5,910 results match your criteria turnaround timemethods


A Proof of Concept of the Role of TDM-Based Clinical Pharmacological Advices in Optimizing Antimicrobial Therapy on Real-Time in Different Paediatric Settings.

Front Pharmacol 2021 27;12:755075. Epub 2021 Sep 27.

Department of Medical and Surgical Sciences, Alma Mater Studiorum University of Bologna, Bologna, Italy.

Antimicrobial treatment is quite common among hospitalized children. The dynamic age-associated physiological variations coupled with the pathophysiological alterations caused by underlying illness and potential drug-drug interactions makes the implementation of appropriate antimicrobial dosing extremely challenging among paediatrics. Therapeutic drug monitoring (TDM) may represent a valuable tool for assisting clinicians in optimizing antimicrobial exposure. Read More

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September 2021

[Factors associated with underreporting of cases of multidrug-resistant tuberculosis in the state of Rio de Janeiro, Brazil: probabilistic database linkage].

Cad Saude Publica 2021 8;37(10):e00293920. Epub 2021 Oct 8.

Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil.

This study estimated the proportion of underreporting of multidrug-resistant tuberculosis (MDR-TB) and associated factors in the State of Rio de Janeiro, Brazil, as well as the proportion of deaths in this group. A retrospective cohort study was conducted using probabilistic database linkage. Cases with the results of the drug sensitivity test (DST) with MDR-TB pattern recorded in the Laboratory Environment Management System (GAL) from 2010 to 2017 were linked to cases reported to the Special TB Treatments System (SITETB). Read More

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October 2021

Routine, Cost-Effective SARS-CoV-2 Surveillance Testing Using Pooled Saliva Limits Viral Spread on a Residential College Campus.

Microbiol Spectr 2021 Oct 13:e0108921. Epub 2021 Oct 13.

Department of Biological Sciences, Olivet Nazarene Universitygrid.261375.4, Bourbonnais, Illinois, USA.

Routine testing for SARS-CoV-2 is rare for institutes of higher education due to prohibitive costs and supply chain delays. During spring 2021, we routinely tested all residential students 1 to 2 times per week using pooled, RNA-extraction-free, reverse transcription quantitative PCR (RT-qPCR) testing of saliva at a cost of $0.43/sample with same-day results. Read More

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October 2021

Impact of centrifugation time and pneumatic tube transport on plasma concentrations of direct oral anticoagulants.

Int J Lab Hematol 2021 Oct 12. Epub 2021 Oct 12.

Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.

Introduction: Rapid results are needed when plasma concentrations of direct oral anticoagulants (DOACs) are required in acute clinical settings. We evaluated the impact of centrifugation time and pneumatic tube transport on DOAC plasma concentrations with the overall aim of reducing turnaround time.

Methods: Blood samples were spiked with rivaroxaban, apixaban or dabigatran in a low and a high concentration prior to centrifugation for 25 minutes (3163 g) or 5 minutes (3000 g) (n = 20 for each DOAC). Read More

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October 2021

Detection of malignant melanoma in H&E-stained images using deep learning techniques.

Tissue Cell 2021 Sep 29;73:101659. Epub 2021 Sep 29.

Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB, Canada. Electronic address:

Histopathological images are widely used to diagnose diseases including skin cancer. As digital histopathological images are typically of very large size, in the order of several billion pixels, automated identification of all abnormal cell nuclei and their distribution within multiple tissue sections would assist rapid comprehensive diagnostic assessment. In this paper, we propose a deep learning-based technique to segment the melanoma regions in Hematoxylin and Eosin (H&E) stained histopathological images. Read More

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September 2021

Amplification of Femtograms of Bacterial DNA Within 3 h Using a Digital Microfluidics Platform for MinION Sequencing.

ACS Omega 2021 Oct 23;6(39):25642-25651. Epub 2021 Sep 23.

Department of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota 55905-0002, United States.

Whole genome sequencing is emerging as a promising tool for the untargeted detection of a broad range of microbial species for diagnosis and analysis. However, it is logistically challenging to perform the multistep process from sample preparation to DNA amplification to sequencing and analysis within a short turnaround time. To address this challenge, we developed a digital microfluidic device for rapid whole genome amplification of low-abundance bacterial DNA and compared results with conventional in-tube DNA amplification. Read More

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October 2021

Statistical Process Control Charts for Monitoring Next-Generation Sequencing and Bioinformatics Turnaround in Precision Medicine Initiatives.

Front Oncol 2021 24;11:736265. Epub 2021 Sep 24.

