30 results match your criteria tryp genes

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Sirolimus enhances the protection achieved by a DNA vaccine against Leishmania infantum.

Parasit Vectors 2020 Jun 9;13(1):294. Epub 2020 Jun 9.

Departament de Farmacologia, de Terapèutica i de Toxicologia, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.

Background: Leishmaniases are a group of neglected tropical parasitic diseases, mainly affecting vulnerable populations of countries with poor socioeconomic status. Development of efficient vaccines is a priority due to the increasing incidence of drug resistance and toxicity to current treatments. In the search for a safe and efficient protective vaccine for human and dog visceral leishmaniases, we analyzed the suitability of the immunomodulatory drug sirolimus (SIR) to boost a preventive DNA vaccine against leishmaniasis. Read More

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Deletion of S-Layer Associated Ig-Like Domain Protein Disrupts the Cell Surface.

Front Microbiol 2020 17;11:345. Epub 2020 Mar 17.

Genomic Sciences Graduate Program, North Carolina State University, Raleigh, NC, United States.

Bacterial surface-layers (S-layers) are crystalline arrays of repeating proteinaceous subunits that coat the exterior of many cell envelopes. S-layers have demonstrated diverse functions in growth and survival, maintenance of cell integrity, and mediation of host interactions. Additionally, S-layers can act as scaffolds for the outward display of auxiliary proteins and glycoproteins. Read More

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Comparison of gene expression of pyruvate kinase and tryparedoxin peroxidase in metacyclic promastigote forms of Leishmania (L.) tropica and L. major by real-time PCR

Ann Parasitol 2020 ;66(1):13-18

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Two predominant forms of cutaneous leishmaniosis are anthroponotic CL (ACL) and zoonotic CL (ZCL) caused by Leishmania (L.) tropica and L. major in Iran and many countries, respectively. Read More

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Global gene expression changes induced by knockout of a protease gene cluster in Helicoverpa armigera with CRISPR/Cas9.

J Insect Physiol 2020 04 20;122:104023. Epub 2020 Feb 20.

College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

Helicoverpa armigera is one of the most serious agricultural insect pests of global importance. It is highly polyphagous and depends on digestive serine proteases to degrade proteins to peptides and to amino acids. H. Read More

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Comparison of the anti-colitis activities of Qing Dai/Indigo Naturalis constituents in mice.

J Pharmacol Sci 2020 Apr 22;142(4):148-156. Epub 2020 Jan 22.

Pharmacology Laboratory, Department of Biomedical Sciences, College of Life Sciences, Ritsumeikan University, Shiga, 525-8577, Japan. Electronic address:

Qing Dai/Indigo Naturalis (QD) has been shown to ameliorate ulcerative colitis (UC) in clinical trials; however, its mechanism remains elusive. This study investigates the effects of QD on murine dextran sulfate sodium salt-induced colitis. Oral administration of QD protected the animals from colitis as manifested by weight loss, diarrhea, and rectal bleeding. Read More

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Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.

Sci Rep 2019 04 23;9(1):6464. Epub 2019 Apr 23.

Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs; Key Laboratory of Fishery Ecology and Environment, Guangdong Province, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, P.R. China.

Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals. However, the roles of RS on shrimp intestine health is unknown. In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei. Read More

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Endocrine-disrupting chemicals impair the innate immune prophenoloxidase system in the intertidal mud crab, Macrophthalmus japonicus.

Fish Shellfish Immunol 2019 Apr 22;87:322-332. Epub 2019 Jan 22.

Faculty of Marine Technology, Chonnam National University, Yeosu, 550-749, South Korea. Electronic address:

Endocrine-disrupting chemicals (EDCs), xenobiotics that interfere with endogenous hormone function, have been studied for their impacts in aquatic environments. However, there is limited information about the potentially hazardous impact of bisphenol A (BPA) and di-(2-ethylhexyl) phthalate (DEHP) on the marine environment. The aim of this study was to investigate the effects of BPA and DEHP on the immune response of the intertidal mud crab, Macrophthalmus japonicus. Read More

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Changes of exoskeleton surface roughness and expression of crucial participation genes for chitin formation and digestion in the mud crab (Macrophthalmus japonicus) following the antifouling biocide irgarol.

Ecotoxicol Environ Saf 2016 Oct 17;132:186-95. Epub 2016 Jun 17.

Faculty of Marine Technology, Chonnam National University, Chonnam 550-749, South Korea. Electronic address:

Irgarol is a common antifoulant present in coastal sediment. The mud crab Macrophthalmus japonicus is one of the most abundant of the macrobenthos in the costal environment, and its exoskeleton has a protective function against various environmental threats. We evaluated the effects of irgarol toxicity on the exoskeleton of M. Read More

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October 2016

Comparative ontogenetic development of two marine teleosts, gilthead seabream and European sea bass: New insights into nutrition and immunity.

