1,569,550 results match your criteria vitro

Dl-3-n-butylphthalide pretreatment attenuates renal ischemia/reperfusion injury.

Biochem Biophys Res Commun 2021 Apr 14;557:166-173. Epub 2021 Apr 14.

Department of Nephrology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China. Electronic address:

Background: Renal ischemia reperfusion injury (IRI) has become a growing concern in clinical practice with high morbidity and mortality rates. There is currently no effective prophylactic regimen available to prevent its occurrence and to improve its clinical prognosis. Dl-3-n-butylphthalide (NBP) has been used for stroke treatment in China for years. Read More

View Article and Full-Text PDF

TRIM27 protects against cardiac ischemia-reperfusion injury by suppression of apoptosis and inflammation via negatively regulating p53.

Biochem Biophys Res Commun 2021 Apr 14;557:127-134. Epub 2021 Apr 14.

Department of General Medicine, The Sixth Annex Hospital of Xinjiang Medical University, Wulumuqi, 830002, China. Electronic address:

Myocardial ischemia/reperfusion (MI/R) has high morbidity and mortality worldwide, but the underlying mechanisms have not been entirely understood. TRIM27 is one of the Tri-domain proteins (TRIM) family proteins with crucial roles in numerous life processes. In the study, we attempted to explore the effects of heart-conditional knockout of TRIM27 (TRIM27) on MI/R progression both in vivo and in vitro. Read More

View Article and Full-Text PDF

Placenta pathology in recipient versus donor oocyte derivation for in vitro fertilization in a setting of hypertensive disorders of pregnancy and IUGR.

Placenta 2021 Mar 23;108:114-121. Epub 2021 Mar 23.

Department of Pathology, University of Ottawa, Children's Hospital of Eastern Ontario, 401 Smyth Rd, Ottawa, ON K1H 8L1, Canada. Electronic address:

Introduction: Assisted reproductive technology including in vitro fertilization (IVF) and oocyte donation (OD) may increase risk for placenta-mediated diseases. Comprehensive analysis of histopathological placental lesions according to source of oocytes used in the IVF procedure - recipient derived (RD-IVF) vs oocyte donation (OD-IVF), has not been conducted in a population with a hypertensive disorder of pregnancy (HDP) and/or intrauterine growth restriction (IUGR).

Methods: A retrospective cohort study of archived placenta specimens from RD-IVF and OD-IVF pregnancies affected by HDP and/or IUGR was conducted with blinded histopathological placental examination. Read More

View Article and Full-Text PDF

A general strategy to synthesis chitosan oligosaccharide-O-Terpenol derivatives with antibacterial properties.

Carbohydr Res 2021 Apr 14;503:108315. Epub 2021 Apr 14.

State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122, PR China; School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122, PR China; International Joint Laboratory on Food Safety, Jiangnan University, Lihu Road 1800, Wuxi, 214122, PR China; Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Lihu Road 1800, Wuxi, 214122, PR China.

The objectives of the present study are to synthesize a series of chitosan oligosaccharide-O-Terpenol (COS-O-Ter) derivatives and their implication to evaluate in vitro antibacterial activity. Herein, a general strategy is described for preparing COS-O-Ter derivatives, including substitution and deprotection reactions. The structures of COS-O-Ter derivatives were characterized by FT-IR, H NMR, XRD, TGA, and elemental analysis. Read More

View Article and Full-Text PDF

An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual.

Stem Cell Res 2021 Apr 8;53:102336. Epub 2021 Apr 8.

Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Key Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, Hunan 410008, China. Electronic address:

We established an induced pluripotent stem cell (iPSC) reprogrammed from dermal fibroblasts of a 53-year-old healthy man. Retroviruses expressing Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) were employed to initiate the reprogramming and were silenced in iPSCs. The generated iPSCs were pluripotent as determined by immunostaining and expression of pluripotency markers. Read More

View Article and Full-Text PDF

Metabolic shifts modulate lung injury caused by infection with H1N1 influenza A virus.

Virology 2021 Apr 6;559:111-119. Epub 2021 Apr 6.

Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA. Electronic address:

Influenza A virus (IAV) infection alters lung epithelial cell metabolism in vitro by promoting a glycolytic shift. We hypothesized that this shift benefits the virus rather than the host and that inhibition of glycolysis would improve infection outcomes. A/WSN/33 IAV-inoculated C57BL/6 mice were treated daily from 1 day post-inoculation (d. Read More

View Article and Full-Text PDF

Design, synthesis, biological evaluation, and computational studies of novel thiazolo-pyrazole hybrids as promising selective COX-2 inhibitors: Implementation of apoptotic genes expression for ulcerogenic liability assessment.

Bioorg Chem 2021 Mar 31;111:104883. Epub 2021 Mar 31.

Department of Organic and Medicinal Chemistry, Faculty of Pharmacy, University of Sadat City, Menoufiya 32897 Egypt.

A novel series of thiazolo-pyrazole hybrids has been prepared and assessed for their in vitro COX-1/COX-2 inhibitory activity. Compound 6c exhibited the most selective COX-2 inhibition profile (SI of 264) not far of Celecoxib (294). In-vivo anti-inflammatory activity revealed that compound 6d exhibited the highest activity (97. Read More

View Article and Full-Text PDF

Synthesis and evaluation of N-(4-(substituted)-3-(trifluoromethyl) phenyl) isobutyramides and their N-ethyl analogous as anticancer, anti-angiogenic & antioxidant agents: In vitro and in silico analysis.

Comput Biol Chem 2021 Apr 8;92:107484. Epub 2021 Apr 8.

Department of Biotechnology, Savitribai Phule Pune University, Pune, 411007, MS, India. Electronic address:

N-(4-(substituted)-3-(trifluoromethyl) phenyl) isobutyramides and their N-ethyl analogues (flutamides) are versatile scaffolds with a wide spectrum of biological activities. A series of new N-(4-(substituted)-3-(trifluoromethyl) phenyl) isobutyramides (8a-t) and their N-ethyl analogous (9a-t) were synthesized and characterized. The inhibitory potential of the synthesized compounds on the viability of three human cancer cell lines HEP3BPN 11 (liver), MDA-MB 453 (breast), and HL 60 (leukemia) were assessed. Read More

View Article and Full-Text PDF

Bioengineered microfluidic blood-brain barrier models in oncology research.

Transl Oncol 2021 Apr 14;14(7):101087. Epub 2021 Apr 14.

Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, 2713 Doha, Qatar; Biomedical Research Center (BRC), Qatar University, PO Box 2713 Doha, Qatar. Electronic address:

Metastasis is the major reason for most brain tumors with up to a 50% chance of occurrence in patients with other types of malignancies. Brain metastasis occurs if cancer cells succeed to cross the 'blood-brain barrier' (BBB). Moreover, changes in the structure and function of BBB can lead to the onset and progression of diseases including neurological disorders and brain-metastases. Read More

View Article and Full-Text PDF

Melatonin rescues cerebral ischemic events through upregulated tunneling nanotube-mediated mitochondrial transfer and downregulated mitochondrial oxidative stress in rat brain.

Biomed Pharmacother 2021 Apr 14;139:111593. Epub 2021 Apr 14.

School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 11031, Taiwan; Stem Cell Research Center, College of Oral Medicine, Taipei Medical University, Taipei 11031, Taiwan; Graduate Institute of Basic Medicine, Fu Jen Catholic University, Taipei, Taiwan; Department of Life Science, Tunghai University, Taichung, Taiwan. Electronic address:

Background: Cerebral ischemic events, comprising of excitotoxicity, reactive oxygen production, and inflammation, adversely impact the metabolic-redox circuit in highly active neuronal metabolic profile which maintains energy-dependent brain activities. Therefore, we investigated neuro-regenerative potential of melatonin (Mel), a natural biomaterial secreted by pineal gland.

Methods: We specifically determined whether Mel could influence tunneling nanotubes (TNTs)-mediated transfer of functional mitochondria (Mito) which in turn may alter membrane potential, oxidative stress and apoptotic factors. Read More

View Article and Full-Text PDF

Multifunctional nanoparticles as theranostic agents for therapy and imaging of breast cancer.

J Photochem Photobiol B 2020 Dec 29;218:112110. Epub 2020 Dec 29.

