5,582 results match your criteria trpv1 channels

Time-dependent progression of neurogenic lower urinary tract dysfunction after spinal cord injury in the mouse model.

Am J Physiol Renal Physiol 2021 May 10. Epub 2021 May 10.

Department of Urology, University of Pittsburgh, United Statesgrid.21925.3d.

This study evaluated the time-course changes in bladder and external urinary sphincter (EUS) activity as well as the expression of mechanosensitive channels in lumbosacral dorsal root ganglia (DRG) after spinal cord injury (SCI). Female C57BL/6N mice in the SCI group underwent transection of the Th8/9 spinal cord. Spinal intact mice and SCI mice at 2, 4 and 6 weeks post SCI were evaluated by single-filling cystometry and EUS-electromyography (EMG). Read More

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Involvement of TRPV1 and TRPA1 in the modulation of pacemaker potentials in the mouse ileum.

Cell Calcium 2021 Apr 30;97:102417. Epub 2021 Apr 30.

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.

Background: The roles of transient receptor potential cation channel, subfamily V, member 1 (TRPV1) and subfamily A, member 1 (TRPA1) in mechanisms of gastrointestinal motility are complex. This study aimed to clarify the effects of several TRPV1 and TRPA1 ligands on the electrical potentials generated by pacemaker cells in the mouse-isolated ileum.

Method: The pacemaker potentials of ileal segments of mice were recorded extracellularly using a 60-channel microelectrode array. Read More

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Pseudomonas aeruginosa-induced nociceptor activation increases susceptibility to infection.

PLoS Pathog 2021 May 6;17(5):e1009557. Epub 2021 May 6.

Department of Medicine, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.

We report a rapid reduction in blink reflexes during in vivo ocular Pseudomonas aeruginosa infection, which is commonly attributed and indicative of functional neuronal damage. Sensory neurons derived in vitro from trigeminal ganglia (TG) were able to directly respond to P. aeruginosa but reacted significantly less to strains of P. Read More

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Characterization of Ca-Sensing Receptor-Mediated Ca Influx in Microvascular bEND.3 Endothelial Cells.

Chin J Physiol 2021 Mar-Apr;64(2):80-87

Department of Physiology, China Medical University, Taichung, Taiwan.

Ca-sensing receptors (CaSR), activated by elevated concentrations of extracellular Ca, have been known to regulate functions of thyroid cells, neurons, and endothelial cells (EC). In this report, we studied CaSR-mediated Ca influx in mouse cerebral microvascular EC (bEND.3 cells). Read More

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Activation of TRPV1 by Capsaicin or Heat Drives Changes in 2-Acyl Glycerols and -Acyl Ethanolamines in a Time, Dose, and Temperature Dependent Manner.

Front Cell Dev Biol 2021 16;9:611952. Epub 2021 Apr 16.

Bradshaw Laboratory of Lipid Neuroscience, Indiana University Bloomington, Bloomington, IN, United States.

Endocannabinoids (eCBs) and transient receptor potential (TRP) channels are associated with thermoregulation; however, there are many gaps in the understanding of how these signaling systems work together in responding to changes in temperature. TRPV1, a calcium-permeable ion channel, is activated by capsaicin, elevated temperature, the eCB Anandamide, and over 15 additional endogenous lipids. There is also evidence for signaling crosstalk between TRPV1 and the eCB receptor, CB. Read More

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[Involvement of Pirt /TRPV1 signaling in acupuncture-induced reduction of visceral hypersensitivity in diarrhea-predominant irritable bowel syndrome rats].

Zhen Ci Yan Jiu 2021 Apr;46(4):278-83

School of Acupuncture-moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.

Objective: To observe the effect of electroacupuncture (EA) on the expression of Pirt (a regulator of TRPV1) in the dorsal root ganglia (DRG) and transient receptor potential vanilloid 1 (TRPV1, a molecular sensor of noxious heat and capsaicin) in the colonic mucosa in rats suffering from diarrhea-predominant irritable bowel syndrome (IBS-D), so as to explore its mechanisms underlying alleviation of visceral hypersensitivity of IBS-D.

