46,947 results match your criteria yolk proteins

Elevated temperatures accelerate the formation of toxic amyloid fibrils of hen egg-white lysozyme.

Vet Med Sci 2021 May 12. Epub 2021 May 12.

School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, P.R. China.

The formation of amyloid fibrils is critical for neurodegenerative diseases. Some physiochemical conditions can promote the conversion of proteins from soluble globular shapes into insoluble well-organized amyloid fibrils. The aim of this study was to investigate the effect of temperatures on amyloid fibrils formation in vitro using the protein model of hen egg-white lysozyme (HEWL). Read More

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Re-thinking yellow fever vaccines: fighting old foes with new generation vaccines.

Hum Vaccin Immunother 2021 May 11:1-9. Epub 2021 May 11.

The Jenner Institute, Nuffield Department of Medicine, University of Oxford. The Henry Wellcome Building for Molecular Physiology, Oxford, UK.

Despite the existence of a highly efficient yellow fever vaccine, yellow fever reemergence throughout Africa and the Americas has put 900 million people in 47 countries at risk of contracting the disease. Although the vaccine has been key to controlling yellow fever epidemics, its live-attenuated nature comes with a range of contraindications that prompts advising against its administration to pregnant and lactating women, immunocompromised individuals, and those with hypersensitivity to chicken egg proteins. Additionally, large outbreaks have highlighted problems with insufficient vaccine supply, whereby manufacturers rely on slow traditional manufacturing processes that prevent them from ramping up production. Read More

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Modeling and Experimental Validation of Algorithms for Maximum Quantity of Protein to be Immobilized on Solid Supports by Electrostatic Adsorption in the Strategy of Rational Design of Immobilized Derivatives.

Protein J 2021 May 10. Epub 2021 May 10.

Centro de Estudio de Proteínas, Facultad de Biología, Universidad de La Habana, Calle 25, #455, e/ J e I, Vedado, 10400, Havana, Cuba.

Protein immobilization by electrostatic adsorption to a support could represent a good option. On the other hand, lysozyme (EC 3.2. Read More

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X-Ray Photoelectron Spectroscopy on Microbial Cell Surfaces: A Forgotten Method for the Characterization of Microorganisms Encapsulated With Surface-Engineered Shells.

Front Chem 2021 22;9:666159. Epub 2021 Apr 22.

University of Groningen and University Medical Center of Groningen, Department of Biomedical Engineering, Groningen, Netherlands.

Encapsulation of single microbial cells by surface-engineered shells has great potential for the protection of yeasts and bacteria against harsh environmental conditions, such as elevated temperatures, UV light, extreme pH values, and antimicrobials. Encapsulation with functionalized shells can also alter the surface characteristics of cells in a way that can make them more suitable to perform their function in complex environments, including bio-reactors, bio-fuel production, biosensors, and the human body. Surface-engineered shells bear as an advantage above genetically-engineered microorganisms that the protection and functionalization added are temporary and disappear upon microbial growth, ultimately breaking a shell. Read More

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SSX C-Terminus is a Useful Diagnostic Biomarker for Spermatocytic Tumour.

Histopathology 2021 May 7. Epub 2021 May 7.

Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, United States.

Aims: Spermatocytic tumour (ST) is a rare testicular germ cell neoplasm that can be challenging to diagnose and for which there are few confirmatory biomarkers. Like normal spermatogonia, STs are known to express SSX proteins. Recently, a novel SSX antibody directed against the C-terminus (SSX_CT) of SSX1, SSX2 and SSX4, has emerged as a reliable biomarker for these SSX proteins and synovial sarcoma. Read More

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Effects of hatching enzymes on egg envelope digestion in the male-brooding seahorse.

Mol Reprod Dev 2021 May 7. Epub 2021 May 7.

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), South China Sea Institute of Oceanology, Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, China.

In the present study, we aimed to evaluate the effects of hatching enzymes on the egg envelope digestion during the hatching period in the male brooding seahorse. The complementary DNAs encoding two hatching-enzyme genes, high choriolytic enzyme (HCE) and low choriolytic enzyme (LCE), were cloned and functionally characterized from the lined seahorse (Hippocampus erectus). The genomic-synteny analysis confirmed that teleosts shared LCE gene synteny. Read More

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Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts.

