4,665 results match your criteria xenografted mice

Pathways and factors regulated by bone marrow-derived stem cells in human ovarian tissue.

Fertil Steril 2021 May 8. Epub 2021 May 8.

Fundación Instituto Valenciano de Infertilidad (IVI), Valencia, Spain; Grupo de Investigación en Medicina Reproductiva, Instituto de Investigación Sanitaria La Fe (IIS La Fe), Valencia, Spain; IVI-RMA Valencia, Valencia, Spain. Electronic address:

Objective: To describe molecular and paracrine signaling changes produced by human bone marrow-derived stem cells (BMDSC) in human ovarian cortex.

Design: Experimental study.

Setting: University hospital research laboratories. Read More

View Article and Full-Text PDF

Genetic Manipulation of Sirtuin 3 Causes Alterations of Key Metabolic Regulators in Melanoma.

Front Oncol 2021 16;11:676077. Epub 2021 Apr 16.

Department of Dermatology, University of Wisconsin, Madison, WI, United States.

The mitochondrial sirtuin SIRT3 plays key roles in cellular metabolism and energy production, which makes it an obvious target for the management of cancer, including melanoma. Previously, we have demonstrated that SIRT3 was constitutively upregulated in human melanoma and its inhibition resulted in anti-proliferative effects in human melanoma cells and in human melanoma xenografts. In this study, we expanded our data employing knockdown and overexpression strategies in cell culture and mouse xenografts to further validate and establish the pro-proliferative function of SIRT3 in melanocytic cells, and its associated potential mechanisms, especially focusing on the metabolic regulation. Read More

View Article and Full-Text PDF

An oncolytic adenovirus delivering TSLC1 inhibits Wnt signaling pathway and tumor growth in SMMC-7721 xenograft mice model.

Acta Biochim Biophys Sin (Shanghai) 2021 Apr 30. Epub 2021 Apr 30.

Department of Pathology, Zhejiang Provincial People's Hospital, Hangzhou 311402, China.

Tumor suppressor in lung cancer-1 (TSLC1) was first identified as a tumor suppressor for lung cancer, and frequently downregulated in various types of cancers including hepatocellular carcinoma (HCC). The Wnt pathway plays a critical role in tumorigenesis, migration, and invasion in HCC. However, the function of TSLC1 in modulating Wnt signaling in HCC is unclear. Read More

View Article and Full-Text PDF

Nucleoside-Lipid-Based Nanoparticles for Phenazine Delivery: A New Therapeutic Strategy to Disrupt Hsp27-eIF4E Interaction in Castration Resistant Prostate Cancer.

Pharmaceutics 2021 Apr 27;13(5). Epub 2021 Apr 27.

Centre de Recherche en Cancérologie de Marseille, CRCM, Inserm UMR 1068, CNRS, UMR 7258, Aix-Marseille University U105, Institut Paoli-Calmettes, F-13009 Marseille, France.

Heat shock protein 27 (Hsp27) has an established role in tumor progression and chemo-resistance of castration-resistant prostate cancer (CRPC). Hsp27 protects eukaryotic translation initiation factor 4E (eIF4E) from degradation, thereby maintaining survival during treatment. Phenazine derivative compound #14 was demonstrated to specifically disrupt Hsp27/eIF4E interaction and significantly delay castration-resistant tumor progression in prostate cancer xenografts. Read More

View Article and Full-Text PDF

Tumor Solid Stress: Assessment with MR Elastography under Compression of Patient-Derived Hepatocellular Carcinomas and Cholangiocarcinomas Xenografted in Mice.

Cancers (Basel) 2021 Apr 15;13(8). Epub 2021 Apr 15.

Laboratory of Imaging Biomarkers, Center of Research on Inflammation, Université de Paris, UMR 1149, Inserm, F-75018 Paris, France.

Malignant tumors have abnormal biomechanical characteristics, including high viscoelasticity, solid stress, and interstitial fluid pressure. Magnetic resonance (MR) elastography is increasingly used to non-invasively assess tissue viscoelasticity. However, solid stress and interstitial fluid pressure measurements are performed with invasive methods. Read More

View Article and Full-Text PDF

A438079 affects colorectal cancer cell proliferation, migration, apoptosis, and pyroptosis by inhibiting the P2X7 receptor.

Biochem Biophys Res Commun 2021 Jun 26;558:147-153. Epub 2021 Apr 26.

