Publications by authors named "Bin Li"

5,263 Publications

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A unique stone skipping-like trajectory of asteroid Aletai.

Sci Adv 2022 Jun 24;8(25):eabm8890. Epub 2022 Jun 24.

State Key Laboratory of Lunar and Planetary Sciences, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau.

Meteoroids/asteroids could deposit energy to Earth during their entries, which arouses great concerns. Strewn field, as a product of meteoroids/asteroids breakup, comprehensively reflects the trajectory, dynamics, and physical properties of meteoroids/asteroids. It typically has a length of several to a dozen kilometers. Nevertheless, the recently found massive Aletai irons in the northwest China comprise the longest known strewn field of ~430 kilometers. This implies that the dynamics of Aletai could be unique. Petrographic and trace elemental studies suggest that all the Aletai masses exhibit unique compositions (IIIE anomalous), indicating that they were from the same fall event. Numerical modeling suggests that the stone skipping-like trajectory associated with a shallow entry angle (e.g., ~6.5° to 7.3°) is responsible for Aletai's exceptionally long strewn field if a single-body entry scenario is considered. The stone skipping-like trajectory would not result in the deposition of large impact energy on the ground but may lead to the dissipation of energy during its extremely long-distance flight.
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http://dx.doi.org/10.1126/sciadv.abm8890DOI Listing
June 2022

Bovine Astrovirus-A Comprehensive Review.

Viruses 2022 Jun 2;14(6). Epub 2022 Jun 2.

Heilongjiang Provincial Key Laboratory of the Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Sartu District, Daqing 163319, China.

Bovine astrovirus (BoAstV) is a small non-enveloped virus with a single-stranded positive-sense RNA. In 1978, BoAstV was first found in calf diarrhea fecal samples in the United Kingdom and since then it has been reported in many other countries. It has wide tissue tropism and can infect multiple organs, including the intestine, nerves and respiratory tract. Since BoAstV is prevalent in healthy as well as clinically infected bovines, and is mostly associated with co-infection with other viruses, the pathogenic nature of BoAstV is still unclear. At present, there are no stable passage cell lines available for the study of BoAstV and animal model experiments have not been described. In addition, it has been reported that BoAstV may have the possibility of cross-species transmission. This review summarizes the current state of knowledge about BoAstV, including the epidemiology, evolution analysis, detection methods, pathogenesis and potential cross species transmission, to provide reference for further research of BoAstV.
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http://dx.doi.org/10.3390/v14061217DOI Listing
June 2022

New Terpenoids from Bornm. and Their Cytotoxic Activities.

Molecules 2022 Jun 7;27(12). Epub 2022 Jun 7.

TCM and Ethnomedicine Innovation and Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.

Two new A-ring contracted triterpenoids, madengaisu A and madengaisu B, and one undescribed -kaurane diterpenoid, madengaisu C, along with 20 known compounds were isolated from the roots of Bornm. The structures were elucidated using extensive spectroscopic techniques, including 1D and 2D-NMR, HR-ESI-MS, ECD spectra, IR, and UV analysis. Moreover, all isolated constituents were evaluated for their anti-proliferative activity against RA-FLS cells and cytotoxic activities against the human cancer cell lines Hep-G2, HCT-116, BGC-823, and MCF-7. Ursolic acid and pomolic acid displayed moderate inhibitory activity in RA-FLS cells with IC values of 24.63 ± 1.96 and 25.12 ± 1.97 μM, respectively. Hyptadienic acid and 2,3-dihydroxyolean-12-en-28-oic acid 28---d-glucopyranoside exhibited good cytotoxicity against Hep-G2 cells with IC values of 25.16 ± 2.55 and 17.66 ± 1.82 μM, respectively. In addition, 2,3-dihydroxyolean-13(18)-en-28-oic acid and alphitolic acid were observed to inhibit HCT-116 cells (13.25 ± 1.65 and 21.62 ± 0.33 μM, respectively), while madengaisu B and 2,3-dihydroxyolean-13(18)-en-28-oic acid showed cytotoxic activities against BGC-823 cells with IC values of 24.76 ± 0.94 and 26.83 ± 2.52 μM, respectively, which demonstrated that triterpenes from may serve as therapeutic agents for RA and cancer treatment.
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http://dx.doi.org/10.3390/molecules27123665DOI Listing
June 2022

Development of Droplet Digital PCR Assay for Detection of Seed-Borne and Causing Bacterial Panicle Blight Disease of Rice.

Microorganisms 2022 Jun 15;10(6). Epub 2022 Jun 15.

State Key Laboratory of Rice Biology, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang Province Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, College of Agricultural and Biotechnology, Zhejiang University, Hangzhou 310058, China.

Bacterial panicle blight of rice or bacterial grain rot of rice is a worldwide rice disease. and are the causal agents. The early and accurate detection of seed-borne and is critical for domestic and international quarantine and effective control of the disease. Here, genomic analyses revealed that contains five phylogroups and the BG1 primer pair designed to target the 3'-end sequence of a gene encoding a Rhs family protein is specific to and two phylogroups within . Using the BG1 primer pair, a 138-bp DNA fragment was amplified only from the tested panicle blight pathogens and . An EvaGreen droplet digital PCR (dPCR) assay on detection and quantification of the two pathogens was developed from a SYBR Green real-time quantitative PCR (qPCR). The detection limits of the EvaGreen droplet dPCR on the two pathogens were identical at 2 × 10 colony forming units (CFU)∙mL from bacterial suspensions and 2 × 10 CFU∙seed from rice seeds. The EvaGreen droplet dPCR assay showed 10-fold detection sensitivity of the SYBR Green qPCR and could detect a single copy of the target gene in a 20-μL assay. Together, the SYBR Green qPCR assay allows for routine high-throughput detection of the panicle blight pathogens and the EvaGreen droplet dPCR assay provides a high-sensitive and high-accurate diagnostic method for quarantine of the pathogens.
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http://dx.doi.org/10.3390/microorganisms10061223DOI Listing
June 2022

Facile Solution Process of VO Film with Mesh Morphology for Enhanced Thermochromic Performance.

Materials (Basel) 2022 Jun 10;15(12). Epub 2022 Jun 10.

Department of Chemistry, University College London, London WC1H0AJ, UK.