Institute for Biomedical Engineering, Science and Technology (iBEST), a partnership between Ryerson University and St. Michael's Hospital, Toronto, ON M5B 1T8, Canada; Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto, ON, M5B 1T8, Canada; Department of Physics, Ryerson University, Toronto, ON M5B 2K3, Canada. Electronic address:

Over the last decade, there has been significant developments in nanotechnology, in particular for combined imaging and therapeutic applications (theranostics). The core or shell of nanoemulsions (NEs) can be loaded with various therapeutic agents, including drugs with low solubility for effective treatment, or various imaging agents for specific imaging modalities (e.g. Read More

View Article and Full-Text PDF
December 2020

High levels of Tim-3Foxp3Treg cells in the tumor microenvironment is a prognostic indicator of poor survival of diffuse large B cell lymphoma patients.

Int Immunopharmacol 2021 Apr 14;96:107662. Epub 2021 Apr 14.

Department of Geriatrics, Gastroenterology Ward, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510180, China. Electronic address:

Foxp3Treg cells display phenotypic and functional heterogeneity, which express high levels of T cell immunoglobulin and mucin-domain containing-3 (Tim-3) in the tumor microenvironment (TME) of colorectal and lung cancer. High abundance of Tim-3Foxp3Treg (TFT) cells are associated with poor prognosis in these patients. However, the expression patterns and roles of TFT cells in TME of diffuse large B cell lymphoma (DLBCL) remain to be established. Read More

View Article and Full-Text PDF

Differences in growth of Trypanoplasma borreli in carp serum is dependent on transferrin genotype.

Fish Shellfish Immunol 2021 Apr 14. Epub 2021 Apr 14.

Polish Academy of Sciences, Institute of Ichthyobiology and Aquaculture in Gołysz, Zaborze, 43-520, Chybie, Poland. Electronic address:

Kinetoplastid parasites require transferrin (Tf), being the main source of iron, for growth and multiplication. This group of parasites developed a unique receptor-mediated system for acquiring host Tf which bears no structural homology with the host transferrin receptor. Trypanoplasma borreli, a blood parasite of common carp, probably uses a similar mechanism to sequester iron from host transferrin. Read More

View Article and Full-Text PDF

Comparative in vitro susceptibility of a novel fluoroquinolone antibiotic candidate WFQ-228, levofloxacin and moxifloxacin against Mycobacterium tuberculosis.

Int J Infect Dis 2021 Apr 14. Epub 2021 Apr 14.

Department of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University/Beijing Tuberculosis & Thoracic Tumor Research Institute, Beijing, 101149, PR China. Electronic address:

Objective: WFQ-228 is a novel developed fluoroquinolone (FQ) displaying potent antimicrobial activity against various clinical isolates of pathogens, including FQ-resistant isolates. The aim was to comparatively analyse in vitro susceptibilities of WFQ-228, levofloxacin (LFX) and moxifloxacin (MFX) against Mycobacterium tuberculosis (MTB) isolates, especially with gyrA mutations.

Methods: We selected a panel of 75 MTB isolates, consisting of 25 FQ-susceptible and 50 FQ-resistant isolates determined by conventional drug susceptibility testing. Read More

View Article and Full-Text PDF

Combinatorial lipid-nanosystem for dermal delivery of 5-fluorouracil and resveratrol against skin cancer: Delineation of improved dermatokinetics and epidermal drug deposition enhancement analysis.

Eur J Pharm Biopharm 2021 Apr 14. Epub 2021 Apr 14.

Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi -110062, INDIA. Electronic address:

In the present study, combinatorial nanostructured lipid carrier gel of 5-fluorouracil and resveratrol was formulated, optimized and characterized to enhance permeation in between epidermis and dermis layers of the skin to obtain a synergistic effect against skin cancer. After extensive trials, a newly modified emulsiosonication method was developed and additionally, for the first time, stability studies were done in the beginning to optimize formulation technique, which exhibited two major benefits simultaneously; first, it provided best-optimized technique for preparation of combinatorial lipid-nanosystem, and secondly, it also demonstrated a detailed report card of durability of formulations. In vitro release study showed a significantly improved, slow and prolonged release of drugs from the optimized lipid-nanosystem (***p<0. Read More

View Article and Full-Text PDF

A small molecule nanodrug consisting of pH-sensitive ortho ester-dasatinib conjugate for cancer therapy.

