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Time from image acquisition to endovascular team notification: a new target for enhancing acute stroke workflow.

J Neurointerv Surg 2021 Apr 8. Epub 2021 Apr 8.

Department of Neurosurgery, Mount Sinai Health System, New York, New York, USA.

Objective: To quantify the time between initial image acquisition (CT angiography (CTA)) and notification of the neuroendovascular surgery (NES) team, a potentially high yield time window to target for optimization of endovascular thrombectomy (ET) treatment times.

Methods: We reviewed our multihospital database for all patients with a stroke with emergent large vessel occlusion treated with ET between January 1, 2017 and August 5, 2020. We dichotomized patients into rapid (≤20 min) and delayed (>20 min) notification times and analyzed treatment characteristics and outcomes. Read More

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Transcriptional changes through menstrual cycle reveal a global transcriptional derepression underlying the molecular mechanism involved in the window of implantation.

Mol Hum Reprod 2021 Apr 8. Epub 2021 Apr 8.

Department of Genomic & Systems Reproductive Medicine, IVI-RMA IVI Foundation - Instituto de Investigación Sanitaria Hospital Universitario y Politécnico La Fe, Av. Fernando Abril Martorell 106, Torre A, Planta 1a, 46026 Valencia, Spain.

The human endometrium is a dynamic tissue that only is receptive to host the embryo during a brief time in the middle secretory phase, called the window of implantation (WOI). Despite its importance, regulation of the menstrual cycle remains incompletely understood. The aim of this study was to characterize the gene cooperation and regulation of menstrual cycle progression, in order to dissect the molecular complexity underlying acquisition of endometrial receptivity for a successful pregnancy; and to provide the scientific community with detailed gene co-expression information throughout the menstrual cycle on a user-friendly web-tool database. Read More

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DYNAMIC FUNCTIONAL CONNECTIVITY IN THE MAIN CLINICAL PHENOTYPES OF MULTIPLE SCLEROSIS.

Brain Connect 2021 Apr 3. Epub 2021 Apr 3.

IRCCS San Raffaele Scientific Institute, Nuerology Unit, via Olgettina 60, Milano, Italy, 20132.

Introduction: Dynamic functional connectivity (dFC) allows capturing recurring patterns (states) of interaction among functional networks. Here, we investigated resting state (RS) dFC abnormalities across the different clinical phenotypes of multiple sclerosis (MS) and assessed their correlation with motor and cognitive performances.

Methods: RS fMRI and 3D T1-weighted MRI data were acquired from 128 MS patients (69 relapsing-remitting [RR] MS, 34 secondary progressive [SP] MS, 25 primary progressive [PP] MS) and 40 healthy controls (HC). Read More

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Media exposure and language experience: Examining associations from home observations in Mexican immigrant families in the US.

Infant Behav Dev 2021 Mar 31;63:101554. Epub 2021 Mar 31.

Communication Sciences and Disorders, Department of Special Education and Clinical Sciences, University of Oregon, United States.

The current exploratory study describes exposure to digital media in young children from Mexican immigrant homes and its association with language input and output. Using multiple recordings of children's home environments, we report on the rate (i.e. Read More

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Modulating the Heat Stress Response to Improve Hyperthermia-Based Anticancer Treatments.

Cancers (Basel) 2021 Mar 12;13(6). Epub 2021 Mar 12.

Department of Medical Biology, Amsterdam University Medical Centers, University of Amsterdam, Cancer Center Amsterdam, Meibergdreef 9, 1105AZ Amsterdam, The Netherlands.

Cancer treatments based on mild hyperthermia (39-43 °C, HT) are applied to a widening range of cancer types, but several factors limit their efficacy and slow down more widespread adoption. These factors include difficulties in adequate heat delivery, a short therapeutic window and the acquisition of thermotolerance by cancer cells. Here, we explore the biological effects of HT, the cellular responses to these effects and their clinically-relevant consequences. Read More

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Discovery of Screening Biomarkers for Major Depressive Disorder in Remission by Proteomic Approach.

Diagnostics (Basel) 2021 Mar 17;11(3). Epub 2021 Mar 17.

Department of Biomedical Laboratory Science, College of Health Science, Eulji University, Uijeongbu 11759, Korea.

