28,586 results match your criteria yeast functional

Waste streams in onion production: Bioactive compounds, quercetin and use of antimicrobial and antioxidative properties.

Waste Manag 2021 Apr 7;126:476-486. Epub 2021 Apr 7.

Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia; The Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins (CipKeBiP), Jamova 39, SI-1000, Ljubljana, Slovenia. Electronic address:

Onion production generates abundant waste with high contents of bioactive compounds. These might have several beneficial functional properties for fortification of foods. To understand the variety and potential for further use, we examined various parts of the plants (edible/inedible waste/outer skin of onion), as well as extraction in water/ethanol and by shaking/sonication. Read More

View Article and Full-Text PDF

Cryo-EM structure and kinetics reveal electron transfer by 2D diffusion of cytochrome in the yeast III-IV respiratory supercomplex.

Proc Natl Acad Sci U S A 2021 Mar;118(11)

Department of Biochemistry and Biophysics, The Arrhenius Laboratories for Natural Sciences, Stockholm University, SE-106 91 Stockholm, Sweden;

Energy conversion in aerobic organisms involves an electron current from low-potential donors, such as NADH and succinate, to dioxygen through the membrane-bound respiratory chain. Electron transfer is coupled to transmembrane proton transport, which maintains the electrochemical proton gradient used to produce ATP and drive other cellular processes. Electrons are transferred from respiratory complexes III to IV (CIII and CIV) by water-soluble cytochrome (cyt. Read More

View Article and Full-Text PDF

A functional screen identifies transcriptional networks that regulate HIV-1 and HIV-2.

Proc Natl Acad Sci U S A 2021 Mar;118(11)

Department of Microbiology, Boston University School of Medicine, Boston, MA 02118;

The molecular networks involved in the regulation of HIV replication, transcription, and latency remain incompletely defined. To expand our understanding of these networks, we performed an unbiased high-throughput yeast one-hybrid screen, which identified 42 human transcription factors and 85 total protein-DNA interactions with HIV-1 and HIV-2 long terminal repeats. We investigated a subset of these transcription factors for transcriptional activity in cell-based models of infection. Read More

View Article and Full-Text PDF

Molecular mechanisms and highly functional development for stress tolerance of the yeast Saccharomyces cerevisiae.

Hiroshi Takagi

Biosci Biotechnol Biochem 2021 Feb 12. Epub 2021 Feb 12.

Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Nara, Japan.

In response to environmental stress, microorganisms adapt to drastic changes while exerting cellular functions by controlling gene expression, metabolic pathways, enzyme activities, and protein-protein interactions. Microbial cells that undergo a fermentation process are subjected to stresses, such as high temperature, freezing, drying, changes in pH and osmotic pressure, and organic solvents. Combinations of these stresses that continue over long terms often inhibit cells' growth and lead to their death, markedly limiting the useful functions of microorganisms (eg their fermentation ability). Read More

View Article and Full-Text PDF
February 2021

In-frame deletion in canine PITRM1 is associated with a severe early-onset epilepsy, mitochondrial dysfunction and neurodegeneration.

Hum Genet 2021 Apr 9. Epub 2021 Apr 9.

Department of Medical and Clinical Genetics, University of Helsinki, Helsinki, Finland.

We investigated the clinical, genetic, and pathological characteristics of a previously unknown severe juvenile brain disorder in several litters of Parson Russel Terriers. The disease started with epileptic seizures at 6-12 weeks of age and progressed rapidly to status epilepticus and death or euthanasia. Histopathological changes at autopsy were restricted to the brain. Read More

View Article and Full-Text PDF

OPDAT1, a plastid envelope protein involved in 12-oxo-phytodienoic acid export for jasmonic acid biosynthesis in Populus.

Tree Physiol 2021 Apr 9. Epub 2021 Apr 9.

Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing 400715, China.

Twelve-oxo-phytodienoic acid (OPDA), the cyclopentenone precursor of jasmonic acid (JA), is required for the wounding response of plants. OPDA is derived from plastid-localized α-linolenic acid (α-LeA; 18:3) via the octadecanoid pathway, and is further exported from plastids to the cytosol for JA biosynthesis. However, the mechanism of OPDA transport from plastids has yet to be elucidated. Read More

View Article and Full-Text PDF

The ecology of wine fermentation: a model for the study of complex microbial ecosystems.

