16,007 results match your criteria zebrafish embryos


Sublethal Effect Concentrations for Non-Polar Narcosis in the Zebrafish Embryo.

Environ Toxicol Chem 2021 Jul 20. Epub 2021 Jul 20.

Zebrafishlab, Veterinary, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610, Wilrijk, Belgium.

Non-polar narcosis, also known as baseline toxicity, has been described as the minimal toxicity that an organic chemical may elicit based on its lipophilicity. While lethal effects of narcosis-inducing chemicals (NICs) have been thoroughly investigated, knowledge of sublethal effects is still very limited. We investigated the effects of three well-known NICs (phenanthrene, 1,3,5-trichlorobenzene and pentachlorobenzene) on a variety of organismal endpoints (malformations, swim bladder inflation, respiration, heart rate, swimming activity and turning angles), which can be plausibly linked to narcosis in zebrafish embryos. Read More

View Article and Full-Text PDF

24-Epibrassinolide modulates the neurodevelopmental outcomes of high caffeine exposure in zebrafish (Danio rerio) embryos.

Comp Biochem Physiol C Toxicol Pharmacol 2021 Jul 17:109143. Epub 2021 Jul 17.

Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal; Veterinary and Animal Science Research Center (CECAV), University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal; Department of Animal Science, School of Agrarian and Veterinary Sciences (ECAV), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal.

Previous embryonic fish data have shown caffeine to induce potential teratogenic and long-term neurodevelopmental outcomes through oxidative stress-mediated apoptosis. In this context, antioxidants may have the potential to counteract the caffeine-induced effects. Therefore, the present study aimed to investigate the potential protective role of 24-epibrassinolide (24-EPI), a natural brassinosteroid with proven antioxidant properties, against caffeine-induced teratogenic effects during early zebrafish development. Read More

View Article and Full-Text PDF

Cardiac toxicity assessment of pendimethalin in zebrafish embryos.

Ecotoxicol Environ Saf 2021 Jul 17;222:112514. Epub 2021 Jul 17.

Ganzhou Key Laboratory for Drug Screening and Discovery, School of Geography and Environmental Engineering, Gannan Normal University, China; Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Ji'an 343009, Jiangxi, China; Jiangxi Key Laboratory of Developmental Biology of Organs, Ji'an 343009, Jiangxi, China. Electronic address:

Pendimethalin (PND) is one of the best sellers of selective herbicide in the world and has been frequently detected in the water. However, little is known about its effects on cardiac development. In this study, we used zebrafish to investigate the developmental and cardiac toxicity of PND. Read More

View Article and Full-Text PDF

Effects of 4-epianhydrotetracycline on oxidative stress in zebrafish (Danio rerio) embryos.

Sci Total Environ 2021 Jul 14;796:149047. Epub 2021 Jul 14.

College of Biology and Environmental Science, Jishou University, Jishou 416000, PR China.

4-Epianhydrotetracycline (4-EATC) is a major intermediate product of tetracycline during its degradation progress in the natural environment, which is frequently detected in aquatic environments and poses a potential threat to aquatic organisms. In the present study, the ecotoxicity of 4-EATC have been studied from the perspective of oxidative stress by using zebrafish embryos. After 96 h exposure, the reactive oxygen species (ROS) levels, malondialdehyde (MDA) concentrations and protein carbonyl (PC) contents in zebrafish embryos in the lower-concentration 4-EATC treatment groups (1. Read More

View Article and Full-Text PDF

A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish.

J Vis Exp 2021 Jun 30(172). Epub 2021 Jun 30.

Division of Developmental Biology, Cincinnati Children's Hospital Medical Center; Department of Pediatrics, University of Cincinnati College of Medicine; Department of Genetics, Albert Einstein College of Medicine.

Vertebrate embryos pattern their major body axis as repetitive somites, the precursors of vertebrae, muscle, and skin. Somites progressively segment from the presomitic mesoderm (PSM) as the tail end of the embryo elongates posteriorly. Somites form with regular periodicity and scale in size. Read More

View Article and Full-Text PDF

Accumulation and Distribution of Fluorescent Microplastics in the Early Life Stages of Zebrafish.

J Vis Exp 2021 Jul 4(173). Epub 2021 Jul 4.