Mol Biol Cell 2021 May 6:mbcE20070461. Epub 2021 May 6.

Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA, 19104.

Mitotic kinetochores assemble via the hierarchical recruitment of numerous cytosolic components to the centromere region of each chromosome. However, how these orderly and localized interactions are achieved without spurious macromolecular assemblies forming from soluble kinetochore components in the cell cytosol remains poorly understood. We developed assembly assays to monitor the recruitment of GFP-tagged recombinant proteins and native proteins from human cell extracts to inner kinetochore components immobilized on microbeads. Read More

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Monitoring the carryover of egg proteins in pasta making to support allergen risk management.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2021 May 6:1-9. Epub 2021 May 6.

Department of Food, Environmental and Nutritional Sciences (Defens), University of Milan, Milan, Italy.

Egg proteins are among the major food allergens. Very often, the same pasta-making plants are used for industrial production of egg-based pasta (EBP) and semolina-only pasta (SP), so that residual egg proteins may be present in SP. This calls for defining the amount of semolina pasta that should be discarded when switching production lines. Read More

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Improving gelling properties of diluted whole hen eggs with sodium chloride and sodium tripolyphosphate: Study on intermolecular forces, water state and microstructure.

Food Chem 2021 Apr 20;358:129823. Epub 2021 Apr 20.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:

Individual and synergistic effects of sodium chloride (NaCl) and sodium tripolyphosphate (STPP) on the physicochemical and gelling properties of highly diluted liquid whole eggs were studied. Results showed that NaCl and STPP acted differently whereby NaCl addition increased the surface hydrophobicity of the egg proteins and STPP addition increased the protein solubility and the negative surface charge. When combined together, these changes led to a significant increase in a number of intermolecular forces after heat treatment, including hydrophobic interactions, disulfide bonds, and hydrogen bonds, contributing to the best structure and texture of the whole egg gels enriched with binary salts. Read More

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Prohibitin depletion extends lifespan of a TORC2/SGK-1 mutant through autophagy and the mitochondrial UPR.

Aging Cell 2021 May 3:e13359. Epub 2021 May 3.

Andalusian Centre for Developmental Biology, Consejo Superior de Investigaciones Científicas/Junta de Andalucía/Universidad Pablo de Olavide, Seville, Spain.

Mitochondrial prohibitins (PHB) are highly conserved proteins with a peculiar effect on lifespan. While PHB depletion shortens lifespan of wild-type animals, it enhances longevity of a plethora of metabolically compromised mutants, including target of rapamycin complex 2 (TORC2) mutants sgk-1 and rict-1. Here, we show that sgk-1 mutants have impaired mitochondrial homeostasis, lipogenesis and yolk formation, plausibly due to alterations in membrane lipid and sterol homeostasis. Read More

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Serum proteomic profiling in patients with advanced Schistosoma japonicum-induced hepatic fibrosis.

Parasit Vectors 2021 May 1;14(1):232. Epub 2021 May 1.

Institute of Hepatology, The Affiliated Hospital of Jiaxing University, Jiaxing, 314001, Zhejiang, People's Republic of China.

Background: Schistosoma japonicum is a parasitic flatworm that is the aetiological agent of human schistosomiasis, an important cause of hepatic fibrosis. Schistosomiasis-induced hepatic fibrosis is a consequence of the highly fibrogenic nature of egg-induced granulomatous lesions, which are the main pathogenic features of schistosomiasis. Although global awareness of the association between schistosomiasis-induced hepatic fibrosis and S. Read More

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RNA sequencing-based analysis of the magnum tissues revealed the novel genes and biological pathways involved in the egg-white formation in the laying hen.

BMC Genomics 2021 May 1;22(1):318. Epub 2021 May 1.

Department of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, HI, 96822, Honolulu, USA.

