Publications by authors named "Bin Wang"

6,168 Publications

  • Page 1 of 1

Nickel-induced charge redistribution in Ni-Fe/[email protected] carbon nanocage as a robust Mott-Schottky bi-functional oxygen catalyst for rechargeable Zn-air battery.

J Colloid Interface Sci 2022 Jun 17;625:521-531. Epub 2022 Jun 17.

Institute for Ecological Research and Pollution Control of Plateau Lakes, School of Ecology and Environmental Science, Yunnan University, Kunming, Yunnan 650504, PR China; Department of Physics, Umeå University, Umeå S-901 87, Sweden. Electronic address:

Designing earth-abundant and advanced bi-functional oxygen electrodes for efficient oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) are extremely urgent but still ambiguous. Thus, metal-semiconductor nanohybrids were developed with functionally integrating ORR-active Ni species, OER-active Fe/FeC components, and multifunctional N-doped carbon (NDC) support. Expectantly, the resulted NDC nanocage embedded with Ni-Fe alloy and FeC particles, as assembled Mott-Schottky-typed catalyst, delivered a promoted half-wave potential of 0.904 V for ORR and a low overpotential of 315 mV at 10 mA/cm for OER both in alkaline media, outperforming those of commercial Pt/C and RuO counterparts. Most importantly, the optimized Ni-Fe/[email protected] sample also afforded a peak power density of 267.5 mW/cm with a specific capacity of 773.8 mAh/g and excellent durability over 80 h when used as the air electrode in rechargeable Zn-air batteries, superior to the state-of-the-art bi-functional catalysts. Ultraviolet photoelectron spectroscopy revealed that the introduction of Ni into the Fe/[email protected] component could well manipulate the electronic structure of the designed electrocatalyst, leading to an effective built-in electric field established by the Mott-Schottky heterojunction to expedite the continuous interfacial charge-transfer and thus significantly promote the utilization of electrocatalytic active sites. Therefore, this work provides an avenue for the designing and developing robust and durable Mott-Schottky-typed bi-functional catalysts for promising energy conversion.
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http://dx.doi.org/10.1016/j.jcis.2022.06.067DOI Listing
June 2022

Optimization of whole-cell vaccines with CpG/αOX40/cGAMP to strengthen the anti-tumor response of CD4 T cells in melanomas.

J Cancer Res Clin Oncol 2022 Jun 24. Epub 2022 Jun 24.

Department of Oncology, The First Affiliated Hospital of Wenzhou Medical University, 2 Fuxue Road, Wenzhou, 325000, Zhejiang, People's Republic of China.

Methods: In this study, we developed a strategy for the prevention and therapy of melanoma using a whole-cell vaccine combined with a CpG/αOX40/cGAMP triple adjuvant. The CpG/αOX40/cGAMP triple adjuvant was used to co-culture melanoma cells in vitro to induce immunogenic death of tumor cells. The mixture of inactivated tumor cells and the triple drug was an optimized tumor whole-cell vaccine, which was injected subcutaneously into mice for tumor prevention and therapy. Furthermore, we analyzed the changes of immune cells in spleen and tumor by flow cytometry and immunohistochemistry, and detected the changes of cytokines after vaccine application by cytometric bead array to explore the specific mechanism of vaccine.

Results: In vaccine prevention and therapy experiments, it was observed that the tumor growth was significantly inhibited in the whole-cell vaccine group, and the survival time of mice was significantly prolonged. Flow cytometry results showed that the proportion of CD4+ T cells and CD8+ T cells in tumor of mice in vaccine group was higher than that in control group, especially the CD4+ T cells.

Conclusion: The optimized vaccine has the unique ability to amplify tumor-specific CD4+ T cells, which improves antitumor sensitivity, and has a significant effect on the prevention and therapy of melanoma mice.
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http://dx.doi.org/10.1007/s00432-022-04117-8DOI Listing
June 2022

Canagliflozin Ameliorates Nonalcoholic Fatty Liver Disease by Regulating Lipid Metabolism and Inhibiting Inflammation through Induction of Autophagy.

Yonsei Med J 2022 Jul;63(7):619-631

Department of Comprehensive Internal Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.

Purpose: Nonalcoholic fatty liver disease (NAFLD) is closely associated with metabolic diseases, including obesity and diabetes, and has gradually become the most common cause of chronic liver disease. We investigated the effects of sodium glucose cotransporter 2 (SGLT2) inhibitor canagliflozin on NAFLD in high-fat diet (HFD)-induced obese mice and possible underlying mechanisms.

Materials And Methods: Male C57BL/6 mice were fed a normal-diet, HFD, or HFD with canagliflozin for 14 weeks. AML-12 hepatocytes were treated with canagliflozin. Expression of related pathways was assessed.

Results: Canagliflozin administration reduced body weight and fat mass, compared with HFD alone. Canagliflozin improved glucose and lipid metabolic disorders. Compared with HFD-fed mice, liver weight, serum alanine transaminase (ALT) levels, and hepatic lipid accumulation were decreased after canagliflozin administration. Additionally, canagliflozin upregulated lipolysis markers (CPT1a, ACOX1, and ACADM), downregulated lipogenesis markers (SREBP-1c and FASN), and suppressed the production of inflammatory cytokines (TNFα, MCP1, IL-1β, and IL-6), consistent with significantly increased LC3 II/I and Atg7 levels in the liver following canagliflozin treatment. In vitro, canagliflozin increased CPT1a, ACOX1, and ACADM expression, decreased SREBP-1c and FASN protein expression, and reduced TNFα, MCP1, IL-1β, and IL-6 mRNA levels in lipid mixture (LM)-induced hepatocytes in a dose-dependent manner. These changes were reversed by 3-MA, an autophagy inhibitor.

Conclusion: Our findings suggest that canagliflozin ameliorates the pathogenesis of NAFLD by regulating lipid metabolism and inhibiting inflammation, which may be associated with its promotion of autophagy.
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http://dx.doi.org/10.3349/ymj.2022.63.7.619DOI Listing
July 2022

Two Rhabdoviruses, One Novel, Isolated from in China.