College of Environment, Zhejiang University of Technology;

As a new type of environmental pollutant, microplastic has been widely found in the aquatic environment and poses a high threat to aquatic organisms. The bioaccumulation of microplastics plays a key role in their toxic effects; however, as a particulate, their bioaccumulations are different from many other pollutants. Described here is a feasible method to visually determine the accumulation and distribution of microplastics in zebrafish embryos or larvae using fluorescent microplastics. Read More

View Article and Full-Text PDF

3D viscoelastic drag forces drive changes to cell shapes during organogenesis in the zebrafish embryo.

Cells Dev 2021 Jul 14:203718. Epub 2021 Jul 14.

Department of Physics and BioInspired Syracuse: Institute for Material and Living Systems, Syracuse University, Syracuse, NY, USA. Electronic address:

The left-right organizer in zebrafish embryos, Kupffer's Vesicle (KV), is a simple organ that undergoes programmed asymmetric cell shape changes that are necessary to establish the left-right axis of the embryo. We use simulations and experiments to investigate whether 3D mechanical drag forces generated by the posteriorly-directed motion of the KV through the tailbud tissue are sufficient to drive such shape changes. We develop a fully 3D vertex-like (Voronoi) model for the tissue architecture, and demonstrate that the tissue can generate drag forces and drive cell shape changes. Read More

View Article and Full-Text PDF

The use of tocofersolan as a rescue agent in larval zebrafish exposed to benzo[a]pyrene in early development.

Neurotoxicology 2021 Jul 14;86:78-84. Epub 2021 Jul 14.

Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, USA. Electronic address:

Polycyclic aromatic hydrocarbons (PAHs) are widespread environmental pollutants created by incomplete combustion. Benzo(a)pyrene (BaP), the prototypic PAH, is known to exert toxicity through oxidative stress which is thought to occur through inhibition of antioxidant scavenging systems. The use of agents that reduce oxidative stress may be a valuable route for ameliorating the adverse effects of PAHs on neural development and behavior. Read More

View Article and Full-Text PDF

Species and Life Stage Sensitivity of Chinese Rare Minnow (Gobiocypris rarus) to Chemical Exposure: A Critical Review.

Environ Toxicol Chem 2021 Jul 15. Epub 2021 Jul 15.

School of Environment and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 510632, China.

Chemical production and consumption in Asia are increasing at an unprecedented rate, calling for advancing regulations for chemical management. Under the New Chemical Substance Notification in China, information on ecotoxicological effects of chemicals is mandatory for the simplified registrations of chemicals with the requirement that one ecotoxicological test is conducted locally. It is now mandatory to use the native fish species, Chinese rare minnow (Gobiocypris rarus). Read More

View Article and Full-Text PDF

Behavior of the Antibacterial Peptide Cyclo[RRRWFW], Explored Using a 3-Hydroxychromone-Derived Fluorescent Amino Acid.

Front Chem 2021 28;9:688446. Epub 2021 Jun 28.

Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.

Labeling biomolecules with fluorescent labels is an established tool for structural, biochemical, and biophysical studies; however, it remains underused for small peptides. In this work, an amino acid bearing a 3-hydroxychromone fluorophore, 2-amino-3-(2-(furan-2-yl)-3-hydroxy-4-oxo-4H-chromen-6-yl)propanoic acid (FHC), was incorporated in a known hexameric antimicrobial peptide, cyclo[RRRWFW] (cWFW), in place of aromatic residues. Circular dichroism spectropolarimetry and antibacterial activity measurements demonstrated that the FHC residue perturbs the peptide structure depending on labeling position but does not modify the activity of cWFW significantly. Read More

View Article and Full-Text PDF

Histopathological assessment of laterality defects in zebrafish development.

Anim Cells Syst (Seoul) 2021 26;25(3):136-145. Epub 2021 May 26.

Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon, Republic of Korea.

Laterality defects during embryonic development underlie the aetiology of various clinical symptoms of neuropathological and cardiovascular disorders; however, experimental approaches to understand the underlying mechanisms are limited due to the complex organ systems of vertebrate models. Zebrafish have the ability to survive even when the heart stops beating for a while during early embryonic development and those adults with cardiac abnormalities. Therefore, we induced laterality defects and investigated the occurrence of situs solitus, situs inversus, and situs ambiguus in zebrafish development. Read More

View Article and Full-Text PDF

Early, nonlethal ploidy and genome size quantification using confocal microscopy in zebrafish embryos.

