237,834 results match your criteria western blotting


The influence of rhein on the absorption of rehmaionoside D: In vivo, in situ, in vitro, and in silico studies.

J Ethnopharmacol 2021 Sep 15:114650. Epub 2021 Sep 15.

Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, 712046, China; Co-construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, Xianyang, 712046, China. Electronic address:

Ethnopharmacological Relevance: In traditional Chinese Medicine, Rehmannia glutinosa (Gaertn.) DC., as the principle herb of ShengDiHuang Decotion, has the effect of cooling blood and hemostasis, and tonifying the yin and kidney. Read More

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September 2021

Early-onset pulmonary and cutaneous vasculitis driven by constitutively active SRC-family kinase HCK.

J Allergy Clin Immunol 2021 Sep 15. Epub 2021 Sep 15.

Department of Pediatrics, 2nd Faculty of Medicine, Charles University, and University Hospital Motol, Prague, Czech Republic.

Background: Inborn errors of immunity (IEI) are genetic disorders characterized by various degrees of immune dysregulation that can manifest as immune deficiency, autoimmunity or autoinflammation. The routine use of next-generation sequencing in the clinic has facilitated the identification of an ever-increasing number of IEI, revealing the roles of immunologically important genes in human pathologies. However, despite this progress, treatment is still extremely challenging. Read More

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September 2021

Neuritin attenuates oxygen-glucose deprivation/reperfusion (OGD/R)-induced neuronal injury by promoting autophagic flux.

Exp Cell Res 2021 Sep 15:112832. Epub 2021 Sep 15.

Department of Neurosurgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, 832000, China. Electronic address:

The autophagy/apoptosis interaction has always been a focus of study in pathogenicity models. Neuritin is a neurotrophic factor that is highly expressed primarily in the central nervous system. Our previous study revealed that it protects against apoptosis in cortical neurons subjected to oxygen-glucose deprivation (OGD)/reoxygenation (OGD/R), and later animal experiments revealed that it can increase the expression of the autophagy-related protein LC3. Read More

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September 2021

LHX9, a p53-binding protein, inhibits the progression of glioma by suppressing glycolysis.

Aging (Albany NY) 2021 Sep 17;13(undefined). Epub 2021 Sep 17.

Department of Neurosurgery, XiangYa Hospital of Central South University, Changsha 410078, P.R. China.

Purpose: LHX9 methylation has been reported in many tumors, but its functions and related mechanisms in glioma are still unknown and need to be verified.

Methods: The protein level of LHX9 in glioma tissues was examined using western blotting and immunohistochemistry, and the functions of LHX9 in glioma cell lines were investigated using MTT and colony formation assays. In addition, the interaction between LHX9 and P53 was analyzed by immunoprecipitation, and the roles of LHX9 in cancer metabolism were explored by measuring metabolites. Read More

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September 2021

Selenomethionine Alleviates Intestinal Ischemia-Reperfusion Injury in Mice Through the Bax-Caspase Pathway.

Biol Trace Elem Res 2021 Sep 18. Epub 2021 Sep 18.

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Tech Industrial Development Zone, Daqing, Heilongjiang, 163319, People's Republic of China.

Selenomethionine (SeMet) is known to alleviate ischemia-reperfusion (I/R) injury. However, its details of action have not been thoroughly elucidated in mice with intestinal I/R injury. In this study, intestinal I/R injury mice models were established, and ELISAs were performed to determine the levels of redox factors, including glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA), in mice intestinal tissues. Read More

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September 2021

Radix Tetrastigma Extracts Enhance the Chemosensitivity in Triple-Negative Breast Cancer Via Inhibiting PI3K/Akt/mTOR-Mediated Autophagy.

Clin Breast Cancer 2021 Aug 4. Epub 2021 Aug 4.

Department of Breast Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310006, China. Electronic address:

Objective: Drug resistance in tumors is one of the major factors that leads to chemotherapy failure. This study aims to investigate the effect of Radix Tetrastigma extracts (RTEs) on Taxol-induced autophagy and the chemosensitivity against drug resistance in triple-negative breast cancer (TNBC).

Methods: Taxol-resistant MDA-MB-468 (MDA-MB-468/Taxol) cells were induced and treated with RTEs and/or Taxol. Read More

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LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis.

