4,023 results match your criteria zipper domain

The lipid-binding START domain regulates the dimerization of ATML1 via modulating the ZIP motif activity in Arabidopsis thaliana.

Dev Growth Differ 2021 Sep 20. Epub 2021 Sep 20.

Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1, Komaba, Meguro-ku, 153-8902, Japan.

In Arabidopsis thaliana, the epidermis is the outermost cell layer composed of many specialized types of epidermal cells, such as pavement cells, trichomes, and guard cells. The homeodomain-leucine zipper (HD-ZIP) class Ⅳ transcription factors (TFs), which are unique to the plant kingdom, have been recognized as key regulators of epidermis development. Unlike animal HD proteins, which can bind to DNA as monomers, plant HD-ZIP class Ⅳ TFs bind to DNA as dimers, although little is known about the regulation of their dimerization process. Read More

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September 2021

Novel anti-repression mechanism of H-NS proteins by a phage protein.

Nucleic Acids Res 2021 Sep 14. Epub 2021 Sep 14.

Department of Macromolecular Biochemistry, Leiden Institute of Chemistry, Einsteinweg 55, 2333 CC Leiden, The Netherlands.

H-NS family proteins, bacterial xenogeneic silencers, play central roles in genome organization and in the regulation of foreign genes. It is thought that gene repression is directly dependent on the DNA binding modes of H-NS family proteins. These proteins form lateral protofilaments along DNA. Read More

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September 2021

Genome-wide prediction of cauliflower miRNAs and lncRNAs and their roles in post-transcriptional gene regulation.

Planta 2021 Sep 14;254(4):72. Epub 2021 Sep 14.

Department of Biotechnology, Visva-Bharati University, Santiniketan, 731235, India.

Main Conclusion: We have predicted miRNAs, their targets and lncRNAs from the genome of Brassica oleracea along with their functional annotation. Selected miRNAs and their targets are experimentally validated. Roles of these non-coding RNAs in post-transcriptional gene regulation are also deciphered. Read More

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September 2021

TaFDL2-1A interacts with TabZIP8-7A protein to cope with drought stress via the abscisic acid signaling pathway.

Plant Sci 2021 Oct 14;311:111022. Epub 2021 Aug 14.

State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address:

Drought has negative effects on cereal production. Studies have shown that many basic leucine zipper transcription factors (bZIP TFs) help to cope with drought stress. In this study, bZIP TF wheat (Triticum aestivum L. Read More

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October 2021

An ionic lock and a hydrophobic zipper mediate the coupling between an insect pheromone receptor BmOR3 and downstream effectors.

J Biol Chem 2021 Sep 1:101160. Epub 2021 Sep 1.

Key Laboratory Experimental Teratology of the Ministry of Education and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shandong University, 250012 Jinan, China; Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, 100191 Beijing, China. Electronic address:

Pheromone receptors (PRs) recognize specific pheromone compounds to guide the behavioral outputs of insects, which are the most diverse group of animals on earth. The activation of PRs is known to couple to the calcium permeability of their coreceptor (Orco) or putatively with G proteins; however, the underlying mechanisms of this process are not yet fully understood. Moreover, whether this transverse seven transmembrane domain (7TM)-containing receptor is able to couple to arrestin, a common effector for many conventional 7TM receptors, is unknown. Read More

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September 2021

Comparative analysis of the MyTH4-FERM myosins reveals insights into the determinants of actin track selection in polarized epithelia.

Mol Biol Cell 2021 Sep 2:mbcE20070494. Epub 2021 Sep 2.

Department of Biological Sciences, University of Toledo, Toledo, Ohio 43606.

MyTH4-FERM (MF) myosins evolved to play a role in the creation and function of a variety of actin-based membrane protrusions that extend from cells. Here, we performed an analysis of the MF myosins, Myo7A, Myo7B, and Myo10, to gain insight into how they select for their preferred actin networks. Using enterocytes that create spatially separated actin tracks in the form of apical microvilli and basal filopodia, we show that actin track selection is principally guided by the mode of oligomerization of the myosin along with the identity of the motor domain, with little influence from the specific composition of the lever arm. Read More

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September 2021

Prognostic impact of CEBPA bZIP domain mutation in acute myeloid leukemia.

