Publications by authors named "Bo-Yun Kim"

16 Publications

  • Page 1 of 1

The complete plastid genome sequence of (Fagaceae), an evergreen broad-leaved oak endemic to East Asia.

Mitochondrial DNA B Resour 2021 Feb 3;6(2):320-322. Epub 2021 Feb 3.

Department of Biology Education, Chonnam National University, Gwangju, Republic of Korea.

We are reporting the complete plastid genome (plastome) of , an evergreen broad-leaved oak endemic to East Asia. This species is important for maintaining the warm-temperate evergreen forest biome in East Asia. The plastome is 160,522 base pairs (bp) long, with two inverted repeat (IR) regions (25,839 bp each) that separate a large single copy (LSC) region (90,199 bp) and a small single copy (SSC) region (18,645 bp). The phylogenetic tree shows that is closely related to with strong bootstrap support.
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http://dx.doi.org/10.1080/23802359.2020.1866449DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872549PMC
February 2021

The complete chloroplast genome of Nakai from Dokdo Island in Korea (Campanulaceae).

Mitochondrial DNA B Resour 2021 Jan 13;6(1):135-137. Epub 2021 Jan 13.

Department of Biology, Daejeon University, Daejeon, Korea.

Completed chloroplast genome of Nakai isolated from Dokdo island in Korea is 169,719 bp long (GC ratio is 38.8%) and has four subregions: 102,381 bp of large single-copy (37.8%) and 7,750 bp of small single-copy (32.6%) regions are separated by 29,794 bp of inverted repeat (41.3%) regions including 131 genes (87 protein-coding genes, eight rRNAs, and 36 tRNAs). Phylogenetic analyses suggested that . from Dokdo Island form a clade with . from Ulleungdo Island and that chloroplast genomes of the two accessions are diverged.
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http://dx.doi.org/10.1080/23802359.2020.1851157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819127PMC
January 2021

A Disjunctive Marginal Edge of Evergreen Broad-Leaved Oak () in East Asia: The High Genetic Distinctiveness and Unusual Diversity of Jeju Island Populations and Insight into a Massive, Independent Postglacial Colonization.

Genes (Basel) 2020 Sep 23;11(10). Epub 2020 Sep 23.

Department of Biology Education, Chonnam National University, Gwangju 61186, Korea.

Jeju Island is located at a marginal edge of the distributional range of East Asian evergreen broad-leaved forests. The low genetic diversity of such edge populations is predicted to have resulted from genetic drift and reduced gene flow when compared to core populations. To test this hypothesis, we examined the levels of genetic diversity of marginal-edge populations of , restricted to a few habitats on Jeju Island, and compared them with the southern Kyushu populations. We also evaluated their evolutionary potential and conservation value. The genetic diversity and structure were analyzed using 40 polymorphic microsatellite markers developed in this study. Ecological Niche Modeling (ENM) has been employed to develop our insights, which can be inferred from historical distribution changes. Contrary to our expectations, we detected a similar level of genetic diversity in the Jeju populations, comparable to that of the southern Kyushu populations, which have been regarded as long-term glacial refugia with a high genetic variability of East Asian evergreen trees. We found no signatures of recent bottlenecks in the Jeju populations. The results of STRUCTURE, neighbor-joining phylogeny, and Principal Coordinate Analysis (PCoA) with a significant barrier clearly demonstrated that the Jeju and Kyushu regions are genetically distinct. However, ENM showed that the probability value for the distribution of the trees on Jeju Island during the Last Glacial Maximum (LGM) converge was zero. In consideration of these results, we hypothesize that independent massive postglacial colonization from a separate large genetic source, other than Kyushu, could have led to the current genetic diversity of Jeju Island. Therefore, we suggest that the Jeju populations deserve to be separately managed and designated as a level of management unit (MU). These findings improve our understanding of the paleovegetation of East Asian evergreen forests, and the microevolution of oaks.
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http://dx.doi.org/10.3390/genes11101114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598624PMC
September 2020

An unexpected genetic diversity pattern and a complex demographic history of a rare medicinal herb, Chinese asparagus (Asparagus cochinchinensis) in Korea.

