8,406 results match your criteria zikv

A vaccine inducing solely cytotoxic T lymphocytes fully prevents Zika virus infection and fetal damage.

Cell Rep 2021 May;35(6):109107

Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA. Electronic address:

As vaccine-induced non-neutralizing antibodies may cause antibody-dependent enhancement of Zika virus (ZIKV) infection, we test a vaccine that induces only specific cytotoxic T lymphocytes (CTLs) without specific antibodies. We construct a DNA vaccine expressing a ubiquitinated and rearranged ZIKV non-structural protein 3 (NS3). The protein is immediately degraded and processed in the proteasome for presentation via major histocompatibility complex (MHC) class I for CTL generation. Read More

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STAT2-dependent restriction of Zika virus by human macrophages but not dendritic cells.

Emerg Microbes Infect 2021 May 12:1-42. Epub 2021 May 12.

State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.

Zika virus (ZIKV) is an emerging mosquito-borne flavivirus that poses significant threats to global public health. Macrophages and dendritic cells are both key sentinel cells in the host immune response and play critical roles in the pathogenesis of flavivirus infections. Recent studies showed that ZIKV could productively infect monocyte-derived dendritic cells (moDCs), but the role of macrophages in ZIKV infection remains incompletely understood. Read More

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Dengue virus-free defective interfering particles have potent and broad anti-dengue virus activity.

Commun Biol 2021 May 11;4(1):557. Epub 2021 May 11.

Department of Cell and Molecular Biology, QIMR Berghofer Medical Research Institute, Herston, QLD, Australia.

Dengue virus (DENV) is spread from human to human through the bite of the female Aedes aegypti mosquito and leads to about 100 million clinical infections yearly. Treatment options and vaccine availability for DENV are limited. Defective interfering particles (DIPs) are considered a promising antiviral approach but infectious virus contamination has limited their development. Read More

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Reduced competence to arboviruses following the sustainable invasion of Wolbachia into native Aedes aegypti from Southeastern Brazil.

Sci Rep 2021 May 11;11(1):10039. Epub 2021 May 11.

Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor, Instituto René Rachou, Fiocruz Minas, Belo Horizonte, MG, Brazil.

Field release of Wolbachia-infected Aedes aegypti has emerged as a promising solution to manage the transmission of dengue, Zika and chikungunya in endemic areas across the globe. Through an efficient self-dispersing mechanism, and the ability to induce virus-blocking properties, Wolbachia offers an unmatched potential to gradually modify wild Ae. aegypti populations turning them unsuitable disease vectors. Read More

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Lying in wait: the resurgence of dengue virus after the Zika epidemic in Brazil.

Nat Commun 2021 May 11;12(1):2619. Epub 2021 May 11.

Department of Epidemiology of Microbial Diseases, Yale School of Public Health, Yale University, New Haven, CT, USA.

After the Zika virus (ZIKV) epidemic in the Americas in 2016, both Zika and dengue incidence declined to record lows in many countries in 2017-2018, but in 2019 dengue resurged in Brazil, causing ~2.1 million cases. In this study we use epidemiological, climatological and genomic data to investigate dengue dynamics in recent years in Brazil. Read More

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Potent programmable antiviral against dengue virus in primary human cells by Cas13b RNP with short spacer and delivery by virus-like particle.

Mol Ther Methods Clin Dev 2021 May 1. Epub 2021 May 1.

National Center for Genetic Engineering and Biotechnology, Klong Luang, Pathumthani 12120.

With sequencing as a standard frontline protocol to identify emerging viruses such Zika virus and SARS-CoV-2, direct utilization of sequence data to program antivirals against the viruses could accelerate drug development to treat their infections. CRISPR-Cas effectors are promising candidates that could be programmed to inactivate viral genetic material based on sequence data but several challenges such as delivery and design of effective crRNA need to be addressed to realize practical use. Here, we showed that virus-like particle (VLP) could deliver PspCas13b-crRNA ribonucleoprotein (RNP) in nanomolar range to efficiently suppress dengue virus infection in primary human target cells. Read More

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Zika Virus Infection of Human Iris Pigment Epithelial Cells.

