5,524 results match your criteria vitro mobilization


Surface Modification with NGF-Loaded Chitosan/Heparin Nanoparticles for Improving Biocompatibility of Cardiovascular Stent.

Stem Cells Int 2021 24;2021:9941143. Epub 2021 Apr 24.

Department of Rehabilitation, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, 310000 Zhejiang Province, China.

Late thrombosis and restenosis remain major challenges to the safety of drug-eluting stents. Biofunctional modification to endow the surface with selective anticoagulation and promote endothelium regeneration has become a hotpot recently. In this study, chitosan and heparin were found to form three-dimensional nanoparticles by spontaneous electrostatic interaction. Read More

View Article and Full-Text PDF

NK Cells and PMN-MDSCs in the Graft From G-CSF Mobilized Haploidentical Donors Display Distinct Gene Expression Profiles From Those of the Non-Mobilized Counterpart.

Front Immunol 2021 27;12:657329. Epub 2021 Apr 27.

Immunology Research Area, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

A recent approach of hematopoietic stem cell (HSC) transplantation from haploidentical donors "mobilized" with G-CSF is based on the selective depletion of T and B lymphocytes from the graft. Through this approach, the patient receives both HSC and mature donor-derived effector cells (including NK cells), which exert both anti-leukemia activity and protection against infections. We previously showed that donor HSC mobilization with G-CSF results in accumulation in the graft of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs), capable of inhibiting the anti-leukemia activity of allogeneic NK cells. Read More

View Article and Full-Text PDF

Biased action of the CXCR4-targeting drug plerixafor is essential for its superior hematopoietic stem cell mobilization.

Commun Biol 2021 May 12;4(1):569. Epub 2021 May 12.

Department of Biomedical Sciences, Faculty of Health and Medical Sciences, The Panum Institute, University of Copenhagen, Copenhagen, Denmark.

Following the FDA-approval of the hematopoietic stem cell (HSC) mobilizer plerixafor, orally available and potent CXCR4 antagonists were pursued. One such proposition was AMD11070, which was orally active and had superior antagonism in vitro; however, it did not appear as effective for HSC mobilization in vivo. Here we show that while AMD11070 acts as a full antagonist, plerixafor acts biased by stimulating β-arrestin recruitment while fully antagonizing G protein. Read More

View Article and Full-Text PDF

Allyl Isothiocyanate: A TAS2R38 Receptor-Dependent Immune Modulator at the Interface Between Personalized Medicine and Nutrition.

Front Immunol 2021 20;12:669005. Epub 2021 Apr 20.

Molecular Preventive Medicine, University Medical Center and Faculty of Medicine-University of Freiburg, Freiburg, Germany.

Understanding individual responses to nutrition and medicine is of growing interest and importance. There is evidence that differences in bitter taste receptor (TAS2R) genes which give rise to two frequent haplotypes, TAS2R38-PAV (functional) and TAS2R38-AVI (non-functional), may impact inter-individual differences in health status. We here analyzed the relevance of the TAS2R38 receptor in the regulation of the human immune response using the TAS2R38 agonist allyl isothiocyanate (AITC) from plants. Read More

View Article and Full-Text PDF

Antiplatelet and Antithrombotic Effects of Nakai.

Evid Based Complement Alternat Med 2021 16;2021:7071987. Epub 2021 Apr 16.

Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.

. Nakai is a medicinal plant known for its health beneficial effects on impotence, arrhythmia, oxidation, aging, osteoporosis, and cardiovascular diseases. However, there is no report available that shows its effects on platelet functions. Read More

View Article and Full-Text PDF

Evolving origin-of-transfer sequences on staphylococcal conjugative and mobilizable plasmids-who's mimicking whom?

Nucleic Acids Res 2021 May 3. Epub 2021 May 3.

Curtin Health Innovation Research Institute, Curtin University, Perth, WA 6102, Australia.

In Staphylococcus aureus, most multiresistance plasmids lack conjugation or mobilization genes for horizontal transfer. However, most are mobilizable due to carriage of origin-of-transfer (oriT) sequences mimicking those of conjugative plasmids related to pWBG749. pWBG749-family plasmids have diverged to carry five distinct oriT subtypes and non-conjugative plasmids have been identified that contain mimics of each. Read More

View Article and Full-Text PDF

Pharmacological characterization of naloxegol: In vitro and in vivo studies.

