Neuropathol Appl Neurobiol 2020 12 1;46(7):751-769. Epub 2020 May 1.
Ilsong Institute of Life Science, Hallym University, Anyang, Gyeonggi-do, Republic of Korea.
Aims: Vimentin citrullination, the calcium (Ca )-dependent peptidylarginine deiminase (PAD)-mediated conversion of an arginine residue of vimentin to a citrulline residue, has emerged as a pathophysiological outcome of autoimmune diseases and neurodegeneration. However, the roles, functions, and expression of citrullinated vimentin have not yet been elucidated because available antibodies are limited.
Methods: We developed mouse monoclonal IgG1 and IgM specific for vimentin citrullinated at position R440 or R450 and applied Western blotting, immunohistochemistry, and immunofluorescent staining to investigate the pathogenesis of prion diseases in animal models, in patients with prion diseases, and in vitro. Read More