5,876 results match your criteria vectors training


Red fluorescent AIEgens based multifunctional nonviral gene vectors for the efficient combination of gene therapy and photodynamic therapy in anti-cancer.

Colloids Surf B Biointerfaces 2022 Aug 8;218:112765. Epub 2022 Aug 8.

China National Institute for Food and Drug Control, Institute of Chemical Drug Control, HuaTuo Road 29, Beijing 102629, PR China. Electronic address:

Precise molecular engineering of AIEgens-based cationic delivery systems for high transfection efficiency (TE) and effective photodynamic therapy (PDT) holds a huge potential for cancer treatment. Herein, three amphiphiles (DT-C-M) consisting of di(triazole-[12]aneN) (M) and 1,1-dicyano-2-phenyl-2-(4-diphenylamino)phenyl-ethylene (DT) units have been developed to achieve luminescent tracking, efficient TE, and effective PDT in vitro and in vivo. These compounds exhibited strong aggregated induced emission (AIE) at 630 nm and mega Stokes shifts of up to 160 nm. Read More

View Article and Full-Text PDF

A Universal Framework for Featurization of Atomistic Systems.

J Phys Chem Lett 2022 Aug 18:7911-7919. Epub 2022 Aug 18.

School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.

Machine-learning force fields have become increasingly popular because of their balance of accuracy and speed. However, a significant limitation is the use of element-specific features, leading to poor scalability with the number of elements. This work introduces the Gaussian multipole (GMP) featurization scheme that utilizes physically relevant multipole expansions of the electron density around atoms to yield feature vectors that interpolate between element types and have a fixed dimension regardless of the number of elements present. Read More

View Article and Full-Text PDF

SARS-CoV-2 Transmission by Arthropod Vectors: A Scoping Review.

Biomed Res Int 2022 8;2022:4329423. Epub 2022 Aug 8.

Functional Neurosurgery Research Center, Shohada-e Tajrish Neurosurgical Center of Excellence, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

COVID-19 is a respiratory disease of worldwide importance as it has brought enormous health problems to the world's population. The best-known way of transmission of the virus is through aerosolization. However, research is needed to explore other transmission routes. Read More

View Article and Full-Text PDF

First Report of Hooper, 1961 (Nematoda: Longidoridae) from Greece.

J Nematol 2022 Feb 29;54(1):20220027. Epub 2022 Jul 29.

Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Centro Alameda del Obispo, 14004, Córdoba, Spain.

Longidorid nematodes comprise more than 500 species, and and are the most diversified, prevalent, and cosmopolitan genera within plant-parasitic nematodes. The genus comprise a group of species, some of which are vectors of plant viruses. New sampling for needle nematodes was carried out in a grapevine area in Thessaloniki, northern Greece, and one nematode species of () was recovered. Read More

View Article and Full-Text PDF
February 2022

Fine-scale-mapping of Schistosoma haematobium infections at the school and community levels and intermediate host snail abundance in the north of Pemba Island: baseline cross-sectional survey findings before the onset of a 3-year intervention study.

Parasit Vectors 2022 Aug 16;15(1):292. Epub 2022 Aug 16.

Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Allschwil, Switzerland.

Background: Schistosomiasis elimination has gained renewed priority in the WHO guidance documents published in 2020 and 2022. The SchistoBreak project, implemented in Pemba, Tanzania between 2020 and 2024, aims to assess new tools and strategies for shifting from elimination as a public health problem towards interruption of transmission. Here we report our baseline findings and discuss implications for future interventions. Read More

View Article and Full-Text PDF

Zoonotic infection caused by Onchocerca japonica (Nematoda: Filarioidea) in a 69-year-old woman in Kanto Region, Eastern Honshu, Japan.

Parasitol Int 2022 Aug 9;91:102643. Epub 2022 Aug 9.

Tropical Infectious Diseases Research and Education Centre, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.

Reports of zoonotic infections caused by the filarial nematode Onchocerca japonica have recently increased in Japan. A 69-year-old woman living in Sosa City, Chiba Prefecture, Kanto Region, Honshu, developed a painful nodule at the metacarpophalangeal joint of the index finger of her right hand. The causative agent was identified as a female O. Read More

View Article and Full-Text PDF

The IFN-γ-related long non-coding RNA signature predicts prognosis and indicates immune microenvironment infiltration in uterine corpus endometrial carcinoma.

