13,770 results match your criteria tunel staining


Thrombomodulin-mediated Inhibition of Neutrophil Extracellular Trap Formation Alleviates Hepatic Ischemia/Reperfusion Injury by Blocking TLR4 in Rats Subjected to Liver Transplantation.

Transplantation 2021 Sep 16. Epub 2021 Sep 16.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China. West China School of Medicine, Sichuan University, Chongqing, People's Republic of China.

Background: Hepatic ischemia/reperfusion injury (IRI) is an unavoidable outcome of liver transplantation, during which neutrophil extracellular traps (NETs) may play a critical role in the IRI-induced immune response to inflammation. The purpose of this study was to identify the function of recombinant human thrombomodulin (rTM) in the remission of hepatic IRI after liver transplantation and elucidate the specific mechanism.

Methods: NET formation was detected in the serum of liver transplantation patients and rats following liver transplantation. Read More

View Article and Full-Text PDF
September 2021

Exosomal miR-132-3p from mesenchymal stem cells alleviated LPS-induced acute lung injury by repressing TRAF6.

Autoimmunity 2021 Sep 17:1-11. Epub 2021 Sep 17.

Department of Respiratory Medicine, The First Affiliated Hospital of Hebei North University, Zhangjiakou, P. R. China.

Exosomes isolated from mesenchymal stem cells (MSC) had shown beneficial effect on acute lung injury (ALI). However, the effective components in MSC-derived exosomes need further investigation. ALI mice model was established by lipopolysaccharide (LPS) injection. Read More

View Article and Full-Text PDF
September 2021

[Necrostatin-1 promotes locomotor recovery after spinal cord injury through inhibiting apoptosis and M1 polarization of microglia/macrophage in mice].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Sep;37(9):775-780

Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710054, China. *Corresponding author, E-mail:

Objective To investigate the effect of necrostatin-1 on locomotor recovery after spinal cord injury (SCI) in mice, and to explore the role of apoptosis and M1 type-microglia/macrophage-mediated pro-inflammation in the protective effect. Methods Male C57BL/6 mice were randomly divided into four groups: control group, necrostatin-1 group, SCI model group, necrostatin-1-treated group after SCI, with 20 mice in each. For SCI model group, mice were anesthetized with 10 g/L pentobarbital sodium with a dose of 8 mL/kg. Read More

View Article and Full-Text PDF
September 2021

Dexmedetomidine alleviates traumatic spinal cord injury in rats via inhibiting apoptosis induced by endoplasmic reticulum stress.

Neurol Res 2021 Sep 17:1-10. Epub 2021 Sep 17.

Department of Anesthesiology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China.

Objective: To investigate the protective effect of dexmedetomidine (Dex) on traumatic spinal cord injury (TSCI) and to evaluate the involvement of inhibition of endoplasmic reticulum (ER) stress response in the potential mechanism.

Method: Sprague-Dawley rats were randomly divided into five groups. The hind limb locomotor function of rats was evaluated at 1, 3 and 7 days after the operation. Read More

View Article and Full-Text PDF
September 2021

Characterization of the Developing Lacunocanalicular Network During Fracture Repair.

JBMR Plus 2021 Sep 12;5(9):e10525. Epub 2021 Jul 12.

Institute for Biomechanics ETH Zurich Zurich Switzerland.

Fracture repair is a normal physiological response to bone injury. During the process of bony callus formation, a lacunocanalicular network (LCN) is formed de novo that evolves with callus remodeling. Our aim was the longitudinal assessment of the development and evolution of the LCN during fracture repair. Read More

View Article and Full-Text PDF
September 2021

Dandelion sterol improves diabetes mellitus-induced renal injury in in vitro and in vivo study.

Food Sci Nutr 2021 Sep 28;9(9):5183-5197. Epub 2021 Jul 28.

Department of Pathology The First Affiliated Hospital of Harbin Medical University Harbin China.

The purpose of our research was to evaluate Dandelion sterol's treatment effects on diabetes mellitus-induced renal injury in in vitro and in vivo study. The rats were divided into five groups as normal control (Ctrl), diabetic nephropathy model (Model), Dandelion sterol low-dose treated (Dan-Low), Dandelion sterol middle-dose treated (Dan-Middle), and Dandelion sterol high-dose treated (Dan-High). Measuring serum TNF-α, IL-1β, and IL-6 concentrations by Elisa assay, evaluate kidney pathology by HE staining, kidney cell apoptosis of TUNEL, TLR4, and NF-κB(p65) proteins expression by IHC assay, and relative gene expressions by RT-qPCR assay. Read More

View Article and Full-Text PDF
September 2021

Quercetin alleviates chronic renal failure by targeting the PI3k/Akt pathway.

