1,381 results match your criteria zebrafish interestingly

The conserved fertility factor SPACA4/Bouncer has divergent modes of action in vertebrate fertilization.

Proc Natl Acad Sci U S A 2021 09;118(39)

Research Institute of Molecular Pathology, Vienna BioCenter, Campus-Vienna-Biocenter 1, 1030 Vienna, Austria;

Fertilization is the fundamental process that initiates the development of a new individual in all sexually reproducing species. Despite its importance, our understanding of the molecular players that govern mammalian sperm-egg interaction is incomplete, partly because many of the essential factors found in nonmammalian species do not have obvious mammalian homologs. We have recently identified the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) protein Bouncer as an essential fertilization factor in zebrafish [S. Read More

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September 2021

HMGA2 contributes to vascular development and sprouting angiogenesis by promoting IGFBP2 production.

Exp Cell Res 2021 Sep 18:112831. Epub 2021 Sep 18.

Institute for Developmental and Regenerative Cardiovascular Medicine, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China; Department of Pediatric Cardiovascular, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China. Electronic address:

Angiogenesis is the process by which new blood vessels form from preexisting vessels and regulates the processes of embryonic development, wound healing and tumorigenesis. HMGA2 is involved in the occurrence of several cancers, but its biological role and the exact downstream genes involved in vascular development and sprouting angiogenesis remain largely unknown. Here, we first found that HMGA2 knockdown in zebrafish embryos resulted in defects of central artery formation. Read More

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September 2021

Novel zebrafish polycystic kidney disease (PKD) models reveal functions of the Hippo pathway in renal cystogenesis.

Dis Model Mech 2021 Sep 21. Epub 2021 Sep 21.

Department of Biomedical Sciences and Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau, China.

Hippo signaling pathway is a kinase cascade which plays an important role in organ size control. As the main effectors of the Hippo pathway, transcription coactivators Yap1/Wwtr1 are regulated by the upstream kinase Stk3. Recent studies in mammals have implicated Hippo pathway in kidney development and kidney diseases. Read More

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September 2021

Loss of mpv17 affected early embryonic development via mitochondria dysfunction in zebrafish.

Cell Death Discov 2021 Sep 18;7(1):250. Epub 2021 Sep 18.

School of Public Health and Management, Chongqing Medical University, 400016, Chongqing, China.

MVP17 encodes a mitochondrial inner-membrane protein, and mutation of human MVP17 can cause mitochondria DNA depletion syndrome (MDDS). However, the underlying function of mpv17 is still elusive. Here, we developed a new mutant with mpv17 knockout by using the CRISPR/Cas9 system. Read More

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September 2021

Germline and Somatic Variants Convergently Promote Endothelial-to-Mesenchymal Transition in Simplex Brain Arteriovenous Malformation.

Circ Res 2021 Sep 17. Epub 2021 Sep 17.

Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, CHINA.

Brain arteriovenous malformations (bAVMs) are abnormal entanglement of blood vessels in brain, with direct connections from arteries to veins, lacking functional capillary bed. Although several somatic mutations were reported, the molecular mechanism and genetic disposition of bAVM remain poorly understood. We aim to identify transcriptional anomalies and critical functional pathways in bAVM lesions, and explore their association with key de novo germline and somatic variants in bAVM patients. Read More

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September 2021

Bis(2-ethylhexyl)-tetrabromophthalate induces zebrafish obesity by altering the brain-gut axis and intestinal microbial composition.

Environ Pollut 2021 Sep 6;290:118127. Epub 2021 Sep 6.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.

Multiple environmental stressors, including chemicals termed obesogens, contribute to the susceptibility of organisms to obesity. Bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate (TBPH), a novel brominated flame retardant, is an environmental contaminant that may disrupt lipid metabolism. However, the risk of TBPH leading to obesity remains unknown. Read More

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September 2021

Pipeline for generating stable large genomic deletions in zebrafish, from small domains to whole gene excisions.

