172,409 results match your criteria yeast budding


Nse5/6 is a negative regulator of the ATPase activity of the Smc5/6 complex.

Nucleic Acids Res 2021 Apr 13. Epub 2021 Apr 13.

Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Falmer, UK.

The multi-component Smc5/6 complex plays a critical role in the resolution of recombination intermediates formed during mitosis and meiosis, and in the cellular response to replication stress. Using recombinant proteins, we have reconstituted a series of defined Saccharomyces cerevisiae Smc5/6 complexes, visualised them by negative stain electron microscopy, and tested their ability to function as an ATPase. We find that only the six protein 'holo-complex' is capable of turning over ATP and that its activity is significantly increased by the addition of double-stranded DNA to reaction mixes. Read More

View Article and Full-Text PDF

Substitution of histone H3 arginine 72 to alanine leads to deregulation of isoleucine biosynthesis in budding yeast Saccharomyces cerevisiae.

Biochem Cell Biol 2021 Apr 11. Epub 2021 Apr 11.

IISER Bhopal, 189785, Bhopal, India, 462066;

Histone residues play an essential role in the regulation of various biological processes. In the present study, we have utilized the H3/H4 histone mutant library to probe functional aspects of histone residues in amino acid biosynthesis. We found that histone residue H3R72 plays a crucial role in the regulation of isoleucine biosynthesis. Read More

View Article and Full-Text PDF

Rad9, a 53BP1 Ortholog of Budding Yeast, Is Insensitive to Spo11-Induced Double-Strand Breaks During Meiosis.

Front Cell Dev Biol 2021 25;9:635383. Epub 2021 Mar 25.

Institute for Protein Research, Osaka University, Suita, Japan.

Exogenous double-strand breaks (DSBs) induce a DNA damage response during mitosis as well as meiosis. The DNA damage response is mediated by a cascade involving Mec1/Tel1 (ATR/ATM) and Rad53 (Chk2) kinases. Meiotic cells are programmed to form DSBs for the initiation of meiotic recombination. Read More

View Article and Full-Text PDF

Co-translational assembly and localized translation of nucleoporins in nuclear pore complex biogenesis.

Mol Cell 2021 Apr 4. Epub 2021 Apr 4.

Université de Paris, CNRS, Institut Jacques Monod, 75006 Paris, France. Electronic address:

mRNA translation is coupled to multiprotein complex assembly in the cytoplasm or to protein delivery into intracellular compartments. Here, by combining systematic RNA immunoprecipitation and single-molecule RNA imaging in yeast, we have provided a complete depiction of the co-translational events involved in the biogenesis of a large multiprotein assembly, the nuclear pore complex (NPC). We report that binary interactions between NPC subunits can be established during translation, in the cytoplasm. Read More

View Article and Full-Text PDF

The rDNA is biomolecular condensate formed by polymer-polymer phase separation and is sequestered in the nucleolus by transcription and R-loops.

Nucleic Acids Res 2021 Apr 9. Epub 2021 Apr 9.

Biology Department, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

The nucleolus is the site of ribosome biosynthesis encompassing the ribosomal DNA (rDNA) locus in a phase separated state within the nucleus. In budding yeast, we find the rDNA locus and Cdc14, a protein phosphatase that co-localizes with the rDNA, behave like a condensate formed by polymer-polymer phase separation, while ribonucleoproteins behave like a condensate formed by liquid-liquid phase separation. The compaction of the rDNA and Cdc14's nucleolar distribution are dependent on the concentration of DNA cross-linkers. Read More

View Article and Full-Text PDF

Biochemical Characterization of the Num1-Mdm36 Complex at the Mitochondria-Plasma Membrane Contact Site.

Mol Cells 2021 Apr 8. Epub 2021 Apr 8.

Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 08826, Korea.

In eukaryotic cells, organelles are distributed and positioned in proximity to each other through molecular tether proteins. Among these, the mitochondria-endoplasmic reticulum cortex anchor (MECA) is a well-known tethering complex in that tethers mitochondria to the plasma membrane and plays a key role in mitochondrial fission. The main components of MECA are Num1 and Mdm36, and it is known that Mdm36 binds to Num1 to enhance mitochondrial tethering. Read More

View Article and Full-Text PDF

Cell volume homeostatically controls the rDNA repeat copy number and rRNA synthesis rate in yeast.

PLoS Genet 2021 Apr 7;17(4):e1009520. Epub 2021 Apr 7.

Instituto de Biotecnología y Biomedicina (Biotecmed), Universitat de València, Burjassot, Spain.

The adjustment of transcription and translation rates to the changing needs of cells is of utmost importance for their fitness and survival. We have previously shown that the global transcription rate for RNA polymerase II in budding yeast Saccharomyces cerevisiae is regulated in relation to cell volume. Total mRNA concentration is constant with cell volume since global RNApol II-dependent nascent transcription rate (nTR) also keeps constant but mRNA stability increases with cell size. Read More

View Article and Full-Text PDF

Atg27p co-fractionates with clathrin-coated vesicles in budding yeast.

