1,701 results match your criteria vasp

Impact of Platelet Reactivity in ACS Patients on Clinical Outcomes with Triple Antithrombotic Therapy.

J Clin Med 2021 Apr 8;10(8). Epub 2021 Apr 8.

Department of Cardiology, ARCHANTEC, School of Medicine, Aix Marseille University, 13007 Marseille, France.

Optimal antithrombotic therapy after percutaneous coronary intervention (PCI) in patients on oral anticoagulants (OAC) remains a clinical conundrum. In fact, combining an OAC with dual antiplatelet therapy (triple antithrombotic therapy, TAT) increases the risk of bleeding. Clopidogrel is the only thienopyridine recommended in TAT patients. Read More

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Spatial proximity of proteins surrounding zyxin under force-bearing conditions.

Mol Biol Cell 2021 Apr 28:mbcE19100568. Epub 2021 Apr 28.

Biomedical Engineering Department, University of California Davis.

Sensing physical forces is a critical first-step in mechano-transduction of cells. Zyxin, a LIM domain-containing protein, is recruited to force-bearing actin filaments and is thought to repair and strengthen them. Yet, the precise force-induced protein interactions surrounding zyxin remain unclear. Read More

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Optimized effective potentials from the random-phase approximation: Accuracy of the quasiparticle approximation.

J Chem Phys 2021 Apr;154(15):154103

Faculty of Physics and Center for Computational Materials Science, University of Vienna, Kolingasse 14-16, A-1090 Vienna, Austria.

The optimized effective potential (OEP) method presents an unambiguous way to construct the Kohn-Sham potential corresponding to a given diagrammatic approximation for the exchange-correlation functional. The OEP from the random-phase approximation (RPA) has played an important role ever since the conception of the OEP formalism. However, the solution of the OEP equation is computationally fairly expensive and has to be done in a self-consistent way. Read More

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Immune cells fold and damage fungal hyphae.

Proc Natl Acad Sci U S A 2021 Apr;118(15)

Aberdeen Fungal Group, Institute of Medical Sciences, Foresterhill, AB25 2ZD Aberdeen, United Kingdom;

Innate immunity provides essential protection against life-threatening fungal infections. However, the outcomes of individual skirmishes between immune cells and fungal pathogens are not a foregone conclusion because some pathogens have evolved mechanisms to evade phagocytic recognition, engulfment, and killing. For example, can escape phagocytosis by activating cellular morphogenesis to form lengthy hyphae that are challenging to engulf. Read More

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Applying Unconventional Spectroscopies to the Single-Molecule Magnets, Co(PPh3)2X2 (X = Cl, Br, I): Unveiling Magnetic Transitions and Spin-Phonon Coupling.

Chemistry 2021 Apr 19. Epub 2021 Apr 19.

Texas A&M University College Station, Department of Chemistry, UNITED STATES.

Large separation of magnetic levels and slow relaxation in metal complexes are desirable properties of single-molecule magnets (SMMs). Spin-phonon coupling (interactions of magnetic levels with phonons) is ubiquitous, leading to magnetic relaxation and loss of memory in SMMs and quantum coherence in qubits. Direct observation of magnetic transitions and spin-phonon coupling in molecules is challenging. Read More

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Derrone Inhibits Platelet Aggregation, Granule Secretion, Thromboxane A Generation, and Clot Retraction: An Study.

Evid Based Complement Alternat Med 2021 10;2021:8855980. Epub 2021 Mar 10.

Department of Biomedical Laboratory Science, Far East University, Eumseong 27601, Republic of Korea.

() is widespread throughout East Asia and in China and Korea, and it is widely used as a traditional remedy against eczema, mumps, and tuberculosis. With regard to the aforementioned medical efficacy, various studies are continuously being conducted, and it has been reported that extract has various actions against inflammation, diabetes, obesity, and tumors. Therefore, we evaluated antiplatelet effects using derrone in We examined the effect of derrone on the phosphorylation of vasodilator-stimulated phosphoprotein (VASP) and inositol 1, 4, 5-triphosphate receptor I (IPRI), and on the dephosphorylation of cytosolic phospholipase A (cPLA), mitogen-activated protein kinases p38 (p38), and Akt, which affects platelet function and thrombus formation. Read More

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Improved Endothelium-Dependent Relaxation of Thoracic Aorta in Niclosamide-Treated Diabetic Rats.