Sci Rep 2019 07 5;9(1):9757. Epub 2019 Jul 5.

Department of Life Sciences, Hallym University, Chuncheon, 24252, South Korea.

Range-wide population studies of wide spread species are often associated with complex diversity patterns resulting from genetically divergent evolutionary significant units (ESUs). The compound evolutionary history creating such a pattern of diversity can be inferred through molecular analyses. Asparagus cochinchinensis, a medicinally important perennial herb, is in decline due to overharvesting in Korea. Eight A. cochinchinensis populations in Korea and three populations from neighboring countries (China, Japan and Taiwan) were examined using nine nuclear microsatellite loci and three chloroplast microsatellite loci to characterize molecular diversity patterns. The average within-population diversity was limited likely due to long-term bottlenecks observed in all eight populations. High pairwise F values indicated that the populations have largely diverged, but the divergences were not correlated with geographic distances. Clustering analyses revealed a highly complex spatial structure pattern associated with two ESUs. Approximate Bayesian Computation (ABC) suggests that the two ESUs split about 21,000 BP were independently introduced to Korea approximately 1,800 years ago, and admixed in secondary contact zones. The two ESUs found in our study may have different habitat preferences and growth conditions, implying that the two genetically divergent groups should be considered not only for conservation and management but also for breeding programs in agricultural areas.
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http://dx.doi.org/10.1038/s41598-019-46275-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611897PMC
July 2019

Development and characterization of 30 microsatellite loci for (Berberidaceae).

Appl Plant Sci 2018 Dec 4;6(12):e01200. Epub 2018 Dec 4.

Department of Life Science Hallym University Chuncheon 24252 Republic of Korea.

Premise Of The Study: (Berberidaceae) has been listed as an endangered species in Korea due to extensive collection and destruction of natural habitats. In this study, 30 microsatellite loci, including 25 polymorphic loci, were developed for for use in population-level genetic analyses.

Methods And Results: We carried out transcriptome sequencing and isolated a total of 30 expressed sequence tag-simple sequence repeat markers from using Illumina HiSeq high-throughput sequencing. To test utility of the developed markers, we genotyped 60 individuals from three populations and estimated the number of alleles and levels of observed and expected heterozygosity. Expected heterozygosity levels ranged from 0.000 to 0.594, 0.000 to 1.000, and 0.000 to 0.744 in the three populations, respectively.

Conclusions: These transcriptome-derived simple sequence repeat markers are highly polymorphic and can be widely used in characterization of the endangered . Population genetic studies with these markers will provide valuable insights for conservation by unraveling evolutionary patterns of .
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http://dx.doi.org/10.1002/aps3.1200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303150PMC
December 2018

Genetic diagnosis of a rare myrmecochorous species, (Berberidaceae): Historical genetic bottlenecks and strong spatial structures among populations.

Ecol Evol 2018 Sep 7;8(17):8791-8802. Epub 2018 Aug 7.

Department of Life Sciences Hallym University Chuncheon-si Korea.

Distribution of genetic variation over time and space is relevant to demographic histories and tightly linked to ecological disturbances as well as evolutionary potential of an organism. Therefore, understanding the pattern of genetic diversity is a primary step in conservation and management projects for rare and threatened plant species. We used eight microsatellite markers to examine the level of genetic diversity, spatial structure, and demographic history of , a rare myrmecochorous herb, populations sampled across northeast Asia and Siberia. We found low within-population genetic variation associated with historical bottlenecks. Although pairwise values were not much higher than the ones found in similar life form species, STRUCTURE and PCoA revealed a clear broadscale spatial pattern of genetic structure. Bayesian clustering (best  = 6) and PCoA identified three populations that are distinctive from neighboring populations in the Korean peninsula, which suggests potential units for conservation and management plans in Korea. MIGRATE-N and BAYESASS showed that both contemporary (0.003-0.045) and historical migration rates (2 × e-4.6 × e) were low. Our findings provide a good example, where genetic considerations should be integrated for conservation and management plans of rare and threatened species.
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http://dx.doi.org/10.1002/ece3.4362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157670PMC
September 2018

(Balsaminaceae), a new species from Cambodia.

