183,320 results match your criteria validated study


LC-MS/MS method for simultaneous quantification of the first-line anti-tuberculosis drugs and six primary metabolites in patient plasma: Implications for therapeutic drug monitoring.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Oct 14;1185:122986. Epub 2021 Oct 14.

Latvian Biomedical Research and Study Centre, Ratsupites Street 1, k-1, Riga, LV1067, Latvia; Riga Stradins University, Dzirciema Street 16, Riga, LV1007, Latvia. Electronic address:

The pharmacokinetic profiling of drug substances and corresponding metabolites in the biological matrix is one of the most informative tools for the treatment efficacy assessment. Therefore, to satisfy the need for comprehensive monitoring of anti-tuberculosis drugs in human plasma, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous quantification of first-line anti-tuberculosis drugs (ethambutol, isoniazid, pyrazinamide, and rifampicin) along with their six primary metabolites. Simple single-step protein precipitation with methanol was chosen as the most convenient sample pre-treatment method. Read More

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October 2021

Determination of morphine, codeine, and thebaine concentrations from poppy seed tea using magnetic carbon nanotubes facilitated dispersive micro-solid phase extraction and GC-MS analysis.

Forensic Sci Int 2021 Oct 12;329:111052. Epub 2021 Oct 12.

Department of Forensic Science, Sam Houston State University, 1003 Bowers Blvd, Huntsville, TX, USA. Electronic address:

With tightening enforcement and restrictions amid the opioid epidemic, poppy seed tea is consumed as an alternative to mitigate the withdrawal symptoms or as a home remedy to relieve pain and stress. Previously published studies suggested the potential danger of consuming tea brewed with a moderate to a large amount of poppy seed. In this study, the effects of small quantity and repeat brewing on opiate concentrations were evaluated. Read More

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October 2021

Development of a Preoperative Clinical Risk Assessment Tool for Postoperative Complications After Hysterectomy.

J Minim Invasive Gynecol 2021 Oct 20. Epub 2021 Oct 20.

the Department of Obstetrics and Gynecology, University of Michigan, 1500 E. Medical Center Dr., Ann Arbor, MI 48109 USA. Electronic address:

Study Objective: To develop a preoperative risk assessment tool that quantifies the risk of postoperative complications within 30 days of hysterectomy.

Design: Retrospective analysis.

Setting: Michigan Surgical Quality Collaborative hospitals. Read More

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October 2021

Alzheimer's disease associated with Down syndrome: a genetic form of dementia.

Lancet Neurol 2021 Nov;20(11):930-942

Sant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu y Sant Pau, Biomedical Research Institute Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain; Barcelona Down Medical Center, Fundació Catalana Síndrome de Down, Barcelona, Spain; Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas. CIBERNED, Madrid, Spain.

Adults with Down syndrome develop the neuropathological hallmarks of Alzheimer's disease and are at very high risk of developing early-onset dementia, which is now the leading cause of death in this population. Diagnosis of dementia remains a clinical challenge because of the lack of validated diagnostic criteria in this population, and because symptoms are overshadowed by the intellectual disability associated with Down syndrome. In people with Down syndrome, fluid and imaging biomarkers have shown good diagnostic performances and a strikingly similar temporality of changes with respect to sporadic and autosomal dominant Alzheimer's disease. Read More

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November 2021

BIOMARKERS OF GASTROINTESTINAL DISEASE IN CHEETAHS ().

J Zoo Wildl Med 2021 Sep;52(3):886-892

Gastrointestinal Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 4474 TAMU, College Station, TX 77843, USA.

Gastrointestinal disease is a common clinical problem in captive cheetahs (). It is reported that gastritis affects the vast majority of the captive population of cheetahs. Pancreatitis and acute and chronic enteritis have also been reported. Read More

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September 2021

A Novel Adenoidectomy Training System.

Laryngoscope 2021 Oct 23. Epub 2021 Oct 23.

Departments of Pediatrics and Otolaryngology-Head and Neck Surgery, Lewis Katz School of Medicine at Temple University, Philadelphia, Pennsylvania, U.S.A.