J Exp Zool B Mol Dev Evol 2021 Jul 12. Epub 2021 Jul 12.

Department of Biology, University of New Brunswick, Fredericton, NB, Canada.

Ploidy transitions through whole genome duplication have shaped evolution by allowing the sub- and neo-functionalization of redundant copies of highly conserved genes to express novel traits. The nuclear:cytoplasmic (n:c) ratio is maintained in polyploid vertebrates resulting in larger cells, but body size is maintained by a concomitant reduction in cell number. Ploidy can be manipulated easily in most teleosts, and the zebrafish, already well established as a model system for biomedical research, is therefore an excellent system in which to study the effects of increased cell size and reduced cell numbers in polyploids on development and physiology. Read More

View Article and Full-Text PDF

Functional assessment of two variants of unknown significance in TEK by endothelium-specific expression in zebrafish embryos.

Hum Mol Genet 2021 Jul 12. Epub 2021 Jul 12.

Division of Pediatric Hematology and Oncology, Department of Pediatrics and Adolescent Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.

Vascular malformations are most often caused by somatic mutations of the PI3K/mTOR and the RAS signaling pathways, which can be identified in the affected tissue. Venous malformations commonly harbor PIK3CA and TEK mutations, whereas arteriovenous malformations are usually caused by BRAF, RAS, or MAP2K1 mutations. Correct identification of the underlying mutation is of increasing importance, since targeted treatments are becoming more and more relevant, especially in patients with extensive vascular malformations. Read More

View Article and Full-Text PDF

Developmental toxicity caused by sanguinarine in zebrafish embryos via regulating oxidative stress, apoptosis and wnt pathways.

Toxicol Lett 2021 Jul 9;350:71-80. Epub 2021 Jul 9.

Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Engineering Research Center of Zebrafish Models for Human Diseases and Drug Screening of Shandong Province, Shandong Provincial Engineering Laboratory for Biological Testing Technology, 28789 Jingshidong Road, Licheng District, Jinan, 250103, Shandong Province, PR China. Electronic address:

Sanguinarine, derived from the root of Sanguinaria canadensis, have multiple biological activities, such as antimicrobial, insecticidal, antitumor, anti-inflammatory and anti-angiogenesis effect, but little is known about its toxicity on normal embryonic development. Here, we study the developmental toxicity using zebrafish model. Notably, sanguinarine caused a significant increase of the malformation rate and decrease of hatching rates and body length of zebrafish embryos. Read More

View Article and Full-Text PDF

Venlafaxine deposition in the zygote disrupts the endocrine control of growth in juvenile zebrafish.

Environ Res 2021 Jul 10;202:111665. Epub 2021 Jul 10.

Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada. Electronic address:

The antidepressant venlafaxine can be found at levels nearing μg/L in waterways receiving municipal wastewater effluent, exposing non-target organisms, such as fish, to this chemical. We showed previously that zygotic exposure to venlafaxine alters neurodevelopment and behaviour in zebrafish (Danio rerio) larvae. Here, we tested the hypothesis that the zygotic deposition of venlafaxine disrupts endocrine pathways related to growth in zebrafish. Read More

View Article and Full-Text PDF

Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development.

Elife 2021 Jul 12;10. Epub 2021 Jul 12.

Centre for Organismal Studies Heidelberg, Heidelberg University, Heidelberg, Germany.

Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. Read More

View Article and Full-Text PDF

Developmental toxicity, antioxidant, and marker enzyme assessment of swertiamarin in zebrafish (Danio rerio).

J Biochem Mol Toxicol 2021 Jul 12:e22843. Epub 2021 Jul 12.

Department of Zoology, GTN Arts College, Dindigul, India.

A secoiridoid glycoside called swertiamarin has been widely used as a herbal medicine for many decades. In particular, swertiamarin from the Enicostema axillare herb has been used as a multipurpose drug to treat innumerable health problems. As this medicine is consumed orally, its toxicity level should be determined. Read More

View Article and Full-Text PDF

A versatile, automated and high-throughput drug screening platform for zebrafish embryos.

Biol Open 2021 Jul 9. Epub 2021 Jul 9.

Research Department of Haematology, Cancer Institute, University College London, London, UK.

