35,842 results match your criteria types mrna

CD4+ and CD25+ T cell response to short-time interferon-beta therapy on IL10, IL23A and FOXP3 genes in multiple sclerosis patients.

Int J Clin Pract 2021 Apr 22:e14238. Epub 2021 Apr 22.

Department of Medical Biology, Faculty of Medicine, Yeditepe University, 34755, Istanbul, Turkey.

Aim Of The Study: Interferon-beta (IFN-β), multiple sclerosis (MS) drug for years, does not have therapeutic effects on each patient. Yet, a considerable portion has experienced no therapeutic response to IFN-β. Therefore, it is necessary to determine disease-specific biomarkers that affect drug response. Read More

View Article and Full-Text PDF

Tissue-type specific accumulation of the plastoglobular proteome, transcriptional networks and plastoglobular functions.

J Exp Bot 2021 Apr 22. Epub 2021 Apr 22.

School of Integrative Plant Sciences (SIPS), Section of Plant Biology, Cornell University, Ithaca, New York 14853, USA.

Plastoglobules (PGs) are dynamic protein-lipid micro-compartments in plastids enriched for isoprenoid-derived metabolites. Chloroplast PGs support formation, remodeling and controlled dismantling of thylakoids during developmental transitions and environmental responses. However, the specific molecular functions of most PG proteins are still poorly understood. Read More

View Article and Full-Text PDF

Single-cell RNA sequencing of mouse islets exposed to proinflammatory cytokines.

Life Sci Alliance 2021 Jun 21;4(6). Epub 2021 Apr 21.

Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA

Exposure to proinflammatory cytokines is believed to contribute to pancreatic β-cell damage during diabetes development. Although some cytokine-mediated changes in islet gene expression are known, the heterogeneity of the response is not well-understood. After 6-h treatment with IL-1β and IFN-γ alone or together, mouse islets were subjected to single-cell RNA sequencing. Read More

View Article and Full-Text PDF

[New Hopes in Vaccine Technology: mRNA Vaccines].

Engin Yılmaz

Mikrobiyol Bul 2021 Apr;55(2):265-284

Hacettepe University Faculty of Medicine, Department of Medical Biology, Emeritus, Ankara, Turkey.

The major disadvantages of traditional virus vaccines are time constraints in development and difficulties in large-scale production. Therefore, there is a need to develop stronger and more versatile vaccine platforms. mRNA vaccines constitute a promising alternative to traditional vaccine approaches due to their high potential, rapid development capacity, low cost production and safe administration potential. Read More

View Article and Full-Text PDF

Peptides and Antibiotic Therapy: Advances in Design and Delivery.

Acc Chem Res 2021 Apr 21. Epub 2021 Apr 21.

Division of Pulmonary, Allergy and Immunology, Cystic Fibrosis, and Sleep, Department of Pediatrics and Center for Cystic Fibrosis and Airway Diseases Research, Children's Healthcare of Atlanta, Emory University School of Medicine, 1510 Clifton Road NE, Suite 3009, Atlanta, Georgia 30322, United States.

ConspectusAntibiotic resistance (AMR) is an increasing public health crisis worldwide. This threatens our ability to adequately care for patients with infections due to multi-drug-resistant (MDR) pathogens. As such, there is an urgent need to develop new classes of antimicrobials that are not based on currently utilized antibiotic scaffolds. Read More

View Article and Full-Text PDF

Transdermal Delivery of Adipocyte Phospholipase A2 siRNA using Microneedles to Treat Thyroid Associated Ophthalmopathy-Related Proptosis.

Cell Transplant 2021 Jan-Dec;30:9636897211010633

Department of Obstetrics & Gynaecology, Faculty of Medicine, 26451The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.

Thyroid associated ophthalmopathy (TAO) is an organ-specific autoimmune disease occurring in patients with thyroid disease. Patients with TAO-related proptosis is largely due to excessive orbital adipose tissue Adipocyte phospholipase A2 (AdPLA) is one of the most important regulatory factors in adipocyte lipolysis, which may be associated with TAO-related proptosis. Thus, silencing AdPLA by RNA interference may be beneficial for the treatment of TAO. Read More

View Article and Full-Text PDF

Musk ketone induces apoptosis of gastric cancer cells via downregulation of sorbin and SH3 domain containing 2.