Methods: Forty SD rats (half male and half female) were randomly assigned to control, model, EA, and medication groups by lottery, with 10 rats in each group. The IBS-D model was established by chronic unpredictable mild stress (CUMS) and gavage of senna soaking fluid. Read More

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TRP Channels in Brain Tumors.

Front Cell Dev Biol 2021 13;9:617801. Epub 2021 Apr 13.

CNRS, Inserm, CHU Lille, Centre Oscar Lambret, UMR 9020-UMR 1277-Canther-Cancer Heterogeneity, Plasticity and Resistance to Therapies, University of Lille, Lille, France.

Malignant glioma including glioblastoma (GBM) is the most common group of primary brain tumors. Despite standard optimized treatment consisting of extensive resection followed by radiotherapy/concomitant and adjuvant therapy, GBM remains one of the most aggressive human cancers. GBM is a typical example of intra-heterogeneity modeled by different micro-environmental situations, one of the main causes of resistance to conventional treatments. Read More

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Channelling the Force to Reprogram the Matrix: Mechanosensitive Ion Channels in Cardiac Fibroblasts.

Cells 2021 Apr 23;10(5). Epub 2021 Apr 23.

Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, Leeds LS2 9JT, UK.

Cardiac fibroblasts (CF) play a pivotal role in preserving myocardial function and integrity of the heart tissue after injury, but also contribute to future susceptibility to heart failure. CF sense changes to the cardiac environment through chemical and mechanical cues that trigger changes in cellular function. In recent years, mechanosensitive ion channels have been implicated as key modulators of a range of CF functions that are important to fibrotic cardiac remodelling, including cell proliferation, myofibroblast differentiation, extracellular matrix turnover and paracrine signalling. Read More

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Involvement of Opioid System and TRPM8/TRPA1 Channels in the Antinociceptive Effect of .

Biomolecules 2021 Apr 17;11(4). Epub 2021 Apr 17.

Laboratory of Autoimmunity and Immunopharmacology (LAIF), Department of Health Sciences, Campus Araranguá, Universidade Federal de Santa Catarina, Araranguá 88906-072, Brazil.

is a "super-food" and has attracted researchers' attention due to its anti-inflammatory, antioxidant, and analgesic properties. Herein, we investigated the antinociceptive effects of in different rodent behavior models of inflammatory pain. Male Swiss mice were treated with (3-300 mg/kg, p. Read More

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Hypersensitivity Induced by Intrathecal Bradykinin Administration Is Enhanced by N-oleoyldopamine (OLDA) and Prevented by TRPV1 Antagonist.

Int J Mol Sci 2021 Apr 2;22(7). Epub 2021 Apr 2.

Laboratory of Pain Research, Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 14220 Prague, Czech Republic.

Transient receptor potential vanilloid 1 (TRPV1) channels contribute to the development of several chronic pain states and represent a possible therapeutic target in many painful disease treatment. Proinflammatory mediator bradykinin (BK) sensitizes TRPV1, whereas noxious peripheral stimulation increases BK level in the spinal cord. Here, we investigated the involvement of spinal TRPV1 in thermal and mechanical hypersensitivity, evoked by intrathecal (i. Read More

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An operant temperature sensory assay provides a means to assess thermal discrimination.

Mol Pain 2021 Jan-Dec;17:17448069211013633

Molecular Genetics Section, National Institute of Dental and Craniofacial Research/NIH, Bethesda, MD, USA.

Mouse behavioral assays have proven useful for the study of thermosensation, helping to identify receptors and circuits responsible for the transduction of thermal stimuli and information relay to the brain. However, these methods typically rely on observation of behavioral responses to various temperature stimuli to infer sensory ability and are often unable to disambiguate innocuous thermosensation from thermal nociception or to study thermosensory circuitry which do not produce easily detectable innate behavioral responses. Here we demonstrate a new testing apparatus capable of delivering small, rapid temperature change stimuli to the mouse's skin, permitting the use of operant conditioning to train mice to recognize and report temperature change. Read More

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7-Hydroxy Frullanolide, a sesquiterpene lactone, increases intracellular calcium amounts, lowers CD4 T cell and macrophage responses, and ameliorates DSS-induced colitis.

