7,282 results match your criteria viability enhance


Baicalein modulates the radiosensitivity of cervical cancer cells in vitro via miR-183 and the JAK2/STAT3 signaling pathway.

Adv Clin Exp Med 2021 Jun 11. Epub 2021 Jun 11.

Department of Radiation Oncology, The Second Hospital of Dalian Medical University, China.

Background: Increasing radiosensitivity of cancer cells can enhance the efficacy of cervical cancer treatment.

Objectives: This study evaluated the potential roles and mechanism of baicalein in regulating the radiosensitivity of cervical cancer cells in vitro.

Material And Methods: Real-time quantitative polymerase chain reaction (RT-qPCR) was used to assess miR-183 expression in End1/E6E7 cells, Hela cells and Hela cells irradiated with X-ray (0 Gy, 1 Gy, 3 Gy, 5 Gy, and 10 Gy). Read More

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Progress in cardiac research - from rebooting cardiac regeneration to a complete cell atlas of the heart.

Cardiovasc Res 2021 Jun 11. Epub 2021 Jun 11.

Institute for Cardiovascular Prevention (IPEK), LMU Munich, DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance, and Munich Cluster for Systems Neurology (SyNergy), Munich, Germany.

We review some of the important discoveries and advances made in basic and translational cardiac research in 2020. For example, in the field of myocardial infarction (MI), new aspects of autophagy and the importance of eosinophils were described. Novel approaches such as a glycocalyx mimetic were used to improve cardiac recovery following MI. Read More

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A chondrosphere-based scaffold free approach to manufacture an in-vitro articular cartilage model.

Tissue Eng Part A 2021 Jun 11. Epub 2021 Jun 11.

Newcastle University, 5994, School of Engineering, Claremont Road, Newcastle upon Tyne, Tyne and Wear, United Kingdom of Great Britain and Northern Ireland, NE1 7RU;

In-vitro engineering of human articular cartilage (AC) is a regenerative medicine challenge. The main objective of this study was the development of a repeatable scaffold-free in-vitro model of chondrocyte spheroid-based treatments of cartilage defects, to allow for systematic study and further optimisation of this type of treatment. Human articular chondrocytes (HC) and immortalised mesenchymal cells differentiated in chondrocytes (Y201-Cs) were cultured in round-bottom 96-well plates to produce multicellular spheroids and their growth kinetics and viability was evaluated over 7 days of culture. Read More

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Biotin-streptavidin-guided two-step pretargeting approach using PLGA for molecular ultrasound imaging and chemotherapy for ovarian cancer.

PeerJ 2021 25;9:e11486. Epub 2021 May 25.

Ultrasound Medicine Department, Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, Shapingba District, China.

Background: Ovarian cancer seriously threatens the lives and health of women, and early diagnosis and treatment are still challenging. Pre-targeting is a promising strategy to improve the treatment efficacy of ovarian cancer and the results of ultrasound imaging.

Purpose: To explore the effects of a pre-targeting strategy using streptavidin (SA) and paclitaxel (PTX)-loaded phase-shifting poly lactic-co-glycolic acid (PLGA) nanoparticles with perfluoro-n-pentane (PTX-PLGA-SA/PFPs) on the treatment and ultrasound imaging of ovarian cancer. Read More

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circMTO1 sponges microRNA-219a-5p to enhance gallbladder cancer progression via the TGF-β/Smad and EGFR pathways.

Oncol Lett 2021 Jul 27;22(1):563. Epub 2021 May 27.

Department of Hepatobiliary Surgery, Lanzhou No. 2 People's Hospital, Lanzhou, Gansu 730046, P.R. China.

Circular mitochondrial translation optimization 1 homologue (circMTO1) has been reported to regulate the tumorigenesis of different types of cancer; however, the role of circMTO1 in gallbladder cancer (GBC) remains unknown. The present study aimed to identify the potential miRNAs and target genes of circMTO1 during GBC progression, and clarify the regulatory mechanism between circMTO1 and miRNAs or target genes. The present study performed MTT and Transwell assays, and Annexin V staining to assess cell viability, migration and apoptosis, respectively. Read More

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β-Elemene Enhances the Sensitivity of Osteosarcoma Cells to Doxorubicin via Downregulation of Peroxiredoxin-1.