Pathogens 2022 May 27;11(6). Epub 2022 May 27.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

The family contain important human and mammalian pathogens that are vectored by different arthropod species. The ground supernatants of mosquitoes were used to inoculate in BHK-21 and C6/36 cells for virus isolation. Then, the viral complete genome sequence was obtained and used for phylogenetic analysis. In this study, we observed a cytopathic effect (CPE) in mosquito cells (C6/36) and rod-like virion after inoculating a pool of samples collected in Shanxi Province, China, in 2019 (SX1916). Meta-transcriptomics sequencing revealed the presence of two distinctive rhabdoviruses with similar abundance levels, namely, Shanxi rhabdovirus (SXARV) and Shanxi Arboretum virus (SXABTV). Despite the fact that the SXARV genome (9590 nt) was much shorter than that of SXABTV (11,480 nt), both belonged to the group within whose genomes encoded five proteins (N, P, M, G, and L) and a small hydrophobin (U1) and the difference in lengths is mainly caused by a substantially shorter N protein encoded by SXARV. On the phylogenetic tree, SXABTV was closely related (90.7% amino acid identity at L protein) with the Arboretum virus isolated from Psorophora albigenu mosquitoes in Peru in 2014, whereas SXARV was distantly related to Rio Chico virus (63.3% amino acid identity), a genetic distance large enough to be defined as a new species within . Collectively, we report a simultaneous isolation of two related rhabdoviruses from that marked the circulation of almendraviruses in Shanxi, China.
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http://dx.doi.org/10.3390/pathogens11060624DOI Listing
May 2022

An Experimental Analysis to Determine the Load-Bearing Capacity of 3D Printed Metals.

Materials (Basel) 2022 Jun 19;15(12). Epub 2022 Jun 19.

Department of Mechanical Engineering Technologies, Academy of Engineering, Peoples' Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya Street, 117198 Moscow, Russia.

Reverse engineering is conducted based on the analysis of an already existing product. The results of such an analysis can be used to improve the functioning of the product or develop new organizational, economic, information technology, and other solutions that increase the efficiency of the entire business system, in particular 3D printed products. Therefore, the main aim of this research is to focus on evaluation of the load-bearing capacity of already existing 3D printed metals in order to see their suitability for the intended application and to obtain their relevant mechanical properties. To this end, 3D printed metallic bars with almost square cross-sections were acquired from an external company in China without any known processing parameters, apart from the assumption that specimens No. 1-3 are printed horizontally, and specimens No. 4-7 are printed vertically. Various experiments were conducted to study microstructural characteristics and mechanical properties of 3D printed metals. It was observed that specimens No. 1-6, were almost similar in hardness, while specimen No. 7 was reduced by about 4.5% due to the uneven surface. The average value of hardness for the specimens was found to be approximately 450 HV, whereas the load-extension graphs assessed prior point towards the conclusion that the specimens' fractured in a brittle status, is due to the lack of plastic deformation. For different specimens of the 3D printed materials, the main defects were identified, namely, lack of fusion and porosity are directly responsible for the cracks and layer delamination, prevalent in SLM printed metals. An extensive presence of cracks and layer delamination prove that the printing of these metallic bars was completed in a quick and inaccurate manner, which led to higher percentages of lack of fusion due to either low laser power, high scan speed, or the wrong scan strategy.
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http://dx.doi.org/10.3390/ma15124333DOI Listing
June 2022

Frequency-Specific Analysis of the Dynamic Reconfiguration of the Brain in Patients with Schizophrenia.

Brain Sci 2022 Jun 1;12(6). Epub 2022 Jun 1.

College of Information and Computer, Taiyuan University of Technology, No. 209, Daxue Street, Yuci District, Jinzhong 030024, China.

The analysis of resting-state fMRI signals usually focuses on the low-frequency range/band (0.01-0.1 Hz), which does not cover all aspects of brain activity. Studies have shown that distinct frequency bands can capture unique fluctuations in brain activity, with high-frequency signals (>0.1 Hz) providing valuable information for the diagnosis of schizophrenia. We hypothesized that it is meaningful to study the dynamic reconfiguration of schizophrenia through different frequencies. Therefore, this study used resting-state functional magnetic resonance (RS-fMRI) data from 42 schizophrenia and 40 normal controls to investigate dynamic network reconfiguration in multiple frequency bands (0.01-0.25 Hz, 0.01-0.027 Hz, 0.027-0.073 Hz, 0.073-0.198 Hz, 0.198-0.25 Hz). Based on the time-varying dynamic network constructed for each frequency band, we compared the dynamic reconfiguration of schizophrenia and normal controls by calculating the recruitment and integration. The experimental results showed that the differences between schizophrenia and normal controls are observed in the full frequency, which is more significant in slow3. In addition, as visual network, attention network, and default mode network differ a lot from each other, they can show a high degree of connectivity, which indicates that the functional network of schizophrenia is affected by the abnormal brain state in these areas. These shreds of evidence provide a new perspective and promote the current understanding of the characteristics of dynamic brain networks in schizophrenia.
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http://dx.doi.org/10.3390/brainsci12060727DOI Listing
June 2022

Associations of polychlorinated biphenyls exposure with plasma glucose and diabetes in general Chinese population: The mediating effect of lipid peroxidation.

Environ Pollut 2022 Jun 20:119660. Epub 2022 Jun 20.

Department of Occupational & Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address:

Polychlorinated biphenyls (PCBs) exposure has been related to the abnormal glucose metabolism and the risk of diabetes. However, the joint effects of various PCBs are uncertain and the potential mechanisms remain unclear. Our objectives were to evaluate the associations of serum PCBs with fasting plasma glucose (FPG) and the risk of diabetes among a general Chinese population, and to estimate the mediating effects of oxidative stress in the above associations. Serum levels of seven indicator-PCBs (PCB-28, 52, 101, 118, 138, 153, and 180) and FPG values were determined among 4498 subjects from the Wuhan-Zhuhai cohort. Oxidative DNA damage biomarker (urinary 8-hydroxy-2'-deoxyguanosine, 8-OHdG) and lipid peroxidation biomarker (urinary 8-isoprostane, 8-iso-PGF) were also measured. Positive relationships of serum PCBs with FPG values as well as the risk of diabetes were observed. With each 1% increment in the natural log-transformed values of wet weight serum PCBs, FPG levels increased a 0.125% for PCB-52, 0.168% for PCB-118, 0.221% for PCB-138, 0.273% for PCB-153, and 0.379% for ΣPCB (the sum of seven PCBs). The adjusted odds ratios of diabetes associated with wet weight PCBs were 1.186 for PCB-52, 1.373 for PCB-118, 1.635 for PCB-153, and 1.456 for ΣPCB. The seven serum PCBs showed positive overall effect on the risk of diabetes. Elevated PCB-28, PCB-52, PCB-118, PCB-138, PCB-153, and ΣPCB were associated with the increased urinary 8-iso-PGF, which was positively related with FPG values. Furthermore, urinary 8-iso-PGF partially mediated the positive associations between PCBs and FPG values, with the mediated proportions ranged from 3.20 to 12.93%. In conclusion, our results suggested that serum PCBs were positively related with increased oxidative stress, FPG values, and the risk of diabetes among a general Chinese population. Serum PCBs mixture had positive overall effect on the risk of diabetes. Lipid peroxidation partly mediated the FPG elevation induced by PCB exposure.
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http://dx.doi.org/10.1016/j.envpol.2022.119660DOI Listing
June 2022

