17,631 results match your criteria variety characterization

Experimental Evaluation of Shear Behavior of Stone Masonry Wall.

Materials (Basel) 2021 Apr 29;14(9). Epub 2021 Apr 29.

Department of Civil and Industrial Engineering-Structural Division, University of Pisa, 56122 Pisa, Italy.

The evaluation of the shear behavior of masonry walls is a first fundamental step for the assessment of existing masonry structures in seismic zones. However, due to the complexity of modelling experimental behavior and the wide variety of masonry types characterizing historical structures, the definition of masonry's mechanical behavior is still a critical issue. Since the possibility to perform in situ tests is very limited and often conflicting with the needs of preservation, the characterization of shear masonry behavior is generally based on reference values of mechanical properties provided in modern structural codes for recurrent masonry categories. Read More

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Liquid Chromatographic Quadrupole Time-of-Flight Mass Spectrometric Untargeted Profiling of (Poly)phenolic Compounds in L. and L. Fruits and Their Comparative Evaluation.

Antioxidants (Basel) 2021 Apr 29;10(5). Epub 2021 Apr 29.

Department of Chemistry "Ugo Schiff", University of Florence, 50019 Sesto Fiorentino, Italy.

This study provided a detailed profiling of the antioxidant and bioactive compounds occurring in three varieties of L. fruits ("Fall Gold", "Glen Ample" and "Tulameen") compared to L. black raspberry ("Jewel" cultivar), adopting a comprehensive untargeted metabolomics approach developed with UHPLC analysis coupled with quadrupole/time-of-flight high resolution mass spectrometry, using the SWATH acquisition protocol. Read More

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Unveiling the phase behavior of CE non-ionic surfactants in water through coarse-grained molecular dynamics simulations.

Soft Matter 2021 May 4. Epub 2021 May 4.

CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-1933, Aveiro, Portugal.

Poly(oxyethylene) alkyl ethers, usually denoted by CiEj surfactants, exhibit a rich phase behavior in water, self-assembling to form a variety of 3-D structures with a controllable morphology that find multiple applications across different industrial segments. Hence, being able to describe and understand the effect of molecular structure on the phase behavior of these systems is highly relevant for the efficient design of new materials and their applications. Considering the promising results obtained over the last decade using the MARTINI model to describe ethylene-oxide containing compounds, an extensive assessment of the ability of such a model to describe the phase behavior of CiEj in water was carried out and results are presented here. Read More

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Visual cues enhance obstacle avoidance in echolocating bats.

J Exp Biol 2021 May 4;224(9). Epub 2021 May 4.

Department of Psychological and Brain Sciences, Johns Hopkins University, Baltimore, MD 21218, USA.

Studies have shown that bats are capable of using visual information for a variety of purposes, including navigation and foraging, but the relative contributions of visual and auditory modalities in obstacle avoidance has yet to be fully investigated, particularly in laryngeal echolocating bats. A first step requires the characterization of behavioral responses to different combinations of sensory cues. Here, we quantified the behavioral responses of the insectivorous big brown bat, Eptesicus fuscus, in an obstacle avoidance task offering different combinations of auditory and visual cues. Read More

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Locus-specific characterization of human endogenous retrovirus expression in prostate, breast, and colon cancers.

Cancer Res 2021 May 3. Epub 2021 May 3.

Computational Biology Institute, George Washington University

Human endogenous retroviruses (HERV) have been implicated in a variety of diseases including cancers. Recent research implicates HERVs in epigenetic gene regulation. Here we utilize a recently developed bioinformatics tool for identifying HERV expression at the locus-specific level to identify differential expression of HERVs in matched tumor-normal RNA-seq data from The Cancer Genome Atlas (TCGA). Read More

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DNA methylation profiling for molecular classification of adult diffuse lower-grade gliomas.

Clin Epigenetics 2021 May 3;13(1):102. Epub 2021 May 3.

Sahlgrenska Center for Cancer Research, Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Background: DNA methylation profiling has facilitated and improved the classification of a wide variety of tumors of the central nervous system. In this study, we investigated the potential utility of DNA methylation profiling to achieve molecular diagnosis in adult primary diffuse lower-grade glioma (dLGG) according to WHO 2016 classification system. We also evaluated whether methylation profiling could provide improved molecular characterization and identify prognostic differences beyond the classical histological WHO grade together with IDH mutation status and 1p/19q codeletion status. Read More

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Characterization of Anthocyanins From Intraspecific Crosses of Monastrell With Other Premium Varieties.