Eur J Pharmacol 2021 Apr 30;903:174132. Epub 2021 Apr 30.

Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Italy.

Opioid-induced constipation is the most prevalent adverse effect of opioid drugs. Peripherally acting mu opioid receptor antagonists (PAMORAs), including naloxegol, are indicated for the treatment of opioid-induced constipation. The aim of this study was the in vitro and in vivo pharmacological characterization of naloxegol in comparison with naloxone. Read More

View Article and Full-Text PDF

Furin Prodomain ppFurin Enhances Ca Entry Through Orai and TRPC6 Channels' Activation in Breast Cancer Cells.

Cancers (Basel) 2021 Apr 1;13(7). Epub 2021 Apr 1.

LAMC, INSERM, University Bordeaux, U1029, F-33600 Pessac, France.

The intracellular calcium concentration ([Ca]) modulation plays a key role in the regulation of cellular growth and survival in normal cells and failure of [Ca] homeostasis is involved in tumor initiation and progression. Here we showed that inhibition of Furin by its naturally occurring inhibitor the prodomain ppFurin in the MDA-MB-231 breast cancer cells resulted in enhanced store-operated calcium entry (SOCE) and reduced the cell malignant phenotype. Expression of ppFurin in a stable manner in MDA-MB-231 and the melanoma MDA-MB-435 cell lines inhibits Furin activity as assessed by in vitro digestion assays. Read More

View Article and Full-Text PDF

Biological characterization of ligands targeting the human CC chemokine receptor 8 (CCR8) reveals the biased signaling properties of small molecule agonists.

Biochem Pharmacol 2021 Apr 17;188:114565. Epub 2021 Apr 17.

KU Leuven, Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, Herestraat 49, 3000 Leuven, Belgium. Electronic address:

The human CC chemokine receptor 8 (CCR8) is a promising drug target for cancer immunotherapy and autoimmune disease. Besides human and viral chemokines, previous studies revealed diverse classes of CCR8-targeting small molecules. We characterized a selection of these CCR8 ligands (hCCL1, vCCL1, ZK756326, AZ6; CCR8 agonists and a naphthalene-sulfonamide-based CCR8 antagonist), in in vitro cell-based assays (hCCL1 binding, calcium mobilization, cellular impedance, cell migration, β-arrestin 1/2 recruitment), and used pharmacological tools to determine G protein-dependent and -independent signaling pathways elicited by these ligands. Read More

View Article and Full-Text PDF

Investigation of the Structure-Activity Relationships of Psilocybin Analogues.

ACS Pharmacol Transl Sci 2021 Apr 14;4(2):533-542. Epub 2020 Dec 14.

Department of Psychiatry, University of California San Diego, La Jolla, California 92093-0804, United States.

The 5-HT receptor is thought to be the primary target for psilocybin (4-phosphoryloxy-,-dimethyltryptamine) and other serotonergic hallucinogens (psychedelic drugs). Although a large amount of experimental work has been conducted to characterize the pharmacology of psilocybin and its dephosphorylated metabolite psilocin (4-hydroxy-,-dimethyltryptamine), there has been little systematic investigation of the structure-activity relationships (SAR) of 4-substituted tryptamine derivatives. In addition, structural analogs of psilocybin containing a 4-acetoxy group, such as 4-acetoxy-,-dimethyltryptamine (4-AcO-DMT), have appeared as new designer drugs, but almost nothing is known about their pharmacological effects. Read More

View Article and Full-Text PDF

Structure-Activity Relationship Analysis of Psychedelics in a Rat Model of Asthma Reveals the Anti-Inflammatory Pharmacophore.

ACS Pharmacol Transl Sci 2021 Apr 13;4(2):488-502. Epub 2020 Aug 13.

Department of Pharmacology and Experimental Therapeutics, Louisiana Stat University Health Sciences Center, New Orleans, Louisiana 70112, United States.

Psychedelic drugs can exert potent anti-inflammatory effects. However, anti-inflammatory effects do not appear to correlate with behavioral activity, suggesting different underlying mechanisms. We hypothesized that the distinct structural features of psychedelics underlie functionally selective mechanisms at the target 5-HT receptor to elicit maximal anti-inflammatory effects. Read More

View Article and Full-Text PDF

Enhanced phytoextraction of multi-metal contaminated soils under increased atmospheric temperature by bioaugmentation with plant growth promoting Bacillus cereus.

J Environ Manage 2021 Jul 12;289:112553. Epub 2021 Apr 12.