Cancer Science Institute of Singapore, National University of Singapore, Singapore, Singapore.

Purpose: Precision oncology, such as next generation sequencing (NGS) molecular analysis and bioinformatics are used to guide targeted therapies. The laboratory turnaround time (TAT) is a key performance indicator of laboratory performance. This study aims to formally apply statistical process control (SPC) methods such as CUSUM and EWMA to a precision medicine programme to analyze the learning curves of NGS and bioinformatics processes. Read More

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September 2021

Electronic Health Record Network Research in Infectious Diseases.

Clin Ther 2021 Oct 7. Epub 2021 Oct 7.

Northwestern University Feinberg School of Medicine, Chicago, Illinois; Mary Ann & J. Milburn Smith Child Health Outcomes, Research, and Evaluation Center, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, Illinois.

With the marked increases in electronic health record (EHR) use for providing clinical care, there have been parallel efforts to leverage EHR data for research. EHR repositories offer the promise of vast amounts of clinical data not easily captured with traditional research methods and facilitate clinical epidemiology and comparative effectiveness research, including analyses to identify patients at higher risk for complications or who are better candidates for treatment. These types of studies have been relatively slow to penetrate the field of infectious diseases, but the need for rapid turnaround during the COVID-19 global pandemic has accelerated the uptake. Read More

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October 2021

CCRK/CDK20 regulates ciliary retrograde protein trafficking via interacting with BROMI/TBC1D32.

PLoS One 2021 8;16(10):e0258497. Epub 2021 Oct 8.

Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan.

CCRK/CDK20 was reported to interact with BROMI/TBC1D32 and regulate ciliary Hedgehog signaling. In various organisms, mutations in the orthologs of CCRK and those of the kinase ICK/CILK1, which is phosphorylated by CCRK, are known to result in cilia elongation. Furthermore, we recently showed that ICK regulates retrograde ciliary protein trafficking and/or the turnaround event at the ciliary tips, and that its mutations result in the elimination of intraflagellar transport (IFT) proteins that have overaccumulated at the bulged ciliary tips as extracellular vesicles, in addition to cilia elongation. Read More

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October 2021

Performance of a convolutional neural network algorithm for tooth detection and numbering on periapical radiographs.

Dentomaxillofac Radiol 2021 Oct 8:20210246. Epub 2021 Oct 8.

Division of Oral & Maxillofacial Radiology, Department of Care Planning and Restorative Sciences, University of Mississippi Medical Center School of Dentistry, Jackson, Mississippi, USA.

Objectives: The present study aimed to evaluate the performance of a Faster Region-based Convolutional Neural Network (R-CNN) algorithm for tooth detection and numbering on periapical images.

Methods: The data sets of 1686 randomly selected periapical radiographs of patients were collected retrospectively. A pre-trained model (GoogLeNet Inception v3 CNN) was employed for pre-processing, and transfer learning techniques were applied for data set training. Read More

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October 2021

Advanced Point-of-Care Testing Technologies for Human Acute Respiratory Virus Detection.

Adv Mater 2021 Oct 8:e2103646. Epub 2021 Oct 8.

Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, Stanford School of Medicine, Palo Alto, CA, 94304, USA.

The ever-growing global threats to human life caused by the human acute respiratory virus (RV) infections have cost billions of lives, created a significant economic burden, and shaped society for centuries. The timely response to emerging RVs could save human lives and reduce the medical care burden. The development of RV detection technologies is essential for potentially preventing RV pandemic and epidemics. Read More

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October 2021

A low-resource digital infrastructure to streamline viewing and interpretation of radiographic images: A case study of Uganda's hospital-wide environment.

Health Informatics J 2021 Oct-Dec;27(4):14604582211043153

Makerere University School of Computing and Informatics Technology, Kampala, Uganda.

Picture Archiving and Communication Systems (PACS) are said to improve patient quality of care through timely access to radiological images by clinicians. However, they are costly to be considered for hospital wide environment in low income countries. Ordinary core i3 computer systems (PCs) can provide an affordable and faster alternative solution for PACS workstations. Read More

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October 2021

Pathology Residents as Testing Personnel in the Hematology Laboratory.

Arch Pathol Lab Med 2021 Oct 7. Epub 2021 Oct 7.

Department of Laboratory Medicine, R.J. Tomsich Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, Ohio.

Context.—: Clinical laboratories and the training of pathology residents are tightly regulated environments. Compliance with regulatory requirements must be addressed when developing entrustable professional activities (EPAs) for pathology residents. Read More

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October 2021

Natural spring water gargle and direct RT-PCR for the diagnosis of COVID-19 (COVID-SPRING study).