G3 (Bethesda) 2021 Sep 9. Epub 2021 Sep 9.

The University of Queensland, Queensland Brain Institute, Brisbane, Queensland, 4072, Australia.

Here we describe a short feasibility study and methodological framework for the production of stable, CRISPR/Cas9-based, large genomic deletions in zebrafish, ranging from several base pairs (bp) to hundreds of kilobases (kb). Using a cocktail of four sgRNAs targeting a single genomic region mixed with a marker-sgRNA against the pigmentation gene tyrosinase (tyr), we demonstrate that one can easily and accurately excise genomic regions such as promoters, protein domains, specific exons or whole genes. We exemplify this technique with a complex gene family, neurexins, composed of three duplicated genes with multiple promoters and intricate splicing processes leading to thousands of isoforms. Read More

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September 2021

Redundant mechanisms driven independently by RUNX1 and GATA2 for hematopoietic development.

Blood Adv 2021 Sep 7. Epub 2021 Sep 7.

NHGRI, NIH, Bethesda, Maryland, United States.

RUNX1 is essential for the generation of hematopoietic stem cells (HSCs). Runx1 null mouse embryos lack definitive hematopoiesis and die in mid-gestation. However, even though zebrafish embryos with a runx1 W84X mutation have defects in early definitive hematopoiesis, some runx1W84X/W84X embryos can develop to fertile adults with blood cells of multi-lineages, raising the possibility that HSCs can emerge without RUNX1. Read More

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September 2021

Glutamate 73 Promotes Anti-arrhythmic Effects of Voltage-Dependent Anion Channel Through Regulation of Mitochondrial Ca Uptake.

Front Physiol 2021 18;12:724828. Epub 2021 Aug 18.

Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, United States.

Mitochondria critically regulate a range of cellular processes including bioenergetics, cellular metabolism, apoptosis, and cellular Ca signaling. The voltage-dependent anion channel (VDAC) functions as a passageway for the exchange of ions, including Ca, across the outer mitochondrial membrane. In cardiomyocytes, genetic or pharmacological activation of isoform 2 of VDAC (VDAC2) effectively potentiates mitochondrial Ca uptake and suppresses Ca overload-induced arrhythmogenic events. Read More

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COPB2 loss of function causes a coatopathy with osteoporosis and developmental delay.

Am J Hum Genet 2021 09 26;108(9):1710-1724. Epub 2021 Aug 26.

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.

Coatomer complexes function in the sorting and trafficking of proteins between subcellular organelles. Pathogenic variants in coatomer subunits or associated factors have been reported in multi-systemic disorders, i.e. Read More

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September 2021

An irradiated marrow niche reveals a small non-collagenous protein mediator of homing, dermatopontin.

Blood Adv 2021 Aug 27. Epub 2021 Aug 27.

University of Minnesota, Minneapolis, Minnesota, United States.

Hematopoietic cell homing after hematopoietic cell transplant (HCT) is governed by several pathways involving marrow niche cells that are evoked after pre-HCT conditioning. To understand the factors that play a role in homing, we performed expression analysis on the zebrafish marrow niche cells following conditioning. We determined that the non-collagenous protein extracellular matrix related protein dermatopontin (Dpt) was upregulated seven-fold in response to irradiation. Read More

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Early suppression of the endocannabinoid degrading enzymes FAAH and MAGL alters locomotor development in zebrafish.

J Exp Biol 2021 08 26;224(16). Epub 2021 Aug 26.

Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada.

The fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) enzymes are the predominant catabolic regulators of the major endocannabinoids (eCBs) anadamide (AEA) and 2-arachidonoylglycerol (2-AG), respectively. The expression and roles of eCBs during early embryogenesis remain to be fully investigated. Here, we inhibited FAAH and MAGL in zebrafish embryos during the first 24 h of life and examined motor neuron and locomotor development at 2 and 5 days post fertilization (dpf). Read More

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The Zebrafish Meiotic Cohesin Complex Protein Smc1b Is Required for Key Events in Meiotic Prophase I.