Background: The mechanism of egg formation in the oviduct of laying hens is tightly controlled; each segment of the oviduct contributes a unique component of the egg. Several genes/proteins are involved in the synthesis of a completely healthy egg. This implies a time- and tissue-specific expression of genes and proteins in the different oviductal segments. Read More

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Three-dimensional structures of avian beta-microseminoproteins: insight from the chicken egg-specific beta-microseminoprotein 3 paralog.

FEBS Open Bio 2021 May 1. Epub 2021 May 1.

INRAE, Université de Tours, BOA, Nouzilly, France.

Beta-microseminoproteins (MSMBs) are small disulfide-rich proteins that are conserved among vertebrates. These proteins exhibit diverse biological activities and were mainly reported to play a role in male fertility, immunity, and embryogenesis. In this work, we focused on the chicken MSMB3 protein that was previously depicted as an egg antibacterial protein. Read More

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Proteomic Analysis Identifies Potential Markers for Chicken Primary Follicle Development.

Animals (Basel) 2021 Apr 13;11(4). Epub 2021 Apr 13.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530000, China.

Follicles' development in chicken imparts a major impact on egg production. To enhance the egg-laying efficiency, comprehensive knowledge of different phases of follicular development is a prerequisite. Therefore, we used the tandem mass tag (TMT) based proteomic approach to find the genes involved in the primary follicular development of chicken. Read More

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In Vitro Characterization of Neutralizing Hen Antibodies to A16.

Int J Mol Sci 2021 Apr 16;22(8). Epub 2021 Apr 16.

Ph.D. Program in Medical Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 110301, Taiwan.

A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). Children aged <5 years are the most affected by CA16 HFMD globally. Although clinical symptoms of CA16 infections are usually mild, severe complications, such as aseptic meningitis or even death, have been recorded. Read More

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A Comprehensive Study Monitoring the Venom Composition and the Effects of the Venom of the Rare Ethiopian Endemic Snake Species .

Toxins (Basel) 2021 04 22;13(5). Epub 2021 Apr 22.

Department of Pharmacology and Toxicology, The University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia.

The Ethiopian endemic snake of the species recognized for its colorful patterns, might be more interesting as we look deeper into the venom activity. We assayed the effects of venoms from the most widespread venomous African and closely related species using The Hen's Egg Test-Chorioallantoic membrane test (HET-CAM) and Chicken embryotoxicity screening test (CHEST), acetylcholinesterase (AChE) analysis, cytotoxicity assay performed on cell lines and protein analysis of selected venoms. Our results indicated that venom contains vasoactive compounds that have a direct effect on blood vessels. Read More

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Thermodynamic Evaluation of the Interactions between Anticancer Pt(II) Complexes and Model Proteins.

Molecules 2021 Apr 19;26(8). Epub 2021 Apr 19.

Department of Chemistry and Industrial Chemistry, University of Pisa, Via G. Moruzzi 13, 56124 Pisa, Italy.

In this work, we have analysed the binding of the Pt(II) complexes ([PtCl(4'-phenyl-2,2':6',2″-terpyridine)](CFSO) (), [PtI(4'-phenyl-2,2':6',2″-terpyridine)](CFSO) () and [PtCl(1,3-di(2-pyridyl)benzene) ()] with selected model proteins (hen egg-white lysozyme, HEWL, and ribonuclease A, RNase A). Platinum coordination compounds are intensively studied to develop improved anticancer agents. In this regard, a critical issue is the possible role of Pt-protein interactions in their mechanisms of action. Read More

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Phosphate Elimination in Emulsified Meat Products: Impact of Protein-Based Ingredients on Quality Characteristics.

Foods 2021 Apr 17;10(4). Epub 2021 Apr 17.

Research Group for Technology and Quality of Animal Products, Department of Microbial and Molecular Systems, Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven Ghent Technology Campus, Gebroeders De Smetstraat 1, B-9000 Gent, Belgium.

The addition of phosphates to meat products improves the emulsifying and gelling properties of meat proteins, in turn enhancing overall product quality. The current market trend towards additive-free products and the health issues related to phosphate challenge the industry to develop phosphate-free meat products. The aim of this study was to evaluate the potential of seven protein-based ingredients (pea, blood plasma, gelatin, soy, whey, egg, and potato) to remediate quality losses of emulsified meat products (cooked sausages) upon phosphate elimination. Read More

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Identification of Low Molecular Weight Proteins and Peptides from Worm, Egg and Infected Mouse Sera.