Int Immunopharmacol 2021 Apr 24;97:107655. Epub 2021 Apr 24.

Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India. Electronic address:

Sesquiterpene lactones are a class of anti-inflammatory molecules obtained from plants belonging to the Asteraceae family. In this study, the effects of 7-hydroxy frullanolide (7HF), a sesquiterpene lactone, in inhibiting CD4 T cell and peritoneal macrophage responses were investigated. 7HF, in a dose dependent manner, lowers CD69 upregulation, IL2 production and CD4 T cell cycling upon activation with the combination of anti-CD3 and anti-CD28. Read More

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The Calcium-Activated Chloride Channel TMEM16A is Inhibitied by Liquiritigenin.

Front Pharmacol 2021 8;12:628968. Epub 2021 Apr 8.

Department of Physiology, Showa University School of Medicine, Tokyo, Japan.

The transmembrane 16 (TMEM16) family contains 10 subtypes, and the function of each protein is different. TMEM16A is a calcium-activated chloride channel involved in physiological and pathological situations. Liquiritigenin is an aglycone derived from , and it is generated via the metabolism of enterobacterial flora. Read More

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Activation of V vasopressin receptors excite subicular pyramidal neurons by activating TRPV1 and depressing GIRK channels.

Neuropharmacology 2021 Apr 20;190:108565. Epub 2021 Apr 20.

Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, 58203, USA.

Arginine vasopressin (AVP) is a nonapeptide that serves as a neuromodulator in the brain and a hormone in the periphery that regulates water homeostasis and vasoconstriction. The subiculum is the major output region of the hippocampus and an integral component in the networks that processes sensory and motor cues to form a cognitive map encoding spatial, contextual, and emotional information. Whereas the subiculum expresses high densities of AVP-binding sites and AVP has been shown to increase the synaptic excitability of subicular pyramidal neurons, the underlying cellular and molecular mechanisms have not been determined. Read More

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Identification of herbal components as TRPA1 agonists and TRPM8 antagonists.

J Nat Med 2021 Apr 20. Epub 2021 Apr 20.

Tsumura Kampo Research Laboratories, Kampo Research and Development Division, Tsumura & Co, 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki, 300-1192, Japan.

Transient receptor potential (TRP) channels are non-selective cation channels that are implicated in analgesia, bowel motility, wound healing, thermoregulation, vasodilation and voiding dysfunction. Many natural products have been reported to affect the activity of TRP channels. We hypothesize that numerous traditional herbal medicines (THMs) might exert their pharmacological activity through modulating the activity of TRP channels. Read More

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Local Anesthetics Inhibit Transient Receptor Potential Vanilloid Subtype 3 Channel Function in Xenopus Oocytes.

Anesth Analg 2021 Apr 15. Epub 2021 Apr 15.

From the Department of Anesthesiology, School of Medicine.

Background: The transient receptor potential vanilloid subtype 3 (TRPV3) channel is activated by innocuous temperature and several chemical stimuli. It is proposed to be involved in pathological pain development and is therefore considered a potential target for treating pain. Local anesthetics have been used for patients with both acute and chronic pain. Read More

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Striatal TRPV1 activation by acetaminophen ameliorates dopamine D2 receptor antagonists-induced orofacial dyskinesia.

JCI Insight 2021 Apr 15. Epub 2021 Apr 15.

Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.

Antipsychotics often cause tardive dyskinesia, an adverse symptom of involuntary hyperkinetic movements. Analysis of the U.S. Read More

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Petasin and isopetasin reduce CGRP release from trigeminal afferents indicating an inhibitory effect on TRPA1 and TRPV1 receptor channels.

J Headache Pain 2021 Apr 13;22(1):23. Epub 2021 Apr 13.

Institute of Physiology and Pathophysiology, Friedrich-Alexander-University of Erlangen-Nürnberg, Universitätsstraße 17, 91054, Erlangen, Germany.

Background: Butterbur root extract with its active ingredients petasin and isopetasin has been used in the prophylactic treatment of migraine for years, while its sites of action are not completely clear. Calcitonin gene-related peptide (CGRP) is known as a biomarker and promoting factor of migraine. We set out to investigate the impact of petasins on the CGRP release from trigeminal afferents induced by activation of the calcium conducting transient receptor potential channels (TRPs) of the subtypes TRPA1 and TRPV1. Read More

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Extracellular cap domain is an essential component of the TRPV1 gating mechanism.

