51,921 results match your criteria varied cellular

Polymorphic tandem DNA repeats activate the human telomerase reverse transcriptase gene.

Proc Natl Acad Sci U S A 2021 Jun;118(26)

College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, WA 99210;

Multiple independent sequence variants of the locus have been associated with telomere length and cancer risks in genome-wide association studies. Here, we identified an intronic variable number tandem repeat, VNTR2-1, as an enhancer-like element, which activated hTERT transcription in a cell in a chromatin-dependent manner. VNTR2-1, consisting of 42-bp repeats with an array of enhancer boxes, cooperated with the proximal promoter in the regulation of hTERT transcription by basic helix-loop-helix transcription factors and maintained hTERT expression during embryonic stem-cell differentiation. Read More

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Metasurface-assisted broadband optical absorption in ultrathin perovskite films.

Opt Express 2021 Jun;29(12):19170-19182

Ultrathin hybrid organic-inorganic perovskite (HOIP) films have significant potential for use in integrated high-performance photoelectric devices. However, the relatively low optical absorption capabilities of thinner films, particularly in the long-wavelength region, pose a significant challenge to the further improvement of photoelectrical conversion in ultrathin HOIP films. To address this problem, we propose a combining of ultrathin HOIP film with plasmonic metasurface to enhance the absorption of the film effectively. Read More

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T cell transgressions: tales of T cell form and function in diversedisease states.

Int Rev Immunol 2021 Jun 21:1-42. Epub 2021 Jun 21.

Department of Psychology and Neuroscience, Center for Neuroscience, University of Colorado, Boulder, Colorado, USA.

Insights into T cell form, function, and dysfunction are rapidly evolving. T cells have remarkably varied effector functions including protecting the host from infection, activating cells of the innate immune system, releasing cytokines and chemokines, and heavily contributing to immunological memory. Under healthy conditions, T cells orchestrate a finely tuned attack on invading pathogens while minimizing damage to the host. Read More

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Control Release of Adenosine Potentiate Osteogenic Differentiation within a Bone Integrative EGCG--NOCC/Collagen Composite Scaffold toward Guided Bone Regeneration in a Critical-Sized Calvarial Defect.

Biomacromolecules 2021 Jun 21. Epub 2021 Jun 21.

Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.

The regeneration of critical-sized bone defects with biomimetic scaffolds remains clinically challenging due to avascular necrosis, chronic inflammation, and altered osteogenic activity. Two confounding mechanisms, efficacy manipulation, and temporal regulation dictate the scaffold's bone regenerative ability. Equally critical is the priming of the mesenchymal stromal cells (MSCs) toward lineage-specific differentiation into bone-forming osteoblast, which particularly depends on varied mechanochemical and biological cues during bone tissue regeneration. Read More

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Posttranslational Modifications of the Cytoskeleton.

Cytoskeleton (Hoboken) 2021 Jun 21. Epub 2021 Jun 21.

Department of Biomedical Sciences, University of Pennsylvania, School of Veterinary Medicine, Philadelphia, PA.

The cytoskeleton plays important roles in many essential processes at the cellular and organismal levels, including cell migration and motility, cell division, and the establishment and maintenance of cell and tissue architecture. In order to facilitate these varied functions, the main cytoskeletal components - microtubules, actin filaments, and intermediate filaments - must form highly diverse intracellular arrays in different subcellular areas and cell types. The question of how this diversity is conferred has been the focus of research for decades. Read More

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Genomic and properties of the dairy SMQ-301 strain against selected pathogens.

Food Funct 2021 Jun 21. Epub 2021 Jun 21.

Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China.

Cumulative studies have suggested that probiotic bacterial strains could be an effective alternative in inhibiting conditions caused by foodborne and vaginal pathogens. The use of genomic techniques is becoming highly useful in understanding the potential of these beneficial microorganisms. This study presents some genomic and in vitro properties of the Streptococcus thermophilus SMQ-301 strain against foodborne and vaginal pathogens (Staphylococcus aureus, Escherichia coli, and Gardnerella vaginalis) to validate its use in dairy food formulations. Read More

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Serum HBV pregenomic RNA exhibited opposite associations with NKdim and NKbright cell immunity in treatment-naïve chronic hepatitis B patients.