Cardiovasc Toxicol 2021 Mar 27. Epub 2021 Mar 27.

Department of Pharmacology, Faculty of Pharmacy, Karadeniz Technical University, P.O:61080, Trabzon, Turkey.

Diabetes-induced endothelial dysfunction is critical for the development of diabetic cardiovascular complications. The aim of this study was to investigate the effect of niclosamide (Nic) on vascular endothelial dysfunction in streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats were injected with a single intraperitoneal injection of STZ (75 mg/kg) to induce type 1 diabetes, and Nic (10 mg/kg) was intraperitoneally administered per day for 4 weeks. Read More

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PTENε suppresses tumor metastasis through regulation of filopodia formation.

EMBO J 2021 Mar 23:e105806. Epub 2021 Mar 23.

Department of Pathology, School of Basic Medical Sciences, Institute of Systems Biomedicine, Beijing Key Laboratory of Tumor Systems Biology, Peking-Tsinghua Center of Life Sciences, Peking University Health Science Center, Beijing, China.

PTEN is one of the most frequently mutated genes in malignancies and acts as a powerful tumor suppressor. Tumorigenesis is involved in multiple and complex processes including initiation, invasion, and metastasis. The complexity of PTEN function is partially attributed to PTEN family members such as PTENα and PTENβ. Read More

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Role of vasodilator-stimulated phosphoprotein in RANKL-differentiated murine macrophage RAW264.7 cells: Modulation of NF-κB, c-Fos and NFATc1 transcription factors.

Exp Ther Med 2021 May 25;21(5):412. Epub 2021 Feb 25.

Department of Traditional Chinese Traumatology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, Hubei 430061, P.R. China.

Vasodilator-stimulated phosphoprotein (VASP) is essential for osteoclast differentiation, and reduced VASP expression results in depressed osteoclast differentiation. Previously, we demonstrated the importance of VASP and Ras-related C3 botulinum toxin substrate 1 interactions in osteosarcoma cell migration and metastasis using Mg-63 and Saos2 cells. However, the molecular details of the functional role of VASP in cell motility and migration remain to be elucidated. Read More

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A new function for MAP4K4 inhibitors during platelet aggregation and platelet-mediated clot retraction.

Biochem Pharmacol 2021 Mar 15;188:114519. Epub 2021 Mar 15.

Department of Pharmacology and Intractable Disease Research Center, School of Medicine, Dongguk University, Gyeongju, Gyeongsangbuk-do, 38066, Republic of Korea. Electronic address:

Mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4) is implicated in type 2 diabetes mellitus, insulin tolerance, inflammation, cancer, and atherosclerosis. We found that GNE 495 and PF 06260933 (both potent and selective MAP4K4 inhibitors) regulated human platelet activation. Immunoblotting revealed human platelets express MAP4K4, and that GNE 495 and PF 06260933 inhibited collagen-, ADP-, and thrombin-induced platelet aggregation and eventually suppressed granule release, TXA generation, integrin αβ activation, and clot retraction. Read More

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Vasorelaxing cell permeant phosphopeptide mimetics for subarachnoid hemorrhage.

Eur J Pharmacol 2021 Jun 16;900:174038. Epub 2021 Mar 16.

Department of Surgery, Vanderbilt University Medical Center, Nashville, TN, USA. Electronic address:

Subarachnoid hemorrhage (SAH) due to rupture of an intracranial aneurysm leads to vasospasm resulting in delayed cerebral ischemia. Therapeutic options are currently limited to hemodynamic optimization and nimodipine, which have marginal clinical efficacy. Nitric oxide (NO) modulates cerebral blood flow through activation of the cGMP-Protein Kinase G (PKG) pathway. Read More

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NMR structure and dynamics of Q4DY78, a conserved kinetoplasid-specific protein from Trypanosoma cruzi.

J Struct Biol 2021 Mar 9;213(2):107715. Epub 2021 Mar 9.

Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373 - Bloco E, sala 32, Rio de Janeiro, RJ 21941-902, Brazil. Electronic address:

The 106-residue protein Q4DY78 (UniProt accession number) from Trypanosoma cruzi is highly conserved in the related kinetoplastid pathogens Trypanosoma brucei and Leishmania major. Given the essentiality of its orthologue in T. brucei, the high sequence conservation with other trypanosomatid proteins, and the low sequence similarity with mammalian proteins, Q4DY78 is an attractive protein for structural characterization. Read More

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First-principles hydration free energies of oxygenated species at water-platinum interfaces.