J Clin Virol 2021 Oct 2;144:104995. Epub 2021 Oct 2.

Département de Microbiologie, infectiologie et Immunologie, Université de Montréal, Montréal, H2 × 3E4, Canada; Service de maladies infectieuses, Département de Médecine, CIUSSS de l'Est-de-l'Île-de-Montreal, Montréal, Canada.

Background: Nasopharyngeal swab has long been considered the specimen of choice for the diagnosis of respiratory viral infections, including SARS-CoV-2 infection, but it suffers from several drawbacks: its discomfort limits screening acceptability, and it is vulnerable to shortages in both specialized materials and trained healthcare workers in the context of a pandemic.

Methods: We prospectively compared natural spring water gargle to combined oro-nasopharyngeal swab (ONPS) for the diagnosis of coronavirus disease 2019 (COVID-19) in paired clinical specimens (1005 ONPS and 1005 gargles) collected from 987 unique early symptomatic as well as asymptomatic individuals from the community.

Results: Using a direct RT-PCR method with the Allplex™ 2019-nCoV Assay (Seegene), the clinical sensitivity of the gargle was 95. Read More

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October 2021

Reduced turnaround times through multi-sectoral community collaboration during the first surge of SARS-CoV-2 and associated effect on patient care and hospital operations.

PLoS One 2021 7;16(10):e0257302. Epub 2021 Oct 7.

Pennington Biomedical Research Center, Baton Rouge, LA, United States of America.

Background: In March 2020, an influx of admissions in COVID-19 positive patients threatened to overwhelm healthcare facilities in East Baton Rouge Parish, Louisiana. Exacerbating this problem was an overall shortage of diagnostic testing capability at that time, resulting in a delay in time-to-result return. An improvement in diagnostic testing availability and timeliness was necessary to improve the allocation of resources and ultimate throughput of patients. Read More

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October 2021

Rapid and Sensitive Pathogen Detection by DNA Amplification Using Janus Particle-Enabled Rotational Diffusometry.

Anal Chem 2021 Oct 7. Epub 2021 Oct 7.

Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan.

Rapid and sensitive detection of infectious bacteria is in all-time high demand to prevent the further spread of the infection and allow early medical intervention. In this study, we use rotational diffusometry (RD), a natural phenomenon characterized by Janus particles, to detect pathogens like by performing amplification of specific genes. This biosensing method is used to measure the change in viscosity of the fluid in the presence and absence of DNA in the solution by capturing images of modified microbeads at 10 Hz by a CCD camera followed by cross-correlation algorithm analysis. Read More

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October 2021

CRISPR-based platform for rapid, sensitive and field-deployable detection of scale drop disease virus in Asian sea bass (Lates calcarifer).

J Fish Dis 2021 Oct 6. Epub 2021 Oct 6.

Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand.

Scale drop disease virus (SDDV) is a major pathogen of Asian sea bass that has emerged in many countries across the Asia Pacific since 1992 and carries the potential to cause drastic economic losses to the aquaculture sector. The lack of an approved vaccine for SDDV necessitates timely prevention as the first line of defence against the disease, but current diagnostic platforms still face challenges that render them incompatible with field applications, particularly in resource-limited settings. Here, we developed a novel detection platform for SDDV based on a CRISPR-Cas12a-based nucleic acid detection technology combined with recombinase polymerase amplification (RPA-Cas12a). Read More

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October 2021

Effects of Changing the Reporting System from Decentralized/Modality-Based to Centralized/Subspecialized Radiology on Radiologists, Radiologic Technicians and Referring Physicians of a Multi-Center Radiology Network.

J Belg Soc Radiol 2021 16;105(1):45. Epub 2021 Sep 16.

Kantonsspital Sankt Gallen, CH.

Objectives: To determine the effects of reorganizing a radiology institute from decentralized/modality-based to centralized/subspecialized radiology on radiologists, radiologic technicians, and referring physicians at a multi-center radiology network.

Material And Methods: In 2017/2018 our multi-center radiology network was changed from decentralized/modality-based to centralized/subspecialized reporting. A survey was conducted among radiologists, technicians and two groups of referring physicians (main hospital and non-main hospitals). Read More

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September 2021

Active droplet-array microfluidics-based chemiluminescence immunoassay for point-of-care detection of procalcitonin.

Biosens Bioelectron 2021 Sep 30;195:113684. Epub 2021 Sep 30.

