2,386 results match your criteria white-rot fungi


Bioremediation of triphenyl phosphate by Pycnoporus sanguineus: Metabolic pathway, proteomic mechanism and biotoxicity assessment.

J Hazard Mater 2021 May 4;417:125983. Epub 2021 May 4.

Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, College of Environmental Science and Engineering, Guilin 541004, Guangxi, China.

So far, no information about the biodegradability of TPhP by white rot fungi has previously been made available, herein, Pycnoporus sanguineus was used as the representative to investigate the potential of white rot fungi in TPhP bioremediation. The results suggested that the biodegradation efficiency of 5 mg/L TPhP by P. sanguineus was 62. Read More

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Phylogeny and diversity of (Polyporales, Basidiomycota), including four new species from South America and Asia.

MycoKeys 2021 26;79:149-172. Epub 2021 Apr 26.

School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China Beijing Forestry University Beijing China.

Four new species of , viz. , , , and , are described from tropical America and Asia. is characterised by dark grey to black pore surface, a monomitic hyphal system, hyaline to yellowish-brown generative hyphae, and ellipsoid basidiospores measuring 3. Read More

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Transcriptional profile of oil palm pathogen, Ganoderma boninense, reveals activation of lignin degradation machinery and possible evasion of host immune response.

BMC Genomics 2021 May 5;22(1):326. Epub 2021 May 5.

Department of Plant Pathology, University of Florida, 1453 Fifield Hall, Gainesville, FL, 32611-0680, USA.

Background: The white-rot fungi in the genus Ganoderma interact with both living and dead angiosperm tree hosts. Two Ganoderma species, a North American taxon, G. zonatum and an Asian taxon, G. Read More

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An overview of from the Neotropics, including four new species.

Mycologia 2021 May 4:1-17. Epub 2021 May 4.

Departamento de Botânica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Prédio 43.433, Campus do Vale, Agronomia, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil.

is a monophyletic genus of polypores that causes white rot of various woody plants. The genus has a worldwide distribution and is ecologically and economically important. Several taxa have been described or reported from the Neotropics, and , described originally from Brazil, is the type species for the genus. Read More

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A Versatile Peroxidase from the Fungus Confers Abiotic Stress Tolerance in Transgenic Tobacco Plants.

Plants (Basel) 2021 Apr 23;10(5). Epub 2021 Apr 23.

División de Estudios de Posgrado e Investigación, Tecnológico Nacional de México/I.T. Roque, Km. 8 Carretera Celaya-Juventino Rosas, Roque, Celaya 38110, Guanajuato, Mexico.

White-rot fungi are efficient lignin degraders due to the secretion of lignin peroxidase, manganese peroxidase, laccase, and versatile peroxidase (VP) on decayed wood. The VP is a high-redox-potential enzyme and could be used to detoxify reactive oxygen species (ROS), which accumulate in plants during biotic and abiotic stresses. We cloned the gene and expressed it via the transformation procedure in transgenic tobacco plants to assay their tolerance to different abiotic stress conditions. Read More

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Enzyme polymer engineered structure strategy to enhance cross-linked enzyme aggregate stability: a step forward in laccase exploitation for cannabidiol removal from wastewater.

Environ Sci Pollut Res Int 2021 Apr 12. Epub 2021 Apr 12.

Université de Sherbrooke Water Research Group, Department of Civil and Building Engineering, Université de Sherbrooke, 2500 boul. de l'Université, Sherbrooke, Québec, J1K 2R1, Canada.

Despite all its advantages and potential, cross-linking enzyme aggregate (CLEA) technology is still not applied at an industrial scale for enzyme insolubilization for bioremediation purposes. In this study, the enzyme polymer engineered structure (EPES) method was used to enhance CLEA stability and reuse. A crude laccase from Trametes hirsuta was successfully insolubilized to form EPES-CLEAs. Read More

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Depolymerization and conversion of lignin to value-added bioproducts by microbial and enzymatic catalysis.

Biotechnol Biofuels 2021 Apr 3;14(1):84. Epub 2021 Apr 3.

National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.

