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Assocation Between Leber's Hereditary Optic Neuropathy and MT-ND1 3460G>A Mutation-Induced Alterations in Mitochondrial Function, Apoptosis, and Mitophagy.

Invest Ophthalmol Vis Sci 2021 Jul;62(9):38

Attardi Institute of Mitochondrial Biomedicine, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Purpose: To investigate the molecular mechanism underlying the Leber's hereditary optic neuropathy (LHON)-linked MT-ND1 3460G>A mutation.

Methods: Cybrid cell models were generated by fusing mitochondrial DNA-less ρ0 cells with enucleated cells from a patient carrying the m.3460G>A mutation and a control subject. Read More

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Genomic evidence for speciation with gene flow in broadcast spawning marine invertebrates.

Mol Biol Evol 2021 Jul 26. Epub 2021 Jul 26.

Fisheries Laboratory, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 2971-4, Bentenjima, Maisaka, 431-0214, Japan Hamamatsu, Shizuoka.

How early stages of speciation in free-spawning marine invertebrates proceeds is poorly understood. The Western Pacific abalones, Hatiois discus, H. madaka, and H. Read More

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Efficient degradation of Bisphenol A by dielectric barrier discharge non-thermal plasma: Performance, degradation pathways and mechanistic consideration.

Chemosphere 2021 Jul 21;286(Pt 1):131627. Epub 2021 Jul 21.

School of Environmental Science & Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address:

The discharge of recalcitrant and persistent organic pollutants into the environment and subsequent adverse impacts on the ecosystem has aroused a great concern all over the world. In this study, dielectric barrier discharge (DBD) non-thermal plasma was employed to eliminate bisphenol A (BPA). The influences of several vital experimental parameters, including discharge voltage, initial pH of solution, and rate of water flow on degradation of BPA, were explored in detail. Read More

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A dynamic, convenient and accurate method for assessing the flood risk of people and vehicle.

Sci Total Environ 2021 Jul 18;797:149036. Epub 2021 Jul 18.

Beijing Capital Co Ltd, Beijing 100028, China.

With the increase of extreme rainstorm caused by climate change, and the development of urbanization and the improvement of people's living standard, there is an urgent need to draw a dynamic, convenient and accurate flood risk map for different disaster bearing bodies, so as to protect people's lives and properties, as well as improve people's risk awareness and facilitate people's lives. This study mainly researched a method of drawing a dynamic, convenient and accurate flood risk map for people and vehicle. In this study, the surface runoff is simulated by GPU (Graphics Processing Unit) Accelerated Surface Water Flow and Transport model (GAST model), meanwhile, the flood risk of people and vehicle is graded base on the incipient velocity formulas, the most unfavorable principle and grading method, finally, the method is applied in two application cases. Read More

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Mitotropic Triphenylphosphonium Doxorubicin-Loaded Core-Shell Nanoparticles for Cellular and Mitochondrial Sequential Targeting of Breast Cancer.

Int J Pharm 2021 Jul 23:120936. Epub 2021 Jul 23.

Nanomedicine Labs, Center for Materials Science (CMS), Zewail City of Science and Technology, 6 of October City, 12578, Giza, Egypt. Electronic address:

Hypotheses Targeted therapy exploits cancerous niches' properties including acidic extracellular environment, hypoxic tumor core, and tumor-specific surface antigens. The present study aims to develop and evaluate a sequential targeted core-shell nanoparticulate (NPs) system for treatment of breast cancer. Sequential (double-stage) targeting was achieved at the cellular-level employing the selective CD44- receptor binding hyaluronic acid (HA), followed by subcellular mitochondrial drug-delivery using a synthesized triphenylphosphonium-conjugated doxorubicin (DOX-TPP). Read More

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Differential involvement of programmed cell death ligands in skin immune responses.

J Invest Dermatol 2021 Jul 23. Epub 2021 Jul 23.

Department of Dermatology, Faculty of Medicine, University of Tsukuba, Japan. Electronic address:

Programmed cell death-1 (PD-1) is an immuno-regulatory receptor that can bind PD-L1 or PD-L2 expressed on stimulated antigen-presenting cells. In this study, isolated antigen-presenting cells (macrophages and dendritic cells) were cultured with interferon (IFN)-γ, interleukin (IL)-4, or IL-17A and expression of PD-L1 and PD-L2 was compared by flow cytometry. Strong upregulation of PD-L1 expression was observed upon IFN-γ stimulation of both antigen-presenting cells, as well as in response to IL-17A stimulation of macrophages compared to unstimulated controls. Read More

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Long non-coding RNA H19 acts as a microRNA-194 sponge to inhibit the apoptosis and promote the proliferation of hypertrophic scar fibroblasts.