Mol Med Rep 2021 Jun 21;23(6). Epub 2021 Apr 21.

Department of Basic Medical Sciences, Qinghai University Medical College, Xining, Qinghai 810016, P.R. China.

Musk ketone exerts antiproliferative effects on several types of cancer, such as lung and breast cancer. However, the effects and underlying mechanisms of action of musk ketone in gastric cancer (GC) are poorly understood. The present study aimed to investigate the effects of musk ketone in GC cells. Read More

View Article and Full-Text PDF

Variation of microRNA expression in the human placenta driven by population identity and sex of the newborn.

BMC Genomics 2021 Apr 20;22(1):286. Epub 2021 Apr 20.

Skolkovo Institute of Science and Technology, 121205, Moscow, Russia.

Background: Analysis of lymphocyte cell lines revealed substantial differences in the expression of mRNA and microRNA (miRNA) among human populations. The extent of such population-associated differences in actual human tissues remains largely unexplored. The placenta is one of the few solid human tissues that can be collected in substantial numbers in a controlled manner, enabling quantitative analysis of transient biomolecules such as RNA transcripts. Read More

View Article and Full-Text PDF

COVID-19 Vaccine Concerns: Fact or Fiction?

Antoine Barbari

Exp Clin Transplant 2021 Mar 31. Epub 2021 Mar 31.

From the Rafik Hariri University Hospital, Bir Hassan, Beirut, Lebanon.

One year has elapsed since a team of Chinese scientists reported the first case of COVID-19 in Wuhan, China on January 8, 2020, after sequencing the first viral genetic material. Since then, many vaccines were rushed into testing, bypassing animal experimentations, with more than 200 pharma companies in different countries declaring the development of different vaccines, each with their own strategy for generating immunity, despite the arguments of many infectious disease experts that 18 months for a first vaccine is an incredibly aggressive schedule because it takes an average of 10 years to develop a vaccine. Ten vaccine candidates have already entered phase 3 clinical trials in humans. Read More

View Article and Full-Text PDF

4E-BP2-dependent translation in parvalbumin neurons controls epileptic seizure threshold.

Proc Natl Acad Sci U S A 2021 Apr;118(15)

Department of Anesthesia and Faculty of Dentistry, McGill University, Montreal, QC H3A 2B4, Canada;

The mechanistic/mammalian target of rapamycin complex 1 (mTORC1) integrates multiple signals to regulate critical cellular processes such as mRNA translation, lipid biogenesis, and autophagy. Germline and somatic mutations in mTOR and genes upstream of mTORC1, such as , , , , and components of GATOR1 and KICSTOR complexes, are associated with various epileptic disorders. Increased mTORC1 activity is linked to the pathophysiology of epilepsy in both humans and animal models, and mTORC1 inhibition suppresses epileptogenesis in humans with tuberous sclerosis and animal models with elevated mTORC1 activity. Read More

View Article and Full-Text PDF

Early Safety Monitoring of COVID-19 Vaccines in Healthcare Workers.

J Korean Med Sci 2021 Apr 19;36(15):e110. Epub 2021 Apr 19.

Department of Hospital Infection Control, Korea University Anam Hospital, Seoul, Korea.

Hospital-based surveillance for adverse events was conducted on healthcare workers after they received the first dose of coronavirus disease 2019 (COVID-19) vaccine. Among the two new platform vaccines (messenger RNA- and adenoviral vector-based vaccines), the rates of systemic adverse events were significantly higher among adenovirus-vectored vaccine recipients. Fatigue (87. Read More

View Article and Full-Text PDF

The impact of damage-associated molecules released from canine tumor cells on gene expression in macrophages.

Sci Rep 2021 Apr 19;11(1):8525. Epub 2021 Apr 19.

Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.

Dying or damaged cells that are not completely eradicated by the immune system release their intracellular components in the extracellular space. Aberrant exposure of the damage-associated molecules to the immune system is often associated with inflammation and cancer pathogenesis. Thus, elucidating the role of damage-associated molecules in inducing sterile immune responses is crucial. Read More

View Article and Full-Text PDF

PTEN loss correlates with T cell exclusion across human cancers.

