44,371 results match your criteria zebrafish

Monte Carlo determination of dose coefficients at different developmental stages of zebrafish (Danio rerio) in experimental condition.

J Environ Radioact 2021 Jun 8;237:106667. Epub 2021 Jun 8.

State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, 215123, China; Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions. Electronic address:

The release of liquid effluent of nuclear power into aquatic system increases with the rapid development of nuclear facilities in coastal and inland regions. Aquatic model animals are very important for the study of the radiation hazards to non-human biota in water environment and its extrapolation of dose-effect relationship to human models. However, the study of the radiation dose rate calculation model of the aquatic animal zebrafish is still on the homogeneous isotropic model used for the protection of the environment. Read More

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CD44 loss of function sensitizes AML cells to the BCL-2 inhibitor venetoclax by decreasing CXCL12-driven survival cues.

Blood 2021 Jun 3. Epub 2021 Jun 3.

Karlsruhe Institute for Technology, Eggenstein-Leopoldshafen, Germany.

Acute myeloid leukemia (AML) has a poor prognosis under the current standard of care. In recent years, venetoclax, a BCL-2 inhibitor, was approved to treat patients, ineligible for intensive induction chemotherapy. Complete remission rates with venetoclax-based therapies are, however, hampered by minimal residual disease (MRD) in a proportion of patients, leading to relapse. Read More

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An in vivo reporter for tracking lipid droplet dynamics in transparent zebrafish.

Elife 2021 Jun 11;10. Epub 2021 Jun 11.

Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, United States.

Lipid droplets are lipid storage organelles found in nearly all cell types from adipocytes to cancer cells. Although increasingly implicated in disease, current methods to study lipid droplets in vertebrate models rely on static imaging or the use of fluorescent dyes, limiting investigation of their rapid in vivo dynamics. To address this, we created a lipid droplet transgenic reporter in whole animals and cell culture by fusing tdTOMATO to Perilipin-2 (PLIN2), a lipid droplet structural protein. Read More

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Evaluation of antioxidant, antinflammatory and antityrosinase potential of extracts from different aerial parts of Rhanterium suaveolens from Tunisia.

Chem Biodivers 2021 Jun 11. Epub 2021 Jun 11.

University of Messina: Universita degli Studi di Messina, Chemical Biological Pharmaceutical and Environmental Science, Cda Palatucci, Italia, 98168, Messina, ITALY.

The genus Rhanterium (Asteraceae) is a widely distributed medicinal plant throughout western North Africa and some Rhanterium species are used in folk medicine. The aim of research was to investigate methanolic extracts from different parts (flowers, leaves, and stems) of Tunisian Rhanterium suaveolens as potential sources of bioactive products useful for healthy purposes. In particular, were analyzed the phenolic composition of these extracts and their antioxidant, anti-inflammatory, and anti-tyrosinase properties. Read More

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Construction of a novel asymmetric imidazole-cored AIE probe for ratiometric imaging of endogenous leucine aminopeptidase.

Chem Commun (Camb) 2021 Jun 11. Epub 2021 Jun 11.

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410013, P. R. China. and Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases, Changsha 410078, China.

We report a rational strategy to deliberately construct the first asymmetric tetraarylimidazole-based AIE probe, integrating AIE behavior in synergy with ESIPT character to image endogenous LAP for the first time. It offered good sensitivity and selectivity, and concomitantly, was applied successfully for real-time tracking of LAP in the cisplatin-induced liver injury zebrafish model. Read More

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Occurrence of foamy macrophages during the innate response of zebrafish to trypanosome infections.

Elife 2021 Jun 11;10. Epub 2021 Jun 11.

Cell Biology and Immunology Group, Wageningen University & Research, Wageningen, Netherlands.

A tightly regulated innate immune response to trypanosome infections is critical to strike a balance between parasite control and inflammation-associated pathology. In this study, we make use of the recently established Trypanosoma carassii infection model in larval zebrafish to study the early response of macrophages and neutrophils to trypanosome infections in vivo. We consistently identified high- and low-infected individuals and were able to simultaneously characterize their differential innate response. Read More

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Heterozygous loss-of-function variants significantly expand the phenotypes associated with loss of GDF11.

