Publications by authors named "Bo Bai"

195 Publications

Cyclic enrichment of chromium based on valence state transformation in metal-free photocatalytic reductive imprinted composite hydrogel.

Sci Total Environ 2022 Sep 30;839:156367. Epub 2022 May 30.

School of Water and Environment, Chang'an University, Xi'an, Shaanxi 710054, PR China; Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region, Ministry of Education, Xi'an 710054, China.

Cr (VI) exists in anion form and can be reduced to positive charged Cr (III) under certain conditions. Can positive charged Cr (III) be continually used for absorbing Cr (VI) to achieve cyclic accumulation of chromium? In this paper, an ion imprinting material for adsorption of Cr (VI) was prepared by dispersing polypyrrole (PPy) in a gelatin/chitosan (Gel/CS) hydrogel network, named Gel/CS/PPy. Based on the conversion of Cr (VI) to Cr (III), a cyclic enrichment process including adsorption-photoreduction-fixation-readsorption of Cr (VI) was established in Gel/CS/PPy hydrogel. The composition and structure of the Gel/CS/PPy were analyzed by scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), thermogravimetric (TGA), texture analyzer (Universal TA), zeta potential and ultraviolet-visible-near infrared spectra (UV-vis-NIR). The conversion of Cr (VI) and Cr (III) and its promoting effect on readsorption were verified by XPS. The results showed that Gel/CS/PPy has good adsorption capacity for Cr (VI) and excellent photocatalytic ability to reduce Cr (VI) to Cr (III). Cr (III)-loaded Gel/CS/PPy can be further used to adsorb Cr (VI) and showed good adsorption efficiency even after four cycles. The optimal operating condition for Cr (VI) adsorption is pH = 3; 2 g/L dose of Gel/CS/PPy; and the adsorption capacity of Cr (VI) was about 106.8 mg/g after six adsorption cycles. Since Gel/CS/PPy is composed of organic components, high purity chromium can be recovered by simple calcination method later. Therefore, the synthesized Gel/CS/PPy has great potential in the practical application of low concentration Cr (VI) treatment in water.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156367DOI Listing
September 2022

Novel fabrication of hydrophobic/oleophilic human hair fiber for efficient oil/water separation through one-pot dip-coating synthesis route.

Sci Rep 2022 05 10;12(1):7632. Epub 2022 May 10.

Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region of the Ministry of Education, Chang'an University, No. 126 Yanta Road, Xi'an, 710054, Shanxi, China.

Frequent oil spill accidents and industrial wastewater discharge has always been one of the most severe worldwide environmental problems. To cope with this problem, many fluorine-containing and high-cost materials with superwettability have been extensively applied for oil-water separation, which hinders its large-scale application. In this work, a novel human hair fiber (HHF)-polymerized octadecylsiloxane (PODS) fiber was fabricated with a facile one-pot dip-coating synthesis approach, inspired by the self-assembly performance and hydrophobicity of OTS modification. The benefits of prominent hydrophobic/lipophilic behavior lie in the low surface energy, and a rough PODS coating was rationally adhered on the surface of HHF. Driven solely by gravity and capillary force, the HHF-PODS showed excellent oil/water separation efficiency (> 99.0%) for a wide range of heavy and light oil/water mixtures. In addition, HHF-PODS demonstrated durability toward different harsh environments like alkaline, acid, and salty solutions.
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http://dx.doi.org/10.1038/s41598-022-11511-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090757PMC
May 2022

Novel fabrication of a yeast biochar-based photothermal-responsive platform for controlled imidacloprid release.

RSC Adv 2021 May 28;11(32):19395-19405. Epub 2021 May 28.

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences Xining 810008 China.

For improving the utilization efficiency of pesticides, we developed a novel pesticide delivery particle ([email protected]) with a core-shell structure based on yeast biochar, imidacloprid (IMI), ammonium bicarbonate (NHHCO), calcium alginate (CA), and ethyl cellulose (EC). Therein, yeast biochar, IMI and NHHCO were absorbed in the network-structured of CA to obtain YINCP through hydrogen bonds. The resulting composite was granulated using an ion gelation technique and then coated with EC to form [email protected] In this platform, yeast biochar serving as a photothermal agent can efficiently convert sunlight energy into thermal energy, thereby triggering NHHCO decomposition into CO and NH that can break through the EC coating and facilitate IMI release. In addition, the influence of yeast biochar content, pH, and coexisting ions was systematically studied to evaluate the release behavior of IMI from [email protected] Moreover, the hydrophobic EC shell endowed [email protected] with high stability in aqueous solution for at least 60 days. Consequently, this novel composite with simple preparation, low cost and remarkable photothermal-responsive properties has a huge application potential in agriculture.
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http://dx.doi.org/10.1039/d1ra02143eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033562PMC
May 2021

Leaf (MIL) as a Novel Material in Atmospheric Water Collection.

ACS Omega 2022 Apr 28;7(14):11809-11817. Epub 2022 Mar 28.

Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region of the Ministry of Education, Changan University, Xi'an 710054, Shaanxi, China.

Here, leaves (MILs) have been used to collect atmospheric water for the first time. This novel material has been viewed by mankind as environmental waste and is mostly discarded or incinerated, causing environmental pollution. By turning waste into wealth, MILs have proven resourceful and can help ameliorate the water crisis, especially in tropical countries. The unprecedented water collection result is enough to describe MILs as an ideal material for atmospheric water collection when compared to other natural plants. Both the physical and chemical surface morphologies were extensively characterized. This comparative study shows that MIL surface droplet termination and hydrophilic nature differ from those of other materials, with the apex playing a key role in the roll-off of the droplet. The surface wettability and its interaction with the droplet are of keen interest in this study.
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http://dx.doi.org/10.1021/acsomega.1c07133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016854PMC
April 2022

[Research progress of different cell seeding densities and cell ratios in cartilage tissue engineering].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2022 Apr;36(4):470-478

Department of Orthopedics, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Guangdong, 510120, P. R. China.

Objective: To review the research progress of different cell seeding densities and cell ratios in cartilage tissue engineering.

