112,020 results match your criteria vivo decreased

Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats.

Bioelectromagnetics 2021 Jun 15. Epub 2021 Jun 15.

Rehabilitation Medicine Center, The First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.

Acute lung injury (ALI) features dysregulated pulmonary inflammation. Ultrashort waves (USWs) exert anti-inflammatory effects but no studies have evaluated their activity in ALI. Herein, we used an in vivo lipopolysaccharide (LPS)-induced ALI model to investigate whether the anti-inflammatory activity of USWs is mediated by altering the polarization of M1 to M2 macrophages. Read More

View Article and Full-Text PDF

Evaluation of lorcaserin as an anticonvulsant in juvenile Fmr1 knockout mice.

Epilepsy Res 2021 May 27;175:106677. Epub 2021 May 27.

Mercer University, College of Pharmacy, Department of Pharmaceutical Sciences, 3001 Mercer University Drive, Atlanta, GA, 30341, USA. Electronic address:

Recent preclinical and clinical studies suggest that lorcaserin, a preferential serotonin 2C receptor (5-HTR) agonist that was approved for the treatment of obesity, possesses antiepileptic properties. Here, we tested whether lorcaserin (1, 3, 5.6, 10 mg/kg) is prophylactic against audiogenic seizures (AGSs) in juvenile Fmr1 knockout mice, a mouse model of fragile X syndrome (FXS). Read More

View Article and Full-Text PDF

Cynaroside protects the blue light-induced retinal degeneration through alleviating apoptosis and inducing autophagy in vitro and in vivo.

Phytomedicine 2021 May 24;88:153604. Epub 2021 May 24.

State Key Laboratory of Natural Medicines, Department of TCMs Pharmaceuticals, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, People's Republic of China. Electronic address:

Background: Blue light can directly penetrate the lens and reach the retina to induce retinal damage, causing dry age-related macular degeneration (dAMD). Cynaroside (Cyn), a flavonoid glycoside, was proved to alleviate the oxidative damage of retinal cells in vitro. However, whether or not Cyn also exerts protective effect on blue light-induced retinal degeneration and its mechanisms of action are unclear. Read More

View Article and Full-Text PDF

Propofol represses cell growth and metastasis by modulating the circNCAPG/miR-200a-3p/RAB5A axis in glioma.

World Neurosurg 2021 Jun 12. Epub 2021 Jun 12.

Department of Anesthesiology, The First Hospital of Hebei Medicine University, Shijiazhuang, Hebei,050011, P.R. China. Electronic address:

Background: Glioma is a common primary intracranial tumor, with high infiltration and aggression. Propofol (Pro) is associated with growth and metastasis in glioma. Meanwhile, circular RNA non-SMC condensin I complex subunit G (circNCAPG, hsa_circ_0007244) has been reported to be upregulated in glioma. Read More

View Article and Full-Text PDF

In vivo bidirectional modulation of cannabinoid on the activity of globus pallidus in rats.

Neuroscience 2021 Jun 12. Epub 2021 Jun 12.

Department of Physiology and Pathophysiology, School of Basic Medicine, Qingdao University, Qingdao, China. Electronic address:

Endocannabinoids are bioactive substances which participate in central motor control. The globus pallidus (GP) is a major nucleus in the basal ganglia circuit, which plays an important function in movement regulation. Both cannabinoid receptor type 1 (CBR) and cannabinoid receptor type 2 (CBR) are expressed in the GP suggesting GP as a main action area of endocannabinoids. Read More

View Article and Full-Text PDF

Niujiaodihuang Detoxify Decoction inhibits ferroptosis by enhancing glutathione synthesis in acute liver failure models.

J Ethnopharmacol 2021 Jun 12:114305. Epub 2021 Jun 12.

School of Pharmacy,Guangdong Medical University,Dongguan 524023,China. Electronic address:

Ethnopharmacological Relevance: Niujiaodihuang Detoxify Decoction (NDD) is an integrated traditional Chinese medicine prescription that has been used as a therapeutic agent for the treatment of acute liver failure (ALF). However, the mechanisms underlying its action remain unclear.

Aim Of The Study: To determine the protective effect of NDD on D-galactosamine/lipopolysaccharide (D-GalN/LPS)-induced ALF and explore the underlying mechanisms. Read More

View Article and Full-Text PDF

Kaempferol inhibits benign prostatic hyperplasia by resisting the action of androgen.

