42,771 results match your criteria wild strain


Cantil: a previously unreported organ in wild-type Arabidopsis regulated by FT, ERECTA and heterotrimeric G proteins.

Development 2021 Jun 15;148(11). Epub 2021 Jun 15.

Department of Biology, The Pennsylvania State University, University Park, PA 16802, USA.

We describe a previously unreported macroscopic Arabidopsis organ, the cantil, named for its 'cantilever' function of holding the pedicel at a distance from the stem. Cantil development is strongest at the first nodes after the vegetative to reproductive inflorescence transition; cantil magnitude and frequency decrease acropetally. Cantils develop in wild-type Arabidopsis accessions (e. Read More

View Article and Full-Text PDF

Attenuated strain of CVB3 with a mutation in the CAR-interacting region protects against both myocarditis and pancreatitis.

Sci Rep 2021 Jun 14;11(1):12432. Epub 2021 Jun 14.

School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.

Coxsackievirus B3 (CVB3), is commonly implicated in myocarditis, which can lead to dilated cardiomyopathy, in addition to causing acute pancreatitis and meningitis. Yet, no vaccines are currently available to prevent this infection. Here, we describe the derivation of a live attenuated vaccine virus, termed mutant (Mt) 10, encoding a single amino acid substitution H790A within the viral protein 1, that prevents CVB3 infection in mice and protects from both myocarditis and pancreatitis in challenge studies. Read More

View Article and Full-Text PDF

RNA and quantitative proteomic analysis of Dictyostelium knock-out cells lacking the core autophagy proteins ATG9 and/or ATG16.

BMC Genomics 2021 Jun 15;22(1):444. Epub 2021 Jun 15.

Center for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Str. 52, 50931, Cologne, Germany.

Background: Autophagy is an evolutionary ancient mechanism that sequesters substrates for degradation within autolysosomes. The process is driven by many autophagy-related (ATG) proteins, including the core members ATG9 and ATG16. However, the functions of these two core ATG proteins still need further elucidation. Read More

View Article and Full-Text PDF

A Genetic Titration of Membrane Composition in C. elegans Reveals its Importance for Multiple Cellular and Physiological Traits.

Genetics 2021 Jun 14. Epub 2021 Jun 14.

Dept.Chemistry and Molecular Biology, Univ. Gothenburg, 405 30 Gothenburg, Sweden.

The composition and biophysical properties of cellular membranes must be tightly regulated to maintain the proper functions of myriad processes within cells. To better understand the importance of membrane homeostasis, we assembled a panel of five C. elegans strains that show a wide span of membrane composition and properties, ranging from excessively rich in saturated fatty acids (SFAs) and rigid to excessively rich in polyunsaturated fatty acids (PUFAs) and fluid. Read More

View Article and Full-Text PDF

Conservative production of galactosaminogalactan in Metarhizium is responsible for appressorium mucilage production and topical infection of insect hosts.

PLoS Pathog 2021 Jun 14;17(6):e1009656. Epub 2021 Jun 14.

CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.

The exopolysaccharide galactosaminogalactan (GAG) has been well characterized in Aspergilli, especially the human pathogen Aspergillus fumigatus. It has been found that a five-gene cluster is responsible for GAG biosynthesis in Aspergilli to mediate fungal adherence, biofilm formation, immunosuppression or induction of host immune defences. Herein, we report the presence of the conserved GAG biosynthetic gene cluster in the insect pathogenic fungus Metarhizium robertsii to mediate either similar or unique biological functions. Read More

View Article and Full-Text PDF

A capsule-associated gene of , , is involved in pH homeostasis.

Microbiology (Reading) 2021 Jun;167(6)

Department of Biological Science and Technology, Tokyo University of Science, Niijuku 6-3-1, Katsushika-ku, Tokyo 125-8585, Japan.

The gene is known to be involved in capsule formation in the basidiomycete yeast . A null mutant of , Δ, lacks a capsule around the cell wall and its acidic organelles are not stained with quinacrine. In order to clarify whether the Cap64 protein indeed maintains vacuole or vesicle acidification, so that the vesicle containing the capsule polysaccharide or DBB substrate are transported to the cell membrane side, the relationship between and intracellular transport genes and between and enzyme-secretion activity were analysed. Read More

View Article and Full-Text PDF

Identification of novel mutations contributing to azole tolerance of through exposure to tebuconazole.