Methods: The literature about tissue engineered cartilage constructed with three-dimensional scaffold was extensively reviewed, and the seeding densities and ratios of most commonly used seed cells were summarized.

Results: Articular chondrocytes (ACHs) and bone marrow mesenchymal stem cells (BMSCs) are the most commonly used seed cells, and they can induce hyaline cartilage formation and . Cell seeding density and cell ratio both play important roles in cartilage formation. Tissue engineered cartilage with good quality can be produced when the cell seeding density of ACHs or BMSCs reaches or exceeds that in normal articular cartilage. Under the same culture conditions, the ability of pure BMSCs to build hyaline cartilage is weeker than that of pure ACHs or co-culture of both.

Conclusion: Due to the effect of scaffold materials, growth factors, and cell passages, optimal cell seeding density and cell ratio need further study.
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http://dx.doi.org/10.7507/1002-1892.202110091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011064PMC
April 2022

Biotemplate Fabrication of Hollow Tubular CeSrTiO with Regulable Surface Acidity and Oxygen Mobility for Efficient Destruction of Chlorobenzene: Intrinsic Synergy Effect and Reaction Mechanism.

Environ Sci Technol 2022 May 23;56(9):5796-5807. Epub 2022 Mar 23.

State Key Laboratory of Multiphase Flow in Power Engineering, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, P. R. China.

Developing economic and applicable catalysts with elegant chlorine resistance and organic byproduct inhibition capability is of great significance for chlorinated volatile organic compounds (Cl-VOCs) eco-friendly purification. Here, ternary CeSrTiO catalysts with tunable surface acidity and oxygen species mobility were creatively fabricated using the hollow tubular-structured fruit hair of Platanus (FHP; a widespread greenery waste) as the scaffolding biotemplate. It is shown that the oxygen vacancy (O) triggered by the presence of Ce can optimize the synergy between the Lewis acid sites (LAS) and Brønsted acid sites (BAS). High concentration of O and BAS promotes the C-Cl cleavage of chlorobenzene (CB) and accelerates the desorption of Cl radicals as inorganic chlorine. Simultaneously, the strong electron transfer within Ti-Ce-Sr linkage increases the acidity of LAS, resulting in the superior reducibility of CeSrTiO and facilitating the deep oxidation of dechlorination intermediates. Additionally, the spatial confinement of the tubular structure remarkably accelerates the CB flow rate and reduces the residence time of byproducts over the prepared catalysts. Owing to these, CB can be efficiently destructed over CeSrTiO with selectivity of CO and inorganic chlorine dramatically enhanced, respectively, approximately 16 and 21 times at 275 °C compared to those of pure SrTiO. The present work provides a feasible and promising strategy for engineering efficient catalysts for heterogeneous thermocatalytic reactions for industrial-scale Cl-CVOC destruction.
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http://dx.doi.org/10.1021/acs.est.2c00270DOI Listing
May 2022

Clinical treatment outcomes and their changes in extremely preterm twins: a multicenter retrospective study in Guangdong Province, China.

Zhongguo Dang Dai Er Ke Za Zhi 2022 Jan;24(1):33-40

Department of Pediatrics, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China.

Objectives: To investigate the clinical treatment outcomes and the changes of the outcomes over time in extremely preterm twins in Guangdong Province, China.

Methods: A retrospective analysis was performed for 269 pairs of extremely preterm twins with a gestational age of <28 weeks who were admitted to the department of neonatology in 26 grade A tertiary hospitals in Guangdong Province from January 2008 to December 2017. According to the admission time, they were divided into two groups: 2008-2012 and 2013-2017. Besides, each pair of twins was divided into the heavier infant and the lighter infant subgroups according to birth weight. The perinatal data of mothers and hospitalization data of neonates were collected. The survival rate of twins and the incidence rate of complications were compared between the 2008-2012 and 2013-2017 groups.

Results: Compared with the 2008-2012 group, the 2013-2017 group (both the heavier infant and lighter infant subgroups) had lower incidence rates of severe asphyxia and smaller head circumference at birth (<0.05). The mortality rates of both of the twins, the heavier infant of the twins, and the lighter infant of the twins were lower in the 2013-2017 group compared with the 2008-2012 group (<0.05). Compared with the 2008-2012 group, the 2013-2017 group (both the heavier infant and lighter infant subgroups) had lower incidence rates of pulmonary hemorrhage, patent ductus arteriosus (PDA), periventricular-intraventricular hemorrhage (P-IVH), and neonatal respiratory distress syndrome (NRDS) and a higher incidence rate of bronchopulmonary dysplasia (<0.05).

Conclusions: There is a significant increase in the survival rate over time in extremely preterm twins with a gestational age of <28 weeks in the 26 grade A tertiary hospitals in Guangdong Province. The incidences of severe asphyxia, pulmonary hemorrhage, PDA, P-IVH, and NRDS decrease in both the heavier and lighter infants of the twins, but the incidence of bronchopulmonary dysplasia increases. With the improvement of diagnosis and treatment, the multidisciplinary collaboration between different fields of fetal medicine including prenatal diagnosis, obstetrics, and neonatology is needed in the future to jointly develop management strategies for twin pregnancy.
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http://dx.doi.org/10.7499/j.issn.1008-8830.2108043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802382PMC
January 2022

Swelling and glyphosate-controlled release behavior of multi-responsive alginate-g-P(NIPAm-co-NDEAm)-based hydrogel.

Carbohydr Polym 2022 Apr 11;282:119113. Epub 2022 Jan 11.

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, PR China.