Front Oncol 2022 26;12:955979. Epub 2022 Jul 26.

Department of Obstetrics and Gynecology, Nantong Haimen People's Hospital, Nantong, China.

Background: One of the most common diseases that have a negative impact on women's health is endometrial carcinoma (EC). Advanced endometrial cancer has a dismal prognosis and lacks solid prognostic indicators. IFN-γ is a key cytokine in the inflammatory response, and it has also been suggested that it has a role in the tumor microenvironment. Read More

View Article and Full-Text PDF

Edge-to-Cloud IIoT for Condition Monitoring in Manufacturing Systems with Ubiquitous Smart Sensors.

Sensors (Basel) 2022 Aug 7;22(15). Epub 2022 Aug 7.

City University of Hong Kong Shenzhen Research Institute, Shenzhen 518057, China.

The Industrial Internet of Things (IIoT) connects industrial assets to ubiquitous smart sensors and actuators to enhance manufacturing and industrial processes. Data-driven condition monitoring is an essential technology for intelligent manufacturing systems to identify anomalies from malfunctioning equipment, prevent unplanned downtime, and reduce the operation costs by predictive maintenance without interrupting normal machine operations. However, data-driven condition monitoring requires massive data collected from smart sensors to be transmitted to the cloud for further processing, thereby contributing to network congestion and affecting the network performance. Read More

View Article and Full-Text PDF

Spotted fever group Rickettsia, Anaplasma and Coxiella-like endosymbiont in Haemaphysalis ticks from mammals in Thailand.

Vet Res Commun 2022 Aug 9. Epub 2022 Aug 9.

Biodiversity Research Cluster, Department of Biology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.

Ticks are ectoparasites of vertebrates and vectors of various pathogenic microorganisms. In this study, the presence of bacteria and protozoa was evaluated by PCR and DNA sequencing in 233 mammal ticks collected from 8 provinces in Thailand. Sequence and phylogenetic analyses of partial rickettsial ompA, ompB, sca4 and partial Coxiella 16S rRNA, GroEL, rpoB genes clearly revealed, for the first time, a co-infection of SFG Rickettsia belonging to R. Read More

View Article and Full-Text PDF

Histidine acid phosphatase domain-containing protein from Haemonchus contortus is a stimulatory antigen for the Th1 immune response of goat PBMCs.

Parasit Vectors 2022 Aug 6;15(1):282. Epub 2022 Aug 6.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China.

Background: Histidine acid phosphatase (HAP), a member of the histidine phosphatase superfamily, is widely found in parasites and is also a potential vaccine antigen or drug target. However, the biological function of HAP in Haemonchus contortus is still unclear.

Methods: We cloned the HAP gene from H. Read More

View Article and Full-Text PDF

Effects of sample preservation methods and duration of storage on the performance of mid-infrared spectroscopy for predicting the age of malaria vectors.

Parasit Vectors 2022 Aug 6;15(1):281. Epub 2022 Aug 6.

Environmental Health and Ecological Science Department, Ifakara Health Institute, P.O. Box 53, Ifakara, Tanzania.

Background: Monitoring the biological attributes of mosquitoes is critical for understanding pathogen transmission and estimating the impacts of vector control interventions on the survival of vector species. Infrared spectroscopy and machine learning techniques are increasingly being tested for this purpose and have been proven to accurately predict the age, species, blood-meal sources, and pathogen infections in Anopheles and Aedes mosquitoes. However, as these techniques are still in early-stage implementation, there are no standardized procedures for handling samples prior to the infrared scanning. Read More

View Article and Full-Text PDF

RNA sequencing reveals dynamic expression of spleen lncRNAs and mRNAs in Beagle dogs infected by Toxocara canis.

Parasit Vectors 2022 Aug 4;15(1):279. Epub 2022 Aug 4.

School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.

Background: Toxocara canis is a cosmopolitan parasite with a significant adverse impact on the health of humans and animals. The spleen is a major immune organ that plays essential roles in protecting the host against various infections. However, its role in T. Read More

View Article and Full-Text PDF

Sub-national tailoring of seasonal malaria chemoprevention in Mali based on malaria surveillance and rainfall data.