Antimicrob Agents Chemother 2021 Jun 14:AAC0265720. Epub 2021 Jun 14.

Medical Mycology Research Center, Chiba University, Chiba, Chiba, Japan.

Azole resistance of is a global problem. The major resistant mechanism is a cytochrome P 14-α sterol demethylase Cyp51A alteration such as mutation(s) in the gene and the acquisition of a tandem repeat in the promoter. Although other azole tolerances and resistant mechanisms such as (a 3-hydroxy-3-methylglutaryl-coenzyme-A reductase gene) mutation are known, few reports have described studies elucidating non-Cyp51A resistance mechanisms. Read More

View Article and Full-Text PDF

Topoisomerase I Essentiality, DnaA-independent Chromosomal Replication, and Transcription-Replication Conflict in .

J Bacteriol 2021 Jun 14:JB0019521. Epub 2021 Jun 14.

Laboratory of Bacterial Genetics, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India.

Topoisomerase I (Topo I) of , encoded by , acts to relax negative supercoils in DNA. Topo I deficiency results in hypernegative supercoiling, formation of transcription-associated RNA-DNA hybrids (R-loops), and DnaA- and -independent constitutive stable DNA replication (cSDR), but some uncertainty persists as to whether is essential for viability in and related enterobacteria. Here we show that several alleles, including Δ, confer lethality in derivatives of wild-type strain MG1655. Read More

View Article and Full-Text PDF

Functional insights into the high-molecular-mass penicillin-binding proteins of revealed by gene deletion and transposon mutagenesis analysis.

J Bacteriol 2021 Jun 14:JB0023421. Epub 2021 Jun 14.

Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Research Institute, and Department of Pathology and Genomic Medicine, Houston Methodist Hospital, Houston, Texas, USA.

High-molecular-mass penicillin-binding proteins (PBPs) are enzymes that catalyze the biosynthesis of bacterial cell wall peptidoglycan. The Gram-positive bacterial pathogen (group B , or GBS) produces five high-molecular-mass PBPs, namely, PBP1A, PBP1B, PBP2A, PBP2B, and PBP2X. Among these, only PBP2X is essential for cell viability, whereas the other four PBPs are individually dispensable. Read More

View Article and Full-Text PDF

Substitution of a Surface-Exposed Residue Involved in an Allosteric Network Enhances Tryptophan Synthase Function in Cells.

Front Mol Biosci 2021 26;8:679915. Epub 2021 May 26.

Department of Chemistry, The Pennsylvania State University, University Park, PA, United States.

Networks of noncovalent amino acid interactions propagate allosteric signals throughout proteins. Tryptophan synthase (TS) is an allosterically controlled bienzyme in which the indole product of the alpha subunit (αTS) is transferred through a 25 Å hydrophobic tunnel to the active site of the beta subunit (βTS). Previous nuclear magnetic resonance and molecular dynamics simulations identified allosteric networks in αTS important for its function. Read More

View Article and Full-Text PDF

Genotypic Males Play an Important Role in the Creation of Genetic Diversity in Gynogenetic Gibel Carp.

Front Genet 2021 28;12:691923. Epub 2021 May 28.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovative Academy of Seed Design, Hubei Hongshan Laboratory, Chinese Academy of Sciences, Wuhan, China.

Unisexual lineages are commonly considered to be short-lived in the evolutionary process as accumulation of deleterious mutations stated by Muller's ratchet. However, the gynogenetic hexaploid gibel carp () with existence over 0.5 million years has wider ecological distribution and higher genetic diversity than its sexual progenitors, which provides an ideal model to investigate the underlying mechanisms on countering Muller's ratchet in unisexual taxa. Read More

View Article and Full-Text PDF

Brucella Infection Investigation in Cetaceans and Manatees in Northeast Brazil.

J Aquat Anim Health 2021 Jun 14. Epub 2021 Jun 14.

Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, São Paulo, 13635-900, Brazil.

Among the bacterial infections that impair the health status of marine mammals, those caused by Brucella spp. are the most reported worldwide. Brucella infections in marine mammals can result in acute or chronic disease and are associated with variable clinical outcomes, depending on the organ involved during the infectious process, infection route, host immunity, and strain pathogenicity. Read More

View Article and Full-Text PDF

Potent RBD-specific neutralizing rabbit monoclonal antibodies recognize emerging SARS-CoV-2 variants elicited by DNA prime-protein boost vaccination.