BMC Cancer 2021 Apr 19;21(1):429. Epub 2021 Apr 19.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Background: Recent evidences had shown that loss in phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was associated with immunotherapy resistance, which may be attributed to the non-T-cell-inflamed tumor microenvironment. The impact of PTEN loss on tumor microenvironment, especially regarding T cell infiltration across tumor types is not well understood.

Methods: Utilizing The Cancer Genome Atlas (TCGA) and publicly available dataset of immunotherapy, we explored the correlation of PTEN expressing level or genomic loss with tumor immune microenvironment and response to immunotherapy. Read More

View Article and Full-Text PDF

The Gene Family Serves as a Prognostic Biomarker in Clear Cell Renal Cell Carcinoma.

Front Oncol 2021 31;11:620126. Epub 2021 Mar 31.

The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.

The cysteine-serine-rich nuclear protein () family has prognostic value for various cancers. However, the association between this proteins and prognosis of clear cell renal cell carcinoma (ccRCC) remains unclear. This study aimed to determine the prognostic value of the family for patients with ccRCC. Read More

View Article and Full-Text PDF

Expression profiling of CPS1 in Correa's cascade and its association with gastric cancer prognosis.

Oncol Lett 2021 Jun 2;21(6):441. Epub 2021 Apr 2.

Clinical Research Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201821, P.R. China.

Carbamoyl phosphate synthetase 1 (CPS1), which is the antigen for the hepatocyte paraffin 1 antibody, exhibits focal immunoreactivity in adenocarcinoma from the gastrointestinal tract, but its expression profiles and roles in gastric cancer (GC) remain largely unknown. The present study aimed to determine the expression pattern and prognostic value of CPS1 in Correa's cascade using tissues from 32 patients with chronic atrophic gastritis with intestinal metaplasia (IM), 62 patients with low- or high-grade intraepithelial neoplasia (IN) and 401 patients with GC. The expression of CPS1 was diffuse and strongly positive in 32 cases (100%) of IM of the glandular epithelium, and gradually downregulated in Correa's cascade, with a strongly positive ratio of 21 (70%) in low-grade IN and 4 (12. Read More

View Article and Full-Text PDF

Aquaporin 1 knockdown inhibits triple-negative breast cancer cell proliferation and invasion and .

Oncol Lett 2021 Jun 1;21(6):437. Epub 2021 Apr 1.

Department of Breast Surgery, Guangxi Medical University Cancer Hospital, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.

Aquaporin 1 (AQP1) contributes to the progression of several cancer types, but its potential involvement in triple-negative breast cancer (TNBC) is unclear. The aim of the present study was to examine the role of AQP1 in cell proliferation and invasion in TNBC. Reverse transcription-quantitative PCR analysis and western blotting were used to detect AQP1 expression in different cell lines. Read More

View Article and Full-Text PDF

Effects of a combined therapy of bortezomib and ionizing radiation on chondrosarcoma three-dimensional spheroid cultures.

Oncol Lett 2021 Jun 30;21(6):428. Epub 2021 Mar 30.

Department of Radiation Oncology, Medical University of Vienna, A-1090 Vienna, Austria.

Chondrosarcomas represent a heterogeneous group of primary bone cancers that are characterized by hyaline cartilaginous neoplastic tissue and are predominantly resistant to radiation and chemotherapy. However, adjuvant radiotherapy is often recommended in inoperable cases or after incomplete resections. To improve the efficiency of treatment, the present study tested a combination therapy with ionizing radiation (IR) and the proteasome inhibitor bortezomib. Read More

View Article and Full-Text PDF

RRM2B Is Frequently Amplified Across Multiple Tumor Types: Implications for DNA Repair, Cellular Survival, and Cancer Therapy.

Front Genet 2021 12;12:628758. Epub 2021 Mar 12.

Cancer Prevention and Control Program, Fox Chase Cancer Center, Philadelphia, PA, United States.

plays a crucial role in DNA replication, repair and oxidative stress. While germline mutations have been implicated in mitochondrial disorders, its relevance to cancer has not been established. Here, using TCGA studies, we investigated alterations in cancer. Read More

View Article and Full-Text PDF

A 4-gene-based hypoxia signature is associated with tumor immune microenvironment and predicts the prognosis of pancreatic cancer patients.

