Int J Implant Dent 2021 Aug 4;7(1):70. Epub 2021 Aug 4.
Department of Dental Implantology, Yantai Stomatological Hospital Affiliated to Binzhou Medical College, No. 142, North Great Str, Yantai, Shandong, 264008, People's Republic of China.
Background: Concentrated growth factor (CGF) is a third-generation platelet concentrate product; the major source of growth factors in CGF is its extract; however, there are few studies on the overall effects of the extract of CGF (CGF-e). The aim of this study was to investigate the effect and mechanism of CGF-e on MC3T3-E1 cells in vitro and to explore the effect of combination of CGF-e and bone collagen (Bio-Oss Collagen, Geistlich, Switzerland) for bone formation in cranial defect model of rats in vivo.
Methods: The cell proliferation, ALP activity, mineral deposition, osteogenic-related gene, and protein expression were evaluated in vitro; the newly formed bone was evaluated by histological and immunohistochemical analysis through critical-sized cranial defect rat model in vivo. Read More