1,982 results match your criteria vaccines preserved


Neutrophil Cells Are Essential for The Efficacy of a Therapeutic Vaccine against Paracoccidioidomycosis.

J Fungi (Basel) 2021 May 26;7(6). Epub 2021 May 26.

Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.

Paracoccidioidomycosis (PCM), caused by the species, is a systemic disease endemic in several Latin American countries, mainly in Brazil, Colombia, Argentina, and Venezuela. Current treatment approaches are challenging as they require prolonged durations of antifungal drugs that have potential toxicities, and despite antifungals, relapses are common. Hence, new therapeutic approaches, such as vaccines, are being investigated. Read More

View Article and Full-Text PDF

Immunisation program reviews in East and Southern Africa (2012-2018): key lessons.

Pan Afr Med J 2021 22;38:194. Epub 2021 Feb 22.

World Health Organisation, Inter-Country Support Team for East and Southern Africa, Harare, Zimbabwe.

Introduction: the World Health Organisation (WHO) recommends that countries conduct comprehensive national immunisation program reviews regularly to help them identify systems wide-barriers or gaps and monitor performance against the set targets.

Methods: we reviewed reports from the latest national immunisation program reviews conducted in the 20 countries in the sub-region in the course of 2012-2018. We generated descriptive analysis of the findings across the sub-region. Read More

View Article and Full-Text PDF

Immunogenicity of COVID-19 mRNA Vaccines in Pregnant and Lactating Women.

JAMA 2021 May 13. Epub 2021 May 13.

Harvard Medical School, Boston, Massachusetts.

Importance: Pregnant women are at increased risk of morbidity and mortality from COVID-19 but have been excluded from the phase 3 COVID-19 vaccine trials. Data on vaccine safety and immunogenicity in these populations are therefore limited.

Objective: To evaluate the immunogenicity of COVID-19 messenger RNA (mRNA) vaccines in pregnant and lactating women, including against emerging SARS-CoV-2 variants of concern. Read More

View Article and Full-Text PDF

Comprehensive mapping of neutralizing antibodies against SARS-CoV-2 variants induced by natural infection or vaccination.

medRxiv 2021 May 5. Epub 2021 May 5.

Background: Immunity after SARS-CoV-2 infection or vaccination has been threatened by recently emerged SARS-CoV-2 variants. A systematic summary of the landscape of neutralizing antibodies against emerging variants is needed.

Methods: We systematically searched PubMed, Embase, Web of Science, and 3 pre-print servers for studies that evaluated neutralizing antibodies titers induced by previous infection or vaccination against SARS-CoV-2 variants and comprehensively collected individual data. Read More

View Article and Full-Text PDF

Thermostable, Dissolvable Buccal Film Rotavirus Vaccine Is Highly Effective in Neonatal Gnotobiotic Pig Challenge Model.

Vaccines (Basel) 2021 Apr 30;9(5). Epub 2021 Apr 30.

Universal Stabilization Technologies, Inc., San Diego, CA 92121, USA.

Difficulties related to storage and transport of currently available live oral rotavirus vaccines can have detrimental consequences on the efficacy of the vaccines. Thus, there is a great need for thermostable vaccines that can eliminate the necessity for cold chain storage or reconstitution before administration. In this study, we developed a dissolvable oral polymeric film comprised of a live attenuated thermostable tetravalent rhesus-human reassortant rotavirus vaccine (RRV-TV) powder and antacid (CaCO). Read More

View Article and Full-Text PDF

The C-Terminal Domain of Nef Is Dispensable for the CD8 T Cell Immunogenicity of In Vivo Engineered Extracellular Vesicles.

Vaccines (Basel) 2021 Apr 12;9(4). Epub 2021 Apr 12.

National Center for Global Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.

Intramuscular injection of DNA vectors expressing the extracellular vesicle (EV)-anchoring protein Nef fused at its C-terminus to viral and tumor antigens elicit a potent, effective, and anti-tolerogenic CD8 T cell immunity against the heterologous antigen. The immune response is induced through the production of EVs incorporating Nef-derivatives released by muscle cells. In the perspective of a possible translation into the clinic of the Nef-based vaccine platform, we aimed at increasing its safety profile by identifying the minimal part of Nef retaining the EV-anchoring protein property. Read More

View Article and Full-Text PDF

Plasma-Activated Medium Potentiates the Immunogenicity of Tumor Cell Lysates for Dendritic Cell-Based Cancer Vaccines.