Appl Microbiol Biotechnol 2021 Apr 9. Epub 2021 Apr 9.

Department of Viticulture and Oenology, South African Grape and Wine Research Institute, Stellenbosch University, Private Bag X1, Stellenbosch, 7600, South Africa.

The general interest in microbial ecology has skyrocketed over the past decade, driven by technical advances and by the rapidly increasing appreciation of the fundamental services that these ecosystems provide. In biotechnology, ecosystems have many more functionalities than single species, and, if properly understood and harnessed, will be able to deliver better outcomes for almost all imaginable applications. However, the complexity of microbial ecosystems and of the interactions between species has limited their applicability. Read More

View Article and Full-Text PDF

A Fully Functional ROP Fluorescent Fusion Protein Reveals Roles for This GTPase in Subcellular and Tissue-Level Patterning.

Plant Cell 2020 Nov;32(11):3436-3451

Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755.

Rho of Plants (ROPs) are GTPases that regulate polarity and patterned wall deposition in plants. As these small, globular proteins have many interactors, it has been difficult to ensure that methods to visualize ROP in live cells do not affect ROP function. Here, motivated by work in fission yeast (Schizosaccharomyces pombe), we generated a fluorescent moss (Physcomitrium [Physcomitrella] patens) ROP4 fusion protein by inserting mNeonGreen after Gly-134. Read More

View Article and Full-Text PDF
November 2020

Structural and functional organization of the MYC transcriptional factors in Camellia sinensis.

Planta 2021 Apr 7;253(5):93. Epub 2021 Apr 7.

State Key Laboratory for Managing Biotic and Chemical Threats To the Quality and Safety of Agro-Products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Hangzhou, 310021, China.

Main Conclusion: Genome-wide identification, expression analysis of the MYC family in Camellia sinensis, and potential functional characterization of CsMYC2.1 have laid a solid foundation for further research on CsMYC2.1 in jasmonate (JA)-mediated response. Read More

View Article and Full-Text PDF

Shared Components of the FRQ-Less Oscillator and TOR Pathway Maintain Rhythmicity in .

J Biol Rhythms 2021 Apr 7:748730421999948. Epub 2021 Apr 7.

Department of Biology, York University, Toronto, ON, Canada.

Molecular models for the endogenous oscillators that drive circadian rhythms in eukaryotes center on rhythmic transcription/translation of a small number of "clock genes." Although substantial evidence supports the concept that negative and positive transcription/translation feedback loops (TTFLs) are responsible for regulating the expression of these clock genes, certain rhythms in the filamentous fungus continue even when clock genes (, , and ) are not rhythmically expressed. Identification of the rhythmic processes operating outside of the TTFL has been a major unresolved area in circadian biology. Read More

View Article and Full-Text PDF

Characterization of MYB35 regulated methyl jasmonate and wound responsive Geraniol 10-hydroxylase-1 gene from Bacopa monnieri.

Planta 2021 Apr 5;253(5):89. Epub 2021 Apr 5.

Biotechnology Division (CSIR-CIMAP), Central Institute of Medicinal and Aromatic Plants, (CSIR-CIMAP), P.O. CIMAP, Near Kukrail Picnic Spot, Lucknow, 226015, India.

Main Conclusion: BmG10H-1 transcript from B. monnieri was functionally active. BmG10H-1 promoter drives GUS activity in response to MeJA and wounding. Read More

View Article and Full-Text PDF

When the Ends Justify the Means: Regulation of Telomere Addition at Double-Strand Breaks in Yeast.

Front Cell Dev Biol 2021 18;9:655377. Epub 2021 Mar 18.

Department of Biological Sciences, Vanderbilt University, Nashville, TN, United States.

Telomeres, repetitive sequences located at the ends of most eukaryotic chromosomes, provide a mechanism to replenish terminal sequences lost during DNA replication, limit nucleolytic resection, and protect chromosome ends from engaging in double-strand break (DSB) repair. The ribonucleoprotein telomerase contains an RNA subunit that serves as the template for the synthesis of telomeric DNA. While telomere elongation is typically primed by a 3' overhang at existing chromosome ends, telomerase can act upon internal non-telomeric sequences. Read More

View Article and Full-Text PDF

Is a Direct Target of AGAMOUS and WUSCHEL and Is Specifically Expressed in the Floral Meristematic Cells.

