21,512 results match your criteria ultrastructure measured

Vascular permeability of skeletal muscle microvessels in rat arterial ligation model: in vivo analysis using two-photon laser scanning microscopy.

Am J Physiol Regul Integr Comp Physiol 2021 May 5. Epub 2021 May 5.

Department of Engineering Science, University of Electro-Communications, Chofu, Tokyo, Japangrid.266298.1.

Peripheral artery disease (PAD) in the lower limb compromises oxygen supply due to arterial occlusion. Ischemic skeletal muscle is accompanied by capillary structural deformation. Therefore, using novel microscopy techniques, we tested the hypothesis that endothelial cell swelling temporally and quantitatively corresponds to enhanced microvascular permeability. Read More

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Qiangji Jianli Decoction Alleviates Hydrogen Peroxide-Induced Mitochondrial Dysfunction via Regulating Mitochondrial Dynamics and Biogenesis in L6 Myoblasts.

Oxid Med Cell Longev 2021 13;2021:6660616. Epub 2021 Apr 13.

Institute of Pi-Wei, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.

Oxidative stress can cause the excessive generation of reactive oxygen species (ROS) and has various adverse effects on muscular mitochondria. Qiangji Jianli decoction (QJJLD) is an effective traditional Chinese medicine (TCM) that is widely applied to improve muscle weakness, and it has active constituents that prevent mitochondrial dysfunction. To investigate the protective mechanism of QJJLD against hydrogen peroxide- (HO-) mediated mitochondrial dysfunction in L6 myoblasts. Read More

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Curcumin Alleviates Chronic Pain and Improves Cognitive Impairment via Enhancing Hippocampal Neurogenesis in Sciatic Nerve Constriction Rats.

J Pain Res 2021 20;14:1061-1070. Epub 2021 Apr 20.

Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China.

Purpose: Cognitive impairment is a complication that most frequently happens in patients with chronic neuropathic pain and has limited effective therapy. The aim of this study was to explore the effects of curcumin on the cognitive deficit in rats with peripheral nerve injury induced-neuropathic pain.

Methods: The neuropathic pain rat model was constructed using chronic constriction injury (CCI). Read More

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The first record of Vairimorpha hostounsky sp. nov. Infection in the blue shieldbug, Zicrona caerulea Linnaeus, 1758 (Hemiptera: Pentatomidae).

Microb Pathog 2021 Apr 20;155:104883. Epub 2021 Apr 20.

Department of Plant and Animal Production, Espiye Vocational School, Giresun University, 28600, Giresun, Turkey. Electronic address:

A microsporidian pathogen was originally identified using molecular techniques from Oulema melanopus L. (Coleoptera, Chrysomelidae) under a "working name" Nosema oulemae, but its description was never published. In the present study, a microsporidian infection was detected in the Blue Shieldbug, Zicrona caerulea for the first time, showing 99. Read More

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Liraglutide Regulates the Kidney and Liver in Diabetic Nephropathy Rats through the miR-34a/SIRT1 Pathway.

J Diabetes Res 2021 5;2021:8873956. Epub 2021 Apr 5.

Department of Endocrinology, People's Hospital of Shenzhen Baoan District, The Second School of Clinical Medicine, Southern Medical University, The Second Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong, China.

Purpose: To explore the regulatory effects of liraglutide on the kidney and liver through the miR-34a/SIRT1 pathway with related factors in diabetic nephropathy (DN) rats.

Methods: DN rats were randomly divided into two groups ( = 10) and were injected with liraglutide or normal saline twice a day. The 24-hour urine microalbumin content and biochemical index levels were measured. Read More

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Energy metabolism disorder mediated ammonia gas-induced autophagy via AMPK/mTOR/ULK1-Beclin1 pathway in chicken livers.

Ecotoxicol Environ Saf 2021 Apr 11;217:112219. Epub 2021 Apr 11.

College of Animal Science and Technology, Northeast Agricultural University, NO. 600 Chang Jiang Road, Xiang Fang District, Harbin 150030, China. Electronic address:

Ammonia gas is a well-known environmental pollution gas, threatening human health. Ammonia gas is also one of the most harmful gases to livestock and poultry for many years. Many studies have demonstrated toxic effect of ammonia gas on animal health, such as eyes, respiratory system, and digestive system. Read More

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Mechanisms of microRNA‑142 in mitochondrial autophagy and hippocampal damage in a rat model of epilepsy.

Int J Mol Med 2021 Jun 13;47(6). Epub 2021 Apr 13.