Biosci Rep 2021 Jun 21. Epub 2021 Jun 21.

Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a new biomarker that reflects HBV replication, but its relationship with natural killer (NK) cell immunity in chronic hepatitis B (CHB) is unknown. We assessed serum HBV pgRNA levels in 323 CHB patients by reverse transcription-polymerase chain reaction, assessed cytokine production and activation and inhibitory markers of NK cells by flow cytometry, and measured serum cytokines by enzyme-linked immunosorbent assays. Among the different CHB phases, the serum HBV pgRNA level was highest in the immune tolerant (IT) and immune active (IA) phases. Read More

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The toxicity of SiO NPs on cell proliferation and cellular uptake of human lung fibroblastic cell line during the variation of calcination temperature and its modeling by artificial neural network.

J Environ Health Sci Eng 2021 Jun 30;19(1):985-995. Epub 2021 Apr 30.

UR4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, Dunkerque, France.

Less attention had been paid to cell toxicity of the various synthesis methods of nanoparticles, this study investigated the effect of the calcination temperature(CT) on the crystallization of SiO nanoparticles(NPs), cell proliferation(CP), and cellular uptake(CU) in MRC-5. In this study, parameters were adjusted as CT(70-1000 °C), calcination time(2, 12, and 24 h), and catalyst feed rate(0.01, 0. Read More

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Epigenetic Features of HIV-Induced T-Cell Exhaustion Persist Despite Early Antiretroviral Therapy.

Front Immunol 2021 4;12:647688. Epub 2021 Jun 4.

Peter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.

T cell dysfunction occurs early following HIV infection, impacting the emergence of non-AIDS morbidities and limiting curative efforts. ART initiated during primary HIV infection (PHI) can reverse this dysfunction, but the extent of recovery is unknown. We studied 66 HIV-infected individuals treated from early PHI with up to three years of ART. Read More

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Pattern formation in drying blood drops.

Philos Trans A Math Phys Eng Sci 2021 Aug 21;379(2203):20200391. Epub 2021 Jun 21.

BioPRIA and Department of Chemical Engineering, Monash University, Clayton, Victoria 3800, Australia.

Patterns in dried droplets are commonly observed as rings left after spills of dirty water or coffee have evaporated. Patterns are also seen in dried blood droplets and the patterns have been shown to differ from patients afflicted with different medical conditions. This has been proposed as the basis for a new generation of low-cost blood diagnostics. Read More

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Polyphenol-assisted facile assembly of bioactive nanoparticles for targeted therapy of heart diseases.

Biomaterials 2021 Jun 9;275:120952. Epub 2021 Jun 9.

Department of Pharmaceutics, College of Pharmacy, Third Military Medical University (Army Medical University), Chongqing 400038, China; State Key Lab of Trauma, Burn and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China. Electronic address:

It remains a great challenge for targeted therapy of heart diseases. To achieve desirable heart targeting, we developed a polyphenol-assisted nanoprecipitation/self-assembly approach for facile engineering of functional nanoparticles. Three different materials were employed as representative carriers, while gallic acid, catechin, epigallocatechin gallate, and tannic acid (TA) served as typical polyphenols with varied numbers of phenolic hydroxyl groups. Read More

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Factors that regulate expression patterns of insulin-like peptides and their association with physiological and metabolic traits in Drosophila.

Insect Biochem Mol Biol 2021 Jun 16:103609. Epub 2021 Jun 16.

Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine; Research and Development University, Ivano-Frankivsk, Ukraine. Electronic address:

Insulin-like peptides (ILPs) and components of the insulin signaling pathway are conserved across different animal phyla. Eight ILPs (called DILPs) and two receptors, dInR and Lgr3, have been described in Drosophila. DILPs regulate varied physiological traits including lifespan, reproduction, development, feeding behavior, stress resistance and metabolism. Read More

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Evaluations for surrounding tissue incorporation after implantation of synthetic vascular prostheses in animal models.