Talaromarins A-F: Six New Isocoumarins from Mangrove-Derived Fungus TGGP35.

Mar Drugs 2022 May 27;20(6). Epub 2022 May 27.

Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China.

Six new isocoumarin derivative talaromarins A-F (-), along with 17 known analogues (-), were isolated from the mangrove-derived fungus (Eurotiales: Trichocomaceae) TGGP35. Their structures were identified by detailed IR, UV, 1D/2D NMR and HR-ESI-MS spectra. The absolute configurations of new compounds were determined by the modified Mosher's method and a comparison of their CD spectra with dihydroisocoumarins described in the literature. The antioxidant, antibacterial, anti-phytopathogenic and inhibitory activity against α-glucosidase of all the isolated compounds were tested. Compounds -, - and - showed similar or better antioxidant activity than the IC values ranging from 0.009 to 0.27 mM, compared with the positive control trolox (IC = 0.29 mM). Compounds , , and exhibited strong inhibitory activities against α-glucosidase with IC values ranging from 0.10 to 0.62 mM, while the positive control acarbose had an IC value of 0.5 mM. All compounds showed no antibacterial or anti-phytopathogenic activity at the concentrations of 50 μg/mL and 1 mg/mL, respectively. These results indicated that isocoumarins will be useful to developing antioxidants and as diabetes control agents.
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http://dx.doi.org/10.3390/md20060361DOI Listing
May 2022

Efficacy of hematopoietic stem cell transplantation in the treatment of children with non-severe aplastic anemia.

Pediatr Transplant 2022 Jun 23:e14340. Epub 2022 Jun 23.

Hematology Center, Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline of Pediatrics (Capital Medical University), Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

Background: Non-severe aplastic anemia is more likely to develop into severe aplastic anemia, and there is no widely accepted treatment plan at present. Hematopoietic stem cell transplantation might be a new therapeutic strategy.

Methods: Retrospectively analyzed 32 patients with non-severe aplastic anemia who underwent hematopoietic stem cell transplantation from September 2007 to September 2020, and the 5-year estimated overall survival rate and the incidence of graft-versus-host disease were analyzed to evaluate the efficacy and safety of hematopoietic stem cell transplantation in the treatment of pediatric non-severe aplastic anemia.

Results: Thirty-two patients who underwent transplantation, 29 patients (90.6%) survived, 3 patients (9.4%) died. The incidence of acute graft-versus-host disease was 51.6% (16/31), including 15 cases (48.4%) of grade I-II and 1 case (3.2%) of grade III-IV. The incidence of chronic graft-versus-host disease was 38.7% (12/31). The 5-year overall survival rate was 91.8%.

Conclusions: Hematopoietic stem cell transplantation is a practicable, safe, and effective treatment option for non-severe aplastic anemia pediatric patients who are suitable for transplant.
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http://dx.doi.org/10.1111/petr.14340DOI Listing
June 2022

A pregnant woman with congenital heart disease complicated by endocarditis.

Eur Heart J Case Rep 2022 Jun 6;6(6):ytac220. Epub 2022 Jun 6.

Department of Cardiovascular Ultrasound, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China.

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http://dx.doi.org/10.1093/ehjcr/ytac220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204469PMC
June 2022

A Novel Light Field Image Compression Method Using EPI Restoration Neural Network.

Biomed Res Int 2022 13;2022:8324438. Epub 2022 Jun 13.

School of Mechanical, Electrical & Information Engineering, Putian University, Putian, Fujian 351100, China.

Different from traditional images, light field images record not only spatial information but also angle information. Due to the large volume of light field data brings great difficulties to storage and compression, light field compression technology has attracted much attention. The epipolar plane image (EPI) contains a lot of low rank information, which is suitable for recovering the complete EPI from a part of EPI. In this paper, a light field image coding framework based on EPI restoration neural network has been proposed. Compared with previous algorithms, the proposed algorithm further takes advantage of the inherent similarity in light field images, and the proposed framework has higher performance and robustness. Experimental results show that the proposed method has superior performance compared to the state-of-the-art both in quantitatively and qualitatively.
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http://dx.doi.org/10.1155/2022/8324438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209000PMC
June 2022

CP-25 enhances OAT1-mediated absorption of methotrexate in synoviocytes of collagen-induced arthritis rats.

Acta Pharmacol Sin 2022 Jun 22. Epub 2022 Jun 22.

Institute of Clinical Pharmacology, Anhui Medical University; Key Laboratory of Anti-Inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Centre of Anti-Inflammatory and Immune Medicine, Hefei, 230032, China.