Can J Physiol Pharmacol 2021 Jul 26. Epub 2021 Jul 26.

Medical School of Eastern Liaoning University, Department of Cosmetic Dermatology, Dandong, China;

The effects of long non-coding RNAs (lncRNAs) on the proliferation of hypertrophic scars have been described. However, the underlying mechanisms are not well characterized. The present study aimed to investigate the mechanisms of lncRNA H19 in hypertrophic scars. Read More

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Kidney damage from nonsteroidal anti-inflammatory drugs-Myth or truth? Review of selected literature.

Pharmacol Res Perspect 2021 Aug;9(4):e00817

Department of Pharmacokinetics and Monitored Therapy, Pomeranian Medical University, Szczecin, Poland.

Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely available drugs with anti-inflammatory and analgesic properties. Their mechanism of action is associated with the enzymes of the arachidonic acid cycle (cyclooxygenases: COX-1 and COX-2). The cyclooxygenase pathway results in the formation of prostanoids (prostaglandins [PGs], prostacyclins, and thromboxanes). Read More

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Clinical and laboratory findings in patients with leukocyte adhesion deficiency type I: A multicenter study in turkey.

Clin Exp Immunol 2021 Jul 26. Epub 2021 Jul 26.

Division of Pediatric Immunology, Department of Pediatrics, Hacettepe University Medical School, Ankara, Turkey.

Leukocyte adhesion deficiency type I is rare primary immunodeficiency disorder characterized by mutations in the ITGB2 gene encoding CD18. We present clinical and immunological features of 15 patients with leukocyte adhesion deficiency type I (LAD-I). Targeted next-generation sequencing was performed with either a primary immunodeficiency gene panel comprising 266 genes or a small LAD-panel consisting of five genes for genetic analysis. Read More

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Down-regulation of circRNA_0000143 regulates miR-142-3p/GRα axis to facilitate the progression of T-ALL.

Cancer Sci 2021 Jul 26. Epub 2021 Jul 26.

Department of Hematology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, 130021, Anhui, China.

Circular RNAs (circRNAs) regulate gene expression in eukaryotes and participate in the progression of malignancy. Instead, the function and underlying mechanisms of circ_0000143 in T-cell acute lymphoblastic leukemia (T-ALL) remain unclear. In this study, quantitative real-time polymerase chain reaction (qRT-PCR) was executed to probe circ_0000143 expression in bone marrow samples from T-ALL patients. Read More

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Follicular Helper and Follicular Regulatory T Cell Subsets Imbalance is Associated with higher activated B cells and Abnormal Auto-antibody Production in Primary Anti-phospholipid Syndrome Patients.

Clin Exp Immunol 2021 Jul 26. Epub 2021 Jul 26.

Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.

Primary anti-phospholipid antibody syndrome (pAPS) is a multi-organs autoimmune disease, and autoantibodies were involved in its pathogenesis. Follicular helper T cells (Tfh) and follicular regulatory T cells (Tfr) are critical for B cells maturation and antibodies production, but their roles in pAPS remain unknown. We enrolled Thirty-two pAPS patients and twenty-three healthy controls (HCs) and comprehensively analyzed circulating Tfh and Tfr, as well as their subsets, using flow cytometry. Read More

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DNA Electrochemistry: Charge-Transport Pathways through DNA Films on Gold.

J Am Chem Soc 2021 Jul 26. Epub 2021 Jul 26.

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.

Over the past 25 years, collective evidence has demonstrated that the DNA base-pair stack serves as a medium for charge transport chemistry in solution and on DNA-modified gold surfaces. Since this charge transport depends sensitively upon the integrity of the DNA base pair stack, perturbations in base stacking, as may occur with DNA base mismatches, lesions, and protein binding, interrupt DNA charge transport (DNA CT). This sensitivity has led to the development of powerful DNA electrochemical sensors. Read More

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[Differentiation of Hydrogen and Oxygen Isotopes in the Water Source Treatment Wetlands of Stream Networks].