Blood Adv 2021 Aug 27. Epub 2021 Aug 27.

National Cancer Center Hospital, Tokyo.

Mutations of CCAAT/enhancer binding protein alpha (CEBPAmu) are found in 10-15% of de novo acute myeloid leukemia (AML) cases. Double-mutated CEBPA (CEBPAdm) is associated with a favorable prognosis; however, single-mutated CEBPA (CEBPAsm) does not appear to improve prognosis. We investigated the CEBPAmu for prognosis in 1028 AML patients, registered in the Multi-center Collaborative Program for Gene Sequencing of Japanese AML. Read More

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HD-ZIP Gene Family: Potential Roles in Improving Plant Growth and Regulating Stress-Responsive Mechanisms in Plants.

Genes (Basel) 2021 Aug 17;12(8). Epub 2021 Aug 17.

Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.

Exploring the molecular foundation of the gene-regulatory systems underlying agronomic parameters or/and plant responses to both abiotic and biotic stresses is crucial for crop improvement. Thus, transcription factors, which alone or in combination directly regulated the targeted gene expression levels, are appropriate players for enlightening agronomic parameters through genetic engineering. In this regard, homeodomain leucine zipper (HD-ZIP) genes family concerned with enlightening plant growth and tolerance to environmental stresses are considered key players for crop improvement. Read More

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Nucleobindin-2 consists of two structural components: The Zn-sensitive N-terminal half, consisting of nesfatin-1 and -2, and the Ca-sensitive C-terminal half, consisting of nesfatin-3.

Comput Struct Biotechnol J 2021 30;19:4300-4318. Epub 2021 Jul 30.

Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.

Nucleobindin-2 (Nucb2) is a protein that has been suggested to play roles in a variety of biological processes. Nucb2 contains two Ca/Mg-binding EF-hand domains separated by an acidic amino acid residue-rich region and a leucine zipper. All of these domains are located within the C-terminal half of the protein. Read More

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Insights into the Mechanistic Perspective Effect of Insulin on the Nonamyloidogenic Component (NAC) and α-Synuclein Aggregation.

ACS Chem Neurosci 2021 09 18;12(17):3266-3276. Epub 2021 Aug 18.

Department of Chemistry, Ben-Gurion University of the Negev, Be'er Sheva84105, Israel.

Insulin plays important functions in the brain, such as neuroprotective effects on neurons, and it is also involved in cognitive functions (e.g., attention, learning and memory). Read More

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September 2021

Adiponectin inhibits D‑gal‑induced cardiomyocyte senescence via AdipoR1/APPL1.

Mol Med Rep 2021 Oct 13;24(4). Epub 2021 Aug 13.

Department of Geriatrics, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, P.R. China.

The aim of the present study was to examine whether adiponectin could inhibit cardiomyocyte senescence induced by D‑galactose (D‑gal), and whether it functioned via the adiponectin receptor 1 (AdipoR1)/adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1) signaling pathway. For this purpose, the expression levels of adiponectin, AdipoR1 and APPL1 in mouse plasma and myocardial tissues were detected via reverse transcription‑quantitative PCR (RT‑qPCR) and western blotting. An adiponectin‑overexpression plasmid was transfected into D‑gal‑treated H9c2 cells prior to the detection of AdipoR1 and APPL1 expression by RT‑qPCR. Read More

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October 2021

Recombinant antibodies recognize conformation-dependent epitopes of the leucine zipper of misfolding-prone myocilin.

J Biol Chem 2021 Aug 9;297(3):101067. Epub 2021 Aug 9.

School of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA. Electronic address:

Recombinant antibodies with well-characterized epitopes and known conformational specificities are critical reagents to support robust interpretation and reproducibility of immunoassays across biomedical research. For myocilin, a protein prone to misfolding that is associated with glaucoma and an emerging player in other human diseases, currently available antibodies are unable to differentiate among the numerous disease-associated protein states. This fundamentally constrains efforts to understand the connection between myocilin structure, function, and disease. Read More

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Structural Diversities of Lectins Binding to the Glycosphingolipid Gb3.

Front Mol Biosci 2021 26;8:704685. Epub 2021 Jul 26.

Faculty of Biology, University of Freiburg, Freiburg, Germany.