Intelligent controlled release systems (ICRS) displayed great achievement in agriculture by enhancing the utilization efficiency of agrochemicals. In this work, an intelligent graft copolymer (Alg-g-P(NIPAm-co-NDEAm)) with alginate (Alg) backbone and thermo-responsive poly(N-isopropyl acrylamide-co-N,N-diethylacrylamide) (P(NIPAm-co-NDEAm)) side chain was constructed as the matrix of ICRS through redox copolymerization, and its thermo-induced responsive property was studied. Then, the copolymer was mixed with a promising photothermal material semi-coke (SC) to form hydrogel beads (Ca-Alg-g-P(NIPAm-co-NDEAm)/SC) by ion crosslinking. The water absorbency of beads under different stimuli (pH, temperature, and light) presented outstanding responsive performance and the swelling mechanism was analyzed through coupling theory. Furthermore, the release of glyphosate (Gly) from Ca-Alg-g-P(NIPAm-co-NDEAm)/SC under environmental stimuli displayed regulatable behaviors. This multi-responsive hydrogel bead shows bright prospect in the sustainable advancement of crop production.
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http://dx.doi.org/10.1016/j.carbpol.2022.119113DOI Listing
April 2022

Structural Entropy of the Stochastic Block Models.

Entropy (Basel) 2022 Jan 3;24(1). Epub 2022 Jan 3.

Theory Lab, Central Research Institute, 2012 Labs, Huawei Tech. Co., Ltd., Hong Kong SAR, China.

With the rapid expansion of graphs and networks and the growing magnitude of data from all areas of science, effective treatment and compression schemes of context-dependent data is extremely desirable. A particularly interesting direction is to compress the data while keeping the "structural information" only and ignoring the concrete labelings. Under this direction, Choi and Szpankowski introduced the structures (unlabeled graphs) which allowed them to compute the structural entropy of the Erdős-Rényi random graph model. Moreover, they also provided an asymptotically optimal compression algorithm that (asymptotically) achieves this entropy limit and runs in expectation in linear time. In this paper, we consider the stochastic block models with an arbitrary number of parts. Indeed, we define a partitioned structural entropy for stochastic block models, which generalizes the structural entropy for unlabeled graphs and encodes the partition information as well. We then compute the partitioned structural entropy of the stochastic block models, and provide a compression scheme that asymptotically achieves this entropy limit.
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http://dx.doi.org/10.3390/e24010081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775199PMC
January 2022

The root microbiome: Community assembly and its contributions to plant fitness.

J Integr Plant Biol 2022 Feb;64(2):230-243

State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.

The root microbiome refers to the community of microbes living in association with a plant's roots, and includes mutualists, pathogens, and commensals. Here we focus on recent advances in the study of root commensal community which is the major research object of microbiome-related researches. With the rapid development of new technologies, plant-commensal interactions can be explored with unprecedented breadth and depth. Both the soil environment and the host plant drive commensal community assembly. The bulk soil is the seed bank of potential commensals, and plants use root exudates and immune responses to build healthy microbial communities from the available microbes. The plant microbiome extends the functional system of plants by participating in a variety of processes, including nutrient absorption, growth promotion, and resistance to biotic and abiotic stresses. Plants and their microbiomes have evolved adaptation strategies over time. However, there is still a huge gap in our understanding of the regulatory mechanisms of plant-commensal interactions. In this review, we summarize recent research on the assembly of root microbial communities and the effects of these communities on plant growth and development, and look at the prospects for promoting sustainable agricultural development through the study of the root microbiome.
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http://dx.doi.org/10.1111/jipb.13226DOI Listing
February 2022

Towards a High-Flux Separation Layer from Hexagonal Lyotropic Liquid Crystals for Thin-Film Composite Membranes.

Membranes (Basel) 2021 Oct 29;11(11). Epub 2021 Oct 29.

Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.

Hexagonal lyotropic liquid crystals (HLLC) with uniform pore size in the range of 1~5 nm are highly sought after as promising active separation layers of thin-film composite (TFC) membranes, which have been confirmed to be efficient for water purification. The potential interaction between an amphiphile-based HLLC layer and the substrate surface, however, has not been fully explored. In this research, hydrophilic and hydrophobic microporous polyvinylidene fluoride (PVDF) substrates were chosen, respectively, to prepare TFC membranes with the active layers templated from HLLC, consisting of dodecyl trimethylammonium bromide, water, and a mixture of poly (ethylene glycol) diacrylate and 2-hydroxyethyl methacrylate. The pore size of the active layer was found to decrease by about 1.6 Å compared to that of the free-standing HLLC after polymerization, but no significant difference was observable by using either hydrophilic or hydrophobic substrates (26.9 Å vs. 27.1 Å). The water flux of the TFC membrane with the hydrophobic substrate, however, was higher than that with the hydrophilic one. A further investigation confirmed that the increase in water flux originated from a much higher porosity was due to the synergistic effect of the hydrophilic HLLC nanoporous material and the hydrophobic substrate.
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http://dx.doi.org/10.3390/membranes11110842DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624768PMC
October 2021

Quercetin modulates the gut microbiota as well as the metabolome in a rat model of osteoarthritis.

Bioengineered 2021 12;12(1):6240-6250

The Division of Pulmonary and Critical Care Medicine, Guangzhou Institute of Respiratory Health, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, National Center for Respiratory Medicine, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.

Although the mechanism of osteoarthritis (OA) has been widely studied and the use of quercetin for OA therapy is well documented, the relevant characteristics of the microbiome and metabolism remain unclear. This study reports changes in the gut microbiota and metabolism during quercetin therapy for OA in a rat model and provides an integrative analysis of the biomechanism. In this study, the rats were categorized into 3 different groups: the OA model, quercetin treatment, and control groups. The OA rats was conducted using a monoiodoacetate (MIA) injection protocol. The rats in the quercetin group received daily intragastric administration of quercetin from day 1 to day 28. Stool samples were collected, and DNA was extracted. We used an integrated approach that combined the sequencing of whole 16S rRNA, short-chain fatty acid (SCFA) measurements and metabolomics analysis by mass spectrometry (MS) to characterize the functional impact of quercetin on the gut microbiota and metabolism in a rat model of OA. The use of quercetin partially abrogated intestinal flora disorder and reversed fecal metabolite abnormalities. Compared with the control rats, the OA rats showed differences at both the class level (, and ) and the genus level ( and ). Acetic acid, propionic acid and 24 metabolites were significantly altered among the three groups. However, the changes were significantly abrogated in quercetin-treated OA rats. Consequently, this study provided important evidence regarding perturbations of the gut microbiome and the function of these changes in a potential new mechanism of quercetin treatment.
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http://dx.doi.org/10.1080/21655979.2021.1969194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806632PMC
December 2021

The rice histone methylation regulates hub species of the root microbiota.