Organic anion transporter 1 (OAT1) plays a major role in mediating the absorption, distribution and excretion of drugs and other xenobiotics in the human body. In this study we explored the OAT1 status in rheumatoid arthritis (RA) patients and arthritic animals and its role in regulating the anti-arthritic activity of methotrexate (MTX). We showed that OAT1 expression was significantly downregulated in synovial tissues from RA patients compared with that in the control patients. In collagen-induced arthritis (CIA) rats, synovial OAT1 expression was significantly decreased compared with the control rats. In synoviocytes isolated from CIA rats, PGE2 (0.003-1.75 μM) dose-dependently downregulated OAT1 expression, resulting in decreased absorption of MTX. Silencing OAT1 in synoviocytes caused a 43.7% reduction in the uptake of MTX. Furthermore, knockdown of OAT1 impaired MTX-induced inhibitory effects on the viability and migration of synoviocytes isolated from CIA rats. Moreover, injection of OAT1-shRNA into articular cavity of CIA rats significantly decreased synovial OAT1 expression and impaired the anti-arthritic action of MTX, while injection of lentivirus containing OAT1 sequences led to the opposite results. Interestingly, we found that paeoniflorin-6'-O-benzene sulfonate (CP-25) upregulated OAT1 expression both in vitro and in vivo and promoted MTX uptake by synoviocytes via regulating OAT1 expression and function. Taken together, OAT1 plays a major role in regulating MTX uptake by synoviocytes and the anti-arthritic activity of MTX. OAT1 is downregulated in RA and CIA rats, which can be improved by CP-25.
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http://dx.doi.org/10.1038/s41401-022-00931-5DOI Listing
June 2022

Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells.

Microbiol Spectr 2022 Jun 22:e0074422. Epub 2022 Jun 22.

Department of Veterinary Medicine, Zhejiang Universitygrid.13402.34, Hangzhou, China.

Severe acute respiratory syndrome coronavirus (SARS-CoV)-2 is responsible for the COVID-19 pandemic that has caused unprecedented loss of life and economic trouble all over the world, though the mechanism of its replication remains poorly understood. In this study, antibodies were generated and used to systematically determine the expression profile and subcellular distribution of 11 SARS-CoV-2 nonstructural replicase proteins (nsp1, nsp2, nsp3, nsp5, nsp7, nsp8, nsp9, nsp10, nsp13, nsp14, and nsp15) by Western blot and immunofluorescence assay. Nsp3, nsp5, and nsp8 were detected in perinuclear foci at different time points, with diffusion and stronger fluorescence observed over time. In particular, colocalization of nsp8 and nsp13 with different replicase proteins suggested viral protein-protein interaction, which may be key to understanding their functions and potential molecular mechanisms. Viral intermediate dsRNA was detected in perinuclear foci as early as 2-h postinfection, indicating the initiation of virus replication. With the passage of time, these perinuclear dsRNA foci became larger and brighter, and nearly all colocalized with N protein, consistent with viral growth over time. Thus, the development of these anti-nsp antibodies provides basic tools for the further study of replication and diagnosis of SARS-CoV-2. The intracellular localization of SARS-CoV-2 replicase nonstructural proteins (nsp) during infection has not been fully elucidated. In this study, we systematically analyzed the expression and subcellular localization of 11 distinct viral nsp and dsRNA over time in SARS-CoV-2-infected cells by using individual antibody against these replicase proteins. The data indicated that nsp gene expression is highly regulated in space and time, which could be useful to understand the function of viral replicases and future development of diagnostics and potential antiviral strategies against SARS-CoV-2.
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http://dx.doi.org/10.1128/spectrum.00744-22DOI Listing
June 2022

Allicin attenuated hepatic ischemia/reperfusion injury in mice by regulating PPARγ-IRAK-M-TLR4 signal pathway.

Food Funct 2022 Jun 22. Epub 2022 Jun 22.

Department of Anesthesiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

: Hepatic ischemia/reperfusion (I/R) injury to the liver is a significant cause of morbidity and mortality following liver surgery, trauma, and hemorrhagic shock. It was reported that allicin, a type of garlic compound, had a protective effect against other hepatic diseases. Allicin's ability to protect against liver injury caused by ischemic reperfusion remains unknown. As a result, we conducted this study to determine allicin's effects and mechanism of action in hepatic I/R injury. : The liver I/R injury model was established by clamping the blood supply to the left and middle liver lobes. Three days prior to the hepatic I/R injury, different concentrations of allicin were gavaged. Then, hepatic function, histological changes, apoptosis markers, oxidative stress, and inflammatory cytokines were measured, and the molecular mechanisms were evaluated using western blot. Another separation experiment used IRAK-M knockout mice and peroxisome proliferator-activated receptor-gamma (PPARγ) inhibitor to deduce the molecular mechanisms. : Pretreatment with allicin prior to hepatic I/R injury reduced liver damage by inhibiting aminotransferase activity and alleviating liver injury. It significantly decreased cell apoptosis, interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) production, and hepatic oxidative stress. Furthermore, this study demonstrated that GW9662 (inhibitor of PPARγ) abrogated allicin's positive effect by inhibiting PPARγ expression while suppressing IRAK-M expression. Thus, the depletion of IRAK-M cannot influence the expression of PPARγ. The down-regulation of PPARγ-IRAK-M disabled the protection of allicin in I/R injury. : Allicin protects against hepatic I/R injury dose-dependent regulation of the PPARγ-IRAK-M-TLR4 signaling pathway, and it may be a potential drug in future clinical treatment.
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http://dx.doi.org/10.1039/d2fo00751gDOI Listing
June 2022

Long noncoding RNA ST8SIA6-AS1 promotes cell proliferation and metastasis in triple-negative breast cancer by targeting miR-145-5p/CDCA3 to inactivate the p53/p21 signaling pathway.

Environ Toxicol 2022 Jun 22. Epub 2022 Jun 22.

Department of Breast Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Background: Triple-negative breast cancer (TNBC), the most aggressive subtype of breast cancer, always exhibits a poor prognosis due to high risk of early recurrence and distant metastasis. Long noncoding RNAs (lncRNAs) have been reported as crucial regulators in breast cancer. However, the functions and action mechanisms of lncRNA ST8SIA6-AS1 in TNBC are largely unknown.

Methods: Quantitative real-time PCR and western blot assays were used to measure the expression levels of different genes and proteins. Cell proliferation ability was monitored by CCK-8, colony forming and flow cytometry assays. Wound healing and transwell assays were performed to evaluate cell migration and invasion. The regulatory mechanisms of ST8SIA6-AS1 in TNBC were confirmed by dual luciferase reporter and RIP assays. A mouse xenograft model was established to investigate the role of ST8SIA6-AS1 in TNBC tumor growth.