J Artif Organs 2021 Jun 18. Epub 2021 Jun 18.

Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan.

Incorporation of surrounding tissues after implantation of synthetic vascular prostheses potentially varies in accordance with implanted prostheses. To evaluate post-implant tissue incorporation, we examined surgical, histological and ultrastructural findings after implantation in animal models. Three types of commercially available prostheses were tested (Gelweave™; Group G, J Graft SHIELD NEO; Group J and Triplex; Group T). Read More

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Cationic liposomes mediated transdermal delivery of meloxicam and ketoprofen: optimization of the composition, in vitro and in vivo assessment of efficiency.

Int J Pharm 2021 Jun 15:120803. Epub 2021 Jun 15.

Arbuzov Institute of Organic and Physical Chemistry, FRC Kazan Scientific Center of RAS, 8 Arbuzov str., 420088, Kazan, Russian Federation.

New liposomes modified by pyrrolidinium surfactants containing a hydroxyethyl fragment (CPB, n = 12, 14, 16) were prepared for transdermal delivery of non-steroidal anti-inflammatory drugs. In order to obtain the optimal composition, the surfactant/lipid molar ratio (0.02/1; 0. Read More

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Toll-Like Receptor 4 as a Favorable Prognostic Marker in Bladder Cancer: A Multi-Omics Analysis.

Front Cell Dev Biol 2021 1;9:651560. Epub 2021 Jun 1.

Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: The toll-like receptor 4 (TLR4) agonist, Bacille Calmette-Guérin, has exhibited gratifying effects in treating bladder cancer. The study aims to explore the expression pattern, prognostic value, and potential mechanism of TLR4 in bladder cancer.

Methods: The transcriptome file from the GSE13507 dataset in the Gene Expression Omnibus database and the promoter methylation file from the bladder cancer dataset in The Cancer Genome Atlas database were downloaded for analysis. Read More

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Pancreatic Intraductal Papillary Mucinous Neoplasm with Hyaline Globules (Thanatosomes): Report of Two Cases.

Int Med Case Rep J 2021 10;14:393-399. Epub 2021 Jun 10.

Department of Diagnostic Pathology, Shimada City General Medical Center, Shimada, Shizuoka, Japan.

Hyaline globules (HGs; thanatosomes) represent a morphologic entity representing a metabolic imbalance common to all cell types. HGs, intracytoplasmic eosinophilic globular accumulations of proteinaceous material of varying sizes, have been observed in varied tumors and benign tissues. Different explanations have been proposed for their formation, according to the tumor type and anatomic location. Read More

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Massive PD-L1 and CD8 double positive TILs characterize an immunosuppressive microenvironment with high mutational burden in lung cancer.

J Immunother Cancer 2021 Jun;9(6)

Department of Thoracic Surgery, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China

Background: Programmed cell death ligand 1 (PD-L1) expressed on tumor and immune cells are both associated with the response to programmed cell death 1 (PD-1) pathway blockade therapy. Here, we examine the role of CD8+PD-L1+ tumor-infiltrating lymphocyte (Tils) in the tumor microenvironment of non-small cell lung cancer (NSCLC).

Methods: Tumor tissue samples of a total of 378 patients from two NSCLC cohorts were collected retrospectively. Read More

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Subclassification of the Bethesda Category III (AUS/FLUS): A Study of thyroid FNA cytology based on ThinPrep slides from the National Cancer Center in China.

Cancer Cytopathol 2021 Jun 17. Epub 2021 Jun 17.

Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: The atypia of an undetermined significance/follicular lesion of undetermined significance (AUS/FLUS) category in the Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) is a heterogeneous category, which includes various cell patterns. The subclassification of AUS/FLUS was suggested in the 2017 TBSRTC. However, the risk of malignancy (ROM) associated with different subgroups remains unresolved. Read More

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Transient mechanical interactions between cells and viscoelastic extracellular matrix.