J Genet Genomics 2021 09 2;48(9):836-843. Epub 2021 Jul 2.

State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China; CAS-JIC Centre of Excellence for Plant and Microbial Science, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:

Plants have a close relationship with their root microbiota, which comprises a complex microbial network. Histone methylation is an important epigenetic modification influencing multiple plant traits; however, little is known about the role of plant histone methylation in the assembly and network structure of the root microbiota. In this study, we established that the rice (Oryza sativa) histone methylation regulates the structure and composition of the root microbiota, especially the hub species in the microbial network. DJ-jmj703 (defective in histone H3K4 demethylation) and ZH11-sdg714 (defective in H3K9 methylation) showed significant different root microbiota compared with the corresponding wild types at the phylum and family levels, with a consistent increase in the abundance of Betaproteobacteria and a decrease in the Firmicutes. In the root microbial network, 35 of 44 hub species in the top 10 modules in the tested field were regulated by at least one histone methylation-related gene. These observations establish that the rice histone methylation plays a pivotal role in regulating the assembly of the root microbiota, providing insights into the links between plant epigenetic regulation and root microbiota.
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http://dx.doi.org/10.1016/j.jgg.2021.06.005DOI Listing
September 2021

Methylated mud snail protein as a bio-flocculant for high turbidity wastewater treatment.

Water Sci Technol 2021 Aug;84(3):737-751

CAS Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Xining 810001, China.

The authors reported a potential candidate methylated mud snail protein (MeMsp) as an effective and eco-friendly flocculant to treat the high turbidity wastewater. MeMsp was obtained by extraction of mud snail protein (Msp) through isoelectric precipitation (PSC-IP) and then methylated via the esterification with side-chain carboxyl. Structural characterization of FT-IR, zeta potential and elemental analysis were carried out and further confirmed the successful of the methylation. Flocculation experiments with kaolin suspension simulated wastewater indicated that MeMsp-24 displayed more excellent flocculation efficiency at a low dosage. At the optimum dosage 27 mg/L, the maximum clarification efficiency of MeMsp-24 was 97.46% under pH 7.0. Furthermore, MeMsp-24 exhibited a wide flocculation window in the pH range 1.0-9.0, and faster sedimentation velocity and larger flocs size. In addition, MeMsp-24 exhibited 92.12% clarification efficiency in treating railway tunnel construction effluent. The flocculation kinetic and mechanism analysis revealed that the most effective particle collision occurred at the optimal dosage, with charge neutralization and adhesion playing irreplaceable roles in different environments, respectively. Therefore, through extraction and methylation modification, MeMsp could be a promising eco-friendly flocculant for high turbidity wastewater treatment.
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http://dx.doi.org/10.2166/wst.2021.262DOI Listing
August 2021

Stimuli-responsive Ca-alginate-based photothermal system with enhanced foliar adhesion for controlled pesticide release.

Colloids Surf B Biointerfaces 2021 Nov 27;207:112004. Epub 2021 Jul 27.

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, PR China.

For minimizing volatilization and leaching of pesticides, and enhancing their residence time on crop surfaces, we synthesized and characterized a novel intelligent pesticide delivery system. Therein, imidacloprid (IMI) was adsorbed by polydopamine modified kaolin (PK) with high adsorption property through hydrogen bonds. Ca-alginate was used as a structural matrix of the system and a protective shell to hinder the pesticide burst release from PK, and endowed the system with unique pH-sensitive property for IMI release. The amino silicone oil (ASO) coating could bind with the waxy layer of crop leaves by the theory of "similarity-intermiscibility", which increased the adhesion of composite on crop leaves. Moreover, by the excellent light-sensitive property of detonation nanodiamond (DND) and temperature-responsive performance of poly(N-isopropylacrylamide) (PNIPAm), the release of IMI from the functional system could be adjusted by sunlight. Besides, the composite displayed high control efficacy. This novel composite can promote the targeting ability and utilization efficiency of pesticides, thus having a huge potential application prospect in agriculture.
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http://dx.doi.org/10.1016/j.colsurfb.2021.112004DOI Listing
November 2021

High Neutrophil to Lymphocyte Ratio and Its Gene Signatures Correlate With Diastolic Dysfunction in Heart Failure With Preserved Ejection Fraction.

Front Cardiovasc Med 2021 24;8:614757. Epub 2021 Jun 24.

Department of Cardiology, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, China.

To evaluate the interrelation between neutrophil to lymphocyte ratio (NLR) coupled with gene signatures, inflammation, and diastolic dysfunction in patients with heart failure (HF) with preserved ejection fraction (HFpEF). The clinical profile of 172 patients with HFpEF (EF ≥ 50%) and 173 non-HF control individuals was analyzed retrospectively. The association between NLR and HFpEF and the predictive performance of NLR for HFpEF were assessed by the binary logistic regression analysis and the receiver operating characteristic curve (ROC). Multivariate linear regression models further examined the associations between NLR and high-sensitivity C-reactive protein (hs-CRP), N-terminal prohormone of brain natriuretic peptide (NT-proBNP), and average septal-lateral E/e', respectively. The freshly isolated neutrophils from 30 HFpEF patients and 42 non-HF controls were subjected to transcriptomic profiling. The biomarkers related to neutrophil activation and inflammation were detected in serum samples. The HFpEF patients in Southeast China were lean and had comorbidity burden and worse cardiac structure/function. Compared with non-HF control individuals, HFpEF patients had a rise in NLR. NLR displayed an independent association with HFpEF [adjusted odds ratio, 2.351; 95% CI, 1.464-3.776; < 0.001] and it predicted HFpEF with the area under the ROC 0.796 (95% CI, 0.748-0.845, < 0.001). The positive associations between NLR and hs-CRP, NT-proBNP, and mitral E/e' were found in HFpEF patients. Moreover, patients had significantly elevated serum levels of neutrophil elastase and inflammatory biomarkers, both of which correlated with the mitral E/e' ratio. Finally, multiple molecules that drive neutrophil degranulation and inflammation, such as // and , were transcriptionally up-regulated in neutrophils of HFpEF patients. The high NLR coupled with transcriptional activation of neutrophils correlates with systemic inflammation and functional impairment in HFpEF patients, which may suggest a causative role of neutrophils in the pathogenesis of the disease.
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http://dx.doi.org/10.3389/fcvm.2021.614757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263935PMC
June 2021

Health-related quality of life after first-ever acute ischemic stroke: associations with cardiovascular health metrics.