Front Cell Dev Biol 2021 9;9:714245. Epub 2021 Aug 9.

Biology Department, University of Massachusetts Boston, Boston, MA, United States.

The eukaryotic structural maintenance of chromosomes (SMC) proteins are involved in key processes of chromosome structure and dynamics. SMC1β was identified as a component of the meiotic cohesin complex in vertebrates, which aids in keeping sister chromatids together prior to segregation in meiosis II and is involved in association of homologous chromosomes in meiosis I. The role of SMC1β in meiosis has primarily been studied in mice, where mutant male and female mice are infertile due to germ cell arrest at pachytene and metaphase II stages, respectively. Read More

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Evolution of lbx spinal cord expression and function.

Evol Dev 2021 Sep 19;23(5):404-422. Epub 2021 Aug 19.

Department of Biology, Syracuse University, Syracuse, New York, USA.

Ladybird homeobox (Lbx) transcription factors have crucial functions in muscle and nervous system development in many animals. Amniotes have two Lbx genes, but only Lbx1 is expressed in spinal cord. In contrast, teleosts have three lbx genes and we show here that zebrafish lbx1a, lbx1b, and lbx2 are expressed by distinct spinal cell types, and that lbx1a is expressed in dI4, dI5, and dI6 interneurons, as in amniotes. Read More

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September 2021

Modeling Neurodevelopmental Disorders and Epilepsy Caused by Loss of Function of in Zebrafish.

eNeuro 2021 Sep-Oct;8(5). Epub 2021 Sep 7.

Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, 3000 Leuven, Belgium.

In recent years there has been extensive research on malformations of cortical development (MCDs) that result in clinical features like developmental delay, intellectual disability, and drug-resistant epilepsy (DRE). Various studies highlighted the contribution of microtubule-associated genes (including tubulin and kinesin encoding genes) in MCD development. It has been reported that mutations in , a member of the family, are linked to brain malformations and DRE. Read More

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September 2021

Cadmium significantly changes major morphometrical points and cardiovascular functional parameters during early development of zebrafish.

Environ Toxicol Pharmacol 2021 Oct 12;87:103723. Epub 2021 Aug 12.

Department of Pathophysiology, Medical Faculty, University of Belgrade, Belgrade, Serbia. Electronic address:

Living organisms are commonly exposed to cadmium and other toxic metals. A vast body of research has shown the significant effects of these toxic metals on developmental processes. In order to study the role of toxic metals on early developmental stages of eukaryotes, we explored the effect of cadmium (Cd) contaminant on zebrafish. Read More

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October 2021

Temporal Patterns in Interactions Across Same- and Mixed-Sex Wild Zebrafish Dyads.

Zebrafish 2021 Aug 10. Epub 2021 Aug 10.

Department of Biological Sciences, Indian Institute of Science Education and Research, Kolkata, India.

Animals communicate with each other through a variety of behavioral interactions, many of which are often complex due to the interplay of several ecological factors. Observations on dyadic interactions can help throw light on the more complex interactions observed among group living organisms and can help in understanding mechanisms of behaviors related to mating strategies, dominance hierarchies, and decision-making. This study focused on the assessment of several generally observed interactions among dyads of different sexes (female-female, male-male, and male-female) in wild zebrafish (. Read More

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Nanoplastics Induce More Serious Microbiota Dysbiosis and Inflammation in the Gut of Adult Zebrafish than Microplastics.

Bull Environ Contam Toxicol 2021 Aug 11. Epub 2021 Aug 11.

College of Marine Science, South China Agricultural University, Guangzhou, Guangdong, 510642, PR China.

