Publications by authors named "Beatriz Juliao Aarestrup"

12 Publications

  • Page 1 of 1

Satisfaction of allergic patients treated with house dust mite sublingual immunotherapy.

Int J Immunopathol Pharmacol 2021 Jan-Dec;35:20587384211015528

Chairman Laboratory of Immunopathology and Experimental Pathology (CBR), Universidade Federal de Juiz de Fora, Chief Allergy and immunology, Faculdade de Ciências Médicas e da Saúde-SUPREMA e Hospital Maternidade Therezinha de Jesus, Juiz de Fora, Minas Gerais, Brasil.

Sublingual immunotherapy (SLIT) has been used for more than three decades as a therapeutic strategy for the treatment of allergic diseases. Studies have demonstrated its efficacy and safety, and numerous clinical trials have evaluated these parameters. In the present study, through patient perception, we investigated the patient satisfaction with the use of house dust mite SLIT treatment. "Satisfaction Scale for Patients Receiving Allergen Immunotherapy" (ESPIA) questionnaire, a standardized and validated instrument for clinical studies evaluating allergen immunotherapy, was applied to allergic patients ( = 136). Children and adults of both sexes who received SLIT for and/or , according to the results of an immediate reading puncture test, were included. Data analysis showed that the perception of treatment effectiveness was 92%, performance improvement in the daily activities was 91%, a satisfactory cost-benefit balance was 84%, and the perception of general satisfaction was 97%. The results showed a high perception of satisfaction in allergic patients undergoing house dust mite SLIT.
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http://dx.doi.org/10.1177/20587384211015528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127737PMC
November 2021

Induced oral mucositis in Wistar rats treated with different drugs: Preventive potential in cytokine production.

Mol Clin Oncol 2021 Jun 27;14(6):127. Epub 2021 Apr 27.

Laboratory of Immunopathology and Experimental Pathology, Reproductive Biology Center, Department of Morphology, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais 36036-330, Brazil.

The aim of the present study was to investigate the preventive potential of pentoxifylline, atorvastatin and trans-caryophyllene in oral mucositis through histopathological analysis of wounds in the oral mucosa of Wistar rats treated with 5-FU, and to evaluate the immunomodulatory effect of these drugs on serum nitrite production, IFN-γ, TNF-α and TGF-β, and TNF-α in tissues. A total of 32 male Wistar rats with an average age of 9 weeks and an average body weight of 250 g were divided into four treatment groups: Saline, trans-caryophyllene, pentoxifylline and atorvastatin. Oral mucositis was then induced. On days 3 and 4, the mucosa of the mouth of eight pre-treated animals in each group was bilaterally scarified twice with the tip of a sterile needle, with an anesthetic solution. Mucosal samples from animals treated with trans-caryophyllene preserved a thin epithelial lining associated with focal perivascular inflammatory infiltrates. Pentoxifylline-treated animals exhibited total epithelial loss in oral wounds with severe inflammatory infiltrates and mild re-epithelialization associated with mild and diffuse inflammatory infiltrates. Samples from atorvastatin-treated animals exhibited no epithelial dissolution, with preserved thin lining and mild diffuse inflammatory infiltrates. The analysis of TNF-α expression revealed improved results in trans-caryophyllene animals. The analysis of TGF-β expression revealed positive mononuclear cells. Preventive treatment with atorvastatin was demonstrated to modulate the serum expression levels of TNF-α during all stages of the experiment. Treatment with trans-caryophyllene modulated serum IFN-γ levels negatively, whereas treatment with atorvastatin and trans-caryophyllene maintained lower levels of IFN-γ compared with the control group.
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http://dx.doi.org/10.3892/mco.2021.2289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108041PMC
June 2021

Brazilian experience with atopy patch tests for , and .

World Allergy Organ J 2018 24;11(1):27. Epub 2018 Oct 24.

1Federal University of Juiz de Fora, Juiz de Fora, Brazil.

Background: The aim of this study was to evaluate the positivity rates of atopy patch tests for , and in patients with respiratory diseases such as asthma and allergic rhinitis with or without atopic dermatitis.