Front Immunol 2021 22;12:644153. Epub 2021 Apr 22.

Precision Medicine Theme, South Australian Health & Medical Research Institute, Adelaide, SA, Australia.

During recent Zika epidemics, adults infected with Zika virus (ZIKV) have developed organ-specific inflammatory complications. The most serious Zika-associated inflammatory eye disease is uveitis, which is commonly anterior in type, affecting both eyes and responding to corticosteroid eye drops. Mechanisms of Zika-associated anterior uveitis are unknown, but ZIKV has been identified in the aqueous humor of affected individuals. Read More

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Tetraspanins as Potential Therapeutic Candidates for Targeting Flaviviruses.

Front Immunol 2021 21;12:630571. Epub 2021 Apr 21.

Department of Biological Sciences, Old Dominion University, Norfolk, VA, United States.

Tetraspanin family of proteins participates in numerous fundamental signaling pathways involved in viral transmission, virus-specific immunity, and virus-mediated vesicular trafficking. Studies in the identification of novel therapeutic candidates and strategies to target West Nile virus, dengue and Zika viruses are highly warranted due to the failure in development of vaccines. Recent evidences have shown that the widely distributed tetraspanin proteins may provide a platform for the development of novel therapeutic approaches. Read More

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[Vector management of arbovirosis cases notified in the city of Valencia, Spain (2016-2018).]

Rev Esp Salud Publica 2021 May 10;95. Epub 2021 May 10.

Servicio de Sanidad y Consumo. Ayuntamiento de València. Valencia. España.

Objective: Environmental management of imported arboviruses such as dengue (DENV), Zika (ZIKV) or Chikungunya (CHIKV) is a task of great significance for Public Health since the arrival and establishment of the competent vector Aedes albopictus (Asian tiger mosquito) in numerous cities of our country. There are recent examples of autochthonous transmission of some of these arboviruses in Spain probably linked to undetected and / or unmanaged imported cases.

Methods: Vector management interventions were carried out in the city of Valencia (Spain) by the Health Service of the Valencia City Council between 2016 and 2018. Read More

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Identifying urban hotspots of dengue, chikungunya, and Zika transmission in Mexico to support risk stratification efforts: a spatial analysis.

Lancet Planet Health 2021 May;5(5):e277-e285

Department of Environmental Sciences, Emory University, Atlanta, GA, USA. Electronic address:

Background: Effective Aedes aegypti control is limited, in part, by the difficulty in achieving sufficient intervention coverage. To maximise the effect of vector control, areas with persistently high numbers of Aedes-borne disease cases could be identified and prioritised for preventive interventions. We aimed to identify persistent Aedes-borne disease hotspots in cities across southern Mexico. Read More

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Limited flavivirus cross-reactive antibody responses elicited by a ZIKV DNA vaccine candidate in humans.

J Infect Dis 2021 May 7. Epub 2021 May 7.

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

ZIKV DNA vaccine VRC5283 encoding viral structural genes, has been shown to be immunogenic in humans. Recognizing that antigenically-related flaviviruses co-circulate in regions with ZIKV activity, we explored the degree of antibody cross-reactivity elicited by this vaccine candidate using genetically-diverse flaviviruses. The antibody response of vaccinated individuals with no evidence of prior flavivirus infection or vaccine experience had a limited capacity to bind heterologous viruses. Read More

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Guillain-Barré syndrome associated with SARS-CoV-2 infection: A case report with long term follow up.

J Neuroimmunol 2021 Apr 28;356:577590. Epub 2021 Apr 28.

Laboratory of Gut-Brain Signaling, Laboratory Sciences and Services Division, icddr,b, Dhaka 1212, Bangladesh. Electronic address:

A 50-years old male presented with quadriplegia and paresthesia and was diagnosed as Guillain-Barré syndrome (GBS). He was found positive for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) six weeks prior to the onset of weakness. GBS disability score was 4. Read More

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Identification of juvenile hormone-induced posttranslational modifications of methoprene tolerant and Krüppel homolog 1 in the yellow fever mosquito, Aedes aegypti.