World J Surg Oncol 2021 Sep 17;19(1):281. Epub 2021 Sep 17.

Department of General Surgery, the First Hospital of Hebei Medical University, No.89 Donggang Road, Shijiazhuang, Hebei, China.

Background: Long noncoding RNAs (lncRNAs) are related to colorectal cancer (CRC) development. However, the role and mechanism of lncRNA LINC01224 in CRC development are largely unknown.

Methods: LINC01224, Yin Yang 1 (YY1), microRNA (miR)-485-5p, and myosins of class VI (MYO6) levels were examined using quantitative reverse transcription polymerase chain reaction and western blotting. Read More

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September 2021

Synthetic Neutralizing Peptides Inhibit the Host Cell Binding of Spike Protein and Block Infection of SARS-CoV-2.

J Med Chem 2021 Sep 17. Epub 2021 Sep 17.

Department of Biomedical Engineering, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.

Antiviral treatments of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been extensively pursued to conquer the pandemic. To inhibit the viral entry to the host cell, we designed and obtained three peptide sequences via quartz crystal microbalance measurement screening, which showed high affinity at nanomole to the S1 subunit of the spike protein and wild-type SARS-CoV-2 pseudovirus. Circular dichroism spectroscopy measurements revealed significant conformation changes of the S1 protein upon encounter with the three peptides. Read More

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September 2021

Mechanism of curcumin against myocardial ischaemia-reperfusion injury based on the P13K/Akt/mTOR signalling pathway.

Eur Rev Med Pharmacol Sci 2021 Sep;25(17):5490-5499

Department of Pharmacy, Yantaishan Hospital, Yantai, China.

Objective: To investigate the pharmacodynamic mechanism of curcumin against myocardial ischaemia-reperfusion injury by regulating the phosphatidylinositol-3 kinase (PI3K)/protein kinase B (AKT)/rapamycin target protein (mTOR) signalling pathway.

Materials And Methods: The left anterior descending coronary artery was ligated for 30 min and reperfused for 3 h to establish an ischaemia-reperfusion injury model. The electrocardiogram (ECG) detection of rats was performed, and the degree of myocardial infarction was determined by 2,3,5-triphenyltetrazolium chloride staining. Read More

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September 2021

The anti-tumour effect of induced pluripotent stem cells against submandibular gland carcinoma in rats is achieved via modulation of the apoptotic response and the expression of Sirt-1, TGF-β, and MALAT-1 in cancer cells.

Mol Cell Biochem 2021 Sep 17. Epub 2021 Sep 17.

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.

The era of induced pluripotent stem cells (iPSCs) was used as novel biotechnology to replace embryonic stem cells bypassing the ethical concerns and problems of stem cell transplant rejection. The anti-tumour potential of iPSCs against many tumours including salivary cancer was proven in previous studies. The current study aimed to investigate the contribution of the Bax, Sirt-1, TGF-β, and MALAT genes and/or their protein expression to the pathogenesis of submandibular carcinogenesis before and after iPSCs treatment. Read More

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September 2021

TGF‑β inhibitor RepSox suppresses osteosarcoma via the JNK/Smad3 signaling pathway.

Int J Oncol 2021 Nov 17;59(5). Epub 2021 Sep 17.

Department of Orthopedic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, P.R. China.

Osteosarcoma (OS) is the most common malignant bone tumor and the long‑term survival rates remain unsatisfactory. Transforming growth factor‑β (TGF‑β) has been revealed to play a crucial role in OS progression, and RepSox is an effective TGF‑β inhibitor. In the present study, the effect of RepSox on the proliferation of the OS cell lines (HOS and 143B) was detected. Read More

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November 2021

[Salvianolic acid B promotes the proliferation, migration and osteogenic differentiation of human gingival mesenchymal stem cells by activating the PI3K/AKT pathway].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Sep;37(9):815-820

National Engineering Laboratory of Hydrometallurgical Cleaner Production Technology, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. *Corresponding author, E-mail:

