Biomed Pharmacother 2021 Jun 12;140:111762. Epub 2021 Jun 12.
Calcium Signaling Laboratory, Research Service, Veterans Affairs Medical Center, 50 Irving Street, Washington DC 20422, NW, USA; Division of Renal Diseases & Hypertension, Department of Medicine, The George Washington University, Washington DC 20037, USA; Department of Biomedical Engineering, The Catholic University of America, 620 Michigan Avenue, Washington DC 20064, NE, USA. Electronic address:
Vascular calcification is common in chronic kidney disease; however, the extent to which such condition can affect the renal microvasculature and the neighboring cell types is unclear. Our induced-calcification model in renal proximal tubular (PT) cells exhibited endoplasmic reticulum (ER) stress and oxidative damage, leading to apoptosis. Here, we utilized such calcification in mouse vascular smooth muscle (MOVAS-1) cells as a vascular calcification model, because it exhibited reactive oxygen species (ROS) generation, ER and oxidative stress, inflammatory, and apoptotic gene expressions. Read More