Qual Life Res 2021 Oct 1;30(10):2907-2917. Epub 2021 May 1.

Department of Neurology, Shandong Provincial Hospital Affiliated To Shandong University, Jinan, Shandong, China.

Purpose: To investigate the associations between cardiovascular health (CVH) metrics and health-related quality of life (HRQL) among patients with ischemic stroke in China, and further explore the role of physical and cognitive function in their associations.

Methods: This hospital-based study included 1714 patients with first-ever acute ischemic stroke (age ≥ 40 years; 36.7% women) who were admitted to two university hospitals in Shandong, China. We collected information on seven CVH metrics (smoking, body mass index, diet, physical activity, blood pressure, total cholesterol, and fasting blood glucose) through interviews, clinical examinations, and laboratory tests. EQ-5D-3L was used to assess HRQL. Cognitive and physical functioning was assessed by the Montreal Cognitive Assessment test and Barthel index, respectively. Data were analyzed using the general linear regression models.

Results: The average score (SD) was 0.746 (0.23) for HRQL index and 72.7 (15.8) for self-rated health. Optimal levels of four individual CVH metric components (diet, physical activity, blood pressure, and blood glucose) and a higher composite CVH score were significantly associated with a greater HRQL index and better self-rated health (p < 0.05 for all). Physical dependence and cognitive impairment were associated with a lower HRQL index and poorer self-rated health status (p < 0.001). Furthermore, the relationships between CVH metrics and HRQL index varied by functional status, such that their associations were statistically significant only among people who had physical dependence or cognitive impairment.

Conclusion: Achieving a better cardiovascular health profile is associated with better quality of life among ischemic stroke survivors, primarily in those with physical or cognitive impairment.
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http://dx.doi.org/10.1007/s11136-021-02853-xDOI Listing
October 2021

Investigation of normal knees kinematics in walking and running at different speeds using a portable motion analysis system.

Sports Biomech 2021 Feb 14:1-14. Epub 2021 Feb 14.

Department of Orthopaedic Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Walking and running at different speeds are common in daily life. This study investigated 6 degrees of freedom (DOF) kinematics of normal knees of Chinese during walking and running. Forty healthy were investigated in 4 conditions: comfortable walking, normal walking, slow running and ordinary running. The range of motion (ROM) and peak values in 6 DOF kinematics were . As the speed increased, a general increase in flexion, lateral and proximal translations occurred. Significant increases of ROM in flexion/extension, axial rotation and medial/lateral translations were observed. The ROM of adduction/abduction, anterior/posterior and proximal/distal translations were greatest during normal walking. The maximum and minimum flexion/extension, maximum internal rotation and tibial lateral translations increased with the increase of speed. The maximum and minimum tibial proximal translations in running were found being greater than walking. A phenomenon between walking and running was observed: both tibial proximal/distal and medial/lateral translations increased when changed from walking to running. Non-linear transition exists in 6 DOF kinematics during walking to running. Discoveries in this study may have potential clinical values to serve as references of normal walking and running in the management of knee injury and knee rehabilitation.
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http://dx.doi.org/10.1080/14763141.2020.1864015DOI Listing
February 2021

Metformin: A Novel Weapon Against Inflammation.

Authors:
Bo Bai Haibo Chen

Front Pharmacol 2021 29;12:622262. Epub 2021 Jan 29.

Department of Cardiology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.

It has become widely accepted that inflammation is a driving force behind a variety of chronic diseases, such as cardiovascular disease, diabetes, kidney disease, cancer, neurodegenerative disorders, etc. However, the existing nonsteroidal anti-inflammatory drugs show a limited utility in clinical patients. Therefore, the novel agents with different inflammation-inhibitory mechanisms are worth pursuing. Metformin, a synthetic derivative of guanidine, has a history of more than 50 years of clinical experience in treating patients with type 2 diabetes. Intense research efforts have been dedicated to proving metformin's inflammation-inhibitory effects in cells, animal models, patient records, and randomized clinical trials. The emerging evidence also indicates its therapeutic potential in clinical domains other than type 2 diabetes. Herein, this article appraises current pre-clinical and clinical findings, emphasizing metformin's anti-inflammatory properties under individual pathophysiological scenarios. In summary, the anti-inflammatory effects of metformin are evident in pre-clinical models. By comparison, there are still clinical perplexities to be addressed in repurposing metformin to inflammation-driven chronic diseases. Future randomized controlled trials, incorporating better stratification/targeting, would establish metformin's utility in this clinical setting.
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http://dx.doi.org/10.3389/fphar.2021.622262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880161PMC
January 2021

Apelin-36 Protects HT22 Cells Against Oxygen-Glucose Deprivation/Reperfusion-Induced Oxidative Stress and Mitochondrial Dysfunction by Promoting SIRT1-Mediated PINK1/Parkin-Dependent Mitophagy.

Neurotox Res 2021 Jun 13;39(3):740-753. Epub 2021 Feb 13.

Neurobiology Institute, Jining Medical University, Jining, 272067, China.