Results: ST8SIA6-AS1 displayed a higher expression in TNBC cells. Silencing ST8SIA6-AS1 impaired cell proliferation, cell cycle progression, migration, and invasion in vitro, and slowed tumor growth in vivo. Mechanistically, ST8SIA6-AS1 could facilitate the expression of its target CDCA3 (cell division cycle associated protein 3) and inactivate the p53/p21 signaling by inhibiting miR-145-5p. Moreover, miR-145-5p exerted a tumor-suppressive activity by targeting CDCA3. The tumor-suppressive effects induced by ST8SIA6-AS1 knockdown were abated by the down-regulation of miR-145-5p or the up-regulation of CDCA3.

Conclusion: ST8SIA6-AS1 exerts an oncogenic role in TNBC by interacting with miR-145-5p to up-regulate CDCA3 expression and inactivate the p53/p21 signaling, highlighting ST8SIA6-AS1 as a promising molecular target to combat TNBC.
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http://dx.doi.org/10.1002/tox.23605DOI Listing
June 2022

A rat study model of depression-driven chronic prostatitis by modulating the PI3K/Akt/mTOR network.

Andrologia 2022 Jun 21:e14488. Epub 2022 Jun 21.

Department of Andrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Depression and chronic prostatitis (CP) are two common diseases that affect the human population worldwide. Clinically, it has been demonstrated that andrological patients often simultaneously suffer from depression and CP. Prior investigations have established that depression acts as an independent risk factor for CP. Herein, we explored the correlation between depression and CP using bioinformatics tools and through animal experiments. The potential targets and signalling pathways involved in depression and CP were predicted using bioinformatics tool, while depression in the rat model was established through chronic restraint stress. The expression of the related proteins and mRNA was assessed by Western blotting and real-time fluorescence quantitative reverse transcription-polymerase chain reaction (RT-qPCR). Relative to those in the control rats, the protein contents of PI3K, p-Akt, and p-mTOR were lower in the model rats (p < 0.05). Similarly, the transcript levels of PI3K, Akt, and mTOR was also relatively lower in the model rats (p < 0.05). And PI3K/Akt agonists reduced inflammation in rat prostate tissue, accompanied by significant increases in the transcript and protein expression levels of PI3K, Akt, and mTOR. Thus, we proposed that depression model rats may induce CP as a result of mediation by the negative regulation of the PI3K/Akt/mTOR signalling network.
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http://dx.doi.org/10.1111/and.14488DOI Listing
June 2022

A long-term anti-inflammation markedly alleviated high-fat diet-induced obesity by repeated administrations of overexpressing IL10 human umbilical cord-derived mesenchymal stromal cells.

Stem Cell Res Ther 2022 Jun 17;13(1):259. Epub 2022 Jun 17.

Clinical Stem Cell Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, 210000, China.

Objectives: Obesity is a chronic process and could activate various inflammatory responses, which in turn aggravates obesity and related metabolic syndrome. Here we explored whether long-term inhibition of inflammation could successfully alleviate high-fat diet (HFD)-induced obesity.

Methods: We constructed stable overexpressing interleukin 10 (IL10) human umbilical cord-derived mesenchymal stromal cells (HUCMSCs) which repeatedly were applied to obesity mice with HFD feeding to obtain a long-term anti-inflammation based on the prominent anti-inflammation effects of IL10 and immunomodulatery effects of HUCMSCs. Then we monitored the features of obesity including body weight, serum ALT, AST, and lipids. In addition, glucose homeostasis was determined by glucose tolerance and insulin sensitivity tests. The infiltrated macrophages in adipose tissues and hepatic lipid accumulation were detected, and the expressions of adipogenesis and inflammatory genes in adipose tissues were examined by real-time (RT) PCR and western blot analysis.

Results: Compared with HUCMSCs, IL10-HUCMSCs treatment had much better anti-obesity effects including body weight reduction, less hepatic lipids accumulation, lower amount and size of adipocyte, greater glucose tolerance, less systemic insulin resistance, and less adipose tissue inflammation in HFD feeding mice. Finally, IL10-HUCMSCs could decrease the activation of MAPK JNK of adipose tissue induced by HFD. The inhibition of MAPK JNK signal pathway by a small chemical molecule SP600125 in 3T3-L1 cells, a preadipocyte line, reduced the differentiation of adipocytes and lipid droplet accumulation.

Conclusion: A lasting anti-inflammation based on gene modified stem cell therapy is an effective strategy in preventing diet-induced obesity and obesity-related metabolic syndrome.
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http://dx.doi.org/10.1186/s13287-022-02935-8DOI Listing
June 2022

Efficacy and safety of befotertinib (D-0316) in patients with EGFR T790M mutated non-small cell lung cancer that had progressed after prior EGFR TKI therapy: A phase 2, multicenter, single-arm, open-label study.

J Thorac Oncol 2022 Jun 17. Epub 2022 Jun 17.

Department of Medical Oncology/Chemotherapy, The First Affiliated Hospital of University of Science and Technology of China, Anhui Provincial Hospital, Hefei, China.

Introduction: Befotertinib (D-0316) is a novel, third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI). This study evaluated befotertinib in patients with locally advanced or metastatic non-small cell lung cancer (NSCLC) who developed an EGFR T790M mutation after progression on first- or second-generation EGFR TKI therapy.

Methods: This was a single-arm, open-label, phase 2 study at 49 hospitals across mainland China. Patients with locally advanced or metastatic NSCLC harboring EGFR T790M mutations with disease progression following prior first- or second- generation EGFR TKI therapy received oral befotertinib of 50 mg (cohort A) or 75-100 mg (cohort B) once daily. The primary endpoint was objective response rate (ORR) assessed by an independent review committee (IRC) in intention-to-treat population. This trial is registered with ClinicalTrials.gov, number NCT03861156.

Results: A total of 176 patients and 290 patients were included in cohorts A (50 mg) and B (75-100 mg), respectively. At data-cutoff (August 15, 2021), IRC-assessed ORR was 67.6% (95% confidence interval [CI]: 61.9%-72.9%) in cohort B. The investigator-assessed ORR was 54.0% (95% CI: 46.3%-61.5%) in cohort A and 65.9% (95% CI: 60.1%-71.3%) in cohort B. Investigator-assessed disease control rate was 93.2% (95% CI: 88.4%-96.4%) in cohort A and 94.8% (95% CI: 91.6%-97.1%) in cohort B. Investigator-assessed intracranial ORR was 26.7% (95% CI: 7.8%-55.1%) in cohort A and 57.1% (95% CI: 34.0%-78.2%) in cohort B. The median investigator-assessed progression-free survival (PFS) was 11.0 (95% CI: 9.6-12.5) months in cohort A and 12.5 (95% CI: 11.1-13.8) months in cohort B. The median investigator-assessed intracranial PFS was 16.5 (95% CI: 8.6-not evaluable [NE]) months in cohort A and NE (95% CI: 13.8-NE) in cohort B. The overall survival was immature. Grade 3 or higher treatment-related adverse events and treatment-related serious adverse events occurred in 20.5% and 11.4% of patients in cohort A, and in 29.3% and 10.0% of patients in cohort B, respectively.