PhytoKeys 2017 7(77):33-39. Epub 2017 Feb 7.

Department of Life Science, Hallym University, 1 Hallymdaehak-gil, Chuncheon-si, Gangwon 24252, Republic of Korea.

, a new species of family Balsaminaceae from Phnum Bokor National Park in southwestern Cambodia, is described and illustrated. The species is similar to , but is readily distinguished by the orbicular-obovate dorsal petal, shorter pedicels and larger seeds.
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http://dx.doi.org/10.3897/phytokeys.77.11345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5558809PMC
February 2017

Development of microsatellite markers based on expressed sequence tags in (Asparagaceae).

Appl Plant Sci 2017 Apr 18;5(4). Epub 2017 Apr 18.

Department of Life Science, Hallym University, Chuncheon 24252, Republic of Korea.

Premise Of The Study: Transcriptome-derived simple sequence repeat (SSR) markers were developed in (Asparagaceae). Due to its application in traditional medicine, its wild populations are threatened by over-collection even in protected areas, requiring immediate conservation efforts.

Methods And Results: Based on transcriptome data of , 96 primer pairs with two to seven alleles per locus were selected for initial validation; of those, 27 primer pairs amplified across all samples, resulting in 15 polymorphic and 12 monomorphic microsatellite markers. The usefulness of these markers was assessed in 60 individuals representing three populations of . Observed and expected heterozygosity values ranged from 0.050 to 0.950 and 0.049 to 0.626, respectively. Cross-species amplification of the 27 markers was tested in the related species . and .

Conclusions: These polymorphic, transcriptome-derived SSR markers can be used as molecular markers to study population genetics and ecological conservation in and related taxa.
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http://dx.doi.org/10.3732/apps.1700021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5400436PMC
April 2017

Development of microsatellite markers for (Santalaceae) and their application to wild populations.

Appl Plant Sci 2017 Jan 5;5(1). Epub 2017 Jan 5.

Department of Life Science, Hallym University, Chuncheon 24252, Republic of Korea.

Premise Of The Study: Microsatellite primers were developed for (Santalaceae), a semiparasitic medicinal plant that is known for its anticancer properties. Due to excessive human harvesting and loss of suitable habitat of its populations, it has become a potentially threatened species requiring immediate conservation efforts.

Methods And Results: Based on transcriptome data for , 124 primer pairs were randomly selected for initial validation, of which 19 yielded polymorphic microsatellite loci, with two to six alleles per locus. The usefulness of these markers was assessed for 60 individuals representing three populations of . Observed and expected heterozygosity values ranged from 0.033 to 0.833 and 0.032 to 0.672, respectively. Cross-species amplification for 19 loci in the related species was conducted.

Conclusions: The 19 newly developed loci are expected to be useful for studying the population genetics and ecological conservation of .
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http://dx.doi.org/10.3732/apps.1600102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5231913PMC
January 2017

Synthesis and Characterization of Water-Soluble Conjugated Oligoelectrolytes for Near-Infrared Fluorescence Biological Imaging.

ACS Appl Mater Interfaces 2016 Jun 16;8(25):15937-47. Epub 2016 Jun 16.

Department of Chemistry, Korea University , Seoul 02841, Republic of Korea.