Lignin, the most abundant renewable aromatic compound in nature, is an excellent feedstock for value-added bioproducts manufacturing; while the intrinsic heterogeneity and recalcitrance of which hindered the efficient lignin biorefinery and utilization. Compared with chemical processing, bioprocessing with microbial and enzymatic catalysis is a clean and efficient method for lignin depolymerization and conversion. Generally, lignin bioprocessing involves lignin decomposition to lignin-based aromatics via extracellular microbial enzymes and further converted to value-added bioproducts through microbial metabolism. Read More

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Laccases, Genes Expression and Regulation of Activity.

Biomolecules 2021 Mar 22;11(3). Epub 2021 Mar 22.

Professor Emeritus, School of Food Science, University of Idaho and Washington State University, Moscow, ID 83843, USA.

A white rot fungus has been identified as an important source of laccase, unfortunately regulation of this enzyme genes expression is poorly understood. Using 1D and 2D PAGE and LC-MS/MS, laccase isoenzymes were investigated in the liquid filtrate of culture. The level of expression of laccase genes was measured using qPCR. Read More

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Enhanced Lignocellulolytic Enzyme Activities on Hardwood and Softwood during Interspecific Interactions of White- and Brown-Rot Fungi.

J Fungi (Basel) 2021 Mar 31;7(4). Epub 2021 Mar 31.

Department of Microbiology, Faculty of Agriculture and Forestry, University of Helsinki, FI-00014 Helsinki, Finland.

Wood decomposition is a sophisticated process where various biocatalysts act simultaneously and synergistically on biopolymers to efficiently break down plant cell walls. In nature, this process depends on the activities of the wood-inhabiting fungal communities that co-exist and interact during wood decay. Wood-decaying fungal species have traditionally been classified as white-rot and brown-rot fungi, which differ in their decay mechanism and enzyme repertoire. Read More

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Enzyme Activities of Five White-Rot Fungi in the Presence of Nanocellulose.

J Fungi (Basel) 2021 Mar 18;7(3). Epub 2021 Mar 18.

Laboratory for Cellulose & Wood Materials, Empa, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland.

White-rot fungi can degrade all lignocellulose components due to their potent lignin and cellulose-degrading enzymes. In this study, five white-rot fungi, , , , and were tested for endoglucanase, laccase, urease, and glucose-6-phosphate (G6P) production when grown with malt extract and nanocellulose in the form of TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical) oxidized cellulose nanofibrils (CNF) and cellulose nanocrystals (CNC). Results show that temperature plays a key role in controlling the growth of all five fungi when cultured with malt extract alone. Read More

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Discovery of two novel laccase-like multicopper oxidases from Pleurotus citrinopileatus and their application in phenolic oligomer synthesis.

Biotechnol Biofuels 2021 Apr 1;14(1):83. Epub 2021 Apr 1.

Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens, Greece.

Background: Laccases and laccase-like multicopper oxidases (LMCOs) oxidize a vast array of phenolic compounds and amines, releasing water as a byproduct. Their low substrate specificity is responsible for their tremendous biotechnological interest, since they have been used for numerous applications. However, the laccases characterized so far correspond to only a small fraction of the laccase genes identified in fungal genomes. Read More

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Biogenic synthesis and cytotoxic effects of silver nanoparticles mediated by white rot fungi.

Heliyon 2021 Mar 16;7(3):e06470. Epub 2021 Mar 16.

BCMaterials-Basque Center for Materials, Applications and Nanostructures, Martina Casiano, UPV/EHU Science Park, Barrio Sarriena s/n, Leioa 48940, Spain.

Silver nanoparticles (AgNPs) were successfully synthesized using silver nitrate via the biological route using the culture filtrate of as well as white rot fungi materials at room temperature. The proposed synthetic technique was applied for the first time for AgNPs preparation via the biological route through a low-cost pathway, which considered as an adequate direction of preparation compared to the commercial methods. This study reports the in vitro cytotoxic effect of biologically synthesized AgNPs in disposing of the human lung cancer cell line (A549) and human breast cancer cell (MCF-7) by using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Read More

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(Agaricomycetes, Hymenochaetaceae) in Brazilian Cerrado: Expanding Its Geographic Distribution and Host List.