Department of Medical Genetics, China Medical University, Shenyang, China. Electronic address:

Background: Our previous study identified elevated expression of the P2X7 receptor (P2X7R) in colorectal cancer (CRC) patients, suggesting the receptor is a target for predicting poor disease prognosis. A438079 is a highly selective P2X7R antagonist, however, no studies have identified A438079 effects and mechanisms toward the biological behavior of CRC cells, and its therapeutic in vivo potential in CRC nude mice.

Methods: The CRC cell lines, HCT-116 and SW620 were treated with 10 μM A438079, after which proliferation, migration, invasion, and apoptosis were assessed. Read More

View Article and Full-Text PDF

EHF enhances malignancy by modulating AKT and MAPK/ERK signaling in non‑small cell lung cancer cells.

Oncol Rep 2021 Jun 28;45(6). Epub 2021 Apr 28.

Shanxi Provincial Research Center for The Project of Prevention and Treatment of Respiratory Diseases, Xi'an, Shaanxi 710061, P.R. China.

Overexpression of ETS‑homologous factor (EHF) in non‑small cell lung cancer (NSCLC) is associated with poor patient prognosis. To explore the mechanism of the effect of EHF in NSCLC, EHF expression was examined in NSCLC and its role in cell proliferation, invasion, cell cycle, and apoptosis of NSCLC cells was evaluated by overexpressing EHF and/or knocking down EHF expression in NSCLC cells and in cancer cell grafted mice . The results revealed that the knockdown of EHF expression in NSCLC with siRNA significantly inhibited cell proliferation and invasion, arrested the cell cycle at the G/G phase, and induced apoptosis, whereas overexpression of EHF in NSCLC promoted cell proliferation, tumor growth, and cancer cell migration . Read More

View Article and Full-Text PDF

Distinct Repopulation Activity in Hu-Mice Between CB- and LPB- CD34 Cells by Enrichment of Transcription Factors.

Int J Stem Cells 2021 Apr 30. Epub 2021 Apr 30.

Department of Biomedical Science, CHA University, Seongnam, Korea.

Background And Objectives: Human CD34 hematopoietic stem cells can reconstitute the human hematopoietic system when transplanted into immunocompromised mice after irradiation. Human leukapheresis peripheral blood (LPB)- and cord blood (CB)-derived CD34 cells have a similar capacity to reconstitute myeloid lineage cells in a humanized mice (hu-mice) model. However, potent stem cells, such as CB-CD34 cells, efficiently reconstitute the lymphoid system in vivo compared to LPB-CD34 cells. Read More

View Article and Full-Text PDF

hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4.

Mol Ther Nucleic Acids 2021 Jun 24;24:591-609. Epub 2021 Feb 24.

Department of Ultrasound, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun 130033, Jilin, China.

We identified a novel interactome, circ_0001018/miR-338-3p/SOX4, in papillary thyroid cancer (PTC), and we intended to confirm the regulatory relationship between the three and to study the effects of the three in PTC. The bioinformatics method was used to screen out the circular RNA and mRNA of interest. A cellular fractionation assay and fluorescence hybridization (FISH) assay were conducted to prove that circ_0001018 and CCT4 (the host gene of circ_0001018) mRNA primarily localized in the cytoplasm of PTC cell lines. Read More

View Article and Full-Text PDF

GNA14 stimulation of KLF7 promotes malignant growth of endometrial cancer through upregulation of HAS2.

BMC Cancer 2021 Apr 23;21(1):456. Epub 2021 Apr 23.

Department of obstetrics and gynecology, the First Hospital of Lanzhou University, Key Laboratory for Gynecologic Oncology Gansu Province, NO.1 Donggang West Road, Chengguan District, Lanzhou, Gansu, 730000, P. R. China.

Background: Endometrial cancer (UCEC) is one of the most common gynecological malignancies. We previously found that overexpression of G protein α subunit 14 (GNA14) promoted UCEC growth. Krüppel-like factor 7 (KLF7) acts as an oncogene in various cancer types, whereas the connection between GNA14 and KLF7 in UCEC is unclear. Read More

View Article and Full-Text PDF

Epidermal growth factor receptor regulates fibrinolytic pathway elements in cervical cancer: functional and prognostic implications.

Braz J Med Biol Res 2021 19;54(6):e10754. Epub 2021 Apr 19.

Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.

Epidermal growth factor receptor (EGFR) signaling and components of the fibrinolytic system, including urokinase-type plasminogen activator (uPA) and thrombomodulin (TM), have been implicated in tumor progression. In the present study, we employed cBioPortal platform (http://www.cbioportal. Read More

View Article and Full-Text PDF

Anti-tumoral activity of the Pan-HER (Sym013) antibody mixture in gemcitabine-resistant pancreatic cancer models.