Genet Med 2021 Jun 10. Epub 2021 Jun 10.

Brigham and Women's Hospital, Boston, MA, USA.

Purpose: Growth differentiation factor 11 (GDF11) is a key signaling protein required for proper development of many organ systems. Only one prior study has associated an inherited GDF11 variant with a dominant human disease in a family with variable craniofacial and vertebral abnormalities. Here, we expand the phenotypic spectrum associated with GDF11 variants and document the nature of the variants. Read More

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Expression profiling and functional characterization of the duplicated Oxr1b gene in zebrafish.

Comp Biochem Physiol Part D Genomics Proteomics 2021 May 26;39:100857. Epub 2021 May 26.

Institute of Three Gorges Ecological Fisheries of Chongqing, College of Fisheries, Southwest University, Chongqing 400715, China; Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University, Chongqing 400715, China. Electronic address:

Oxidation Resistance Gene 1 (OXR1) is a conserved gene family involved in protecting various species against oxidative stress. The zebrafish expresses a pair of OXR1 paralogs (i.e. Read More

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Lipolysis and lipophagy play individual and interactive roles in regulating triacylglycerol and cholesterol homeostasis and mitochondrial form in zebrafish.

Biochim Biophys Acta Mol Cell Biol Lipids 2021 Jun 7:158988. Epub 2021 Jun 7.

LANEH, School of Life Sciences, East China Normal University, Shanghai, China. Electronic address:

Neutral lipases-mediated lipolysis and acid lipases-moderated lipophagy are two main processes for degradation of lipid droplets (LDs). However, the individual and interactive roles of these metabolic pathways are not well known across vertebrates. This study explored the roles of lipolysis and lipophagy from the aspect of neutral and acid lipases in zebrafish. Read More

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In vivo autophagy quantification: Measuring LC3 and P62 puncta in 3D image system from zebrafish larvae.

Abdalla Elbialy

J Cell Biochem 2021 Jun 10. Epub 2021 Jun 10.

Laboratory of Fish Diseases, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt.

Autophagy is a central pathway in maintaining cellular homeostasis through the recycling of damaged proteins and organelles. Detection of LC3 protein levels by immunofluorescence or western blot analysis is one of the most common ways to measure autophagy. For quantitative autophagy analysis, LC3 western blot analysis is commonly used, whereas immunostaining is used for qualitative autophagy analysis. Read More

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Pyrene-Based AIEE Active Nanoprobe for Zn and Tyrosine Detection Demonstrated by DFT, Bioimaging, and Organic Thin-Film Transistor.

ACS Appl Mater Interfaces 2021 Jun 10. Epub 2021 Jun 10.

Department of Applied Chemistry, National Yang Ming Chiao Tung University (formerly National Chiao Tung University), Hsinchu 300, Taiwan.

The development of aggregation-induced emission enhancement (AIEE) active nanoprobes without any synthetic complication for solution-state and organic thin-film transistor (OTFT)-based sensory applications is still a challenging task. In this study, the novel pyrene-incorporated Schiff base (5-phenyl-4-((pyren-1-ylmethylene)amino)-4-1,2,4-triazole-3-thiol; ) with an AIEE property was synthesized via a one-pot reaction and was reported for detecting Zn and tyrosine in the solution state and OTFT. In the AIEE studies of (in CHCN) at various water fractions (: 0-97. Read More

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Fmrp regulates oligodendrocyte lineage cell specification and differentiation.

Glia 2021 Jun 10. Epub 2021 Jun 10.

Department of Pediatrics, Section of Developmental Biology, University of Colorado School of Medicine, Children's Hospital Colorado, Aurora, Colorado, USA.

Neurodevelopment requires the precise integration of a wide variety of neuronal and glial cell types. During early embryonic development, motor neurons and then oligodendrocyte precursor cells (OPCs) are specified from neural progenitors residing in the periventricular pMN progenitor domain of the spinal cord. Following gliogenesis, OPCs can differentiate as oligodendrocytes (OLs)-the myelinating glial cells of the central nervous system-or remain as OPCs. Read More

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A systematic review to compare chemical hazard predictions of the zebrafish embryotoxicity test with mammalian prenatal developmental toxicity.