Soft Matter 2021 Jun 17. Epub 2021 Jun 17.

Weldon School of Biomedical Engineering, Purdue University, 206 S Martin Jischke Dr, West Lafayette, IN 47907, USA.

During various physiological processes, such as wound healing and cell migration, cells continuously interact mechanically with a surrounding extracellular matrix (ECM). Contractile forces generated by the actin cytoskeleton are transmitted to a surrounding ECM, resulting in structural remodeling of the ECM. To better understand how matrix remodeling takes place, a myriad of in vitro experiments and simulations have been performed during recent decades. Read More

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The cytotoxic and apoptotic effects of beta-blockers with different selectivity on cancerous and healthy lung cell lines.

Mol Biol Rep 2021 Jun 16. Epub 2021 Jun 16.

Department of Physiology, Faculty of Medicine, Pamukkale University, Kinikli, 20020, Denizli, Turkey.

β-blockers having specific affinities to β-adrenergic receptors are routinely used to treat cardiovascular problems. Additionally, it has been demonstrated that these drugs can be effective in treating apoptosis-related diseases. The current study was conducted to investigate the cytotoxic and apoptotic effects of β-1 selective esmolol, β-2 selective ICI-118,551, and non-selective nadolol blockers on the cancerous and healthy lung cells. Read More

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Designing and Development of FRET-Based Nanosensor for Real Time Analysis of N-Acetyl-5-Neuraminic Acid in Living Cells.

Front Nutr 2021 31;8:621273. Epub 2021 May 31.

Department of Botany, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India.

-acetyl-5-neuraminic acid (NeuAc) plays crucial role in improving the growth, brain development, brain health maintenance, and immunity enhancement of infants. Commercially, it is used in the production of antiviral drugs, infant milk formulas, cosmetics, dietary supplements, and pharmaceutical products. Because of the rapidly increasing demand, metabolic engineering approach has attracted increasing attention for NeuAc biosynthesis. Read More

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Partial disintegration of vegetable cell wall during cooking improves vitamin K1 (Phylloquinone) bioaccessibility in digestion.

Int J Vitam Nutr Res 2021 Jun 17:1-12. Epub 2021 Jun 17.

UMR QualiSud, Université de Montpellier, CIRAD, Institut Agro, Université d'Avignon, Université de la Réunion, Montpellier, France.

Vegetables rich in vitamin K consumption could prevent bleeding and maintain bone status. The aims of the present work were to investigate i) the effect of household cooking (i.e. Read More

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In silico screening and exploration into phenotypic alterations of deleterious oncogenic single nucleotide polymorphisms in HSPB1 gene.

Genomics 2021 Jun 12. Epub 2021 Jun 12.

Amity Institute of Biotechnolgy, Amity University Kolkata, India. Electronic address:

A small heat shock protein, HSP27, encoded by HSPB1 gene strongly favors survival, proliferation and metastasis of cancer cells and its expression is dependent on post-translational modifications like phosphorylation. This study performed an extensive in silico screening of 20 deleterious non-synonymous SNPs in the coding region of HSPB1 gene, among which four were identified to be cancer associated. The SNP variant I181S introduced a new phosphorylation site in position 181, which might elevate the protein's activation potential. Read More

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Telmisartan Attenuates the Growth of Epithelium-like Cells and Glomerular Injury in Spontaneously Hypertensive Rats.

Curr Med Sci 2021 Jun 15. Epub 2021 Jun 15.

State Key Laboratory of Biotherapy and Cancer Center, Sichuan University, Chengdu, 610041, China.

The abnormal growth of epithelium-like cells has been noticed in spontaneously hypertensive rats (SHRs) with hypertensive nephropathy. However, the characteristics of abnormal epithelium-like cells and their pathogenesis in hypertensive nephropathy are not fully understood. In the present study, we investigated the correlation of epithelium-like cells with glomerular injury, and the effects of early drug intervention with telmisartan, an anti-hypertensive drug, on the growth of epithelium-like cells. Read More

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Absolute Quantification of MicroRNAs in a Single Cell with Chemiluminescence Detection Based on Rolling Circle Amplification on a Microchip Platform.