The fabrication and applications of VO film continue to be of considerable interest due to their good thermochromic performance for smart windows. However, low visible transmittance () and solar modulation efficiency (∆) impede the application of VO film, and they are difficult to improve simultaneously. Here, a facile zinc solution process was employed to control the surface structure of dense VO film and the processed VO film showed enhanced visible transmittance and solar modulation efficiency, which were increased by 7.5% and 9.5%, respectively, compared with unprocessed VO film. This process facilitated the growth of layered basic zinc acetate (LBZA) nanosheets to form mesh morphology on the surface of VO film, where LBZA nanosheets enhance the visible transmittance as an anti-reflection film. The mesh morphology also strengthened the solar modulation efficiency with small caves between nanosheets by multiplying the times of reflection. By increasing the zinc concentration from 0.05 mol/L to 0.20 mol/L, there were more LBZA nanosheets on the surface of the VO film, leading to an increase in the solar/near-infrared modulation efficiency. Therefore, this work revealed the relationship between the solution process, surface structure, and optical properties, and thus can provide a new method to prepare VO composite film with desirable performance for applications in smart windows.
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http://dx.doi.org/10.3390/ma15124129DOI Listing
June 2022

Roles of Indulgence versus Restraint Culture and Ability to Savor the Moment in the Link between Income and Subjective Well-Being.

Int J Environ Res Public Health 2022 Jun 7;19(12). Epub 2022 Jun 7.

Department of Marketing and Business Law, Loyola Marymount University, Los Angeles, CA 60045, USA.

Over the past few decades, various academic fields have reported contradictory findings regarding whether income is positively or negatively associated with subjective well-being (SWB). To reconcile the inconclusive observations, researchers tend to use various mediators and moderators that could explain why income is more strongly associated with SWB for certain groups of people and why income could be negatively associated with SWB for other groups. This study endeavored to first test additional mediation roles of financial satisfaction and a sense of control in the link between income and three related yet distinct measures of SWB: life satisfaction, happiness, and emotional well-being (EWB), at both cross-national and individual levels. We further investigated the direct and moderating impacts of national difference in Indulgence versus Restraint (IVR) cultural orientations over two mediating mechanisms (income → financial satisfaction → SWB; income → a sense of control → SWB) using data from 49,097 participants in the 2017-2020 World Values Survey. Additionally, we conducted a moderated mediation analysis of individual difference in ability to savor the moment (ASM) for these two mediating mechanisms based on surveys with 796 respondents from China. Analyses at both national level and individual level confirmed the partial mediating roles of financial satisfaction and a sense of control. We further find a positive, direct impact of IVR on SWB such that people in more indulgence cultures report a higher SWB than those in more restraint cultures. The mediating effects of financial satisfaction were found to be weaker in more indulgence cultures than in more restraint ones, while the mediating effects of a sense of control remain the same. Finally, we find that individuals' ASM does not only directly lead to a higher SWB, but also amplifies the mediation impact of financial satisfaction in the link between income and life satisfaction and in the link between income and EWB. Implications of these findings are offered for public policy makers, employers, and citizens, as well as researchers from different fields.
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http://dx.doi.org/10.3390/ijerph19126995DOI Listing
June 2022

Efficiency of ectopic thymectomy by three surgical approaches in non-thymomatous myasthenia gravis.

Updates Surg 2022 Jun 23. Epub 2022 Jun 23.

Department of Thoracic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, People's Republic of China.

Background: To explore the efficiency of ectopic thymectomy by the three surgical approaches of trans-sternum, right unilateral thoracoscopy and thoracoscopic subxiphoid in patients with non-thymomatous myasthenia gravis.

Methods: 155 consecutive non-thymomatous myasthenia gravis patients who underwent extended thymectomy by 3 approaches including trans-sternum, right unilateral thoracoscopy and thoracoscopic subxiphoid in 1st affiliated hospital of Sun Yat-Sen University from January 2017 to October 2019 were reviewed. Differences of perioperative clinical characteristics in three surgical approaches were analyzed.

Results: Time to onset of myasthenia gravis (early or late) (p = 0.018), blood loss (p < 0.001), duration of operation (p = 0.031), duration and volume of thoracic drainage (p = 0.039 and p = 0.026), length of hospitalization (p = 0.039), the efficiency of ectopic thymectomy (p = 0.037), and the detection rate of ectopic thymus in the second quadrant (p = 0.018) were different among the three surgical approaches. In univariate logistic regression analysis, higher efficiency of ectopic thymectomy were associated with transsternal (OR 2.36, 95% CI 1.32-4.22, p = 0.011) and thoracoscopic subxiphoid approaches (OR 2.07, 95% CI 1.12-3.82, p = 0.033). In the multiple logistic regression analysis, the transsternal approach (OR 2.02, 95% CI 1.10-3.71, p = 0.024) was an independent protective factor for the efficiency of ectopic thymectomy.

Conclusions: Both the right unilateral thoracoscopic and thoracoscopic subxiphoid approaches have advantages over the transsternal approach in short-term postoperative recovery. Transsternal approach is still the best choice for ectopic thymectomy while thoracoscopic subxiphoid approach show the potential as an alternative way.
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http://dx.doi.org/10.1007/s13304-022-01295-5DOI Listing
June 2022

Neuroprotective Effect and Possible Mechanisms of Berberine in Diabetes-Related Cognitive Impairment: A Systematic Review and Meta-Analysis of Animal Studies.

Front Pharmacol 2022 6;13:917375. Epub 2022 Jun 6.

School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Berberine, the main bioactive component of Franch., is widely used in the treatment of diabetes. Previous studies have reported that berberine supplementation may play a multitarget therapeutic role in diabetes-related cognitive impairment (DCI). This systematic review and meta-analysis evaluated the effect and possible mechanisms of berberine in animal models of DCI. Relevant studies were searched through PubMed, Web of Science, Embase, and three Chinese databases (CNKI, Wanfang, and VIP) until March 2022. Twenty studies involving 442 animals were included, and SYRCLE's risk of bias tool was used to assess methodological quality. The statistical analysis was performed using STATA 15.0 to calculate the weighted standard mean difference (SMD) with a 95% confidence interval (CI). The fasting blood glucose (FBG) and Morris water maze test (MWM) were the main outcomes to be analyzed. The overall results showed that berberine could significantly improve FBG, escape latency, the times of crossing the platform, the time spent in the target quadrant, serum insulin, 2hBG of oral glucose tolerance test (OGTT), amyloid β (Aβ), acetylcholinesterase (AChE), oxidative stress, and inflammation levels. The present meta-analysis demonstrated that berberine could not only lower blood glucose levels but also improve learning and memory in DCI animal models, which might involve regulating glucose and lipid metabolism, improving insulin resistance, anti-oxidation, anti-neuroinflammation, inhibiting endoplasmic reticulum (ER) stress; and improving the cholinergic system. However, additional attention should be paid to these outcomes due to the significant heterogeneity.
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http://dx.doi.org/10.3389/fphar.2022.917375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208278PMC
June 2022

Cobalamin-Dependent Radical -Adenosylmethionine Enzymes: Capitalizing on Old Motifs for New Functions.