Front Plant Sci 2021 18;12:642879. Epub 2021 Mar 18.

Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.

The specified floral meristem will develop a pre-established number of floral organs and, thus, terminate the floral meristematic cells. The floral meristematic pool of cells is controlled, among some others, by WUSCHEL (WUS) and AGAMOUS (AG) transcription factors (TFs). Here, we demonstrate that the () gene, a cell proliferation regulator, starts to be expressed since the floral meristem specification of and is expressed in all floral meristematic cells. Read More

View Article and Full-Text PDF

The Light-Induced WD40-Repeat Transcription Factor DcTTG1 Regulates Anthocyanin Biosynthesis in .

Front Plant Sci 2021 17;12:633333. Epub 2021 Mar 17.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.

is used as a traditional Chinese medicine and as a raw material in functional foods. stems are green or red, and red stems are richer in anthocyanins. Light is an important environmental factor that induces anthocyanin accumulation in . Read More

View Article and Full-Text PDF

A Review of Red Yeast Rice, a Traditional Fermented Food in Japan and East Asia: Its Characteristic Ingredients and Application in the Maintenance and Improvement of Health in Lipid Metabolism and the Circulatory System.

Molecules 2021 Mar 15;26(6). Epub 2021 Mar 15.

Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari 061-0293, Japan.

Red yeast rice has been used to produce alcoholic beverages and various fermented foods in China and Korea since ancient times; it has also been used to produce (Okinawan-style fermented tofu) in Japan since the 18th century. Recently, monacolin K (lovastatin) which has cholesterol-lowering effects, was found in some strains of fungi. Since statins have been used world-wide as a cholesterol-lowering agent, processed foods containing natural statins are drawing attention as materials for primary prevention of life-style related diseases. Read More

View Article and Full-Text PDF

Controlling Unconventional Secretion for Production of Heterologous Proteins in through Transcriptional Regulation and Chemical Inhibition of the Kinase Don3.

J Fungi (Basel) 2021 Mar 3;7(3). Epub 2021 Mar 3.

Institute for Microbiology, Heinrich Heine University Düsseldorf, Universitätsstraße 1, 40225 Düsseldorf, Germany.

Heterologous protein production is a highly demanded biotechnological process. Secretion of the product to the culture broth is advantageous because it drastically reduces downstream processing costs. We exploit unconventional secretion for heterologous protein expression in the fungal model microorganism . Read More

View Article and Full-Text PDF

Antioxidant, Antihypertensive and Antimicrobial Properties of Phenolic Compounds Obtained from Native Plants by Different Extraction Methods.

Int J Environ Res Public Health 2021 Mar 3;18(5). Epub 2021 Mar 3.

Nutrición y Bromatología, Instituto Universitario de Investigación en Recursos Agrarios (INURA), Escuela de Ingenierías Agrarias, Universidad de Extremadura, 06007 Badajoz, Spain.

This study aims to evaluate the efficacy of two methods (agitation and ultra-sound) for extracting phenolic compounds from 15 native plants. Plant species collected in the Dehesa of Extremadura were used. The antioxidant, antihypertensive and antimicrobial activity of the phenolic extracts was investigated. Read More

View Article and Full-Text PDF

Isolation and Functional Characterization of a Salt-Responsive Calmodulin-Like Gene from Semi-Mangrove .

Int J Mol Sci 2021 Mar 27;22(7). Epub 2021 Mar 27.

Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Salt stress is a major increasing threat to global agriculture. Pongamia (), a semi-mangrove, is a good model to study the molecular mechanism of plant adaptation to the saline environment. Calcium signaling pathways play critical roles in the model plants such as Arabidopsis in responding to salt stress, but little is known about their function in Pongamia. Read More

View Article and Full-Text PDF

Potential Probiotic Strains of and Non-: Functional and Biotechnological Characteristics.

J Fungi (Basel) 2021 Mar 2;7(3). Epub 2021 Mar 2.

Analytical Chemistry and Food Technology Department, Faculty of Chemical Sciences and Technologies, Castilla-La Mancha University, 13071 Ciudad Real, Spain.