Zebrafish provide a unique opportunity for drug screening in living animals, with the fast developing, transparent embryos allowing for relatively high-throughput, microscopy-based screens. However, the limited availability of rapid, flexible imaging and analysis platforms has limited the use of zebrafish in drug screens. We have developed an easy-to-use, customisable automated screening procedure suitable for high-throughput phenotype-based screens of live zebrafish. Read More

View Article and Full-Text PDF

Systematic identification of a panel of strong promoter regions from Listeria monocytogenes for fine-tuning gene expression.

Microb Cell Fact 2021 Jul 12;20(1):132. Epub 2021 Jul 12.

School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, People's Republic of China.

Background: Attenuated Listeria monocytogenes (Lm) has been widely used as a vaccine vector in the prevention and treatment of pathogen infection and tumor diseases. In addition, previous studies have proved that the attenuated Lm can protect zebrafish from Vibrio infections, indicating that the attenuated Lm has a good application prospect in the field of aquatic vaccines. However, the limitation mainly lies in the lack of a set of well-characterized natural promoters for the expression of target antigens in attenuated Lm. Read More

View Article and Full-Text PDF

Toxicity of polymer-modified CuS nanoclusters on zebrafish embryo development.

J Appl Toxicol 2021 Jul 11. Epub 2021 Jul 11.

School of Medicine, Nankai University, Tianjin, China.

Despite the vast amount of research on the toxicity of copper-based nanoparticles, the toxicity of CuS nanoparticles is still largely unknown. Due to the application of CuS-based nanomaterials in biomedical engineering, it is necessary to study their potential toxicity and biological effects. In this study, we evaluated the toxicity of polymer-modified CuS nanoclusters ([email protected]) on embryo development through exposing zebrafish embryos to 1, 2. Read More

View Article and Full-Text PDF

Antidiabetic drug metformin disrupts the embryogenesis in zebrafish through an oxidative stress mechanism.

Chemosphere 2021 Jul 7;285:131213. Epub 2021 Jul 7.

Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón intersección Paseo Tollocan, Colonia Residencial Colón, CP 50120, Toluca, Estado de, Mexico.

In recent years, the consumption of metformin has increased not only due to the higher prevalence of type 2 diabetes, but also due to their usage for other indications such as cancer and polycystic ovary syndrome. Consequently, metformin is currently among the highest drug by weight released into the aquatic environments. Since the toxic effects of this drug on aquatic species has been scarcely explored, the aim of this work was to investigate the influence of metformin on the development and redox balance of zebrafish (Danio rerio) embryos. Read More

View Article and Full-Text PDF

Microcystin-leucine arginine blocks vasculogenesis and angiogenesis through impairing cytoskeleton and impeding endothelial cell migration by downregulating integrin-mediated Rho/ROCK signaling pathway.

Environ Sci Pollut Res Int 2021 Jul 9. Epub 2021 Jul 9.

Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, No. 174, Shazheng Street, Shapingba District, Chongqing, 400030, People's Republic of China.

The main characteristic of eutrophication is cyanobacteria harmful algae blooms. Microcystin-leucine arginine (MC-LR) is considered to be the most toxic and most commonly secondary metabolite produced by cyanobacteria. It has been reported that MC-LR had potential vascular toxicity. Read More

View Article and Full-Text PDF

Variation of virulence of five Aspergillus fumigatus isolates in four different infection models.

PLoS One 2021 9;16(7):e0252948. Epub 2021 Jul 9.

Microbiology, Department of Biology, Utrecht University, Utrecht, The Netherlands.

Conidia of Aspergillus fumigatus are inhaled by humans on daily basis. As a consequence, these conidia can cause infections that differ in severity ranging from allergic bronchopulmonary aspergillosis to invasive aspergillosis. In this study we compared virulence of five A. Read More

View Article and Full-Text PDF

Base editing-mediated perturbation of endogenous PKM1/2 splicing facilitates isoform-specific functional analysis in vitro and in vivo.

Cell Prolif 2021 Jul 9:e13096. Epub 2021 Jul 9.

Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China.

Objectives: PKM1 and PKM2, which are generated from the alternative splicing of PKM gene, play important roles in tumourigenesis and embryonic development as rate-limiting enzymes in glycolytic pathway. However, because of the lack of appropriate techniques, the specific functions of the 2 PKM splicing isoforms have not been clarified endogenously yet.