MAbs 2021 Jan-Dec;13(1):1914883

Institut De Recherche En Cancérologie De Montpellier (IRCM), INSERM U1194, Université De Montpellier, Institut Régional Du Cancer De Montpellier (ICM), Montpellier, France.

Chemoresistance, particularly to gemcitabine, is a major challenge in pancreatic cancer. The epidermal growth factor receptor (EGFR) and human epidermal growth factor receptors 2 and 3 (HER2, HER3) are expressed in many tumors, and they are relevant therapeutic targets due to their synergistic interaction to promote tumor aggressiveness and therapeutic resistance. Cocktails of antibodies directed against different targets are a promising strategy to overcome these processes. Read More

View Article and Full-Text PDF

Radiolabeling Optimization and Preclinical Evaluation of the New PSMA Imaging Agent [F]AlF-P16-093.

Bioconjug Chem 2021 Apr 19. Epub 2021 Apr 19.

Five Eleven Pharma Inc., Philadelphia, Pennsylvania 19104, United States.

Prostate-specific membrane antigen (PSMA)-targeted radioligands have played an increasing role in the diagnosis of prostate cancer. [Ga]Ga-P16-093 is a PSMA-targeting agent for positron emission tomography imaging, currently under a Phase 2 clinical trial. In the present study, P16-093 was labeled with F via [F]AlF complex formation, and the biological properties of [F]AlF-P16-093 were evaluated. Read More

View Article and Full-Text PDF

Conformational Transition-Triggered Disassembly of Therapeutic Peptide Nanomedicine for Tumor Therapy.

Adv Healthc Mater 2021 Apr 18:e2100333. Epub 2021 Apr 18.

CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), No. 11 Beiyitiao, Zhongguancun, Haidian District, Beijing, 100190, P. R. China.

Cationic therapeutic peptides have received widespread attention due to their excellent antibacterial and antitumor properties. However, most of these peptides have undesirable delivery efficiency and high hemolytic toxicity due to the positively charged α-helix structure containing many lysine and arginine, which may restrict its in vivo applications. Herein, a conformationally transformed therapeutic peptide Pep-HCO modified with bicarbonates on guanidine groups is designed. Read More

View Article and Full-Text PDF

Combination of resveratrol and green tea epigallocatechin gallate induces synergistic apoptosis and inhibits tumor growth in head and neck cancer models.

Oncol Rep 2021 May 13;45(5). Epub 2021 Apr 13.

Department of Hematology and Medical Oncology, Winship Cancer Institute of Emory University, Atlanta, GA 30322, USA.

Despite widespread interest in chemoprevention and therapy due to the high margin of safety of dietary natural compounds, clinical intervention with single agents has failed to yield the expected outcomes, mostly due to poor bioavailability and low potency. Combinations of natural agents with synergistic effects are gaining increasing attention. In the present study, and antitumor effects of a combination of two natural dietary agents, green tea epigallocatechin gallate (EGCG) and resveratrol were investigated. Read More

View Article and Full-Text PDF

Assessment of Phenylboronic Acid Nitrogen Mustards as Potent and Selective Drug Candidates for Triple-Negative Breast Cancer.

ACS Pharmacol Transl Sci 2021 Apr 7;4(2):687-702. Epub 2021 Mar 7.

Department of Chemistry and Biochemistry and the Milwaukee Institute for Drug Discovery, University of Wisconsin-Milwaukee, 3210 N. Cramer Street, Milwaukee, Wisconsin 53211, United States.

Triple-negative breast cancer (TNBC) has limited treatment options and the worst prognosis among all types of breast cancer. We describe two prodrugs, namely, CWB-20145 () and its methyl analogue FAN-NM-CH () that reduced the size of TNBC-derived tumors. The DNA cross-linking of nitrogen mustard prodrugs and was superior to that of chlorambucil and melphalan once activated in the presence of HO. Read More

View Article and Full-Text PDF

Fuzheng Yiliu Formula Regulates Tumor Invasion and Metastasis through Inhibition of WAVE3 Expression.

Evid Based Complement Alternat Med 2021 27;2021:8898668. Epub 2021 Mar 27.

Department of Diagnostics of Chinese Medicine, School of Chinese Medicine, School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.

Objective: To explore the mechanism of action of Fuzheng Yiliu formula (FZYLF) in regulation of the invasion and metastasis of MDA-MB-231/Adr human breast cancer cells through WAVE3.