Eur J Pharm Biopharm 2021 Apr 14. Epub 2021 Apr 14.

Engineering Research Center for Biomedical Materials, School of Life Science, Anhui University, 111 Jiulong Road, Hefei, Anhui Province 230601, P. R. China. Electronic address:

The main objective of this paper is to develop a self-delivered prodrug system with nanoscale characteristics to enhance the efficacy of tumor therapy. The pH-sensitive prodrug was composed of ortho ester-linked dasatinib (DAS-OE), which was further self-assembled with or without doxorubicin (DOX) to obtain two carrier-free nanoparticles (DOX/DAS-OE NPs or DAS-OE NPs). The prodrug-based nanoparticles united the superiorities of small molecules and nano-assemblies together and displayed well-defined structure, uniform spherical shape, high drug loading ratio and on-demand drug release behavior. Read More

View Article and Full-Text PDF

The heteromeric complex formed by dopamine receptor D5 and CCR9 leads the gut-homing of CD4 T-cells upon inflammation.

Cell Mol Gastroenterol Hepatol 2021 Apr 14. Epub 2021 Apr 14.

Laboratorio de Neuroinmunología, Fundación Ciencia & Vida, Ñuñoa (7780272), Santiago, Chile; Facultad de Medicina y Ciencia, Universidad San Sebastián, Providencia (7510156), Santiago, Chile. Electronic address:

Background And Aims: CD4 T-cells constitute central players in inflammatory bowel diseases (IBD), driving inflammation in the gut-mucosa. Current evidence indicates that CCR9 and the integrin α4β7 are necessary and sufficient to imprint colonic homing on CD4 T-cells upon inflammation. Interestingly, dopaminergic signalling has been previously involved in leukocyte homing. Read More

View Article and Full-Text PDF

Scutellaria barbata D.Don and Oldenlandia diffusa (Willd.) Roxb crude extracts inhibit hepatitis-B-virus-associated hepatocellular carcinoma growth through regulating circRNA expression.

J Ethnopharmacol 2021 Apr 14:114110. Epub 2021 Apr 14.

Department of Pathology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Chinese Medicine, Shanghai, 200437, Shanghai, China. Electronic address:

Ethnopharmacological Relevance: Scutellaria barbata D.Don (SB) and Oldenlandia diffusa (Willd.) Roxb are commonly known as Ban Zhi Lian and Bai Hua She Cao in Chinese herbal medicines, respectively. Read More

View Article and Full-Text PDF

Zirconia-Reinforced Lithium Silicate (ZLS) mechanical and biological properties: a literature review.

J Dent 2021 Apr 14:103661. Epub 2021 Apr 14.

Department of Neurosciences, Reproductive and Odontostomatological Sciences, Division of Prosthodontics and Digital Dentistry, University "Federico II" of Naples, Via Pansini 5, 80131, Naples, Italy. Electronic address:

Objectives: This paper aimed to provide a literature review of the mechanical and biological properties of zirconia-reinforced lithium silicate glass-ceramics (ZLS) in Computer-aided design / Computer-aided manufacturing (CAD/CAM) systems.

Data/sources: An extensive search of the literature for papers related to ZLS was made on the databases of PubMed/Medline, Scopus, Embase, Google Scholar, Dynamed, and Open Grey. The papers were selected by 3 independent calibrated reviewers. Read More

View Article and Full-Text PDF

G protein gamma 7 suppresses progression of lung adenocarcinoma by inhibiting E2F transcription factor 1.

Int J Biol Macromol 2021 Apr 14. Epub 2021 Apr 14.

Department of radiation oncology, QiLu hospital of ShanDong university, Jinan, Shandong 250012, PR China. Electronic address:

G protein gamma 7 (GNG7) has been found to be aberrantly expressed in some kinds of malignant tumors. In this study, we mainly discuss the antitumor role of it in lung adenocarcinoma (LUAD) cells. Protein levels of GNG7 in LUAD tissues were measured by western blot and immunohistochemical analysis. Read More

View Article and Full-Text PDF

A distinct structure of Cas1-Cas2 complex provides insights into the mechanism for the longer spacer acquisition in Pyrococcus furiosus.