Onco Targets Ther 2021 3;14:3599-3609. Epub 2021 Jun 3.

Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, People's Republic of China.

Background: Doxorubicin (Dox) resistance is a primary obstacle for the treatment of osteosarcoma. Meanwhile, β-Elemene was shown to exhibit an anti-proliferative effect on osteosarcoma cells. However, the role of a combination of Dox with β-Elemene on osteosarcoma cells remains unclear. Read More

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Antimicrobial Peptide-Loaded Pectolite Nanorods for Enhancing Wound-Healing and Biocidal Activity of Titanium.

ACS Appl Mater Interfaces 2021 Jun 10. Epub 2021 Jun 10.

Department of Chemistry, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States.

Titanium is widely utilized for manufacturing medical implants due to its inherent mechanical strength and biocompatibility. Recent studies have focused on developing coatings to impart unique properties to Ti implants, such as antimicrobial behavior, enhanced cell adhesion, and osteointegration. Ca- and Si-based ceramic (CS) coatings can enhance bone integration through the release of Ca and Si ions. Read More

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T cells, particularly activated CD4 cells, maintain anti-CD20-mediated NK cell viability and antibody dependent cellular cytotoxicity.

Cancer Immunol Immunother 2021 Jun 10. Epub 2021 Jun 10.

Cancer Biology Graduate Program, Holden Comprehensive Cancer Center, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA.

Anti-CD20 monoclonal antibody (mAb) therapy is a mainstay of therapy for B cell malignancies, however many patients fail to respond or eventually develop resistance. The current understanding of mechanisms responsible for this resistance is limited. When peripheral blood mononuclear cells of healthy donors were cultured with Raji cells for 7 days, rituximab (RTX) induced NK cell-mediated antibody-dependent cellular cytotoxicity (ADCC), enhanced NK cell viability and increased or maintained NK expression of CD56, CD16, CD57 and KIR. Read More

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The tobacco phosphatidylethanolamine-binding protein NtFT4 simultaneously improves vitality, growth and protein yield in human cells.

Biotechnol Bioeng 2021 Jun 10. Epub 2021 Jun 10.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Münster, Germany.

The production of biopharmaceutical proteins in mammalian cells by transient expression or stable transformation requires robust and viable cells. Cell line engineering must therefore balance improved cell growth and viability with high productivity. We tested the ability of non-mammalian phosphatidylethanolamine-binding proteins to enhance cell proliferation in monolayers and suspension cultures. Read More

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Dual Functions of Riboflavin-functionalized Poly(lactic-co-glycolic acid) Nanoparticles for Enhanced Drug Delivery Efficiency and Photodynamic Therapy in Triple-negative Breast Cancer Cells.

Photochem Photobiol 2021 Jun 10. Epub 2021 Jun 10.

School of Materials Science and Innovation, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, 10400, Thailand.

Combating triple-negative breast cancer (TNBC) is one of the greatest challenges in cancer therapy. This is primarily due to the difficulties in developing drug delivery systems that can effectively target cancer sites. In this study, we demonstrated a proof-of-principle concept using modified surfaces of poly(lactic-co-glycolic acid) nanoparticles linked with a riboflavin analogue (PLGA-CSRf) to obtain a dual functional material. Read More

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Micronucleus Formation Induced by Glyphosate and Glyphosate-Based Herbicides in Human Peripheral White Blood Cells.

Front Public Health 2021 24;9:639143. Epub 2021 May 24.

Department of Public Health and Epidemiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Glyphosate is the most commonly used herbicide around the world, which led to its accumulation in the environment and consequent ubiquitous human exposure. Glyphosate is marketed in numerous glyphosate-based herbicide formulations (GBHs) that include co-formulants to enhance herbicidal effect of the active ingredient, but are declared as inert substances. However, these other ingredients can have biologic activity on their own and may interact with the glyphosate in synergistic toxicity. Read More

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CHOP Regulates Endoplasmic Reticulum Stress-Mediated Hepatoxicity Induced by Monocrotaline.

Front Pharmacol 2021 24;12:685895. Epub 2021 May 24.

College of Veterinary Medicine, Northwest A&F University, Yangling, China.