J Chem Phys 2021 Mar;154(9):094107

VASP Software GmbH, Sensengasse 8/16, 1090 Vienna, Austria.

The hydration free energy of atoms and molecules adsorbed at liquid-solid interfaces strongly influences the stability and reactivity of solid surfaces. However, its evaluation is challenging in both experiments and theories. In this work, a machine learning aided molecular dynamics method is proposed and applied to oxygen atoms and hydroxyl groups adsorbed on Pt(111) and Pt(100) surfaces in water. Read More

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Nitric Oxide in the Control of the Proliferation and Differentiation of Human Hematopoietic Stem and Progenitor Cells.

Front Cell Dev Biol 2020 9;8:610369. Epub 2021 Feb 9.

Institute of Cell Biology and Biophysics, Leibniz University Hannover, Hannover, Germany.

Hematopoietic stem and progenitor cell (HSPC) transplantation is the best-studied cellular therapy and successful control of HSPCs has wide clinical implications. Nitric oxide (NO) is a central signaling molecule and has been implicated in HSPC mobilization to the blood stream in mice. The influence of NO on HSPC behavior is, however, largely obscure due to the variety of employed cell types, NO administration systems, and used concentration ranges in the literature. Read More

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February 2021

Enabled/VASP is required to mediate proper sealing of opposing cardioblasts during Drosophila dorsal vessel formation.

Dev Dyn 2021 Feb 15. Epub 2021 Feb 15.

Department of Pathology and Laboratory Medicine, Rutgers-Robert Wood Johnson Medical School, Piscataway, New Jersey, USA.

Introduction: The Drosophila dorsal vessel (DV) is comprised of two opposing rows of cardioblasts (CBs) that migrate toward the dorsal midline during development. While approaching the midline, CBs change shape, enabling dorsal and ventral attachments with their contralateral partners to create a linear tube with a central lumen. We previously demonstrated DV closure occurs via a "buttoning" mechanism where specific CBs advance ahead of their lateral neighbors, and attach creating transient holes, which eventually seal. Read More

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February 2021

TRIP6 is required for tension at adherens junctions.

J Cell Sci 2021 Mar 11;134(6). Epub 2021 Mar 11.

Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway NJ 08854, USA

Hippo signaling mediates influences of cytoskeletal tension on organ growth. TRIP6 and LIMD1 have each been identified as being required for tension-dependent inhibition of the Hippo pathway LATS kinases and their recruitment to adherens junctions, but the relationship between TRIP6 and LIMD1 was unknown. Using siRNA-mediated gene knockdown, we show that TRIP6 is required for LIMD1 localization to adherens junctions, whereas LIMD1 is not required for TRIP6 localization. Read More

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Anisotropic Thermoelectric Materials: Pentagonal PtM (M = S, Se, Te).

ACS Appl Mater Interfaces 2021 Feb 8;13(7):8700-8709. Epub 2021 Feb 8.

National Key Laboratory for Shock Wave and Detonation Physics Research, Institute of Fluid Physics, CAEP, Mianyang 621900, China.

We here report a new pentagonal network structure of the PtM (M = S, Se, Te) monolayers with the 2/ (no. 14) space group. The electronic structure and thermoelectric properties of the pentagonal PtM monolayers are calculated through the VASP and BoltzTraP codes. Read More

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February 2021

EVL regulates VEGF receptor-2 internalization and signaling in developmental angiogenesis.

EMBO Rep 2021 Feb 29;22(2):e48961. Epub 2021 Jan 29.

Centre for Molecular Medicine, Institute for Vascular Signalling, Goethe University, Frankfurt am Main, Germany.

Endothelial tip cells are essential for VEGF-induced angiogenesis, but underlying mechanisms are elusive. The Ena/VASP protein family, consisting of EVL, VASP, and Mena, plays a pivotal role in axon guidance. Given that axonal growth cones and endothelial tip cells share many common features, from the morphological to the molecular level, we investigated the role of Ena/VASP proteins in angiogenesis. Read More

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February 2021

Nitrous anhydrase activity of carbonic anhydrase II: cysteine is required for nitric oxide (NO) dependent phosphorylation of VASP in human platelets.

