable to the quality of RNA handled immediately. Likewise, patient RNA was of acceptable quality after both fresh-frezing and RNAlater fixation, but RNAlater fixation was slightly more effective for RNA preservation. Our data suggest that RNA in pelvic mass samples is relatively stable. Knowledge about RNA stability is an important prerequisite for research in RNA biomarkers, where the challenge is to balance the need for careful RNA handling and storage with the need for effective large-scale biobanking in a busy clinical setting where patient treatment is the main priority.
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http://dx.doi.org/10.1089/bio.2019.0028DOI Listing
December 2019

Uretero-Arterial Fistula - Not So Rare?

Curr Urol 2018 Oct 30;12(1):54-56. Epub 2018 Jun 30.

Department of Urology, Countess of Chester Hospital, Chester, UK.

The first uretero-arterial fistula (UAF) was reported in 1908 by Moschcowitz. In 2009, a systematic review identified 139 cases. Since then a further 23 cases were described with 19 cases originating from a single center. It has been recognized as a very rare condition in the past. However, more recently, the increasing incidence of UAF has led us to believe that this condition is more frequent than previously described. Aging population, improved cancer survival and extensive multimodal pelvic cancer treatments have been recognized as culprits for the increased incidence of UAFs. We have reviewed literature on UAFs, identified risk factors, patho-physiology and treatment options. Also, we present a case of fistulous communication between the internal iliac artery and ureter in a patient with a potential risk factor previously not described in the literature.
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http://dx.doi.org/10.1159/000489419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198778PMC
October 2018

Incidental Mesenteric Paraganglioma: A Case Report and Literature Review.

Ann Coloproctol 2017 Oct 31;33(5):197-200. Epub 2017 Oct 31.

Colorectal Surgical Department, Countess of Chester Hospital NHS Foundation Trust, Countess of Chester Health Park, Chester, United Kingdom.

Mesenteric paragangliomas are a rare entity; consequently, only 12 cases have been reported to date. Although considered benign and often found incidentally, they have the potential to metastasize and are an important diagnosis to consider for patients with a mesenteric tumor. We report the case of a 71-year-old woman who was found on magnetic resonance imaging to have an incidental, large, complex, intra-abdominal mass that had initially been misdiagnosed as ovarian in origin. She underwent an open resection of the tumor and adjacent small bowel with no perioperative complications; histology confirmed the mass to be a mesenteric paraganglioma. This case report highlights several important key issues regarding paraganglioma, including diagnosis, imaging, genetic testing, and surgical resection. The results of a literature review are also discussed.
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http://dx.doi.org/10.3393/ac.2017.33.5.197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5683971PMC
October 2017

Inspection of Ureteral Orifices: The Pearl of Flexible Cystoscopy.

J Endourol Case Rep 2015 1;1(1):6-8. Epub 2015 Oct 1.

Countess of Chester Hospital NHS Foundation Trust, Chester, United Kingdom .

Cystoscopy is most common diagnostic investigation. The examination technique and the findings, both normal and pathological, were well described described a hundred years ago. With technological advances, there has been over-emphasis on imaging modalities for diagnostic purposes. A basic maneuver of examining the ureteral orifices is sometimes rushed through when in fact careful examination can clinch the diagnosis. The importance is exemplified by two cases, one of which is a rare case of Xanthoma of the ureter.
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http://dx.doi.org/10.1089/cren.2015.29011.smiDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996568PMC
August 2016

Random conjunctival biopsy in multiorgan disease.

Br J Ophthalmol 2014 May 5;98(5):705, 714. Epub 2014 Mar 5.

Department of Ophthalmology, Countess of Chester Hospital, Chester, UK.

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http://dx.doi.org/10.1136/bjophthalmol-2013-304718DOI Listing
May 2014

Context is everything: an unusual breast core biopsy case.

Int J Surg Pathol 2013 Oct 11;21(5):502-3. Epub 2013 Jul 11.

1Countess of Chester Hospital, Chester, Cheshire, UK.

Changes in breast tissue in female-to-male transsexuals following gender reassignment and androgen therapy can cause difficulties in interpreting breast core biopsies. Clinical history and awareness of histological changes in breast tissue associated with androgen treatment are important in such cases. Specimen mislabeling is a potential pitfall to be borne in mind while evaluating unusual presentations in breast core biopsies. We report a case of a 58-year-old male with a well-defined supra areolar lesion clinically thought to be a fibroadenoma.
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http://dx.doi.org/10.1177/1066896913496149DOI Listing
October 2013

Colocutaneous fistula secondary to amoebiasis.

Int J Surg Case Rep 2011 21;2(3):40-3. Epub 2010 Dec 21.

Countess of Chester Hospital NHS Foundation Trust, Countess of Chester Health Park, Liverpool Road, Chester, Cheshire CH2 1UL, UK.

