23 results match your criteria wells digitized

  • Page 1 of 1

High-accuracy quantitative principle of a new compact digital PCR equipment: Lab On An Array.

Genomics Inform 2021 Sep 30;19(3):e34. Epub 2021 Sep 30.

Department of Bioconvergence Engineering, Dankook University, Yongin 16890, Korea.

Digital PCR (dPCR) is the third-generation PCR that enables real-time absolute quantification without reference materials. Recently, global diagnosis companies have developed new dPCR equipment. In line with the development, the Lab On An Array (LOAA) dPCR analyzer (Optolane) was launched last year. Read More

View Article and Full-Text PDF
September 2021

Digital endpoints for self-administered home-based functional assessment in pediatric Friedreich's ataxia.

Ann Clin Transl Neurol 2021 Sep 6;8(9):1845-1856. Epub 2021 Aug 6.

Translational Medicine, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA.

Background: Friedreich's ataxia is an inherited, progressive, neurodegenerative disease that typically begins in childhood. Disease severity is commonly assessed with rating scales, such as the modified Friedreich's Ataxia Rating Scale, which are usually administered in the clinic by a neurology specialist.

Objective: This study evaluated the utility of home-based, self-administered digital endpoints in children with Friedreich's ataxia and unaffected controls and their relationship to standard clinical rating scales. Read More

View Article and Full-Text PDF
September 2021

10-Plex Digital Polymerase Chain Reaction with Four-Color Melting Curve Analysis for Simultaneous and Genotyping.

Anal Chem 2020 09 19;92(17):11705-11713. Epub 2020 Aug 19.

Biosystems Research Department, Research & Development Group, Hitachi, Ltd.,1-280, Higashi-koigakubo, Kokubunji-shi, Tokyo 185-8601, Japan.

Digital PCR (dPCR) is a promising method for performing liquid biopsies that quantifies nucleic acids more sensitively than real-time PCR. However, dPCR shows large fluctuations in the fluorescence intensity of droplets or wells due to insufficient PCR amplification in the small partitions, limiting the multiplexing capability of using the fluorescence intensity. In this study, we propose a measurement method that combines dPCR with melting curve analysis for highly multiplexed genotyping. Read More

View Article and Full-Text PDF
September 2020

Fast and robust sample self-digitization for digital PCR.

Anal Chim Acta 2020 Apr 7;1107:127-134. Epub 2020 Feb 7.

Key Laboratory of Optoelectronic Technology and Systems, Ministry of Education, Defense Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University, Chongqing, 400044, China. Electronic address:

We present a facile sample partitioning method to enable rapid and low-cost digital PCR (dPCR) assays. By subdividing a high percentage of the sample volume into a large number of equal volume compartments with a self-digitization (SD) chip, this method can achieve a low-waste and high-order sample discretization in a matter of minutes. The SD chip contains a set of parallel microfluidic channels used for sample delivery, and each channel is connected with two rows of cylindrical wells to hold the discretized sample. Read More

View Article and Full-Text PDF

Blockchain Implementation in Health Care: Protocol for a Systematic Review.

JMIR Res Protoc 2019 Feb 8;8(2):e10994. Epub 2019 Feb 8.

Healthcare Translation Research Group, Department of Paediatrics, University of Oxford, Oxford, United Kingdom.

Background: A blockchain is a digitized, decentralized, distributed public ledger that acts as a shared and synchronized database that records cryptocurrency transactions. Despite the shift toward digital platforms enabled by electronic medical records, demonstrating a will to reform the health care sector, health systems face issues including security, interoperability, data fragmentation, timely access to patient data, and silos. The application of health care blockchains could enable data interoperability, enhancement of precision medicine, and reduction in prescription frauds through implementing novel methods in access and patient consent. Read More

View Article and Full-Text PDF
February 2019

Light scattering measured with spatial frequency domain imaging can predict stromal versus epithelial proportions in surgically resected breast tissue.

J Biomed Opt 2018 09;24(7):1-11

Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States.