Objectives/hypothesis: Young residents find mirror-guided adenoidectomy difficult. Inexperienced trainees must learn to focus a headlight beam, work upside-down and backward in a small space and thoroughly ablate adenoid tissue-all new skills. We present an adenoidectomy training system that is low-cost, easy to construct, and is focused on these basic adenoidectomy skills. Read More

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October 2021

Lactate and pyruvate levels correlation with lactate dehydrogenase gene expression and glucose consumption in Tamoxifen resistant MCF-7 cells using capillary electrophoresis with contactless conductivity detection (CE-C D).

Electrophoresis 2021 Oct 23. Epub 2021 Oct 23.

Department of Analytical Chemistry, Faculty of Pharmacy, Helwan University, Cairo, Egypt.

Breast cancer is the second leading cause of cancer death in women after lung cancer. The first line treatment of metastatic breast cancer in premenopausal women relies on tamoxifen. The development of tamoxifen resistance is not fully understood. Read More

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October 2021

Identifying a Patient-Centered Outcome Measure for a Comparative Effectiveness Treatment Trial in Myasthenia Gravis.

Muscle Nerve 2021 Oct 23. Epub 2021 Oct 23.

Duke University Medical Center, Durham, NC.

Introduction/aim: Data regarding the comparative effectiveness of myasthenia gravis (MG) treatments is not available. We used patient input to identify a patient-centered outcome measure (PCOM) for PROMISE-MG, a comparative effectiveness trial of myasthenia gravis (MG) treatments.

Methods: 1. Read More

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October 2021

Low prevalence of neuropathic-like pain symptoms in long-term controlled acromegaly.

Pituitary 2021 Oct 23. Epub 2021 Oct 23.

Department of Medicine, Division of Endocrinology, and Center for Endocrine Tumors Leiden, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands.

Purpose: Pain is a common symptom of acromegaly, impairing health-related quality of life (HR-QoL) significantly despite long-term disease remission. Neuropathic-like pain (NP-like) symptoms are invalidating, with great impact on HR-QoL. Studies characterizing or investigating the etiology of pain in acromegaly are scarce. Read More

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October 2021

Functional abnormality in the sensorimotor system attributed to NRXN1 variants in boys with attention deficit hyperactivity disorder.

Brain Imaging Behav 2021 Oct 23. Epub 2021 Oct 23.

Peking University Sixth Hospital, Peking University Institute of Mental Health, NHC Key Laboratory of Mental Health (Peking University), National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Huayuan Bei Road 51, Haidian District, Beijing, 100191, China.

Impaired sensorimotor circuits have been suggested in Attention-deficit/hyperactivity disorder (ADHD). NRXN1, highly expressed in cortex and cerebellum, was one of the candidate risk genes for ADHD, while its effects on sensorimotor circuits are unclear. In this content, we aimed to investigate the differential brain effects as functions of the cumulative genetic effects of NRXN1 variants in ADHD and healthy controls (HCs), identifying a potential pathway mapping from NRXN1, sensorimotor circuits, to ADHD. Read More

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October 2021

Application of comprehensive bioinformatics approaches to reconnoiter crucial genes and pathways underpinning hepatocellular carcinoma: a drug repurposing endeavor.

Med Oncol 2021 Oct 23;38(12):145. Epub 2021 Oct 23.

Department of Biotechnology, M. S. Ramaiah Institute of Technology, Bengaluru, Karnataka, 560054, India.

Hepatocellular carcinoma (HCC) is the fifth most common neoplasm in the world. Chronic inflammation of liver and associated wound healing processes collectively contribute to the development of cirrhosis which further progresses to dysplastic nodule and then to HCC. Etiological mediators and ongoing manipulations at cellular level in HCC are well established; however, key protein interactions and genetic alterations involved in stepwise hepatocarcinogenic pathways are seldom explored. Read More

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October 2021

Application of the anatomical fiducials framework to a clinical dataset of patients with Parkinson's disease.

Brain Struct Funct 2021 Oct 23. Epub 2021 Oct 23.

Department of Clinical Neurological Sciences, London Health Sciences Centre, Western University, London, ON, Canada.

Establishing spatial correspondence between subject and template images is necessary in neuroimaging research and clinical applications such as brain mapping and stereotactic neurosurgery. Our anatomical fiducial (AFID) framework has recently been validated to serve as a quantitative measure of image registration based on salient anatomical features. In this study, we sought to apply the AFIDs protocol to the clinic, focusing on structural magnetic resonance images obtained from patients with Parkinson's disease (PD). Read More

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October 2021

Longitudinal associations of fiber, vegetable, and fruit intake with quality of life and fatigue in colorectal cancer survivors up to 24 months post-treatment.