Front Nutr 2021 16;8:664515. Epub 2021 Apr 16.

Instituto Murciano de Desarrollo Agrario y Alimentario, Murcia, Spain.

One way in which the wine sector is reacting to the challenge of climate change is to develop plant material that is adapted to the new conditions. Such a strategy will allow the continuation of quality viticulture in traditional winemaking areas that will otherwise be abandoned. The objective of this study was to characterize the anthocyanin composition and content of selected intraspecific hybrids of Monastrell with two other varieties (Syrah and Cabernet Sauvignon). Read More

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Phytocannabinomics: Untargeted metabolomics as a tool for cannabis chemovar differentiation.

Talanta 2021 Aug 20;230:122313. Epub 2021 Mar 20.

Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy; CNR NANOTEC, Campus Ecotekne, University of Salento, Via Monteroni, 73100, Lecce, Italy.

Cannabis sativa is traditionally classified according to five chemotypes based on the concentration of the main phytocannabinoids tetrahydrocannabinol (THC), cannabidiol (CBD), and cannabigerol (CBG). However, cannabis chemovars and varieties very often present similar concentrations of such phytocannabinoids but different chemical profiles, which is unavoidably translated into different pharmacological effects when used for therapeutic purposes. For this reason, a more refined approach is needed for chemovar distinction, which is described in this study and named phytocannabinomics. Read More

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Occurrence, distribution, and characterization of suspended microplastics in a highly impacted estuarine wetland in Argentina.

Sci Total Environ 2021 Apr 16;785:147141. Epub 2021 Apr 16.

Instituto Argentino de Oceanografía (IADO), CONICET/UNS, CCT-Bahía Blanca, Camino La Carrindanga, km 7.5, Edificio E1, B8000FWB Bahía Blanca, Buenos Aires, Argentina. Electronic address:

Microplastics have been a global concern due to their potential and widespread risks to organisms and environments. In this study, we investigated the abundance, distribution, and characteristics of microplastics (MPs) in the surface waters of the Bahía Blanca Estuary (BBE), specifically in its inner and middle zone. The results showed the dominant shape of MPs were fibers, being black, transparent, and blue the main colors. Read More

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Preparation and characterization of neutrally-buoyant oleosin-rich synthetic lipid droplets.

Biochim Biophys Acta Biomembr 2021 Apr 30:183624. Epub 2021 Apr 30.

Department of Chemistry, Lehigh University, 6 E. Packer Ave. Bethlehem, PA 18015, USA. Electronic address:

Lipid droplets also known as oil bodies are found in a variety of organisms and function as stores of high-energy metabolites. Recently, there has been interest in using lipid droplets for protein production and drug delivery. Artificial lipid droplets have been previously prepared, but their short lifetime in solution and inhomogeneity has severely limited their applicability. Read More

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Molecular characterization and biological function of CXCR1 in Nocardia seriolae-infected largemouth bass (Micropterus salmoides).

Tissue Cell 2021 Apr 21;72:101551. Epub 2021 Apr 21.

Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China. Electronic address:

Interleukin-8 (IL-8, CXCL8), a pro-inflammatory chemokine secreted by a variety of cell types, plays a critical role in the development of various immune diseases. Interactions between IL-8 and its receptor CXC receptor 1/2 (CXCR1/2) are known to promote chemotaxis and phagocytosis in many immune responses. In this study, we report the molecular characteristics and pharmacological activity of CXCR1 (MsCXCR1) in largemouth bass (Micropterus salmoides) and evaluated the functional involvement of MsCXCR1 in individuals infected with the pathogen Nocardia seriolae. Read More

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Circulating tumor cells as a promising target for individualized drug susceptibility tests in cancer therapy.

Biochem Pharmacol 2021 Apr 28:114589. Epub 2021 Apr 28.