School of Medicine, South China University of Technology, Guangzhou, 510006, China; Department of Laboratory Medicine, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, 510180, China; Guangdong Engineering Technology Research Center of Microfluidic Chip Medical Diagnosis, Guangzhou, 510180, China. Electronic address:

The application of conventional chemiluminescence immunoassay (CLIA) in resource-limited settings is limited due to the large apparatus footprint, cumbersome operation and maintenance process, and high consumption of reagents. To address this issue, we developed an active droplet-array (ADA) microfluidics-based CLIA system, which consists of a compact microchip analyzer and microfluidic chips with preloaded reagents. The microfluidic chip contains microslit-connected microchambers, in which all the required reagents were preloaded in water-in-oil droplets. Read More

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September 2021

Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species.

Front Bioeng Biotechnol 2021 16;9:744198. Epub 2021 Sep 16.

GeneFluidics, Irwindale, CA, United States.

Novel molecular platforms are available for identifying (ID) the causative agents of microbial infections and generating antimicrobial susceptibility testing (AST) profiles, which can inform the suitable course of treatment. Many methods claim to perform AST in minutes or hours, often ignoring the need for time-consuming steps such as enrichment cultures and isolation of pure cultures. In clinical microbiology laboratories, an infectious microbial must first be cultured (overnight to days) and identified at the species level, followed by a subsequent AST with an additional turnaround time of 12-48 h due to the need for regrowth of the organism in the absence and presence of relevant antibiotics. Read More

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September 2021

A magnetic modulation biosensing-based molecular assay for rapid and highly sensitive clinical diagnosis of COVID-19.

J Mol Diagn 2021 Sep 29. Epub 2021 Sep 29.

Faculty of Engineering, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Max and Anna Webb Street, Ramat Gan, 5290002, Israel. Electronic address:

Rapid and sensitive detection of human pathogens, such as the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), is an urgent and challenging task for clinical laboratories. Currently, the gold standard test for SARS-CoV-2-specific ribonucleic acid (RNA) is based on reverse transcription quantitative polymerase chain reaction (RT-qPCR), which relies on target amplification by Taq polymerase and uses a fluorescent resonance energy transfer (FRET)-based hydrolysis probe. Although this method is accurate and specific, it is also time consuming. Read More

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September 2021

3D-printed BC collimation for neutron pressure cells.

Rev Sci Instrum 2021 Sep;92(9):093903

Energy and Transportation Science Division, Energy and Environmental Sciences Directorate, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.

A design for an incident-beam collimator for the Paris-Edinburgh pressure cell is described here. This design can be fabricated from reaction-bonded BC but also through fast turnaround, inexpensive 3D-printing. 3D-printing thereby also offers the opportunity of composite collimators whereby the tip closest to the sample can exhibit even better neutronic characteristics. Read More

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September 2021

Evaluation of the diagnostic accuracy of COVID-19 antigen tests: A systematic review and meta-analysis.

J Chin Med Assoc 2021 Sep 28. Epub 2021 Sep 28.

Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, ROC Department of Medical Research, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan, ROC Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, ROC Department of Urology, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan, ROC TMU Research Center of Urology and Kidney (TMU-RCUK), Taipei Medical University, Taipei, Taiwan, ROC Department of Public Health, College of Medicine, Taipei Medical University, Taipei, Taiwan, ROC Department of Laboratory Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan, ROC Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan, ROC. Department of Medicine, Cheng Hsin General Hospital, Taipei, Taiwan, ROC Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, ROC.

Background: The coronavirus disease 2019 (COVID-19) pandemic continues to affect countries worldwide. To inhibit the transmission of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), testing of patients, contact tracing, and quarantine of their close contacts have been used as major non-pharmaceutical interventions. The advantages of antigen tests, such as low cost and rapid turnaround, may allow for the rapid identification of larger numbers of infectious persons. Read More

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September 2021

Covid-19 vaccine management (Comirnaty and mrna-1273 Moderna) in a teaching hospital in Italy: a short report on the vaccination campaign.

Environ Health Prev Med 2021 Sep 30;26(1):99. Epub 2021 Sep 30.

Department of Translational Research and the New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.

Objectives: In this article, we aim to share our experience in the hospital reorganization made to conduct the SARS-CoV-2 vaccination campaign, based on the principles of flexibility and adaptability.

Study Design: A descriptive study.

Methods: The data concerning the organization of the vaccination campaign were taken from the operative protocol developed by the hospital dedicated task force, composed by experts in hygiene, public health, occupational medicine, pharmacists, nurses, hospital quality, and disaster managers. Read More

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September 2021

Metastatic NSCLCs With Limited Tissues: How to Effectively Identify Driver Alterations to Guide Targeted Therapy in Chinese Patients.