Monocrotaline (MCT), a pyrrolizidine alkaloid, is the major toxin in , which causes cell apoptosis in humans and animals. It has been reported that the liver is a vulnerable target of MCT. However, the exact molecular mechanism of the interaction between endoplasmic reticulum (ER) stress and liver injury induced by MCT is still unclear. Read More

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Systematic screening reveals synergistic interactions that overcome MAPK inhibitor resistance in cancer cells.

Cancer Biol Med 2021 Jun 9. Epub 2021 Jun 9.

Department of Cell Biology, Basic Medical College, Army Medical University (Third Military Medical University), Chongqing 400038, China.

Objective: Effective adjuvant therapeutic strategies are urgently needed to overcome MAPK inhibitor (MAPKi) resistance, which is one of the most common forms of resistance that has emerged in many types of cancers. Here, we aimed to systematically identify the genetic interactions underlying MAPKi resistance, and to further investigate the mechanisms that produce the genetic interactions that generate synergistic MAPKi resistance.

Methods: We conducted a comprehensive pair-wise sgRNA-based high-throughput screening assay to identify synergistic interactions that sensitized cancer cells to MAPKi, and validated 3 genetic combinations through competitive growth, cell viability, and spheroid formation assays. Read More

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Effect of iron addition on mAb productivity and oxidative stress in CHO culture.

Biotechnol Prog 2021 Jun 9. Epub 2021 Jun 9.

Dept. of Chemical Engineering, University of Massachusetts Lowell, Lowell, MA.

Trace metals play a critical role in the development of media used for the production of therapeutic proteins. Iron has been shown to enhance the productivity of monoclonal antibodies (mAbs) during Chinese hamster ovary (CHO) cell culture. However, the redox activity and pro-oxidant behavior of iron may also contribute towards the production of reactive oxygen species (ROS). Read More

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A review of animal models from 2015 to 2020 for preclinical chronic wounds relevant to human health.

J Tissue Viability 2021 Jun 1. Epub 2021 Jun 1.

Louis Stokes Cleveland Veterans Affairs Medical Center (LSCVAMC), Cleveland, OH, USA; Dept of Orthopaedics & Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA. Electronic address:

Significance: Chronic wounds fail to heal in a timely manner and exhibit sustained inflammation with slow tissue repair and remodelling. They decrease mobility and quality of life, and remain a major clinical challenge in the long-term care of many patients, affecting 6.5 million individuals annually in the U. Read More

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Evaluation of Silibinin-Loaded Microbubbles Combined with Ultrasound in Ovarian Cancer Cells: Cytotoxicity and Mechanisms.

Anticancer Agents Med Chem 2021 Jun 7. Epub 2021 Jun 7.

Department of Thyroid Surgery, Qilu Hospital of Shandong University, Jinan, China.

Background: The anticancer activity of silibinin (SB) has been demonstrated in various cancer cell types. However, its low solubility and poor bioavailability limit its clinical potential in biomedical applications. Microbubbles in combination with ultrasound are promising vehicles for local drug delivery. Read More

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Protective Role of PERK-eIF2α-ATF4 Pathway in Chronic Renal Failure Induced Injury of Rat Hippocampal Neurons.

Int J Neurosci 2021 Jun 8:1-13. Epub 2021 Jun 8.

Department of Nephrology, The First People's Hospital of Huzhou, Huzhou, Zhejiang, China.

Chronic renal failure (CRF) is associated with impairment of hippocampal neurons. This study investigated the effect of PERK-eIF2α-ATF4 pathway in CRF. Rat CRF model was established and rat hippocampal neurons were separated. Read More

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Oxidant activated soluble adenylate cyclase of Leishmania donovani regulates the cAMP-PKA signaling axis for its intra-macrophage survival during infection.

J Cell Biochem 2021 Jun 8. Epub 2021 Jun 8.

Department of Molecular Biology, ICMR-Rajendra Memorial Research Institute of Medical Sciences, Agamkuan, Patna, Bihar, India.

Adenosine 3',5'-cyclic monophosphate (cAMP) is a stress sensor molecule that transduces the cellular signal when Leishmania donovani moves from insect vector to mammalian host. At this stage, the parasite membrane-bound receptor adenylate cyclase predominantly produces cAMP to cope with the oxidative assault imposed by host macrophages. However, the role of soluble adenylate cyclase of L. Read More

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Three-Dimensionally Printed Skin Substitute Using Human Dermal Fibroblasts and Human Epidermal Keratinocytes.

