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Foods 2022 May 3;11(9). Epub 2022 May 3.

School of Food Engineering, University of Campinas, Rua Monteiro Lobato, 80, Campinas 13083-862, SP, Brazil.

Whey beverages that were enriched with fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were used for carrying extract that was obtained by the supercritical CO extraction technique to produce novel functional products. Fennel-based whey beverages were subjected to thermosonication processing (100, 200, and 300 W at 60 °C for 15 min) to verify the performance of the dairy colloidal system for protecting the bioactive fennel compounds. The impacts of thermosonication processing on the quality attributes of the functional whey beverages were examined according to their droplet size distribution, microstructure, kinetic stability, color parameters, browning index, total phenolic content (TPC), and antioxidant capacity by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2-Azino-bis-(3-ethylbenzothiazoline)-6-sulphonic acid) assays. Read More

Bioresour Technol 2022 Jul 5;355:127269. Epub 2022 May 5.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China; College of Forestry, Northwest A&F University, Yangling 712100, China. Electronic address:

Acid salts have been shown to catalyze xylan hydrolysis selectively and efficiently for xylooligosaccharides (XOS) production while using acid salts that are non-toxic and available as feed additives can avoid separation from resulting XOS-rich hydrolysates. There is no report on XOS production with sodium bisulfate (NaHSO) hydrolysis, of significance is that NaHSO as feed additive does not need to be separated. In this work, NaHSO hydrolysis was firstly employed to produce XOS from poplar. Read More

Appl Biochem Biotechnol 2022 May 6. Epub 2022 May 6.

Department of Applied Chemistry, Delhi Technological University, Delhi, 110042, India.

Herein, we envisage the fabrication of a highly sensitive enzymatic electrochemical biosensor for selective detection of xanthine (Xn) using xanthine oxidase (XOs) immobilized polyaniline-wrapped titanium dioxide ([email protected]) nanohybrid as a sensing platform. The [email protected] nanohybrid was synthesized via chemical polymerization using ammonium per sulfate as an oxidant. Various microscopic, spectroscopic, and electrochemical techniques have been utilized to confirm the electrophoretic deposition of the PANI and [email protected] nanohybrids on to indium tin oxide (ITO) coated glass substrate. Read More

Braz J Microbiol 2022 Apr 28. Epub 2022 Apr 28.

Center of Medical Sciences and Pharmaceutical, Western Paraná State University, Rua Universitária, 2069, Cascavel, PR, ZC 85814-110, Brazil.

Purified endoxylanase from Thermomyces lanuginosus PC7S1T was immobilized in calcium alginate, resulting in a yield of 78.5% and a reusability for 11 cycles. The stability of the immobilized enzyme was given for a pH range of 4 to 9 for 96 h. Read More

Bioresour Technol 2022 Jun 25;354:127225. Epub 2022 Apr 25.

Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.

Bamboo processing residue, which is rich in parenchyma cells, was treated as huge waste in bamboo processing industry, such as reassemble bamboo and bamboo flooring. Herein, autohydrolysis and rapid different deep eutectic solvents (DES) delignification strategy were consecutively performed to remove hemicelluloses and lignin from bamboo processing residue. The xylooligosaccharides (XOS) with high yield (34. Read More

Indian J Microbiol 2022 Jun 29;62(2):317-321. Epub 2022 Mar 29.

Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana 124001 India.

Xylooligosaccharides are known prebiotics that various foods and feed industries can utilize. In the present study, the xylan was extracted from sugarcane bagasse and coconut coir by NaOH treatment and it was further structurally characterized by FTIR. Furthermore, VAPS-24 was used to produce endo-xylanase, which induced the production of XOS from both of these substrates. Read More

Molecules 2022 Apr 13;27(8). Epub 2022 Apr 13.

Department of Biological Science and Biotechnology, University of Jinan, Jinan 250022, China.

A xylanase-producing strain, identified as sp. T7, was isolated from soil by our lab. The endo-β-1,4-xylanase (ST7) gene was found in the genome sequence of strain T7, which was cloned and expressed in . Read More

Biology (Basel) 2022 Apr 2;11(4). Epub 2022 Apr 2.

Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăstur 3-5, 400372 Cluj-Napoca, Romania.