Major depressive disorder (MDD) is a common disorder involving depressive mood and decreased motivation. Due to its high heterogeneity, novel biomarkers are required to diagnose MDD. In this study, a proteomic method was used to identify a new MDD biomarker. Read More

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Neurophysiological and functional neuroanatomical coding of statistical and deterministic rule information during sequence learning.

Hum Brain Mapp 2021 Apr 2. Epub 2021 Apr 2.

Institute of Psychology, ELTE Eötvös Loránd University, Budapest, Hungary.

Humans are capable of acquiring multiple types of information presented in the same information stream. It has been suggested that at least two parallel learning processes are important during learning of sequential patterns-statistical learning and rule-based learning. Yet, the neurophysiological underpinnings of these parallel learning processes are not fully understood. Read More

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Optimizing Identification of Power Injectable Ports on the Scout Images for Multidetector Computed Tomography Procedures.

J Comput Assist Tomogr 2021 Mar 12. Epub 2021 Mar 12.

From the Department of Radiology, Duke University Medical Center Department of Radiology, Duke University Medical Physics Graduate Program, Durham, NC.

Objective: The objective of this study was to assess the impact of tube voltage and image display on the identification of power ports features on anterior-posterior scout images to inform optimal workflow for multidetector computed tomography (MDCT) examinations.

Materials And Methods: Four ports, representing variable material composition (titanium/silicone), shapes, and computed tomography (CT) markings, were imaged on an adult anthropomorphic chest phantom using a dual-source MDCT at variable peak tube voltages (80, 100, 120, 150, and Sn150 kVp). Images were reviewed at variable image display setting by 5 blinded readers to assess port features of material composition, shape, and text markings as well as overall preferred image quality. Read More

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Patient-derived organoids and high grade serous ovarian cancer: from disease modeling to personalized medicine.

J Exp Clin Cancer Res 2021 Mar 31;40(1):116. Epub 2021 Mar 31.

Dipartimento di Neuroscienze, Sezione di Anatomia Umana, Università Cattolica del Sacro Cuore, Roma, Italy.

Background: High grade serous ovarian cancer (HGSOC) is among the deadliest human cancers and its prognosis remains extremely poor. Tumor heterogeneity and rapid acquisition of resistance to conventional chemotherapeutic approaches strongly contribute to poor outcome of patients. The clinical landscape of HGSOC has been radically transformed since the advent of targeted therapies in the last decade. Read More

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Ultra-fast proteomics with Scanning SWATH.

Nat Biotechnol 2021 Mar 25. Epub 2021 Mar 25.

Molecular Biology of Metabolism Laboratory, The Francis Crick Institute, London, UK.

Accurate quantification of the proteome remains challenging for large sample series and longitudinal experiments. We report a data-independent acquisition method, Scanning SWATH, that accelerates mass spectrometric (MS) duty cycles, yielding quantitative proteomes in combination with short gradients and high-flow (800 µl min) chromatography. Exploiting a continuous movement of the precursor isolation window to assign precursor masses to tandem mass spectrometry (MS/MS) fragment traces, Scanning SWATH increases precursor identifications by ~70% compared to conventional data-independent acquisition (DIA) methods on 0. Read More

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Honey proteomic signatures for the identification of honey adulterated with syrup, producing country, and nectar source using SWATH-MS approach.

Food Chem 2021 Mar 15;354:129590. Epub 2021 Mar 15.

Institute of Molecular Biology, National Chung Hsing University, Taichung 40227, Taiwan; Graduate Institute of Chinese Medical Science, China Medical University, Taichung 40447, Taiwan; Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan; Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung 40227, Taiwan; Rong Hsing Research Center For Translational Medicine, National Chung Hsing University, Taichung 40227, Taiwan. Electronic address:

Honey is widely consumed by humans, due to its multiple applications as a food constituent and its therapeutic effects. This study reports on the discrimination of honey products from different geographical and botanical sources, as well as honey products containing distinct forms of syrup used in honey adulteration. Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS)-based proteomic analysis combined with chemometrics was successfully applied in identifying characteristic proteins that can be used as biomarkers of the original source of honey. Read More

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Inhibition of PACAP/PAC1/VPAC2 signaling impairs the consolidation of social recognition memory and nitric oxide prevents this deficit.

Neurobiol Learn Mem 2021 Mar 9;180:107423. Epub 2021 Mar 9.