Department of Neurology, The First Hospital of Changsha, Changsha, Hunan 410013, P.R. China.

Researchers have confirmed the microRNA (miRNA/miR)‑epilepsy association in rodent models of human epilepsy via a comprehensive database. However, the mechanisms of miR‑142 in epilepsy have not been extensively studied. In the present study, a rat model of epilepsy was first established by an injection of lithium chloride‑pilocarpine and the successful establishment of the model was verified via electroencephalogram monitoring. Read More

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Pendimethalin-based herbicide impairs cellular immune response and haemocyte morphology in a beneficial ground beetle.

J Insect Physiol 2021 Apr 6;131:104236. Epub 2021 Apr 6.

Department of Biology, Ecology and Earth Science, University of Calabria, Italy. Electronic address:

Herbicides have become the most commonly applied agrochemicals in agroecosystems. Thus, basic knowledge of their physiological effects on insects is needed, especially for understanding their impact on beneficial insect species. In this study, we evaluated the effect of a pendimethalin-based herbicide (PND) on the cellular immune response of the carabid beetle Harpalus (Pseudoophonus) rufipes (De Geer 1774) (Coleoptera, Carabidae), acting as biocontrol agent in agroecosystems. Read More

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Cyclovirobuxine D Induces Apoptosis and Mitochondrial Damage in Glioblastoma Cells Through ROS-Mediated Mitochondrial Translocation of Cofilin.

Front Oncol 2021 19;11:656184. Epub 2021 Mar 19.

Department of Pharmacy, Daping Hospital, Army Medical University, Chongqing, China.

Background: Cyclovirobuxine D (CVBD), a steroidal alkaloid, has multiple pharmacological activities, including anti-cancer activity. However, the anti-cancer effect of CVBD on glioblastoma (GBM) has seldom been investigated. This study explores the activity of CVBD in inducing apoptosis of GBM cells, and examines the related mechanism in depth. Read More

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Bioinspired Green Synthesis of Zinc Oxide Nanoparticles from a Native Strain RNT6: Characterization and Antibacterial Activity against Rice Panicle Blight Pathogens and .

Nanomaterials (Basel) 2021 Mar 30;11(4). Epub 2021 Mar 30.

State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.

and are seed-borne rice pathogens that cause bacterial panicle blight (BPB) disease, resulting in huge rice yield losses worldwide. However, the excessive use of chemical pesticides in agriculture has led to an increase in environmental toxicity. Microbe-mediated nanoparticles (NPs) have recently gained significant attention owing to their promising application in plant disease control. Read More

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New Approach to Antifungal Activity of Fluconazole Incorporated into the Porous 6-Anhydro-α-l-Galacto-β-d-Galactan Structures Modified with Nanohydroxyapatite for Chronic-Wound Treatments-In Vitro Evaluation.

Int J Mol Sci 2021 Mar 18;22(6). Epub 2021 Mar 18.

Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland.

New fluconazole-loaded, 6-Anhydro-α-l-Galacto-β-d-Galactan hydrogels incorporated with nanohydroxyapatite were prepared and their physicochemical features (XRD, X-ray Diffraction; SEM-EDS, Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy; ATR-FTIR, Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy), fluconazole release profiles and enzymatic degradation were determined. Antifungal activity of pure fluconazole was tested using species (, , ), species (, ) and species (, ) reference strains and clinical isolates. Standard microdilution method was applied, and fluconazole concentrations of 2-250 µg/mL were tested. Read More

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In vitro characterisation of low-cost synthetic meshes intended for hernia repair in the UK.

Hernia 2021 Apr 2. Epub 2021 Apr 2.

Centre for 3D Models of Health and Disease, Division of Surgery and Interventional Science, University College London, London, UK.

Purpose: Low-cost meshes (LCM) were repurposed for the repair of hernias in the developing world. In vivo studies have shown LCM to have comparable results to commercial meshes (CM) at a fraction of the cost. However, little has been done to characterise the mechanical and biocompatible properties of LCM, preventing its clinical use in the UK. Read More

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Sildenafil improves right ventricular remodelling in monocrotaline-induced rats by decreasing myocardial apoptosis and activating peroxisome proliferator-activated receptors.

J Pharm Pharmacol 2021 Mar;73(2):145-151

Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, China.

Objectives: To assess the effect of sildenafil on monocrotaline-induced right ventricular (RV) remodeling and investigate the possible mechanism.