ACS Bio Med Chem Au 2022 Jun 27;2(3):173-186. Epub 2022 Jan 27.

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.

The members of the radical -adenosylmethionine (SAM) enzyme superfamily are responsible for catalyzing a diverse set of reactions in a multitude of biosynthetic pathways. Many members of this superfamily accomplish their transformations using the catalytic power of a 5'-deoxyadenosyl radical (5'-dAdo•), but there are also enzymes within this superfamily that bind auxiliary cofactors and extend the catalytic repertoire of SAM. In particular, the cobalamin (Cbl)-dependent class synergistically uses Cbl to facilitate challenging methylation and radical rearrangement reactions. Despite identification of this class by Sofia et al. 20 years ago, the low sequence identity between members has led to difficulty in predicting function of uncharacterized members, pinpointing catalytic residues, and elucidating reaction mechanisms. Here, we capitalize on the three recent structures of Cbl-dependent radical SAM enzymes that use common cofactors to facilitate ring contraction as well as radical-based and non-radical-based methylation reactions. With these three structures as a framework, we describe how the Cbl-dependent radical SAM enzymes repurpose the traditional SAM- and Cbl-binding motifs to form an active site where both Cbl and SAM can participate in catalysis. In addition, we describe how, in some cases, the classic SAM- and Cbl-binding motifs support the diverse functionality of this enzyme class, and finally, we define new motifs that are characteristic of Cbl-dependent radical SAM enzymes.
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http://dx.doi.org/10.1021/acsbiomedchemau.1c00051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204698PMC
June 2022

Neobavaisoflavone inhibits allergic inflammatory responses by suppressing mast cell activation.

Int Immunopharmacol 2022 Jun 17;110:108953. Epub 2022 Jun 17.

Henan Key Laboratory of Digestive Organ Transplantation, Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of ZhengZhou University, Henan, China. Electronic address:

Neobavaisoflavone (NBIF), a monomolecular compound extracted from Psoralea corylifolia (Leguminosae), is commonly used in traditional Chinese medicine for multiple purposes. NBIF is known to exert anti-fungal and anti-tumor effects, and promote bone formation. Whether NBIF exhibits anti-allergic effects by regulating mast cell activation remains unclear. Therefore, we designed this study to investigate the anti-allergic effects of NBIF on IgE/Ag-induced mouse bone marrow-derived mast cells and ovalbumin-induced asthma, and the passive systemic anaphylaxis (PSA) reaction in mice. Our results showed that NBIF suppresses the production of leukotriene C4, prostaglandin D2 and inflammatory cytokines, and decreases the degranulation of BMMCs stimulated by IgE/Ag. A thorough investigation ascertained that NBIF suppresses the phosphorylation of mitogen-activated protein kinases, and represses the nuclear factor-κB-related signaling pathway. In addition, the oral administration of NBIF in mice inhibited the IgE-induced PSA reaction in a dose-dependent manner. Overall, we provide new insights into how NBIF regulates the IgE/Ag-mediated signaling pathways. Moreover, our investigation promotes the potential use of NBIF in treating allergy and asthma.
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http://dx.doi.org/10.1016/j.intimp.2022.108953DOI Listing
June 2022

Pearson's principle-inspired hollow metal sulfide for amplified photoelectrochemical immunoassay for disease-related protein.

Biosens Bioelectron 2022 Mar 21:114210. Epub 2022 Mar 21.

Department of Biomedical Sciences, Faculty of Health Sciences, University of Macau, Avenida de Universidade, Taipa, Macau; Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Avenida de Universidade, Taipa, Macau; MoE Frontiers Science Center for Precision Oncology, University of Macau, Avenida de Universidade, Taipa, Macau. Electronic address:

Designing a universal route for rational synthesis of a family of hollow multinary chalcogenide semiconductors for photoelectrochemical biosensors is still facing to the enormous challenges ahead. Herein a template-assisted CuO surface vulcanization and etching through a Pearson's hard and soft acid-base (HSAB) principle was utilized to synthesize hollow CuS photoactive materials for photocurrent detection of prostate-specific antigen (PSA). We initially synthesized cubic CuO and further surface sulfidation and HCl etching to obtain cubic CuS. Inspiringly, stirring of CuS, phosphine (TBP: tributylphosphine) and other metal salts could replace Cu ions to obtain new metal sulfides without changing the framework, size and thickness of the original material. This interesting phenomenon could be explained by HSAB theory, which soft base was favorable for combining soft acid (Cu) to drive Cu out of the framework. Based on the results, HSAB-based reaction system was applied to develop novel photoelectrochemical PSA immunoassay. Polymetallic-doped sulfides (ZnCdS) had better photocurrent response than pure binary sulfides. A copper oxide (CuO)-labeled detection antibody is captured in a microplate along with a sandwich immunoassay in the presence of target PSA. Subsequently, the CuO nanoparticles were dissociated by hydrochloric acid, releasing a large amount of copper ions to participate in the cation exchange reaction with ZnCdS. Such excellent photoelectric conversion materials could sensitively detect target PSA with a wide linear range from 1.0 pg/mL to 10 ng/mL at a limit of detection down to 0.32 pg/mL. Additionally, favorable stability, great anti-interference ability, easy-fabrication, low-cost, and satisfactory accuracy for the analysis of actual samples were acquired. Importantly, the concept of cation exchange reaction can be widely used to synthesize advanced nanomaterials for fabrication of high-efficiency biosensing systems.
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http://dx.doi.org/10.1016/j.bios.2022.114210DOI Listing
March 2022

[Research progress in DIR gene of lignan biosynthesis in medicinal plants].

Zhongguo Zhong Yao Za Zhi 2022 Jun;47(11):2890-2898

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China.