Anal Chem 2021 Jun 15. Epub 2021 Jun 15.

State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541004, China.

The absolute quantification of miRNAs in a single cell allows to better understand the heterogeneity of cells and the relationship between miRNAs and diseases. However, seldom methods for miRNA quantification in a single cell have been reported because the miRNA content in a single cell is very low. Herein, an ultrasensitive chemiluminescence assay strategy based on rolling circle amplification (RCA) on a microchip platform was proposed for the absolute quantification of miRNAs in a single cell. Read More

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Genes with 5' terminal oligopyrimidine tracts preferentially escape global suppression of translation by the SARS-CoV-2 NSP1 protein.

RNA 2021 Jun 14. Epub 2021 Jun 14.

University of Texas at Austin

Viruses rely on the host translation machinery to synthesize their own proteins. Consequently, they have evolved varied mechanisms to co-opt host translation for their survival. SARS-CoV-2 relies on a non-structural protein, Nsp1, for shutting down host translation. Read More

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2-Amino-3'-dialkylaminobiphenyl-based fluorescent intracellular probes for nitric oxide surrogate NO.

Chem Sci 2020 Jan 2;11(5):1394-1403. Epub 2020 Jan 2.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology Meilong Road 130 Shanghai 200237 China

Fluorescent probes for nitric oxide (NO), or more frequently for its oxidized surrogate dinitrogen trioxide (NO), have enabled scientists to study the contributions of this signaling molecule to many physiological processes. Seeking to improve upon limitations of other probes, we have developed a family of fluorescent probes based on a 2-amino-3'-dialkylaminobiphenyl core. This core condenses with NO to form benzo[]cinnoline structures, incorporating the analyte into the newly formed fluorophore, which results in product fluorescence with virtually no background contribution from the initial probe. Read More

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January 2020

Interplay between intrinsically disordered proteins inside membraneless protein liquid droplets.

Chem Sci 2019 Dec 11;11(5):1269-1275. Epub 2019 Dec 11.

Department of Chemistry, KAIST 291 Daehak-ro, Yuseong-gu Daejeon 34143 Republic of Korea +82-42-350-2810 +82-42-350-2817.

Intrinsically disordered proteins (IDPs) in cells phase separate to form diverse membraneless organelles, which have condensed liquid droplet-like properties and often contain multiple IDPs. However, how potential interactions between different IDPs affect the dynamic behavior of these protein droplets is largely unknown. Here, we develop a rapid IDP clustering system to generate protein droplets with varied residue compositions and examine diverse interacting IDPs inside droplets. Read More

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December 2019

Recent structural evolution of lactam- and imide-functionalized polymers applied in organic field-effect transistors and organic solar cells.

Chem Sci 2021 May 7;12(20):6844-6878. Epub 2021 May 7.

Beijing National Laboratory for Molecular Sciences, CAS Research/Education Centre for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences Beijing 100190 P. R. China

Organic semiconductor materials, especially donor-acceptor (D-A) polymers, have been increasingly applied in organic optoelectronic devices, such as organic field-effect transistors (OFETs) and organic solar cells (OSCs). Plenty of high-performance OFETs and OSCs have been achieved based on varieties of structurally modified D-A polymers. As the basic building block of D-A polymers, acceptor moieties have drawn much attention. Read More

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Revealing the Immune Infiltration Landscape and Identifying Diagnostic Biomarkers for Lumbar Disc Herniation.

Front Immunol 2021 27;12:666355. Epub 2021 May 27.

Department of Orthopedic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Intervertebral disc (IVD) degeneration and its inflammatory microenvironment ultimately led to discogenic pain, which is thought to originate in the nucleus pulposus (NP). In this study, key genes involved in NP tissue immune infiltration in lumbar disc herniation (LDH) were identified by bioinformatic analysis. Gene expression profiles were downloaded from the Gene Expression Omnibus (GEO) database. Read More

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