Cancers (Basel) 2021 Apr 1;13(7). Epub 2021 Apr 1.

Department for Immunology and Immunoparasitology, Institute for the Application of Nuclear Energy, University of Belgrade, 11080 Belgrade, Serbia.

Autologous dendritic cells (DCs)-based vaccines are considered quite promising for cancer immunotherapy due to their exquisite potential to induce tumor antigen-specific cytotoxic T cells. However, a lack of efficient protocols for inducing immunogenic tumor antigens limits the efficacy of DC-based cancer vaccines. Here, we found that a plasma-activated medium (PAM) induces immunogenic cell death (ICD) in tumor cells but not in an immortalized L929 cell line or human peripheral blood mononuclear cells. Read More

View Article and Full-Text PDF

[Evaluation on monitoring effect of the electronic vaccine vial monitor label].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2021 Feb;38(1):154-160

Beijing Zhongyi Health Research Institute, Beijing 100089, P.R.China.

The cold chain safety of vaccines is a global issue. The electronic vaccine vial monitor (eVVM) label can monitor the temperature of vaccines in real time and provide "early warning" prompts. In order to comprehensively evaluate the monitoring efficiency of eVVM, this study selected 75 eVVM labels and distributed them with a total of 600 vaccine vial monitor (VVM) labels of four different types in different experimental environment (2-8℃, -20℃ and 40℃), and used a temperature recorder as "gold standard". Read More

View Article and Full-Text PDF
February 2021

Methylene blue photochemical treatment as a reliable SARS-CoV-2 plasma virus inactivation method for blood safety and convalescent plasma therapy for COVID-19.

BMC Infect Dis 2021 Apr 16;21(1):357. Epub 2021 Apr 16.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Disease, National Medical Center for Infectious Disease, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University , Hangzhou, 310003, China.

Background: In 2020, a new coronavirus, SARS-CoV-2, quickly spread worldwide within a few months. Although coronaviruses typically infect the upper or lower respiratory tract, the virus RNA can be detected in plasma. The risk of transmitting coronavirus via transfusion of blood products remains. Read More

View Article and Full-Text PDF

Oligomerization and Cell Egress Controlled by Two Microdomains of Canine Distemper Virus Matrix Protein.

mSphere 2021 Apr 14;6(2). Epub 2021 Apr 14.

Division of Experimental Clinical Research, DCR-VPH, Vetsuisse Faculty, University of Bern, Bern, Switzerland

The multimeric matrix (M) protein of clinically relevant paramyxoviruses orchestrates assembly and budding activity of viral particles at the plasma membrane (PM). We identified within the canine distemper virus (CDV) M protein two microdomains, potentially assuming α-helix structures, which are essential for membrane budding activity. Remarkably, while two rationally designed microdomain M mutants (E89R, microdomain 1 and L239D, microdomain 2) preserved proper folding, dimerization, interaction with the nucleocapsid protein, localization at and deformation of the PM, the virus-like particle formation, as well as production of infectious virions (as monitored using a membrane budding-complementation system), were, in sharp contrast, strongly impaired. Read More

View Article and Full-Text PDF

On the use of adenovirus dodecahedron as a carrier for glycoconjugate vaccines.

Glycoconj J 2021 Apr 14. Epub 2021 Apr 14.

Unité Fonctionnalité et Ingénierie des Protéines (UFIP), Université de Nantes, CNRS, UMR 6286, F-44000, Nantes, France.

Virus-Like Particles (VLPs) have been used as immunogenic molecules in numerous recombinant vaccines. VLPs can also serve as vaccine platform to exogenous antigens, usually peptides incorporated within the protein sequences which compose the VLPs or conjugated to them. We herein described the conjugation of a synthetic tetrasaccharide mimicking the Streptococcus pneumoniae serotype 14 capsular polysaccharide to recombinant adenoviral type 3 dodecahedron, formed by the self-assembling of twelve penton bases and investigated the induced immune response when administered subcutaneously (s. Read More

View Article and Full-Text PDF

Lyophilized yeast powder for adjuvant free thermostable vaccine delivery.