J Enzyme Inhib Med Chem 2021 Dec;36(1):525-534

Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Petersburg, Russia.

The carbonic anhydrase (CA) family does not only catalyse the reversible hydration of CO to bicarbonate, but it also possesses esterase and phosphatase activity. Recently, bovine CA II and human CA II have been reported to convert inorganic nitrite (O=N-O) to nitric oxide (NO) and nitrous anhydride (NO). Given the ability of NO to mediate vasodilation and inhibit platelet aggregation, this CA II activity would represent a bioactivation of nitrite. Read More

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December 2021

Growth factor dependent changes in nanoscale architecture of focal adhesions.

Sci Rep 2021 Jan 27;11(1):2315. Epub 2021 Jan 27.

Department of Pathology, Erasmus Medical Center Rotterdam, Rotterdam, 3015 GE, the Netherlands.

Focal adhesions (FAs) are flat elongated structures that mediate cell migration and link the cytoskeleton to the extracellular matrix. Along the vertical axis FAs were shown to be composed of three layers. We used structured illumination microscopy to examine the longitudinal distribution of four hallmark FA proteins, which we also used as markers for these layers. Read More

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January 2021

Anharmonic Correction to Adsorption Free Energy from DFT-Based MD Using Thermodynamic Integration.

J Chem Theory Comput 2021 Feb 22;17(2):1155-1169. Epub 2021 Jan 22.

Department of Physical and Theoretical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, SK-84215 Bratislava, Slovakia.

Adsorption processes are often governed by weak interactions for which the estimation of entropy contributions by means of the harmonic approximation is prone to be inaccurate. Thermodynamic integration (TI) from the harmonic to the fully interacting system (λ-path integration) can be used to compute anharmonic corrections. Here, we combine TI with (curvilinear) internal coordinates in periodic systems to make the formalism available in computational studies. Read More

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February 2021

Genome-wide discovery of natural variation in pre-mRNA splicing and prioritising causal alternative splicing to salt stress response in rice.

New Phytol 2021 05 14;230(3):1273-1287. Epub 2021 Feb 14.

School of Biological Sciences, University of Nebraska, Lincoln, NE, 68588, USA.

Pre-mRNA splicing is an essential step for the regulation of gene expression. In order to specifically capture splicing variants in plants for genome-wide association studies (GWAS), we developed a software tool to quantify and visualise Variations of Splicing in Population (VaSP). VaSP can quantify splicing variants from short-read RNA-seq datasets and discover genotype-specific splicing (GSS) events, which can be used to prioritise causal pre-mRNA splicing events in GWAS. Read More

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Miconia albicans and Curcuma longa herbal medicines positively modulate joint pain, function and inflammation in patients with osteoarthritis: a clinical study.

Inflammopharmacology 2021 Apr 16;29(2):377-391. Epub 2021 Jan 16.

Instituto de Ciências, Engenharia E Tecnologia, Universidade Federal Dos Vales Do Jequitinhonha E Mucuri, Teófilo Otoni, MG, Brasil.

This study aims to evaluate the analgesic and modulating effect of Curcuma longa and Miconia albicans herbal medicines in knee's osteoarthritis (OA) treatment. This longitudinal study evaluated 24 patients with OA. The patients were divided into three groups: ibuprofen (1200 mg/day), C. Read More

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the Understanding of ACD Toxicity with the Discovery of Cyclic Forms of Actin Oligomers.

Int J Mol Sci 2021 Jan 13;22(2). Epub 2021 Jan 13.

Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.

Actin is an essential element of both innate and adaptive immune systems and can aid in motility and translocation of bacterial pathogens, making it an attractive target for bacterial toxins. Pathogenic and genera deliver actin cross-linking domain (ACD) toxin into the cytoplasm of the host cell to poison actin regulation and promptly induce cell rounding. At early stages of toxicity, ACD covalently cross-links actin monomers into oligomers (AOs) that bind through multivalent interactions and potently inhibit several families of actin assembly proteins. Read More

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January 2021

A cortical immune network map identifies distinct microglial transcriptional programs associated with β-amyloid and Tau pathologies.

Transl Psychiatry 2021 01 14;11(1):50. Epub 2021 Jan 14.

Center for Translational & Computational Neuroimmunology, Department of Neurology, Columbia University Medical Center, New York, NY, USA.