Biomolecules 2021 Apr 11;11(4). Epub 2021 Apr 11.

Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.

is found in the lower Mekong river region and causes schistosomiasis. Low sensitivity of diagnosis and development of drug resistance are problems to eliminate this disease. To develop novel therapies and diagnostics for , the basic molecular biology of this pathogen needs to be explored. Read More

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Influence of pH, Temperature and Protease Inhibitors on Kinetics and Mechanism of Thermally Induced Aggregation of Potato Proteins.

Foods 2021 Apr 8;10(4). Epub 2021 Apr 8.

Food and Bioprocess Engineering, TUM School of Life Sciences, Technical University of Munich, Weihenstephaner Berg 1, 85354 Freising, Germany.

Understanding aggregation in food protein systems is essential to control processes ranging from the stabilization of colloidal dispersions to the formation of macroscopic gels. Patatin rich potato protein isolates (PPI) have promising techno-functionality as alternatives to established proteins from egg white or milk. In this work, the influence of pH and temperature on the kinetics of PPI denaturation and aggregation was investigated as an option for targeted functionalization. Read More

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Knock out of specific maternal vitellogenins in zebrafish (Danio rerio) evokes vital changes in egg proteomic profiles that resemble the phenotype of poor quality eggs.

BMC Genomics 2021 Apr 28;22(1):308. Epub 2021 Apr 28.

INRAE, LPGP, 35000, Rennes, France.

Background: We previously reported the results of CRISPR/Cas9 knock-out (KO) of type-I and type-III vitellogenins (Vtgs) in zebrafish, which provided the first experimental evidence on essentiality and disparate functioning of Vtgs at different stages during early development. However, the specific contributions of different types of Vtg to major cellular processes remained to be investigated. The present study employed liquid chromatography and tandem mass spectrometry (LC-MS/MS) to meet this deficit. Read More

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Minimal epitope for Mannitou IgM on paucimannose-carrying glycoproteins.

Glycobiology 2021 Apr 28. Epub 2021 Apr 28.

Unité de Glycobiologie Structurale et Fonctionnelle, UMR 8576 du CNRS et Université de Lille, 50 Avenue Halley, 59650 Villeneuve d'Ascq, France.

Paucimannosidic glycans are restricted to the core structure [Man1-3GlcNAc2Fuc0-1] of N-glycans and are rarely found in mammalian tissues. Yet, especially [Man2-3GlcNAc2Fuc1] have been found significantly upregulated in tumors, including in colorectal and liver cancer. Mannitou IgM is a murine monoclonal antibody that was previously shown to recognise Man3GlcNAc2 with an almost exclusive selectivity. Read More

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Effects of salt and heat treatment on the physicochemical properties, microstructure, secondary structure, and simulated in vitro gastrointestinal digestion of duck egg white.

J Sci Food Agric 2021 Apr 27. Epub 2021 Apr 27.

Jiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang, China.

Background: The texture and structure of the duck egg white (DEW) gel under salt and heat treatment are crucial to its digestibility. Specifically, the structural changes of food protein gels have been recognized for their potential to regulate in vitro digestion. In this study, the effects of gel characteristics and simulated in vitro gastrointestinal digestion of DEW under combined salt and heat treatment were investigated. Read More

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E93 confers steroid hormone responsiveness of digestive enzymes to promote blood meal digestion in the midgut of the mosquito Aedes aegypti.

Insect Biochem Mol Biol 2021 Apr 24;134:103580. Epub 2021 Apr 24.

Department of Entomology, University of California, Riverside, CA, 92521, USA; Institute of Integrative Genome Biology, University of California, Riverside, CA, 92521, USA. Electronic address:

Anautogenous female mosquitoes obtain the nutrients needed for egg development from vertebrate blood, and consequently they transmit numerous pathogens of devastating human diseases. Digestion of blood proteins into amino acids that are used for energy production, egg maturation and replenishment of maternal reserves is an essential part of the female mosquito reproductive cycle. However, the regulatory mechanisms underlying this process remain largely unknown. Read More

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Knock out of a major vitellogenin receptor gene with eight ligand binding repeats in medaka (Oryzias latipes) using the CRISPR/Cas9 system.