Methods: The patients' clinical histories were collected, and the patients were subjected to skin prick and patch tests with the three different house dust mites on the same day. The patch tests were examined 48 hours later, and then patients were divided into two groups: I- patients with respiratory diseases, such as asthma and/or rhinitis, and atopic dermatitis and II-patients with only respiratory diseases. A total of 74 patients ranging in age from 2 to 60 years were included in this study; 16 patients were included in group I and 58 were included in group II. This study was approved by the human ethics committee of the Faculty of Medical Science and Health SUPREMA (number 2.007.135), and written informed consent was collected from each patient or their parents prior to enrollment.

Results: In the skin prick tests, the most prevalent mite that evoked a reaction was , followed by and . Regarding the atopy patch tests, the mite that most frequently induced a positive reaction was (78.4%), followed by (77%) and (52.7%). A comparison of the skin prick and atopy patch tests revealed that 53 patients (71.6%) were positive on both tests, and 30 (56.6%) patients were positivite for the same mite. We found six patients (8%) who had a positive clinical history of allergy and only exhibited positivity on the atopy patch test.

Discussion: Most studies have been performed with atopic dermatitis patients, but in this study, most of the patients had respiratory conditions. is a mite that is prevalent in tropical areas, such as Brazil, and only two publications include these three mites, wich are present in Brazil. The APT may produce positive results in concordance with the SPT resuts, but may also be the only positive test ( 8%) as we observed in our study. These results suggest that the mite atopy patch test is relevant and should be considered as an additional test for patients with clinical histories of allergic respiratory disease who have negative prick test results.

Conclusion: The APT should be considered as an additional test when the SPT and specific serum IgE tests are negative in patients with clinical histories of allergies.
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http://dx.doi.org/10.1186/s40413-018-0206-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6199810PMC
October 2018

Fraction of exhaled nitric oxide measurements in the diagnoses of asthma in elderly patients.

Clin Interv Aging 2016 12;11:623-9. Epub 2016 May 12.

School of Medical and Health Sciences - SUPREMA, Federal University of Juiz de Fora, Institute of Biological Sciences, Juiz de Fora, Brazil; Maternity Hospital Terezinha de Jesus, Federal University of Juiz de Fora, Institute of Biological Sciences, Juiz de Fora, Brazil; Laboratory of Immunopathology and Experimental Pathology, Federal University of Juiz de Fora, Reproductive Biology Center (CBR), Juiz de Fora, Brazil.

Objective: To assess the value of fraction of exhaled nitric oxide (FeNO) measurements in the diagnosis of asthma in elderly patients.

Methods: The clinical symptoms of 202 elderly patients were assessed with the asthma module of the International Study of Asthma and Allergies in Childhood test, which had been modified for the elderly patients, and the diagnostic routine for chronic obstructive pulmonary disease (COPD), which was based on the Global initiative for chronic Obstructive Lung Disease criteria. Of the 202 patients assessed, 43 were subjected to pulmonary function evaluations (spirometry) and FeNO measurements.

Results: Of the 202 elderly patients, 34 had asthma (23 definite and eleven probable), 20 met COPD criteria, 13 presented with an overlap of asthma and COPD, and 135 did not fit the criteria for obstructive pulmonary disease. Among the 43 elderly patients who were subjected to FeNO measurements, ten showed altered results (23.2%) and 33 had normal results (76.7%). The average value of FeNO in patients with definite and probable asthma undergoing this procedure was 29.2 parts per billion whereas that in nonasthmatic patients was 17.5 parts per billion (P=0.0002).

Conclusion: We show a clear relationship between FeNO levels and asthma symptoms and previous asthma diagnoses in elderly patients.
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http://dx.doi.org/10.2147/CIA.S94741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4869629PMC
February 2017

Atorvastatin and trans-caryophyllene for the prevention of leukopenia in an experimental chemotherapy model in Wistar rats.

Mol Clin Oncol 2015 Jul 9;3(4):825-828. Epub 2015 Apr 9.

Laboratory of Immunopathology and Clinical Immunology, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil ; Department of Morphology, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.