Institute of Biochemistry and Signal Transduction, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. Electronic address:

Circulating tumor cells (CTCs) play a crucial role in metastasis and became an emerging topic in today's cancer research. In addition, the analysis of CTCs in liquid biopsies will be a valuable tool for prognosis prediction and real time therapy monitoring. The characterization of CTCs may open up a new field of treatment strategy to prevent metastasis or maintain a stable disease. Read More

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Characterization of a novel toll-like receptor and activation NF-κB signal pathway in triangle sail mussel Hyriopsis cumingii.

Comp Biochem Physiol B Biochem Mol Biol 2021 Apr 27;255:110608. Epub 2021 Apr 27.

College of Life Sciences, Department of Aquatic Sciences, Nanchang University, Nanchang 330031, China. Electronic address:

Toll-like receptor (TLR) family plays an important role in innate immunity for detection of and defense against microbial pathogens. In this study, a novel toll-like receptor (HcTLRn) was characterized from freshwater pearl mussel H. cumingii. Read More

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Glycosyl Oxocarbenium Ions: Structure, Conformation, Reactivity, and Interactions.

Acc Chem Res 2021 Apr 30. Epub 2021 Apr 30.

CIC bioGUNE, Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Building 800, 48160 Derio, Spain.

ConspectusCarbohydrates (glycans, saccharides, and sugars) are essential molecules in all domains of life. Research on glycoscience spans from chemistry to biomedicine, including material science and biotechnology. Access to pure and well-defined complex glycans using synthetic methods depends on the success of the employed glycosylation reaction. Read More

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Culture Substrates for Improved Manufacture of Mesenchymal Stromal Cell Therapies.

Adv Healthc Mater 2021 Apr 30:e2100016. Epub 2021 Apr 30.

Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, GA, 30332, USA.

Recent developments in mesenchymal stromal cell (MSC) therapies have increased the demand for tools to improve their manufacture, including the selection of optimal culture substrate materials. While many clinical manufacturers use planar tissue culture plastic (TCP) surfaces for MSC production, others have begun exploring the use of alternative culture substrates that present a variety of spatial, mechanical, and biochemical cues that influence cell expansion and resulting cell quality. In this review, the effects of culture and material properties distinct from traditional planar TCP surfaces on MSC proliferation, surface marker expression, and commonly used indications for therapeutic potency are examined. Read More

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Cloning, expression, and characterization of Baeyer-Villiger monooxygenases from eukaryotic Exophiala jeanselmei strain KUFI-6N.

Biosci Biotechnol Biochem 2021 Apr 30. Epub 2021 Apr 30.

Department of Life Science & Biotechnology, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.

The fungus Exophiala jeanselmei strain KUFI-6N produces a unique cycloalkanone monooxygenase (ExCAMO) that displays an uncommon substrate spectrum of Baeyer-Villiger oxidation of 4-10-membered ring ketones. In this study, we aimed to identify and sequence the gene encoding ExCAMO from KUFI-6N and overexpress the gene in Escherichia coli. We found that the primary structure of ExCAMO is most closely related to the cycloalkanone monooxygenase from Cylindrocarpon radicicola ATCC 11011, with 54. Read More

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Genomic and transcriptomic characterization of desmoplastic small round cell tumors.

Genes Chromosomes Cancer 2021 Apr 30. Epub 2021 Apr 30.

Division of Clinical Genetics, Department of Laboratory Medicine, Lund University, Lund, Sweden.

Desmoplastic small round cell tumor (DSRCT) is a highly aggressive soft tissue tumor primarily affecting children and young adults. Most cases display a pathognomonic EWSR1-WT1 gene fusion, presumably constituting the primary driver event. Little is, however, known about secondary genetic changes that may affect tumor progression. Read More

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Identification of Human Antigen-Specific CD4+ Cells with Peptide-MHC Multimer Technologies.

Methods Mol Biol 2021 ;2285:153-163

The Benaroya Research Institute at Virginia Mason, Seattle, WA, USA.

Peptide-major histocompatibility complex class II (pMHCII) multimers have emerged as a convenient and powerful tool for characterization of CD4 T cell immune responses in a large variety of human diseases. Peptide-MHCII multimers can rapidly identify peptide antigens recognized by CD4 T cells via high-throughput peptide screening procedures. The specificity and phenotype of antigen-specific CD4 T cells can be effectively visualized by pMHCII multimers from unmanipulated immune cell populations. Read More

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January 2021

Styrene-Acrylic Emulsion with "Transition Layer" for Damping Coating: Synthesis and Characterization.