Front Microbiol 2021 9;12:647920. Epub 2021 Mar 9.

Basic, Applied and Scientific Dissemination Micology Laboratory (FungiLab), Goiás State University, Anápolis, Brazil.

(Pat.) Reid (Agaricomycetes: Hymenochaetaceae) is a poroid fungus characterized by the expressive production of chlamydospores, and , especially during its anamorphic stage. The species plays important ecological roles, standing out as a phytopathogen, affecting several species of ornamental and wild trees, mainly in tropical and subtropical regions. Read More

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Trametes versicolor in lignocellulose-based bioeconomy: State of the art, challenges and opportunities.

Bioresour Technol 2021 Jun 16;330:124997. Epub 2021 Mar 16.

Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 18, HR-31000 Osijek, Croatia.

Although Trametes versicolor is one of the most investigated white-rot fungi, the industrial application of this fungus and its metabolites is still far from reaching its full potential. This review aims to highlight the opportunities and challenges for the industrial use of T. versicolor according to the principles of circular bioeconomy. Read More

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Comparative study of single cultures and a consortium of white rot fungi for polychlorinated biphenyls treatment.

J Appl Microbiol 2021 Mar 16. Epub 2021 Mar 16.

Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones,, CONICET, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Misiones, CP3300, Argentina.

Aims: To evaluate the mycoremediation of polychlorinated biphenyls (PCBs) by either single cultures or binary consortia of Pleurotus pulmonarius LBM 105 and Trametes sanguinea LBM 023.

Methods And Results: PCBs tolerance, removal capacity, toxicity reduction and ligninolytic enzyme expression were assessed when growing single culture and binary consortium of fungus in 217 mg l of a technical mixture of Aroclor 1242, 1254 and 1260 in transformer oil. A decrease in tolerance and variation in ligninolytic enzyme secretion were observed in PCB-amended solid media. Read More

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Investigations on inhibitory effects of nickel and cobalt salts on the decolorization of textile dyes by the white rot fungus Phanerochaete velutina.

Ecotoxicol Environ Saf 2021 Jun 12;215:112093. Epub 2021 Mar 12.

Department of Microbiology, Faculty of Agriculture and Forestry, University of Helsinki, (Biocenter 1, Viikinkaari 9), Finland.

Organic aromatic compounds used for dyeing and coloring in the textile industry are persistent and hazardous pollutants that must be treated before they are discharged into rivers and surface waters. Therefore, we investigated the potential of the white rot fungus Phanerochaete velutina to decolorize commonly used reactive dyes. The fungus decolorized in average 55% of Reactive Orange 16 (RO-16) after 14 days at a maximum rate of 0. Read More

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A comprehensive insight into the application of white rot fungi and their lignocellulolytic enzymes in the removal of organic pollutants.

Sci Total Environ 2021 Mar 1;778:146132. Epub 2021 Mar 1.

Harvard Medical School, Harvard University, Boston, MA 02115, USA. Electronic address:

Environmental problems resultant from organic pollutants are a major current challenge for modern societies. White rot fungi (WRF) are well known for their extensive organic compound degradation abilities. The unique oxidative and extracellular ligninolytic systems of WRF that exhibit low substrate specificity, enable them to display a considerable ability to transform or degrade different environmental contaminants. Read More

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Laccase-mediated delignification and detoxification of lignocellulosic biomass: removing obstacles in energy generation.

Environ Sci Pollut Res Int 2021 Mar 12. Epub 2021 Mar 12.

CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248005, India.

The rising global population and worldwide industrialization have led to unprecedented energy demand that is causing fast depletion of fossil reserves. This has led to search for alternative energy sources that are renewable and environment friendly. Use of lignocellulosic biomass for energy generation is considered a promising approach as it does not compete with food supply. Read More

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Proteomics Reveals Octyl Gallate as an Environmentally Friendly Wood Preservative Leading to Reactive Oxygen Species-Driven Metabolic Inflexibility and Growth Inhibition in White-Rot Fungi ( and ).