Huan Jing Ke Xue 2021 Aug;42(8):3695-3708

Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

To explore the isotopic distribution and differentiation of water along the hydraulic flow gradients and plant-bed/ditch systems in constructed root-channel wetlands, surface and subsurface water samples were collected from four ecological wetlands, namely Shijiuyang and Guanjinggang in Jiaxing, as well as Changshuitang and Taishangang in Haining. All samples were collected along water flow pathways during the wet and rainy summer season in August 2019, except for those from Taishangang, which were collected within the plant-bed/ditch system during the dry and cold winter season in January 2020. The abundance of deuterium (D) and O was determined in each functional area of the wetlands to assess the influence of wetlands on water differentiation. Read More

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A microfluidic system for monitoring glucagon secretion from human pancreatic islets of Langerhans.

Anal Methods 2021 Jul 26. Epub 2021 Jul 26.

Department of Chemistry and Biochemistry, Florida State University, 95 Chieftain Way, Tallahassee, FL 32306, USA.

Glucagon is a 29-amino acid peptide released from α-cells within pancreatic islets of Langerhans to help raise blood glucose levels. While a plethora of methodologies have been developed for quantitative measurement of insulin released from islets, such methods are not well developed for glucagon despite its importance in blood sugar regulation. In this work, a simple yet robust microfluidic device was developed for holding human pancreatic islets and perfuse them with glucose. Read More

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COVID-19-Related Spontaneous Aortic Arch Thrombus With Delayed Embolization.

Am Surg 2021 Jul 25:31348211034744. Epub 2021 Jul 25.

Division of Vascular Surgery and Endovascular Therapy, Loyola University Health System, Maywood, IL, USA.

Presentation of a 62-year-old man with baseline chronic obstructive pulmonary disease admitted to the hospital with dyspnea and newly diagnosed COVID-19 infection. CT scan of the chest was obtained to rule out pulmonary embolism. This revealed a mural thrombus of the inner curvature of the aortic arch with a floating component. Read More

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Reaction Rate Coefficient of OH Radicals with -Butanol as a Function of Temperature.

ACS Omega 2021 Jul 8;6(28):18123-18134. Epub 2021 Jul 8.

IMT Lille Douai, Univ. Lille, SAGE, Lille F-59000, France.

-Butanol or 1-butan- -ol (D9B) is often used as an OH radical tracer in atmospheric chemistry studies to determine OH exposure, a useful universal metric that describes the extent of OH radical oxidation chemistry. Despite its frequent application, there is only one study that reports the rate coefficient of D9B with OH radicals, (295 K), which limits its usefulness as an OH tracer for studying processes at temperatures lower or higher than room temperature. In this study, two complementary experimental techniques were used to measure the rate coefficient of D9B with OH radicals, (), at temperatures between 240 and 750 K and at pressures within 2-760 Torr. Read More

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Inactivation of Cerebral Cavernous Malformation Genes Results in Accumulation of von Willebrand Factor and Redistribution of Weibel-Palade Bodies in Endothelial Cells.

Front Mol Biosci 2021 9;8:622547. Epub 2021 Jul 9.

Department of Human Genetics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.

Cerebral cavernous malformations are slow-flow thrombi-containing vessels induced by two-step inactivation of the , or gene within endothelial cells. They predispose to intracerebral bleedings and focal neurological deficits. Our understanding of the cellular and molecular mechanisms that trigger endothelial dysfunction in cavernous malformations is still incomplete. Read More

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Usefulness of Stress-Derived E/e' Ratio in Asymptomatic Hypertensive Patients.

Pulse (Basel) 2021 Jun 25;8(3-4):92-98. Epub 2020 Nov 25.

Department of Cardiology, Zagazig University Hospital, Zagazig, Egypt.

Purpose: We sought to investigate the usefulness of stress echocardiography-derived E/e' in predicting subclinical atherosclerosis in asymptomatic hypertensive patients.

Materials And Methods: 71 newly diagnosed untreated hypertensive patients (48 ± 13 years, 65% males) and 30 age- and sex-matched healthy controls were recruited. Resting and exercise echocardiography was performed to assess resting diastolic blood pressure and the diastolic stress parameters. Read More

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Plasma LOX-Products and Monocyte Signaling Is Reduced by Adjunctive Cyclooxygenase-2 Inhibitor in a Phase I Clinical Trial of Tuberculosis Patients.