Methods: The MDA-MB-231/Adr cells with high invasive ability were screened by Transwell, and the plasmid with high WAVE3 expression was made for transfection. Plasmid transfection efficiency and protein expression level were verified by polymerase chain reaction (PCR) and western blotting (WB). Read More

View Article and Full-Text PDF

Virus-Derived Peptides for Hepatic Enzyme Delivery.

Mol Pharm 2021 05 12;18(5):2004-2014. Epub 2021 Apr 12.

Department of Pharmaceutical Sciences, University of Basel, Basel 4056, Switzerland.

Recently, a lipopeptide derived from the hepatitis B virus (HBV) large surface protein has been developed as an HBV entry inhibitor. This lipopeptide, called MyrcludexB (MyrB), selectively binds to the sodium taurocholate cotransporting polypeptide (NTCP) on the basolateral membrane of hepatocytes. Here, the feasibility of coupling therapeutic enzymes to MyrB was investigated for the development of enzyme delivery strategies. Read More

View Article and Full-Text PDF

Dosimetric evaluation of receptor-heterogeneity on the therapeutic efficacy of peptide receptor radionuclide therapy: correlation with DNA damage induction and survival.

J Nucl Med 2021 Apr 9. Epub 2021 Apr 9.

Erasmus MC, Netherlands.

To build a refined dosimetry model for [Lu]Lu-DOTA-[Tyr]octreotate (Lu-DOTATATE) experiments enabling the correlation of absorbed dose with double strand breaks (DSBs) induction and cell death. Somatostatin receptor type-2 (SSTR) expression of NCI-H69 xenografted mice, injected with Lu-DOTATATE, was imaged at 0, 2, 5, 11 days. This was used as input to reconstruct realistic 3 dimensional heterogeneous activity distributions and tissue geometries of both cancer and heathy cells. Read More

View Article and Full-Text PDF

Mild exacerbation of obesity- and age-dependent liver disease progression by senolytic cocktail dasatinib + quercetin.

Cell Commun Signal 2021 Apr 8;19(1):44. Epub 2021 Apr 8.

International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic.

Background: Nonalcoholic fatty liver disease (NAFLD) is increasingly prevalent and represents a growing challenge in terms of prevention and treatment. A minority of affected patients develops inflammation, subsequently fibrosis, cirrhosis and hepatocellular carcinoma (HCC). HCC is a leading cause of cancer-related death. Read More

View Article and Full-Text PDF

Potentiation of the Anticancer Effects by Combining Docetaxel with Ku-0063794 Against Triple-Negative Breast Cancer Cells.

Cancer Res Treat 2021 Apr 5. Epub 2021 Apr 5.

Department of Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Purpose: mTORC1 and mTORC2 inhibition by Ku-0063794 could confer profound anticancer effects against cancer cells because it eliminates feedback activation of Akt. Herein, we aimed to determine anticancer effects of docetaxel and Ku-0063794, individually or in combination, against breast cancer cells, especially triple negative breast cancer (TNBC) cells.

Material And Methods: MCF-7 breast cancer and MDA-MB-231 TNBC cell lines for in vitro studies and mouse xenograft model for in vivo studies were used to investigate the effect of docetaxel, Ku-0063794, or their combination. Read More

View Article and Full-Text PDF

Tubulin acetylation enhances lung cancer resistance to paclitaxel-induced cell death through Mcl-1 stabilization.

Cell Death Discov 2021 Apr 6;7(1):67. Epub 2021 Apr 6.

Preclinical Toxicity and Efficacy Assessment of Medicines and Chemicals Research Cluster, Chulalongkorn University, Bangkok, 10330, Thailand.

The posttranslational modifications (PTMs) of microtubules have been reported to play an important role in cancer aggressiveness, including apoptosis resistance. In this study, we aimed to investigate the biological role of microtubule PTMs in the regulation of paclitaxel responsiveness. The acetylated tubulin (Ace-tub) level was strongly associated with paclitaxel sensitivity, as observed in patient-derived primary lung cancer cells and xenografted immunodeficient mice. Read More

View Article and Full-Text PDF

Preclinical evaluation of anti-VEGFR2 monoclonal antibody ramucirumab labelled with zirconium-89 for tumour imaging.

J Labelled Comp Radiopharm 2021 Apr 5. Epub 2021 Apr 5.

Faculty of Pharmacy in Hradec Kralove, Department of Pharmacology and Toxicology, Charles University, Hradec Kralove, Czech Republic.