Malignant neoplasia represents the second cause of disease-related mortality and, among all patients diagnosed with cancer, 70% will receive chemotherapy during the course of treatment. As a consequence, an increasing number of researchers have focused their attention on the search for more specific anticancer therapies associated with fewer side effects. Leukopenia is an important adverse effect associated with chemotherapy. Secondary infection is very common among leukopenic patients, directly affecting the continuity of the chemotherapeutic treatment and leading to possible complications in tumor immune defense. Atorvastatin, a type of statin, is a known agent used to control hypercholesterolemia. Trans-caryophyllene, isolated from a resinous oil extracted from the copaiba tree, possesses anti-inflammatory and analgesic properties. The AIM of the present study was to evaluate, through a complete leukocyte count, the systemic immunomodulation potential of pentoxifylline (PTX), atorvastatin and trans-caryophyllene, as well as the possible prophylactic role of these drugs against secondary leukopenia, in an experimental chemotherapy model induced by 5-fluorouracil (5-FU) in wistar rats. A total of 32 male wistar rats were used, 24 of which were submitted to treatment with atorvastatin, PTX and trans-caryophyllene prior to the administration of chemotherapy. The Shapiro-Wilk test was used to verify normality and the Kruskal-Wallis test was used for negative data in the normality test. Among the drugs selected, atorvastatin exhibited the best preventive potential in regards to leukopenia secondary to experimental chemotherapy induced by 5-FU, in comparison to the group receiving saline solution, while PTX amplified such alterations in the leukograms of the animals in this trial.
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http://dx.doi.org/10.3892/mco.2015.544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4487044PMC
July 2015

Oral mucositis in cancer treatment: Natural history, prevention and treatment.

Mol Clin Oncol 2014 May 7;2(3):337-340. Epub 2014 Feb 7.

Department of Morphology (Histology and Embryology), Institute of Biological Sciences Research, Federal University of Juiz de Fora, Minas Gerais, Brazil.

Oral mucositis is a condition that is characterized by ulcerative lesions in the mucosa of patients undergoing radiotherapy or chemotherapy. Oral mucositis is currently considered to be the most severe complication of anticancer therapy, affecting 40-80% of patients undergoing chemotherapy and almost all those undergoing radiotherapy of the head and neck. Although they do not prevent lesions from appearing, drugs for the treatment of oral mucositis are required to minimize its clinical aggressiveness and improve the nutritional status, hydration and quality of life of the affected patients. Furthermore, the prevention and control of oral ulcers is crucial for cancer prognosis, since the establishment of severe lesions may lead to temporary or permanent treatment discontinuation and compromise cancer control. The objective of this study was to present a review on this condition, its causes and its treatment to professional clinical dentists, in order to help minimize patient suffering. A search was conducted through PubMed, Lilacs and MedLine, to retrieve related articles published between 1994 and 2013.
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http://dx.doi.org/10.3892/mco.2014.253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999143PMC
May 2014

Body weight gain and serum leptin levels of non-overweight and overweight/obese pregnant women.

Med Sci Monit 2013 Nov 22;19:1043-9. Epub 2013 Nov 22.

Background: Our objective was to evaluate changes in serum leptin levels during pregnancy in overweight/obese and non-obese women and to assess total and percent weight gain during pregnancy as possible factors that influence leptin levels.

Material And Methods: In a prospective study of 42 low-risk pregnant women receiving prenatal care, we assessed serum leptin levels at gestational weeks 9-12, 25-28, and 34-37. Based on their pre-pregnancy body mass indices (BMIs), the cohort was divided into: non-overweight (BMI <25 kg/m(2)) and overweight/obese (BMI ≥ 25 kg/m(2)) subjects.

Results: We found a progressive increase in maternal weight gain during pregnancy in both groups. There was also a progressive increase in leptin levels in the 2 strata; however, the increase was significantly higher in the non-overweight patient group. We found that non-overweight pregnant women had a noticeably larger total weight gain. When analyzing the percent weight gain during pregnancy compared to the pre-pregnancy weight, the non-overweight group had a significantly greater percent weight gain than the overweight/obese group.