Ann Plast Surg 2021 Jun;86(6S Suppl 5):S628-S631

Department of Biomedical Engineering.

Introduction: Wound healing affects millions of people annually. After injury, keratinocytes from the wound edge proliferate, migrate, and differentiate to recapitulate the 3-dimensional (3D) structure needed to provide a barrier function. If the wound is too large, skin grafting may be required. Read More

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Observing quantum coherence from photons scattered in free-space.

Light Sci Appl 2021 Jun 7;10(1):121. Epub 2021 Jun 7.

Institute for Quantum Computing, University of Waterloo, Waterloo, ON, N2L 3G1, Canada.

Quantum channels in free-space, an essential prerequisite for fundamental tests of quantum mechanics and quantum technologies in open space, have so far been based on direct line-of-sight because the predominant approaches for photon-encoding, including polarization and spatial modes, are not compatible with randomly scattered photons. Here we demonstrate a novel approach to transfer and recover quantum coherence from scattered, non-line-of-sight photons analyzed in a multimode and imaging interferometer for time-bins, combined with photon detection based on a 8 × 8 single-photon-detector-array. The observed time-bin visibility for scattered photons remained at a high 95% over a wide scattering angle range of -45 to +45, while the individual pixels in the detector array resolve or track an image in its field of view of ca. Read More

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Characterization of a commercially available line of iPSC hepatocytes as models of hepatocyte function and toxicity for regulatory purposes.

J Pharmacol Toxicol Methods 2021 Jun 4:107083. Epub 2021 Jun 4.

United States Food and Drug Administration, Center for Drug Evaluation and Research, Office of Translational Sciences, Office of Clinical Pharmacology, Division of Applied Regulatory Science, 10903 New Hampshire Ave, Silver Spring, MD 20993-0002, United States of America. Electronic address:

It has recently become possible to produce hepatocytes from human induced pluripotent stem cells (iPSC-heps), which may offer some advantages over primary human hepatocytes (Prim-heps) in the regulatory environment. The aim of this research was to assess similarities and differences between commercially available iPSC-heps and Prim-heps in preliminary assays of drug metabolism, hepatotoxicity, and drug transport. Hepatocytes were either cultured in collagen-coated 96-well plates (Prim-heps and 2d-iPSC-heps) or in ultra-low adhesion plates as spheroids (3d-iPSC-heps). Read More

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Curcumin loaded chitosan-protamine nanoparticles revealed antitumor activity via suppression of NF-κB, proinflammatory cytokines and Bcl-2 gene expression in the breast cancer cells.

J Pharm Sci 2021 Jun 4. Epub 2021 Jun 4.

Department of Pharmaceutical Biotechnology, College of Biotechnology, Misr University for Science and Technology P. O. Box 77, Giza, Egypt.

Nano drug delivery has been recently used to enhance the stability and bioavailability of chemotherapeutic agents. In this study, Chitosan/protamine nanocarrier was synthesized and used to encapsulate curcumin (CUR). The physicochemical properties of the empty carrier (CHPNPs) and curcumin-containing carrier (CU-CHPNPs) were characterized by TEM imaging, Zetasizer, and FT-IR spectroscopy. Read More

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Atg32-dependent mitophagy sustains spermidine and nitric oxide required for heat-stress tolerance in Saccharomycescerevisiae.

J Cell Sci 2021 Jun 7;134(11). Epub 2021 Jun 7.

Department of Pathology and Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, California 94143, USA.

In Saccharomyces cerevisiae, the selective autophagic degradation of mitochondria, termed mitophagy, is critically regulated by the adapter protein Atg32. Despite our knowledge about the molecular mechanisms by which Atg32 controls mitophagy, its physiological roles in yeast survival and fitness remains less clear. Here, we demonstrate a requirement for Atg32 in promoting spermidine production during respiratory growth and heat-induced mitochondrial stress. Read More

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Substantial cell apoptosis provoked by naked PAMAM dendrimers in HER2-positive human breast cancer via JNK and ERK1/ERK2 signalling pathways.

Comput Struct Biotechnol J 2021 7;19:2881-2890. Epub 2021 May 7.

College of Pharmacy, QU Health, Qatar University, PO Box 2713, Doha, Qatar.