Conclusion: Befotertinib of 75-100 mg has satisfying efficacy and manageable toxicity in patients with locally advanced or metastatic NSCLC harboring T790M mutation with resistance to first- or second- generation EGFR TKIs. A phase 3 randomized trial is underway (NCT04206072).
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http://dx.doi.org/10.1016/j.jtho.2022.06.002DOI Listing
June 2022

Reverse homodigital dorsal wraparound flap for reconstruction of distal thumb.

J Plast Surg Hand Surg 2022 Jun 20:1-7. Epub 2022 Jun 20.

Hand Surgery Department, The Second Hospital of Tangshan, Tangshan, P.R. China.

Reconstruction of degloving injury or amputation of distal thumb with no indication of replantation has always been a challenging problem for hand surgeons. In this study, a reverse homodigital dorsal wraparound flap innervated by the dorsal digital nerve was devised to repair degloving injury or amputation of distal thumb in 20 consecutive cases. In nine cases of thumb amputation, we skeletonized the phalanxes of the amputated part as a free cortical bone with Kirschner wires. All flaps survived uneventfully. The radiographs showed bone healing in all the patients of thumb amputation within 6 weeks postoperatively. At final follow-up, the appearance of the reconstructed thumb was acceptable and flap sensation and range of joint motion were satisfactory. This flap is a simple and reliable alternative method for degloving injury or amputation of distal thumb when replantation is impossible and patients refuse to donate tissues from toes. Type of study/level of evidence Therapeutic IV.
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http://dx.doi.org/10.1080/2000656X.2022.2088542DOI Listing
June 2022

Low-temperature growth of ultrathin and epitaxial MoC nanosheets a vapor-liquid-solid process.

Nanoscale 2022 Jun 20. Epub 2022 Jun 20.

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Due to the unique physical and chemical properties, transition metal carbides (TMCs) have many potential applications in the fields of energy conversion and catalysis. Chemical vapor deposition (CVD) is a promising method to synthesize TMCs. However, spatially inhomogeneous supply of transition metal precursor vapor in the normal CVD process generally leads to poor control of the morphology and uniformity of the products. Here, we report a vapor-liquid-solid (VLS) growth process where non-volatile NaMoO is used to act as a liquid precursor for the growth of uniform ultrathin MoC nanosheets on AlO(0001). The morphology of the nanosheets can be controlled by tuning the precursor concentration, annealing time and growth temperature. The roles of Na and the liquid-solid interface in consolidating Mo atoms and promoting the epitaxial growth of MoC nanosheets are demonstrated. Furthermore, we show that the liquid-solid interface can cause the crystalline phase transition of MoC nanosheets through verification experiments.
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http://dx.doi.org/10.1039/d2nr02389jDOI Listing
June 2022

Uncorrected near vision acuity and associated factors in a coastal province in southern China: the Fujian eye cross sectional study.

Ann Transl Med 2022 May;10(10):565

Fujian Provincial Key Laboratory of Ocular Surface and Corneal Diseases, Xiamen, China.

Background: Near vision (NV) is essential to visual quality of individuals, and could be affected by different factors and changed gradually with the development of society. An update estimates is needed. Our study aims to investigate the age-trends in and sociodemographic characteristics associated with uncorrected near vision acuity (UNVA) in people ≥50 years in southern China.

Methods: A population-based, cross-sectional survey on the eye health status of residents in both inland and coastal areas of Fujian Province, southern China was performed by Eye Institute and Affiliated Xiamen Eye Center of Xiamen University. People aged ≥50 years (10,044 subjects) in Fujian province were recruited according to the cluster sampling design by Fujian eye cross sectional study (FJES) group. The contents of the questionnaire survey included age, gender, education, occupation and other socioeconomic status. UNVA and slit lamp examination were performed for the participants in the field survey. Analysis of variance (ANOVA) was applied to compare the mean among groups of normally distributed parameters of UNVA and the chi-square (χ) test was used to compare the proportion.

Results: Among the baseline participants, 8,211 (81.8%) attended follow-up examinations. The sample had a mean age of 64.4 years [standard deviation (SD) =8.9], and 4,836 of the participants were female (58.9%). The average UNVA values for males and females were 0.29±0.18 and 0.28±0.17, respectively (P=0.000). UNVA gradually decreased with age and plateaued between 65 and 80 years old. There were significant differences in the mean values of UNVA associated with different occupations (P=0.000). UNVA was significantly different among people with different education levels (P=0.000). The average UNVA in people in coastal areas was 0.28, while that in people in inland areas was 0.29 (P=0.006). People in urban areas appeared to have better UNVA on average (0.29) than those in rural areas (0.27; P=0.000).

Conclusions: After age 50, NV was reduced gradually. Age, gender, education, occupation, income and geographical factors may affect the NV performance of adults, which should be taken into account to achieve a good management of vision quality.
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http://dx.doi.org/10.21037/atm-22-1526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201167PMC
May 2022

Mesenchymal Stem/Stromal Cells in Progressive Fibrogenic Involvement and Anti-Fibrosis Therapeutic Properties.

Front Cell Dev Biol 2022 1;10:902677. Epub 2022 Jun 1.

Department of Neurosurgery, People's Hospital of Zhengzhou University and Henan Provincial People's Hospital, Zhengzhou, China.