Conclusions: Our results suggest that the greater increase in leptin levels in non-overweight pregnant women can be explained by the higher percent weight gain in this group compared to overweight/obese women. These findings suggest that controlling the percent weight gain may be an important preventive measure when controlling leptin levels during pregnancy and subsequent medical complications.
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http://dx.doi.org/10.12659/MSM.884027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3853999PMC
November 2013

Clinical protocol for punctuated 88% phenol peels in the treatment of photoaging: a histopathological study of three cases.

Dermatol Surg 2012 Dec 28;38(12):2011-5. Epub 2012 Aug 28.

Laboratory of Immunopathology and Experimental Pathology/CBR, Federal University of Juiz de Fora, Juiz de Fora, Brazil.

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http://dx.doi.org/10.1111/j.1524-4725.2012.02543.xDOI Listing
December 2012

[Waist circumference measured before the 12th week of pregnancy: correlation with serum leptin levels].

Rev Bras Ginecol Obstet 2012 Jun;34(6):268-73

Disciplina de Obstetrícia do Curso de Medicina da Faculdade de Ciências Médicas e da Saúde de Juiz de Fora - Suprema - Juiz de Fora (MG), Brasil.

Purpose: To evaluate the correlation between maternal waist circumference measured before the 12th week of gestation and serum leptin levels during pregnancy, as well as to compare the leptin levels of women with and without abdominal obesity diagnosed in early pregnancy.

Methods: Prospective study including 40 pregnant women receiving low-risk prenatal care, older than 20 years, nonsmokers, with singleton pregnancies and without chronic disease. Waist circumference was measured before the 12th week and serum leptin levels were measured between the 9th and 12th, 25th and 28th and 34th and 37th weeks of gestation. According to waist circumference measurement, the cohort was divided into two groups: with and without abdominal obesity. The Mann-Whitney and χ(2) tests were used to assess the differences between groups. The Pearson correlation coeffient was used to assess the association between waist circumference and serum leptin levels during pregnancy. The level of significance was set at p<0.05.

Results: The mean weight and body mass index of patients with abdominal obesity (74.4±11.0 kg/28.9±4.1) was higher than that of patients without abdominal obesity (55.6±5.9 kg/21.1±2.4) (p=0.001). The mean leptin levels in pregnant patients with abdominal obesity (41.9±3.5 ng/mL) was higher than in patients without abdominal obesity (23.6±2.7 ng/mL) (p<0.0002). A positive correlation was obtained between the waist circumference measured during the same period and the mean serum leptin levels (r=0.7; p<0.0001).

Conclusions: Waist circumference measured before the 12th week of pregnancy is a valid and simple method to predict the serum leptin levels throughout pregnancy. Pregnant women with abdominal obesity diagnosed before 12th week have higher mean serum leptin levels during pregnancy than those without abdominal obesity.
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June 2012

The effect of the Ginkgo biloba extract in the expression of Bax, Bcl-2 and bone mineral content of Wistar rats with glucocorticoid-induced osteoporosis.

Phytother Res 2013 Apr 31;27(4):515-20. Epub 2012 May 31.

Centro de Biologia da Reprodução-Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.

Objective: Evaluate the effects of the extract of Ginkgo biloba (EGb) in the glucocorticoid-induced-osteoporosis through the Bax and Bcl-2 expressions by osteoblast cells, the x-ray and bone density of the tibia.

Method: Rats were divided into five groups: osteoporosis; EGb1 (28 mg/kg); EGb2 (56 mg/kg); alendronate (0.2 mg/animal) and control. The treatments were conducted for 20 (n = 30) and 30 days (n = 30). The Bax and Bcl-2 expressions were evaluated in osteoblasts of the mandibular alveolar bone. The tibias were radiographed to evaluate the X-ray and bone density. The control group was compared with the osteoporosis' (Student's t-test/Mann-Whitney). The other groups were analyzed by analysis of variance test followed by Dunnett/Dunnett T3 (p < 0.05).