Microplastics (MPs) (< 5 mm) and nanoplastics (NPs) (< 100 nm) are emerging environmental pollutants and have been proved could cause a series of toxicity in aquatic organisms. In this study, the effects on gut microbiota of adult zebrafish exposed for 21 days to 10 μg/L and 1 mg/L of MPs (8 μm) and NPs (80 nm) were evaluated. We analyzed the intestinal microbial community of zebrafish using high throughput sequencing of the 16S rRNA gene V3-V4 region and also performed transcriptional profiling of the inflammation pathway related genes in the intestinal tissues. Read More

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MiR-107 inhibits the sprouting of intersegmental vessels of zebrafish embryos.

Protoplasma 2021 Aug 9. Epub 2021 Aug 9.

Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.

MicroRNAs (miRNAs) play important roles in various biological processes. Our previous study showed that inhibition of MTOR with rapamycin treatment suppressed human endothelial cell tube formation, concomitant with the down-regulation of miR-107. Similarly, inhibition of Ztor by rapamycin also suppressed vascular development in zebrafish embryos. Read More

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The effect of fascin 1 inhibition on head and neck squamous cell carcinoma cells.

Eur J Oral Sci 2021 Aug 4. Epub 2021 Aug 4.

Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, Helsinki, Finland.

Fascin 1 plays important pro-metastatic roles in head and neck carcinoma (HNSCC) migration, invasion, and metastasis. However, limited advancement in targeting metastasis remains a major obstacle in improving HNSCC patients' survival. Therefore, we assessed the therapeutic potential of fascin 1 targeted inhibition and its potential prognostic value in HNSCC patients. Read More

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Zebrafish as a new model for rhododendrol-induced leukoderma.

Pigment Cell Melanoma Res 2021 Jul 26. Epub 2021 Jul 26.

Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan.

Idiopathic leukoderma is a skin disorder characterized by patchy loss of skin pigmentation due to melanocyte dysfunction or deficiency. Rhododendrol (RD) was approved as a cosmetic ingredient in Japan in 2008. However, it was shown to induce leukoderma in approximately 20,000 customers. Read More

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Loss of αklotho causes reduced motor ability and short lifespan in zebrafish.

Sci Rep 2021 Jul 23;11(1):15090. Epub 2021 Jul 23.

Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, Sagamihara, 252-5258, Japan.

The klotho gene encodes a transmembrane protein αKlotho that interacts with a fibroblast growth factor (FGF) receptor in renal tubular epithelial cells and functions as a co-receptor for FGF23, which is an osteocytes-derived hormone. This bone-to-kidney signal promotes urinary phosphate excretion. Interestingly, αKlotho knockout mice show an accelerated aging and a shortened life span. Read More

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Genetic Modeling of the Neurodegenerative Disease Spinocerebellar Ataxia Type 1 in Zebrafish.

Int J Mol Sci 2021 Jul 8;22(14). Epub 2021 Jul 8.

Division of Cellular & Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany.

Dominant spinocerebellar ataxias (SCAs) are progredient neurodegenerative diseases commonly affecting the survival of Purkinje cells (PCs) in the human cerebellum. Spinocerebellar ataxia type 1 (SCA1) is caused by the mutated () gene product, in which a polyglutamine stretch encoded by CAG repeats is extended in affected SCA1 patients. As a monogenetic disease with the Atx1-polyQ protein exerting a gain of function, SCA1 can be genetically modelled in animals by cell type-specific overexpression. Read More

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Fulvic acid accelerates hatching and stimulates antioxidative protection and the innate immune response in zebrafish larvae.

Sci Total Environ 2021 Nov 6;796:148780. Epub 2021 Jul 6.

Department of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, 12587 Berlin, Germany; Humboldt University of Berlin, Faculty of Life Sciences, 10115 Berlin, Germany.

Aquaculture plays a pivotal role in covering dietary animal protein demands and restocking endangered fish populations. However, high mortality takes place at the earliest life stages: prior and immediately after hatching. Improving growth and health parameters by immunostimulants is widely used in older fish, but rarely studied in larvae. Read More

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November 2021

Injury-induced Autophagy Delays Axonal Regeneration after Optic Nerve Damage in Adult Zebrafish.

Neuroscience 2021 08 17;470:52-69. Epub 2021 Jul 17.