Appl Microbiol Biotechnol 2021 Apr 9;105(8):3131-3143. Epub 2021 Apr 9.

Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, 94158, USA.

Thermolabile nature of commercially available vaccines necessitates their storage, transportation, and dissemination under refrigerated condition. Maintenance of continuous cold chain at every step increases the final cost of vaccines. Any breach in the cold chain even for a short duration results in the need to discard the vaccines. Read More

View Article and Full-Text PDF

Role of Electrostatic Hotspots in the Selectivity of Complement Control Proteins Toward Human and Bovine Complement Inhibition.

Front Mol Biosci 2021 16;8:618068. Epub 2021 Mar 16.

Department of Bioengineering, University of California, Riverside, CA.

Poxviruses are dangerous pathogens, which can cause fatal infection in unvaccinated individuals. The causative agent of smallpox in humans, , is closely related to the bovine , yet the molecular basis of their selectivity is currently incompletely understood. Here, we examine the role of the electrostatics in the selectivity of the smallpox protein SPICE and vaccinia protein VCP toward the human and bovine complement protein C3b, a key component of the complement immune response. Read More

View Article and Full-Text PDF

Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens.

Vaccines (Basel) 2021 Mar 12;9(3). Epub 2021 Mar 12.

Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeonsangbuk-do, Korea.

A local virus isolate, O/SKR/JC/2014 (O JC), has been considered as a candidate vaccine strain in the development of a domestic foot-and-mouth disease (FMD) vaccine in Korea. However, producing and preserving a sufficient quantity of intact vaccine antigens from the O JC strain was difficult owing to its distinctive structural instability compared to other candidate vaccine strains. Based on this feature, the O JC strain was adopted as a model virus for the stabilization study to determine the optimal stabilizer composition, which enables long-term storage of the FMD vaccine antigen in both aqueous and frozen phases. Read More

View Article and Full-Text PDF

Immunisation program reviews in East and Southern Africa: 2012-2018; key lessons.

Pan Afr Med J 2020 30;37:385. Epub 2020 Dec 30.

World Health Organisation, Inter-Country Support Team for East and Southern Africa, Harare, Zimbabwe.

Introduction: World Health Organisation (WHO) recommends that countries conduct comprehensive national immunisation programme reviews regularly to help them identify systems wide-barriers or gaps and monitor performance against the set targets.

Methods: we reviewed reports from the latest national immunisation program reviews conducted in the 20 countries in the subregion in the course of 2012-2018. We generated descriptive analysis of the findings across the subregion. Read More

View Article and Full-Text PDF

COVID-19 vaccines and vaccine administration.

Br J Nurs 2021 Mar;30(6):344-349

Volunteer Vaccinator, East Durham Primary Care Network and Professor of Nursing, Northumbria University, Newcastle upon Tyne.

This article provides an overview of current COVID-19 vaccines available within the UK, including their mode of action, storage and handling. It outlines the recommendations on priority groups for vaccination and provides insight into the training recommendations for vaccinators. Read More

View Article and Full-Text PDF

A review on the interaction of nucleoside analogues with SARS-CoV-2 RNA dependent RNA polymerase.

Int J Biol Macromol 2021 Jun 22;181:605-611. Epub 2021 Mar 22.

Department of Nanotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Electronic address:

The outbreaks of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) in 2019, have highlighted the concerns about the lack of potential vaccines or antivirals approved for inhibition of CoVs infection. SARS-CoV-2 RNA dependent RNA polymerase (RdRp) which is almost preserved across different viral species can be a potential target for development of antiviral drugs, including nucleoside analogues (NA). However, ExoN proofreading activity of CoVs leads to their protection from several NAs. Read More

View Article and Full-Text PDF

The potential effects of deploying SARS-Cov-2 vaccines on cold storage capacity and immunization workload in countries of the WHO African Region.

Vaccine 2021 04 19;39(15):2165-2176. Epub 2021 Feb 19.

Center for Vaccine Development and Global Health, 685 W. Baltimore St., University of Maryland School of Medicine, Baltimore, MD, USA. Electronic address:

Background: SARS-CoV-2 vaccines will be deployed to countries with limited immunization systems.