J Fungi (Basel) 2021 Feb 17;7(2). Epub 2021 Feb 17.

Department of Forestry, National Chung Hsing University, Taichung 40227, Taiwan.

The most commonly applied wood preservatives are based on creosote, pentachlorophenol, and waterborne chromate copper arsenate, which negatively affect the environment. Thus, environmentally friendly wood preservatives are required. This study investigated the antifungal activity and mechanism of several long-chain alkyl gallates (3,4,5-trihydroxybenzoates) against white-rot fungi, and . Read More

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February 2021

Visualization of Three Sclerotiniaceae Species Pathogenic on Onion Reveals Distinct Biology and Infection Strategies.

Int J Mol Sci 2021 Feb 13;22(4). Epub 2021 Feb 13.

Laboratory of Phytopathology, Wageningen University, 6708 PB Wageningen, The Netherlands.

, , and are three fungal species of the family Sclerotiniaceae that are pathogenic on onion. Despite their close relatedness, these fungi cause very distinct diseases, respectively called leaf blight, neck rot, and white rot, which pose serious threats to onion cultivation. The infection biology of neck rot and white rot in particular is poorly understood. Read More

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February 2021

Assessment of anammox, microalgae and white-rot fungi-based processes for the treatment of textile wastewater.

PLoS One 2021 2;16(3):e0247452. Epub 2021 Mar 2.

Politecnico di Milano, DICA, Milan, Italy.

The treatability of seven wastewater samples generated by a textile digital printing industry was evaluated by employing 1) anammox-based processes for nitrogen removal 2) microalgae (Chlorella vulgaris) for nutrient uptake and biomass production 3) white-rot fungi (Pleurotus ostreatus and Phanerochaete chrysosporium) for decolorization and laccase activity. The biodegradative potential of each type of organism was determined in batch tests and correlated with the main characteristics of the textile wastewaters through statistical analyses. The maximum specific anammox activity ranged between 0. Read More

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Global Phylogeny and Taxonomy of the Wood-Decaying Fungal Genus (Polyporales, Basidiomycota).

Front Microbiol 2021 10;12:622460. Epub 2021 Feb 10.

Beijing Museum of Natural History, Beijing, China.

An in-depth study of the phylogeny and taxonomy of the corticioid genus (Phanerochaetaceae) was conducted. Phylogenetic analyses of the ITS1-5.8S-ITS2 and nrLSU sequences demonstrated that is a strongly supported clade which is distinct from its sister clades of , , and . Read More

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February 2021

Mechanisms of Manganese(II) Oxidation by Filamentous Ascomycete Fungi Vary With Species and Time as a Function of Secretome Composition.

Front Microbiol 2021 10;12:610497. Epub 2021 Feb 10.

Department of Marine Chemistry & Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, MA, United States.

Manganese (Mn) oxides are among the strongest oxidants and sorbents in the environment, and Mn(II) oxidation to Mn(III/IV) (hydr)oxides includes both abiotic and microbially-mediated processes. While white-rot Basidiomycete fungi oxidize Mn(II) using laccases and manganese peroxidases in association with lignocellulose degradation, the mechanisms by which filamentous Ascomycete fungi oxidize Mn(II) and a physiological role for Mn(II) oxidation in these organisms remain poorly understood. Here we use a combination of chemical and in-gel assays and bulk mass spectrometry to demonstrate secretome-based Mn(II) oxidation in three phylogenetically diverse Ascomycetes that is mechanistically distinct from hyphal-associated Mn(II) oxidation on solid substrates. Read More

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February 2021

High-Density Genetic Linkage Map Construction and White Rot Resistance Quantitative Trait Loci Mapping for Genus Based on Restriction Site-Associated DNA Sequencing.

Phytopathology 2021 Apr 26;111(4):659-670. Epub 2021 Feb 26.

College of Horticulture, Shenyang Agricultural University, Shenyang 110866, People's Republic of China.

Grape white rot () is a major fungal disease affecting grape yield and quality. Quantitative trait locus (QTL) analysis is an important method for studying important horticultural traits of grapevine. This study was conducted to construct a high-density map and conduct QTL mapping for grapevine white rot resistance. Read More

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Intracellular pathways for lignin catabolism in white-rot fungi.