Int J Biol Macromol 2021 Apr 14. Epub 2021 Apr 14.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, PR China. Electronic address:

In the adaptation stage of CRISPR-Cas systems, the Cas1-Cas2 integrase captures and integrates new invader-derived spacers into the CRISPR locus, serving as a molecular memory of prior infection. As of yet, the structural information of Cas1-Cas2 complex is available only for two species. Here we present the crystal structure of Cas1-Cas2 complex of Pyrococcus furiosus, which showed a distinct architecture from the known Cas1-Cas2 complexes. Read More

View Article and Full-Text PDF

Carboxylesterases and arylacetamide deacetylase comparison in human A549, H460, and H727 pulmonary cells.

Life Sci 2021 Apr 14:119486. Epub 2021 Apr 14.

National Research Council (NRC), Institute of Agricultural Biology and Biotechnology (IBBA), Research Unit of Pisa, Via Moruzzi 1, 56124 Pisa, Italy.

Aims: Human carboxylesterases (CESs) and arylacetamide deacetylase (AADAC) are serine-esterase enzymes catalyzing the hydrolysis of many compounds containing esters, amides, thioesters, or acetyl groups. This study aimed to investigate the presence, kinetic parameters, and inhibition of CES1, CES2, and AADAC in A549, H460, and H727 pulmonary cells in both living cells and S9 fractions.

Materials And Methods: The p-nitrophenyl acetate (pNPA) and 4-methylumbelliferyl acetate (4-MUA) were used as non-selective esterase substrates, whereas phenacetin as selective AADAC substrate. Read More

View Article and Full-Text PDF

Anti-inflammatory effects of FS48, the first potassium channel inhibitor from the salivary glands of the flea Xenopsylla cheopis.

J Biol Chem 2021 Apr 14:100670. Epub 2021 Apr 14.

Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. Electronic address:

The voltage-gated potassium (Kv) 1.3 channel plays a crucial role in the immune responsiveness of T-lymphocytes and macrophages, presenting a potential target for treatment of immune- and inflammation related-diseases. FS48, a protein from the rodent flea Xenopsylla cheopis, shares the three disulfide bond feature of scorpion toxins. Read More

View Article and Full-Text PDF

SAGA and SAGA-like SLIK transcriptional coactivators are structurally and biochemically equivalent.

J Biol Chem 2021 Apr 14:100671. Epub 2021 Apr 14.

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia. Electronic address:

The SAGA-like complex SLIK is a modified version of the Spt-Ada-Gcn5-Acetyltransferase (SAGA) complex. SLIK is formed through C-terminal truncation of the Spt7 SAGA subunit, causing loss of Spt8, one of the subunits that interacts with the TATA-binding protein (TBP). SLIK and SAGA are both coactivators of RNA polymerase II transcription in yeast and both SAGA and SLIK perform chromatin modifications. Read More

View Article and Full-Text PDF

Inhibition of soluble epoxide hydrolase alleviates insulin resistance and hypertension via downregulation of SGLT2 in the mouse kidney.

J Biol Chem 2021 Apr 14:100667. Epub 2021 Apr 14.

Division of Cardiology and Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders, Wuhan 430030, China. Electronic address:

The epoxyeicosatrienoic acid (EET) exerts beneficial effects on insulin resistance and/or hypertension. EETs could be readily converted to less biological active diols by soluble epoxide hydrolase (sEH). However, whether sEH inhibition can ameliorate the comorbidities of insulin resistance and hypertension, and the underlying mechanisms of this relationship, are unclear. Read More

View Article and Full-Text PDF

α-PPP and its derivatives are selective partial releasers at the human norepinephrine transporter A pharmacological characterization of interactions between pyrrolidinopropiophenones and high and low affinity monoamine transporters.

Neuropharmacology 2021 Apr 14:108570. Epub 2021 Apr 14.