Front Cell Infect Microbiol 2021 9;11:669623. Epub 2021 Jul 9.

Institute of Clinical Medicine, University of Oslo, Oslo, Norway.

Introduction: Eicosanoids and intracellular signaling pathways are potential targets for host-directed therapy (HDT) in tuberculosis (TB). We have explored the effect of cyclooxygenase 2 inhibitor (COX-2i) treatment on eicosanoid levels and signaling pathways in monocytes.

Methods: Peripheral blood mononuclear cells isolated from TB patients included in a randomized phase I clinical trial of standard TB treatment with (n=21) or without (n=18) adjunctive COX-2i (etoricoxib) were analyzed at baseline, day 14 and day 56. Read More

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Information flow to increase support for tidal energy development in remote islands of a developing country: agent-based simulation of information flow in Flores Timur Regency, Indonesia.

Energy Sustain Soc 2021 21;11(1):26. Epub 2021 Jul 21.

Department of Ocean Technology, Policy, and Environment, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba Japan.

Background: Public awareness is crucial for successful deployment of tidal energy, a renewable energy source that can provide clean electricity to remote islands. However, considering public attitudes on tidal energy are not well known, especially in developing countries, a barrier exists in implementing public engagement strategies. This study aims to contribute by identifying strategies for information provision-the initial step in public engagement-and estimate how these can be engaged to enhance support for tidal energy among the local public in a remote area of a developing country, in this case, Flores Timur Regency, Indonesia, considering their socio-cultural background. Read More

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A Deep Neural Network Based Glottal Flow Model for Predicting Fluid-Structure Interactions during Voice Production.

Appl Sci (Basel) 2020 Jan 19;10(2). Epub 2020 Jan 19.

Department of Mechanical Engineering, University of Maine, Orono, ME 04469, USA.

This paper proposes a machine-learning based reduced-order model that can provide fast and accurate prediction of the glottal flow during voice production. The model is based on the Bernoulli equation with a viscous loss term predicted by a deep neural network (DNN) model. The training data of the DNN model is a Navier-Stokes (N-S) equation-based three-dimensional simulation of glottal flows in various glottal shapes generated by a synthetic shape function, which can be obtained by superimposing the instantaneous modal displacements during vibration on the prephonatory geometry of the glottal shape. Read More

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January 2020

FUNCTIONALIZED ELECTROSPUN POLYMER NANOFIBERS FOR TREATMENT OF WATER CONTAMINATED WITH URANIUM.

Environ Sci (Camb) 2020 Mar 12;6(3):622-634. Epub 2019 Dec 12.

Department of Civil and Environmental Engineering, University of Iowa, 4105 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA 52242.

Uranium (U) contamination of drinking water often affects communities with limited resources, presenting unique technology challenges for U treatment. Here, we develop a suite of chemically functionalized polymer (polyacrylonitrile; PAN) nanofibers for low pressure reactive filtration applications for U removal. Binding agents with either nitrogen-containing or phosphorous-based (e. Read More

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Cord blood group 2 innate lymphoid cells are associated with lung function at 6 weeks of age.

Clin Transl Immunology 2021 21;10(7):e1296. Epub 2021 Jul 21.

Priority Research Centre GrowUpWell® - Hunter Medical Research Institute The University of Newcastle Newcastle NSW Australia.

Objective: Offspring born to mothers with asthma in pregnancy are known to have lower lung function which tracks with age. Human group 2 innate lymphoid cells (ILC2) accumulate in foetal lungs, at 10-fold higher levels compared to adult lungs. However, there are no data on foetal ILC2 numbers and the association with respiratory health outcomes such as lung function in early life. Read More

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Southern introgression increases adaptive immune gene variability in northern range margin populations of Fire-bellied toad.

Ecol Evol 2021 Jul 27;11(14):9776-9790. Epub 2021 Jun 27.

Unit of Evolutionary Biology/Systematic Zoology Institute of Biochemistry and Biology University of Potsdam Potsdam Germany.