Xylo-oligosaccharides (XOS) are considered non-digestible fibers produced mainly from agricultural biomass and are classified as "emerging prebiotic" compounds. Since XOS were shown to promote the growth of bifidobacteria in the gut with potential effects on one's health, scientists used them as food ingredients. For example, the addition of XOS in bakery products could improve their physicochemical characteristics. Read More

Bioresour Technol 2022 Jun 19;354:127190. Epub 2022 Apr 19.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China; College of Forestry, Northwest A&F University, Yangling 712100, China. Electronic address:

Organic acid hydrolysis is a potential method for xylooligosaccharides (XOS) production from lignocelluloses. However, the effect of lignin content on XOS production using organic acid hydrolysis remains unclear. In this work, the effect of delignification on XOS production from poplar by acetic acid (AC) hydrolysis was investigated. Read More

RSC Adv 2021 Mar 23;11(20):11882-11888. Epub 2021 Mar 23.

Lund University, Division of Biotechnology, Department of Chemistry Lund Sweden

The enzymatic production of prebiotic xylooligosaccharides (XOS) has become an attractive way to valorise lignocellulosic biomass. However, despite numerous xylanases reported for potential use in the production of XOS, most of the family GH10 also produce xylose. This monosaccharide can negatively affect the selectivity to stimulate the growth of intestinal microorganisms beneficial to human health. Read More

Biotechnol Biofuels Bioprod 2022 Jan 7;15(1). Epub 2022 Jan 7.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.

Background: Autohydrolysis is an extensively investigated pretreatment method due to its environmental friendliness. During autohydrolysis, most xylan from hemicellulose can be converted into xylooligosaccharides (XOS), and cellulose in the autohydrolyzed residues can be transformed into glucose after enzymatic hydrolysis. Both of these are value-added biochemicals in the biorefining process. Read More

Food Chem (Oxf) 2021 Dec 13;3:100040. Epub 2021 Sep 13.

Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), College of Life Sciences, East China Normal University, Shanghai 200241, China.

High level of carbohydrate in aquafeed could achieve cost-sparing effect, but it may cause adverse effects on flesh quality of aquatic products. An eight-week trial was conducted to investigate whether oligosaccharides-supplementation, including Galacto-oligosaccharides (GOS) and xylo-oligosaccharide (XOS), could systematically improve the growth performance, texture characteristics and nutrition composition of Nile tilapia fed with high-carbohydrate diet. The results indicated that GOS-supplementation improved the amino acid composition, while XOS-supplementation showed beneficial effects on growth performance. Read More

Int J Biol Macromol 2022 Jun 6;209(Pt A):188-197. Epub 2022 Apr 6.

Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China. Electronic address:

To achieve an effective deconstruction for preparation of xylooligosaccharides (XOS) and lignin nanoparticles (LNPs) from Eucommia ulmoides, a synergistic pretreatment was successfully developed. Herein, the hemicelluloses were preferentially dissociated in acetic acid-catalyzed hydrothermal pretreatment (HTP) for preparation of XOS, and the hydrothermally-pretreated substrate was then subjected to deep eutectic solvents (DES) delignification for fabrication of LNPs. Results showed that the optimal yield (33. Read More

Bioresour Technol 2022 Apr 1:127093. Epub 2022 Apr 1.

Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Plas Gogerddan, Aberystwyth SY23 3EE, UK.

This study investigated the production of xylo-oligosaccharides (XOS) from sugarcane straw (SCS) using steam explosion (SE) pretreatment at pilot-scale, as well as co-production of other fermentable sugars and lignin-rich residues for bioethanol and bioenergy, respectively. SE conditions 200 °C; 15 bar; 10 min led to 1) soluble XOS yields of up to 35% (w/w) of initial xylan with ∼50% of the recovered XOS corresponding to xylobiose and xylotriose, considered the most valuable sugars for prebiotic applications; 2) fermentable glucose yields from the enzymatic hydrolysis of SE-pretreated SCS of up to ∼78%; 3) increase in the energy content of saccharified SCS residues (16%) compared to the untreated material. From an integrated biorefinery perspective, it demonstrated the potential use of SCS for the production of value-added XOS ingredients as well as liquid and solid biofuel products. Read More

Int J Mol Sci 2022 Mar 10;23(6). Epub 2022 Mar 10.

Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR47UH, UK.

Xylan is one of the major structural components of the plant cell wall. Xylan present in the human diet reaches the large intestine undigested and becomes a substrate to species of the gut microbiota. Here, we characterised the capacity of and strains to utilise xylan derivatives. Read More

Bioresour Technol 2022 May 19;352:127041. Epub 2022 Mar 19.