Methods: Rats were subcutaneously injected with monocrotaline to establish an RV remodeling model and then administered sildenafil (25 mg/kg) from days 1 to 28. After 28 days of administration, the RV systolic pressure and the RV hypertrophy index (RVHI) were measured. Read More

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The Ultrastructure of the Nictitating Membrane of the Little Penguin (, Aves).

Integr Org Biol 2020 5;2(1):obaa048. Epub 2021 Jan 5.

Department of Optometry and Vision Science, University of New South Wales, Kensington, NSW 2052, Australia.

The ultrastructure of the nictitating membrane in the little penguin was studied using both scanning and transmission electron microscopy to improve our understanding of the function of ocular adnexa in diving birds. Following euthanasia, eyes were enucleated and immersion fixed in Karnovsky's fixative. The nictitating membrane and conjunctiva were embedded in araldite and semi- or ultra-thin sections were stained and photographed using compound and transmission electron microscopes, respectively. Read More

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January 2021

[Effects of mild moxibustion on Beclin-1/Bcl-2 expression in spinal cord of rats with cervical spondylotic radiculopathy].

Zhen Ci Yan Jiu 2020 Oct;45(10):799-805

The First Clinical Medical College, Guangxi University of Chinese Medicine, Nanning 530001, China.

Objective: To observe the effects of mild moxibustion on the expression of autophagy and apoptosis factors Beclin-1, Bcl-2 mRNA and protein in spinal cord (including nerve root tissues) of cervical spondylotic radiculopathy (CSR) rats, so as to explore the analgesic mechanism of mild moxibustion at "Dazhui" (GV14) on CSR.

Methods: SD rats were randomly divided into blank group, model group, mild moxibustion group and mild moxibustion+3-methyladenine(3-MA) group, with 10 rats in each group. CSR model was established by inserting the wire into the cervical nerve root. Read More

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October 2020

[Effect of moxibustion on expression of autophagy related molecules in synovial tissues of rheumatoid arthritis rats].

Zhen Ci Yan Jiu 2020 Oct;45(10):793-8

Institute of Acupuncture and Meridian, Anhui Academy of Traditional Chinese Medicine, Hefei 230038.

Objective: To observe the effect of moxibustion on expression of autophagy related gene(Atg), serine/threonine protein kinase-uncoordinated 51 like kinase-1 (ULK1), Beclin1 and microtubule associated proteins light chain 3 (LC3) and ultrastructure of synovium in rheumatoid arthritis (RA) rats, so as to explore its mechanisms underlying improvement of RA.

Methods: Forty SD rats were randomly divided into normal control, RA model, moxibustion, cigarette-roasting and medication groups (=8 rats in each group). The RA model was established by keeping the rats in wind, cold and wet environment for 12 h, once a day for 20 days and subcutaneous injection of Freund's adjuvant complete into the sole of the left hind paw. Read More

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October 2020

Doxorubicin (DOX) Gadolinium-Gold-Complex: A New Way to Tune Hybrid Nanorods as Theranostic Agent.

Int J Nanomedicine 2021 17;16:2219-2236. Epub 2021 Mar 17.

CNRS, UMR 7244, CSPBAT, Laboratoire de Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques, Université Sorbonne Paris Nord, Bobigny, 93000, France.

Introduction: In this paper, we have designed and formulated, a novel synthesis of doxorubicin (DOX) loaded bimetallic gold nanorods in which gold salt (HAuCl) is chelated with anthracycline (DOX), diacid polyethylene-glycol (PEG-COOH) and gadolinium salt (GdCl * 6 HO) to form DOX IN-Gd-AuNRs compared with DOX ON-Gd-AuNRs in which the drug was grafted onto the bimetallic pegylated nanoparticle surface by electrostatic adsorption.

Material And Method: The physical and chemical evaluation was performed by spectroscopic analytical techniques (Raman spectroscopy, UV-Visible and transmission electron microscopy (TEM)). Magnetic features at 7T were also measured. Read More

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Effect of ketamine on mood dysfunction and spatial cognition deficits in PTSD mouse models via HCN1-BDNF signaling.

J Affect Disord 2021 05 4;286:248-258. Epub 2021 Mar 4.

School of Psychology, Weifang Medical University, 7166# Baotong West Street, Weifang, Shandong 261053, China. Electronic address:

Background: Post-traumatic stress disorder (PTSD) is a debilitating mental disease with high morbidity and major social and economic relevance. No efficient treatment for PTSD has thus far been identified. Clinical research has shown that ketamine can rapidly alleviate symptoms in patients with chronic PTSD; however, its pharmacological mechanism has yet to be determined. Read More

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Quercetin Attenuates Diabetic Peripheral Neuropathy by Correcting Mitochondrial Abnormality via Activation of AMPK/PGC-1α Pathway and .