Near-infrared (NIR) fluorophores attract increasing attention as a molecular marker (or probe) for in vivo and in vitro biological fluorescence imaging. Three types of new NIR fluorescent conjugated oligoelectrolytes (COEs: Q-FlTBTTFl, Q-FlBBTFl, and Q-FlTBBTTFl) are synthesized with quaternized ammonium ionic groups in their side-chains for water solubility. The emission wavelength is modulated in the range 600-1300 nm, by adjusting the intramolecular charge transfer in the molecular backbone based on the electron-rich fluorene (and/or thiophene) and electron-deficient benzo[2,1,3]thiadiazole (or benzo[1,2-c:4,5-c']bis[1,2,5]thiadiazole) moieties. The COEs show a remarkably larger Stokes shift (147-276 nm) compared to commercial rhodamine and cyanine dyes in water, avoiding self-quenching and interference from the excitation backscattered light. The photoluminescence (PL) quantum efficiency is improved substantially by up to 27.8% in water by fabricating a vesicular complex, COE/v, with a block ionomer, poly[(ethylene oxide)-block-(sodium 2-acrylamido-2-methyl-1-propanesulfonate)]. In vitro cellular uptake images with the COEs are obtained with good biocompatibility by confocal single-photon and two-photon microscopy. The ex vivo and in vivo images of a mouse xenograft model treated with the Q-FlBBTFl/v exhibit a substantially stronger fluorescence signal at the tumor site than at the other organs, highlighting the potential of the COE/v as an NIR fluorescent imaging agent for the diagnosis of cancer.
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http://dx.doi.org/10.1021/acsami.6b04276DOI Listing
June 2016

Magnetic self-assembly of gold nanoparticle chains using dipolar core-shell colloids.

Chem Commun (Camb) 2011 Jan 12;47(3):890-2. Epub 2010 Nov 12.

Department of Chemistry, University of Arizona, 1306 E. University Blvd., Tucson, AZ 85721, USA.

The preparation of gold nanoparticle (AuNP) assemblies was conducted by the synthesis and dipolar assembly of ferromagnetic core-shell nanoparticles composed of AuNP cores and cobalt NP shells. Dissolution of metallic Co phases with mineral acids afforded self-assembled AuNP chains and bracelets.
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http://dx.doi.org/10.1039/c0cc02375bDOI Listing
January 2011

Synthesis and colloidal polymerization of ferromagnetic Au-Co nanoparticles into Au-Co3O4 nanowires.

J Am Chem Soc 2010 Mar;132(10):3234-5

Department of Chemistry, University of Arizona, 1306 East University Boulevard, Tucson, Arizona 85752, USA.

The preparation of cobalt oxide nanowires with gold nanoparticle (AuNP) inclusions (Au-Co(3)O(4) nanowires) via colloidal polymerization of dipolar core-shell NPs is reported. Polystyrene-coated ferromagnetic NPs composed of a dipolar metallic cobalt shell and a gold NP core (PS-AuCoNPs) were synthesized by thermolysis of octacarbonyldicobalt [Co(2)(CO)(8)] in the presence of AuNP seeds and polymeric ligands. The colloidal polymerization process of these dipolar PS-AuCoNPs comprises dipolar nanoparticle assembly and solution oxidation of preorganized NPs to form interconnected cobalt oxide nanowires via the nanoscale Kirkendall effect, with AuNP inclusions in every repeating unit of the one-dimensional mesostructure. Calcination of the polymer-coated nanowires afforded polycrystalline Au-Co(3)O(4) nanowires that were determined to be electroactive. Nanocomposite materials were characterized by transmission electron microscopy, field-emission scanning electron microscopy, X-ray diffraction, vibrating sample magnetometry, and cyclic voltammetry. We demonstrate that the optical and electrochemical properties of Au-Co(3)O(4) nanowires are significantly enhanced in comparison with hollow Co(3)O(4) nanowires prepared via colloidal polymerization.
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http://dx.doi.org/10.1021/ja908481zDOI Listing
March 2010

Ferrocene functional polymer brushes on indium tin oxide via surface-initiated atom transfer radical polymerization.