Dev Comp Immunol 2016 12 15;65:1-7. Epub 2016 Jun 15.

Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain. Electronic address:

Gilthead seabream and European sea bass are two of the most commonly farmed fish species. Larval development is critical to ensure high survival rates and thus avoid unacceptable economic losses, while nutrition and immunity are also important factors. For this reason this paper evaluates the ontogenetic development of seabream and sea bass digestive and immune systems from eggs to 73 days post-fertilisation (dpf) by assessing the expression levels of some nutrition-relevant (tryp, amya, alp and pept1) and immune-relevant (il1b, il6, il8, tnfa, cox2, casp1, tf, nccrp1, ighm and ight) genes. Read More

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December 2016


Arch Insect Biochem Physiol 2016 Apr 26;91(4):189-209. Epub 2015 Nov 26.

Department of Agronomy & Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

Sunn pest, Eurygaster integriceps, is a serious pest of cereals in the wide area of the globe from Near and Middle East to East and South Europe and North Africa. This study described for the first time, identification of E. integriceps trypsin serine protease and cathepsin-L cysteine, transcripts involved in digestion, which might serve as targets for pest control management. Read More

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Identification of potential markers and sensitive tissues for low or high salinity stress in an intertidal mud crab (Macrophthalmus japonicus).

Fish Shellfish Immunol 2014 Dec 18;41(2):407-16. Epub 2014 Sep 18.

Faculty of Marine Technology, Chonnam National University, Chonnam 550-749, Republic of Korea. Electronic address:

Macrophthalmus japonicus is an intertidal mud crab is an ecologically important species in Korea, can tolerate a wide range of natural and anthropogenic stressors. Environmental changes especially salinity cause physiological stress to the marine habitats. Differential gene transcription of M. Read More

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December 2014

Characterization and phylogenetic analysis of allergenic Tryp_alpha_amyl protein family in plants.

J Agric Food Chem 2014 Jan 20;62(1):270-8. Epub 2013 Dec 20.

National Center for Molecular Characterization of Genetically Modified Organisms, State Key Laboratory of Hybrid Rice, School of Life Science and Biotechnology, Shanghai Jiao Tong University , 800 Dongchuan Road, Shanghai 200240, People's Republic of China.

Most known allergenic proteins in rice ( Oryza sativa ) seed belong to the Tryp_alpha_amyl family (PF00234), but the sequence characterization and the evolution of the allergenic Tryp_alpha_amyl family members in plants have not been fully investigated. In this study, two specific motifs were found besides the common alpha-amylase inhibitors (AAI) domain from the allergenic Tryp_alpha_amyl family members in rice seeds (trRSAs). To understand the evolution and functional importance of the Tryp_alpha_amy1 family and the specific motifs for the allergenic one, a BLAST search identified 75 homologous proteins of trRSAs (trHAs) from 22 plant species including main crops such as rice, maize ( Zea mays ), wheat ( Triticum aestivum ), and sorghum ( Sorghum bicolor ) from all available sequences in the public databases. Read More

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January 2014

Evaluation of potential reference genes for use in gene expression studies in the conifer pathogen (Heterobasidion annosum).

Mol Biol Rep 2013 Jul 5;40(7):4605-11. Epub 2013 May 5.

Department of Forest Sciences, University of Helsinki, Helsinki, Finland.

The basidiomycete Heterobasidion annosum is the causative agent of butt and root rot disease of conifer trees and it's one of the most destructive conifer pathogen in the northern hemisphere. Because of the intrinsic difficulties in genome manipulation in this fungus, most studies have been focused on gene expression analysis using quantitative real time polymerase chain reaction (qPCR). qPCR is a powerful technique but its reliability resides in the correct selection of a set of reference genes used in the data normalization. Read More

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Head-to-head comparison of three vaccination strategies based on DNA and raw insect-derived recombinant proteins against Leishmania.

PLoS One 2012 7;7(12):e51181. Epub 2012 Dec 7.

LeishLAB-Servei d'Anàlisi de Fàrmacs, Departament de Farmacologia, de Terapèutica i de Toxicologia, Edifici V, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.

Parasitic diseases plague billions of people among the poorest, killing millions annually, and causing additional millions of disability-adjusted life years lost. Leishmaniases affect more than 12 million people, with over 350 million people at risk. There is an urgent need for efficacious and cheap vaccines and treatments against visceral leishmaniasis (VL), its most severe form. Read More

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Trypanosomatid comparative genomics: Contributions to the study of parasite biology and different parasitic diseases.

Genet Mol Biol 2012 Jan 20;35(1):1-17. Epub 2012 Jan 20.

Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.