Memory Center, Brain Institute of Rio Grande do Sul, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Av. Ipiranga, 6690-2nd Floor, 90610-000 Porto Alegre, RS, Brazil; National Institute of Translational Neuroscience (INNT), National Research Council of Brazil, Federal University of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil. Electronic address:

Social recognition memory (SRM) forms the basis of social relationships of animals. It is essential for social interaction and adaptive behavior, reproduction and species survival. Evidence demonstrates that social deficits of psychiatric disorders such as autism and schizophrenia are caused by alterations in SRM processing by the hippocampus and amygdala. Read More

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Discovery and Identification of Arsenolipids Using a Precursor-Finder Strategy and Data-Independent Mass Spectrometry.

Environ Sci Technol 2021 Mar 5;55(6):3836-3844. Epub 2021 Mar 5.

Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, 10-102 Clinical Sciences Building, Edmonton, Alberta T6G 2G3, Canada.

Arsenolipids are a class of lipid-soluble arsenic species. They are present in seafoods and show high potentials of cytotoxicity and neurotoxicity. Hindered by traditional low-throughput analytical techniques, the characterization of arsenolipids is far from complete. Read More

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Toward a new model for speech perception: the Universal Perceptual Model (UPM) of second language.

Cogn Process 2021 Feb 16. Epub 2021 Feb 16.

Department of Languages and Literature, University of Nicosia, Nicosia, Cyprus.

Several speech models have been formed in the past aiming to predict the abilities of nonnative listeners or learners in perceiving and producing speech sounds. The present paper proposes a new model for speech perception, the Universal Perceptual Model of Second Language (henceforth, UPM). UPM assumes that second language phone acquisition is strongly affected by the speakers' native language but still the window of phone learning is open due to the universality of speech sounds. Read More

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February 2021

Minimizing ion competition boosts volatile metabolome coverage by secondary electrospray ionization orbitrap mass spectrometry.

Anal Chim Acta 2021 Mar 16;1150:338209. Epub 2021 Jan 16.

Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093, Zurich, Switzerland. Electronic address:

Secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) is an emerging technique for the detection of volatile metabolites. However, sensitivity and reproducibility of SESI-HRMS have limited its applications in untargeted metabolomics profiling. Ion suppression in the SESI source has been considered to be the main cause. Read More

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Identification of differential protein expression and putative drug target in metacyclic stage of Leishmania major and Leishmania tropica: A quantitative proteomics and computational view.

Comp Immunol Microbiol Infect Dis 2021 Apr 27;75:101617. Epub 2021 Jan 27.

Department of Clinical Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Cutaneous leishmaniasis (CL) is an infectious disease that commonly caused by Leishmania (L.) major and L.tropica. Read More

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Mechanism behind the neuronal ephaptic coupling during synchronizing by specific brain-triggered wave as neuronal motor toolkit.

Sci Rep 2021 Feb 11;11(1):3683. Epub 2021 Feb 11.

Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Probable mechanism behind the neuronal ephaptic coupling is investigated based on the introduction of "Brain"-triggered potential excitation signal smartly with a specific very low frequency (VLF) waves as a neuronal motor toolkit. Detection of this electric motor toolkit is attributed to in-vitro precise analyses of a neural network of snail, along to the disconnected snail's neuronal network as a control. This is achieved via rapid (real-time) electrical signals acquisition by blind patch-clamp method during micro-electrode implanting in the neurons at the gigaseal conditions by the surgery operations. Read More

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February 2021

Computed Tomography-Based Measurements of the Cochlear Duct: Implications for Cochlear Implant Pitch Tuning.

Ear Hear 2021 Feb 2. Epub 2021 Feb 2.

Department of Otolaryngology-Head and Neck Surgery, University of California San Francisco School of Medicine, San Francisco, California School of Medicine, Johns Hopkins University, Baltimore, Maryland These authors contributed equally to this work.

Objectives: To determine the sources of variability for cochlear duct length (CDL) measurements for the purposes of fine-tuning cochlear implants (CI) and to propose a set of standardized landmarks for computed tomography (CT) pitch mapping.

Design: This was a retrospective cohort study involving 21 CI users at a tertiary referral center. The intervention involved flat-panel CT image acquisition and secondary reconstructions of CIs in vivo. Read More

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February 2021

Conditional knockout of MET receptor tyrosine kinase in cortical excitatory neurons leads to enhanced learning and memory in young adult mice but early cognitive decline in older adult mice.