Toxicol Sci 2021 Jun 9. Epub 2021 Jun 9.

Evidence-Based Toxicology Collaboration (EBTC), Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, 21205.

Originally developed to inform the acute toxicity of chemicals on fish, the zebrafish embryotoxicity test (ZET) has also been proposed for assessing the prenatal developmental toxicity of chemicals, potentially replacing mammalian studies. While extensively evaluated in primary studies, a comprehensive review summarizing the available evidence for the ZET's capacity is lacking. Therefore, we conducted a systematic review of how well the presence or absence of exposure-related findings in the ZET predict prenatal development toxicity in studies with rats and rabbits. Read More

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Exercise, programmed cell death and exhaustion of cardiomyocyte proliferation in aging zebrafish.

Dis Model Mech 2021 Jun 10. Epub 2021 Jun 10.

Biosciences Institute, Faculty of Medicine, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne, NE1 3BZ.

As the human heart ages, the myocardium undergoes fibrotic remodelling, there is declining cardiovascular performance and eventual heart failure. It is suggested that exercise is an important intervention to ameliorate these changes. In this study we establish zebrafish as a laboratory model to understand how aging and exercise affect cardiomyocyte turnover. Read More

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N-Methyladenine in Eukaryotic DNA: Tissue Distribution, Early Embryo Development, and Neuronal Toxicity.

Front Genet 2021 24;12:657171. Epub 2021 May 24.

Immune Endocrine Epigenetics Research Group, Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg.

DNA methylation is one of the most important epigenetic modifications and is closely related with several biological processes such as regulation of gene transcription and the development of non-malignant diseases. The prevailing dogma states that DNA methylation in eukaryotes occurs essentially through 5-methylcytosine (5mC) but recently adenine methylation was also found to be present in eukaryotes. In mouse embryonic stem cells, 6-methyladenine (6mA) was associated with the repression and silencing of genes, particularly in the X-chromosome, known to play an important role in cell fate determination. Read More

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Spatiotemporal imaging and pharmacokinetics of fluorescent compounds in zebrafish eleuthero-embryos after different routes of administration.

Sci Rep 2021 Jun 9;11(1):12229. Epub 2021 Jun 9.

Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, Belgium.

Zebrafish (Danio rerio) is increasingly used to assess the pharmacological activity and toxicity of compounds. The spatiotemporal distribution of seven fluorescent alkyne compounds was examined during 48 h after immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and yolk sac (IY) of 3 dpf zebrafish eleuthero-embryos. By modelling the fluorescence of whole-body contours present in fluorescence images, the main pharmacokinetic (PK) parameter values of the compounds were determined. Read More

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Pyridaben induced cardiotoxicity during the looping stages of zebrafish (Danio rerio) embryos.

Aquat Toxicol 2021 May 19;237:105870. Epub 2021 May 19.

Ganzhou Key Laboratory for Drug Screening and Discovery, School of Geography and Environmental Engineering, Gannan Normal University, Ganzhou 341000, Jiangxi, China. Electronic address:

Pyridaben is a widely used acaricide in agriculture and reaches a high concentration (97 μg/L) in paddy water for a short time when pyridaben was applied to rice. However, its toxicity to aquatic organisms is still poorly understood. Therefore, we assessed the pyridaben cardiotoxicity to aquatic organisms using the zebrafish (Danio rerio) model. Read More

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Integrin intra-heterodimer affinity inversely correlates with integrin activatability.

Cell Rep 2021 Jun;35(10):109230

Department of Molecular, Cellular and Developmental Biology, Yale University, 260 Whitney Avenue, New Haven, CT 06520, USA. Electronic address:

Integrins are heterodimeric cell surface receptors composed of an α and β subunit that mediate cell adhesion to extracellular matrix proteins such as fibronectin. We previously studied integrin α5β1 activation during zebrafish somitogenesis, and in the present study, we characterize the integrin αV fibronectin receptors. Integrins are activated via a conformational change, and we perform single-molecule biophysical measurements of both integrin activation via fluorescence resonance energy transfer (FRET)-fluorescence lifetime imaging microscopy (FLIM) and integrin intra-heterodimer stability via fluorescence cross-correlation spectroscopy (FCCS) in living embryos. Read More

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Stage Dependent Enantioselective Metabolism of Bifenthrin in Embryos of Zebrafish () and Japanese Medaka ().