Objective To investigate the effect of salvianolic acid B (SalB) on the proliferation, migration and differentiation of human gingival mesenchymal stem cells (hGMSCs). Methods The hGMSCs were isolated and cultured, and the expressions of CD73, CD90, CD34, and CD45 were detected by flow cytometry; logarithmic phase cells were selected and hGMSCs were treated with 0, 5, 10 μmol/L SalB for 24 hours. The proliferation activity of cells in each group was detected by CCK-8 assay, the migration ability of cells was detected by Transwell assay and scratch test, and the osteogenic differentiation ability and the adipogenic differentiation ability were detected by alkaline phosphatase (ALP) staining and oil red O staining, respectively; the mRNA and protein expressions of cell differentiation proteins and genes as well as proteins related to the PI3K/AKT signal pathway were detected by Real-time quantiative PCR and Western blotting. Read More

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September 2021

[Knockdown of lysine-specific demethylase 3A (KDM3A) inhibits the invasion and migration of MDA-MB-231 breast cancer cells and arrests the cell cycle in the G0/G1 phase].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Sep;37(9):808-814

Imaging Center of First Affiliated Hospital, Xinjiang Medical University, Urumqi 830000, China. *Corresponding author, E-mail:

Objective To investigate the effect of lysine-specific demethylase 3A (KDM3A) on the invasion and migration of MDA-MB-231 breast cancer cells. Methods The mRNA and the protein expressions of KDM3A in MDA-MB-231 breast cancer cells and MCF-10A normal breast cells were detected by real-time quantitative PCR and Western blotting, respectively; the KDM3A level of MDA-MB-231 cells was knocked down by lentivirus infection of KDM3A short hairpin RNA (shKDM3A). The change of invasion and migration ability of MDA-MB-231 cells was detected by Transwell assay, and the change in the cell cycle was detected by flow cytometry. Read More

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September 2021

[Multi-target kinase inhibitor dasatinib inhibits cell proliferation, dissociation and migration via up-regulating E-cadherin expression in SK-Hep-1 human hepatocellular cancer cells].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Sep;37(9):801-807

Department of Biochemistry and Molecular Biology, School of Basic Medicine, Shanxi Medical University, Taiyuan 030001, China. *Corresponding authors, E-mail:

Objective To investigate the effects of dasatinib (a multi-target kinase inhibitor) on the proliferation, adhesion and migration ability of SK-Hep-1 human hepatocellular cancer cells and the underlying mechanism. Methods SK-Hep-1, Bel-7402, SNU-423, SNU-387 and Huh-7 hepatocellular cancer cells were treated with dasatinib. Then MTT assay was used to detect the effect of dasatinib on the survival and proliferation of cancer cells, and the sensitive cells were obtained. Read More

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September 2021

[Establishment of chimeric antigen receptor NK92MI cells recognizing HBsAg].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Sep;37(9):788-793

Department of Infection Diseases, First Affiliated Hospital, Bengbu Medical College, Core Partner of National Clinical Research Center for Infection Diseases, Anhui Province Key Laboratory of Immunology in Chronic Diseases, Bengbu 233030, China. *Corresponding author, E-mail:

Objective To construct a lentiviral expression vector of chimeric antigen receptor (CAR) recognizing hepatitis B surface antigen (HBsAg) and make it expressed in NK92MI cells. Methods The CAR was designed and synthesized with the sequence of single chain variable fragment (scFv). Next it was cloned into a lentivirus expression vector, followed by packaging, concentration and titer determination. Read More

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September 2021

[RS102895 inhibits the proliferation, invasion, and migration of PC-3 prostate cancer cells by blocking CCL2/CCR2 pathway].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Sep;37(9):781-787

Key laboratory of Xinjiang Endemic and Ethnic Diseases, School of Medicine, Shihezi University, Shihezi 832000, China. *Corresponding author, E-mail:

Objective To investigate the effect of RS102895, a specific C-C motif chemokine receptor 2 (CCR2) antagonist, on the biological behavior of prostate cancer (PCa) cells with different degrees of malignancy. Methods Non-androgen-dependent prostate cancer cells PC-3 and androgen-dependent prostate cancer cells 22RV1 were cultured in vitro. A control group, a recombinant C-C motif chemokine ligand 2 (rCCL2) treatment group, and a rCCL2 combined with RS102895 treatment group were established. Read More

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September 2021

[Knockdown of NLRC3 promotes proliferation and invasion of human colon cancer cells].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Sep;37(9):769-774