Front Neurosci 2021 3;15:636172. Epub 2021 Mar 3.

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

The AMPK/PGC-1α pathway-mediated mitochondrial dysfunction has been supposed to play a crucial role in pathogenesis of diabetic peripheral neuropathy (DPN). The present study investigated the neuroprotective potential of quercetin, a natural AMPK activator. Streptozotocin (STZ)-induced diabetic rats that developed DPN phenotype were orally administrated with quercetin (30 and 60 mg/kg per day) for 6 weeks. Read More

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Evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells.

Sci Rep 2021 Mar 18;11(1):6250. Epub 2021 Mar 18.

Department of Ophthalmology, Dongguk University, Ilsan Hospital, 814, Siksadong, Ilsan-dong-gu, Goyang, Gyunggido, 410-773, South Korea.

Moxifloxacin hydrochloride (MXF) is widely used for the prevention of bacterial endophthalmitis after intraocular surgeries. However, the safety issue of intracameral injection of MXF for human corneal endothelial cells (HCECs) is still debatable. In this study, we investigated concentration-dependent cytotoxicity (0. Read More

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Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles.

J Vis Exp 2021 02 27(168). Epub 2021 Feb 27.

Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science, LVTS, INSERM, UMR 1148; Services de Biochimie et Médecine Nucléaire, Hôpital Avicenne Assistance Publique-Hôpitaux de Paris;

Magnetic nanoparticles, made of iron oxide, present a peculiar interest for a wide range of biomedical applications for which they are often internalized in cells and then left within. One challenge is to assess their fate in the intracellular environment with reliable and precise methodologies. Herein, we introduce the use of the vibrating sample magnetometer (VSM) to precisely quantify the integrity of magnetic nanoparticles within cells by measuring their magnetic moment. Read More

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February 2021

Characterisation of selenium and tellurium nanoparticles produced by Aureobasidium pullulans using a multi-method approach.

J Chromatogr A 2021 Apr 27;1642:462022. Epub 2021 Feb 27.

Trace Element Speciation Laboratories, Dept. of Chemistry, University of Aberdeen, AB24 3UE, United Kingdom; Institute of Chemistry, Environmental Analytical Chemistry, University of Graz, 8010 Graz, Austria. Electronic address:

Aureobasidium pullulans was grown in liquid culture media amended with selenite and tellurite and selenium (Se) and tellurium (Te) nanoparticles (NPs) were recovered after 30 d incubation. A separation method was applied to recover and characterise Se and Te NPs by asymmetric flow field flow fractionation (AF4) with online coupling to multi-angle light scattering (MALS), ultraviolet visible spectroscopy (UV-Vis), and inductively coupled plasma mass spectrometry (ICP-MS) detectors. Additional characterisation data was obtained from transmission electron microscopy (TEM), and dynamic light scattering (DLS). Read More

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Characteristic Light and Electron Microscopic Features of Adelina melolonthae, a Coccidian Pathogen of the European Cockchafer, Melolontha melolontha (Coleoptera/Scarabaeidae).

Acta Parasitol 2021 Mar 13. Epub 2021 Mar 13.

Institute of Biology/Zoology, Freie Universität Berlin, Königin-Luise-Str. 1-3, 14195, Berlin, Germany.

Purpose: The aim of this study was to investigate and document the characteristic electron microscopic features of the oocyst of Adelina melolonthae, a coccidian pathogen of the European cockchafer, Melolontha melolontha (Coleoptera: Scarabaeidae).

Methods: Larvae of M. melolontha were collected at Ordu, Turkey. Read More

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Mesenchymal Stem Cell-Derived Extracellular Vesicle Isolation and Their Protein Cargo Characterization.

Methods Mol Biol 2021 ;2259:3-12

Grupo de Terapia Celular y Medicina Regenerativa. Dpto. de Fisioterapia, Medicina y Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidade da Coruña, INIBIC-CHUAC, Agrupación estratégica CICA-INIBIC, A Coruña, Spain.

In the present protocol, extracellular vesicles (EVs) released from a primary culture of human umbilical cord mesenchymal stem cells (MSCs) were isolated by ultracentrifugation processes, characterized by transmission electron microscopy (TEM) and measured by nanoparticle tracking analysis (NTA). Protein was extracted from EVs using RIPA buffer and then was assessed for integrity. The proteomic content of the total EV protein samples was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) after labeling by tandem mass tag (TMT). Read More

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Dual Functional Eudragit S100/L30D-55 and PLGA Colon-Targeted Nanoparticles of Iridoid Glycoside for Improved Treatment of Induced Ulcerative Colitis.