Materials And Methods: In this study, we used CRISPR-based base editors to perturbate the endogenous alternative splicing of PKM by introducing mutations into the splicing junction sites in HCT116 cells and zebrafish embryos. Read More

View Article and Full-Text PDF

Short exposure to glyphosate induces locomotor, craniofacial, and bone disorders in zebrafish (Danio rerio) embryos.

Environ Toxicol Pharmacol 2021 Jul 6;87:103700. Epub 2021 Jul 6.

Centro de Investigación en Alimentación y Desarrollo, A. C. Avenida Sábalo-Cerritos s/n, Mazatlán, Sinaloa 82100, Mexico. Electronic address:

Glyphosate [N-(phosphonomethyl)glycine] is the active ingredient in widely used broad-spectrum herbicides. Even though the toxicity mechanism of this herbicide in vertebrates is poorly understood, evidence suggests that glyphosate is an endocrine disruptor capable of producing morphological anomalies as well as cardiotoxic and neurotoxic effects. We used the zebrafish model to assess the effects of early life glyphosate exposure on the development of cartilage and bone tissues and organismal responses. Read More

View Article and Full-Text PDF

Zebrafish mutants provide insights into Apolipoprotein B functions during embryonic development and pathological conditions.

JCI Insight 2021 Jul 8;6(13). Epub 2021 Jul 8.

Apolipoprotein B (ApoB) is the primary protein of chylomicrons, VLDLs, and LDLs and is essential for their production. Defects in ApoB synthesis and secretion result in several human diseases, including abetalipoproteinemia and familial hypobetalipoproteinemia (FHBL1). In addition, ApoB-related dyslipidemia is linked to nonalcoholic fatty liver disease (NAFLD), a silent pandemic affecting billions globally. Read More

View Article and Full-Text PDF

Is Essential for Primitive Erythropoiesis.

Front Physiol 2021 21;12:685242. Epub 2021 Jun 21.

Molecular Pediatric Hematology Laboratory, Schneider Children's Medical Center of Israel, Petach Tikva, Israel.

Congenital dyserythropoietic anemia type I (CDA I) is an autosomal recessive disease characterized by moderate to severe macrocytic anemia and pathognomonic morphologic abnormalities of the erythroid precursors, including spongy heterochromatin. The disease is mainly caused by mutations in (encoding for Codanin-1). No patients with homozygous null type mutations have been described, and mouse null mutants die during early embryogenesis prior to the initiation of erythropoiesis. Read More

View Article and Full-Text PDF

Single-cell transcriptome analysis of the zebrafish embryonic trunk.

PLoS One 2021 7;16(7):e0254024. Epub 2021 Jul 7.

Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States of America.

During embryonic development, cells differentiate into a variety of distinct cell types and subtypes with diverse transcriptional profiles. To date, transcriptomic signatures of different cell lineages that arise during development have been only partially characterized. Here we used single-cell RNA-seq to perform transcriptomic analysis of over 20,000 cells disaggregated from the trunk region of zebrafish embryos at the 30 hpf stage. Read More

View Article and Full-Text PDF

Neurulation in the posterior region of zebrafish, Danio rerio embryos.

J Morphol 2021 Jul 7. Epub 2021 Jul 7.

Department of Embryology of the Faculty of Biology of St. Petersburg University, St. Petersburg, Russian Federation.

The neural tube of amniotes is formed through different mechanisms that take place in the anterior and posterior regions and involve neural plate folding or mesenchymal condensation followed by its cavitation. Meanwhile, in teleost trunk region, the neural plate forms the neural keel, while the lumen develops later. However, the data on neurulation and other morphogenetic processes in the posterior body region in Teleostei remain fragmentary. Read More

View Article and Full-Text PDF

Enantioselective Toxicity Effects of 2,2',3,5',6-Pentachloro Biphenyl (PCB-95) on Developing Brains in Zebrafish Larvae.

Bull Environ Contam Toxicol 2021 Jul 6. Epub 2021 Jul 6.

Environmental Toxicology Program, Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, SC, USA.

2,2',3,5',6-Pentachlorobiphenyl (PCB-95) is an environmentally relevant, chiral PCB congener that has been shown to act as a developmental neurotoxicant (DNT), targeting the developing brain. However, understanding enantioselective toxic effects for PCB-95 is in its infancy. To investigate these toxic effects, zebrafish embryos were exposed to racemates and enantiomers of PCB-95. Read More

View Article and Full-Text PDF