Medical University of Vienna, Center for Physiology and Pharmacology, Institute of Pharmacology, Währingerstraße 13A, 1090, Vienna, Austria; AddRess Centre for Addiction Research and Science, Medical University of Vienna, Währingerstraße 13A, 1090, Vienna, Austria. Electronic address:

While classical cathionones, such as methcathinone, have been shown to be monoamine releasing agents at human monoamine transporters, the subgroup of α-pyrrolidinophenones has thus far solely been characterized as monoamine transporter reuptake inhibitors. Herein, we report data from previously undescribed α-pyrrolidinopropiophenone (α-PPP) derivatives and compare them with the pharmacologically well-researched α-PVP (α-pyrrolidinovalerophenone). Radiotracer-based in vitro uptake inhibition assays in HEK293 cells show that the investigated α-PPP derivatives inhibit the human high-affinity transporters of dopamine (hDAT) and norepinephrine (hNET) in the low micromolar range, with α-PVP being ten times more potent. Read More

View Article and Full-Text PDF

Cardiomyopathy Mutations Impact the Actin-Activated Power Stroke of Human Cardiac Myosin.

Biophys J 2021 Apr 14. Epub 2021 Apr 14.

Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA 17033. Electronic address:

Cardiac muscle contraction is driven by the molecular motor myosin that uses the energy from ATP hydrolysis to generate a power stroke when interacting with actin filaments, while it is unclear how this mechanism is impaired by mutations in myosin that can lead to heart failure. We have applied a FRET strategy to investigate structural changes in the lever arm domain of human β-cardiac myosin subfragment 1 (M2β-S1). We exchanged the human ventricular regulatory light chain labeled at a single cysteine (V105C) with Alexa 488 onto M2β-S1, which served as a donor for Cy3ATP bound to the active site. Read More

View Article and Full-Text PDF

Subvisible Particles in Solutions of Remicade in Intravenous Saline Activate Immune System Pathways in In Vitro Human Cell Systems.

J Pharm Sci 2021 Apr 14. Epub 2021 Apr 14.

Department of Pharmaceutical Sciences, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, 80045. Electronic address:

Among patients that receive Remicade® therapy, more than 20% have adverse infusion related reactions and approximately 50% have immunogenic responses. Upon characterization of initial Remicade®-IV solution we observed a high concentration of subvisible particles that could inadvertently be delivered to patients. This solution was processed through the IV infusion system, mimicking the typical clinical administration setup - either with or without an in-line filter connected to the IV line. Read More

View Article and Full-Text PDF

Polymeric lipid hybrid nanoparticles as a delivery system enhance the antitumor effect of Emodin in vitro and in vivo.

J Pharm Sci 2021 Apr 14. Epub 2021 Apr 14.

College of Pharmacy, Jinan University, Guangzhou, 510632, China; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE), School of Pharmacy, Jinan University, Guangzhou, 510632, China; Cancer Research Institute of Jinan University, Guangzhou, 510632, China; Interventional Cancer Institute of Chinese Integrative Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China. Electronic address:

This study aimed to evaluate the therapeutic efficacy of Emodin-loaded polymer lipid hybrid nanoparticles (E-PLNs) for breast cancer. The size, Zeta potential, surface morphology, encapsulation efficiency, stability, in vitro drug release of E-PLNs prepared by the nanoprecipitation method were characterized. The uptake, in-vitro cytotoxicities and apoptosis of free drug, E-PLNs were investigated against MCF-7 cells. Read More

View Article and Full-Text PDF

In vitro Antifungal susceptibility patterns of Trichophyton benhamiae complex isolates from diverse origin.

Mycoses 2021 Apr 17. Epub 2021 Apr 17.

Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Background: Species from the Trichophyton benhamiae complex are mostly zoophilic dermatophytes which cause inflammatory dermatophytosis in animals and humans worldwide.

Objectives: This study was purposed to 1) to identify 169 reference and clinical dermatophyte strains from the T. benhamiae complex species by molecular method and adhering to the newest taxonomy in the complex 2) to evaluate the in vitro antifungal susceptibility profile of these strains against eight common and new antifungal agents that may be used for the treatment of dermatophytosis. Read More

View Article and Full-Text PDF