MicroPubl Biol 2021 Mar 29;2021. Epub 2021 Mar 29.

Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL, USA 33101.

Atg27p, a single-pass transmembrane protein that functions in autophagy, localizes to a variety of cellular compartments including the pre-autophagosomal structure, late Golgi, vacuolar membrane, as well as early and late endosomes. Its cytoplasmic C-terminus contains a tyrosine sorting motif that allows for its transport to the vacuolar membrane and an additional sequence that allows for its retrieval from the vacuolar membrane to the endosome. Since clathrin is well known to mediate vesicular transport in the endomembrane system, the trafficking of Atg27p and its tyrosine sorting motif suggested that it might be trafficked inside clathrin-coated vesicles (CCVs). Read More

View Article and Full-Text PDF

The Vps13 Family of Lipid Transporters and Its Role at Membrane Contact Sites.

Int J Mol Sci 2021 Mar 12;22(6). Epub 2021 Mar 12.

Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, BC V5Z 4H4, Canada.

The conserved VPS13 proteins constitute a new family of lipid transporters at membrane contact sites. These large proteins are suspected to bridge membranes and form a direct channel for lipid transport between organelles. Mutations in the 4 human homologs () are associated with a number of neurological disorders, but little is known about their precise functions or the relevant contact sites affected in disease. Read More

View Article and Full-Text PDF

Biological and Physico-Chemical Characteristics of Arginine-Rich Peptide Gemini Surfactants with Lysine and Cystine Spacers.

Int J Mol Sci 2021 Mar 24;22(7). Epub 2021 Mar 24.

Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, 80-416 Gdańsk, Poland.

Ultrashort cationic lipopeptides (USCLs) and gemini cationic surfactants are classes of potent antimicrobials. Our recent study has shown that the branching and shortening of the fatty acids chains with the simultaneous addition of a hydrophobic -terminal amino acid in USCLs result in compounds with enhanced selectivity. Here, this approach was introduced into arginine-rich gemini cationic surfactants. Read More

View Article and Full-Text PDF

Comparing Transcriptome Profiles of Saccharomyces Cerevisiae Cells Exposed to Cadmium Selenide/Zinc Sulfide and Indium Phosphide/Zinc Sulfide.

Genes (Basel) 2021 Mar 17;12(3). Epub 2021 Mar 17.

Department of Biology, Missouri State University, 901 S National, Springfield, MO 65897, USA.

The primary focus of our research was to obtain global gene expression data in baker's yeast exposed to sub-lethal doses of quantum dots (QDs), such as green-emitting CdSe/ZnS and InP/ZnS, to reveal novel insights on their unique mechanisms of toxicity. Despite their promising applications, their toxicity and long-lasting effects on the environment are not well understood. To assess toxicity, we conducted cell viability assays, ROS detection assays, and assessed their effects on the trafficking of Vps10-GFP toward the -Golgi network with confocal microscopy. Read More

View Article and Full-Text PDF

Developing a Yeast Platform Strain for an Enhanced Taxadiene Biosynthesis by CRISPR/Cas9.

Metabolites 2021 Mar 3;11(3). Epub 2021 Mar 3.

Department of Biological Science, University of Calgary, Calgary, AB T2N1N4, Canada.

Paclitaxel is an important diterpenoid commonly used as an anticancer drug. Although the paclitaxel biosynthetic pathway has been mostly revealed, some steps remain to be elucidated. The difficulties in plant transformations and the scarcity of the precursor of paclitaxel, (+)-taxa-4(5), 11(12)-diene (taxadiene), have hindered the full comprehension of paclitaxel biochemistry and, therefore, its production by biotechnological approaches. Read More

View Article and Full-Text PDF

Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network.

Nat Commun 2021 04 1;12(1):2034. Epub 2021 Apr 1.

Institute of Structural and Molecular Biology, Birkbeck College, London, UK.

COPII mediates Endoplasmic Reticulum to Golgi trafficking of thousands of cargoes. Five essential proteins assemble into a two-layer architecture, with the inner layer thought to regulate coat assembly and cargo recruitment, and the outer coat forming cages assumed to scaffold membrane curvature. Here we visualise the complete, membrane-assembled COPII coat by cryo-electron tomography and subtomogram averaging, revealing the full network of interactions within and between coat layers. Read More

View Article and Full-Text PDF

Ccr4-Not as a mediator of environmental signaling: a jack of all trades and master of all.

Curr Genet 2021 Mar 31. Epub 2021 Mar 31.

Department of Pathology and Laboratory Medicine, College of Medicine and the Center for Cancer Research, University of Tennessee Health Science Center, Room 318, 19 South Manassas, Memphis, TN, 38163, USA.

The cellular response to environmental exposures, such as nutrient shifts and various forms of stress, requires the integration of the signaling apparatus that senses these environmental changes with the downstream gene regulatory machinery. Delineating this molecular circuitry remains essential for understanding how organisms adapt to environmental flux, and it is critical for determining how dysregulation of these mechanisms causes disease. Ccr4-Not is a highly conserved regulatory complex that controls all aspects of the gene expression process. Read More

View Article and Full-Text PDF

Inactivating histone deacetylase HDA promotes longevity by mobilizing trehalose metabolism.