Emerg Microbes Infect 2021 Jun 14:1-37. Epub 2021 Jun 14.

Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, Jiangsu, China.

Global concerns arose as the emerged and rapidly spreading SARS-CoV-2 variants might escape host immunity induced by vaccination. In this study, a heterologous prime-boost immunization strategy for COVID-19 was designed to prime with a DNA vaccine encoding wild type (WT) spike protein receptor-binding domain (RBD) followed by S1 protein-based vaccine in rabbits. Four vaccine-elicited rabbit monoclonal antibodies (RmAbs), including 1H1, 9H1, 7G5, and 5E1, were isolated for biophysical property, neutralization potency and sequence analysis. Read More

View Article and Full-Text PDF

Functional characterization of knockdown resistance mutations in the plant bug, Apolygus lucorum Meyer-Dür.

Pestic Biochem Physiol 2021 Jul 20;176:104874. Epub 2021 May 20.

College of Plant Protection, Hainan University, Haikou 570228, China. Electronic address:

Apolygus lucorum could cause severe economic damage to crops in China. The pest has been controlled by pyrethroids, and the target of pyrethroids is voltage-gated sodium channel (Na). Double mutation (LF/DG) was detected in a field-strain of A. Read More

View Article and Full-Text PDF

Application of MALDI-TOF analysis to reveal diversity and dynamics of winemaking yeast species in wild-fermented, organically produced, New Zealand Pinot Noir wine.

Food Microbiol 2021 Oct 17;99:103824. Epub 2021 May 17.

Department of Wine, Food and Molecular Biosciences, Lincoln University, PO Box 85054, Lincoln, New Zealand. Electronic address:

Rapid yeast identification is of particular importance in monitoring wine fermentation and assessing strain application in winemaking. We used MALDI-TOF MS analysis supported by 26 S rRNA gene sequence analysis and Saccharomyces-specific PCR testing to differentiate reference and field strains recovered from organic wine production facilities in Waipara, New Zealand, in which Pinot Noir wine was produced by spontaneous fermentations in the vineyard and in the winery. Strains were isolated from each of four key stages of each ferment to evaluate changes in taxonomic diversity. Read More

View Article and Full-Text PDF
October 2021

An inside look at a biofilm: flagella biotracking.

Sci Adv 2021 Jun 11;7(24). Epub 2021 Jun 11.

Department of Life Sciences, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 8410501, Israel.

The opportunistic pathogen, , a flagellated bacterium, is one of the top model organisms for biofilm studies. To elucidate the location of bacterial flagella throughout the biofilm life cycle, we developed a new flagella biotracking tool. Bacterial flagella were site-specifically labeled via genetic code expansion. Read More

View Article and Full-Text PDF

Identification of a lactose-oxidizing enzyme in Escherichia coli and improvement of lactobionic acid production by recombinant expression of a quinoprotein glucose dehydrogenase from Pseudomonas taetrolens.

Enzyme Microb Technol 2021 Aug 14;148:109828. Epub 2021 May 14.

Bio-based Chemistry Research Center, Korea Research Institute of Chemical Technology (KRICT), 406-30, Jongga-ro, Ulsan, 44429, Republic of Korea; Advanced Materials and Chemical Engineering, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea. Electronic address:

Lactobionic acid (LBA), an aldonic acid prepared by oxidation of the free aldehyde group of lactose, has been broadly used in cosmetic, food, and pharmaceutical industries. Although Escherichia coli is unable to produce LBA naturally, a wild-type E. coli strain successfully produced LBA from lactose upon pyrroloquinoline quinone (PQQ) supplementation, indicating that E. Read More

View Article and Full-Text PDF

Immunization of chicken with flagellin adjuvanted Salmonella enteritidis bacterial ghosts confers complete protection against chicken salmonellosis.

Poult Sci 2021 Apr 21;100(7):101205. Epub 2021 Apr 21.

College of Veterinary Medicine, Jeonbuk National University, Iksan Campus, 54596, Iksan, Republic of Korea. Electronic address:

The present study describes the generation of Salmonella enteritidis (SE) ghosts with a surface decorated Salmonella Typhimurium (ST) flagellin (FliC) antigen for immune enhancement and strain-specific protection. The ghosts were generated by biological means using pJHL184::fliC temperature inducible plasmid where the lysis occurs by phage PhiX174 lysis gene E expression. Being an inactivated strain, no environmental contamination was observed by fecal shedding upon inoculation into the chicken. Read More

View Article and Full-Text PDF

Function and distribution of the conjugative plasmid pLM1686 in foodborne Listeria monocytogenes in China.