Parasit Vectors 2022 Aug 4;15(1):278. Epub 2022 Aug 4.

Malaria Research and Training Centre Ogobara K. Doumbo (MRTC-OKD), FMOS-FAPH, Mali-NIAID-ICER, Université Des Sciences, Des Techniques Et Des Technologies de Bamako, 1805, Bamako, Mali.

Background: In malaria endemic countries, seasonal malaria chemoprevention (SMC) interventions are performed during the high malaria transmission in accordance with epidemiological surveillance data. In this study we propose a predictive approach for tailoring the timing and number of cycles of SMC in all health districts of Mali based on sub-national epidemiological surveillance and rainfall data. Our primary objective was to select the best of two approaches for predicting the onset of the high transmission season at the operational scale. Read More

View Article and Full-Text PDF

AAV-Mediated Gene Replacement Therapy Restores Viability of BCD Patient iPSC Derived RPE Cells and Vision of Cyp4v3 Knockout Mice.

Hum Mol Genet 2022 Aug 4. Epub 2022 Aug 4.

Key Laboratory of Vision Loss and Restoration, Department of Ophthalmology, Ministry of Education, Peking University Third Hospital, Beijing, People's Republic of China.

Bietti crystalline corneoretinal dystrophy (BCD) is an autosomal recessive retinal degenerative disease characterized by yellow-white crystal deposits in the posterior pole, degeneration of the retinal pigment epithelium (RPE), and sclerosis of the choroid. Mutations in the cytochrome P450 4 V2 gene (CYP4V2) cause BCD, which is associated with lipid metabolic disruption. The use of gene replacement therapy in BCD has been hampered by the lack of disease models. Read More

View Article and Full-Text PDF

Prototype mouse models for researching SEND-based mRNA delivery and gene therapy.

Nat Protoc 2022 Aug 3. Epub 2022 Aug 3.

Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Japan.

One of the major challenges of gene therapy-an approach to treat diseases caused by faulty genes-is a lack of technologies that deliver healthy gene copies to target tissues and cells. Some commonly used approaches include viral vectors or coating therapeutic nucleic acids with lipid-based nanoparticles to pass through cell membranes, but these technologies have had limited success. A revolutionary tool, the CRISPR-Cas gene-editing system, offers tremendous promise, but it too suffers from problems with delivery. Read More

View Article and Full-Text PDF

First report of haemosporidia and associated risk factors in red junglefowl (Gallus gallus) in China.

Parasit Vectors 2022 Aug 1;15(1):275. Epub 2022 Aug 1.

Animal Research and Resource Center, Yunnan University, Kunming, Yunnan Province, 650500, People's Republic of China.

Background: Avian haemosporidia infect both domestic and wild birds, causing anemia, acute tissue degeneration, and depopulation in wild birds. Poultry and wild birds have been reported as common reservoirs of haemosporidia, but limited information is available for red junglefowl (Gallus gallus) in China. The present study investigated the prevalence and molecular characterization of haemosporidia in red junglefowl. Read More

View Article and Full-Text PDF

Midguts of Culex pipiens L. (Diptera: Culicidae) as a potential source of raw milk contamination with pathogens.

Sci Rep 2022 Aug 1;12(1):13183. Epub 2022 Aug 1.

Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Cairo, 11241, Egypt.

Despite their importance, only few studies focused on the mosquitoes borne microbial diseases, especially bacterial and fungal diseases, their vectorial capacity toward microorganisms, and their important role in raw milk contamination with pathogens in some unsanitary dairy farms. In Egypt, where Culex pipiens is the historical main vector of lymphatic filariasis, only few studies discussed the isolation of pathogens from the midguts of different stages of C. pipiens. Read More

View Article and Full-Text PDF

Prevalence of Japanese encephalitis (JE) virus in mosquitoes and animals of the Asian continent: A systematic review and meta-analysis.

J Infect Public Health 2022 Jul 25;15(9):942-949. Epub 2022 Jul 25.

ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Bengaluru, Karnataka, India. Electronic address:

Background: Japanese encephalitis (JE) is a viral zoonotic disease that has been found in several countries of Asia and is responsible for high mortality and morbidity of men and animals in rural and sub-urban endemic areas due to the virus re-circulation among diverse hosts and vectors. The present study estimates the prevalence of the JE virus in the vector and animal population of the Asian continent using a systematic review and meta-analysis.

Methods: The Cochran collaborators' Preferred Reporting Items for Systematic Reviews and Meta-Analysis [PRISMA] guidelines were used for systematic review and meta-analysis. Read More

View Article and Full-Text PDF

Potential Gut Microbiota Features for Non-Invasive Detection of Schistosomiasis.

Front Immunol 2022 14;13:941530. Epub 2022 Jul 14.

Key Laboratory of Tropical Disease Control, Ministry of Education, Guangzhou, China.

The gut microbiota has been identified as a predictive biomarker for various diseases. However, few studies focused on the diagnostic accuracy of gut microbiota derived-signature for predicting hepatic injuries in schistosomiasis. Here, we characterized the gut microbiomes from 94 human and mouse stool samples using 16S rRNA gene sequencing. Read More

View Article and Full-Text PDF

Integration of allocentric and egocentric visual information in a convolutional/multilayer perceptron network model of goal-directed gaze shifts.

Cereb Cortex Commun 2022 8;3(3):tgac026. Epub 2022 Jul 8.

Centre for Vision Research and Vision: Science to Applications (VISTA) Program, York University, Toronto, Ontario M3J 1P3, Canada.

Allocentric (landmark-centered) and egocentric (eye-centered) visual codes are fundamental for spatial cognition, navigation, and goal-directed movement. Neuroimaging and neurophysiology suggest these codes are initially segregated, but then reintegrated in frontal cortex for movement control. We created and validated a theoretical framework for this process using physiologically constrained inputs and outputs. Read More

View Article and Full-Text PDF

Molecular Detection and Genotyping of in Pigs in Shanxi Province, North China.

Front Vet Sci 2022 15;9:933691. Epub 2022 Jul 15.

College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, China.

is a common opportunistic intestinal pathogen that can cause acute diarrhea in immunosuppressed humans and animals. Though has been widely detected in pigs around the world, little is known of its prevalence and genotype distribution in pigs in Shanxi province, north China. In this study, a total of 362 fecal samples were collected from pigs in three representative counties in north, south, and central Shanxi province, China. Read More

View Article and Full-Text PDF

Malaria prevalence and risk factors among patients visiting Mizan Tepi University Teaching Hospital, Southwest Ethiopia.

PLoS One 2022 28;17(7):e0271771. Epub 2022 Jul 28.

Department of Medical Laboratory Science, College of Health Science and Medicine, Mizan-Tepi University, Mizan-Aman, Ethiopia.

Background: Ethiopia is among sub-Saharan African countries with a high number of malaria cases each year, with most of the landmass favoring the breeding of the vectors. There have been extensive efforts to control and prevent the transmission of malaria, which is part of the country's prevention-based health policy.

Objective: This study aimed to determine malaria prevalence and associated risk factors among patients visiting Mizan-Tepi University Teaching Hospital (MTUTH). Read More

View Article and Full-Text PDF

Preliminary Study on Comparative Efficacy of Four Light Sources for Trapping Culicoides spp. (Diptera: Ceratopogonidae) in Prachuap Khiri Khan Province, Thailand.

J Econ Entomol 2022 Jul 28. Epub 2022 Jul 28.

Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.

The light trap is an important tool to determine the presence and abundance of vectors in the field. However, no one has studied the efficiency of light traps for collecting Culicoides in Thailand. In the present study, the efficacy of four light sources was evaluated in Prachuap Khiri Khan province, Thailand. Read More

View Article and Full-Text PDF

Population genetics and genetic variation of Ctenocephalides felis and Pulex irritans in China by analysis of nuclear and mitochondrial genes.

Parasit Vectors 2022 Jul 27;15(1):266. Epub 2022 Jul 27.

Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China.

Background: Fleas are the most economically significant blood-feeding ectoparasites worldwide. Ctenocephalides felis and Pulex irritans can parasitize various animals closely related to humans and are of high veterinary significance.