In 2005, draft sequences of the genomes of Trypanosoma brucei, Trypanosoma cruzi and Leishmania major, also known as the Tri-Tryp genomes, were published. These protozoan parasites are the causative agents of three distinct insect-borne diseases, namely sleeping sickness, Chagas disease and leishmaniasis, all with a worldwide distribution. Despite the large estimated evolutionary distance among them, a conserved core of ~6,200 trypanosomatid genes was found among the Tri-Tryp genomes. Read More

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January 2012

Bacterial mutagenicity assays: test methods.

David Gatehouse

Methods Mol Biol 2012 ;817:21-34

Old Barn, Cherry Orchard Lane Wyddial, Hertfordshire, SG9 0EN, UK.

The most widely used assays for detecting chemically induced gene mutations are those employing bacteria. The plate incorporation assay using various Salmonella typhimurium LT2 and E. coli WP2 strains is a short-term bacterial reverse mutation assay specifically designed to detect a wide range of chemical substances capable of causing DNA damage leading to gene mutations. Read More

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An essential nuclear protein in trypanosomes is a component of mRNA transcription/export pathway.

PLoS One 2011 8;6(6):e20730. Epub 2011 Jun 8.

Departamento de Biologia Celular e Molecular, Universidade Federal do Paraná, Curitiba, Brazil.

In eukaryotic cells, different RNA species are exported from the nucleus via specialized pathways. The mRNA export machinery is highly integrated with mRNA processing, and includes a different set of nuclear transport adaptors as well as other mRNA binding proteins, RNA helicases, and NPC-associated proteins. The protozoan parasite Trypanosoma cruzi is the causative agent of Chagas disease, a widespread and neglected human disease which is endemic to Latin America. Read More

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October 2011

A trypsin homolog in amphioxus: expression, enzymatic activity and evolution.

Mol Biol Rep 2012 Feb 29;39(2):1745-53. Epub 2011 May 29.

Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Room 205, Ke Xue Guan, 5 Yushan Road, Qingdao 266003, China.

Trypsin has been documented in a variety of species including both vertebrates and invertebrates, but little is known about it in amphioxus, a model organism for insights into the origin and evolution of vertebrates. Here we identified a trypsin gene in Branchiostoma japonicum. The cDNA was 978 bp long with an ORF encoding a deduced protein of 272 amino acids. Read More

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February 2012

A profound role for the expansion of trypsin-like serine protease family in the evolution of hematophagy in mosquito.

Mol Biol Evol 2009 Oct 3;26(10):2333-41. Epub 2009 Jul 3.

State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.

The trypsin-like serine protease (Tryp_SPc) family is ubiquitous in animals and plays diverse roles, especially in the digestive system, in different phyla. In the mosquito, some Tryp_SPc proteases make important contributions to the digestion of the blood meal. Here, we have defined the complete Tryp_SPc gene repertoire in the genome of the malaria mosquito, a repertoire that has expanded remarkably compared with that of Drosophila. Read More

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October 2009

[Effect of Cry1Ca7 protein modified by site-directed mutagenesis on inhibiting Spodoptera exigua Hübner].

Wei Sheng Wu Xue Bao 2008 Jun;48(6):733-8

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China.

Objective: To obtain the mutants with different toxicity from the wild-type CrylCa7.

Methods: Insecticidal crystal protein CrylCa7 from Bacillus thuringiensis which is highly toxic to Spodoptera exigua, an important agricultural pest in China, and we mutated this toxin by over-lapping extensive PCR method in different domains to obtain 11 chimeric mutants.

Results: The results of bioassays against Spodoptera exigua neonates showed that several conserved amino acid sites were crucial to insects. Read More

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Evolutionary consequences of a large duplication event in Trypanosoma brucei: chromosomes 4 and 8 are partial duplicons.

Andrew P Jackson

BMC Genomics 2007 Nov 23;8:432. Epub 2007 Nov 23.

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK.

Background: Gene order along the genome sequence of the human parasite Trypanosoma brucei provides evidence for a 0.5 Mb duplication, comprising the 3' regions of chromosomes 4 and 8. Here, the principal aim was to examine the contribution made by this duplication event to the T. Read More

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November 2007

Characterization of a male sterile related gene BcMF15 from Brassica campestris ssp. chinensis.

Mol Biol Rep 2009 Feb 22;36(2):307-14. Epub 2007 Nov 22.

Lab of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310029, P.R. China.

Data from cDNA-AFLP analysis based on the genome-wide transcriptional profiling on the flower buds of the male meiotic cytokinesis (mmc) mutant and its wild-type of Brassica campestris L. ssp. chinensis Makino, syn. Read More

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February 2009

A comparative study of type I and type II tryparedoxin peroxidases in Leishmania major.

FEBS J 2007 Nov 8;274(21):5643-58. Epub 2007 Oct 8.

Wellcome Trust Biocentre, University of Dundee, Dundee, UK.