Nat Commun 2021 04 12;12(1):2154. Epub 2021 Apr 12.

Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.

Transient receptor potential (TRP) channels are polymodal molecular sensors involved in numerous physiological processes and implicated in a variety of human diseases. Several structures of the founding member of the TRP channel family, TRPV1, are available, all of which were determined for the protein missing the N- and C-termini and the extracellular S5-P-loop. Here, we present structures of the full-length thirteen-lined ground squirrel TRPV1 solved by cryo-EM. Read More

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Deciphering the synergistic network regulation of active components from SiNiSan against irritable bowel syndrome via a comprehensive strategy: Combined effects of synephrine, paeoniflorin and naringin.

Phytomedicine 2021 Jun 24;86:153527. Epub 2021 Feb 24.

College of Life Sciences, Nanjing Normal University, Nanjing 210023, China. Electronic address:

Background: SiNiSan (SNS) is an ancient Chinese herbal prescription, and the current clinical treatment of irritable bowel syndrome (IBS) is effective. In the previous study of the research team, the multi-functional co-synergism of SNS against IBS was presented. Some potential drug targets and candidate ligands were predicted. Read More

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The diversity in sensitivity of TRPA1 and TRPV1 of various animals to polyphenols.

Biomed Res 2021 ;42(2):43-51

Department of Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology.

The perception of tastes is sensed by the receptors that stimulate sensory cells. We previously reported that TRPA1 and TRPV1 channels expressed in the oral cavity of mammals, are activated by the auto-oxidized product of epigallocatechin gallate (oxiEGCG), a major astringent catechin in green tea. Here, we investigated and compared the sensitivity of TRPA1 and TRPV1 from various animals to astringent polyphenols. Read More

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January 2021

TRPV1 channels as a newly identified target for vitamin D.

Channels (Austin) 2021 Dec;15(1):360-374

Department of Pharmacology and the Alberta Diabetes Institute, University of Alberta, Edmonton, Canada.

Vitamin D is known to elicit many biological effects in diverse tissue types and is thought to act almost exclusively upon its canonical receptor within the nucleus, leading to gene transcriptional changes and the subsequent cellular response. However, not all the observed effects of vitamin D can be attributed to this sole mechanism, and other cellular targets likely exist but remain to be identified. Our recent discovery that vitamin D is a partial agonist of the Transient Receptor Potential Vanilloid family 1 (TRPV1) channel may provide new insights as to how this important vitamin exerts its biological effects either independently or in addition to the nuclear vitamin D receptor. Read More

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December 2021

Icariin ameliorates partial sciatic nerve ligation induced neuropathic pain in rats: an evidence of in silico and in vivo studies.

J Pharm Pharmacol 2021 Apr 3. Epub 2021 Apr 3.

Division of Pharmacology, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Visvavidyalayam, Tirupati, Andhra Pradesh, India.

Objectives: Neuropathic pain (NP) is a chronic inflammation of the sciatic nerve, associated with complex pathophysiological events like neuronal ectopic discharge with changes in neurotransmitters, growth factors, receptors/ion channels including N-methyl-d-aspartate receptors, Transient receptor cation channels, Voltage-gated calcium channels. All these events eventually lead to inflammation and apoptosis of the sciatic nerve in NP. Icariin (ICA), a natural flavonoid is well known for its anti-inflammatory potential. Read More

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Synthesis, Anticonvulsant, and Antinociceptive Activity of New 3-(2-Chlorophenyl)- and 3-(3-Chlorophenyl)-2,5-dioxo-pyrrolidin-1-yl-acetamides.

Molecules 2021 Mar 12;26(6). Epub 2021 Mar 12.

Department of Medicinal Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9 St., 30-688 Krakow, Poland.