Results: When compared the osteoporosis to the control group (p <0.05): Bax and x-ray density increased; Bcl-2 and the bone density reduced. When compared with the osteoporosis group (p < 0.05), alendronate (30 days), EGb1 and EGb2 (20/30 days) increased the Bcl-2 expression; EGb2 and alendronate (20 days) EGb1 and EGb2 (30 days) reduced the Bax expression; and EGb1 and EGb2 (20/30 days) reduced the X-ray density.

Conclusions: The EGb improved the Bcl-2 and reduced the Bax expression by osteoblasts in the mandibular alveolar bone and recovered the mineral content in the tibia of rats with glucocorticoid-induced-osteoporosis.
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http://dx.doi.org/10.1002/ptr.4747DOI Listing
April 2013

Effects of aqueous extract of Echinodorus grandiflorus on the immune response in ovalbumin-induced pulmonary allergy.

Ann Allergy Asthma Immunol 2011 Jun 10;106(6):481-8. Epub 2011 Feb 10.

Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Minas Gerais, Brazil.

Background: Asthma is a disease characterized by intermittent obstruction of the airways and chronic inflammation that affects approximately 300 million people worldwide. The immune response in asthma is predominantly T(H)2, with high levels of total and allergen-specific IgE and bronchial eosinophilia. Asthma treatment is aimed at controlling the disease, and the drugs used currently have systemic adverse effects and generally are not effective in difficult-to-control cases.

Objective: To investigate the effect of aqueous extract of Echinodorus grandiflorus, a plant used in folk medicine for its diuretic and anti-inflammatory properties, in a model of pulmonary allergy.

Methods: BALB/c mice were intraperitoneally sensitized and nasally challenged with ovalbumin. Aqueous extract and dexamethasone treatments (0.1 mL/d per mouse) were initiated on day 32 and concluded on day 40. Eight hours after the last challenge evaluations, of serum, bronchoalveolar lavage, and lung tissue were performed.

Results: Oral treatment with the extract markedly reduced the number of total cells and eosinophils in bronchoalveolar lavage. The eosinophil peroxidase activity in lung tissue, the levels of ovalbumin-specific IgE in serum, the levels of CCL11, and the gene expression of interleukin 4 and interleukin 13 in lung tissue were also lower after treatment.

Conclusions: These results suggest that the aqueous extract of E grandiflorus is able to modulate allergic pulmonary inflammation and may be useful as a potential therapeutic agent for asthma.
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http://dx.doi.org/10.1016/j.anai.2011.01.008DOI Listing
June 2011

Effect of thalidomide and pentoxifylline on experimental autoimmune encephalomyelitis (EAE).

Exp Neurol 2010 Nov 18;226(1):15-23. Epub 2010 Apr 18.

Fluminense Federal University, FCB, Nova Friburgo, Rio de Janeiro, Brazil.

Background: Autoimmune encephalomyelitis (EAE) in Lewis rats is a classical experimental model of demyelinating inflammatory disease of the central nervous system. EAE is widely accepted for study of immune-inflammatory mechanisms in the CNS related to multiple sclerosis (MS) due to similar clinical evolution.

Objectives: In the present study we investigated the effects of Thalidomide and pentoxifylline during EAE development in Lewis rats.

Methods: EAE was induced in Lewis rats and treatment with Thalidomide or pentoxifylline was performed. Clinical evaluation was carried out daily. Histopathological analysis of the brain tissue and spinal cord was performed. Griess method was used for determination of NO serum levels. TNF-alpha and IFN-gamma serum levels were investigated using ELISA method.

Results: Thalidomide and pentoxifylline treatment is associated with significant reduction of neuroinflammation in CNS. Serum levels of NO, IFN-gamma and TNF-alpha showed a marked reduction. Such findings were correlated with improvement of clinical symptoms, particularly in thalidomide treated rats.

Conclusions: Taken together the data suggested that thalidomide and pentoxifylline may be therapeutic options for the treatment of MS, however further experiments must be performed to investigate this hypothesis.
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http://dx.doi.org/10.1016/j.expneurol.2010.04.007DOI Listing
November 2010
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