Bioengineered 2021 Dec;12(1):6538-6558

Division of Nephrology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong, Guangzhou, China.

Chronic renal failure (CRF) threatens human health greatly and attracts worldwide concerns of health professionals in the public health sector. In our preliminary study, we found that Compound capsule (Shengqing Jiangzhuo Capsule, SQJZJN) had a significant therapeutic effect on CRF. Quercetin is one of the main components of this Compound capsule. Read More

View Article and Full-Text PDF
December 2021

Effects of Long-Term Exposure to L-Band High-Power Microwave on the Brain Function of Male Mice.

Biomed Res Int 2021 4;2021:2237370. Epub 2021 Sep 4.

Department of Radiation Medicine and Protection, Faculty of Preventive Medicine, Airforce Medical University, Xi'an, Shaanxi 710032, China.

Currently, the impact of electromagnetic field (EMF) exposure on the nervous system is an increasingly arousing public concern. The present study was designed to explore the effects of continuous long-term exposure to L-band high-power microwave (L-HPM) on brain function and related mechanisms. Forty-eight male Institute of Cancer Research (ICR) mice were exposed to L-HPM at various power densities (0. Read More

View Article and Full-Text PDF
September 2021

Effects of acrylonitrile on apoptosis of rat cerebral nerve cells.

Int J Clin Exp Pathol 2021 15;14(8):908-914. Epub 2021 Aug 15.

Department of Occupational Health, Jinshan Hospital, Fudan University Shanghai, China.

The brain, especially the hippocampus, is sensitive to damage caused by anoxic chemicals. In this study, we established a rat model of acrylonitrile poisoning with administration by gavage, aiming to determine the influence of acrylonitrile on rat cerebral nerve cells. Transmission electron microscopy observation and TdT-mediated dUTP nick-end labelling (TUNEL) staining were used to explore preliminarily the apoptotic changes of cerebral nerve cells. Read More

View Article and Full-Text PDF

The Jieduan-Niwan (JDNW) Formula Ameliorates Hepatocyte Apoptosis: A Study of the Inhibition of E2F1-Mediated Apoptosis Signaling Pathways in Acute-on-Chronic Liver Failure (ACLF) Using Rats.

Drug Des Devel Ther 2021 8;15:3845-3862. Epub 2021 Sep 8.

Department of Hepatology, School of Traditional Chinese Medicine, Capital Medical University, Beijing, People's Republic of China.

Background: Acute-on-chronic liver failure (ACLF) is a severe, complicated human disease. E2F1-mediated apoptosis plays an important role in ACLF development. Jieduan-Niwan (JDNW) formula, a traditional Chinese medicine (TCM), has shown remarkable clinical efficacy in ACLF treatment. Read More

View Article and Full-Text PDF
September 2021

Preferable Transplant Site for Hepatocyte Transplantation in a Rat Model.

Cell Transplant 2021 Jan-Dec;30:9636897211040012

Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.

Intraportal injection is regarded as the current standard procedure of hepatocyte transplantation (HTx). In islet transplantation, which shares many aspects with HTx, recent studies have clarified that instant blood-mediated inflammatory reaction (IBMIR), characterized by strong innate immune responses, can cause poor engraftment, so other transplant sites to avoid such a reaction have been established. Although IBMIR was reported to occur in HTx, few reports have evaluated alternative transplant sites for HTx. Read More

View Article and Full-Text PDF
September 2021

The mitochondria-targeting antioxidant MitoQ alleviated Lipopolysaccharide/ D-galactosamine-induced acute liver injury in mice.

Immunol Lett 2021 Sep 12. Epub 2021 Sep 12.

Laboratory of Stem cell and Tissue Engineering, Chongqing Medical University, Chongqing, China; Department of Pathophysiology, Chongqing Medical University, Chongqing, China. Electronic address:

The mitochondria are the primary source of reactive oxygen species (ROS) under pathological condition, but the significance of mitochondrial ROS in the development of Lipopolysaccharide (LPS)/D-galactosamine (D-Gal)-induced acute liver injury remains unclear. In the present study, the level of mitochondrial ROS in LPS/D-Gal has been determined by MitoSox staining and the potential roles of mitochondrial ROS in LPS/D-Gal-induced liver injury have been investigated by using the mitochondria-targeting antioxidant MitoQ. The results indicated that LPS/D-Gal exposure induced the generation of mitochondrial ROS while treatment with MitoQ reduced the level of mitochondrial ROS. Read More

View Article and Full-Text PDF
September 2021

Repair of spinal cord injury in rats via exosomes from bone mesenchymal stem cells requires sonic hedgehog.