Methods: In this study, 82 samples were collected from 7 provinces of China. Read More

View Article and Full-Text PDF

Adaptation to a Multiplex Bead Assay and Seroprevalence to Rift Valley Fever N Protein: Nampula Province, Mozambique, 2013-2014.

J Virol 2022 Jul 27:e0067222. Epub 2022 Jul 27.

Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Preventiongrid.416738.f, Atlanta, Georgia, USA.

Rift Valley fever virus (RVFV) is endemic in sub-Saharan Africa (SSA), with outbreaks reported in the Arabian Peninsula and throughout SSA. The natural reservoir for RVFV are ruminants, with livestock populations exceeding 50% exposure rates in some areas of SSA. Transmission to humans can occur through exposure to infected livestock products or multiple species of mosquito vectors. Read More

View Article and Full-Text PDF

Deep-Learning-Based Estimation of the Spatial QRS-T Angle from Reduced-Lead ECGs.

Sensors (Basel) 2022 Jul 20;22(14). Epub 2022 Jul 20.

Biomedical Engineering Institute, Kaunas University of Technology, 51423 Kaunas, Lithuania.

The spatial QRS-T angle is a promising health indicator for risk stratification of sudden cardiac death (SCD). Thus far, the angle is estimated solely from 12-lead electrocardiogram (ECG) systems uncomfortable for ambulatory monitoring. Methods to estimate QRS-T angles from reduced-lead ECGs registered with consumer healthcare devices would, therefore, facilitate ambulatory monitoring. Read More

View Article and Full-Text PDF

Machine-Learning-Based Fine Tuning of Input Signals for Mechano-Tactile Display.

Sensors (Basel) 2022 Jul 15;22(14). Epub 2022 Jul 15.

Department of Mechanical Engineering, Keio University, Yokohama 223-8522, Kanagawa, Japan.

Deducing the input signal for a tactile display to present the target surface (i.e., solving the inverse problem for tactile displays) is challenging. Read More

View Article and Full-Text PDF

Fantastic AAV Gene Therapy Vectors and How to Find Them-Random Diversification, Rational Design and Machine Learning.

Pathogens 2022 Jul 3;11(7). Epub 2022 Jul 3.

Department of Infectious Diseases/Virology, Medical Faculty, University of Heidelberg, Center for Integrative Infectious Diseases Research (CIID), BioQuant, 69120 Heidelberg, Germany.

Parvoviruses are a diverse family of small, non-enveloped DNA viruses that infect a wide variety of species, tissues and cell types. For over half a century, their intriguing biology and pathophysiology has fueled intensive research aimed at dissecting the underlying viral and cellular mechanisms. Concurrently, their broad host specificity (tropism) has motivated efforts to develop parvoviruses as gene delivery vectors for human cancer or gene therapy applications. Read More

View Article and Full-Text PDF

Differentiation of Laboratory-Obtained × Hybrid Ticks: Selection of Suitable Genes.

Microorganisms 2022 Jun 27;10(7). Epub 2022 Jun 27.

Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS (Institute of Poliomyelitis), 108819 Moscow, Russia.

and ticks are the main vectors of tick-borne encephalitis virus and some bacterial pathogens. The regions where these tick species live overlap, forming large sympatric areas. It has previously been shown that these tick species have no morphological barrier, and interspecies crossing is possible with the appearance of sterile hybrids. Read More

View Article and Full-Text PDF

Subtype of Neuroblastoma Cells with High KIT Expression Are Dependent on KIT and Its Knockdown Induces Compensatory Activation of Pro-Survival Signaling.

Int J Mol Sci 2022 Jul 13;23(14). Epub 2022 Jul 13.

Department of Cancer Cell Biology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.

Neuroblastoma (NB) is a pediatric cancer with high clinical and molecular heterogeneity, and patients with high-risk tumors have limited treatment options. Receptor tyrosine kinase KIT has been identified as a potential marker of high-risk NB and a promising target for NB treatment. We investigated 19,145 tumor RNA expression and molecular pathway activation profiles for 20 cancer types and detected relatively high levels of expression in NB. Read More

View Article and Full-Text PDF