Environ Sci Technol 2021 Jun 9. Epub 2021 Jun 9.

Department of Environmental Sciences, University of California, Riverside, California 92521, United States.

Bifenthrin (BF) is a widely used pyrethroid that has been frequently detected in surface waters. Previous studies indicated that BF had antiestrogenic activity in zebrafish embryos but estrogenic activity in posthatch fish. To determine whether age-related differences in metabolism contribute to the endocrine effects in developing fish, embryos from zebrafish and Japanese medaka were exposed to BF before and after liver development. Read More

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Use of melatonin as an inhibitor of apoptotic process for cryopreservation of zebrafish (Danio rerio) embryos.

Braz J Biol 2021 2;82:e241081. Epub 2021 Jun 2.

Universidade Estadual de Maringá - UEM, Departamento de Zootecnia, Maringá, PR, Brasil.

This study investigated the use of melatonin to arrest the effects of apoptosis in vitrified zebrafish (D. rerio) embryos. Dechorionated embryos at 22-24 somite-stage were divided (n = 60/treatment) into a non-vitrified (Control Group, 0 M melatonin) and vitrified treatments with 0 M (T1), 1 µM (T2) and 1 mM of melatonin (T3). Read More

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Planar polarization of cilia in the zebrafish floor-plate involves Par3-mediated posterior localization of highly motile basal bodies.

Development 2021 Jun 9. Epub 2021 Jun 9.

Sorbonne Université, CNRS UMR7622, INSERM U1156, Institut de Biologie Paris Seine (IBPS) - Developmental Biology Unit, 75005, Paris, France.

Epithelial cilia, whether motile or primary, often display an off-centered planar localization within the apical cell surface. This form of planar cell polarity (PCP) involves the asymmetric positioning of the ciliary basal body (BB). Using the mono-ciliated epithelium of the embryonic zebrafish floor-plate, we investigated the dynamics and mechanisms of BB polarization by live-imaging. Read More

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Inhibition of Lipopolysaccharide-Induced Inflammatory and Oxidative Responses by -cinnamaldehyde in C2C12 Myoblasts.

Int J Med Sci 2021 23;18(12):2480-2492. Epub 2021 Apr 23.

Anti-Aging Research Center, Dong-eui University, Busan 47340, Republic of Korea.

-cinnamaldehyde (tCA), a bioactive component found in , has been reported to exhibit anti-inflammatory and antioxidant effects, but its efficacy in muscle cells has yet to be found. In this study, we investigated the inhibitory effect of tCA on inflammatory and oxidative stress induced by lipopolysaccharide (LPS) in C2C12 mouse skeletal myoblasts. To investigate the anti-inflammatory and antioxidant effects of tCA in LPS-treated C2C12 cells, we measured the levels of pro-inflammatory mediator, cytokines, and reactive oxygen species (ROS). Read More

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Npas4a expression in the teleost forebrain is associated with stress coping style differences in fear learning.

Sci Rep 2021 Jun 8;11(1):12074. Epub 2021 Jun 8.

Department of Biology, University of Nebraska at Omaha, Omaha, USA.

Learning to anticipate potentially dangerous contexts is an adaptive behavioral response to coping with stressors. An animal's stress coping style (e.g. Read More

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Early inflammation dysregulates neuronal circuit formation via upregulation of IL-1β.

J Neurosci 2021 Jun 8. Epub 2021 Jun 8.

Department of Neurobiology and Neurosurgery, Montreal Neurological Institute, McGill University, 3801 University Street, Montreal, Canada H3A 2B4

Neuro-immune interaction during development is strongly implicated in the pathogenesis of neurodevelopmental disorders, but the mechanisms that cause neuronal circuit dysregulation are not well understood. We performed imaging of the developing retinotectal system in the larval zebrafish to characterize the effects of immune system activation on refinement of an archetypal sensory processing circuit. Acute inflammatory insult induced hyper-dynamic remodeling of developing retinal axons in larval fish and increased axon arbor elaboration over days. Read More

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Oxytetracycline induces anxiety-like behavior in adult zebrafish.