Fibrosis refers to the connective tissue deposition and stiffness usually as a result of injury. Fibrosis tissue-resident mesenchymal cells, including fibroblasts, myofibroblast, smooth muscle cells, and mesenchymal stem/stromal cells (MSCs), are major players in fibrogenic processes under certain contexts. Acknowledging differentiation potential of MSCs to the aforementioned other types of mesenchymal cell lineages is essential for better understanding of MSCs' substantial contributions to progressive fibrogenesis. MSCs may represent a potential therapeutic option for fibrosis resolution owing to their unique pleiotropic functions and therapeutic properties. Currently, clinical trial efforts using MSCs and MSC-based products are underway but clinical data collected by the early phase trials are insufficient to offer better support for the MSC-based anti-fibrotic therapies. Given that MSCs are involved in the coagulation through releasing tissue factor, MSCs can retain procoagulant activity to be associated with fibrogenic disease development. Therefore, MSCs' functional benefits in translational applications need to be carefully balanced with their potential risks.
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http://dx.doi.org/10.3389/fcell.2022.902677DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198494PMC
June 2022

Emission characteristics of volatile organic compounds during a typical top-charging coking process.

Environ Pollut 2022 Jun 16;308:119648. Epub 2022 Jun 16.

CAS Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing, 100190, China; Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China. Electronic address:

The emission of volatile organic compounds (VOCs) from coking industry severely reduces air quality. Using both offline and online methods, the emissions of 124 VOCs and non-methane hydrocarbon (NMHC) in a typical top-charging coke oven were analyzed during the coking process (emissions form the coke oven flue gas, charging, pushing, coke dry quenching, and topside of the coke oven). The concentrations of VOCs in coke oven flue gas and exhaust gas during charging were the highest, which reached 98.2 mg/m and 136.6 mg/m, respectively. This was followed by the concentrations of exhaust gases sourced from the topside of the coke oven, pushing, and coke dry quenching, which were 12.0 mg/m, 1.8 mg/m, and 0.8 mg/m, respectively. The main components of VOCs for the different exhaust emission sources were significantly different. The ozone formation potentials (OFPs) of coke oven flue gas and exhaust gas during charging were the largest, and unsaturated hydrocarbons such as alkenes and benzenes were the main source of ground-level ozone. These data can support researchers in developing adsorption, catalytic oxidation, and other technologies for the removal of VOCs generated by the coking process.
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http://dx.doi.org/10.1016/j.envpol.2022.119648DOI Listing
June 2022

Molecular cloning, expression and appetite regulation function of adiponectin in Siberian sturgeon (Acipenser baerii).

Int J Biol Macromol 2022 Jun 15;214:360-369. Epub 2022 Jun 15.

Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211 # Huimin Road, Chengdu 610000, China. Electronic address:

Adiponectin (AdipoQ) as an adipocytokine has the potential to regulate feeding behavior, but the information about adipoq in fish is limited. In this study, Siberian sturgeon adiponectin (Ssadipoq) gene was cloned encoding 264 amino acids. The amino acid identity of SsAdipoQ was low compared with that of mammals, birds, amphibians and teleost fishes. The expression of Ssadipoq in the hypothalamus was significantly decreased at 1 h and 3 h post feeding, and increased after 15-day fasting. The mature domain of AdipoQ (fAd) was inserted into expression vector pET32a and successfully expressed in Escherichia coli BL21 (DE3) after stimulated by isopropyl-β-d-thiogalactoside. Food intake at 1 h and 3 h post treatment with SsfAd protein decreased significantly (P < 0.05). The mRNA expression of pyy and cck in the valvula intestine was promoted and hypothalamic npy, agrp and pomc mRNA expression were inhibited after treatment with SsfAd protein. Furthermore, hypothalamic ampk subunits expression was associated with peripheral SsfAd treatment. In summary, present study indicate that SsfAd plays an important role in the regulation of food intake and appetite signals in Siberian sturgeon, which provides a basis for further study application of prokaryotic AdipoQ in feeding behavior regulation.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.06.097DOI Listing
June 2022

Highly efficient and rapid purification of organic dye wastewater using lignin-derived hierarchical porous carbon.

J Colloid Interface Sci 2022 Jun 7;625:158-168. Epub 2022 Jun 7.

State Key Laboratory of Pulp and Paper Engineering, Plant Fiber Material Science Research Center, South China University of Technology, Guangzhou 510640, China.

Coating manufacturing, textile processing, and plastic industry have led to dramatical release levels of hazardous organic dye pollutants threatening public health and the environment. To solve this problem, porous carbon materials are being developed following with the United Nations initiative on water purification. However, conventional porous carbon materials face many challenges, such as limited removal rates, low adsorption capacity, and high chemicals consumption, hampering their large-scale utilization in dye wastewater treatment. Herein, we demonstrate a high-performance lignin-derived hierarchical porous carbon (LHPC) material directly prepared from renewable lignin through a low-cost activation procedure. The large specific surface area (1824 m/g) enables the rapid and effective adsorption of organic dyes. Therefore, the LHPC exhibits an ultrahigh adsorption ability (1980.63 mg/g) and removal rate (99.03% in 10 min) for Azure B, superior to that of other adsorbents. Additionally, the LHPC adsorbent, organic dyes, eluting agent, and water all can be recycled and reused in a designed close-looped system. Its high removal ability and rate, strong retrievability, low-cost and scalable production combined with high dyes adsorption universality, positions our LHPC as a promising commercial adsorbent candidate for the purification of harmful organic dye wastewater, especially for heavily polluted area with an insistent demand for clear water.
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http://dx.doi.org/10.1016/j.jcis.2022.06.019DOI Listing
June 2022

CircHIPK3 prevents chondrocyte apoptosis and cartilage degradation by sponging miR-30a-3p and promoting PON2.

Cell Prolif 2022 Jun 18:e13285. Epub 2022 Jun 18.

Department of Orthopaedics, The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, China.

Osteoarthritis (OA) is a common joint disease featured by the deterioration of articular cartilage and chondrocyte death. Emerging evidence has indicated that circular RNAs (circRNAs) play an essential role in OA progress. Here, we found that the expression of circHIPK3 was significantly decreased in human and mouse OA cartilage. Knocking down circHIPK3 increased apoptosis and intracellular ROS level in HC-a chondrocytes. We performed proteomic studies and identified that circHIPK3 regulated chondrocyte apoptosis through the mitochondrial pathway. Results of JC-1 staining and western blot further confirmed that mitochondrial outer membrane permeabilization was promoted in HC-a chondrocytes transfected by circHIPK3 siRNA. In terms of mechanism, we showed that PON2 functioned as a potential target of circHIPK3 to regulate chondrocyte apoptosis. Moreover, we revealed that circHIPK3 interacted with miR-30a-3p to regulate PON2 expression in chondrocytes. Taken together, our findings suggested that circHIPK3 regulated chondrocyte apoptosis by mitochondrial pathway, and targeting the circHIPK3/miR-30a-3p/PON2 axis might be a potential strategy for OA treatment.
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http://dx.doi.org/10.1111/cpr.13285DOI Listing
June 2022

PDGFA-associated protein 1 is a novel target of c-Myc and contributes to colorectal cancer initiation and progression.