Methods: We assessed the effect of deploying SARS-Cov-2 vaccines on cold storage capacity and immunization workload in a simulated WHO African Region country using region-specific data on immunization, population, healthcare workers (HCWs), cold storage capacity (quartile values for national and subnational levels), and characteristics of an approved SARS-CoV-2 vaccine. We calculated monthly increases in vaccine doses, doses per vaccinator, and cold storage volumes for four-month SARS-CoV-2 vaccination campaigns targeting risk groups compared to routine immunization baselines. Read More

View Article and Full-Text PDF

Latent cytomegalovirus infection and previous capsular polysaccharide vaccination predict poor vaccine responses in older adults, independent of chronic kidney disease.

Clin Infect Dis 2021 Mar 17. Epub 2021 Mar 17.

Institute of Clinical Sciences, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham, UK.

Background: Patients with chronic kidney disease (CKD) are more prone to severe infection. Vaccination is a key strategy to reduce this risk. Some studies suggest vaccine efficacy may be reduced in patients with CKD, despite preserved maintenance of long-term responses to some pathogens and vaccines. Read More

View Article and Full-Text PDF

Organoid Polymer Functionality and Mode of Membrane Antigen Presentation Regulates Germinal Center Epigenetics in Young and Aged B Cells.

Adv Funct Mater 2020 Nov 3;30(48). Epub 2020 Jun 3.

Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY.

Antibiotic-resistant bacteria are a major global health threat that continues to rise due to a lack of effective vaccines. Of concern are that fail to induce germinal center B cell responses, which facilitate antibody production to fight infection. Immunotherapies using antibodies targeting antibiotic-resistant bacteria are emerging as promising alternatives, however, they cannot be efficiently derived , necessitating the need for immune technologies to develop therapeutics. Read More

View Article and Full-Text PDF
November 2020

Human neutralizing antibodies against SARS-CoV-2 require intact Fc effector functions for optimal therapeutic protection.

Cell 2021 04 12;184(7):1804-1820.e16. Epub 2021 Feb 12.

Department of Medicine, Washington University School of Medicine, St. Louis, St. Louis, MO 63110, USA; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, St. Louis, MO 63110, USA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, St. Louis, MO 63110, USA; The Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St. Louis, St. Louis, MO 63110, USA. Electronic address:

SARS-CoV-2 has caused the global COVID-19 pandemic. Although passively delivered neutralizing antibodies against SARS-CoV-2 show promise in clinical trials, their mechanism of action in vivo is incompletely understood. Here, we define correlates of protection of neutralizing human monoclonal antibodies (mAbs) in SARS-CoV-2-infected animals. Read More

View Article and Full-Text PDF

A comparative assessment of cold chain management using the outbreak of circulating vaccine-derived polio virus type 2 as a surrogate marker in Oyo State, Nigeria-2019.

Pan Afr Med J 2020 3;37:313. Epub 2020 Dec 3.

Nigeria Field Epidemiology and Laboratory Programme, Asokoro, Abuja, Nigeria.

Introduction: inspite of the demonstrable evidence of the preventive and protective ability of vaccines to reduce the outbreak of vaccine-preventable diseases, there are still some significant disease outbreaks recorded in our communities. In some settings, these outbreaks have been linked with poor vaccine management. Therefore, this study was conducted to compare the cold chain practices in Oyo State, Nigeria. Read More

View Article and Full-Text PDF

Nano-adjuvant based on silk fibroin for the delivery of recombinant hepatitis B surface antigen.

Biomater Sci 2021 Apr 19;9(7):2679-2695. Epub 2021 Feb 19.

Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.

Nanotechnology has a vital role in vaccine development. Nano-adjuvants, as robust delivery systems, could stimulate immune responses. Using nanoparticles (NPs) in vaccine formulations enhances the target delivery, immunogenicity, and stability of the antigens. Read More

View Article and Full-Text PDF

New GMP manufacturing processes to obtain thermostable HIV-1 gp41 virosomes under solid forms for various mucosal vaccination routes.

NPJ Vaccines 2020 May 18;5(1):41. Epub 2020 May 18.

Mymetics SA, 4 Route de la Corniche, 1066, Epalinges, Switzerland.