The active components, mainly derived from secondary metabolites of medicinal plants, are the material basis for the efficacy of medicinal plants. Lignans, the secondary metabolites in plants with high bioactivity, are widely distributed in a variety of plant species, and their antiviral, antitumor, antibacterial, and antioxidant activities have been proved in clinical practice. Generally, lignans are diverse in structures with many chiral centers, and most of them are optically active. The biosynthesis of lignans depends on the oxidative coupling reaction through site selection and stereo selection, which impedes synthesized lignans to form racemates, but makes them in a three-dimensional configuration. Dirigent protein(DIR) plays an important role in guiding location selection and stereo selection of lignans in biosynthesis. In vitro studies on lignan biosynthesis have shown that racemic end products are obtained in the absence of DIR proteins, while the products in a three-dimensional configuration can be yielded in the presence of DIR proteins, indicating that DIR proteins play an asymmetric role in the biosynthesis of plant secondary metabolites. The present study reviewed the biolo-gical significance of DIR protein, the cloning of DIR gene, gene structure, catalytic mechanism, and the research progress in Isatis indigotica, Eucommia ulmoides, Forsythia suspensa, Salvia miltiorrhiza, Panax pseudoginseng var. notoginseng, and Schisandra chinensis, which provides a reference for the follow-up research of DIR gene.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20220120.101DOI Listing
June 2022

Rapamycin maintains the primordial follicle pool and protects ovarian reserve against cyclophosphamide-induced damage.

J Reprod Dev 2022 Jun 19. Epub 2022 Jun 19.

Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.

Any abnormal activation of primordial follicles and subsequent depletion can irreversibly diminish the ovarian reserve, which is one of the major chemotherapy-induced adverse effects in young patients with cancer. Herein, we investigated the effects of rapamycin on the activation and development of ovarian follicles to evaluate its fertility-sparing therapeutic value in a cyclophosphamide (CTX)-treated mouse model. Based on ovarian histomorphological changes and follicle counting in 50 SPF female C57BL/6 mice, daily administration of 5 mg/kg rapamycin for 30 days was deemed an ideal dosage and duration for administration in subsequent experiments. Compared with the control group, rapamycin treatment inhibited the activation of quiescent primordial follicles, with no obvious side effects observed. Finally, 48 mice were randomly divided into four groups: control, rapamycin-treated, cyclophosphamide-treated, and rapamycin intervention. Body weight, ovarian histomorphological changes, number of primordial follicles, DDX4/MVH expression, apoptosis of follicular cells, and expression of apoptosis protease-activating factor (APAF)-1, cleaved caspase 3, and caspase 3 were monitored. Co-administration of rapamycin reduced primordial follicle loss and the development of follicular cell apoptosis, thereby rescuing the ovarian reserve after CTX treatment. On analyzing the mTOR signaling pathway, we observed that rapamycin significantly decreased CTX-mediated overactivation of mTOR and its downstream molecules. These findings suggest that rapamycin exhibits potential as an ovarian-protective agent that could maintain the ovarian primordial follicle pool and preserve fertility in young female patients with cancer undergoing chemotherapy.
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http://dx.doi.org/10.1262/jrd.2022-001DOI Listing
June 2022

miR-107 attenuates sepsis-induced myocardial injury by targeting PTEN and activating the PI3K/AKT signaling pathway.

Cells Tissues Organs 2022 Jun 17. Epub 2022 Jun 17.

Sepsis is a public health problem worldwide. This study investigated the mechanism of miR-107 on sepsis-induced myocardial injury. Sepsis rat models were established by cecal ligation and puncture, and the cell model was established using lipopolysaccharide (LPS)-induced cardiomyocytes. Cardiac function indexes of rats were measured using echocardiography. Pathological changes in the rat myocardium were observed using histological staining. Expressions of miR-107 in the serum of rats and cardiomyocyte were detected after the treatment of miR-107 mimic or/and pcDNA3.1-PTEN, followed by assessment of cell cycle, proliferation, and apoptosis. Binding sites of miR-107 and PTEN were predicted. PTEN, PI3K, p-PI3K, AKT, and p-AKT expressions in LPS-induced cardiomyocytes were measured. miR-107 was significantly downregulated in the serum of CLP rats and LPS-induced cardiomyocytes. miR-107 overexpression remarkably improved cardiac function and histological changes, decreased inflammatory factors, and alleviated the sepsis-induced myocardial injury in rats. In LPS-induced cardiomyocytes, miR-107 overexpression increased cardiomyocyte proliferation, inhibited apoptosis, and enhanced the proportion of cardiomyocytes arrested in S and G2/M phases. miR-107 targeted PTEN. PTEN overexpression partially reversed the inhibition of miR-107 mimic on cardiomyocyte apoptosis. miR-107 overexpression activated the PI3K/AKT pathway by inhibiting PTEN. To conclude, miR-107 activates the PI3K/AKT pathway by inhibiting PTEN, thus attenuating sepsis-induced myocardial injury and LPS-induced cardiomyocyte apoptosis.
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http://dx.doi.org/10.1159/000525476DOI Listing
June 2022

LC-MS-based metabolomics reveals metabolic changes in short- and long-term administration of Compound Danshen Dripping Pills against acute myocardial infarction in rats.

Phytomedicine 2022 Jun 12;104:154269. Epub 2022 Jun 12.

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address:

Background: Mild and systematically improving multiple metabolic disorders was a focused view for Compound Danshen Dripping Pills playing synergistic effects through multiple components and multiple targets. The difference in overall therapeutic effects and endogenous metabolic regulation between short- and long-term administration was still unclear.

Purpose: This study aimed to explore the difference in endogenous metabolic regulation between short- and long-term Compound Danshen Dripping Pills (CDDP) administration against acute myocardial infarction (AMI).

Methods: The model of AMI was induced by ligating the left anterior descending coronary artery. The cardiac protection effects of CDDP were investigated by echocardiography, 1- or 2-week were defined as short- and long-term based on desirable efficacy variability. The entire metabolic changes between short- and long-term administration of CDDP were profiled by UPLC-Q-TOF-MS. In addition, the metabolic regulatory network of CDDP administration against myocardial infarction rats was also compared with those of a typical chemical drug isosorbide 5-mononitrate (ISMN).

Results: After 1- or 2-week continuous oral administration, CDDP could significantly alleviate AMI-induced cardiac dysfunction. By using LC-MS-based metabolomics analyses, we systematically investigated the metabolic profiles of plasma and heart tissue samples at fixed exposure time-points (2 h, 24 h) from AMI rats with CDDP treatment. Most interestingly, global endogenous metabolic changes were observed in cardiac samples collected at different stages post consecutive CDDP administration, fluctuating at 2 and 24 h after 1 week but stabilizing after 2 weeks. The disrupted metabolic pathways such as glycerophospholipid, amino acids, fatty acids, and arachidonic acid metabolism were reconstructed after both short- and long-term CDDP treatment, while taurine and hypotaurine metabolism and purine metabolism contributed to the whole efficacy after long-term CDDP administration.