Polymers (Basel) 2021 Apr 27;13(9). Epub 2021 Apr 27.

Key Lab of Advanced Technology for Specially Functional Materials, Ministry of Education, and School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, China.

In order to study the dynamic mechanical properties of styrene-acrylic latex with a core/shell structure, a variety of latexes were synthesized by semi-continuous seeded emulsion polymerization based on "particle design" with the same material. The latexes were characterized by rotary viscosimeter, dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), dynamic mechanical analysis (DMA), and universal testing machine. The effects of difference at the glass transition temperature () of core and shell and the introduction of the "transition layer" on the damping and mechanical properties of latex film were studied. Read More

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Entropy of the Land Parcel Mosaic as a Measure of the Degree of Urbanization.

Entropy (Basel) 2021 Apr 28;23(5). Epub 2021 Apr 28.

Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.

Quantifying the urbanization level is an essential yet challenging task in urban studies because of the high complexity of this phenomenon. The urbanization degree has been estimated using a variety of social, economic, and spatial measures. Among the spatial characteristics, the Shannon entropy of the landscape pattern has recently been intensively explored as one of the most effective urbanization indexes. Read More

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Identification and Characterization of Abiotic Stress Responsive CBL-CIPK Family Genes in .

Int J Mol Sci 2021 Apr 28;22(9). Epub 2021 Apr 28.

Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

The calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK) play important roles in plant signal transduction and response to abiotic stress. Plants of genus contain many important forages, and their growth is often affected by a variety of abiotic stresses. However, studies on the CBL and CIPK family member and their function are rare in . Read More

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Recent Advances in Sequential Infiltration Synthesis (SIS) of Block Copolymers (BCPs).

Nanomaterials (Basel) 2021 Apr 13;11(4). Epub 2021 Apr 13.

Advanced Materials and Life Sciences, Istituto Nazionale di Ricerca Metrologica (INRiM), Strada delle Cacce 91, 10135 Turin, Italy.

In the continuous downscaling of device features, the microelectronics industry is facing the intrinsic limits of conventional lithographic techniques. The development of new synthetic approaches for large-scale nanopatterned materials with enhanced performances is therefore required in the pursuit of the fabrication of next-generation devices. Self-assembled materials as block copolymers (BCPs) provide great control on the definition of nanopatterns, promising to be ideal candidates as templates for the selective incorporation of a variety of inorganic materials when combined with sequential infiltration synthesis (SIS). Read More

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Design and Characterization of Electrochemical Sensor for the Determination of Mercury(II) Ion in Real Samples Based upon a New Schiff Base Derivative as an Ionophore.

Sensors (Basel) 2021 Apr 25;21(9). Epub 2021 Apr 25.

Department of Chemistry, University College in Al-Jamoum, Umm Al-Qura University, Makkah 21955, Saudi Arabia.

The present paper provides a description of the design, characterization, and use of a Hg selective electrode (Hg-SE) for the determination of Hg at ultra-traces levels in a variety of real samples. The ionophore in the proposed electrode is a new Schiff base, namely 4-bromo-2-[(4-methoxyphenylimino)methyl]phenol (BMPMP). All factors affecting electrode response including polymeric membrane composition, concentration of internal solution, pH sample solution, and response time were optimized. Read More

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Validated Stability-Indicating GC-MS Method for Characterization of Forced Degradation Products of -Caffeic Acid and -Ferulic Acid.

Molecules 2021 Apr 23;26(9). Epub 2021 Apr 23.

Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna Pot 113, SI-1000 Ljubljana, Slovenia.

When dealing with simple phenols such as caffeic acid (CA) and ferulic acid (FA), found in a variety of plants, it is very important to have control over the most important factors that accelerate their degradation reactions. This is the first report in which the stabilities of these two compounds have been systematically tested by exposure to various different factors. Forced degradation studies were performed on pure standards (-CA and -FA), dissolved in different solvents and exposed to different oxidative, photolytic and thermal stress conditions. Read More

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Genome-Wide Analysis and Expression Profiles of the Dof Family in under Temperature, Salt and ABA Treatment.

Plants (Basel) 2021 Apr 23;10(5). Epub 2021 Apr 23.

The State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.