The genome of Leishmania major, the causative agent of cutaneous leishmaniasis, contains three almost identical genes encoding putative glutathione peroxidases, which differ only at their N- and C-termini. Because the gene homologues are essential in trypanosomes, they may also represent potential drug targets in Leishmania. Recombinant protein for the shortest of these showed negligible peroxidase activity with glutathione as the electron donor indicating that it is not a bone fide glutathione peroxidase. Read More

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November 2007

MIDA1 is a sequence specific DNA binding protein with novel DNA binding properties.

Genes Cells 2000 Sep;5(9):699-709

Department of Cell Biology, Institute of Development, Ageing and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai 980-8575, Japan.

Background: Id proteins not only regulate cell differentiation negatively, but they also promote growth and apoptosis. To know the mechanism of how Id regulates cell fate, we previously isolated an Id-associating protein, MIDA1, which positively regulates cell growth. Its predicted amino acid sequence contains tryptophan-mediated repeats (Tryp-med repeats) similar to the DNA binding region of the c-Myb oncoprotein. Read More

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September 2000

Identification and characterisation of a functional peroxidoxin from Leishmania major.

Mol Biochem Parasitol 1998 Oct;96(1-2):125-37

Department of Medicine, University of Cambridge, Addenbrooke's Hospital, UK.

Leishmania spp. encounter damaging oxygen metabolites from endogenous metabolic processes as well as from exogenous sources, such as inside the gut of the sandfly vector and within host macrophages. The recently described peroxidoxin protein family form part of a novel pathway for metabolising hydrogen peroxide that, in trypanosomatids, links peroxide reduction to NADPH oxidation via trypanothione. Read More

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October 1998

Cloning, expression and reconstitution of the trypanothione-dependent peroxidase system of Crithidia fasciculata.

Mol Biochem Parasitol 1998 Oct;96(1-2):111-23

Department of Biochemistry, University of Dundee, Scotland, UK.

As a consequence of aerobic metabolism, trypanosomatids are exposed to reactive oxygen intermediates such as superoxide, hydrogen peroxide and the hydroxyl radical. Metabolism of hydrogen peroxide in Crithidia fasciculata is accomplished by three distinct proteins, tryparedoxin, tryparedoxin peroxidase and trypanothione reductase, working in concert with the substrates NADPH and trypanothione. Here, we report the cloning and characterisation of the tryparedoxin (TryX) and tryparedoxin peroxidase (TryP) genes from C. Read More

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October 1998

The 42.5 kDa subunit of the NADH: ubiquinone oxidoreductase (complex I) in higher plants is encoded by the mitochondrial nad7 gene.

Mol Gen Genet 1994 Jul;244(1):33-40

Institut für Genbiologische Forschung Berlin, Germany.

The N-terminal amino acid sequence of a 42.5 kDa subunit of the NADH: ubiquinone oxidoreductase (complex I) from potato has been determined by direct protein sequencing. The sequence was found to be homologous to that of the nuclear-encoded 49 kDa complex I subunit of bovine and Neurospora mitochondria and to the sequence deduced from the mitochondrial nad7 gene identified in the mitochondrial (mt) DNA of tryp anosomes and the moss Marchantia. Read More

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Purification and cloning of a novel serine protease, RNK-Tryp-2, from the granules of a rat NK cell leukemia.

J Immunol 1994 Mar;152(5):2289-97

Biological Carcinogenesis and Development Program, PRI/DynCorp, Frederick, MD 21702.

We have biochemically purified a 27-kDa serine protease (designated RNK-Tryp-2) from the granules of the rat large granular lymphocyte leukemia cell line (RNK-16) which has tryptase activity. Utilizing molecular sieve chromatography and reverse-phase HPLC, we purified RNK-Tryp-2 to homogeneity and sequenced 33 NH2-terminal amino acids. Oligonucleotide primers were used in the PCR to generate a 528-bp cDNA clone encoding a novel serine protease from RNK-16 mRNA. Read More

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In vitro formation of an active multienzyme complex in the tryptophan pathway of Neurospora crassa.

Proc Natl Acad Sci U S A 1969 Nov;64(3):1072-8

A multienzyme complex that catalyzes the anthranilate synthetase, phosphoribosylanthranilate (PRA) isomerase, and indoleglycerolphosphate (InGP) synthetase reactions was produced in vitro when extracts from a tryp-1 mutant and a tryp-2 mutant of Neurospora crassa were mixed. The sedimentation values and the molecular weights for the interacting components obtained from the mutants were estimated by sucrose gradients and by gel filtration on Sephadex columns. The component coded for by the tryp-2 gene (a-component) which is present in the tryp-1-17 mutant has a sedimentation coefficient of 4. Read More

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November 1969

Chromosomal relocation of prophage-associated bacterial genes.

J Bacteriol 1966 Apr;91(4):1469-76

Taylor, M. W. (Stanford University, Stanford, Calif. Read More

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