Neural Circuit Development and Regeneration Research Group, Animal Physiology and Neurobiology Section, Department of Biology, KU Leuven, 3000 Leuven, Belgium; Leuven Brain Institute, 3000 Leuven, Belgium. Electronic address:

Optic neuropathies comprise a group of disorders in which the axons of retinal ganglion cells (RGCs), the retinal projection neurons conveying visual information to the brain, are damaged. This results in visual impairment or even blindness, which is irreversible as adult mammals lack the capacity to repair or replace injured or lost neurons. Despite intensive research, no efficient treatment to induce axonal regeneration in the central nervous system (CNS) is available yet. Read More

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Defective Neuronal Positioning Correlates With Aberrant Motor Circuit Function in Zebrafish.

Front Neural Circuits 2021 24;15:690475. Epub 2021 Jun 24.

Division of Biological Sciences and Bond Life Sciences Center, University of Missouri, Columbia, MO, United States.

Precise positioning of neurons resulting from cell division and migration during development is critical for normal brain function. Disruption of neuronal migration can cause a myriad of neurological disorders. To investigate the functional consequences of defective neuronal positioning on circuit function, we studied a zebrafish () loss-of-function mutant () where the facial branchiomotor (FBM) neurons fail to migrate out of their birthplace. Read More

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Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain.

Fish Physiol Biochem 2021 Aug 8;47(4):1283-1298. Epub 2021 Jul 8.

Department of Organismal Biology, Comparative Physiology, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18A, SE-752 36, Uppsala, Sweden.

The signal mediated by leptin hormone and its receptor is a major regulator of body weight, food intake and metabolism. In mammals and many teleost fish species, leptin has an anorexigenic role and inhibits food intake by influencing the appetite centres in the hypothalamus. However, the regulatory connections between leptin and downstream genes mediating its appetite-regulating effects are still not fully explored in teleost fish. Read More

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Cryptotanshinone Inhibits the Growth of HCT116 Colorectal Cancer Cells Through Endoplasmic Reticulum Stress-Mediated Autophagy.

Front Pharmacol 2021 17;12:653232. Epub 2021 Jun 17.

School of Basic Medicine, Qingdao University, Qingdao, China.

Among cancers, colorectal cancer (CRC) has one of the highest annual incidence and death rates. Considering severe adverse reactions associated with classical chemotherapy medications, traditional Chinese medicines have become potential drug candidates. In the current study, the effects of cryptotanshinone (CPT), a major component of () on CRC and underlying mechanism were explored. Read More

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Evaluation of Dexamethasone-Induced Osteoporosis In Vivo Using Zebrafish Scales.

Pharmaceuticals (Basel) 2021 Jun 3;14(6). Epub 2021 Jun 3.

Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan.

Glucocorticoid-induced osteoporosis (GIOP) is a major cause of secondary osteoporosis, and the pathogenic mechanisms of GIOP remain to be elucidated. Here, we show a rapid dexamethasone-induced osteoporosis animal model using zebrafish scales. Intraperitoneal injection of dexamethasone over a 5-day period suppressed the regeneration of scales. Read More

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Vimentin Promotes the Aggressiveness of Triple Negative Breast Cancer Cells Surviving Chemotherapeutic Treatment.

Cells 2021 Jun 15;10(6). Epub 2021 Jun 15.

UMR9020-U1277-CANTHER-Cancer Heterogeneity, Plasticity and Resistance to Therapies, University of Lille, CNRS, Inserm, CHU Lille, F-59000 Lille, France.

Tremendous data have been accumulated in the effort to understand chemoresistance of triple negative breast cancer (TNBC). However, modifications in cancer cells surviving combined and sequential treatment still remain poorly described. In order to mimic clinical neoadjuvant treatment, we first treated MDA-MB-231 and SUM159-PT TNBC cell lines with epirubicin and cyclophosphamide for 2 days, and then with paclitaxel for another 2 days. Read More

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