Here we present an interesting and extremely rare case of a 66 year old male who developed a colocutaneous fistula secondary to amoebiasis. The patient presented with an acute history of right lower abdominal pain, weight loss and a palpable mass. A CT scan demonstrated a fluid filled cavity in the right iliac fossa consistent with an appendiceal abscess which was drained under radiological guidance. However, following drainage his symptoms remained requiring open surgical drainage, and a controlled caecostomy was performed due to a small caecal perforation. Despite appropriate conservative therapy he failed to progress, and developed localised sepsis in the right iliac fossa with a colocutaneous fistula, requiring a formal right hemicolectomy. The histological examination confirmed the presence of abundant trophozoites of Entamoeba histolytica.We highlight the fact that in the modern age of immigration and long distance travel, it will become increasingly likely that the so-called 'tropical' diseases will present throughout the world. This case also highlights the need to keep an open mind in cases that do not progress as expected, and to react accordingly to any unusual developments.
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http://dx.doi.org/10.1016/j.ijscr.2010.12.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3199675PMC
November 2011

Renal angiomyoadenomatoid tumor.

Urology 2011 Aug 4;78(2):327-8. Epub 2010 Dec 4.

Department of Urology, Countess of Chester Foundation NHS Foundation Trust, Cheshire, United Kingdom.

Renal angiomyoadenomatoid tumor is a distinct pathologic entity that can mimic clear cell renal adenocarcinoma in presentation. The nature and behavior of these tumors are not well understood, and they require long-term follow-up to clarify their neoplastic potential.
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http://dx.doi.org/10.1016/j.urology.2010.08.033DOI Listing
August 2011

Multiple periocular adult onset xanthogranulomas in a patient with chronic lymphocytic leukaemia.

Eur J Dermatol 2010 Mar-Apr;20(2):211-3. Epub 2009 Nov 17.

Department of Pathology, University Hospital of North Staffordshire, Stoke on Trent, UK.

Adult and juvenile forms of cutaneous xanthogranuloma share many clinical and histopathological features. Unlike the juvenile variant, systemic disorders including haematological malignancies have only rarely been reported in association with adult xanthogranuloma. We report a case of multiple periocular xanthogranulomas arising in a 43 year old man with chronic lymphocytic leukaemia. This case supports the emerging hypothesis that both juvenile and adult xanthogranulomas may share an association with an underlying haematological malignancy. Also highlighted is the fact that some patients with periocular cutaneous xanthogranulomas of adult onset can have co-existing orbital xanthogranulomatous disease and appropriate clinical and radiological investigations may be needed for accurate diagnosis.
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http://dx.doi.org/10.1684/ejd.2010.0858DOI Listing
June 2010

Appendiceal carcinoma masquerading as recurrent urinary tract infections: case report and review of literature.

Urology 2006 Aug;68(2):428.e1-3

Countess of Chester Hospital NHS Foundation Trust, Liverpool, United Kingdom.

We report on a female patient who presented with recurrent urinary tract infections. The investigations revealed an adenocarcinoma of the appendix fistulating into the bladder and causing irritative symptoms. After right hemicolectomy and partial cystectomy, she was well at 3 months without any need for adjuvant therapy. This unusual situation reminds us that the finding of bladder adenocarcinoma is rare, and it would be prudent to examine the lower gastrointestinal tract thoroughly to look for a bowel source, before recommending any ablative surgery.
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http://dx.doi.org/10.1016/j.urology.2006.03.002DOI Listing
August 2006

Localized traumatic infarction of the testicle.

Scand J Urol Nephrol 2004 ;38(5):442-3

Department of Urology, Countess of Chester Hospital, Chester, UK.

Ultrasonically it is extremely difficult to accurately distinguish testicular infarct from neoplasm. We describe a case which highlights this, together with the previously unreported phenomenon of a localized infarct secondary to trauma.
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http://dx.doi.org/10.1080/00365590410028872DOI Listing
April 2005

Pelvic malignant hemangiopericytoma mimicking an ovarian neoplasm; a case report.

J Reprod Med 2004 May;49(5):404-7

Department of Obstetrics and Gynaecology, Countess of Chester Hospital, Liverpool Road, Chester, UK.

Background: Malignant hemangiopericytoma (MHPC) is a rare vascular tumor and has been reported to occur in the musculature of the extremities, retroperitoneum and pelvis. Omental hemangiopericytomas (HPCs) are extremely rare. Synovial sarcomas and solitary fibrous tumors share histologic features with HPCs, causing diagnostic difficulties. Immunohistochemistry is essential for the diagnosis.

Case: A 74-year-old woman presented with an abdominopelvic mass. A malignant ovarian tumor was suspected on clinical features, ultrasound and computed tomography. Staging laparotomy revealed a large, vascular tumor adherent to loops of small bowel, colon, cecum and appendix, but the ovaries and uterus were normal. The tumor was completely removed after extensive dissection. Histopathology and detailed immunohistochemistry established the diagnosis of a malignant hemangiopericytoma arising from the omentum. The patient developed recurrent subacute bowel obstruction and died 4 months after the initial diagnosis.

Conclusion: MHPCs are rare tumors and not likely to be diagnosed preoperatively. Treatment is therefore individualized and based on the findings at laparotomy. Some tumors, such as the one described here, exhibit very aggressive behavior.
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May 2004
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