Oxidative stress and mitochondrial dysfunction are involved in cerebral ischemia/reperfusion injury-induced neuronal apoptosis. Mitophagy is the main method to eliminate dysfunctional mitochondria. Apelin-36, a type of neuropeptide, has been reported to exert protective effects in cerebral I/R (I/R) injury, but its precise mechanisms remain to be elucidated. To study the effects of Apelin-36 on oxidative stress and mitochondrial dysfunction in cerebral I/R injury, the oxygen-glucose deprivation/reperfusion (OGD/R) model with 6 h of ischemia and 6 h of reperfusion was established in HT22 cells. Results demonstrated that Apelin-36 protected against OGD/R injury by improving cell viability, decreasing the apoptotic cells ratio and increasing the ratio of Bcl-2/Bax. In addition, Apelin-36 treatment inhibited oxidative stress by downregulating the level of reactive oxygen species (ROS) and malondialdehyde (MDA) as well as the expression of inducible nitric oxide synthase (iNOS). And Apelin-36 also activated the level of superoxide dismutase (SOD) and glutathione (GSH). Mitochondrial apoptosis was also alleviated with Apelin-36 treatment detected by the mitochondrial membrane potential (MMP) and the expression of Cytochrome c (Cyt c), Cleaved caspase-9, and Cleaved caspase-3. Furthermore, the SIRT1-mediated PINK1/Parkin-dependent mitophagy was activated by Apelin-36 treatment with the downregulation of p62 and upregulation of LC3B-II and Beclin1. Both EX527 and Cyclosporine A (CsA), which are inhibitors of SIRT1 and mitophagy, markedly alleviated the inhibition of oxidative stress and mitochondrial dysfunction caused by Apelin-36. These findings suggest that SIRT1-mediated PINK1/Parkin-dependent mitophagy is involved in the neuroprotective effects of Apelin-36 on OGD/R-induced oxidative stress and mitochondrial dysfunction.
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http://dx.doi.org/10.1007/s12640-021-00338-wDOI Listing
June 2021

Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6ρ0 cells.

Mol Med Rep 2021 04 12;23(4). Epub 2021 Feb 12.

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, P.R. China.

Malignant tumors of the central nervous system (CNS) are among the types of cancer with the poorest prognosis and glioma is the commonest primary CNS tumor. A mitochondrial DNA (mtDNA)‑depleted cell line C6ρ0 was generated from C6 glioma cells after long‑term exposure to ethidium bromide and 2',3'‑dideoxycytidine in order to determine the effect of mtDNA damage on cell proliferation and pathological changes in glioma cells. Single cell clones were isolated and identified after 42 days of incubation. Repopulated cybrids were formed when the clonal C6ρ0 cells were fused with rat platelets and no difference was observed in their growth in a selective medium without uridine and pyruvate compared with the growth of the parent C6 cells. Disruption of mtDNA resulted in changes in mitochondrial morphology, decreased cell proliferation, reduced intracellular reactive oxygen species and intracellular ATP, along with decreased mtDNA and mitochondrial membrane potential in C6ρ0 cells compared with the C6 cells. Taken together, C6ρ0 cells without mtDNA were established for the first time and their characteristics were compared with parent cells. This C6ρ0 cell line could be used to explore the contribution of mitochondrial dysfunction and mtDNA mutations in the pathogenesis of glioma.
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http://dx.doi.org/10.3892/mmr.2021.11904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893707PMC
April 2021

The novel GLP-1/GIP dual agonist DA3-CH is more effective than liraglutide in reducing endoplasmic reticulum stress in diabetic rats with cerebral ischemia-reperfusion injury.

Nutr Metab Cardiovasc Dis 2021 01 12;31(1):333-343. Epub 2020 Sep 12.

Second Hospital, Neurology Department, Shanxi Medical University, Taiyuan 030001, Shanxi province, PR China; Research and Experimental Center, Henan University of Chinese Medicine, Zhengzhou 450046, Henan province, PR China.

Background And Aims: Diabetes is one of the most important risk factors and comorbidities of ischemic stroke. Endoplasmic reticulum stress (ERS) is considered to be the major injury mechanism of ischemic stroke with diabetes. Studies have found that incretin can inhibit ERS in ischemia-reperfusion injury of the liver and heart. We aimed to explore the effects of GLP-1/GIP double agonist DA3-CH and GLP-1 single agonist liraglutide on ERS and apoptosis in diabetic rats with cerebral ischemia-reperfusion injury.

Methods And Results: 72 Sprague-Dawley (SD) male rats were randomly divided into 4 groups: ① blank group (Sham group, n = 18); model group (Saline group, n = 18); DA3 treatment group (DA3 group, n = 18); liraglutide treatment group (Lir group, n = 18). The Sham group was not given any treatment and was only raised in the same environment as the other groups. The remaining 3 groups used STZ-induced diabetes models. After the successful membrane formation of diabetes, DA3-CH and liraglutide (10 mmol/kg, once-daily for 14 days) were injected intraperitoneally. Thereafter, rats were subjected to middle cerebral artery occlusion followed by 24-h reperfusion. Animals were evaluated for neurologic deficit score, infarct volume, and biomarker analyses of the brain after ischemia. The DA3-CH-treated and liraglutide-treated groups showed significantly reduced scores of neurological dysfunction and cerebral infarction size, and reduced the expression of ERS markers GRP78, CHOP and Caspase-12, and the expression of apoptosis marker bax. Anti-apoptotic markers bcl-2 and neuronal numbers increased significantly.

Conclusions: DA3-CH and liraglutide have obvious neuroprotective effects in a rat model of cerebral ischemia-reperfusion injury with diabetes, which can reduce the infarct size and the neurological deficit score. Their exert neuroprotective effects in a rat model of cerebral ischemia-reperfusion injury with diabetes by inhibiting endoplasmic reticulum stress and thereby reducing apoptosis. DA3 is better than liraglutide.
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http://dx.doi.org/10.1016/j.numecd.2020.09.002DOI Listing
January 2021

The Role of Mitophagy in Ischemic Stroke.

Front Neurol 2020 23;11:608610. Epub 2020 Dec 23.

Neurobiology Institute, Jining Medical University, Jining, China.