Department of Microbiology and Pathogenic Biology, Air Force Medical University, Xi'an 710032, China. *Corresponding authors, E-mail:

Objective To investigate the effects of nucleotide binding oligomerization domain-like receptor family caspase recruitment domain containing 3 (NLRC3) on the proliferation, migration and invasion of human colon cancer HCT116 and LoVo cells. Methods NLRC3 was knocked down in HCT116 and LoVo cells by NLRC3-specific siRNA (si-NLRC3). NLRC3 mRNA and protein expression was detected by real-time quantitative PCR and Western blotting. Read More

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September 2021

Notoginsenoside R2 reduces A25-35-induced neuronal apoptosis and inflammation via miR-27a/SOX8/-catenin axis.

Hum Exp Toxicol 2021 Sep 17:9603271211041996. Epub 2021 Sep 17.

Department of Neurology, 118330The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, China.

Alzheimer's disease (AD) has affected numerous elderly individuals worldwide. has been shown to ameliorate AD symptoms, and notoginsenoside R2 is a key saponin identified in this plant. In the current study, we aimed to explore whether notoginsenoside R2 could improve the prognosis of AD. Read More

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September 2021

CTGF promotes cell-to-cell communication in human periodontal ligament stem cells via MAPK and PI3K pathway.

J Periodontol 2021 Sep 17. Epub 2021 Sep 17.

State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Purposes: Cell-cell communication is an essential process to respond to biological stimuli and sustain the microenvironmental homeostasis of human periodontal ligament stem cells (hPDLSCs). Connective tissue growth factor (CTGF), a critical secreted matrix protein, exhibits significant tasks in regulating the cell-cell and cell-matrix interactions. This study aimed to explore the relationship between CTGF and cell communication and the underlying mechanism. Read More

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September 2021

A newly established monoclonal antibody against ERCC1 detects major isoforms of ERCC1 in gastric cancer.

Glob Health Med 2021 Aug;3(4):226-235

Department of Molecular and Genomic Biomedicine, Center for Bioinformatics and Molecular Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

Identifying patients resistant to cisplatin treatment is expected to improve cisplatin-based chemotherapy for various types of cancers. Excision repair cross-complementing group 1 (ERCC1) is involved in several repair processes of cisplatin-induced DNA crosslinks. ERCC1 overexpression is reported as a candidate prognostic factor and considered to cause cisplatin resistance in major solid cancers. Read More

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Isorhamnetin inhibits amplification of influenza A H1N1 virus inflammation mediated by interferon via the RIG-I/JNK pathway.

Ann Transl Med 2021 Aug;9(16):1327

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Background: Interferon (IFN) amplifies the influenza virus-mediated inflammatory response by forming a paracrine signal feedback loop, which is considered an important cause of excessive inflammatory damage. Isorhamnetin has a wide spectrum of beneficial pharmacological properties, including anti-inflammatory and antiviral effects. The regulatory effect and mechanism of isorhamnetin on influenza virus-mediated inflammation have not yet been reported. Read More

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Effect of growth hormone on thin endometrium via intrauterine infusion.

Ann Transl Med 2021 Aug;9(16):1325

Center of Reproductive Medicine, the First Affiliated Yijishan Hospital of Wannan Medical College, Wuhu, China.

Background: A thin endometrium has become a common reason for the repeated implantation failure. Growth hormone (GH) can regulate the proliferation and metabolism of endometrial cells. The aim of this study was to explore the effect of GH on thin endometrium. Read More

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Tribbles pseudokinase 3 () contributes to the progression of hepatocellular carcinoma by activating the mitogen-activated protein kinase pathway.

Ann Transl Med 2021 Aug;9(15):1253

Department of Pharmacy, Zhuhai People's Hospital, Zhuhai Hospital Affiliated with Jinan University, Zhuhai, China.

Background: Tribble pseudokinase 3 () plays a key role in regulating the malignancy of many tumors. This study examined its function in cancer cells and explored the potential mechanisms of action.

Methods: The expression of was examined in hepatocellular carcinomas (HCCs) using The Cancer Genome Atlas (TCGA) database. Read More

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hsa_circ_0000006 induces tumorigenesis through miR-361-3p targeting immunoglobulin-like domains protein 1 (LRIG1) in osteosarcoma.