Neurobiol Learn Mem 2021 03 29;179:107397. Epub 2021 Jan 29.

Basic Medical Sciences, University of Arizona College of Medicine-Phoenix, Phoenix, AZ 85004, United States. Electronic address:

Human genetic studies established MET gene as a risk factor for autism spectrum disorders. We have previously shown that signaling mediated by MET receptor tyrosine kinase, expressed in early postnatal developing forebrain circuits, controls glutamatergic neuron morphological development, synapse maturation, and cortical critical period plasticity. Here we investigated how MET signaling affects synaptic plasticity, learning and memory behavior, and whether these effects are age-dependent. Read More

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Observation of photon antibunching with only one standard single-photon detector.

Rev Sci Instrum 2021 Jan;92(1):013105

College of Information Science and Electronic Engineering, Zhejiang University, Hangzhou 310027, China.

The second-order photon correlation function g(τ) is of great importance in quantum optics. g(τ) is typically measured with the Hanbury Brown and Twiss (HBT) interferometer, which employs a pair of single-photon detectors and a dual-channel time acquisition module. Here, we demonstrate a new method to measure and extract g(τ) with a standard single-photon avalanche photodiode (dead-time = 22 ns) and a single-channel time acquisition module. Read More

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January 2021

Corrole-Substituted Fluorescent Heme Proteins.

Inorg Chem 2021 Feb 29;60(4):2716-2729. Epub 2021 Jan 29.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, United States.

Although fluorescent proteins have been utilized for a variety of biological applications, they have several optical limitations, namely weak red and near-infrared emission and exceptionally broad (>200 nm) emission profiles. The photophysical properties of fluorescent proteins can be enhanced through the incorporation of novel cofactors with the desired properties into a stable protein scaffold. To this end, a fluorescent phosphorus corrole that is structurally similar to the native heme cofactor is incorporated into two exceptionally stable heme proteins: H-NOX from and heme acquisition system protein A (HasA) from . Read More

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February 2021

An improved window of interest for electroanatomical mapping of atrial tachycardia.

J Interv Card Electrophysiol 2021 Jan 27. Epub 2021 Jan 27.

Ramsay Santé, Hôpital Privé Clairval, Service Cardiologie-Rythmologie, Marseille, France.

Purpose: Diagnosis of atrial tachycardia (AT) with 3D mapping system remains challenging due to fibrosis or previous ablation. This study aims to evaluate a new electroanatomical mapping annotation setting using a window of interest adjusted at the end of the P wave (WOI) to identify the AT mechanism more accurately.

Methods: Twenty patients with successful ablation of left AT using navigation system CARTO3 were evaluated. Read More

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January 2021

Impaired prenatal motor axon development necessitates early therapeutic intervention in severe SMA.

Sci Transl Med 2021 Jan;13(578)

Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Gene replacement and pre-mRNA splicing modifier therapies represent breakthrough gene targeting treatments for the neuromuscular disease spinal muscular atrophy (SMA), but mechanisms underlying variable efficacy of treatment are incompletely understood. Our examination of severe infantile onset human SMA tissues obtained at expedited autopsy revealed persistence of developmentally immature motor neuron axons, many of which are actively degenerating. We identified similar features in a mouse model of severe SMA, in which impaired radial growth and Schwann cell ensheathment of motor axons began during embryogenesis and resulted in reduced acquisition of myelinated axons that impeded motor axon function neonatally. Read More

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January 2021

Dynamic Alteration in the Vaginal Secretory Proteome across the Early and Mid-Trimesters of Pregnancy.

J Proteome Res 2021 Feb 26;20(2):1190-1205. Epub 2021 Jan 26.

Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad 121001, India.

Pregnancy is characterized by intense physiological and structural alterations in the vagina, cervix, and overlying fetal membranes. High vaginal fluid (HVF) is a proximal fluid that covers the lower part of the female reproductive system and the severity of vaginal pathology often adversely affects pregnancy outcomes. To identify the correlation of vaginal fluid proteome dynamics and physiological changes during the progression of pregnancy, a longitudinal study was performed on 20 pregnant women who delivered a baby in >37 weeks without any complications. Read More

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February 2021

Effect of Shortening the Scan Duration on Quantitative Accuracy of [F]Flortaucipir Studies.