Microglial dysfunction has been proposed as one of the many cellular mechanisms that can contribute to the development of Alzheimer's disease (AD). Here, using a transcriptional network map of the human frontal cortex, we identify five modules of co-expressed genes related to microglia and assess their role in the neuropathologic features of AD in 540 subjects from two cohort studies of brain aging. Two of these transcriptional programs-modules 113 and 114-relate to the accumulation of β-amyloid, while module 5 relates to tau pathology. Read More

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January 2021

3DStructGen: an interactive web-based 3D structure generation for non-periodic molecule and crystal.

J Cheminform 2020 Jan 23;12(1). Epub 2020 Jan 23.

National Supercomputer Center in Guangzhou, School of Data and Computer Science, Sun Yat-sen University, 132 East Circle at University City, Guangzhou, 510006, China.

Background: The increasing number of organic and inorganic structures promotes the development of the "Big Data" in chemistry and material science, and raises the need for cross-platform and web-based methods to search, view and edit structures. Many web-based three-dimensional (3D) structure tools have been developed for displaying existing models, building new models, and preparing initial input files for external calculations. But few of these tools can deal with crystal structures. Read More

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January 2020

[Prevalence of CYP2C19 gene mutations in patients with coronary heart disease and its biological activation effect in clopidogrel antiplatelet response].

Zhonghua Xin Xue Guan Bing Za Zhi 2021 Jan 11;49(1):43-48. Epub 2021 Jan 11.

Department of Cardiovascular Medicine, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China Jiangxi Key Laboratory of Molecular Medicine, Nanchang 330006, China.

The purpose of this study was to investigate the effects of CYP2C19 gene mutations on clopidogrel antiplatelet activity in the patients with coronary heart disease treated by percutaneous coronary intervention. Patients with coronary heart disease, who hospitalized in the Second Affiliated Hospital of Nanchang University from March 2011 to June 2019, and healthy individuals with matching genetic background, gender, and age as controls were included in this study. Basic clinical data were analyzed and blood samples of all research subjects were obtained for extraction of DNA, and Sanger first-generation sequencing method was used to detect CYP2C19 gene mutation from full exon and exon and intron junction. Read More

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January 2021

ab initio study of oxygen vacancy effects on structural, electronic and thermoelectric behavior of AZrMO (A = Ba, Ca, Sr; M= Al, Cu, x = 0.25) for application of memory devices.

J Mol Graph Model 2021 Mar 21;103:107825. Epub 2020 Dec 21.

Division of Electronics and Electrical Engineering, Dongguk University, Seoul, 04620, Republic of Korea.

The structural, electronic and thermoelectric properties of AZrMO (A = Ba, Ca, Sr; M = Al, Cu, x = 0.25) without and with an oxygen vacancy (Vo) have been unveiled using the Perdew-Burke-Ernzerhof Generalized Gradient Approximation (PBE-GGA) functional along with Tran-Blaha modified Becke-Jonhson (TB-mBJ)approximation based on Density Functional Theory (DFT) in the framework of WIEN2k code for memristors applications. Moreover, isosurface charge density plots have been calculated by using Vienna ab initio Simulation Package (VASP) simulation code. Read More

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Machine Learning in Computational Surface Science and Catalysis: Case Studies on Water and Metal-Oxide Interfaces.

Front Chem 2020 30;8:601029. Epub 2020 Nov 30.

Institut für Chemie, Humboldt-Universität zu Berlin, Berlin, Germany.

The goal of many computational physicists and chemists is the ability to bridge the gap between atomistic length scales of about a few multiples of an Ångström (Å), i. e., 10 m, and meso- or macroscopic length scales by virtue of simulations. Read More

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November 2020

Influence of long-term proteasome inhibition on platelet responsiveness mediated by bortezomib.

Vascul Pharmacol 2021 Jan 8:106830. Epub 2021 Jan 8.

Institute of Transfusion Medicine and Haemotherapy, University of Wuerzburg, Oberduerrbacher Straße 6, D-97080 Wuerzburg, Germany. Electronic address:

Introduction: Although platelets contain a full proteasome system, its role in platelet function is not completely understood yet. Since the proteasome system may be involved in time-delayed processes, platelet responsiveness was investigated after long-term, bortezomib-mediated proteasome inhibition.

Materials And Methods: Citrate-anticoagulated whole blood was stored with 5 nM and 1 μM bortezomib for 24 h. Read More

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January 2021