JTO Clin Res Rep 2021 May 24;2(5):100167. Epub 2021 Mar 24.

Department of Pathology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

Introduction: Molecular diagnostics of newly diagnosed patients with metastatic NSCLC (mNSCLC) with limited tissue samples often face several obstacles in routine practice using next-generation sequencing (NGS), mainly owing to insufficient tissue or DNA; thus, how to effectively identify the molecular profiling of these cases to accurately guide targeted therapy remains elusive. We evaluated whether an optimized workflow with the combined use of multiple technologies could be helpful.

Methods: Tissue NGS was used as the frontline method. Read More

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Computer-Aided Medical Microbiology Monitoring Tool: A Strategy to Adapt to the SARS-CoV-2 Epidemic and That Highlights RT-PCR Consistency.

Front Cell Infect Microbiol 2021 13;11:594577. Epub 2021 Sep 13.

Insitute of Microbiology, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland.

Since the beginning of the COVID-19 pandemic, important health and regulatory decisions relied on SARS-CoV-2 reverse transcription polymerase chain reaction (RT-PCR) results. Our diagnostic laboratory faced a rapid increase in the number of SARS-CoV-2 RT-PCR. To maintain a rapid turnaround time, we moved from a case-by-case validation of RT-PCR results to an automated validation and immediate results transmission to clinicians. Read More

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October 2021

ONTbarcoder and MinION barcodes aid biodiversity discovery and identification by everyone, for everyone.

BMC Biol 2021 09 29;19(1):217. Epub 2021 Sep 29.

Department of Biological Sciences, National University of Singapore, Singapore, Singapore.

Background: DNA barcodes are a useful tool for discovering, understanding, and monitoring biodiversity which are critical tasks at a time of rapid biodiversity loss. However, widespread adoption of barcodes requires cost-effective and simple barcoding methods. We here present a workflow that satisfies these conditions. Read More

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September 2021

Optimized Post-Vaccination Strategies and Preventative Measures for SARS-CoV-2.

medRxiv 2021 Sep 21. Epub 2021 Sep 21.

Introduction: Since March of 2020, over 210 million SARS-CoV-2 cases have been reported and roughly five billion doses of a SARS-CoV-2 vaccine have been delivered. The rise of the more infectious delta variant has recently indicated the value of reinstating previously relaxed non-pharmacological and test-driven preventative measures. These efforts have been met with resistance, due, in part, to a lack of site-specific quantitative evidence which can justify their value. Read More

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September 2021

Integrated Microfluidic-Based Platforms for On-Site Detection and Quantification of Infectious Pathogens: Towards On-Site Medical Translation of SARS-CoV-2 Diagnostic Platforms.

Micromachines (Basel) 2021 Sep 7;12(9). Epub 2021 Sep 7.

School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4L8, Canada.

The rapid detection and quantification of infectious pathogens is an essential component to the control of potentially lethal outbreaks among human populations worldwide. Several of these highly infectious pathogens, such as Middle East respiratory syndrome (MERS) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have been cemented in human history as causing epidemics or pandemics due to their lethality and contagiousness. SARS-CoV-2 is an example of these highly infectious pathogens that have recently become one of the leading causes of globally reported deaths, creating one of the worst economic downturns and health crises in the last century. Read More

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September 2021

Design and Validation of a Custom NGS Panel Targeting a Set of Lysosomal Storage Diseases Candidate for NBS Applications.

Int J Mol Sci 2021 Sep 17;22(18). Epub 2021 Sep 17.

Institute for Biomedical Research and Innovation, National Research Council, 95126 Catania, Italy.

Lysosomal storage diseases (LSDs) are a heterogeneous group of approximately 70 monogenic metabolic disorders whose diagnosis represents an arduous challenge for clinicians due to their variability in phenotype penetrance, clinical manifestations, and high allelic heterogeneity. In recent years, the approval of disease-specific therapies and the rapid emergence of novel rapid diagnostic methods has opened, for a set of selected LSDs, the possibility for inclusion in extensive national newborn screening (NBS) programs. Herein, we evaluated the clinical utility and diagnostic validity of a targeted next-generation sequencing (tNGS) panel (called NBS_LSDs), designed ad hoc to scan the coding regions of six genes (, , , , , ) relevant for a group of LSDs candidate for inclusion in national NBS programs (MPSI, Pompe, Fabry, Krabbe, Niemann Pick A-B and Gaucher diseases). Read More

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September 2021