Cryobiology 2020 Nov 27. Epub 2020 Nov 27.
Dipartimento di Ingegneria Meccanica, Chimica e Dei Materiali, Facoltà di Ingegneria e Architettura, Università Degli Studi di Cagliari, Via Marengo 2, 09123, Cagliari, Italy. Electronic address:
Recently, a mathematical model able to describe the non-perfect osmotic behavior of cells during cryopreservation was proposed. The model improves the two-parameter formalism typically adopted in cryopreservation literature by allowing the transmembrane permeation of ions/salt, through the temporary opening of mechanosensitive channels whenever membrane stretching occurs: cells can reach an equilibrium volume different from the initial one, when isotonic conditions are re-established after contacting with impermeant or permeant solutes, such as sucrose or a cryoprotectant agent like dimethyl sulfoxide, respectively. Although the model was conceived as a conservative development of the two-parameter formalism to avoid over-parameterization, a complex picture of the system emerges. Read More