Eur J Pharmacol 2021 Jun 12:174251. Epub 2021 Jun 12.

Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Poyang Lake Road 10, Tianjin 301617, China; Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Poyang Lake Road 10, Tianjin 301617, China; Laboratory of Pharmacology of TCM Formulae Co-Constructed By the Province-Ministry, Tianjin University of TCM, Poyang Lake Road 10, Tianjin 301617, China. Electronic address:

Kaempferol is a natural compound that inhibits tumor development in androgenic related prostate cancer. However, it is still not clear about its phyto-androgenic activity and whether it suppresses testosterone-induced benign prostatic hyperplasia (BPH) development. In this study, molecular docking, cellular immunofluorescence staining, chromatin immunoprecipitation and dual luciferase reporter assay were performed to investigate the androgenic activity of kaempferol. Read More

View Article and Full-Text PDF

Systemic administration of cytotoxic T lymphocyte-associated antigen 4 (CTLA-4)-Ig abrogates alveolar bone resorption in induced periodontitis through inhibition of osteoclast differentiation and activation: An experimental investigation.

J Periodontal Res 2021 Jun 15. Epub 2021 Jun 15.

Department of Periodontology, Tokyo Dental College, Tokyo, Japan.

Background/objectives: Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) is a critical immunoregulatory molecule expressed on T cells. CTLA-4 also binds to the surfaces of monocytes and macrophages, precursors of osteoclasts. Research on rheumatoid arthritis demonstrated that CTLA-4 suppresses inflammation and bone resorption. Read More

View Article and Full-Text PDF

MicroRNA-9 inhibits proliferation and progression in retinoblastoma cells by targeting PTEN.

Genes Genomics 2021 Jun 15. Epub 2021 Jun 15.

Department of Anesthesia Surgery, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, China.

Background: Retinoblastoma (RB) is the most prevalent primary intraocular malignancy, which commonly occurs during infant and childhood.

Objective: Our study aimed to investigate whether microRNA-9 (miR-9) could regulate RB cells and its mechanism.

Methods: qRT-PCR analysis was used to detect the expression of miR-9. Read More

View Article and Full-Text PDF

Vascular endothelial cell-derived exosomal miR-30a-5p inhibits lung adenocarcinoma malignant progression by targeting CCNE2.

Carcinogenesis 2021 Jun 15. Epub 2021 Jun 15.

Department of Oncology, The First Affiliated Hospital of Soochow University, Jiangsu, China, Department of Thoracic Surgery, Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Zhejiang Cancer Hospital, Hangzhou, Zhejiang, China, Institute of Cancer and Basic Medicine (IBMC) Chinese Academy of Sciences, Gongshu District, Hangzhou, China.

This study tried to explore the molecular mechanism underlying progression of lung adenocarcinoma (LUAD), and discuss the extracellular communication between cancer cells and vascular endothelial cells. Roughly, differential analysis was carried out to note that miR-30a-5p was lowly expressed in LUAD while CCNE2 was highly expressed. Cell functional experiments demonstrated that overexpressed miR-30a-5p led to suppressed cell abilities in proliferation, migration and invasion. Read More

View Article and Full-Text PDF

Tetrahydroxanthohumol, a xanthohumol derivative, attenuates high-fat diet-induced hepatic steatosis by antagonizing PPARγ.

Elife 2021 Jun 15;10. Epub 2021 Jun 15.

Linus Pauling Institute, Department of Biochemistry and Biophysics, Oregon State University, Corvallis, United States.

We previously reported xanthohumol (XN), and its synthetic derivative tetrahydro-XN (TXN), attenuates high-fat diet (HFD)-induced obesity and metabolic syndrome in C57Bl/6J mice. The objective of the current study was to determine the effect of XN and TXN on lipid accumulation in the liver. Non-supplemented mice were unable to adapt their caloric intake to 60% HFD, resulting in obesity and hepatic steatosis; however, TXN reduced weight gain and decreased hepatic steatosis. Read More

View Article and Full-Text PDF

Electroacupuncture alleviates spatial memory deficits in METH withdrawal mice by enhancing astrocyte-mediated glutamate clearance in the dCA1.

Addict Biol 2021 Jun 14:e13068. Epub 2021 Jun 14.

Department of Human Anatomy and Histoembryology, Nanjing University of Chinese Medicine, Nanjing, China.