World J Surg Oncol 2021 Apr 17;19(1):123. Epub 2021 Apr 17.

Department of General Surgery, Chaohu Hospital of Anhui Medical University, Chaohu, 238000, Anhui, China.

Background: Pancreatic cancer (PAC) is one of the most devastating cancer types with an extremely poor prognosis, characterized by a hypoxic microenvironment and resistance to most therapeutic drugs. Hypoxia has been found to be one of the factors contributing to chemoresistance in PAC, but also a major driver of the formation of the tumor immunosuppressive microenvironment. However, the method to identify the degree of hypoxia in the tumor microenvironment (TME) is incompletely understood. Read More

View Article and Full-Text PDF

MiR-25 and KLF4 relationship has early prognostic significance in the development of cervical cancer.

Pathol Res Pract 2021 Apr 9;222:153435. Epub 2021 Apr 9.

Ankara University, Biotechnology Institute, Ankara, Turkey. Electronic address:

Cervical squamous cell carcinoma (SCC) is one of the common cancer types among women. MicroRNAs (miRNAs) are small non-coding RNAs that play an important role in the formation and development of many cancer types by regulating expression of their targets. While many studies have investigated the relationship between miRNAs and cervical cancer, no robust miRNA biomarkers have been defined yet for diagnosis of cervical lesions. Read More

View Article and Full-Text PDF

Construction of miRNA-mRNA Regulatory Network and Prognostic Signature in Endometrial Cancer.

Onco Targets Ther 2021 6;14:2363-2378. Epub 2021 Apr 6.

Department of Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, People's Republic of China.

Introduction: This bioinformatic study confirmed a new miRNA-mRNA regulatory network and a prognostic signature in endometrial cancer (EC).

Materials And Methods: We downloaded RNA-seq and miRNA-seq data of EC from the TCGA database, then used EdegR package to screen differentially expressed miRNAs and mRNAs (DE-miRNAs and DE-mRNAs). Then, we constructed a regulatory network of EC-associated miRNAs and hub genes by Cytoscape, and determined the expression of unexplored miRNAs in EC tissues and normal adjacent tissues by quantitative Real-Time PCR (qRT-PCR). Read More

View Article and Full-Text PDF

miRGTF-net: Integrative miRNA-gene-TF network analysis reveals key drivers of breast cancer recurrence.

PLoS One 2021 14;16(4):e0249424. Epub 2021 Apr 14.

Faculty of Biology and Biotechnology, HSE University, Moscow, Russia.

Analysis of regulatory networks is a powerful framework for identification and quantification of intracellular interactions. We introduce miRGTF-net, a novel tool for construction of miRNA-gene-TF networks. We consider multiple transcriptional and post-transcriptional interaction types, including regulation of gene and miRNA expression by transcription factors, gene silencing by miRNAs, and co-expression of host genes with their intronic miRNAs. Read More

View Article and Full-Text PDF

Analysis of long non-coding RNAs in skeletal muscle of Bama Xiang pigs in response to heat stress.

Trop Anim Health Prod 2021 Apr 14;53(2):259. Epub 2021 Apr 14.

College of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China.

The aim of this study was to identify differentially expressed long non-coding RNAs (lncRNAs) molecules and predict their target genes related to muscle development and lipid metabolism in longissimus dorsi (LD) muscles of Bama Xiang pigs under constant heat stress. Ten male Bama Xiang pigs with an average initial body weight of 14 kg were randomly divided into control group (22°C) and heat stress (35 °C) group. The experiment lasted for 28 days. Read More

View Article and Full-Text PDF

Long non-coding RNA ADAMTS9-AS2 inhibits liver cancer cell proliferation, migration and invasion.

Exp Ther Med 2021 Jun 26;21(6):559. Epub 2021 Mar 26.

The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Long non-coding RNA (lncRNA) ADAM metallopeptidase with thrombospondin type 1 motif 9 antisense RNA 2 (ADAMTS9-AS2) is involved in various types of cancer, such as ovarian cancer, lung cancer and clear cell renal cell carcinoma. However, the roles of ADAMTS9-AS2 in liver cancer are not completely understood. The present study aimed to determine the functional role of ADAMTS9-AS2 in human liver cancer and investigate the potential underlying molecular mechanisms. Read More

View Article and Full-Text PDF

A novel focal adhesion related gene signature for prognostic prediction in hepatocellular carcinoma.