Northern range margin populations of the European fire-bellied toad () have rapidly declined during recent decades. Extensive agricultural land use has fragmented the landscape, leading to habitat disruption and loss, as well as eutrophication of ponds. In Northern Germany (Schleswig-Holstein) and Southern Sweden (Skåne), this population decline resulted in decreased gene flow from surrounding populations, low genetic diversity, and a putative reduction in adaptive potential, leaving populations vulnerable to future environmental and climatic changes. Read More

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Variation in heteroploid reproduction and gene flow across a polyploid complex: One size does not fit all.

Ecol Evol 2021 Jul 29;11(14):9676-9688. Epub 2021 Jun 29.

Department of Biology University of Virginia Charlottesville VA USA.

Whole-genome duplication is considered an important speciation mechanism in plants. However, its effect on reproductive isolation between higher cytotypes is not well understood. We used backcrosses between different ploidy levels and surveys of mixed-ploidy contact zones to determine how reproductive barriers differed with cytotype across a polyploid complex. Read More

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The effect of montelukast sodium plus budesonide on the clinical efficacy, inflammation, and pulmonary function in children with cough variant asthma.

Am J Transl Res 2021 15;13(6):6807-6816. Epub 2021 Jun 15.

Department of Pediatrics, Affiliated Nanhua Hospital, University of South China Hengyang 421000, Hunan Province, China.

Objective: The aim of this study is to explore the clinical efficacy of montelukast sodium (MKST) combined with budesonide (BUD) on children with cough variant asthma (CVA) and its influence on inflammation and pulmonary function (PF).

Methods: One hundred and sixty-six children with CVA treated in the Affiliated Nanhua Hospital, University of South China from May 2017 to August 2019 were randomized into a joint group (JG, n=92) for the combination therapy of MKST and BUD, and a control group (CG, n=74) for BUD monotherapy. Their clinical symptoms, total response rates (RR), PF, and inflammatory factor expressions were evaluated before and after treatment. Read More

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Circ_0057452 functions as a ceRNA in hypertrophic scar fibroblast proliferation and VEGF expression by regulating TGF-β2 expression and adsorbing miR-145-5p.

Am J Transl Res 2021 15;13(6):6200-6210. Epub 2021 Jun 15.

Department of Burn and Plastic Surgery, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health and Disorders Hangzhou, Zhejiang Province, China.

Objective: To explore the mechanism by which circ_0057452/miR-145-5p/TGF-β2 axis regulates fibroblast proliferation as well as VEGF expression in hypertrophic scars (HS).

Methods: The expression of circ_0057452, miR-145-5p and TGF-β2 in HS tissues and fibroblasts was measured by quantitative real-time Polymerase Chain Reaction (qRT-PCR). The targeting relations between circ_0057452 and miR-145-5p, miR-145-5p and TGF-β2 were identified using dual-luciferase reporter assay. Read More

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Development and validation of a pregnancy prediction model based on ultrasonographic features related to endometrial receptivity.

Am J Transl Res 2021 15;13(6):6156-6165. Epub 2021 Jun 15.

Department of Ultrasound, Wenzhou People's Hospital Wenzhou, China.

Objective: Our aim was to identify multiple endometrial receptivity related factors by applying non-invasive, repeatable multimodal ultrasound methods. We further established a practical prediction model for pregnancy prediction.

Materials And Methods: Our study included 152 participants from Wenzhou People's Hospital, Wenzhou Maternal and Child Health Care Hospital, and the Third Affiliated Hospital of Wenzhou Medical University. Read More

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circRNA_103615 contributes to tumor progression and cisplatin resistance in NSCLC by regulating ABCB1.

Exp Ther Med 2021 Sep 1;22(3):934. Epub 2021 Jul 1.

Department of Oncology, Jinshazhou Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510168, P.R. China.

Lung cancer is one of the main causes of tumor lethality worldwide. Circular RNAs (circRNAs) have essential roles in tumor progression. However, in non-small cell lung carcinoma (NSCLC), the role of circRNAs remains unknown. Read More

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September 2021

Vaccination Against Diphtheria and Tetanus as a Way to Activate Adaptive Immunity in Children with Solid Tumors.

Front Immunol 2021 8;12:696816. Epub 2021 Jul 8.

Department of Allergology, I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.

Early studies on vaccination of children with oncological diseases were only dedicated to the assessment of safety and immunogenicity of the drug. Mechanisms of the post-vaccination immune response were not investigated. This study involved 41 patients aged 7-15 years who were treated for solid tumors two or more years ago. Read More

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