Am J Clin Nutr 2021 Oct 23. Epub 2021 Oct 23.

Department of Epidemiology, GROW School for Oncology and Developmental Biology, Maastricht University, Maastricht, the Netherlands.

Background: The increasing colorectal cancer (CRC) survivor population highlights the need for dietary recommendations in order to enhance health-related quality of life (HRQoL) and alleviate symptoms of fatigue, chemotherapy-induced peripheral neuropathy (CIPN) and gastro-intestinal problems.

Objectives: Because of the therapeutic potential of dietary fiber on the gut, we aim to assess longitudinal associations of post-diagnostic dietary fiber, fruit and vegetable intake, a major source of dietary fiber, with HRQoL, fatigue, CIPN and gastro-intestinal symptoms in CRC survivors from 6 weeks to 24 months post-treatment.

Methods: In a prospective cohort among stage I-III CRC survivors (n = 459), five repeated study measurements between diagnosis and 24 months post-treatment were executed. Read More

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October 2021

Unplanned admission to pediatric intensive care after general anesthesia: A seven-year retrospective cohort study in a tertiary children's hospital.

Paediatr Anaesth 2021 Oct 22. Epub 2021 Oct 22.

Department of Critical Care and Anesthesia, National Center for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo, 157-8535, Japan.

Background: Thorough preoperative risk assessment and planning is key to improving patient safety in the perioperative period. Analysis of unplanned ICU admissions after general anesthesia has been validated as a measure of patient safety and its use as a quality initiative is recommended in many countries.

Aims: The aims of this study were to determine the reasons for unplanned ICU admission, required interventions, and outcomes after general anesthesia in our hospital, as well as predictability and preventability of the events that led to admission with a view to improving anesthetic management. Read More

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October 2021

A DLPNO-CCSD(T) benchmarking study of intermolecular interactions of ionic liquids.

J Comput Chem 2021 Oct 23. Epub 2021 Oct 23.

School of Chemistry, Monash University, Clayton, Victoria, Australia.

The accuracy of correlation energy recovered by coupled cluster single-, double-, and perturbative triple-excitations, CCSD(T), has led to the method being considered the gold standard of computational chemistry. The application of CCSD(T) has been limited to medium-sized molecular systems due to its steep scaling with molecular size. The recent development of alternative domain-based local pair natural orbital coupled-cluster method, DLPNO-CCSD(T), has significantly broadened the range of chemical systems to which CCSD(T) level calculations can be applied. Read More

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October 2021

Novel methods of microbiome analysis in the food industry.

Int Microbiol 2021 Oct 23. Epub 2021 Oct 23.

Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias-Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Paseo Río Linares s/n, 33300, Villaviciosa, Asturias, Spain.

The study of the food microbiome has gained considerable interest in recent years, mainly due to the wide range of applications that can be derived from the analysis of metagenomes. Among these applications, it is worth mentioning the possibility of using metagenomic analyses to determine food authenticity, to assess the microbiological safety of foods thanks to the detection and tracking of pathogens, antibiotic resistance genes and other undesirable traits, as well to identify the microorganisms responsible for food processing defects. Metataxonomics and metagenomics are currently the gold standard methodologies to explore the full potential of metagenomes in the food industry. Read More

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October 2021

Posterior Urethral Valves Outcomes Prediction (PUVOP): a machine learning tool to predict clinically relevant outcomes in boys with posterior urethral valves.

Pediatr Nephrol 2021 Oct 22. Epub 2021 Oct 22.

Division of Urology, Department of Surgery, University of Toronto, Toronto, ON, Canada.

Background: Early kidney and anatomic features may be predictive of future progression and need for additional procedures in patients with posterior urethral valve (PUV). The objective of this study was to use machine learning (ML) to predict clinically relevant outcomes in these patients.

Methods: Patients diagnosed with PUV with kidney function measurements at our institution between 2000 and 2020 were included. Read More

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October 2021

Optimized Method for Determination 57 Volatile Organic Compounds in Nitrogen Using GC × GC-FID.