Conclusion: Long-term CDDP treatment plays prolonged and stable efficacy against AMI compared with short-term treatment by specifically regulating purine and taurine and hypotaurine metabolism and systematically redressing metabolic disorders.
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http://dx.doi.org/10.1016/j.phymed.2022.154269DOI Listing
June 2022

Intestinal dysbacteriosis-propelled T helper 17 cells activation mediate the perioperative neurocognitive disorder induced by anesthesia/surgery in aged rats.

Neurosci Lett 2022 Jul 15;783:136741. Epub 2022 Jun 15.

Department of Anesthesiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu 210009, China. Electronic address:

Background: Perioperative neurocognitive disorders (PND) is a common postoperative disease in elderly patients, but its pathogenesis remains unclear.

Methods: Exploratory laparotomy was performed to establish PND model under sevoflurane anesthesia. 16S rRNA high-throughput sequencing was used to detect the changes of intestinal flora. Antibiotics were used to relatively eliminate intestinal flora before anesthesia/surgery, and behavior tests, such as open field, Y maze, and fear conditioning tests were applied to detect the changes of memory ability. The number of Th17 cells and Foxp3 cells was detected by flow cytometry in the Peyer's patches (PP), mesenteric lymph nodes (MLN), blood and brain. Western blot was used to detect the expression of IL17, IL17RA, IL6 and IL10 in the hippocampus. Immunofluorescence was used to detect the expression of IL17, IL17R and IBA1 (ionized calcium binding adaptor molecule1) in the hippocampus.

Results: Anesthesia/surgery caused intestinal flora imbalance and induced neurocognitive impairment, increased the number of Th17 cells in the PP, MLN, blood and brain, increased the level of IL17, IL17R and inflammatory factors production in the hippocampus. Antibiotics administration before anesthesia/surgery significantly decreased the number of Th17 cells and the level of IL17, IL17R and inflammatory factors production, and improved the memory function. In addition, we found that IL17R was co-labeled with IBA1 in a large amount in the hippocampus through immunofluorescence double-staining.

Conclusion: Our study suggested that intestinal dysbacteriosis-propelled T helper 17 cells activation and IL17 secretion might play an important role in the pathogenesis of PND induced by anesthesia/surgery in aged rats.
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http://dx.doi.org/10.1016/j.neulet.2022.136741DOI Listing
July 2022

[Acupuncture as adjuvant therapy for 32 cases of coronavirus disease 2019].

Zhongguo Zhen Jiu 2022 Jun;42(6):634-8

Beijing Hospital of TCM, Capital Medical University, Beijing 100010.

Objective: To observe the clinical effect of acupuncture on coronavirus disease 2019 (COVID-19) based on the conventional treatment.

Methods: A total of 35 patients with COVID-19 of mild or ordinary type were involved (3 cases dropped off). Acupuncture was applied on the basis of western medicine and Chinese materia medica treatment. Dazhui (GV 14), Fengchi (GB 20), Kongzui (LU 6), Hegu (LI 4), etc. were selected as the main acupoints, the supplementary acupoints and the reinforcing and reducing manipulations were selected according to syndrome differentiation. Acupuncture treatment was given once a day, 5 times a week. On day 3 and day 7 of acupuncture, relief condition of the main symptoms was observed. Before acupuncture and on day 3 and day 7 of acupuncture, efficacy evaluation scale of TCM on COVID-19 (efficacy evaluation scale) score was recorded. The effects of different intervention time of acupuncture on patients' hospitalization time were compared, the understanding of acupuncture treatment of patients discharged from hospital was recorded, the clinical efficacy and safety of acupuncture treatment were evaluated.

Results: On day 3 and day 7 of acupuncture, the symptoms of lung system and non lung system were both relieved; the scores of efficacy evaluation scale were both decreased compared before acupuncture (<0.05), and the efficacy evaluation scale score of day 7 of acupuncture were lower than day 3 of acupuncture (<0.05). The average hospitalization time of patients received early acupuncture was shorter than late acupuncture (<0.05). The total effective rate was 84.4% (27/32) on day 7 of acupuncture, which was higher than 34.4% (11/32) on day 3 of acupuncture (<0.05). During the acupuncture treatment, there were neither adverse reactions in patients nor occupational exposures in doctors. The patients generally believed that acupuncture could promote the recovery of COVID-19 and recommended acupuncture treatment.

Conclusion: On the basis of the conventional treatment, acupuncture can effectively relieve the clinical symptoms in patients with COVID-19, early intervention of acupuncture can accelerate the recovery process. Acupuncture has good safety, clinical compliance and recognition of patients.
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http://dx.doi.org/10.13703/j.0255-2930.20211209-0002DOI Listing
June 2022

Immune Cell Infiltration Characteristics of Pigmented Villous Nodular Synovitis and Prediction of Potential Diagnostic Markers Based on Bioinformatics.

Biomed Res Int 2022 7;2022:8708692. Epub 2022 Jun 7.

Division of Joint Surgery and Sports Medicine, Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, 430000 Hubei, China.

Background: Pigmented villous nodular synovitis (PVNS) is a tumor-like proliferative disease characterized by impairment of daily activities, decreased quality of life, and a high recurrence rate. However, the specific pathological mechanisms are still ill-defined and controversial. The purpose of this study was to define potential diagnostic markers and evaluate immune cell infiltration in the pathogenesis of PVNS.

Method: The expression profile of GSE3698 was reanalyzed in the Gene Expression Omnibus (GEO) database. First, differentially expressed genes (DEGs) were identified using the R package "limma" and analyzed by Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. Next, the DEGs were imported into the STRING database and Cytoscape to construct a protein-protein interaction (PPI) network. Then, cytoHubba and ROC curve analyses were used to determine potential diagnostic biomarkers of PVNS. Finally, we used CIBERSORT to estimate the proportions of 22 immune cell subtypes in PVNS and analyzed the correlation between diagnostic markers and infiltrating immune cells.

Result: We found 139 DEGs (including 93 upregulated genes and 46 downregulated genes). TYROBP, FCER1G, LAPTM5, and HLA-DPB1 were identified as potential diagnostic biomarkers of PVNS. Immune cell infiltration analysis indicated that neutrophils and M2 macrophages might be associated with the genesis and progression of PVNS. Furthermore, our correlation analysis of diagnostic markers and infiltrating immune cells found that TYROBP, FCER1G, LAPTM5, and HLA-DPB1 were positively correlated with M2 macrophage infiltration and that neutrophils, TYROBP, FCER1G, and LAPTM5 were negatively correlated with plasma cell infiltration.