Bioresource Processing Research Institute of Australia (BioPRIA), Department of Chemical and Biological Engineering, Monash University, Clayton, VIC 3800, Australia; ARC Industrial Transformation Research Hub for Processing Advanced Lignocellulosics (PALS), Department of Chemical and Biological Engineering, Monash University, Clayton, VIC 3800, Australia; Department of Chemical and Biological Engineering, Monash University, Clayton, VIC 3800, Australia. Electronic address:

Generation of specific xylooligosaccharides (XOS) is attractive to the pharmaceutical and food industries due to the importance of their structure upon their application. This study used chemometrics to develop a comprehensive computational modelling set to predict the parameters maximising the generation of the desired XOS during enzymatic hydrolysis. The evaluated parameters included pH, temperature, substrate concentration, enzyme dosage and reaction time. Read More

Appl Biochem Biotechnol 2022 Mar 17. Epub 2022 Mar 17.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, No. 159 Longpan Road, 210037, Nanjing, People's Republic of China.

Xylo-oligosaccharides have great value in food, feed fields. Previous studies have shown that organic acids catalyze the hydrolysis of xylan-rich sources for the production of xylo-oligosaccharides. In this study, gluconic acid of aldonic acid generated xylo-oligosaccharides via hydrolysis of xylan from corncob. Read More

Carbohydr Polym 2022 Jun 5;285:119217. Epub 2022 Feb 5.

College of Forestry, Northwest A&F University, Yangling 712100, China; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China. Electronic address:

Xylan extraction by alkali is usually a prerequisite for traditional xylooligosaccharide (XOS) production from corncob. In this study, to avoid xylan isolation before XOS production, corncob was delignified by hydrogen peroxide-acetic acid (HPAA), followed by a two-step enzymatic hydrolysis for XOS and monosaccharide production. High lignin removal (55. Read More

J Microbiol Biotechnol 2022 Feb 24;32(5):1-8. Epub 2022 Feb 24.

Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea.

The saccharification of cellulose and hemicellulose is essential for utilizing lignocellulosic biomass as a biofuel. While cellulose is composed of glucose only, hemicelluloses are composed of diverse sugars such as xylose, arabinose, glucose, and galactose. is a good potential candidate for hemicellulose biofuel production as this archaeon simultaneously utilizes various sugars. Read More

J Sci Food Agric 2022 Feb 26. Epub 2022 Feb 26.

Department of Food Engineering, University of Gaziantep, Gaziantep, Turkey.

Background: Bulgur bran (BB) is a potential source for the production of value-added products such as fermentable sugars and xylooligosaccharides (XOs). In this study, alkali combined twin-screw extrusion pretreatment was performed and statistically optimized to enhance fractionation and enzymatic hydrolysis of BB. The pretreatment conditions (barrel temperature, screw speed and alkali impregnation) were optimized by Box-Behnken design (BBD) to obtain the highest hemicellulose separation from BB. Read More

Polymers (Basel) 2022 Feb 21;14(4). Epub 2022 Feb 21.

College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.

There is an increasing emphasis on the transformation of lignocellulosic biomass into versatile products. The feasibility of preparing xylooligosaccharides (XOS) by hydrolysis of sorghum stalk (SS) using organic and inorganic acids was studied. The influences of different acids (gluconic acid, acetic acid, sulfuric acid, and oxalic acid), process time and temperature on the hydrolysis of SS were explored. Read More

Eur J Nutr 2022 Feb 23. Epub 2022 Feb 23.

Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.

Purpose: Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats. However, the gut microbiota composition in these rats has not been investigated. Therefore, this study aimed to investigate the impact of biotic therapies on gut microbiota in HFD-induced obese-insulin-resistant rats. Read More

Comp Biochem Physiol C Toxicol Pharmacol 2022 Jun 19;256:109296. Epub 2022 Feb 19.

State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha 410081, Hunan, China. Electronic address:

In this study, a total of 420 healthy crucian carp (9.77 ± 0.04 g) were randomly divided into CK, B·S, XOS and B·S + XOS group, and cultured for 8 weeks. Read More

Bioresour Technol 2022 Apr 17;349:126866. Epub 2022 Feb 17.