J Chromatogr Sci 2021 Oct 23. Epub 2021 Oct 23.

Institute for Environmental Reference Materials, Environmental Development Center of the Ministry of Ecology and Environment, No. 1 Yuhui Road, Chaoyang, Beijing, 10029, China.

The sample containing 57 volatile organic compounds (VOCs) in nitrogen at a nominal 1 ppmv was prepared in our lab using weighting method. A methodology for determination of the 57 VOCs using a two-dimensional gas chromatography equipped with Deans switches and two flame ionization detectors (GC × GC-FID) was developed and validated for resolution, asymmetry, sensitivity, precision (intra-day precision and inter-day precision), linearity, limit of detection (LOD), limit of quantification (LOQ) and accuracy. In this study, resolution, asymmetry and sensitivity of the analytical method were improved,intra-day precisions of all the compounds were <1% and inter-day precisions were between 0. Read More

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October 2021

Ga-DOTATOC PET/CT to detect immune checkpoint inhibitor-related myocarditis.

J Immunother Cancer 2021 Oct;9(10)

Immunology and allergy division, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland

Background: Immune checkpoint inhibitor (ICI)-related myocarditis is a rare but potentially fatal adverse event that can occur following ICI exposure. Early diagnosis and treatment are key to improve patient outcomes. Somatostatin receptor-based positron emission tomography-CT (PET/CT) showed promising results for the assessment of myocardial inflammation, yet information regarding its value for the diagnosis of ICI-related myocarditis, especially at the early stage, is limited. Read More

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October 2021

Exploration of Predictive Modeling of Defense Health Agency & Department of Veterans Affairs Beneficiaries Dietary Supplements Use.

J Perianesth Nurs 2021 Oct 19. Epub 2021 Oct 19.

United States Army Graduate Program in Anesthesia Nursing, Fort Sam Houston, TX. Electronic address:

Purpose: The purpose of this study was to predict dietary supplements (DS) use among Defense Health Agency (DHA) and Veterans Affairs (VA) beneficiaries who were scheduled for surgical procedures.

Design: This was a predictive descriptive multicenter study with a convenience sample of 2,623 adult subjects. After institutional review board exemption, data collection occurred preoperatively via electronic survey. Read More

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October 2021

Pan-cancer analysis of non-coding transcripts reveals the prognostic onco-lncRNA HOXA10-AS in gliomas.

Cell Rep 2021 Oct;37(3):109873

Ontario Institute for Cancer Research, Toronto, ON, Canada; Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada. Electronic address:

Long non-coding RNAs (lncRNAs) are increasingly recognized as functional units in cancer and powerful biomarkers; however, most remain uncharacterized. Here, we analyze 5,592 prognostic lncRNAs in 9,446 cancers of 30 types using machine learning. We identify 166 lncRNAs whose expression correlates with survival and improves the accuracy of common clinical variables, molecular features, and cancer subtypes. Read More

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October 2021

Expanded regulation of circular RNA translation.

Mol Cell 2021 Oct;81(20):4111-4113

State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China; School of Life Science and Technology, ShanghaiTech University, 100 Haike Road, Shanghai 201210, China; School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China. Electronic address:

Chen et al. (2021) have identified many internal ribosome entry site-like elements that can potentially drive circRNA translation. Dozens of such element-containing circRNAs-encoded peptides are validated, among which a circFGFR1-encoded protein acts as an antagonist of FGFR1. Read More

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October 2021

Canine Non-Angiogenic, Non-Myogenic Splenic Stromal Sarcoma: a Retrospective Clinicopathological Analysis and Investigation of Podoplanin as a Marker of Tumour Histogenesis.

J Comp Pathol 2021 Oct 27;188:1-12. Epub 2021 Aug 27.

Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA; Flint Animal Cancer Center, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA. Electronic address:

Splenic stromal sarcomas are rarely reported tumours that were previously grouped as non-angiomatous, non-lymphomatous mesenchymal neoplasms of the canine spleen. Highly variable survival times have been reported probably due to their heterogeneous nature. The purpose of this study was to assess the outcome and prognostic factors in dogs with splenic stromal sarcoma after treatment by splenectomy. Read More

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October 2021

  Extract: Antioxidant and Cytotoxic Activities Supported by Chemical Composition and Molecular Docking Simulations.