The key factors participating in angiogenesis include vascular endothelial growth factor (VEGF) and its receptors (VEGFRs), particularly VEGFR2. Angiogenesis suppression comprises the blocking of the VEGFR2 binding site by the monoclonal antibody ramucirumab (RAM). Our study focused on RAM radiolabelling with zirconium-89 along with subsequent in vitro and in vivo biological evaluation. Read More

View Article and Full-Text PDF

The long non-coding RNA SNHG4/microRNA-let-7e/KDM3A/p21 pathway is involved in the development of non-small cell lung cancer.

Mol Ther Oncolytics 2021 Mar 25;20:634-645. Epub 2020 Dec 25.

Department of Respiratory Medicine, Linyi People's Hospital, Linyi 276000, Shandong Province, P.R. China.

Non-small cell lung cancer (NSCLC) is a foremost cause of malignancy-associated mortality globally. Recent studies have emphasized long non-coding RNAs (lncRNAs) as important biomarkers with diagnostic and therapeutic potential in regard to NSCLC. This study aimed to elucidate the functional role of lncRNA small nucleolar RNA host gene 4 (SNHG4) in NSCLC. Read More

View Article and Full-Text PDF

miR-26a Reverses Multidrug Resistance in Osteosarcoma by Targeting MCL1.

Ming Li Wei Ma

Front Cell Dev Biol 2021 18;9:645381. Epub 2021 Mar 18.

Department of Orthopaedic, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

The multidrug resistance (MDR) acquired in human osteosarcoma is a huge obstacle for effective chemotherapy. Recently, microRNA-26a (miR-26a) has been associated with the pathogenesis and progression of osteosarcoma. However, whether it regulates MDR in osteosarcoma is unknown. Read More

View Article and Full-Text PDF

The Antitumor Peptide ERα17p Exerts Anti-Hyperalgesic and Anti-Inflammatory Actions Through GPER in Mice.

Front Endocrinol (Lausanne) 2021 17;12:578250. Epub 2021 Mar 17.

Université Clermont Auvergne, INSERM, NEURO-DOL Basics & Clinical Pharmacology of Pain, Clermont-Ferrand, France.

Persistent inflammation and persistent pain are major medical, social and economic burdens. As such, related pharmacotherapy needs to be continuously improved. The peptide ERα17p, which originates from a part of the hinge region/AF2 domain of the human estrogen receptor α (ERα), exerts anti-proliferative effects in breast cancer cells through a mechanism involving the hepta-transmembrane G protein-coupled estrogen receptor (GPER). Read More

View Article and Full-Text PDF

Apoptosis Imaging in Oncology by Means of Positron Emission Tomography: A Review.

Int J Mol Sci 2021 Mar 9;22(5). Epub 2021 Mar 9.

Department of Nuclear Medicine AZ Groeninge, 8500 Kortrijk, Belgium.

To date, a wide variety of potential PET-apoptosis imaging radiopharmaceuticals targeting apoptosis-induced cell membrane asymmetry and acidification, as well as caspase 3 activation (substrates and inhibitors) have been developed with the purpose of rapidly assessing the response to treatment in cancer patients. Many of these probes were shown to specifically bind to their apoptotic target in vitro and their uptake to be enhanced in the in vivo-xenografted tumours in mice treated by means of chemotherapy, however, to a significantly variable degree. This may, in part, relate to the tumour model used given the fact that different tumour cell lines bear a different sensitivity to a similar chemotherapeutic agent, to differences in the chemotherapeutic concentration and exposure time, as well as to the different timing of imaging performed post-treatment. Read More

View Article and Full-Text PDF

CD28 Co-Stimulus Achieves Superior CAR T Cell Effector Function against Solid Tumors Than 4-1BB Co-Stimulus.

Cancers (Basel) 2021 Mar 2;13(5). Epub 2021 Mar 2.

Department of Pediatric Oncology and Hematology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 13353 Berlin, Germany.

Spacer or co-stimulatory components in chimeric antigen receptor (CAR) design influence CAR T cell effector function. Few preclinical mouse models optimally support CAR candidate pre-selection for clinical development. Here we use a model in which murine CAR T cells can be exploited with human tumor xenografts. Read More

View Article and Full-Text PDF


Mol Pharmacol 2021 Apr 1. Epub 2021 Apr 1.

Department of Medicine, Division of Medical Oncology, University of Colorado School of Medicine, United States of America

rearrangements occur in 1-2% of lung adenocarcinomas as well as other malignancies and are now established targets for tyrosine kinase inhibitors. We developed three novel -positive() patient-derived cancer cell lines, CUTO22(), CUTO32() and CUTO42() to study RET signaling and response to therapy. We confirmed each of our cell lines express the RET fusion protein and assessed their sensitivity to RET inhibitors. Read More

View Article and Full-Text PDF