J Proteomics 2021 May 3:104257. Epub 2021 May 3.

Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, S-225 Agricultural Science Center North Lexington, KY 40546-0091, United States. Electronic address:

Recent studies reported that JH regulated phosphorylation status of the JH-receptor complex contributes to its transcription activity in Aedes aegypti. However, phosphorylation sites of these proteins have not yet been identified. In this study, we found that the fusion of an EGFP tag to Ae. Read More

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Therapeutic targets and biological mechanisms of action of curcumin against Zika virus: in silico and in vitro analyses.

Eur J Pharmacol 2021 May 3:174144. Epub 2021 May 3.

Department of Biomedical Science and Engineering, Konkuk University, Seoul, 05029, Republic of Korea. Electronic address:

Zika virus (ZIKV) is a mosquito-borne flavivirus, that could cause congenital Zika syndrome (CZS), characterized by microcephaly, neurological complications and fetal deaths. No specific treatments for ZIKV are currently available, highlighting the urgent global need to identify and develop therapeutic agents. Drug repositioning of approved natural compounds can provide effective alternative solutions for novel antiviral development. Read More

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Factors associated with increased odds of sensorineural hearing loss in infants exposed to the Zika virus during pregnancy.

J Infect Dev Ctries 2021 Apr 30;15(4):590-594. Epub 2021 Apr 30.

Faculty of Civil Engineering, University of Colima, Coquimatlan, Mexico.

Introduction: The Zika virus (ZIKV) infection in pregnant women has been associated with an increased risk of birth defects. We aimed to estimate the prevalence of sensorineural hearing loss (SNHL) in infants exposed to the ZIKV during their gestation and evaluate the factors associated with its increased odds.

Methodology: A cross-sectional study was performed from July 2016 to June 2019 in a Western state of Mexico and data from 61 infants that presented with laboratory-positive (RT-qPCR) evidence of in utero exposure to ZIKV were analyzed. Read More

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AXL, an Important Host Factor for DENV and ZIKV Replication.

Front Cell Infect Microbiol 2021 22;11:575346. Epub 2021 Mar 22.

Ministry of Education (MOE) Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Flaviviruses, as critically important pathogens, are still major public health problems all over the world. For instance, the evolution of ZIKV led to large-scale outbreaks in the Yap island in 2007. DENV was considered by the World Health Organization (WHO) as one of the 10 threats to global health in 2019. Read More

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Repeated exposure to dengue virus elicits robust cross neutralizing antibodies against Zika virus in residents of Northeastern Thailand.

Sci Rep 2021 May 5;11(1):9634. Epub 2021 May 5.

Molecular Biotechnology Laboratory, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.

Zika virus (ZIKV) and dengue virus (DENV) are antigenically related mosquito-borne flaviviruses. ZIKV is becoming increasingly prevalent in DENV-endemic regions, raising the possibility that pre-existing immunity to one virus could modulate the response to a heterologous virus, although whether this would be beneficial or detrimental is unclear. Here, we analyzed sera from residents of a DENV-endemic region of Thailand to determine the prevalence of DENV-elicited antibodies capable of cross-neutralizing ZIKV. Read More

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Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase.

Nat Commun 2021 05 5;12(1):2530. Epub 2021 May 5.

Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX, USA.

Flaviviruses use a ~70 nucleotide stem-loop structure called stem-loop A (SLA) at the 5' end of the RNA genome as a promoter for RNA synthesis. Flaviviral polymerase NS5 specifically recognizes SLA to initiate RNA synthesis and methylate the 5' guanosine cap. We report the crystal structures of dengue (DENV) and Zika virus (ZIKV) SLAs. Read More

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Meningoencephalitis associated with Zika virus and Chikungunya virus infection.

Jpn J Infect Dis 2021 Apr 30. Epub 2021 Apr 30.

Laboratory of Virology, Health Sciences Institute, Universidade Federal da Bahia, Brazil.