The new series of 3-(2-chlorophenyl)- and 3-(3-chlorophenyl)-pyrrolidine-2,5-dione-acetamide derivatives as potential anticonvulsant and analgesic agents was synthesized. The compounds obtained were evaluated in the following acute models of epilepsy: maximal electroshock (MES), psychomotor (6 Hz, 32 mA), and subcutaneous pentylenetetrazole (PTZ) seizure tests. The most active substance-3-(2-chlorophenyl)-1-{2-[4-(4-fluorophenyl)piperazin-1-yl]-2-oxoethyl}-pyrrolidine-2,5-dione () showed more beneficial ED and protective index values than the reference drug-valproic acid (68. Read More

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Ca Signalling Induced by NGF Identifies a Subset of Capsaicin-Excitable Neurons Displaying Enhanced Chemo-Nociception in Dorsal Root Ganglion Explants from Adult Mouse.

Int J Mol Sci 2021 Mar 4;22(5). Epub 2021 Mar 4.

International Centre for Neurotherapeutics, Dublin City University, Collins Avenue, D09 V209 Dublin, Ireland.

Nociceptors sense hazards via plasmalemmal cation channels, including transient receptor potential vanilloid 1 (TRPV1). Nerve growth factor (NGF) sensitises TRPV1 to capsaicin (CAPS), modulates nociceptor excitability and induces thermal hyperalgesia, but cellular mechanisms remain unclear. Confocal microscopy was used to image changes in intracellular Ca concentration ([Ca]) across neuronal populations in dorsal root ganglia (DRG) explants from adult mice, which express a fluorescent reporter in their sensory neurons. Read More

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Interactions between Chemesthesis and Taste: Role of TRPA1 and TRPV1.

Int J Mol Sci 2021 Mar 25;22(7). Epub 2021 Mar 25.

Department of Physiology and Biophysics, Virginia Commonwealth University, Richmond, VA 23298, USA.

In addition to the sense of taste and olfaction, chemesthesis, the sensation of irritation, pungency, cooling, warmth, or burning elicited by spices and herbs, plays a central role in food consumption. Many plant-derived molecules demonstrate their chemesthetic properties via the opening of transient receptor potential ankyrin 1 (TRPA1) and transient receptor potential vanilloid 1 (TRPV1) channels. TRPA1 and TRPV1 are structurally related thermosensitive cation channels and are often co-expressed in sensory nerve endings. Read More

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Functional Expression of TRPV1 Ion Channel in the Canine Peripheral Blood Mononuclear Cells.

Int J Mol Sci 2021 Mar 20;22(6). Epub 2021 Mar 20.

Department of Physics and Biophysics, Institute of Biology, Warsaw University of Life Sciences, 02-776 Warsaw, Poland.

TRPV1, known as a capsaicin receptor, is the best-described transient receptor potential (TRP) ion channel. Recently, it was shown to be expressed by non-excitable cells such as lymphocytes. However, the data regarding the functional expression of the TRPV1 channel in the immune cells are often contradictory. Read More

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Remedia Sternutatoria over the Centuries: TRP Mediation.

Molecules 2021 Mar 15;26(6). Epub 2021 Mar 15.

Department of Pharmacology, College of Medicine and Health Sciences, Khalifa University of Science and Technology, Abu Dhabi 127788, United Arab Emirates.

Sneezing (sternutatio) is a poorly understood polysynaptic physiologic reflex phenomenon. Sneezing has exerted a strange fascination on humans throughout history, and induced sneezing was widely used by physicians for therapeutic purposes, on the assumption that sneezing eliminates noxious factors from the body, mainly from the head. The present contribution examines the various mixtures used for inducing sneezes (remedia sternutatoria) over the centuries. Read More

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Sperm Cholesterol Content Modifies Sperm Function and TRPV1-Mediated Sperm Migration.

Int J Mol Sci 2021 Mar 18;22(6). Epub 2021 Mar 18.

Unit of Andrology and Reproduction Medicine, Department of Medicine, University of Padova, 35128 Padova, Italy.

Transient receptor potential channels-vanilloid receptor 1 (TRPV1) regulates thermotaxis in sperm-oriented motility. We investigated the role of membrane cholesterol (Chol) on TRPV1-mediated human sperm migration. Semen samples were obtained from five normozoospemic healthy volunteers. Read More

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