Glycolipids are present on the surfaces of all living cells and thereby represent targets for many protein receptors, such as lectins. Understanding the interactions between lectins and glycolipids is essential for investigating the functions of lectins and the dynamics of glycolipids in living membranes. This review focuses on lectins binding to the glycosphingolipid globotriaosylceramide (Gb3), an attractive host cell receptor, particularly for pathogens and pathogenic products. Read More

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Siberian Wildrye ( L.) Abscisic Acid-Insensitive 5 Gene Is Involved in Abscisic Acid-Dependent Salt Response.

Plants (Basel) 2021 Jul 2;10(7). Epub 2021 Jul 2.

Chinese Academy of Agricultural Sciences, Grassland Research Institute, Hohhot 010010, China.

Siberian wildrye ( L.) is a salt-tolerant, high-quality forage grass that plays an important role in forage production and ecological restoration. Abscisic acid (ABA)-insensitive 5 () is essential for the normal functioning of the ABA signal pathway. Read More

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DAXX ameliorates metabolic dysfunction in mice with diet-induced obesity by activating the AMP-activated protein kinase-related kinase MPK38/MELK.

Biochem Biophys Res Commun 2021 Oct 3;572:164-170. Epub 2021 Aug 3.

Department of Biochemistry, School of Biological Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea. Electronic address:

Death domain-associated protein (DAXX) is involved in the activation of adipocyte apoptosis and is downregulated in response to a high-fat diet (HFD), which implies that the inhibition of adipocyte apoptosis may cause obesity. However, the anti-obesity effects of DAXX in diet-induced obesity (DIO) remain to be characterized. Here, we identified DAXX as an interacting partner of murine protein serine-threonine kinase 38 (MPK38). Read More

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October 2021

A Quantitative IRE1a Ribonuclease-Dependent mRNA Cleavage Assay for Functional Studies of Substrate Splicing and Decay Activities.

Front Plant Sci 2021 20;12:707378. Epub 2021 Jul 20.

Department of Biology, The University of Alabama at Birmingham, Birmingham, AL, United States.

The unfolded protein response (UPR) is an adaptive eukaryotic reaction that controls the protein folding capacities of the endoplasmic reticulum (ER). The most ancient and well-conserved component of the UPR is Inositol-Requiring Enzyme 1 (IRE1). Arabidopsis IRE1a (AtIRE1) is a transmembrane sensor of ER stress equipped with dual protein kinase and ribonuclease (RNase) activities, encoded by its C-terminal domain. Read More

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BZW2/5MP1 acts as a promising target in hepatocellular carcinoma.

J Cancer 2021 22;12(17):5125-5135. Epub 2021 Jun 22.

Department of Translational Medicine Center, Affiliated Hospital of Hangzhou Normal University, Institute of Hepatology and Metabolic Diseases of Hangzhou Normal University, Hangzhou, Zhejiang 310015, P.R. China.

Basic leucine zipper and W2 domain 2 (BZW2), also known as 5MP1, is a protein related to translation regulation. Evidence from previous studies indicates that BZW2 is involved in tumorigenesis in several cancers. However, little is known about the role of BZW2 in hepatocellular carcinoma (HCC). Read More

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Coiled-coil binding of the leucine zipper domains of APOL1 is necessary for the open cation channel conformation.

J Biol Chem 2021 Jul 28:101009. Epub 2021 Jul 28.

Department of Biological sciences, Hunter College, City University of New York, USA. Electronic address:

Apolipoprotein L-I (APOL1) is a channel-forming component necessary for innate immunity. The common human APOL1 variant G0 provides protection against infection with certain Trypanosoma and Leishmania parasite species, but it cannot protect against the trypanosomes responsible for human African trypanosomiasis (HAT). Human APOL1 variants G1 and G2 protect against human-infective trypanosomes, but also confer a higher risk of developing chronic kidney disease. Read More

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PTB domain and leucine zipper motif 1 (APPL1) inhibits myocardial ischemia/hypoxia-reperfusion injury via inactivation of apoptotic protease activating factor-1 (APAF-1)/Caspase9 signaling pathway.

Bioengineered 2021 12;12(1):4385-4396

Center of Geriatrics, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou, PR China.