Nat Commun 2021 03 31;12(1):1981. Epub 2021 Mar 31.

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.

Histone acetylations are important epigenetic markers for transcriptional activation in response to metabolic changes and various stresses. Using the high-throughput SEquencing-Based Yeast replicative Lifespan screen method and the yeast knockout collection, we demonstrate that the HDA complex, a class-II histone deacetylase (HDAC), regulates aging through its target of acetylated H3K18 at storage carbohydrate genes. We find that, in addition to longer lifespan, disruption of HDA results in resistance to DNA damage and osmotic stresses. Read More

View Article and Full-Text PDF

Acute ethanol stress induces sumoylation of conserved chromatin structural proteins in .

Mol Biol Cell 2021 Mar 31:mbcE20110715. Epub 2021 Mar 31.

Department of Pharmacology, University of Washington, Seattle, WA, 98195, USA.

Stress is ubiquitous to life and can irreparably damage essential biomolecules and organelles in cells. To survive, organisms must sense and adapt to stressful conditions. One highly conserved adaptive stress response is through the post-translational modification of proteins by the small ubiquitin-like modifier (SUMO). Read More

View Article and Full-Text PDF

Epidemiology and risk factors of candidemia due to Candida parapsilosis in an intensive care unit.

Rev Inst Med Trop Sao Paulo 2021 24;63:e20. Epub 2021 Mar 24.

Wuhan University, Renmin Hospital, Department of Respiratory and Critical Care Medicine, Wuhan, Hubei Province, People's Republic of China.

We analyzed the clinical features and risk factors of candidemia due to C. parapsilosis (n=104) in the intensive care unit of a tertiary hospital over six years. This was a monocentric, retrospective study of candidemia, conducted from January 2013 to March 2019. Read More

View Article and Full-Text PDF

A Case of Histoplasmosis Mimicking Primary Lung Cancer With Liver Metastasis.

Cureus 2021 Feb 23;13(2):e13517. Epub 2021 Feb 23.

Internal Medicine, Sanford Health, University of North Dakota School of Medicine, Fargo, USA.

Histoplasmosis is usually self-limiting in healthy individuals but often fatal in immunocompromised patients. It can mimic primary lung malignancy and liver metastasis, causing a delay in appropriate therapy. We report a case of a 58-year-old male, with a 20 pack-year smoking habit, who presented with a three-week history of persistent fevers and productive cough with night sweats. Read More

View Article and Full-Text PDF
February 2021

Carotenoid Production in Oleaginous Yeasts.

Adv Exp Med Biol 2021 ;1261:153-163

Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Ishikawa, Japan.

Oleaginous yeasts, Yarrowia lipolytica and Lipomyces starkeyi, can synthesize more than 20% of lipids per dry cell weight from a wide variety of substrates. This feature is attractive for cost-efficient production of industrial biodiesel fuel. These yeasts are also very promising hosts for the efficient production of more value-added lipophilic compound carotenoids, e. Read More

View Article and Full-Text PDF

Selenium supplementation inhibits IGF-1 signaling and confers methionine restriction-like healthspan benefits to mice.

Elife 2021 Mar 30;10. Epub 2021 Mar 30.

Department of Biology, Orentreich Foundation for the Advancement of Science, Cold Spring, United States.

Methionine restriction (MR) dramatically extends the healthspan of several organisms. Methionine-restricted rodents have less age-related pathology and increased longevity as compared with controls, and recent studies suggest that humans might benefit similarly. Mechanistically, it is likely that the decreased IGF-1 signaling that results from MR underlies the benefits of this regimen. Read More

View Article and Full-Text PDF

[Progress in studies on production of chemicals from xylose by Saccharomyces cerevisiae].

Sheng Wu Gong Cheng Xue Bao 2021 Mar;37(3):1042-1057

State Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, Shandong, China.

Effective utilization of xylose is a basis for economic production of biofuels or chemicals from lignocellulose biomass. Over the past 30 years, through metabolic engineering, evolutionary engineering and other strategies, the metabolic capacity of xylose of the traditional ethanol-producing microorganism Saccharomyces cerevisiae has been significantly improved. In recent years, the reported results showed that the transcriptome and metabolome profiles between xylose and glucose metabolism existed significant difference in recombinant yeast strains. Read More

View Article and Full-Text PDF

[Advances in metabolic engineering of methylotrophic yeasts].

Sheng Wu Gong Cheng Xue Bao 2021 Mar;37(3):966-979

Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.

Methylotrophic yeasts are considered as promising cell factories for bio-manufacturing due to their several advantages such as tolerance to low pH and high temperature. In particular, their methanol utilization ability may help to establish a methanol biotransformation process, which will expand the substrate resource for bio-refinery and the product portfolio from methanol. This review summarize current progress on engineering methylotrophic yeasts for production of proteins and chemicals, and compare the strengths and weaknesses with the model yeast Saccharomyces cerevisiae. Read More

View Article and Full-Text PDF