Conclusions: We identified TYROBP, FCER1G, LAPTM5, and HLA-DPB1 as potential diagnostic markers for PVNS and concluded that immune cell infiltration plays an important role in the genesis and progression of PVNS.
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http://dx.doi.org/10.1155/2022/8708692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197622PMC
June 2022

Tumor-Derived Exosomes Regulate Apoptosis of CD45EpCAM Cells in Lung Cancer.

Front Immunol 2022 30;13:903882. Epub 2022 May 30.

Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, China.

Lung cancer has the highest mortality rate among human cancers, and the majority of deaths result from metastatic spread. The tumor microenvironment plays an important role in suppressing the immune surveillance and elimination of tumor cells. A few studies have reported the presence of CD45EpCAM double-positive cells in cancer, but the underlying mechanism remains unclear with respect to how these cells originate and their function in cancer biology. In this study, we analyzed 25 lung tumor samples. We confirmed the presence of CD45EpCAM cells in lung cancer, and these cells exhibited higher apoptosis than CD45EpCAM cells. Using co-culture of lung cancer cell-derived exosomes with healthy donor peripheral blood mononuclear cells, we recapitulated CD45EpCAM cell formation and increased apoptosis that occurs in patients with primary lung cancer. Further analysis suggested that microRNAs in lung cancer cell-derived exosomes may alter the gene expression profile of CD45EpCAM cells, resulting in elevated expression and increased apoptosis. To our knowledge, this is the first report of cancer cell-derived exosomes that can inhibit the immune system by promoting immune cell apoptosis.
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http://dx.doi.org/10.3389/fimmu.2022.903882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192438PMC
June 2022

Integrated analysis of tRNA-derived small RNAs in proliferative human aortic smooth muscle cells.

Cell Mol Biol Lett 2022 Jun 15;27(1):47. Epub 2022 Jun 15.

Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China.

Background: Abnormal proliferation of vascular smooth muscle cells (VSMCs) contributes to vascular remodeling diseases. Recently, it has been discovered that tRNA-derived small RNAs (tsRNAs), a new type of noncoding RNAs, are related to the proliferation and migration of VSMCs. tsRNAs regulate target gene expression through miRNA-like functions. This study aims to explore the potential of tsRNAs in human aortic smooth muscle cell (HASMC) proliferation.

Methods: High-throughput sequencing was performed to analyze the tsRNA expression profile of proliferative and quiescent HASMCs. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the sequence results and subcellular distribution of AS-tDR-001370, AS-tDR-000067, AS-tDR-009512, and AS-tDR-000076. Based on the microRNA-like functions of tsRNAs, we predicted target promoters and mRNAs and constructed tsRNA-promoter and tsRNA-mRNA interaction networks. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to reveal the function of target genes. EdU incorporation assay, Western blot, and dual-luciferase reporter gene assay were utilized to detect the effects of tsRNAs on HASMC proliferation.

Results: Compared with quiescent HASMCs, there were 1838 differentially expressed tsRNAs in proliferative HASMCs, including 887 with increased expression (fold change > 2, p < 0.05) and 951 with decreased expression (fold change < ½, p < 0.05). AS-tDR-001370, AS-tDR-000067, AS-tDR-009512, and AS-tDR-000076 were increased in proliferative HASMCs and were mainly located in the nucleus. Bioinformatics analysis suggested that the four tsRNAs involved a variety of GO terms and pathways related to VSMC proliferation. AS-tDR-000067 promoted HASMC proliferation by suppressing p53 transcription in a promoter-targeted manner. AS-tDR-000076 accelerated HASMC proliferation by attenuating mitofusin 2 (MFN2) levels in a 3'-untranslated region (UTR)-targeted manner.

Conclusions: During HASMC proliferation, the expression levels of many tsRNAs are altered. AS-tDR-000067 and AS-tDR-000076 act as new factors promoting VSMC proliferation.
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http://dx.doi.org/10.1186/s11658-022-00346-4DOI Listing
June 2022

Lower Expression of TWEAK is Associated with Poor Survival and Dysregulate TIICs in Lung Adenocarcinoma.

Dis Markers 2022 6;2022:8661423. Epub 2022 Jun 6.

Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.

Background: Lung cancer remains the leading cause of cancer death worldwide, and the most subtype is lung adenocarcinoma (LUAD). Tumor-infiltrating immune cells (TIICs) greatly impact the prognosis of LUAD. Tumor necrosis factor-like weak inducer of apoptosis (TWEAK), signal via its receptor fibroblast growth factor-inducible 14 (Fn14), dysregulates immune cell recruitment within tumor environment, thus promoting the progression of autoimmune diseases and cancer. We aimed to explore its role in LUAD.

Methods: The expression level of TWEAK was explored in Tumor Immune Estimation Resource 2.0 (TIMER2.0) and Oncomine databases. The Tumor Immune Dysfunction and Exclusion (TIDE) and Lung Cancer Explorer (LCE) databases were applied to evaluate the survival in correlation to TWEAK expression. TIICs were assessed with TIMER2.0 and TIDE datasets. The expression of TWEAK protein was detected in LUAD cell lines and also in tissue samples from LUAD patients via western blotting or combination with immunochemistry.

Results: Our results showed that TWEAK was downregulated in LUAD tumors compared to normal tissues in TIMER2.0, Oncomine, cell lines, and clinical specimens. Poor survival was uncovered in lower TWEAK expression of LUAD patients in LCE (meta - HR = 0.84 [95% CI, 0.76-0.92]) and TCGA (Continuous Z = -1.97, = 0.0486) and [email protected] (Continuous Z = -4.25, = 2.12e - 5) in TIDE. Multiple tumor-infiltrating immune cells (TIICs) were found closely correlated with TWEAK expression in LUAD, especially hematopoietic stem cell (Rho = 0.505, = 2.78e - 33), common lymphoid progenitor (Rho = -0.504, = 3.79e - 33), and myeloid-derived suppressor cells (MDSCs) (Rho = -0.615, = 1.36e - 52).

Conclusion: Lower level of TWEAK was linked with poor survival and aberrant recruitment and phenotype of TIICs in LUAD, which might motivate immune escape and weaken the effects of immunotherapy.
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http://dx.doi.org/10.1155/2022/8661423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192298PMC
June 2022

Simultaneously qualitative and quantitative analysis of the representative components in Kadsura heteroclita stem by UHPLC-Q-Orbitrap HRMS.

J Food Drug Anal 2021 Jun 15;29(2):319-363. Epub 2021 Jun 15.

School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Yantai 264005, China.