Myocardial ischemia/hypoxia-reperfusion injury mediates the progression of multiple cardiovascular diseases. It has been reported that knockdown of adaptor protein containing a PH domain, PTB domain and leucine zipper motif 1 (APPL1) is a significant factor for the progression of myocardial injury. However, the role of APPL1 in myocardial ischemia remains unclear. Read More

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December 2021

The impact of bZIP Atf1ortholog global regulators in fungi.

Appl Microbiol Biotechnol 2021 Aug 24;105(14-15):5769-5783. Epub 2021 Jul 24.

Department of Molecular Biotechnology and Microbiology, Institute of Biotechnology, University of Debrecen, P.O. Box 63, Debrecen, H-4010, Hungary.

Regulation of signal transduction pathways is crucial for the maintenance of cellular homeostasis and organismal development in fungi. Transcription factors are key elements of this regulatory network. The basic-region leucine zipper (bZIP) domain of the bZIP-type transcription factors is responsible for DNA binding while their leucine zipper structural motifs are suitable for dimerization with each other facilitiating the formation of homodimeric or heterodimeric bZIP proteins. Read More

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BATF regulates progenitor to cytolytic effector CD8 T cell transition during chronic viral infection.

Nat Immunol 2021 08 19;22(8):996-1007. Epub 2021 Jul 19.

Blood Research Institute, Versiti, Milwaukee, WI, USA.

During chronic viral infection, CD8 T cells develop into three major phenotypically and functionally distinct subsets: Ly108TCF-1 progenitors, Ly108CXCR1 terminally exhausted cells and the recently identified CXCR1 cytotoxic effector cells. Nevertheless, how CXCR1 effector cell differentiation is transcriptionally and epigenetically regulated remains elusive. Here, we identify distinct gene regulatory networks and epigenetic landscapes underpinning the formation of these subsets. Read More

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Monitoring Human Neutrophil Activation by a Proteinase 3 Near-Infrared Fluorescence Substrate-Based Probe.

Bioconjug Chem 2021 08 16;32(8):1782-1790. Epub 2021 Jul 16.

Université de Tours, Tours 37032, France.

A near-infrared fluorescent (NIRF) substrate-based probe (SBP) was conceived to monitor secreted human proteinase 3 (hPR3) activity. This probe, called pro3-SBP, is shaped by a fused peptide hairpin loop structure, which associates a hPR3 recognition domain (Val-Ala-Asp-Nva-Ala-Asp-Tyr-Gln, where Nva is norvaline) and an electrostatic zipper (consisting of complementary polyanionic (d-Glu) and polycationic (d-Arg) sequences) in close vicinity of the N- and C-terminal FRET couple (fluorescent donor, sulfoCy5.5; dark quencher, QSY21). Read More

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lncRNA SNHG6 promotes hepatocellular carcinoma progression by interacting with HNRNPL/PTBP1 to facilitate SETD7/LZTFL1 mRNA destabilization.

Cancer Lett 2021 Nov 10;520:121-131. Epub 2021 Jul 10.

Department of Hepatobiliary and Pancreas, Research Center of Digestive Diseases, Zhongnan Hospital, Wuhan University, Wuhan, Hubei, China. Electronic address:

The lncRNA SNHG6 (small nucleolar RNA host gene 6) plays vital roles in tumorigenesis and the progression of hepatocellular carcinoma (HCC). However, the regulatory mechanisms of SNHG6 are largely unknown. In this study, we identified, via quantitative proteomics, specific cytoskeleton-associated proteins and enzyme modulators to be potential targets of SNHG6. Read More

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November 2021

Structural basis for the dimerization mechanism of human transcription factor E3.

Biochem Biophys Res Commun 2021 Sep 2;569:41-46. Epub 2021 Jul 2.

State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 200032, China; School of Chemistry and Materials Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, 1 Sub-lane Xiangshan, Hangzhou, 310024, China. Electronic address:

The transcription factor for immunoglobulin heavy-chain enhancer 3 (TFE3) is a member of the microphthalmia (MiT/TFE) transcription factor family. Dysregulation of TFE3 due to chromosomal abnormalities is associated with a subset of human renal cell carcinoma. Little structural information of this key transcription factor has been reported. Read More

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September 2021

Generation of a Mouse Model to Study the Noonan Syndrome Gene in the Telencephalon.

Front Cell Dev Biol 2021 16;9:673995. Epub 2021 Jun 16.

Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States.

The leucine zipper-like transcriptional regulator 1 () is a BTB-Kelch domain protein involved in RAS/MAPK pathway regulation. Mutations in are associated with cancers and Noonan syndrome, the most common RASopathy. The expression and function of in the developing brain remains poorly understood. Read More

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MicroRNA-30c-5p protects against myocardial ischemia/reperfusion injury via regulation of Bach1/Nrf2.

Toxicol Appl Pharmacol 2021 09 2;426:115637. Epub 2021 Jul 2.

Department of Cardiology, The First Hospital of Shanxi Medical University, No. 85 Jiefang South Road, Taiyuan 030001, China. Electronic address:

MicroRNAs (miRNAs) are critical regulatory factors in myocardial ischemia/reperfusion (I/R) injury. The miRNA miR-30c-5p has been reported as a key mediator in several myocardial abnormalities. However, the precise roles and mechanisms of miR-30c-5p in myocardial I/R injury remain not well-studied. Read More

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September 2021

MaAreB, a GATA Transcription Factor, Is Involved in Nitrogen Source Utilization, Stress Tolerances and Virulence in .

J Fungi (Basel) 2021 Jun 27;7(7). Epub 2021 Jun 27.

Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing 401331, China.

The nitrogen catabolite repression (NCR) pathway is involved in nitrogen utilization, in which the global GATA transcription factor AreA plays an indispensable role and has been reported in many fungi. However, relatively few studies are focused on AreB, another GATA transcription factor in the NCR pathway and the functions of AreB are largely unknown in entomopathogenic fungi. Here, we characterized MaAreB in the model entomopathogenic fungus . Read More

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Circ_0081001 down-regulates miR-494-3p to enhance BACH1 expression and promotes osteosarcoma progression.

Aging (Albany NY) 2021 06 30;13(13):17274-17284. Epub 2021 Jun 30.

Department of Orthopedics, Shaanxi Provincial People's Hospital, Xian 710068, Shanxi, China.

The study was aimed at deciphering the function and mechanism of circ_0081001 in osteosarcoma (OS). In this study, quantitative real-time polymerase chain reaction (qRT-PCR) was utilized for quantifying circ_0081001, miR-494-3p, and BTB domain and CNC homolog 1 (BACH1) mRNA expressions in OS tissues and cells. Cell counting kit-8 (CCK-8) assay, together with 5-Ethynyl-2'-deoxyuridine (EdU) assay, was performed for evaluating cell proliferation; the alterations in apoptosis were analyzed utilizing flow cytometry; Transwell assay was conducted for examining cell migration and invasion; moreover, Western blot was utilized for the quantification of BACH1 protein expression; bioinformatics, dual-luciferase reporter gene, and RNA-binding protein immunoprecipitation assays were executed for validating the binding relationships between circ_0081001 and miR-494-3p, and between miR-494-3p and BACH1. Read More

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Cryptic association of B7-2 molecules and its implication for clustering.

Protein Sci 2021 Sep 10;30(9):1958-1973. Epub 2021 Jul 10.

Biological Sciences Division, Poornaprajna Institute of Scientific Research (PPISR), Bengaluru, India.

T-cell co-stimulation through CD28/CTLA4:B7-1/B7-2 axis is one of the extensively studied pathways that resulted in the discovery of several FDA-approved drugs for autoimmunity and cancer. However, many aspects of the signaling mechanism remain elusive, including oligomeric association and clustering of B7-2 on the cell surface. Here, we describe the structure of the IgV domain of B7-2 and its cryptic association into 1D arrays that appear to represent the pre-signaling state of B7-2 on the cell membrane. Read More

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September 2021

Dynein light chain-dependent dimerization of Egalitarian is essential for maintaining oocyte fate in Drosophila.

Dev Biol 2021 Oct 25;478:76-88. Epub 2021 Jun 25.

Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1460 Laney Walker Blvd, Augusta, GA, 30912, USA. Electronic address:

Egalitarian (Egl) is an RNA adaptor for the Dynein motor and is thought to link numerous, perhaps hundreds, of mRNAs with Dynein. Dynein, in turn, is responsible for the transport and localization of these mRNAs. Studies have shown that efficient mRNA binding by Egl requires the protein to dimerize. Read More

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October 2021