Regen Ther 2021 Dec 1;18:309-315. Epub 2021 Sep 1.

Department of Spine Surgery, Guizhou Province Osteological Hospital, Guiyang, 550002, China.

Objective: The loss of neural ability leading to subsequent diminishing of motor function and the impairment below the location of the injury is a result of the SCI (Spinal Cord Injury). Among the many therapeutic agents for SCI, the exosomes considered as extracellular vesicles seem to be the most promising. Sonic Hedgehog (Shh) is an exosome-carrying protein. Read More

View Article and Full-Text PDF
December 2021

The role of XBP-1-mediated unfolded protein response in colorectal cancer progression-a regulatory mechanism associated with lncRNA-miRNA-mRNA network.

Cancer Cell Int 2021 Sep 14;21(1):488. Epub 2021 Sep 14.

Department of General Surgery, Huangyan Hospital of Wenzhou Medical University, Taizhou First People's Hospital, No. 218, Hengjie Road, Huangyan District, Taizhou City, 318020, Zhejiang Province, China.

Background: We aim to identify the expression and analyze the molecular action of dysregulated lncRNA-miRNA mediated by XBP-1 in colorectal cancer (CRC).

Methods: Here, we identified XBP-1-mediated dysregulated lncRNAs and miRNAs in CRC by bioinformatics analysis. The expression level of lncRNAs and miRNA was measured using quantitative real time PCR, and the expression of XBP-1, as well as apoptosis-related proteins, were detected by western blot. Read More

View Article and Full-Text PDF
September 2021

Saikosaponin-A induces apoptosis of cervical cancer through mitochondria- and endoplasmic reticulum stress-dependent pathway and : involvement of PI3K/AKT signaling pathway.

Cell Cycle 2021 Sep 14:1-12. Epub 2021 Sep 14.

Department of Pharmacology, Institute of Traditional Chinese Medicine and New Pharmacy Development, Guangdong Medical University, Dongguan, China.

Cervical cancer causes considerable mortality in women worldwide. Saikosaponin-A, a triterpenoid glycoside isolated from , has been proven to exert anti-cancer property. In this study, we evaluated the possibility of saikosaponin-A on cervical cancer and . Read More

View Article and Full-Text PDF
September 2021

LncRNA HOXA-AS2 Promotes Oral Squamous Cell Proliferation, Migration, and Invasion via Upregulating EZH2 as an Oncogene.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211039109

117878The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, P.R. China.

Oral squamous cell carcinoma (OSCC) is one of the most common types of cancer worldwide. Accumulating evidence has shown that long noncoding RNAs (lncRNAs) serve important roles in the development of OSCC. The purpose of this study was to investigate the biological function and underlying regulatory mechanism of lncRNA homeobox A cluster antisense RNA2 (HOXA-AS2) in OSCC. Read More

View Article and Full-Text PDF
September 2021

Nesfatin-1 protects H9c2 cardiomyocytes against cobalt chloride-induced hypoxic injury by modulating the MAPK and Notch1 signaling pathways.

J Biol Res (Thessalon) 2021 Sep 13;28(1):21. Epub 2021 Sep 13.

Department of Cardiology, The People's Hospital of Jiulongpo, No. 31 Baixin Road, Baishiyi, Jiulongpo, 401329, Chongqing, People's Republic of China.

Background: This study aimed to explore the effect of nesfatin-1 on cobalt chloride (CoCl)-induced hypoxic injury in cardiomyocyte H9c2 cells.

Methods: H9c2 cardiomyocytes were induced by different concentrations of CoCl to mimic the hypoxia condition. Cell viability was detected by MTT assay. Read More

View Article and Full-Text PDF
September 2021

The roles of polymorphonuclear myeloid-derived suppressor cells in endometriosis.

J Reprod Immunol 2021 Sep 4;148:103371. Epub 2021 Sep 4.

Department of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, 113-8655, Japan.

Objectives: This study aimed to determine the systemic and local proportions, focal localization, and characteristics of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) in endometriosis.