Langmuir 2010 Feb;26(3):2083-92

Department of Chemistry, University of Arizona, 1306 E. University Boulevard, Tucson, Arizona 85721, USA.

The synthesis and electrochemical characterization of ferrocene functional polymethacrylate brushes on indium tin oxide (ITO) electrodes using surface-initiated atom transfer radical polymerization (SI-ATRP) is reported. SI-ATRP of ferrocene-containing methacrylate (FcMA) monomers from a phosphonic acid initiator-modified ITO substrate yielded well-defined homo- and block (co)polymer brushes of varying molar mass (4,000 to 37,000 g/mol). Correlation of both electrochemical properties and brush thicknesses confirmed controlled SI-ATRP from modified ITO surfaces. The preparation of block copolymer brushes with varying sequences of FcMA segments was conducted to interrogate the effects of spacing from the ITO electrode surface on the electrochemical properties of a tethered electroactive film.
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http://dx.doi.org/10.1021/la902590uDOI Listing
February 2010

Colloidal polymerization of polymer-coated ferromagnetic nanoparticles into cobalt oxide nanowires.

ACS Nano 2009 Oct;3(10):3143-57

Department of Chemistry, University of Arizona, Tucson, Arizona 85721, USA.

The preparation of polystyrene-coated cobalt oxide nanowires is reported via the colloidal polymerization of polymer-coated ferromagnetic cobalt nanoparticles (PS-CoNPs). Using a combination of dipolar nanoparticle assembly and a solution oxidation of preorganized metallic colloids, interconnected nanoparticles of cobalt oxide spanning micrometers in length were prepared. The colloidal polymerization of PS-CoNPs into cobalt oxide (CoO and Co(3)O(4)) nanowires was achieved by bubbling O(2) into PS-CoNP dispersions in 1,2-dichlorobenzene at 175 degrees C. Calcination of thin films of PS-coated cobalt oxide nanowires afforded Co(3)O(4) metal oxide materials. Transmission electron microscopy (TEM) revealed the formation of interconnected nanoparticles of cobalt oxide with hollow inclusions, arising from a combination of dipolar assembly of PS-CoNPs and the nanoscale Kirkendall effect in the oxidation reaction. Using a wide range of spectroscopic and electrochemical characterization techniques, we demonstrate that cobalt oxide nanowires prepared via this novel methodology were electroactive with potential applications as nanostructured electrodes for energy storage.
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http://dx.doi.org/10.1021/nn900483wDOI Listing
October 2009

Synthesis of Ru(II) complexes of N-heterocyclic carbenes and their promising photoluminescence properties in water.

Inorg Chem 2004 Nov;43(22):6896-8

School of Chemistry and Center for Molecular Catalysis, Seoul National University, Seoul 151-747, Korea.

Novel complexes 1 and 2 based on N-heterocyclic carbenes, which are analogous to Ru(bpy)(3)(2+) and Ru(terpy)(2)(2+), respectively, were synthesized. The complex, which is analogous to Ru(terpy)(2)(2+), exhibited promising photoluminescence properties with a long lifetime of 820 ns in acetonitrile and 3100 ns in water at room temperature, respectively. In addition, ab initio calculations were carried out.
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http://dx.doi.org/10.1021/ic049514fDOI Listing
November 2004

Unusual flexibility of 2,5-bis(4-pyridylethynyl)thiophene self-assembled with Co(NCS)2 in a novel coordination polymer.

Chem Commun (Camb) 2003 Oct(20):2528-9

School of Chemistry and Center for Molecular Catalysis, Seoul National University, Seoul 151-747, Korea.

A novel coordination polymer containing Co(NCS)2 and a rigid ligand, 2,5-bis(4-pyridylethynyl)-thiophene showing unusual flexibility was synthesized.
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http://dx.doi.org/10.1039/b307392kDOI Listing
October 2003