Plants (Basel) 2021 Oct 11;10(10). Epub 2021 Oct 11.

Department of Chemistry, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt.

The current study was designed to investigate the antioxidant and cytotoxic activities of whole-plant extract. The total phenolic content was determined using Folin-Ciocalteu reagent and found to be 980.1 mg/g, calculated as gallic acid equivalents. Read More

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October 2021

QTL Validation and Development of SNP-Based High Throughput Molecular Markers Targeting a Genomic Region Conferring Narrow Root Cone Angle in Aerobic Rice Production Systems.

Plants (Basel) 2021 Oct 3;10(10). Epub 2021 Oct 3.

School of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD 4072, Australia.

Aerobic rice production (AP) provides potential solutions to the global water crisis by consuming less water than traditional permanent water culture. Narrow root cone angle (RCA), development of deeper rooting and associated genomic regions are key for AP adaptation. However, their usefulness depends on validation across genetic backgrounds and development of linked markers. Read More

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October 2021

Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study.

Cells 2021 Oct 13;10(10). Epub 2021 Oct 13.

Department of Epidemiology and Prevention, IRCCS NEUROMED, 86077 Pozzilli, Italy.

Defined as an index of platelet size heterogeneity, the (PDW) is still a poorly characterized marker of platelet function in (sub)clinical disease. We presently validated PDW as a marker of P-selectin dependent platelet activation in the Moli-family cohort. Platelet-bound P-selectin and platelet/leukocyte mixed aggregates were measured by flow cytometry in freshly collected venous blood, both before and after in vitro platelet activation, and coagulation time was assessed in unstimulated and LPS- or TNFα-stimulated whole blood. Read More

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October 2021

Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Advanced Non-Small Cell Lung Cancer after Neoadjuvant Therapy.

Cells 2021 Oct 13;10(10). Epub 2021 Oct 13.

Institute of Pathology, University of Bern, 3008 Bern, Switzerland.

In recent years autophagy has attracted the attention of researchers from many medical fields, including cancer research, and certain anti-macroautophagy drugs in combination with cytotoxic or targeted therapies have entered clinical trials. In the present study, we focused on a less explored subtype of autophagy, i.e. Read More

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October 2021

Mapping the Proximity Interaction Network of STIM1 Reveals New Mechanisms of Cytoskeletal Regulation.

Cells 2021 Oct 9;10(10). Epub 2021 Oct 9.

Health Sciences Center, Department of Physiology, University of Tennessee, Memphis, TN 38163, USA.

Stromal interaction molecule 1 (STIM1) resides primarily in the sarco/endoplasmic reticulum, where it senses intraluminal Ca levels and activates Orai channels on the plasma membrane to initiate Ca influx. We have previously shown that STIM1 is involved in the dynamic remodeling of the actin cytoskeleton. However, the downstream effectors of STIM1 that lead to cytoskeletal remodeling are not known. Read More

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October 2021

Investigation of Neurodevelopmental Deficits of 22 q11.2 Deletion Syndrome with a Patient-iPSC-Derived Blood-Brain Barrier Model.

Cells 2021 Sep 28;10(10). Epub 2021 Sep 28.

Department of Biomedical Engineering, The City College of the City University of New York, New York, NY 10031, USA.

The blood-brain barrier (BBB) is important in the normal functioning of the central nervous system. An altered BBB has been described in various neuropsychiatric disorders, including schizophrenia. However, the cellular and molecular mechanisms of such alterations remain unclear. Read More

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September 2021

Nano-Infrared Imaging of Primary Neurons.

Cells 2021 Sep 27;10(10). Epub 2021 Sep 27.

Medical Microspectroscopy, Department of Experimental Medical Science, Lund University, 22180 Lund, Sweden.

Alzheimer's disease (AD) accounts for about 70% of neurodegenerative diseases and is a cause of cognitive decline and death for one-third of seniors. AD is currently underdiagnosed, and it cannot be effectively prevented. Aggregation of amyloid-β (Aβ) proteins has been linked to the development of AD, and it has been established that, under pathological conditions, Aβ proteins undergo structural changes to form β-sheet structures that are considered neurotoxic. Read More

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September 2021