Proc Natl Acad Sci U S A 2021 Mar;118(9)

Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401;

Lignin is a biopolymer found in plant cell walls that accounts for 30% of the organic carbon in the biosphere. White-rot fungi (WRF) are considered the most efficient organisms at degrading lignin in nature. While lignin depolymerization by WRF has been extensively studied, the possibility that WRF are able to utilize lignin as a carbon source is still a matter of controversy. Read More

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Evaluation of a Trametes pubescens laccase concentrated extract on allura red AC decolorization without the addition of synthetic mediators.

J Environ Manage 2021 May 18;285:112117. Epub 2021 Feb 18.

Faculty of Science, Universidad Nacional de Colombia, Sede Medellín, 050034, Medellín, Colombia. Electronic address:

Synthetic dye bioremediation is a topic of great importance since these pollutants possess toxic effects, and huge quantities of them are being discharged into water bodies. Ligninolytic enzyme treatment stands out for being a cost-effective methodology, capable of obtaining high decolorization levels. In this work, a laccase enzyme treatment was evaluated to effectively perform a cycle of dye bioremediation. Read More

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Pesticide treatment in biobed systems at microcosms level under critical moisture and temperature range using an Orthic Solonchaks soil from southeastern Mexico amended with corn husk as support.

Sci Total Environ 2021 Jun 2;772:145038. Epub 2021 Feb 2.

Facultad de Ingeniería, Universidad Autónoma de Yucatán, Av. Industrias no Contaminantes por Periférico Norte, Apdo. Postal 150, Cordemex, CP 97310 Mérida, Yucatán, Mexico. Electronic address:

Agriculture effluents from cleaning and handling equipment used in pesticide applications can contaminate superficial and groundwater sources when not correctly disposed of. Biobeds using soil enriched with amendments represent a viable technology to control and minimize pesticide pollution of soil and water in farmlands. They are usually installed outdoors without protection, making them vulnerable to rain flooding, lack of moisture, drought, and intense heat or cold. Read More

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Alternative Splicing of Heat Shock Transcription Factor 2 Regulates the Expression of Laccase Gene Family in Response to Copper in .

Appl Environ Microbiol 2021 Feb 12. Epub 2021 Feb 12.

Laboratory of Bioconversion, Life Science and Technology College, Kunming University of Science and Technology, Kunming, 650500, PR China

White-rot fungi, especially strains, are the primary source of industrial laccases in bioenergy and bioremediation. strains express members of the laccase gene family with different physicochemical properties and expression patterns. However, the literature on the expression pattern of the laccase gene family in S0301 and the response mechanism to Cu, a key laccase inducer, in white-rot fungal strains is scarce. Read More

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February 2021

Characterizing Fungal Decay of Beech Wood: Potential for Biotechnological Applications.

Microorganisms 2021 Jan 26;9(2). Epub 2021 Jan 26.

Laboratory for Cellulose & Wood Materials, Empa-Swiss Federal Laboratories for Materials Science and Technology, CH-9014 St. Gallen, Switzerland.

The biotechnological potential of nine decay fungi collected from stored beech logs at a pulp and paper factory yard in Northern Iran was investigated. Beech blocks exposed to the fungi in a laboratory decay test were used to study changes in cell wall chemistry using both wet chemistry and spectroscopic methods. , , and caused greater lignin breakdown compared to other white-rot fungi, which led to a 28% reduction in refining energy. Read More

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January 2021

Scope and limitations of biocatalytic carbonyl reduction with white-rot fungi.

Bioorg Chem 2021 Mar 12;108:104651. Epub 2021 Jan 12.

Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring, 17, 35392 Giessen, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology, Ohlebergsweg 12, 35392 Giessen, Germany.

The reductive activity of various basidiomycetous fungi towards carbonyl compounds was screened on an analytical level. Some strains displayed high reductive activities toward aromatic carbonyls and aliphatic ketones. Utilizing growing whole-cell cultures of Dichomitus albidofuscus, the reactions were up-scaled to a preparative level in an aqueous system. Read More

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