Department of Environmental Sciences, Bharathiar University, Coimbatore, 641046, India. Electronic address:

The co-occurrence of environmental stresses such as heavy metals (HM) and increased atmospheric temperature (IAT) pose serious implications on plant growth and productivity. In this work, we evaluated the role of plant growth-promoting bacteria (PGPB) and its effectiveness on Zea mays growth, stress tolerance and phytoremediation potential in multi-metal (MM) contaminated soils under IAT stress conditions. The PGPB strain TCU11 was isolated from metal contaminated soils and identified as Bacillus cereus. Read More

View Article and Full-Text PDF

"Photo-Rimonabant": Synthesis and Biological Evaluation of Novel Photoswitchable Molecules Derived from Rimonabant Lead to a Highly Selective and Nanomolar "-On" CBR Antagonist.

ACS Chem Neurosci 2021 May 15;12(9):1632-1647. Epub 2021 Apr 15.

Pharmazeutische und Medizinische Chemie, Institut für Pharmazie und Lebensmittelchemie, Julius-Maximilians-Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany.

Human cannabinoid receptor type 1 (CBR) plays important roles in the regulation of appetite and development of addictive behaviors. Herein, we describe the design, synthesis, photocharacterization, molecular docking, and characterization of "photo-rimonabant", i.e. Read More

View Article and Full-Text PDF

Blueprint for pandemic response: Focus on translational medicine, clinical pharmacology and pharmacometrics.

Br J Clin Pharmacol 2021 Apr 14. Epub 2021 Apr 14.

Certara, Princeton, NJ, USA.

Perhaps the most important lesson learned from the COVID-19 pandemic is that of preparedness. Enhanced surveillance systems for early threat detection will be crucial to maximizing response time for implementation of public health measures and mobilization of resources in containing an emerging pandemic. Recent outbreaks have been dominated by viral pathogens, with RNA respiratory viruses being the most likely to have pandemic potential. Read More

View Article and Full-Text PDF

Carbon Monoxide Suppresses Neointima Formation in Transplant Arteriosclerosis by Inhibiting Vascular Progenitor Cell Differentiation.

Arterioscler Thromb Vasc Biol 2021 Apr 15:ATVBAHA120315558. Epub 2021 Apr 15.

Department of Surgery, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA (H.S., G.R.L., E.C., D.G., H.W., F.H.B., L.E.O.).

Objective: Evidence indicates that bone marrow progenitor cells (BMPC) are a major contributor to neointima formation in transplant arteriosclerosis. HO-1 (heme oxygenase 1, ) and carbon monoxide (CO), a product of heme degradation by HO-1, ameliorate neointima formation by inhibiting proliferation of smooth muscle cells. We investigated the mechanism whereby HO-1 and CO modulate BMPC and mitigates neointima formation in transplant arteriosclerosis. Read More

View Article and Full-Text PDF

Inhibition of CXCR4 ameliorates hypoxia-induced pulmonary arterial hypertension in rats.

Am J Transl Res 2021 15;13(3):1458-1470. Epub 2021 Mar 15.

Department of Anesthesiology, The Affiliated Wuxi People's Hospital of Nanjing Medical University Wuxi, Jiangsu Province, China.

Pulmonary vascular remodeling due to aberrant proliferation and migration of pulmonary artery smooth muscle cells (PASMCs) is the main characteristic of pulmonary arterial hypertension (PAH). CXCR4 is a specific stem cell surface receptor of cytokine CXCL12 which could regulate homing of hematopoietic progenitor cells and their mobilization. There is evidence that bone marrow-derived CXCR4 proangiogenic cell accumulation take an important part in the development of pulmonary arterial hypertension; however, the underlying mechanisms still remain unknown. Read More

View Article and Full-Text PDF

Systems Approach to Discovery of Therapeutic Targets for Vein Graft Disease PPARα Pivotally Regulates Metabolism, Activation, and Heterogeneity of Macrophages and Lesion Development.

Circulation 2021 Apr 6. Epub 2021 Apr 6.

Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA; Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA; Center for Excellence in Vascular Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA; Department of Human Pathology, I.M. Sechenov First Moscow State Medical University of the Ministry of Health, Moscow, Russian Federation.