Study Design: Peripheral blood and peritoneal fluid were obtained from patients with a benign gynecologic condition (controls) or endometriosis. PMN-MDSCs were defined as CD33HLA-DRCD14CD15 and monocytic (M)-MDSCs were defined as CD33HLA-DRCD14CD15, and were identified using flowcytometry. Read More

View Article and Full-Text PDF
September 2021

Berberine exerts neuroprotective activities against cerebral ischemia/reperfusion injury through up-regulating PPAR-γ to suppress NF-κB-mediated pyroptosis.

Brain Res Bull 2021 Sep 10. Epub 2021 Sep 10.

Department of Neurology (Six), the Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China. Electronic address:

Objective: Berberine (BBR) is an anti-inflammatory alkaloid compound extracted from herbs. The purpose of this study is to probe the possible effect and the mechanism of BBR against cerebral ischemia/reperfusion (I/R) injury.

Methods: In vitro oxygen and glucose deprivation (OGD) model was established on neurons from rat hippocampus, which was then subjected to BBR, IVA337 (PPAR-γ agonist), or GW9662 (PPAR-γ antagonist) treatment, to identify their effects on neuronal pyroptosis. Read More

View Article and Full-Text PDF
September 2021

Astrocyte-derived exosomes protect hippocampal neurons after traumatic brain injury by suppressing mitochondrial oxidative stress and apoptosis.

Aging (Albany NY) 2021 Sep 13;13(undefined). Epub 2021 Sep 13.

Department of Endocrinology, Tangshan Gongren Hospital, Tangshan, Hebei 063000, China.

In this study, we investigated the mechanisms through which astrocyte-derived exosomes (AS-Exos) alleviate traumatic brain injury (TBI)-induced neuronal defects in TBI model rats and mice. Treatment with AS-Exos alleviated neurobehavioral deficits, cognitive impairment, and brain edema in TBI rats. AS-Exos also significantly reduced neuronal cell loss and atrophy in the TBI rats. Read More

View Article and Full-Text PDF
September 2021

The anti-obesity effects of rhein on improving insulin resistance (IR) and blood lipid levels are involved in endoplasmic reticulum stress (ERs), inflammation, and oxidative stress in vivo and vitro.

Authors:
Li Ji Huan Gu

Bioengineered 2021 Dec;12(1):5797-5813

Department of Cardiology of Integrated Traditional Chinese and Western Medicine China-Japan Friendship Hospital, BeiJing, China.

Rhein extensive biological effects including anti-inflammatory, antioxidant stress, and improving glucose and lipid metabolism. In the present study, the effects of rhein were examined on endoplasmic reticulum stress (ERs) and inflammation in obesity-induced rats. SD rats were fed with a normal diet or a high-fat diet. Read More

View Article and Full-Text PDF
December 2021

CTRP6(C1q/Tumor Necrosis Factor (TNF)-related protein-6) alleviated the sevoflurane induced injury of mice central nervous system by promoting the expression of p-Akt (phosphorylated Akt).

Authors:
Zhiwen Liu Bin Yang

Bioengineered 2021 Dec;12(1):5716-5726

Department of Anesthesiology, The Second Hospital, University to South China Hengyang Cty, Hengyang City, Hunan Province, China.

Postoperative cognitive impairment and nervous system damage caused by anesthetics seriously affect patient's postoperative recovery. Recent study has revealed that CTRP6 could alleviate apoptosis, inflammation and oxidative stress of nerve cells, thereby relieving nervous system damage induced by cerebral ischemia reperfusion. However, whether CTRP6 could relieve sevoflurane induced central nervous system injury is unclear. Read More

View Article and Full-Text PDF
December 2021

Endoplasmic reticulum stress is involved in retinal injury induced by repeated transient spikes of intraocular pressure.

J Zhejiang Univ Sci B 2021 Sept 15;22(9):746-756

State Key Laboratory of Ophthalmology, Department of Glaucoma, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.

Clinically, a large proportion of glaucoma patients undergo repeated intraocular pressure (IOP) spike (Spike IOP) attacks during their sleep, which may facilitate retinopathy. In this study, we established a mouse model of repeated transient Spike IOP to investigate the direct damage to the retina following Spike IOP attacks, and elucidated the underlying molecular mechanism. We analyzed the changes in the number of retinal ganglion cells (RGCs) via immunofluorescence. Read More

View Article and Full-Text PDF
September 2021

Clemastine Fumarate Attenuates Myocardial Ischemia Reperfusion Injury Through Inhibition of Mast Cell Degranulation.

Front Pharmacol 2021 27;12:704852. Epub 2021 Aug 27.

Shandong Provincial Medicine and Health Key Laboratory of Clinical Anesthesia, School of Anesthesiology, Weifang Medical University, Weifang, China.