Int J Food Microbiol 2021 May 30;352:109261. Epub 2021 May 30.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Institute of Pathogenic Biology, Hengyang Medical College, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang 421001, China. Electronic address:

Listeria monocytogenes, a fatal foodborne pathogen has the extraordinary capacity to survive in harsh conditions and is a potential threat to public health. A novel 91 kb plasmid pLM1686 was found in the prevalent L. monocytogenes sequence type (ST) 87 strain in China. Read More

View Article and Full-Text PDF

Rapid detection of multidrug-resistant tuberculosis based on allele-specific recombinase polymerase amplification and colorimetric detection.

PLoS One 2021 11;16(6):e0253235. Epub 2021 Jun 11.

Research Unit of Innovative Diagnosis of Antimicrobial Resistance, Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.

Multidrug-resistant tuberculosis (MDR-TB) poses a serious threat to TB control. Early diagnosis and proper treatment are essential factors to limit the spread of the disease. The existing molecular tests for MDR-TB usually require specific instruments, steady power supply, and routine maintenance, which might be obstacles for low-resource settings. Read More

View Article and Full-Text PDF

Emerging mutant populations of Listeria monocytogenes EGD-e under selective pressure of Thymbra capitata essential oil question its use in food preservation.

Food Res Int 2021 Jul 12;145:110403. Epub 2021 May 12.

Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain. Electronic address:

Due to their excellent antimicrobial properties, essential oils (EO) have been proposed as potential preservatives for certain kinds of foods, such as dairy products. However, the occurrence of pathogenic populations that are resistant to EOs could pose a health risk. This report seeks to assess the emergence of resistant populations in Listeria monocytogenes EGD-e growth at 37 °C under selective pressure of Thymbra capitata EO (TCO), to characterise their resistance in laboratory media, and to identify their genotypic changes, as well as to evaluate the resistance in skimmed milk. Read More

View Article and Full-Text PDF

Role of the VirSR-VirAB system in biofilm formation of Listeria monocytogenes EGD-e.

Food Res Int 2021 Jul 8;145:110394. Epub 2021 May 8.

College of Chemistry and Materials Engineering, Xinxiang University, Xinxiang, China.

The ability of Listeria monocytogenes, an important foodborne pathogen, to form biofilms in food processing environments leads to increased opportunity for contamination of food products, which is a major concern for food safety. In this study, the role of a complex system composed of the VirSR two-component signal transduction system (TCS) and the ATP-binding cassette (ABC) transporter VirAB in biofilm formation of L. monocytogenes EGD-e was investigated. Read More

View Article and Full-Text PDF

Generation of anisotropic strain dysregulates wild-type cell division at the interface between host and oncogenic tissue.

Curr Biol 2021 Jun 3. Epub 2021 Jun 3.

Wellcome Trust Centre for Cell-Matrix Research, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine & Health, Manchester Academic Health Science Centre, University of Manchester, Oxford Road, Manchester M13 9PT, UK. Electronic address:

Epithelial tissues are highly sensitive to anisotropies in mechanical force, with cells altering fundamental behaviors, such as cell adhesion, migration, and cell division. It is well known that, in the later stages of carcinoma (epithelial cancer), the presence of tumors alters the mechanical properties of a host tissue and that these changes contribute to disease progression. However, in the earliest stages of carcinoma, when a clonal cluster of oncogene-expressing cells first establishes in the epithelium, the extent to which mechanical changes alter cell behavior in the tissue as a whole remains unclear. Read More

View Article and Full-Text PDF

Role of qGZn9a in controlling grain zinc concentration in rice, Oryza sativa L.

Theor Appl Genet 2021 Jun 10. Epub 2021 Jun 10.

Laboratory of Plant Breeding, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan.

Key Message: A candidate gene responsible for higher grain zinc accumulation in rice was identified, which was probably associated with a partial defect in anther dehiscence. Zinc (Zn) is an essential mineral element in many organisms. Zn deficiency in humans causes various health problems; therefore, an adequate dietary Zn intake is required daily. Read More

View Article and Full-Text PDF

Complete Genome Sequence of Neisseria musculi Using Illumina and PacBio Sequencing.