Vein graft failure remains a common clinical challenge. We applied a systems approach in mouse experiments to discovering therapeutic targets for vein graft failure. Global proteomics and high-dimensional clustering on multiple vein graft tissues were used to identify potential pathogenic mechanisms. Read More

View Article and Full-Text PDF

Distinct Expression Patterns of Cxcl12 in Mesenchymal Stem Cell Niches of Intact and Injured Rodent Teeth.

Int J Mol Sci 2021 Mar 16;22(6). Epub 2021 Mar 16.

Orofacial Development and Regeneration, Institute of Oral Biology, Medical Faculty, University of Zurich, 8032 Zurich, Switzerland.

Specific stem cell populations within dental mesenchymal tissues guarantee tooth homeostasis and regeneration throughout life. The decision between renewal and differentiation of stem cells is greatly influenced by interactions with stromal cells and extracellular matrix molecules that form the tissue specific stem cell niches. The chemokine is a general marker of stromal cells and plays fundamental roles in the maintenance, mobilization and migration of stem cells. Read More

View Article and Full-Text PDF

Quantitative Proteomics Analysis of Berberine-Treated Colon Cancer Cells Reveals Potential Therapy Targets.

Biology (Basel) 2021 Mar 23;10(3). Epub 2021 Mar 23.

College of Life Sciences, Northwest University, Xi'an 710069, China.

Colon cancer is one of the most lethal malignancies worldwide. Berberine has been found to exert potential anti-colon cancer activity in vitro and in vivo, although the detailed regulatory mechanism is still unclear. This study aims to identify the underlying crucial proteins and regulatory networks associated with berberine treatment of colon cancer by using proteomics as well as publicly available transcriptomics and tissue array data. Read More

View Article and Full-Text PDF

RANK promotes colorectal cancer migration and invasion by activating the Ca-calcineurin/NFATC1-ACP5 axis.

Cell Death Dis 2021 Apr 1;12(4):336. Epub 2021 Apr 1.

Scientific Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China.

The tumor necrosis factor (TNF) receptor superfamily member 11a (TNFRSF11a, also known as RANK) was demonstrated to play an important role in tumor metastasis. However, the specific function of RANK in colorectal cancer (CRC) metastasis and the underlying mechanism are unknown. In this study, we found that RANK expression was markedly upregulated in CRC tissues compared with that in matched noncancerous tissues. Read More

View Article and Full-Text PDF

Intra-articular Injection of PDGF-BB Explored in a Novel in Vitro Model Mobilizes Mesenchymal Stem Cells From the Synovium Into Synovial Fluid in Rats.

Stem Cell Rev Rep 2021 Mar 26. Epub 2021 Mar 26.

Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, 113-8510, Tokyo, Japan.

Background: Drugs that can induce mesenchymal stem cell (MSC) mobilization from synovium into synovial fluid will enable regenerative medicine in joints without use of exogenous MSCs. An in vitro synovial MSC migration model had previously been developed for screening but had problems in practical application. We herein developed a novel in vitro model, explored cytokines for synovial MSC mobilization with this model, and verified whether MSCs in synovial fluid increase following intra-articular injection of the cytokine. Read More

View Article and Full-Text PDF

Chronic restraint stress promotes the mobilization and recruitment of myeloid-derived suppressor cells through β-adrenergic-activated CXCL5-CXCR2-Erk signaling cascades.

Int J Cancer 2021 Mar 9. Epub 2021 Mar 9.

Department of Pharmacology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College, Beijing, China.

Myeloid-derived suppressor cells (MDSCs) play an important role in tumor immune escape. Recent studies have shown that MDSCs contribute to tumor progression under psychological stress, but the underlying mechanism of MDSCs mobilization and recruitment remains largely unknown. In the present study, a chronic restraint stress paradigm was applied to the H22 hepatocellular carcinoma (HCC) bearing mice to mimic the psychological stress. Read More

View Article and Full-Text PDF

The Combined Contribution of Vascular Endothelial Cell Migration and Adhesion to Stent Re-endothelialization.

Front Cell Dev Biol 2021 4;9:641382. Epub 2021 Mar 4.

West China School of Basic Medical Sciences and Forensic Medicine, Institute of Biomedical Engineering, Sichuan University, Chengdu, China.

Coronary stent placement inevitably causes mechanical damage to the endothelium, leading to endothelial denudation and in-stent restenosis (ISR). Re-endothelialization depends mainly on the migration of vascular endothelial cells (VECs) adjacent to the damaged intima, as well as the mobilization and adhesion of circulating VECs. To evaluate the combined contribution of VEC migration and adhesion to re-endothelialization under flow and the influence of stent, models were constructed to simulate various endothelial denudation scales (2 mm/5 mm/10 mm) and stent deployment depths (flat/groove/bulge). Read More

View Article and Full-Text PDF

Effect of memantine, an anti-Alzheimer's drug, on rodent microglial cells in vitro.