Methamphetamine (METH) elicits endogenous glutamate (Glu) in the brain, which could partially explain METH-induced memory deficits. Here, we investigated the therapeutic effects of electroacupuncture (EA) on spatial memory deficits in METH withdrawal mice and its potential synaptic mechanisms. We found that EA at acupoints 'Baihui' and 'Yintang' ameliorated the impaired spatial memory in METH withdrawal mice. Read More

View Article and Full-Text PDF

Statins reduce castration-induced bone marrow adiposity and prostate cancer progression in bone.

Oncogene 2021 Jun 14. Epub 2021 Jun 14.

Department of Translational Molecular Pathology, University of Texas, MD Anderson Cancer Center, Houston, TX, USA.

A fraction of patients undergoing androgen deprivation therapy (ADT) for advanced prostate cancer (PCa) will develop recurrent castrate-resistant PCa (CRPC) in bone. Strategies to prevent CRPC relapse in bone are lacking. Here we show that the cholesterol-lowering drugs statins decrease castration-induced bone marrow adiposity in the tumor microenvironment and reduce PCa progression in bone. Read More

View Article and Full-Text PDF

Corticotropin-releasing factor neurons in the bed nucleus of the stria terminalis exhibit sex-specific pain encoding in mice.

Sci Rep 2021 Jun 14;11(1):12500. Epub 2021 Jun 14.

Department of Pharmacology, School of Medicine, University of North Carolina At Chapel Hill, CB 7178 Thurston Bowles Building, 104 Manning Drive, Chapel Hill, NC, 27599, USA.

The bed nucleus of the stria terminalis (BNST) plays an emerging role in pain regulation. Pharmacological studies have found that inhibiting corticotropin-releasing factor (CRF) signaling in the BNST can selectively mitigate the sensory and affective-motivational components of pain. However, mechanistic insight on the source of CRF that drives BNST responses to these harmful experiences remains unknown. Read More

View Article and Full-Text PDF

Macula Densa NOS1β Modulates Renal Hemodynamics and Blood Pressure During Pregnancy: Role in Gestational Hypertension.

J Am Soc Nephrol 2021 Jun 14. Epub 2021 Jun 14.

R Liu, Department of Molecular Pharmacology & Physiology, University of South Florida College of Medicine, Tampa, United States

Regulation of renal hemodynamics and blood pressure (BP) via tubuloglomerular feedback (TGF) may be an important adaptive mechanism during pregnancy. Because the β-splice variant of nitric oxide synthase 1 (NOS1β) in the macula densa is a primary modulator of TGF, we evaluated its role in normal pregnancy and gestational hypertension in a mouse model. We hypothesized that pregnancy upregulates NOS1β in the macula densa, thus blunting TGF, allowing glomerular filtration rate (GFR) to increase and BP to decrease. Read More

View Article and Full-Text PDF

Blood pressure and cardiac output during caesarean delivery under spinal anaesthesia: a prospective cohort study.

BMJ Open 2021 06 14;11(6):e046102. Epub 2021 Jun 14.

Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.

Objectives: We have previously established a method to measure transfer of nutrients between mother, placenta and fetus in vivo. The method includes measurements of maternal and fetal blood flow by Doppler ultrasound prior to spinal anaesthesia. Spinal anaesthesia affects maternal blood pressure and cardiac output. Read More

View Article and Full-Text PDF

In vitro effects of 2 bioactive compounds, gallic acid and methyl gallate, on urolithiasis.

Actas Urol Esp 2021 Jun 11. Epub 2021 Jun 11.

Programa de Pós-graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brasil. Electronic address:

Introduction And Objectives: This study aimed to evaluate the role of 2 widely distributed natural phenolic compounds, gallic acid (GA) and methyl gallate (MG), in an in vitro model of urolithiasis, by using the methodology of calcium oxalate crystals formation, which is the most common type of urinary or kidney stones.

Material And Methods: The compounds GA and MG were subjected to anti-crystallization activities in different concentrations (0.003-0. Read More

View Article and Full-Text PDF

Chitosan/gelatin-tannic acid decorated porous tape suture with multifunctionality for tendon healing.

Carbohydr Polym 2021 Sep 25;268:118246. Epub 2021 May 25.

Key Laboratory of Textile Science and Technology of Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology, Donghua University, Shanghai 201620, China. Electronic address:

The inferior tendon healing after surgery is inextricably linked to the surgical suture. Poor load transfer along the suture often results in a high tendon re-tear rate. Besides, the severe inflammation and infection induced by sutures even cause a second surgery. Read More

View Article and Full-Text PDF
September 2021

Antiviral effects of ergosterol peroxide in a pig model of porcine deltacoronavirus (PDCoV) infection involves modulation of apoptosis and tight junction in the small intestine.