Ann Transl Med 2021 Aug;9(15):1242

Department of Orthopaedics, General Hospital of Chinese People's Liberation Army, Beijing, China.

Background: Osteosarcoma (OS) is considered to be the most highly prevalent bone tumor. In the progression of different human cancers, the role of circular RNAs (circRNAs) has been extensively studied. Microarray analysis has indicated that hsa_circ_0000006 expression was lower in OS, but the mechanism of hsa_circ_0000006 in regulating the progression of OS remains elusive. Read More

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Differential Abundance of Brain Mitochondrial Proteins in Yak and Cattle: A Proteomics-Based Study.

Front Vet Sci 2021 31;8:663031. Epub 2021 Aug 31.

Animal Science Department, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, China.

The plateau adaptability and stress resistance of yaks are widely known based on their capacity to survive under severe habitat conditions. However, a few studies on brain mitochondria have characterized these adaptations at the protein level. We identified and quantified the brain mitochondrial proteins using isobaric tags for relative and absolute quantification (iTRAQ) and Proteomics. Read More

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Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.

Transl Androl Urol 2021 Aug;10(8):3402-3414

Department of Urology, The Fifth Medical Centre of Chinese PLA General Hospital, Beijing, China.

Background: Bladder cancer (BC), a common cancer of the urinary system, has a low mortality but an extremely high recurrence rate. Patients who have undergone initial surgical treatment often undergo frequent prognostic examinations with a substantial burden of discomfort and costs. Urine samples can reflect early disease processes in the urinary system and may be an excellent source of biomarkers. Read More

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Proteomic analysis exploring the mechanism of bladder fibrosis induced by ketamine using a rat model.

Transl Androl Urol 2021 Aug;10(8):3300-3311

Department of Urology, Xiangya Hospital, Central South University, Changsha, China.

Background: Long-term abuse of ketamine can cause irreversible bladder fibrosis, but the mechanism of bladder fibrosis is still under investigation. This study aims to explore the mechanism of bladder fibrosis utilizing proteomic analysis in a rat model.

Methods: After building a rat model, hematoxylin and eosin (HE) staining, Masson's trichrome staining, and western blotting (WB) of collagen I were used to assess bladder pathology and fibrosis in a rat model. Read More

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XPA serves as an autophagy and apoptosis inducer by suppressing hepatocellular carcinoma in a PI3K/Akt/mTOR dependent manner.

J Gastrointest Oncol 2021 Aug;12(4):1797-1810

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: To explore the potential biological function of XPA (Xeroderma pigmentosum group A) in hepatic neoplasms and the underlying molecular mechanisms.

Methods: Liver cells were used as experimental models to establish HCC (hepatocellular carcinoma) in vitro. Protein extractions were subjected to Western blotting to detect the proteins expression. Read More

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Endocannabinoid Levels in Ulcerative Colitis Patients Correlate With Clinical Parameters and Are Affected by Cannabis Consumption.

Front Endocrinol (Lausanne) 2021 31;12:685289. Epub 2021 Aug 31.

Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

Background: Inflammatory bowel diseases (IBDs) are chronic, idiopathic, inflammatory, gastrointestinal disorders. The endocannabinoid system may have a role in the pathogenesis of IBD. We aimed to assess whether cannabis treatment influences endocannabinoids (eCBs) level and clinical symptoms of IBD patients. Read More

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Chemerin promotes microangiopathy in diabetic retinopathy via activation of ChemR23 in rat primary microvascular endothelial cells.

Mol Vis 2021 5;27:575-587. Epub 2021 Sep 5.

Tianjin Eye Hospital, Tianjin Key Lab of Ophthalmology and Visual Science, Nankai University Affiliated Eye Hospital, Clinical College of Ophthalmology Tianjin Medical University, Tianjin Eye Institute, Tianjin, China.

Purpose: The correlation between chemerin and diabetic retinopathy (DR) has been demonstrated previously. We aimed to investigate the potential inflammatory and angiogenic roles of chemerin in DR using rat primary retinal microvascular endothelial cells (RRMECs).

Methods: RRMECs were incubated in low- and high-glucose media, and stable chemerin receptor (ChemR23) knockdown in RRMECs was established by lentiviral infection. Read More

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September 2021