Kadsura heteroclita (Roxb) Craib stem (KHS) is a medicinal plant used for the treatment of rheumatism arthritis diseases in Tujia ethnomedicine. Thus far, the complex chemical compositions in KHS are not clear, and the levels of the major compounds in KHS are not well understood. In this study, a novel UHPLC-Q-Orbitrap HRMS method was established for the simultaneous quali-quantitative analysis of KHS. A total of 204 compounds were identified, including triterpenoids, lignans, sesquiterpenes, fatty acids, phenolic acids, and flavonoids, more than 100 of which were first discovered in KHS. Using the same method, 12 representative bioactive components were successfully quantified. The method was fully validated by linearity, LOD, LOQ, precision, stability, recovery, and matrix effects, and it was applied to quantify the 12 representative compounds in 4 batches of KHS. As this method enables retrospective data analysis and has no upper limit to the number of analytes in a single run, it can be applied to quantify more active components of KHS in the future.
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http://dx.doi.org/10.38212/2224-6614.3348DOI Listing
June 2021

CREB1 contributes colorectal cancer cell plasticity by regulating lncRNA CCAT1 and NF-κB pathways.

Sci China Life Sci 2022 Jun 10. Epub 2022 Jun 10.

MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.

The CREB1 gene encodes an exceptionally pleiotropic transcription factor that frequently dysregulated in human cancers. CREB1 can regulate tumor cell status of proliferation and/or migration; however, the molecular basis for this switch involvement in cell plasticity has not fully been understood yet. Here, we first show that knocking out CREB1 triggers a remarkable effect of epithelial-mesenchymal transition (EMT) and leads to the occurrence of inhibited proliferation and enhanced motility in HCT116 colorectal cancer cells. By monitoring 45 cellular signaling pathway activities, we find that multiple growth-related pathways decline significantly while inflammatory pathways including NF-κB are largely upregulated in comparing between the CREB1 wild-type and knocked out cells. Mechanistically, cells with CREB1 knocked out show downregulation of MYC as a result of impaired CREB1-dependent transcription of the oncogenic lncRNA CCAT1. Interestingly, the unbalanced competition between the coactivator CBP/p300 for CREB1 and p65 leads to the activation of the NF-κB pathway in cells with CREB1 disrupted, which induces an obvious EMT phenotype of the cancer cells. Taken together, these studies identify previously unknown mechanisms of CREB1 in CRC cell plasticity via regulating lncRNA CCAT1 and NF-κB pathways, providing a critical insight into a combined strategy for CREB1-targeted tumor therapies.
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http://dx.doi.org/10.1007/s11427-022-2108-xDOI Listing
June 2022

Current Status of Chinese Medical Students' Professional Identity After COVID-19 and the Factors That Influence It.

Front Psychol 2022 25;13:816767. Epub 2022 May 25.

First Affiliated Hospital of Gannan Medical University, Ganzhou, China.

This study aimed to explore the current situation regarding medical students' professional identity after COVID-19 in China, as well as the factors that influence it. Questionnaire compiled by Fujian Medical University and self-designed were used, and participators were from one Medical University in Jiangxi Province, a central city of China. Results showed that the professional identity of medical students was upper middle level and the professional attitude was generally positive. There was a significant sex difference in terms of value ( = 2.057, < 0.05) which the scores for boys were higher than girls, whereas the scores for girls were higher than boys when it came to aspects such as professional environment ( = -3.918, < 0.001) and professional cognition ( = -3.855, < 0.001). There was a significant difference in the sense of professional identity between people with and without siblings ( = 2.264, < 0.05). The scores of students who participated in prevention and control of the epidemic were significantly higher than those who did not ( = 2.267, < 0.01). Professional identity decreased gradually in related to higher grades, but it increased at the graduate stage; the grade [ = 10.302, < 0.001] and majors [ = 5.718, < 0.01] differences were significant. Factors such as family members' influence, attitude toward occupation, grades, major, registered residence, and college choosing were the main factors that influenced medical students' professional identity. Overall, the medical students' professional identity needs to be further strengthened in the post COVID-19, it should be increased education regarding career development and planning.
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http://dx.doi.org/10.3389/fpsyg.2022.816767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174680PMC
May 2022

Efficient Protein-Protein Couplings Mediated by Small Molecules under Mild Conditions.

Angew Chem Int Ed Engl 2022 Jun 12. Epub 2022 Jun 12.

Nankai University, college of chemistry, CHINA.

Reactions of protein-protein couplings under physiological conditions without impact on the three-dimensional structures of proteins are highly demanding. The combination effect of phenylsulfonyl and aldehyde in 5-fluoro-4(phenylsulfonyl)-picolinaldehyde (FPPA) results in high reactivity of fluorine for free thiols. In FPPA, the higher reactivity of fluorine than phenylsulfonyl for free thiols, and it leads to the first quantitative nucleophilic substitution and the subsequent reactions either via selective substitution of phenylsulfonyl by an additional thiol or cyclization of aldehyde with a 1, 2-aminothiol molecule. The FPPA mediated protein-protein coupling proceeds efficiently under mild conditions, resulting in stable protein conjugates. This coupling method has negligible 3D structural perturbations on the target proteins, and it produces overall intact, nearly traceless and native structural folds of proteins. It is also highly suitable for segmentally isotopic labeling of multi-domain proteins.
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http://dx.doi.org/10.1002/anie.202205597DOI Listing
June 2022

Altered non-coding RNA profiles and potential disease marker identification in peripheral blood mononuclear cells of patients with NMOSD.

Int Immunopharmacol 2022 Aug 9;109:108899. Epub 2022 Jun 9.

Department of Neurology, The Second Hospital of Hebei Medical University, City Shijiazhuang 050000, Province Hebei, PR China; Key Laboratory of Neurology of Hebei Province, The Second Hospital of Hebei Medical University, City Shijiazhuang 050000, Province Hebei, PR China. Electronic address:

Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory demyelination disorder, and dysregulation of RNAs contributes to its pathogenesis. We aimed to reveal the expression profiles of RNAs, including messenger RNA (mRNA), circular RNA (circRNA) and long non-coding RNA (lncRNA), in the peripheral blood mononuclear cells (PBMCs) of patients with NMOSD. Seven NMOSD patients and seven healthy controls (HCs) were enrolled in the competitive endogenous RNA (ceRNA) microarray analysis. Bioinformatics analysis was then performed on the microarray data. Selected RNAs were validated by RT-qPCR. Differentially expressed (DE) RNA profiles of patients and HCs were related to NK cell mediated cytotoxicity, the IL-17 signaling pathway, and the B cell receptor signaling pathway. Moreover, DE non-coding RNAs (DE ncRNAs) including DE circRNAs and DE lncRNAs, may participate in the transforming growth factor beta (TGF-β) signaling pathway, leukocyte migration and neutrophil chemotaxis. Immune cell infiltration analysis showed that the abundance of M1 macrophages and plasma cells significantly increased, while that of M2 macrophages significantly decreased in the NMOSD group. Finally, through RT-qPCR validation, lnc-HELZ-7:1 (95% confidential interval of area under curve [95%CI of AUC] = 0.6633-1.0000), ring finger protein-LIM domain interacting (RLIM; 95%CI of AUC = 0.6980-1.0000), and hsa_circ_0026993 (95%CI of AUC = 0.7550-1.0000) could discriminate NMOSD from HCs by receiver operating characteristic curve analysis. To our knowledge, this is the first study to preliminarily investigate the RNA profiles, especially circRNA profiles in PBMCs of NMOSD patients from North China. We identified lnc-HELZ-7:1, RLIM, and hsa_circ_0026993 as the potential disease markers for NMOSD.
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http://dx.doi.org/10.1016/j.intimp.2022.108899DOI Listing
August 2022

Facile Fabrication of Cellulose Nanofibrils/Chitosan Beads as the Potential pH-Sensitive Drug Carriers.

Polymers (Basel) 2022 Jun 4;14(11). Epub 2022 Jun 4.

CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.

It is highly desirable to develop a safe, highly efficient, and biodegradable drug carrier with an enhanced drug transport efficiency. Cellulose nanofibrils (CNF) and chitosan (CS) composite hydrogels are promising candidate carriers with biological compatibility and non-cytotoxicity. Herein, the CNF/CS composite beads were prepared by dissolving cellulose and CS in LiBr molten salt hydrate and regenerating in ethanol. This preparation method is facile and efficient, and the obtained porous CNF/CS beads with the weight ratio of 8:2 exhibited a large specific surface area, uniform micro-nano-sized pores, strong mechanical property, and water absorption-resistance. Moreover, these beads as drug (tetracycline hydrochloride, TH) carriers showed a higher encapsulation efficiency (47.4%) at the TH concentration of 5 mg/mL in 24 h, and a higher drug loading rate (12.0%) than pure CNF and other CNF/CS beads prepared with different ratios. In addition, the TH releasing behavior of CNF/CS (8:2) beads fitted well into the zero-order, first-order, and Higuchi models under an acid condition, indicating that the drug release of these pH-sensitive beads was mainly affected by drug concentration under an acid condition. Therefore, these CNF/CS beads have great potential to be used as drug carriers for medical applications.
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http://dx.doi.org/10.3390/polym14112286DOI Listing
June 2022

LncRNA JPX Promotes Esophageal Squamous Cell Carcinoma Progression by Targeting miR-516b-5p/VEGFA Axis.

Cancers (Basel) 2022 May 31;14(11). Epub 2022 May 31.

Department of Thoracic Surgery, Section of Esophageal Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China.

Long non-coding RNAs (lncRNAs) are reported act as important regulators in various types of cancer. LncRNA JPX was identified as an oncogenic regulator in lung cancer. However, the function of JPX in the progression of esophageal squamous cell carcinoma (ESCC) remains unclear. In the present study, we found JPX was highly expressed in esophageal tissue from ESCC patients. Functional assays demonstrated that JPX promoted ESCC cell proliferation, migration, and invasion in vitro, and accelerated tumor growth in vivo. Mechanistically, the results showed that JPX functioned as a sponge of miR-516b-5p, which targeted vascular endothelial growth factor A (VEGFA) in ESCC cells. Interactions between miR-516b-5p and JPX or VEGFA were confirmed by luciferase reporter assays. Inhibition of JPX significantly attenuated the cell growth and mobility ability of ESCC cells in vitro. In addition, overexpression of miR-516b-5p abrogated JPX-enhanced proliferation, migration, invasion, and angiogenesis of ESCC cells. Our study demonstrated that JPX played an important role in promoting ESCC progression via the miR-516b-5p/VEGFA pathway, which might serve as a promising novel diagnostic biomarker and therapeutic target for ESCC in clinic.
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http://dx.doi.org/10.3390/cancers14112713DOI Listing
May 2022

Preparation of Iron Carbon Composite Material by Extracting Iron from Bauxite Residue and Its Adsorption of Heavy Metal Cd(II).

Bull Environ Contam Toxicol 2022 Jun 9. Epub 2022 Jun 9.

Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China.

An effective method of iron extraction from bauxite residue was explored, and iron was used to prepare iron carbon composite material, which have a good adsorption effect on the heavy metal cadmium. After acid washing, acid leaching, Fe(III) reduction and ferrous oxalate decomposition, FeSO·HO(RM) was successfully extracted from bauxite residue, and the iron loss was only 4.35%. FexOy-BC(RM) nanocomposite materials were prepared by loading FeSO·HO(RM) onto walnut shell biochar (BC) (a kind of agricultural and forestry waste) by an in situ reduction and oxidation method. The results showed that the adsorption effect of FexOy-BC(RM) on Cd(II) was better than that of commercial FexOy-BC. XPS, TEM, SEM characterization analysis showed that FexOy-BC(RM) immobilized Cd(II) by adsorption, complexation, etc.to achieve a highly efficient adsorption of heavy metal Cd(II) in wastewater.
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http://dx.doi.org/10.1007/s00128-022-03539-xDOI Listing
June 2022

[Research on Maintenance Management of Equipment of Magnetic Resonance Imaging Based on RAM].

Zhongguo Yi Liao Qi Xie Za Zhi 2022 May;46(3):350-354

Department of Medical Equipment, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, 201306.

Objective: To optimize the maintenance quality management of MRI equipment and ensure the quality and safety of its clinical use.

Methods: The data of failure time and repair time of a MRI equipment in three years were collected by magnetic resonance repair report system, and then the reliability, availability and maintainability(RAM) were studied and analyzed.

Results: The results of reliability analysis showed that the communication module was the key subsystem of the MRI equipment. The results of usability analysis showed that RF module was a key subsystem of MRI equipment. Maintainability results showed that the proportion of the MRI equipment not fully utilized due to maintenance-related problems was 2.58%. In order to improve the availability of MRI equipment, the maintenance time of MRI equipment should be shortened.

Conclusions: RAM-based analysis of MRI equipment can help hospital equipment managers to carry out the work of operation optimization, maintenance strategy formulation and safety management of MRI equipment.
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http://dx.doi.org/10.3969/j.issn.1671-7104.2022.03.024DOI Listing
May 2022
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