The main objective of the MACIVIVA European consortium was to develop new Good Manufacturing Practice pilot lines for manufacturing thermostable vaccines with stabilized antigens on influenza virosomes as enveloped virus-like particles. The HIV-1 gp41-derived antigens anchored in the virosome membrane, along with the adjuvant 3M-052 (TLR7/8 agonist) on the same particle, served as a candidate vaccine for the proof of concept for establishing manufacturing processes, which can be directly applied or adapted to other virosomal vaccines or lipid-based particles. Heat spray-dried powders suitable for nasal or oral delivery, and freeze-dried sublingual tablets were successfully developed as solid dosage forms for mucosal vaccination. Read More

View Article and Full-Text PDF

Immunomodulatory Role of NK Cells during Antiviral Antibody Therapy.

Vaccines (Basel) 2021 Feb 8;9(2). Epub 2021 Feb 8.

IGMM, Univ Montpellier, CNRS, Montpellier, France.

Monoclonal antibodies (mAbs) are now considered as a therapeutic approach to prevent and treat severe viral infections. Using a mouse retroviral model, we showed that mAbs induce protective immunity (vaccinal effects). Here, we investigated the role of natural killer (NK) cells on this effect. Read More

View Article and Full-Text PDF
February 2021

Grand Challenges in Pharmaceutical Research Series: Ridding the Cold Chain for Biologics.

Pharm Res 2021 Jan 8;38(1):3-7. Epub 2021 Feb 8.

Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, 20850, USA.

Biologics are complex pharmaceuticals that include formulated proteins, plasma products, vaccines, cell and gene therapy products, and biological tissues. These products are fragile and typically require cold chain for their delivery and storage. Delivering biologics, while maintaining the cold chain, whether standard (2°C to 8°C) or deepfreeze (as cold as -70°C), requires extensive infrastructure that is expensive to build and maintain. Read More

View Article and Full-Text PDF
January 2021

A lymph node-targeted Amphiphile vaccine induces potent cellular and humoral immunity to SARS-CoV-2.

Sci Adv 2021 02 5;7(6). Epub 2021 Feb 5.

Elicio Therapeutics, One Kendall Square, Suite 14303, Cambridge, MA 02139, USA.

The profound consequences of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mandate urgent development of effective vaccines. Here, we evaluated an Amphiphile (AMP) vaccine adjuvant, AMP-CpG, composed of diacyl lipid-modified CpG, admixed with the SARS-CoV-2 Spike-2 receptor binding domain protein as a candidate vaccine (ELI-005) in mice. AMP modification efficiently delivers CpG to lymph nodes, where innate and adaptive immune responses are generated. Read More

View Article and Full-Text PDF
February 2021

The Model of "Informed Refusal" for Vaccination: How to Fight against Anti-Vaccinationist Misinformation without Disregarding the Principle of Self-Determination.

Vaccines (Basel) 2021 Feb 1;9(2). Epub 2021 Feb 1.

Department of Anatomical, Histological, Forensic and Orthopaedic Sciences, Sapienza University of Rome, Viale Regina Elena 336, 00161 Rome, Italy.

Vaccines are arguably a public health success story as well as an incredibly cost-effective medical resource. Despite this, worldwide concerns about their safety are growing, with the risk of increased morbidity and mortality in vaccine-preventable diseases because of vaccine refusal. The global political trend in developed countries is to increasingly reduce mandates and the compulsory nature of vaccination programs. Read More

View Article and Full-Text PDF
February 2021

Potential Risks and Benefits of Multiple Sclerosis Immune Therapies in the COVID-19 Era: Clinical and Immunological Perspectives.

Neurotherapeutics 2021 01 2;18(1):244-251. Epub 2021 Feb 2.

Department of Neurology, Rutgers Robert Wood Johnson Medical School, 125 Paterson Street, Suite 65000, New Brunswick, NJ, 08901, USA.

Coronavirus SARS-CoV2 has emerged as one of the greatest infectious disease health challenges in a century. Patients with multiple sclerosis (MS) have a particular vulnerability to infections through their use of immunosuppressive disease-modifying therapies (DMTs). Specific DMTs pose particular risk based on their mechanisms of action (MOA). Read More

View Article and Full-Text PDF
January 2021