HER2-positive breast cancer is one of its most challenging subtypes, forming around 15-25% of the total cases. It is characterized by aggressive behavior and treatment resistance. On the other hand, poly (amidoamine) (PAMAM) dendrimers are widely used in drug delivery systems and gene transfection as carriers. Read More

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Enhanced Reproductive Thermotolerance of the Tomato Mutant Is Associated With Increased Accumulation of Flavonols in Pollen.

Front Plant Sci 2021 19;12:672368. Epub 2021 May 19.

Institute of Plant Sciences, Agricultural Research Organization - Volcani Center, Rishon LeZion, Israel.

Climate change has created an environment where heat stress conditions are becoming more frequent as temperatures continue to raise in crop production areas around the world. This situation leads to decreased crop production due to plant sensitivity to heat stress. Reproductive success is critically dependent on plants' ability to produce functional pollen grains, which are the most thermo-sensitive tissue. Read More

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Formulation of inherently antimicrobial magnesium oxychloride cement and the effect of supplementation with silver phosphate.

Mater Sci Eng C Mater Biol Appl 2021 Jul 30;126:112158. Epub 2021 Apr 30.

School of Chemical Engineering, University of Birmingham, Edgbaston B15 2TT, United Kingdom. Electronic address:

The growing threat of bacterial resistance to antibiotics is driving an increasing need for new antimicrobial strategies. This work demonstrates the potential of magnesium oxychloride cements (MOC) to be used as inorganic antimicrobial biomaterials for bone augmentation. An injectable formulation was identified at a powder to liquid ratio of 1. Read More

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Hsa_circ_0001982 promotes the progression of breast cancer through miR-1287-5p/MUC19 axis under hypoxia.

World J Surg Oncol 2021 Jun 3;19(1):161. Epub 2021 Jun 3.

Department of Oncology, Huaian Second People's Hospital, The Affiliated Huaian Hospital of Xuzhou Medical University, No. 62 Huaihai South Road, Huai'an City, Jiangsu, China.

Background: Breast cancer (BC) is the most commonly malignant tumor among women worldwide. Many studies have reported that circular RNAs (circRNAs) were participated in the regulation of multiple cancers development. However, the mechanism underlying hsa_circ_0001982 in breast cancer development is still unclear. Read More

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Angiopoietin-like protein 4 regulates breast muscle lipid metabolism in broilers.

Poult Sci 2021 Mar 26;100(7):101159. Epub 2021 Mar 26.

College of Animal Sciences and Technology, Shandong Agricultural University, Tai-an, Shandong, 271018, P. R. China.

The objective of this study was to determine the effects of angiopoietin-like protein 4 (ANGPTL4) on breast muscle lipid metabolism in broilers. In experiment 1, 36 thirty-five-day-old male Arbor Acres broilers were randomly allocated into 6 treatment groups with 6 birds in a completely randomized design. The broilers were subjected to intravenous injection of His-SUMO-ANGPTL4 at the dose of 0 (injection of normal saline [NS]), 20, 100, 500, 2,500, or 12,500 ng/kg BW, respectively. Read More

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Circ_0091702 relieves lipopolysaccharide (LPS)-induced cell injury by regulating the miR-182/PDE7A axis in sepsis.

Biosci Biotechnol Biochem 2021 Jun 2. Epub 2021 Jun 2.

Departmentof Emergency, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051, Hebei, China.

Circular RNA (circRNA) plays an important role in the progression of sepsis. Circ_0091702 has been found to be an important regulator of sepsis progression, so its role and mechanism in sepsis progression deserve to be further explored. LPS could suppress cell viability, while enhance cell apoptosis and inflammation to induce cell injury. Read More

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Role of ZEB family members in proliferation, metastasis and chemoresistance of prostate cancer cells: Revealing signaling networks.

Curr Cancer Drug Targets 2021 Jun 1. Epub 2021 Jun 1.

Department of Pharmacy, Abdul Wali Khan University, Mardan, 23200. Pakistan.

Prostate cancer (PCa) is one of the leading causes of death worldwide. A variety of strategies including surgery, chemotherapy, radiotherapy and immunotherapy are applied for PCa treatment. PCa cells are responsive towards therapy at early stages, but they can obtain resistance in the advanced stage. Read More

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