This study aims to determine if light scatter parameters measured with spatial frequency domain imaging (SFDI) can accurately predict stromal, epithelial, and adipose fractions in freshly resected, unstained human breast specimens. An explicit model was developed to predict stromal, epithelial, and adipose fractions as a function of light scattering parameters, which was validated against a quantitative analysis of digitized histology slides for N  =  31 specimens using leave-one-out cross-fold validation. Specimen mean stromal, epithelial, and adipose volume fractions predicted from light scattering parameters strongly correlated with those calculated from digitized histology slides (r  =  0. Read More

View Article and Full-Text PDF
September 2018

Oil and gas development footprint in the Piceance Basin, western Colorado.

Sci Total Environ 2018 Mar 8;616-617:355-362. Epub 2017 Nov 8.

Northern Rocky Mountain Science Center, U.S. Geological Survey, 2317 University Way St. 2, Bozeman, MT 59715, United States. Electronic address:

Understanding long-term implications of energy development on ecosystem function requires establishing regional datasets to quantify past development and determine relationships to predict future development. The Piceance Basin in western Colorado has a history of energy production and development is expected to continue into the foreseeable future due to abundant natural gas resources. To facilitate analyses of regional energy development we digitized all well pads in the Colorado portion of the basin, determined the previous land cover of areas converted to well pads over three time periods (2002-2006, 2007-2011, and 2012-2016), and explored the relationship between number of wells per pad and pad area to model future development. Read More

View Article and Full-Text PDF

Geospatial Assessment of Cholera in a Rapidly Urbanizing Environment.

J Environ Public Health 2017 11;2017:6847376. Epub 2017 Apr 11.

Institute of Ecology and Environmental Studies, OAU, Ile-Ife, Osun State, Nigeria.

This study mapped out and investigated the spatial relationship between cholera incidences and environmental risk factors in the study area. The study area was stratified into eight zones. Water samples from each zone were collected and analyzed to determine the colony forming units. Read More

View Article and Full-Text PDF
November 2017

Using domestic well records to determine fractured bedrock watersheds and recharge rates.

Ground Water 2014 Sep-Oct;52(5):782-8. Epub 2013 Sep 19.

Department of Natural Resources and the Environment, University of Connecticut, Storrs, CT 06269.

This study presents an approach for delineating groundwater basins and estimating rates of recharge to fractured crystalline bedrock. It entailed the use of completion report data (boring logs) from 2500 domestic wells in bedrock from the Coventry Quadrangle, which is located in northeastern Connecticut and characterized by metamorphic gneiss and schist. Completion report data were digitized and imported into ArcGIS(®) for data analysis. Read More

View Article and Full-Text PDF

Sensitive chemiluminescent immunoassay of triclopyr by digital image analysis.

Talanta 2012 Aug 10;97:42-7. Epub 2012 May 10.

Departamento de Química Analítica, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos s/n 29071 Málaga, Spain.

An image based detection of chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) for the quantification of triclopyr has been developed. The immunoassay was an indirect competitive immunoassay with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (HRP). Chemiluminescence was produced by the luminol/H(2)O(2)/HRP reaction, detected by a monochrome video CCD camera and digitized with an Imagraph IC-PCI frame grabber using a custom program developed in C(++) (Microsoft Visual C(++) 6. Read More

View Article and Full-Text PDF

Groundwater contamination by nitrates in the city of Konya, (Turkey): a GIS perspective.

J Environ Manage 2006 Apr 6;79(1):30-7. Epub 2005 Sep 6.

Department of Environmental Engineering, Selcuk University, 42079 Konya, Turkey.

Groundwater is an essential drinking water source in the city of Konya, Turkey. Approximately 75% of the city's water consumption has been supplied from 198 groundwater wells for the last six years. Nitrate (NO(3)(-)) is one of the important water quality parameters and was measured in the water samples taken from 139 wells in 1998 and from 156 wells in 2001 within the study area of 427. Read More

View Article and Full-Text PDF

A new visualization technique for laparoscopic ultrasonography.