The DNA-binding with one zinc finger () family of plant-specific transcription factors has a variety of important functions in gene transcriptional regulation, development, and stress responses. However, the structure and expression patterns of family have not been identified in , which is an important xerophytic and perennial gramineous grass in desert grassland. In this study, 50 genes were identified in and could be classified into four groups. Read More

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Down-Regulation of in Tomato Confers Abiotic Stress Tolerance.

Genes (Basel) 2021 Apr 22;12(5). Epub 2021 Apr 22.

College of Life Sciences, Henan Normal University, Xinxiang 453007, China.

Adverse environmental factors like salt stress, drought, and extreme temperatures, cause damage to plant growth, development, and crop yield. GRAS transcription factors (TFs) have numerous functions in biological processes. Some studies have reported that the GRAS protein family plays significant functions in plant growth and development under abiotic stresses. Read More

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First Insight into Nutraceutical Properties of Local Salento Varieties: NMR-Based Metabolomic Approach.

Int J Environ Res Public Health 2021 Apr 12;18(8). Epub 2021 Apr 12.

Department of Biological and Environmental Sciences and Technologies, University of Salento, Prov.le Lecce-Monteroni, 73100 Lecce, Italy.

Background: Plants of genus are known for their therapeutic and nutraceutical properties determined by a wealth of phytochemical substances contained in the whole plant. The aim of this paper was to characterize the metabolic profiles of local Salento chicory ( L.) varieties ("Bianca", "Galatina", "Leccese", and "Otranto") in order to describe their metabolites composition together with possible bioactivity and health beneficial properties. Read More

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Biological Properties and Genetic Characterization of Novel Low Pathogenic H7N3 Avian Influenza Viruses Isolated from Mallard Ducks in the Caspian Region, Dagestan, Russia.

Microorganisms 2021 Apr 17;9(4). Epub 2021 Apr 17.

Federal State Budget Scientific Institution, Federal Research Center of Fundamental and Translational Medicine, 630117 Novosibirsk, Russia.

Avian influenza viruses (AIVs) are maintained in wild bird reservoirs, particularly in mallard ducks and other waterfowl. Novel evolutionary lineages of AIV that arise through genetic drift or reassortment can spread with wild bird migrations to new regions, infect a wide variety of resident bird species, and spillover to domestic poultry. The vast continental reservoir of AIVs in Eurasia harbors a wide diversity of influenza subtypes, including both highly pathogenic (HP) and low pathogenic (LP) H7 AIV. Read More

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Elemental Characterization of Ciders and Other Low-Percentage Alcoholic Beverages Available on the Polish Market.

Molecules 2021 Apr 10;26(8). Epub 2021 Apr 10.

Faculty of Chemistry, Institute of General and Ecological Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.

Seventy-three samples of alcoholic beverages and juices that were purchased on the Polish market and home-made were analyzed for their elemental profiles. The levels of 23 metals were determined by ICP-MS (Ag, Ba, Bi, Cd, Co, Cr, Li, Mn, Ni, Pb, Sr and Tl), ICP-OES (Al, B, Ca, Cu, Fe, K, Mg, Na, Ti and Zn) and CVAAS (Hg) techniques in twenty-five samples of ciders widely available on the Polish market; six samples of home-made ciders; two samples of juices used in the production of these ciders; and forty samples of low-percentage, flavored alcoholic beverages based on beer. The gathered analytical data confirmed that the final elemental fingerprint of a product is affected by the elemental fingerprint of the ingredients used (apple variety) as well as the technology and equipment used by the producer, and in the case of commercial ciders, also the impact of type of the packaging used was proven. Read More

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Phytochemical Characterization and Screening of Antioxidant, Antimicrobial and Antiproliferative Properties of Clementi and Two Varieties of L. Peel Extracts.

Plants (Basel) 2021 Apr 21;10(5). Epub 2021 Apr 21.

Department of Biology, Faculty of Science, University of Split, R. Boškovića 33, 21000 Split, Croatia.

Onions are one of the most widely grown vegetable crops. As production increases, so does the generation of waste from various parts of the onion, raising the need for efficient ecological disposal and use of such waste products. However, onion waste products are a rich source of antioxidants with a range of biological properties, therefore, they could potentially be used in food and pharmaceutical industries. Read More

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