Int J Nanomedicine 2021 24;16:1405-1422. Epub 2021 Feb 24.

Food Science College, Northeast Agricultural University, Harbin, People's Republic of China.

Aim: Iridoid glycosides (IG) as the major active fraction of Lindl. has a proven anti-inflammatory effect for ulcerative colitis (UC). However, its current commercial formulations are hampered by low bioavailability and unable to reach inflamed colon. Read More

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Interleukin-1β-Treated Mesenchymal Stem Cells Inhibit Inflammation in Hippocampal Astrocytes Through Exosome-Activated Nrf-2 Signaling.

Int J Nanomedicine 2021 22;16:1423-1434. Epub 2021 Feb 22.

Hanan Branch of Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, 150001, People's Republic of China.

Background: Interleukin-1β (IL-1)-treated mesenchymal stem cells (MSCs) and IL-1-MSCs-conditioned medium (CM) exert anti-inflammatory roles. Astrocytes are essential for the modulation of synaptic activity and neuronal homeostasis in the brain. Exosomes are the critical mediators in intercellular communication. Read More

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3D Metal-Organic Frameworks Based on Co(II) and Bithiophendicarboxylate: Synthesis, Crystal Structures, Gas Adsorption, and Magnetic Properties.

Molecules 2021 Feb 26;26(5). Epub 2021 Feb 26.

Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.

Three new 3D metal-organic porous frameworks based on Co(II) and 2,2'-bithiophen-5,5'-dicarboxylate (btdc) [Co(btdc)(bpy)]·4DMF, ; [Co(btdc)(pz)(dmf)]·4DMF·1.5HO, ; [Co(btdc)(dmf)]∙2DMF∙2HO, (bpy = 2,2'-bipyridyl, pz = pyrazine, dmf = ,-dimethylformamide) were synthesized and structurally characterized. All compounds share the same trinuclear carboxylate building units {Co(RCOO)}, connected either by btdc ligands (, ) or by both btdc and pz bridging ligands (). Read More

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February 2021

Characterization of the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds by SR-FTIR.

BMC Musculoskelet Disord 2021 Mar 1;22(1):235. Epub 2021 Mar 1.

Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Background: Bone-tendon interface (enthesis) plays a pivotal role in relaxing load transfer between otherwise structurally and functionally distinct tissue types. Currently, decellularized extracellular matrix (DEM) from enthesis provide a natural three-dimensional scaffold with tissue-specific orientations of extracellular matrix molecules for enthesis regeneration, however, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff by SR-FTIR have not been reported.

Methods: Native enthesis tissues (NET) harvested from rabbit rotator cuff were sectioned into cuboid (about 30 mm × 1. Read More

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Pilot study on the effects of preservatives on corneal collagen parameters measured by small angle X-ray scattering analysis.

BMC Res Notes 2021 Feb 27;14(1):78. Epub 2021 Feb 27.

Department of Clinical Sciences, Ross University of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.

Objective: Small angle X-ray scattering (SAXS) analysis is a sensitive way of determining the ultrastructure of collagen in tissues. Little is known about how parameters measured by SAXS are affected by preservatives commonly used to prevent autolysis. We determined the effects of formalin, glutaraldehyde, Triton X and saline on measurements of fibril diameter, fibril diameter distribution, and D-spacing of corneal collagen using SAXS analysis. Read More

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February 2021

Antioxidant and Hepatoprotective Effects of Acidic-Hydrolysis Residue Polysaccharides from Shiitake Culinary-Medicinal Mushroom Lentinus edodes (Agaricomycetes) in Mice.

Int J Med Mushrooms 2021 ;23(2):85-96

College of Life Sciences, Shandong Agricultural University, Tai'an 271018, People's Republic of China.

The present work was designed to investigate the antioxidant and hepatoprotective effects of acidic-hydrolysis Lentinus edodes residue polysaccharides (Ac-LRP) on lipopolysaccharide-induced liver injury in mice. Hepatoprotective effects were observed in liver treated with Ac-LRP at doses of 200, 400, and 600 mg/kg body weight, respectively. Activities of superoxide dismutase, glutathione peroxide, catalase, total antioxidant capacity, alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase and levels of total cholesterol, triglyceride, malondialdehyde, and lipid peroxidation in liver or serum samples were analyzed. Read More

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January 2021