Surgery 2004 Jul;136(1):84-92

Department of Surgery, Massachusetts General Hospital, Boston, 02114, USA.

Background: Using laparoscopic ultrasonography (LUS) is challenging for both novice and experienced ultrasonographers. The major difficulty surgeons experience is understanding the orientation of the ultrasonography image. The purpose of this study was to assess whether providing surgeons with orientation information improves their ability to interpret LUS images. Read More

View Article and Full-Text PDF

Radiopacity of endodontic sealers: development of a new method for direct measurement.

J Endod 2003 Nov;29(11):751-5

Department of Endodontology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Israel.

Radiopacity is an essential attribute of endodontic filling materials. It is important to see clearly the root canal filling to detect its presence, extent, and apparent condensation. National and international standards require a minimal radiopacity equivalent to 3 or 4 mm of aluminum, yet some products made by reputed companies do not meet this requirement. Read More

View Article and Full-Text PDF
November 2003

Cervical spinal motion during intubation: efficacy of stabilization maneuvers in the setting of complete segmental instability.

J Neurosurg 2001 Apr;94(2 Suppl):265-70

Department of Anesthesia and Radiology, The University of Iowa Hospitals and Clinics, Iowa City 52242, USA.

Object: The purpose of this study was to characterize and compare segmental cervical motion during orotracheal intubation in cadavers with and without a complete subaxial injury, as well as to examine the efficacy of commonly used stabilization techniques in limiting that motion.

Methods: Intubation procedures were performed in 10 fresh human cadavers in which cervical spines were intact and following the creation of a complete C4-5 ligamentous injury. Movement of the cervical spine during direct laryngoscopy and intubation was recorded using video fluoroscopy and examined under the following conditions: 1) without stabilization; 2) with manual in-line cervical immobilization; and 3) with Gardner-Wells traction. Read More

View Article and Full-Text PDF

Segmental cervical spine motion during orotracheal intubation of the intact and injured spine with and without external stabilization.

J Neurosurg 2000 Apr;92(2 Suppl):201-6

Division of Neurosurgery, The University of Iowa Hospitals and Clinics, Iowa City 52242, USA.

Object: The purpose of this study was to establish a cadaveric model for evaluating cervical spine motion in both the intact and injured states and to examine the efficacy of commonly used stabilization techniques in limiting that motion.

Methods: Intubation was performed in fresh human cadavers with intact cervical spines, following the creation of a C4-5 posterior ligamentous injury. Movement of the cervical spine during direct laryngoscopy and intubation was recorded using video fluoroscopy and examined under the following conditions: 1) without external stabilization; 2) with manual in-line cervical immobilization; and 3) with Gardner-Wells traction. Read More

View Article and Full-Text PDF

Comparison of four peak spinal loading exposure measurement methods and their association with low-back pain.

Scand J Work Environ Health 1999 Oct;25(5):404-9

Department of Kinesiology, University of Waterloo, Ontario, Canada.

Objectives: This paper examines the performance of 4 different methods of estimating peak spinal loading and their relationship with the reporting of low-back pain.

Methods: The data used for this comparison was a subset of subjects from a case-referent study of low-back-pain reporting in the automotive industry, in which 130 random referents and 105 cases (or job-matched proxies) were studied. The peak load on the lumbar spine was determined using a biomechanical model with model inputs coming from a detailed self-report questionnaire, a task-based check list, a video digitization method, and a posture and load sampling technique. Read More

View Article and Full-Text PDF
October 1999

Changes in geometry of the finger flexor tendons in the carpal tunnel with wrist posture and tendon load: an MRI study on normal wrists.

Authors:
P J Keir R P Wells

Clin Biomech (Bristol, Avon) 1999 Nov;14(9):635-45

Department of Kinesiology, York University, 364 Bethune College, 4700 Keele Street, North York, Toronto, Ontario, Canada.