Department of Chemistry and Chemical Engineering, MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, National Forest and Grass Administration Woody Spices (East China) Engineering Technology Research Center, Beijing Forestry University, Beijing 100083, PR China. Electronic address:

High pretreatment temperature is necessary to obtain xylo-oligosaccharides (XOS) with low degree of polymerization (DP). However, traditional isothermal pretreatment for XOS production may increase the generation of xylose and furfural with the reaction time extending (10-100 min). In this study, non-isothermal subcritical CO-assisted seawater autohydrolysis (NSCSA) firstly used seawater and CO for the coproduction of XOS with low DP and glucose. Read More

Int J Mol Sci 2022 Jan 26;23(3). Epub 2022 Jan 26.

Department of Microbial & Plan Biotechnology, Centro de Investigaciones Biológicas Margarita Salas, Spanish National Research Council (CSIC), C/Ramiro de Maeztu 9, 28040 Madrid, Spain.

The study of endoxylanases as catalysts to valorize hemicellulosic residues and to obtain glycosides with improved properties is a topic of great industrial interest. In this work, a GH10 β-1,4-endoxylanase (XynSOS), from the ascomycetous fungus , has been heterologously produced in , purified, and characterized. rXynSOS is a highly glycosylated monomeric enzyme of 53 kDa that contains a functional CBM1 domain and shows its optimal activity on azurine cross-linked (AZCL)-beechwood xylan at 70 °C and pH 5. Read More

J Biotechnol 2022 Mar 10;347:1-8. Epub 2022 Feb 10.

Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José do Rio Preto, São Paulo, Brazil. Electronic address:

Xylooligosaccharides (XOs) are a promising class of prebiotics capable of selectively stimulating the growth of the beneficial intestinal microbiota against intestinal pathogens. They can be obtained from xylan present in residual lignocellulosic material from agriculture. Thus, in this study we produced XOs by extracting xylan from sugarcane bagasse and hydrolyzing it using the GH10 xylanase from Thermoascus aurantiacus expressed by Pichia pastoris. Read More

World J Microbiol Biotechnol 2022 Feb 8;38(3):52. Epub 2022 Feb 8.

School of Chemistry and Food, Federal University of Rio Grande, Avenue Italia Km 8, Rio Grande, Brazil.

Xylanolytic enzymes are involved in xylan hydrolysis, the main ones being endo-β-1,4-xylanases (xylanases). This can be applied in the bioconversion of lignocellulosic materials into value-added products such as xylooligosaccharides (XOS). This study aimed to establish a protocol for the purification of xylanases, as well as to characterize and apply the purified enzyme extract in the production of XOS. Read More

J Anim Sci Biotechnol 2022 Feb 8;13(1):13. Epub 2022 Feb 8.

Department of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, 1955 East-West Rd, Honolulu, HI, 96822, USA.

Background: This study investigated a novel in ovo feeding strategy to determine the prebiotic effects of xylo- and mannan- oligosaccharides (XOS and MOS) differing in the degree of polymerization. A total of 192 fertilized eggs were divided into 6 treatment groups: i) normal saline control (NSC), ii) xylotriose (XOS3), iii) xylotetraose (XOS4), iv) mannotriose (MOS3), v) mannotetraose (MOS4), and vi) no injection control (NIC), each containing 4 replicate trays with 8 eggs per replicate. On d 17 of incubation, 3 mg of oligosaccharides (except for controls) dissolved in 0. Read More

Biotechnol Rep (Amst) 2022 Mar 21;33:e00702. Epub 2022 Jan 21.

School of Life Sciences, College of Agriculture Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban 4000, South Africa.

Functional foods are essential food products that possess health-promoting properties for the treatment of infectious diseases. In addition, they provide energy and nutrients, which are required for growth and survival. They occur as prebiotics or dietary supplements, including oligosaccharides, processed foods, and herbal products. Read More

BMC Biotechnol 2022 02 5;22(1). Epub 2022 Feb 5.

Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Science, Jiangsu Normal University, No. 101, Shanghai Road, Tongshan District, Xuzhou, 221116, Jiangsu Province, China.

Background: One-step fermentation is a cheap way to produce xylo-oligosaccharides (XOS), where production of xylanases and XOS is integrated into a single process. In spite of cost advantage, one-step fermentation is still short in yield so far due to the limited exploration. To cope with this issue, production of XOS from wheat bran by recombinant Escherichia coli through one-step fermentation was investigated in this study. Read More