Etiology of viral meningoencephalitis is frequently unidentified. Chikungunya virus (CHIKV) and Zika virus (ZIKV) have been known to affect the central nervous system, and should, therefore, be considered for diagnosis of meningoencephalitis since its outcome may be influenced by the etiologic agent, age and immunological condition of the patient. In this study we aimed to determine if CHIKV and ZIKV were the etiological agent of viral encephalitis in meningoencephalitis patients admitted to the main hospital of infectious disease in the city of Salvador, Brazil. Read More

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Zika Prevention Behaviors Among Women of Reproductive Age in Puerto Rico, 2016.

Am J Prev Med 2021 May 2. Epub 2021 May 2.

Division of Reproductive Health, Centers for Disease Control and Prevention, Atlanta, Georgia.

Introduction: Zika virus is primarily transmitted through mosquito bites. Because Zika virus infection during pregnancy can cause serious birth defects, reproductive-aged women need protection from Zika virus infection. This report describes Zika virus prevention behaviors among women aged 18-49 years and assesses whether pregnancy status and healthcare provider counseling increases Zika virus prevention behaviors. Read More

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Global patterns of aegyptism without arbovirus.

PLoS Negl Trop Dis 2021 May 5;15(5):e0009397. Epub 2021 May 5.

Department of Entomology, Texas A&M University, College Station, Texas, United States of America.

The world's most important mosquito vector of viruses, Aedes aegypti, is found around the world in tropical, subtropical and even some temperate locations. While climate change may limit populations of Ae. aegypti in some regions, increasing temperatures will likely expand its territory thus increasing risk of human exposure to arboviruses in places like Europe, Northern Australia and North America, among many others. Read More

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Use of Cerebrospinal Fluid for the Diagnosis of Neuroinvasive Dengue, Zika, and Chikungunya: A 19-year systematic review.

Rev Soc Bras Med Trop 2021 28;54:e0891 2020. Epub 2021 Apr 28.

Universidade Federal do Rio de Janeiro, Programa de Pós-Graduação em Doenças Infecciosas e Parasitárias, Rio de Janeiro, RJ, Brasil.

Introduction: Cerebrospinal fluid analysis contributes to the diagnosis and neuropathogenesis of neuroinvasive arboviruses. Neurological complications caused by dengue, Zika, and chikungunya infections have high clinical relevance because of their high potential to cause death or neurological deficits. We aimed to evaluate the use of cerebrospinal fluid assays for diagnostic support in neurological disorders associated with dengue, chikungunya, and Zika infections. Read More

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Enhanced electrochemiluminescence of CdS quantum dots capped with mercaptopropionic acid activated by EDC for Zika virus detection.

Analyst 2021 May;146(9):2928-2935

Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.

Enhanced electrochemiluminescence (ECL) signals of CdS quantum dots capped with 3-mercaptopropionic acid (MPA@CdS QDs) have been observed after using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) to activate the carboxyl groups. The generated ECL signals are strong enough that their images can be captured using a Huawei mobile phone. A possible mechanism for the generation of enhanced ECL signals has been proposed. Read More

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SARS-CoV-2-Associated Guillain-Barre Syndrome Obscured by Diabetes Mellitus Peripheral Neuropathy.

Cureus 2021 Mar 31;13(3):e14209. Epub 2021 Mar 31.

Internal Medicine, Medical College of Wisconsin, Milwaukee, USA.

Multiple neurological complications, including Guillain-Barre syndrome (GBS), have been reported in association with the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak. GBS has well-known associations with viruses such as influenza, human immunodeficiency virus, Zika, severe acute respiratory syndrome, Middle East respiratory syndrome, Epstein-Barr virus, and cytomegalovirus. Till date, there have been around 50 distinct published cases of GBS occurring concurrently or shortly after SARS-CoV-2 infection. Read More

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Effects of changes in temperature on Zika dynamics and control.

J R Soc Interface 2021 May 5;18(178):20210165. Epub 2021 May 5.

Department of Global Health and Social Medicine, Harvard Medical School, Boston, MA 02115, USA.