Mitochondria are important places for eukaryotes to carry out energy metabolism and participate in the processes of cell differentiation, cell information transmission, and cell apoptosis. Autophagy is a programmed intracellular degradation process. Mitophagy, as a selective autophagy, is an evolutionarily conserved cellular process to eliminate dysfunctional or redundant mitochondria, thereby fine-tuning the number of mitochondria and maintaining energy metabolism. Many stimuli could activate mitophagy to regulate related physiological processes, which could ultimately reduce or aggravate the damage caused by stimulation. Stroke is a common disease that seriously affects the health and lives of people around the world, and ischemic stroke, which is caused by cerebral vascular stenosis or obstruction, accounts for the vast majority of stroke. Abnormal mitophagy is closely related to the occurrence, development and pathological mechanism of ischemic stroke. However, the exact mechanism of mitophagy involved in ischemic stroke has not been fully elucidated. In this review, we discuss the process and signal pathways of mitophagy, the potential role of mitophagy in ischemic stroke and the possible signal transduction pathways. It will help deepen the understanding of mitophagy and provide new ideas for the treatment of ischemic stroke.
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http://dx.doi.org/10.3389/fneur.2020.608610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793663PMC
December 2020

Electric vehicle-attributed environmental injustice: Pollutant transfer into regions with poor traffic accessibility.

Sci Total Environ 2021 Feb 21;756:143853. Epub 2020 Nov 21.

School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China.

Electric vehicles (EVs) are promoted in recent years as an effective way in alleviating the air pollution caused by tailpipe emissions. However, the pollutants derived from EVs are unheeded. EVs rely on electricity to provide power, and thus their related pollution is transferred to the power plants, which gives rise to the environmental and health pressure to the adjacent regions. In this paper, the transfer of EV-attributed PM, SO, and NO inhalations in China are studied. Then by comparing the inhalations versus traffic accessibility among the impacted municipalities, this study sheds light on the environmental injustice lying in the mismatch between pollutant inhalations and traffic accessibility. The results reveal that compared with Shanghai and Shenzhen, the promotion of EVs in Beijing triggers higher pollutant inhalations to its surrounding municipalities. North China Power Grid undertakes 715.62 g PM inhalation in total, which is 2.51 and 3.20 times higher than the East China Power Grid and the China Southern Power Grid, respectively. The number of municipalities with lower traffic accessibility while higher pollutant inhalation is 8,8, and 17 in North China Power Grid, East China Power Grid, and China Southern Power Grid respectively, indicating conspicuous environmental injustice resulted from the promotion of EVs.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143853DOI Listing
February 2021

Apelin-13 inhibits apoptosis and excessive autophagy in cerebral ischemia/reperfusion injury.

Neural Regen Res 2021 Jun;16(6):1044-1051

Neurobiology Institute, Jining Medical University, Jining, Shandong Province, China.

Apelin-13 is a novel endogenous ligand for an angiotensin-like orphan G-protein coupled receptor, and it may be neuroprotective against cerebral ischemia injury. However, the precise mechanisms of the effects of apelin-13 remain to be elucidated. To investigate the effects of apelin-13 on apoptosis and autophagy in models of cerebral ischemia/reperfusion injury, a rat model was established by middle cerebral artery occlusion. Apelin-13 (50 μg/kg) was injected into the right ventricle as a treatment. In addition, an SH-SY5Y cell model was established by oxygen-glucose deprivation/reperfusion, with cells first cultured in sugar-free medium with 95% N and 5% CO for 4 hours and then cultured in a normal environment with sugar-containing medium for 5 hours. This SH-SY5Y cell model was treated with 10 M apelin-13 for 5 hours. Results showed that apelin-13 protected against cerebral ischemia/reperfusion injury. Apelin-13 treatment alleviated neuronal apoptosis by increasing the ratio of Bcl-2/Bax and significantly decreasing cleaved caspase-3 expression. In addition, apelin-13 significantly inhibited excessive autophagy by regulating the expression of LC3B, p62, and Beclin1. Furthermore, the expression of Bcl-2 and the phosphatidylinositol-3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway was markedly increased. Both LY294002 (20 μM) and rapamycin (500 nM), which are inhibitors of the PI3K/Akt/mTOR pathway, significantly attenuated the inhibition of autophagy and apoptosis caused by apelin-13. In conclusion, the findings of the present study suggest that Bcl-2 upregulation and mTOR signaling pathway activation lead to the inhibition of apoptosis and excessive autophagy. These effects are involved in apelin-13-induced neuroprotection against cerebral ischemia/reperfusion injury, both in vivo and in vitro. The study was approved by the Animal Ethical and Welfare Committee of Jining Medical University, China (approval No. 2018-JS-001) in February 2018.
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http://dx.doi.org/10.4103/1673-5374.300725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224111PMC
June 2021

Intralesional curettage versus prosthetic replacement for bone tumors - a finite element analysis case of limb salvage simulation in biomechanics.

Comput Methods Programs Biomed 2021 Jan 3;198:105775. Epub 2020 Oct 3.

Orthopedics Dept. of 1st Affiliated Hospital, GuangZhou Medical University, China. Electronic address:

Background And Objectives: To compare mechanical properties of femoral tumor treatments so that better operative strategy for limb tumors surgery is optimized.

Methods: Fourteen femoral CT images were randomly selected to rebuild 3D models by MIMICS. They were then executed by reverse engineering softwares for simulative modes. Mode #1: Intralesional curettage with cement filled plus fixator; Mode #2: Distal femur resection with tumorous prosthesis replaced. Finally, the mechanical aspects such as stress and displacement were compared by finite element analysis.

Results: Analyzed by AnSys, the observation indexes were measured as follows: for displacement of femurs, d=1.4762 (< a=3.9042 < c=3.9845 < b=4.1159) in mm is the most staple of all models; for displacement of implants (fixators or prostheses), it's similar to the behavior of femurs and with no significant difference; for stresses of femurs, no significant difference was found among all models; the stresses of implants (fixations and prostheses) were observed as d=39.6334 (< a=58.6206 < c=61.8150 < b=62.6626) in MPa correspondently, which is the least; for stresses of the general system, the average of peak values for integrated devices of all models are: d=40.8072 (< a=58.6206 < c=61.7831< b=62.6626) in MPa, which is also the least. As a final result, both maximum values for displacement and stress of mode 2 are lower than those of mode 1.