Vet Res 2021 Jun 14;52(1):86. Epub 2021 Jun 14.

College of Veterinary Medicine, China Agricultural University, Beijing, China.

Porcine deltacoronavirus (PDCoV) is a newly discovered swine enteropathogenic coronavirus with worldwide distribution. However, efficient strategies to prevent or treat the infection remain elusive. Our in vitro study revealed that ergosterol peroxide (EP) from the mushroom Cryptoporus volvatus has efficient anti-PDCoV properties. Read More

View Article and Full-Text PDF

Clinical and experimental phenotype of azole-resistant Aspergillus fumigatus with a HapE splice site mutation: a case report.

BMC Infect Dis 2021 Jun 14;21(1):573. Epub 2021 Jun 14.

Department of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1, Sakamoto, Nagasaki, 852-8501, Japan.

Background: The recent increase in cases of azole-resistant Aspergillus fumigatus (ARAf) infections is a major clinical concern owing to its treatment limitations. Patient-derived ARAf occurs after prolonged azole treatment in patients with aspergillosis and involves various cyp51A point mutations or non-cyp51A mutations. The prognosis of patients with chronic pulmonary aspergillosis (CPA) with patient-derived ARAf infection remains unclear. Read More

View Article and Full-Text PDF

Roles of Aryl-hydrocarbon Receptor in metabolism and proliferative behaviors of Bone Marrow MSCs: Implications for targeted therapy.

Curr Top Med Chem 2021 Jun 11. Epub 2021 Jun 11.

Department of Hematology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.

Background: Mesenchymal stromal cells (MSCs) due to convenient isolation and ex vivo expansion, multipotent differentiation, and migrating capacity into the inflammation sites and tumor microenvironments are interesting candidates in precise medicine. MSCs, as an important component of bone marrow microenvironment, can regulate hematopoiesis and immunity via direct contact or secretion of adhesion factors and hematopoietic chemokines. Recent evidence showed that aryl-hydrocarbon receptor (AHR) signal pathway plays a crucial role in hematopoiesis and immunity. Read More

View Article and Full-Text PDF

The local anesthetic ropivacaine suppresses progression of breast cancer by regulating miR-27b-3p/YAP axis.

Aging (Albany NY) 2021 Jun 14;13. Epub 2021 Jun 14.

The Department of Anesthesiology, Handan Central Hospital, Handan, China.

Breast cancer is a prevalent malignancy with high mortality and poor prognosis. Ropivacaine is a widely used local anesthetic and presents potential anti-tumor activity. Nevertheless, the function of ropivacaine in breast cancer development remains elusive. Read More

View Article and Full-Text PDF

Cardiac fibrosis models using human induced pluripotent stem cell-derived cardiac tissues allow anti-fibrotic drug screening in vitro.

Stem Cell Res 2021 Jun 11;54:102420. Epub 2021 Jun 11.

Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Yamadaoka, 2-2, Suita, Osaka 565-0871, Japan. Electronic address:

Drug efficacy assessment without using animals is important for development of cardiac fibrosis treatment. In this study, potential anti-fibrotic drugs were screened in a model of diseased myocardium using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) and non-CM in in vitro and in vivo heart failure models. Cardiomyogenic differentiation was induced in hiPSC to generate cardiac tissue, including both iPSC-CM and non-CM expressing fibroblast markers. Read More

View Article and Full-Text PDF

Schisantherin A ameliorates liver fibrosis through TGF-β1mediated activation of TAK1/MAPK and NF-κB pathways in vitro and in vivo.

Phytomedicine 2021 May 24;88:153609. Epub 2021 May 24.

Department of Pharmacology, College of Pharmacy, Beihua University, No. 3999 Binjiang East Road, Jilin, Jilin Province, 132013, China. Electronic address:

Backgroud: Schisandra chinensis, a traditional Chinese medicine for liver protection, can significantly improve liver fibrosis. However, it is still unclear which active components in Schisandra chinensis play an anti-fibrosis role.

Purpose: The purpose of present study was to observe the anti-fibrosis effect of schisantherin A (SCA) on liver fibrosis and explore its underlying mechanism. Read More

View Article and Full-Text PDF

Effect of dietary supplementation with nettle or fenugreek on folliculogenesis and steroidogenesis in the rabbit ovary - An in vivo study.