Toxicol Appl Pharmacol 2021 Jun 5:115616. Epub 2021 Jun 5.

Programa de Pós-Graduação em Biologia Celular e Molecular, Escola de Ciências da Saúde e da Vida, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Medicina e Ciências da Saúde, Escola de Medicina, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address:

Oxytetracycline (OTC) is one of the broad-spectrum antibiotics widely used for the treatment of fish-farm infection. Considering that behavior is directly related to reproduction, individual fitness, and survival, it is important to evaluate the impact of antibiotics on the behavioral repertoire in fish. Zebrafish (Danio rerio) presents a well-described behavioral repertoire to reliably demonstrate complex responses to chemical compound exposure. Read More

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The tectum/superior colliculus as the vertebrate solution for spatial sensory integration and action.

Curr Biol 2021 Jun;31(11):R741-R762

The Queensland Brain Institute, The University of Queensland, St Lucia, QLD 4072, Australia. Electronic address:

The superior colliculus, or tectum in the case of non-mammalian vertebrates, is a part of the brain that registers events in the surrounding space, often through vision and hearing, but also through electrosensation, infrared detection, and other sensory modalities in diverse vertebrate lineages. This information is used to form maps of the surrounding space and the positions of different salient stimuli in relation to the individual. The sensory maps are arranged in layers with visual input in the uppermost layer, other senses in deeper positions, and a spatially aligned motor map in the deepest layer. Read More

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De novo and bi-allelic variants in AP1G1 cause neurodevelopmental disorder with developmental delay, intellectual disability, and epilepsy.

Am J Hum Genet 2021 Jun 3. Epub 2021 Jun 3.

Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.

Adaptor protein (AP) complexes mediate selective intracellular vesicular trafficking and polarized localization of somatodendritic proteins in neurons. Disease-causing alleles of various subunits of AP complexes have been implicated in several heritable human disorders, including intellectual disabilities (IDs). Here, we report two bi-allelic (c. Read More

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Natural infection of covert mortality nodavirus affects Zebrafish (Danio rerio).

J Fish Dis 2021 Jun 8. Epub 2021 Jun 8.

Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.

Covert mortality nodavirus (CMNV), a novel aquatic pathogen, causes viral covert mortality disease (VCMD) in shrimps and also known to infect farmed marine fish. To date, there has no report regarding the ability of this virus to infect freshwater fish. In this study, we screened and discovered CMNV-positive freshwater zebrafish individuals by reverse transcription-nested PCR (RT-nPCR). Read More

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Colored-Light Preference in Zebrafish ().

Zebrafish 2021 Jun 8. Epub 2021 Jun 8.

Department of Psychology and University of Toronto Mississauga, Mississauga, Canada.

Over the past decade, the zebrafish has been increasingly employed in biomedical neuroscience research due to its numerous evolutionarily conserved features with mammals. Its simple brain and the several molecular tools available for this species make the zebrafish an appealing model to study mechanisms of complex brain functions, including learning and memory. Most learning paradigms developed for the zebrafish have employed visual stimuli as the associative cue. Read More

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Exogenous Expression of Facilitates the Development of Somatic Cell Nuclear Transfer Embryos in Zebrafish at the Early Stage.

Cell Reprogram 2021 Jun 8. Epub 2021 Jun 8.

College of Life Sciences, Peking University, Beijing, P.R. China.

Enucleated oocytes can reprogram differentiated nuclei to totipotency after somatic cell nuclear transfer (SCNT), which is valuable in understanding nuclear reprogramming and generating genetically modified animals. To date, reprogramming efficiency is low and the development of SCNT embryos is not going as well as anticipated. To further disclose the reprogramming mechanisms during SCNT zebrafish embryo development, we examined the expression patterns of transcription regulation factors and regulated them by mRNA and morpholino microinjection. Read More

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