Due to the evident demand for probiotic microorganisms, a growing number of scientific studies have involved the preliminary selection of new strains, but deeper studies for knowing specific functional and biotechnological properties are needed. In the present work, twenty yeasts ( and non-) with potential probiotic characteristics, selected in previous works, were evaluated. The following assays were realized: adhesion to Caco-2/TC7 cells, prebiotic metabolisms, assimilation of cholesterol, enzymatic and antioxidant activity, and antifungal resistance. Read More

View Article and Full-Text PDF

In Vivo Production of RNA Aptamers and Nanoparticles: Problems and Prospects.

Molecules 2021 Mar 6;26(5). Epub 2021 Mar 6.

Department of Genetics and Biotechnology, Saint Petersburg State University, 199034 Saint Petersburg, Russia.

RNA aptamers are becoming increasingly attractive due to their superior properties. This review discusses the early stages of aptamer research, the main developments in this area, and the latest technologies being developed. The review also highlights the advantages of RNA aptamers in comparison to antibodies, considering the great potential of RNA aptamers and their applications in the near future. Read More

View Article and Full-Text PDF

Harnessing a Transient Gene Expression System in to Explore Plant Agrochemical Transporters.

Plants (Basel) 2021 Mar 11;10(3). Epub 2021 Mar 11.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, China.

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. -mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease () and rice polyamine uptake transporter 1 () in was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. Read More

View Article and Full-Text PDF

Analysis of the Productivity, Immunity, and Health Performance of Nile Tilapia () Broodstock-fed Dietary Fermented Extracts Sourced from (Hilyses): A Field Trial.

Animals (Basel) 2021 Mar 14;11(3). Epub 2021 Mar 14.

Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt.

The present study aimed to evaluate the effect of dietary fermented extracts sourced from (nucleotides, β-glucans and MOS) (Hilyses) on the production and health of Nile tilapia () broodstock, as well as on seed survival and performance. The trial was performed in a hatchery along the spawning season and continued in the laboratory to monitor the performance in fry and fingerlings. The broodstock were divided into two groups, (C) fed a basal diet and (H) fed 0. Read More

View Article and Full-Text PDF

Genetic interaction between glyoxylate pathway regulator UCC1 and La-motif-encoding SRO9 regulates stress response and growth rate improvement in Saccharomyces cerevisiae.

J Biochem Mol Toxicol 2021 Apr 2:e22781. Epub 2021 Apr 2.

Genome Stability Regulation Lab, School of Biotechnology, Shri Mata Vaishno Devi University, Katra, Jammu & Kashmir, India.

Nonavailability of glucose as a carbon source results in glyoxylate pathway activation, which metabolizes nonfermentable carbon for energy generation in Saccharomyces cerevisiae. Ucc1p of S. cerevisiae inhibits activation of the glyoxylate pathway by targeting Cit2p, a key glyoxylate enzyme for ubiquitin-mediated proteasomal degradation when glucose is available as a carbon source. Read More

View Article and Full-Text PDF

Medicago truncatula Yellow Stripe-Like7 encodes a peptide transporter participating in symbiotic nitrogen fixation.

Plant Cell Environ 2021 Apr 2. Epub 2021 Apr 2.

Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid. Campus de Montegancedo. Crta. M-40 km 38. 28223 Pozuelo de Alarcón (Madrid), Spain.

Yellow Stripe-Like (YSL) proteins are a family of plant transporters that are typically involved in transition metal homeostasis. Three of the four YSL clades (I, II, and IV) transport metals complexed with the non-proteinogenic amino acid nicotianamine or its derivatives. No such capability has been shown for any member of clade III but the link between these YSLs and metal homeostasis could be masked by functional redundancy. Read More

View Article and Full-Text PDF

Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis.

Sci Rep 2021 Apr 1;11(1):7411. Epub 2021 Apr 1.

Department of Biochemistry, Medical Sciences Campus, University of Puerto Rico, San Juan, PR, 00936-5067, USA.