Objectives: (1) To develop a methodology to determine the trajectories of the digital flexor tendons using MRI. (2) To examine changes in tendon trajectories due to wrist posture, with and without pinch force. (3) To calculate the radius of curvature of the flexor tendons and note implications for contact forces on the median nerve. Read More

View Article and Full-Text PDF
November 1999

Ratiometric measurement of intracellular pH of cultured cells with BCECF in a fluorescence multi-well plate reader.

Authors:
R L Grant D Acosta

In Vitro Cell Dev Biol Anim 1997 Apr;33(4):256-60

Division of Pharmacology & Toxicology, College of Pharmacy, University of Texas at Austin 78712-1074, USA.

A number of methods have been developed to measure intracellular pH (pHi) because of its importance in intracellular events. A major advance in accurate pHi measurement was the development of the ratiometric fluorescent indicator dye, 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF). We have used a fluorescence multi-well plate reader and a ratiometric method for determining pHi in primary cultures of rabbit corneal epithelial (CE) cells with BCECF. Read More

View Article and Full-Text PDF

Cytofluorescent assay to quantify adhesion of equine spermatozoa to oviduct epithelial cells in vitro.

Authors:
P G Thomas B A Ball

Mol Reprod Dev 1996 Jan;43(1):55-61

Department of Clinical Sciences, Cornell University, Ithaca, NY 14853, USA.

To facilitate the study of interactions between equine spermatozoa and homologous oviduct epithelial cells, we developed an assay to count labelled spermatozoa bound to oviduct epithelial cell (OEC) monolayers and used the assay to compare the binding ability of spermatozoa from different stallions. Washed spermatozoa from three stallions were incubated with the fluorochrome Hoechst 33342 (5 micrograms/ml) for 1 min. Spermatozoa were then layered over confluent monolayers of oviduct epithelial cells in 2 cm2 culture wells. Read More

View Article and Full-Text PDF
January 1996

Computer- and robot-assisted resection of thalamic astrocytomas in children.

Neurosurgery 1991 Jul;29(1):27-33

Division of Neurosurgery, Hospital for Sick Children, Toronto, Ontario, Canada.

Six children ranging in age from 2 to 10 years who harbored deep benign astrocytomas were operated upon using a computer- and robot-assisted system. A radical excision was achieved in all cases with no significant morbidity nor any mortality. The system consists of an interactive, three-dimensional display of computed tomographic image contours and digitized cerebral angiograms taken using the Brown-Roberts-Wells stereotactic frame. Read More

View Article and Full-Text PDF

Detection of ventricular tachycardia using scanning correlation analysis.

Pacing Clin Electrophysiol 1990 Dec;13(12 Pt 2):1930-6

Telectronics Pacing Systems, Englewood, Colorado 80112.

Cross correlation is an accurate method for distinguishing normal sinus rhythm (NSR) from ventricular arrhythmias. The computational demands of the method, however, have prohibited development of an implantable device using correlation. In this study, temporal data compression prior to correlation analysis was used to reduce the total number of computations. Read More

View Article and Full-Text PDF
December 1990

Automated surface area measurement of cultured cardiac myocytes.

Cytotechnology 1990 Sep;4(2):155-61

Centers for Disease Control, National Institute for Occupational Safety and Health, Cincinnati, OH 45226.

An automated method for rapidly measuring surface area of individual cardiac myocytes was used as an index of myocyte growth. Hearts from 2- to 4-day-old rats were digested by overnight incubation in cold trypsin solution. Enriched suspensions of myocytes were plated at 2 x 10(5) cells/well in 12-well-culture plates. Read More

View Article and Full-Text PDF
September 1990

New scans from old: digital reformatting of ultrasound images.

Br J Radiol 1989 Sep;62(741):824-9

Bristol General Hospital.

Two-dimensional images obtained using ultrasound have been digitized from videotape recordings and stored within a maximum of 240 digital memory planes to form a three-dimensional data set using a commercially available image processing unit. This data set has been manipulated to produce images in planes perpendicular to the original scan set. The reformatted images represent not only the scans that could have been obtained by rotating the scan head but also demonstrate planes that are not accessible by conventional scanning. Read More

View Article and Full-Text PDF
September 1989
  • Page 1 of 1