Comp Biochem Physiol A Mol Integr Physiol 2021 Apr 23;257:110967. Epub 2021 Apr 23.

Division of Marine Life Sciences, Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato, Hakodate, Hokkaido 041-8611, Japan. Electronic address:

Recent studies of vitellogenesis engendered a novel model of teleost yolk formation in which multiple yolk precursors, vitellogenins (Vtgs), and their receptors (Vtgrs) interact to ensure proper yolk composition for embryonic development and larval growth. As a step toward verification of this concept, we examined the role of one candidate Vtgr, termed low-density lipoprotein receptor relative with eight ligand-binding repeat (Lr8), in the medaka, a representative teleost and established laboratory model. A homozygous lr8 knock out (lr8-KO) medaka was produced to perform reverse-genetic functional analyses. Read More

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Receptor-mediated yolk uptake is required for oskar mRNA localization and cortical anchorage of germ plasm components in the Drosophila oocyte.

PLoS Biol 2021 Apr 23;19(4):e3001183. Epub 2021 Apr 23.

Department of Germline Development, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.

The Drosophila germ plasm is responsible for germ cell formation. Its assembly begins with localization of oskar mRNA to the posterior pole of the oocyte. The oskar translation produces 2 isoforms with distinct functions: short Oskar recruits germ plasm components, whereas long Oskar remodels actin to anchor the components to the cortex. Read More

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Interfacial interactions between protective, surface-engineered shells and encapsulated bacteria with different cell surface composition.

Nanoscale 2021 Apr 7;13(15):7220-7233. Epub 2021 Apr 7.

University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.

Surface-engineered encapsulation is a non-genetic method to protect living organisms against harsh environmental conditions. Different cell encapsulation methods exist, yielding shells with different interfacial-interactions with encapsulated, bacterial surfaces. However, the impact of interfacial-interactions on the protection offered by different shells is unclear and can vary for bacteria with different surface composition. Read More

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Taurine suppresses liquid-liquid phase separation of lysozyme protein.

Amino Acids 2021 Apr 21. Epub 2021 Apr 21.

Department of Biosciences and Biotechnology, Fukui Prefectural University, 4-1-1 Matsuokakenjojima, Eiheiji-cho, Yoshida-gun, Fukui, 910-1195, Japan.

Taurine is a compatible osmolyte that confers stability to proteins. Recent studies have revealed that liquid-liquid phase separation (LLPS) of proteins underlie the formation of membraneless organelles in cells. In the present study, we evaluated the role of taurine on LLPS of hen egg lysozyme. Read More

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Protective effects of Atractylenolide III on inflammation and oxidative stress in ovalbumin-induced asthma mice and its possible mechanisms.

Gen Physiol Biophys 2021 Mar;40(2):137-146

Orthopedics and Integration Medical Department, Hunan Chest Hospital, Changsha, Hunan, China.

Asthma is a complex disorder characterized by chronic inflammation of the airways. We aimed to investigate the role of Atractylenolide III (ATL III) in ovalbumin (OVA)-induced mouse asthma. Asthma was induced to BALB/c mice by sensitization with intraperitoneal injection of OVA, followed by treatment with ATL III. Read More

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Septate junction proteins are required for egg elongation and border cell migration during oogenesis in Drosophila.

G3 (Bethesda) 2021 Apr 19. Epub 2021 Apr 19.

Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, USA.

Protein components of the invertebrate occluding junction-known as the septate junction (SJ) - are required for morphogenetic developmental events during embryogenesis in Drosophila melanogaster. In order to determine whether SJ proteins are similarly required for morphogenesis during other developmental stages, we investigated the localization and requirement of four representative SJ proteins during oogenesis: Contactin, Macroglobulin complement-related, Neurexin IV, and Coracle. A number of morphogenetic processes occur during oogenesis, including egg elongation, formation of dorsal appendages, and border cell migration. Read More

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