Functional analysis of the Mtl1 protein in Saccharomyces cerevisiae has revealed that this transmembrane sensor endows yeast cells with resistance to oxidative stress through a signaling mechanism called the cell wall integrity pathway (CWI). We observed upregulation of multiple heat shock proteins (HSPs), proteins associated with the formation of stress granules, and the phosphatase subunit of trehalose 6-phosphate synthase which suggests that mtl1Δ strains undergo intrinsic activation of a non-lethal heat stress response. Furthermore, quantitative global proteomic analysis conducted on TMT-labeled proteins combined with metabolome analysis revealed that mtl1Δ strains exhibit decreased levels of metabolites of carboxylic acid metabolism, decreased expression of anabolic enzymes and increased expression of catabolic enzymes involved in the metabolism of amino acids, with enhanced expression of mitochondrial respirasome proteins. Read More

View Article and Full-Text PDF

The role of GDP-l-galactose phosphorylase in the control of ascorbate biosynthesis.

Plant Physiol 2021 Jan 25. Epub 2021 Jan 25.

Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n, E-29071 Málaga, Spain.

The enzymes involved in l-ascorbate biosynthesis in photosynthetic organisms (the Smirnoff-Wheeler [SW] pathway) are well established. Here, we analyzed their subcellular localizations and potential physical interactions and assessed their role in the control of ascorbate synthesis. Transient expression of C terminal-tagged fusions of SW genes in Nicotiana benthamiana and Arabidopsis thaliana mutants complemented with genomic constructs showed that while GDP-d-mannose epimerase is cytosolic, all the enzymes from GDP-d-mannose pyrophosphorylase (GMP) to l-galactose dehydrogenase (l-GalDH) show a dual cytosolic/nuclear localization. Read More

View Article and Full-Text PDF
January 2021

Molecular basis for high ligand sensitivity and selectivity of strigolactone receptors in Striga.

Plant Physiol 2021 Jan 5. Epub 2021 Jan 5.

Australian Research Council Centre of Excellence for Plant Success in Nature and Agriculture, School of Natural Sciences, University of Tasmania, Hobart, Tasmania 7001, Australia.

Seeds of the root parasitic plant Striga hermonthica can sense very low concentrations of strigolactones (SLs) exuded from host roots. The S. hermonthica hyposensitive to light (ShHTL) proteins are putative SL receptors, among which ShHTL7 reportedly confers sensitivity to picomolar levels of SL when expressed in Arabidopsis thaliana. Read More

View Article and Full-Text PDF
January 2021

OsYUC11-mediated auxin biosynthesis is essential for endosperm development of rice.

Plant Physiol 2020 Dec 11. Epub 2020 Dec 11.

Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, College of Agriculture, Yangzhou University, Yangzhou, China.

Auxin is a phytohormone essential for plant development. However, our understanding of auxin-regulated endosperm development remains limited. Here, we described rice YUCCA (YUC) flavin-containing monooxygenase encoding gene OsYUC11 as a key contributor to auxin biosynthesis in rice (Oryza sativa) endosperm. Read More

View Article and Full-Text PDF
December 2020

Sulfur transfer from the endophytic fungus Serendipita indica improves maize growth and requires the sulfate transporter SiSulT.

Plant Cell 2021 Jan 21. Epub 2021 Jan 21.

School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, New Delhi-110067, India.

A deficiency of the essential macronutrient sulfur leads to stunted plant growth and yield loss; however, an association with a symbiotic fungus can greatly improve nutrient uptake by the host plant. Here, we identified and functionally characterized a high-affinity sulfate transporter from the endophytic fungus Serendipita indica. SiSulT fulfills all the criteria expected of a functional sulfate transporter responding to sulfur limitation: SiSulT expression was induced when S. Read More

View Article and Full-Text PDF
January 2021

Functional Analysis of Wheat NAC Transcription Factor, , in Regulating Resistance of Wheat to Leaf Rust Fungus.

Front Plant Sci 2021 15;12:604797. Epub 2021 Mar 15.

College of Plant Protection, Hebei Agricultural University/Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Baoding, China.

NAC transcription factors are one of the largest transcription factor families having functions in a variety of stress responses. Few NACs have been reported for interactions between wheat and the wheat rust fungus (). In this study, based on analysis of RNA-seq data from wheat line TcLr19 inoculated by , the NAC transcription factor was cloned from wheat, and its transcriptional activity and homologous dimer formation were verified. Read More

View Article and Full-Text PDF