Sci Rep 2021 Mar 17;11(1):6151. Epub 2021 Mar 17.

Department of Psychiatry, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan.

The pathophysiology of Alzheimer's disease (AD) is related to neuroinflammatory responses mediated by microglia. Memantine, an antagonist of N-methyl-D-aspartate (NMDA) receptors used as an anti-Alzheimer's drug, protects from neuronal death accompanied by suppression of proliferation and activation of microglial cells in animal models of AD. However, it remains to be tested whether memantine can directly affect microglial cell function. Read More

View Article and Full-Text PDF

Natural killer T cell immunotherapy combined with oncolytic vesicular stomatitis virus or reovirus treatments differentially increases survival in mouse models of ovarian and breast cancer metastasis.

J Immunother Cancer 2021 Mar;9(3)

Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada

Background: Oncolytic viruses reduce tumor burden in animal models and have generated promising results in clinical trials. However, it is likely that oncolytic viruses will be more effective when used in combination with other therapies. Current therapeutic approaches, including chemotherapeutics, come with dose-limiting toxicities. Read More

View Article and Full-Text PDF

Cement augmentation for trochanteric fracture in elderly: A systematic review.

J Clin Orthop Trauma 2021 Apr 20;15:65-70. Epub 2020 Oct 20.

Department of Orthopaedic Surgery (DICHIRONS), University of Palermo, Palermo, Italy.

Background: Cement augmentation of internal fixation of hip fracture has reported to improve fracture stability in osteoporotic hip fractures, reducing the risk of cut-out of the sliding screw through the femoral head. The purpose of present study was to perform a systematic literature review on the effects of augmentation technique in patients with osteoporotic hip fractures.

Material And Methods: A comprehensive literature search was systematically performed to evaluate all papers published in English language included in the literature between January 2010 and July 2020, according to the PRISMA 2009 guidelines. Read More

View Article and Full-Text PDF

Submission for Special Issue: The Role of Platelet Activation in the Pathophysiology of HIV, Tuberculosis, and Pneumococcal Disease. Bedaquiline Suppresses ADP-Mediated Activation of Human Platelets Interference With Phosphatidylinositol 3-Kinase.

Front Immunol 2020 26;11:621148. Epub 2021 Feb 26.

Department of Immunology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

Although bedaquiline has advanced the treatment of multidrug-resistant tuberculosis (TB), concerns remain about the cardiotoxic potential of this agent, albeit by unexplored mechanisms. Accordingly, we have investigated augmentation of the reactivity of human platelets as a potential mechanism of bedaquiline-mediated cardiotoxicity. Platelet-rich plasma (PRP) or isolated cells prepared from the blood of healthy, adult humans were treated with bedaquiline (0. Read More

View Article and Full-Text PDF
February 2021

Circulating extracellular vesicles induce monocyte dysfunction and are associated with sepsis and high mortality in cirrhosis.

Liver Int 2021 Mar 13. Epub 2021 Mar 13.

Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India.

Background: Sepsis is common in cirrhosis and is often a result of immune dysregulation. Specific stimuli and pathways of inter-cellular communications between immune cells in cirrhosis and sepsis are incompletely understood. Immune cell-derived extracellular vesicles (EV) were studied to understand mechanisms of sepsis in cirrhosis. Read More

View Article and Full-Text PDF

Anti-thrombotic effects mediated by dihydromyricetin involve both platelet inhibition and endothelial protection.

Pharmacol Res 2021 May 10;167:105540. Epub 2021 Mar 10.

Department of Pharmacology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; The Key Laboratory for Drug Target Researches and Pharmacodynamic Evaluation of Hubei Province, Hubei 430030, China. Electronic address:

Classical antithrombotics and antiplatelets are associated with high frequencies of bleeding complications or treatment failure when used as single agents. The platelet-independent fibrin generation by activated endothelium highlights the importance of vascular protection in addition to platelet inhibition in thrombosis prevention. Dihydromyricetin (DHM), the most abundant flavonoid in Ampelopsis grossedentata, has unique vasoprotective effects. Read More

View Article and Full-Text PDF