Conclusions: Our finite element analysis of limb salvage simulation in biomechanics proved that, to treat distal femoral bone tumors, prosthetic replacement is more efficient than intralesional curettage.
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http://dx.doi.org/10.1016/j.cmpb.2020.105775DOI Listing
January 2021

Interaction of CEND1 gene and life events in susceptibility to depressive symptoms in Chinese Han college students.

J Affect Disord 2021 01 24;278:570-575. Epub 2020 Sep 24.

Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China; Shanghai Key Laboratory of Psychotic Disorders, and Brain Science and Technology Research Center, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China. Electronic address:

Background: . The development of depressive symptoms (DSs) is a complex process caused by both genetic and environmental factors. CEND1 gene coordinates cell division, differentiation and maturation of neural precursor cells, which affects brain structure and function. Our study investigated whether CEND1 was a genetic factor for DSs, particularly under negative life events.

Methods: . 272 freshmen with DSs and 467 healthy controls were recruited via the Center for Epidemiologic Studies Depression Scale (CES-D). The adolescent Self-rating Life Event Checklist (ASLEC) was adopted to assess stressful life events during the past 12 months. Two SNPs (rs7946354, rs6597982) within the CEND1 gene were genotyped using Agena MassARRAY iPLEX technology. We combined generalized multifactor dimensionality reduction (GMDR) with RStudio programming to assess the direct association and gene-environment interaction (G × E).

Results: . Rs7946354 was associated with DSs in an overdominant model (GT vs. GG+TT). In addition, both rs7946354 and rs6597982 had considerable impacts on negative life events. GMDR showed a statistical G × E that the AG genotype of rs6597982 and GT genotype of rs7946354 contribute to the maximum risk of DSs under high negative life events.

Limitations: . Only two single nucleotide polymorphisms (SNPs) were examined. Verification studies with bigger sample size and more varied demographic background information could be adopted to further support the generalization of these findings.

Conclusions: .CEND1 can potentially cause high sensitivity to life events and affect DSs especially in the presence of negative life events, which contribute to the field of depression prevention and treatment.
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http://dx.doi.org/10.1016/j.jad.2020.09.082DOI Listing
January 2021

Gene-modified BMSCs encapsulated with carboxymethyl cellulose facilitate osteogenesis in vitro and in vivo.

J Biomater Appl 2021 02 10;35(7):814-822. Epub 2020 Aug 10.

Department of Orthopedics, Guangdong Key Laboratory of Orthopaedic Technology and Implant Materials, The First Affiliated Hospital of 26468Guangzhou Medical University, Guangzhou, China.

Critical size bone defects are one of the most serious complications in orthopedics due to the lack of effective osteogenesis treatment. We fabricated carboxymethyl cellulose with phenol moieties (CMC-ph) microcapsules loaded with gene-modified rat bone mesenchymal stem cells (rBMSCs) that secrete hBMP2 following doxycycline (DOX) induction. The results showed that the morphology of microcapsules was spherical, and their diameters have equally distributed in the range of 100-150 μm; the viability of rBMSCs was unchanged over time. Through real-time PCR and Western blot analyses, the rBMSCs in microcapsules were found to secrete hBMP2 and to have upregulated mRNA and protein expression of osteogenesis-related genes in vitro and in vivo. Furthermore, the in vivo results suggested that the group with the middle concentration of cells expressed the highest amount of osteogenic protein over time. In this study, we showed that gene-modified rBMSCs in CMC-ph microcapsules had good morphology and viability. The BMP2-BMSCs/CMC-Ph microcapsule system could upregulate osteogenic mRNA and protein in vitro and in vivo. Further analysis demonstrated that the medium concentration of cells had a suitable density for transplantation in nude mice. Therefore, BMP2-BMSCs/CMC-Ph microcapsule constructs have potential for bone regeneration in vivo.
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http://dx.doi.org/10.1177/0885328220948030DOI Listing
February 2021

The pregnancy-associated spontaneous coronary artery dissection in a young woman with a novel missense mutation in NOTCH1: a case report.

BMC Med Genet 2020 06 1;21(1):119. Epub 2020 Jun 1.

Department of Cardiology, Shenzhen Second People's Hospital, the First Affiliated Hospital of Shenzhen University Health Science Center, No. 3002, Sungang West Road, Futian District, Shenzhen, 518035, China.

Background: Spontaneous coronary artery dissection (SCAD) is frequently reported as a disorder that primarily affects women without risk factors for cardiovascular disease. Although it has been recognized as one of the genetically mediated vascular disorders, the genetic pathogenesis of SCAD remains obscure to date.

Case Presentation: In this report, we presented a rare case of pregnancy-associated SCAD in a young woman that occurred in multiple coronary arteries within a short period. The initial conservative management and then intravascular ultrasound-guided primary percutaneous coronary intervention (PCI) were adopted to achieve optimal results of revascularization in affected coronary arteries and avoid potential risks for PCI-associated complications. We further performed the whole-exome sequencing and Sanger sequencing and, for the first time, reported a novel heterozygous missense variant, c.4574 C > T (p.Arg1438Cys), in the NOTCH1 gene. This variant has never been documented in the medical literature and was predicted as being potentially damaging or disease-causing variant.

Conclusions: We described a rare case of recurrent SCAD in a young woman after baby delivery. The initial conservative management and PCI with multiple stent implantations were successfully implemented to achieve optimal results of revascularization in coronary arteries. We, for the first time, identified a novel missense variant in the NOTCH1 gene, which appears to be a potential predisposing factor for artery fragility.
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http://dx.doi.org/10.1186/s12881-020-01058-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268224PMC
June 2020

Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.

Int J Biol Macromol 2020 Oct 29;160:518-530. Epub 2020 May 29.

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China; Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Xining 810001, China.

The multi-responsive controlled-release system could enhance crop yield while improving utilization efficiency of agrochemicals, and minimize environmental pollution caused by agrochemicals overuse. This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/[email protected]) microsphere to control the agrochemicals release. Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis. Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated. The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation. Consequently, the Ca-alginate/[email protected] microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.234DOI Listing
October 2020
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