Aging (Albany NY) 2021 Apr 13;13(7):10724-10748. Epub 2021 Apr 13.

Department of Gastroenterology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Hepatocellular carcinoma (HCC) is a highly heterogeneous disease. Reduced expression of focal adhesion is considered as an important prerequisite for tumor cell invasion and metastasis. However, the prognostic value of focal adhesion related genes in HCC remains to be further determined. Read More

View Article and Full-Text PDF

Specific PIWI-interacting RNAs (piRNAs) and related small noncoding RNAs are associated with ovarian aging in Ames dwarf (df/df) mice.

J Gerontol A Biol Sci Med Sci 2021 Apr 13. Epub 2021 Apr 13.

Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, United States.

The Ames dwarf (df/df) mouse is a well-established model for delayed aging. MicroRNAs (miRNAs), the most studied small noncoding RNAs (sncRNAs), may regulate ovarian aging to maintain a younger ovarian phenotype in df/df mice. In this study, we profile other types of ovarian sncRNAs, PIWI-interacting RNAs (piRNAs) and piRNA-Like RNAs (piLRNAs) in young and aged df/df and normal mice. Read More

View Article and Full-Text PDF

The role and potential application of extracellular vesicles in liver cancer.

Sci China Life Sci 2021 Apr 9. Epub 2021 Apr 9.

Cancer Research Center, The First Affiliated Hospital, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, China.

Liver cancer is one of the most common causes of cancer-related death worldwide and mainly includes hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA). Extracellular vesicles (EVs) are membrane-derived nanometer-sized vesicles that can be released by different cell types under normal and pathological conditions and thus play important roles in the transmission of biological information between cells. Increasing evidence suggests that liver cancer cell-derived EVs may help establish a favorable microenvironment to support the proliferation, invasion and metastasis of cancer cells. Read More

View Article and Full-Text PDF

MicroRNA‑485‑5p suppresses the progression of esophageal squamous cell carcinoma by targeting flotillin‑1 and inhibits the epithelial‑mesenchymal transition.

Oncol Rep 2021 Jun 13;45(6). Epub 2021 Apr 13.

Research Centre, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.

As esophageal squamous cell carcinoma (ESCC) is one of the most frequently diagnosed cancers in Asia, it is crucial to uncover its underlying molecular mechanisms that support its development and progression. Several articles have reported that microRNA (miR)‑485‑5p inhibits the malignant phenotype in a number of cancer types, such as lung, gastric and breast cancer, but to the best of our knowledge, its function in ESCC has not been studied in depth until the present study. It is of great significance to probe the regulatory action and underlying mechanism of miR‑485‑5p in ESCC. Read More

View Article and Full-Text PDF

Upregulation of antioxidant and autophagy pathways via NRF2 activation protects spinal cord neurons from ozone damage.

Mol Med Rep 2021 Jun 13;23(6). Epub 2021 Apr 13.

Department of Pain Management, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, P.R. China.

Ozone therapy can relieve multiple types of pain but exhibits potential neurotoxicity, the mechanism of which is unclear. The present study aimed to identify the role of nuclear factor (erythroid‑derived‑2)‑related 2 (NRF2) in preventing spinal cord injury caused by ozone overdose. Primary neuronal cells were extracted from newborn Wistar rats and authenticated by immunofluorescence using anti‑microtubule‑associated protein 2 as a cell type‑specific marker. Read More

View Article and Full-Text PDF

Eotaxin-1/CCL11 is involved in cell migration in rheumatoid arthritis.

Sci Rep 2021 Apr 12;11(1):7937. Epub 2021 Apr 12.

Division of Rheumatology, Department of Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, Japan.

Eotaxin-1 (CCL11) induces the migration of different leukocyte types by interacting with CCR3. In rheumatoid arthritis (RA), fibroblast-like synoviocytes (FLS) are pathogenic effectors and a major CCR3-expressing cell. The aim of this study was to investigate the expression and function of CCL11 in RA FLS. Read More

View Article and Full-Text PDF