When a rare pathogen emerges to cause a pandemic, it is critical to understand its dynamics and the impact of mitigation measures. We use experimental data to parametrize a temperature-dependent model of Zika virus (ZIKV) transmission dynamics and analyse the effects of temperature variability and control-related parameters on the basic reproduction number () and the final epidemic size of ZIKV. Sensitivity analyses show that these two metrics are largely driven by different parameters, with the exception of temperature, which is the dominant driver of epidemic dynamics in the models. Read More

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Ocular Findings and Visual Function in Children Examined during the Zika Health Brigade in the US Virgin Islands, March 2018.

Trop Med Infect Dis 2021 Apr 29;6(2). Epub 2021 Apr 29.

United States Virgin Islands Department of Health, Christiansted, VI 00820, USA.

Among children born with laboratory-confirmed Zika virus (ZIKV) infection, visual impairment (VI) can occur despite normal ocular structure. The objective of this report is to describe ocular findings and visual function among children examined during the Department of Health Zika Health Brigade (ZHB) in the United States Virgin Islands in March 2018. This analysis is based on a retrospective chart review of children eligible to participate in the ZHB (i. Read More

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The Safe Baculovirus-Based PrM/E DNA Vaccine Protected Fetuses Against Zika Virus in A129 Mice.

Vaccines (Basel) 2021 Apr 30;9(5). Epub 2021 Apr 30.

Department of Bioindustrial Technologies, Konkuk University, Seoul 05029, Korea.

The Zika virus (ZIKV) is a mosquito-borne member of the family of enveloped RNA viruses. The correlation between viral infection and fetal microcephaly was revealed in 2015, yet we still lack a vaccine against ZIKV. Here, we present a genetic vaccine that delivers the premembrane (prM) and envelope (E) genes of ZIKV using a recombinant baculovirus vector that expresses a human endogenous retrovirus (HERV) envelope on its surface to enhance gene delivery. Read More

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Analysis of a Routinely Used Commercial Anti-Chikungunya IgM ELISA Reveals Cross-Reactivities with Dengue in Brazil: A New Challenge for Differential Diagnosis?

Diagnostics (Basel) 2021 Apr 30;11(5). Epub 2021 Apr 30.

Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro 21040-900, Brazil.

In Brazil, chikungunya emerged in 2014, and by 2016, co-circulated with other arbovirosis, such as dengue and zika. ELISAs (Enzyme-Linked Immunosorbent Assays) are the most widely used approach for arboviruses diagnosis. However, some limitations include antibody cross reactivities when viruses belong to the same genus, and sensitivity variations in distinct epidemiological scenarios. Read More

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Trimerization of the N-Terminal Tail of Zika Virus NS4A Protein: A Potential In Vitro Antiviral Screening Assay.

Membranes (Basel) 2021 Apr 30;11(5). Epub 2021 Apr 30.

School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.

The nonstructural (NS) protein NS4A in flaviviruses is a membrane protein that is critical for virulence, and, among other roles, it participates in membrane morphogenesis. In dengue virus (DENV), the NS4A hydrophilic N-terminal tail, together with the first transmembrane domain, is involved in both homo-oligomerization and hetero-oligomerization with NS4B. In both DENV and Zika virus (ZIKV), this N-terminal tail (residues 1-48) forms a random coil in solution but becomes mostly α-helical upon interaction with detergents or lipid membranes. Read More

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Co-Circulation of All Four Dengue Viruses and Zika Virus in Guerrero, Mexico, 2019.

Vector Borne Zoonotic Dis 2021 Apr 30. Epub 2021 Apr 30.

College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.

A clinical and entomological investigation was performed to identify flavivirus infections in humans and mosquitoes in impoverished areas of Guerrero, a coastal state in southwestern Mexico. A total of 639 patients with acute febrile illness and 830 resting female mosquitoes in low-income communities of Guerrero in 2019 were tested for evidence of flavivirus infection. Sera were collected from all patients and screened at a dilution of 1:20 by plaque reduction neutralization test (PRNT) using dengue virus (DENV)2. Read More

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