Mast cell (MC) activation is associated with myocardial ischemia reperfusion injury (MIRI). Suppression of MC degranulation might be a target of anti-MIRI. This study aimed to determine whether clemastine fumarate (CLE) could attenuate MIRI by inhibiting MC degranulation. Read More

View Article and Full-Text PDF

Downregulation of miR-23b by transcription factor c-Myc alleviates ischemic brain injury by upregulating Nrf2.

Int J Biol Sci 2021 26;17(13):3659-3671. Epub 2021 Aug 26.

Jilin University, Changchun 130000, P. R. China.

Ischemic brain injury (IBI) is a common acute cerebral vessel disease that occurs secondary to blockage in arteries, mainly characterized by insufficient blood supply to the brain. The transcription factor c-Myc in IBI continues to be implicated in numerous studies. This study was conducted with emphasis placed on the underlying mechanism of c-Myc in IBI. Read More

View Article and Full-Text PDF

Long Non-Coding RNA Facilitates Cell Proliferation and Induces Apoptosis in Colorectal Cancer via miR-519b/ZNF277 Axis.

Onco Targets Ther 2021 1;14:4693-4703. Epub 2021 Sep 1.

Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, People's Republic of China.

Introduction: Long non-coding RNAs (LncRNAs) play a critical role in development and progression of various cancers. More and more researchers pay attention to the effect of lncRNA on regulating the cancer. However, the function and mechanism of in colorectal cancer have not been studied. Read More

View Article and Full-Text PDF
September 2021

Resveratrol-modified mesoporous silica nanoparticle for tumor-targeted therapy of gastric cancer.

Bioengineered 2021 Dec;12(1):6343-6353

Department of Oncology, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.

Resveratrol (Res) has been shown to exhibit anti-cancer properties in gastric cancer. However, its clinical application is limited by its poor pharmacokinetics, stability, and low solubility. Hence, this study aimed to explore and verify a better delivery system for gastric cancer therapy. Read More

View Article and Full-Text PDF
December 2021

Effects of high temperature and high humidity stress on the negative feedback regulation of hippocampus on HPA axis in rats.

Neuro Endocrinol Lett 2021 Sep 10;42(5):312-320. Epub 2021 Sep 10.

Rheumatology Center of Integrated Medicine, The General Hospital of Western Theater Command, PLA, Chengdu, 610083, China.

Objective: To investigate the negative feedback regulation from rat hippocampus on hypothalamic-pituitary-adrenal (HPA) axis under high temperature and high humidity stress.

Methods: Thirty (30) SD male rats were randomly divided into three groups: control group, high temperature and high humidity group, drug intervention group. The rats in control group were kept in the environment with temperature of 24 ± 1°C and humidity of 50 ± 5%, without any stimulation. Read More

View Article and Full-Text PDF
September 2021

MKRN1 Ubiquitylates p21 to Protect against Intermittent Hypoxia-Induced Myocardial Apoptosis.

Oxid Med Cell Longev 2021 30;2021:9360339. Epub 2021 Aug 30.

Department of Pulmonary and Critical Care Medicine, The First Hospital of Lanzhou University, Lanzhou 730000, China.

Although chronic intermittent hypoxia- (IH-) induced myocardial apoptosis is an established pathophysiological process resulting in a poor prognosis for patients with obstructive sleep apnea syndrome, its underlying mechanism remains unclear. This study is aimed at exploring the role of makorin ring finger protein 1 (MKRN1) in IH-induced myocardial apoptosis and elucidating its molecular activity. First, the GSE2271 dataset was downloaded from the Gene Expression Omnibus database to identify the differentially expressed genes. Read More

View Article and Full-Text PDF

Angiotensin-converting enzyme 2 alleviates pulmonary artery hypertension through inhibition of focal adhesion kinase expression.

Exp Ther Med 2021 Oct 12;22(4):1165. Epub 2021 Aug 12.

Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221006, P.R. China.

Focal adhesion kinase (FAK) is an important therapeutic target in pulmonary artery hypertension (PAH); however, the mechanism of its activation remains unknown. The present study aimed to investigate whether angiotensin-converting enzyme 2 (ACE2) could regulate FAK and alleviate PAH in a rat model of PAH established with a single administration of monocrotaline followed by continuous hypoxia treatment. In the current study, right ventricular pressure, body weight and the right ventricular hypertrophy index were measured, and hematoxylin-eosin staining was performed on lung tissues to determine whether the modeling was successful. Read More

View Article and Full-Text PDF
October 2021