Microbiol Resour Announc 2021 Jun 10;10(23):e0045221. Epub 2021 Jun 10.

Department of Biological Sciences, Ohio University, Athens, Ohio, USA.

Neisseria musculi is an oral commensal of wild-caught mice. Here, we report the complete genome sequence of strain NW831, generated using a combination of the Illumina and PacBio platforms. Read More

View Article and Full-Text PDF

Efficacy of Wolbachia-Infected Mosquito Deployments for the Control of Dengue.

N Engl J Med 2021 06;384(23):2177-2186

From the World Mosquito Program Yogyakarta, Center for Tropical Medicine (A.U., C.I., R.A.A., W.T., E.A., M.R.A., E.S., D.S.W., Y.M., I.E., I.N., E.P.), the Department of Health Policy and Management (A.U.), the Department of Biostatistics, Epidemiology, and Public Health (C.I., R.A.A.), and the Department of Child Health (E.A.), Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia; the Department of Biostatistics, School of Public Health, University of California, Berkeley, Berkeley (S.M.D., N.P.J.); the London School of Hygiene and Tropical Medicine, London (N.P.J.); Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam (C.P.S.); and the World Mosquito Program, Institute of Vector-Borne Disease, Monash University, Clayton, VIC, Australia (B.A., B.R.G., L.H., Z.C., E.R., P.A.R., S.L.O., S.M.D., S.K.T., K.L.A., C.P.S.).

Background: mosquitoes infected with the Mel strain of are less susceptible than wild-type to dengue virus infection.

Methods: We conducted a cluster-randomized trial involving releases of Mel-infected mosquitoes for the control of dengue in Yogyakarta, Indonesia. We randomly assigned 12 geographic clusters to receive deployments of Mel-infected (intervention clusters) and 12 clusters to receive no deployments (control clusters). Read More

View Article and Full-Text PDF

Genomic diversity of 39 samples of Pyropia species grown in Japan.

PLoS One 2021 9;16(6):e0252207. Epub 2021 Jun 9.

Faculty of Agriculture, Saga University, Saga, Japan.

Some Pyropia species, such as nori (P. yezoensis), are important marine crops. We conducted a phylogenetic analysis of 39 samples of Pyropia species grown in Japan using organellar genome sequences. Read More

View Article and Full-Text PDF

Reprogramming of ethanol stress response in by the transcription factor Znf1 and the effect on the biosynthesis of glycerol and ethanol.

Appl Environ Microbiol 2021 Jun 9:AEM0058821. Epub 2021 Jun 9.

King Mongkut's University of Technology Thonburi, Division of Biochemical Technology, School of Bioresources and Technology, Bangkok, Thailand.

High ethanol can severely inhibit the growth of yeast cells and fermentation productivity. The ethanologenic yeast activates several well-defined cellular mechanisms of ethanol stress response (ESR); however, the involved regulatory control remains to be characterized. Here, we report a new transcription factor of ethanol stress adaptation called Znf1. Read More

View Article and Full-Text PDF

Silicate minerals as a direct source of limiting nutrients: Siderophore synthesis and uptake promote ferric iron bioavailability from olivine and microbial growth.

Geobiology 2021 Jun 9. Epub 2021 Jun 9.

Department of Earth Sciences, University of Southern California, Los Angeles, CA, USA.

Iron is a micronutrient critical to fundamental biological processes including respiration and photosynthesis, and it can therefore impact primary and heterotrophic productivity. Yet in oxic environments, iron is highly insoluble, rendering it, in principle, unavailable as a nutrient for biological growth. Life has "solved" this problem via the invention of iron chelates, known as siderophores, that keep iron available for microbial productivity. Read More

View Article and Full-Text PDF

Factors influencing the acute pentylenetetrazole-induced seizure paradigm and a literature review.

Ann Clin Transl Neurol 2021 Jun 8. Epub 2021 Jun 8.

F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, 02115, USA.

Objective: To confirm the critical factors affecting seizure susceptibility in acute pentylenetetrazole (PTZ) mouse epilepsy models and evaluate the prior literature for these factors.

Methods: Serial cohorts of wild-type mice administered intraperitoneal (IP)-PTZ were aggregated and analyzed by multivariate logistic regression for the effect of sex, age, background strain, dose, and physiologic stress (i.e. Read More

View Article and Full-Text PDF