Theriogenology 2021 Jun 8;173:1-11. Epub 2021 Jun 8.

Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059, Krakow, Poland.

The objective of the study was to investigate the effect of dietary supplementation with nettle or fenugreek on folliculogenesis and steroidogenesis in the juvenile rabbit ovary. To gain insight into the mechanism of action of these herbs, we examined follicle formation, ovarian cell proliferation and apoptosis, steroidogenic enzyme abundance and steroid concentrations in ovarian tissue and plasma. Animals were fed with control, 1% nettle- or 1% fenugreek-supplemented pellets from 5 to 12 weeks of age (n = 10 per each group), when animals were slaughtered for ovary and blood collection. Read More

View Article and Full-Text PDF

The protective effect of HOXA5 on carotid atherosclerosis occurs by modulating the vascular smooth muscle cell phenotype.

Mol Cell Endocrinol 2021 Jun 11:111366. Epub 2021 Jun 11.

Department of Vascular Surgery, The First Hospital of China Medical University, Shenyang, China. Electronic address:

The phenotypic change of vascular smooth muscle cells (VSMCs) from a contractile to a synthetic form is a key player in atherogenic processes. Homeobox A5 (HOXA5), a transcription factor of the homeobox gene family, has been shown to regulate cell differentiation and morphogenesis. The present study was designed to clarify the involvement of HOXA5 in VSMC phenotypic transition in carotid atherosclerosis (CAS). Read More

View Article and Full-Text PDF

Upregulation of RNA cap methyltransferase RNMT drives ribosome biogenesis during T cell activation.

Nucleic Acids Res 2021 Jun 14. Epub 2021 Jun 14.

Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.

The m7G cap is ubiquitous on RNAPII-transcribed RNA and has fundamental roles in eukaryotic gene expression, however its in vivo role in mammals has remained unknown. Here, we identified the m7G cap methyltransferase, RNMT, as a key mediator of T cell activation, which specifically regulates ribosome production. During T cell activation, induction of mRNA expression and ribosome biogenesis drives metabolic reprogramming, rapid proliferation and differentiation generating effector populations. Read More

View Article and Full-Text PDF

Punicalagin ameliorates collagen-induced arthritis by downregulating M1 macrophage and pyroptosis via NF-κB signaling pathway.

Sci China Life Sci 2021 Jun 10. Epub 2021 Jun 10.

Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.

Rheumatoid arthritis (RA) is a chronic inflammatory disease that eventually leads to disability. Inflammatory cell infiltration, severe joint breaking and systemic bone loss are the main clinical symptoms. In this study, we established a collagen-induced arthritis (CIA) model and found a large number of M1 macrophages and pyroptosis, which are important sources of proinflammatory cytokines. Read More

View Article and Full-Text PDF

Histidine ameliorates elastase- and lipopolysaccharide-induced lung inflammation by inhibiting the activation of the NLRP3 inflammasome.

Acta Biochim Biophys Sin (Shanghai) 2021 Jun 14. Epub 2021 Jun 14.

Department of Respiratory Medicine, Peking University Third Hospital, Beijing 100191, China.

Histidine treatment has anti-inflammatory effects on several diseases such as colitis and obesity. We revealed that histidine levels were decreased in the serum of patients with chronic obstructive pulmonary disease (COPD) in our previous study. However, whether histidine confers protection against COPD is unclear. Read More

View Article and Full-Text PDF

Tyrosine-Protein Phosphatase Non-receptor Type 9 (PTPN9) Negatively Regulates the Paracrine Vasoprotective Activity of Bone-Marrow Derived Pro-angiogenic Cells: Impact on Vascular Degeneration in Oxygen-Induced Retinopathy.

Front Cell Dev Biol 2021 28;9:679906. Epub 2021 May 28.

Department of Ophthalmology, Maisonneuve-Rosemont Hospital Research Center, University of Montréal, Montréal, QC, Canada.

Background And Aim: Insufficient post-ischemic neovascularization is an initial key step in the pathogenesis of Oxygen-Induced Retinopathy (OIR). During neovascularization, pro-angiogenic cells (PACs) are mobilized from the bone marrow and integrate into ischemic tissues to promote angiogenesis. However, the modulation of PAC paracrine activity during OIR and the specific mechanisms involved remain to be explored. Read More

View Article and Full-Text PDF