Cancer Commun (Lond) 2022 Jun 18. Epub 2022 Jun 18.

National Translational Science Center for Molecular Medicine & Department of Cell Biology, Fourth Military Medical University, Xi'an, Shaanxi, 710032, P. R. China.

Background: The mechanism underlying colorectal cancer (CRC) initiation and progression remains elusive, and overall survival is far from satisfactory. Previous studies have shown that PDGFA-associated protein 1 (PDAP1) is upregulated in several cancers including CRC. Here, we aimed to identify the cause and consequence of PDAP1 dysregulation in CRC and evaluate its role as a potential therapeutic target.

Methods: Multi-omics data analysis was performed to identify potential key players in CRC initiation and progression. Immunohistochemistry (IHC) staining was applied to determine the expression pattern of PDAP1 in CRC tissues. Pdap1 conditional knockout mice were used to establish colitis and CRC mouse models. RNA sequencing, a phosphoprotein antibody array, western blotting, histological analysis, 5-bromo-2'-deoxyuridine (BrdU) incorporation assay, and interactome analysis were applied to identify the underlying mechanisms of PDAP1. A human patient-derived xenograft (PDX) model was used to assess the potential of PDAP1 as a therapeutic target.

Results: PDAP1 was identified as a potential key player in CRC development using multi-omics data analysis. PDAP1 was overexpressed in CRC cells and correlated with reduced overall survival. Further investigation showed that PDAP1 was critical for the regulation of cell proliferation, migration, invasion, and metastasis. Significantly, depletion of Pdap1 in intestinal epithelial cells impaired mucosal restitution in dextran sulfate sodium salt-induced colitis and inhibited tumor initiation and growth in colitis-associated cancers. Mechanistic studies showed that c-Myc directly transactivated PDAP1, which contributed to the high PDAP1 expression in CRC cells. PDAP1 interacted with the juxtamembrane domain of epidermal growth factor receptor (EGFR) and facilitated EGFR-mitogen-activated protein kinase (MAPK) signaling activation, which resulted in FOS-related antigen 1 (FRA-1) expression, thereby facilitating CRC progression. Notably, silencing of PDAP1 could hinder the growth of patient-derived xenografts that sustain high PDAP1 levels.

Conclusions: PDAP1 facilitates mucosal restitution and carcinogenesis in colitis-associated cancer. c-Myc-driven upregulation of PDAP1 promotes proliferation, migration, invasion, and metastasis of CRC cells via the EGFR-MAPK-FRA-1 signaling axis. These findings indicated that PDAP1 inhibition is warranted for CRC patients with PDAP1 overexpression.
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http://dx.doi.org/10.1002/cac2.12322DOI Listing
June 2022

Microfluidic Sampling and Biosensing Systems for Foodborne and .

Foodborne Pathog Dis 2022 Jun;19(6):359-375

U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia, USA.

Developments of portable biosensors for field-deployable detections have been increasingly important to control foodborne pathogens in regulatory environment and in early stage of outbreaks. Conventional cultivation and gene amplification methods require sophisticated instruments and highly skilled professionals; while portable biosensing devices provide more freedom for rapid detections not only in research laboratories but also in the field; however, their sensitivity and specificity are limited. Microfluidic methods have the advantage of miniaturizing instrumental size while integrating multiple functions and high-throughput capability into one streamlined system at low cost. Minimal sample consumption is another advantage to detect samples in different sizes and concentrations, which is important for the close monitoring of pathogens at consumer end. They improve measurement or manipulation of bacteria by increasing the ratio of functional interface of the device to the targeted biospecies and in turn reducing background interference. This article introduces the major active and passive microfluidic devices that have been used for bacteria sampling and biosensing. The emphasis is on particle-based sorting/enrichment methods with or without external physical fields applied to the microfluidic devices and on various biosensing applications reported for bacteria sampling. Three major fabrication methods for microfluidics are briefly discussed with their advantages and limitations. The applications of these active and passive microfluidic sampling methods in the past 5 years have been summarized, with the focus on and . The current challenges to microfluidic bacteria sampling are caused by the small size and nonspherical shape of various bacterial cells, which can induce unpredictable deviations in sampling and biosensing processes. Future studies are needed to develop rapid prototyping methods for device manufacturing, which can facilitate rapid response to various foodborne pathogen outbreaks.
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http://dx.doi.org/10.1089/fpd.2021.0087DOI Listing
June 2022

Tumor-Derived Exosomes Regulate Apoptosis of CD45EpCAM Cells in Lung Cancer.

Front Immunol 2022 30;13:903882. Epub 2022 May 30.

Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, China.

Lung cancer has the highest mortality rate among human cancers, and the majority of deaths result from metastatic spread. The tumor microenvironment plays an important role in suppressing the immune surveillance and elimination of tumor cells. A few studies have reported the presence of CD45EpCAM double-positive cells in cancer, but the underlying mechanism remains unclear with respect to how these cells originate and their function in cancer biology. In this study, we analyzed 25 lung tumor samples. We confirmed the presence of CD45EpCAM cells in lung cancer, and these cells exhibited higher apoptosis than CD45EpCAM cells. Using co-culture of lung cancer cell-derived exosomes with healthy donor peripheral blood mononuclear cells, we recapitulated CD45EpCAM cell formation and increased apoptosis that occurs in patients with primary lung cancer. Further analysis suggested that microRNAs in lung cancer cell-derived exosomes may alter the gene expression profile of CD45EpCAM cells, resulting in elevated expression and increased apoptosis. To our knowledge, this is the first report of cancer cell-derived exosomes that can inhibit the immune system by promoting immune cell apoptosis.
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http://dx.doi.org/10.3389/fimmu.2022.903882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192438PMC
June 2022
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