Mol Imaging Biol 2021 Jan 26. Epub 2021 Jan 26.

Department of Radiology & Nuclear Medicine, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam UMC, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.

Purpose: Dynamic positron emission tomography (PET) protocols allow for accurate quantification of [F]flortaucipir-specific binding. However, dynamic acquisitions can be challenging given the long required scan duration of 130 min. The current study assessed the effect of shorter scan protocols for [F]flortaucipir on its quantitative accuracy. Read More

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January 2021

Pilus Production in Acinetobacter baumannii Is Growth Phase Dependent and Essential for Natural Transformation.

J Bacteriol 2021 Mar 23;203(8). Epub 2021 Mar 23.

Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

is a severe threat to human health as a frequently multidrug-resistant hospital-acquired pathogen. Part of the danger from this bacterium comes from its genome plasticity and ability to evolve quickly by taking up and recombining external DNA into its own genome in a process called natural competence for transformation. This mode of horizontal gene transfer is one of the major ways that bacteria can acquire new antimicrobial resistances and toxic traits. Read More

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High-throughput, in-depth and estimated absolute quantification of plasma proteome using data-independent acquisition/mass spectrometry ("HIAP-DIA").

Proteomics 2021 Mar 23;21(5):e2000264. Epub 2021 Feb 23.

The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.

Mass spectrometry-based plasma proteomics has been demonstrated to be a useful tool capable of quantifying hundreds of proteins in a single LC-MS/MS experiment, for biomarker discovery or elucidation of disease mechanisms. We developed a novel data-independent acquisition (DIA)/MS-based workflow for high-throughput, in-depth and estimated absolute quantification of plasma proteins (termed HIAP-DIA), without depleting high-abundant proteins, in a single-shot experiment. In HIAP-DIA workflow, we generated an ultra-deep cumulative undepleted and depleted spectral library which contained 55,157 peptides and 5,328 proteins, optimized column length (50 cm) and gradient (90 min) of liquid chromatography instrumentation, optimized 50 DIA segments with average isolation window 17 Th, and selected reference proteins for estimated absolute quantification of all plasma proteins. Read More

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Chronic pain susceptibility is associated with anhedonic behavior and alterations in the accumbal ubiquitin-proteasome system.

Pain 2021 01 12. Epub 2021 Jan 12.

Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal. ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Portugal. Faculty of Medicine, University of Coimbra, Portugal.

Abstract: It remains unknown why upon similar acute/subacute painful conditions, pain persists in some individuals while in others it resolves. Genetic factors, mood, and functional alterations, particularly involving the mesolimbic network, appear to be key. In order to explore potential susceptibility/resistance factors, we screened a large population of rats with a peripheral neuropathy and we isolated a small subset (<15%) that presented high thresholds (HT) to mechanical allodynia (reduced pain manifestation). Read More

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January 2021

Early β adrenoceptor dependent time window for fear memory persistence in APPswe/PS1dE9 mice.

Sci Rep 2021 Jan 13;11(1):870. Epub 2021 Jan 13.

Centre for Brain Research, SID Complex, Indian Institute of Science, Bangalore, 560 012, India.

In this study we demonstrate that 2 month old APPswe/PS1dE9 mice, a transgenic model of Alzheimer's disease, exhibited intact short-term memory in Pavlovian hippocampal-dependent contextual fear learning task. However, their long-term memory was impaired. Intra-CA1 infusion of isoproterenol hydrochloride, the β-adrenoceptor agonist, to the ventral hippocampus of APPswe/PS1dE9 mice immediately before fear conditioning restored long-term contextual fear memory. Read More

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January 2021

Time-course study of a gold nanoparticle contrast agent for cardiac-gated micro-CT imaging in mice.

Biomed Phys Eng Express 2020 04 15;6(3):035025. Epub 2020 Apr 15.

Oral Biological and Medical Sciences, The University of British Columbia, Vancouver, Canada. Université Grenobles Alpes, Grenoble, France.

Although micro-computed tomography (micro-CT) images have high contrast for bone or air, between soft tissues the contrast is typically low. To overcome this inherent issue, attenuating exogenous contrast agents are used to provide contrast enhancement in the vasculature and abdominal organs. The aim of this study is to measure the contrast enhancement time course for a gold nanoparticle blood-pool contrast agent and use it to perform cardiac-gated 4D micro-CT scans of the heart. Read More

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