8 results match your criteria trpc4 detect

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Modified Liu-Jun-Zi decoction alleviates visceral hypersensitivity in functional dyspepsia by regulating EC cell-5HT3r signaling in duodenum.

J Ethnopharmacol 2020 Mar 10;250:112468. Epub 2019 Dec 10.

Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, China. Electronic address:

Ethnopharmacological Relevance: Modified Liu-Jun-Zi (MLJZ) is derived from one of the most famous traditional Chinese prescription Liu-Jun-Zi. It exhibits therapeutic effects in functional dyspepsia (FD), but the underlying mechanisms remain not well understood. Enterochromaffin (EC) cells contribute to the pathogeneses of visceral hypersensitivity in functional gastrointestinal disorders. Read More

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Genome-wide association study provides insights into the genetic architecture of bone size and mass in chickens.

Genome 2020 Mar 3;63(3):133-143. Epub 2019 Dec 3.

Jiangsu Institute of Poultry Science, Key Laboratory for Poultry Genetics and Breeding of Jiangsu province, Yangzhou, Jiangsu, 225125, China.

Bone size is an important trait for chickens because of its association with osteoporosis in layers and meat production in broilers. Here, we employed high density genotyping platforms to detect candidate genes for bone traits. Estimates of the narrow heritabilities ranged from 0. Read More

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Calcium permeability of transient receptor potential canonical (TRPC) 4 channels measured by TRPC4-GCaMP6s.

Korean J Physiol Pharmacol 2017 Jan 21;21(1):133-140. Epub 2016 Dec 21.

Department of Physiology, Seoul National University College of Medicine, Seoul 03080, Korea.

Conflicting evidence has been obtained regarding whether transient receptor potential cation channels (TRPC) are store-operated channels (SOCs) or receptor-operated channels (ROCs). Moreover, the Ca/Na permeability ratio differs depending on whether the current-voltage (I-V) curve has a doubly rectifying shape or inward rectifying shape. To investigate the calcium permeability of TRPC4 channels, we attached GCaMP6s to TRPC4 and simultaneously measured the current and calcium signals. Read More

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January 2017

High expression of calcium channel subtypes in uterine fibroid of patients.

Int J Clin Exp Med 2014 15;7(5):1324-30. Epub 2014 May 15.

Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University & Shandong Provincial Key Laboratory of Reproductive Medicine 324 Jingwu Road, Jinan 250021, China.

Aim: To investigate the expression of calcium channel protein in uterine fibroids, and to explore the relationship between calcium signaling pathway and the pathogenesis of uterine fibroids.

Methods: Uterine fibroid tissues (UFC) and adjacent healthy uterine smooth muscle tissues (SMC) were collected from 30 cases of uterine fibroids. Real-time quantitative PCR and western blot were used to detect cell membrane calcium channel protein subtypes: TRPC1, TRPC3, TRPC4, TRPC6, TRPM6 and TRPM7. Read More

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The effects of Nigella sativa (Ns), Anthemis hyalina (Ah) and Citrus sinensis (Cs) extracts on the replication of coronavirus and the expression of TRP genes family.

Mol Biol Rep 2014 Mar 12;41(3):1703-11. Epub 2014 Jan 12.

Department of Medical Biology, Faculty of Medicine, University of Gaziantep, ┼×ehitkamil, 27310, Gaziantep, Turkey,

Extracts of Anthemis hyalina (Ah), Nigella sativa (Ns) and peels of Citrus sinensis (Cs) have been used as folk medicine to fight antimicrobial diseases. To evaluate the effect of extracts of Ah, Ns and Cs on the replication of coronavirus (CoV) and on the expression of TRP genes during coronavirus infection, HeLa-CEACAM1a (HeLa-epithelial carcinoembryonic antigen-related cell adhesion molecule 1a) cells were inoculated with MHV-A59 (mouse hepatitis virus-A59) at moi of 30. 1/50 dilution of the extracts was found to be the safe active dose. Read More

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Expression and distribution of transient receptor potential (TRP) channels in bladder epithelium.

Am J Physiol Renal Physiol 2011 Jan 13;300(1):F49-59. Epub 2010 Oct 13.

Department of Medicine, Renal-Electrolyte Division, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.

The urothelium is proposed to be a sensory tissue that responds to mechanical stress by undergoing dynamic membrane trafficking and neurotransmitter release; however, the molecular basis of this function is poorly understood. Transient receptor potential (TRP) channels are ideal candidates to fulfill such a role as they can sense changes in temperature, osmolarity, and mechanical stimuli, and several are reported to be expressed in the bladder epithelium. However, their complete expression profile is unknown and their cellular localization is largely undefined. Read More

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January 2011

[Expression of transient receptor potential canonical channel proteins in human non-small cell lung cancer].

Zhongguo Fei Ai Za Zhi 2010 Jun;13(6):612-6

State Key Lab of Respiratory Diseases, the first affiliated hospital of Guangzhou Medical College, Guangzhou Medical College, Guangzhou 510120, China.

Background And Objective: Transient receptor potential canonical (TRPC) proteins, a group of Ca2' permeable nonselective cation channels, are thought to constitute store-operated calcium channels (SOCC) and mediate store-operated calcium entry (SOCE) in various cell types. Members of TRPC have been found to be involved in abnormal proliferation, differentiation, and growth of cancer cells. The aim of this study is to detect the mRNA and protein expression of TRPC in non-small cell lung cancer (NSCLC). Read More

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Expression and functional evaluation of transient receptor potential channel 4 in bovine corneal endothelial cells.

Exp Eye Res 2005 Jul;81(1):5-14

School of Optometry, Indiana University, 800 E Atwater Avenue, Bloomington, IN 47405-3680, USA.

We previously found that activation of purinergic receptors mobilizes Ca2+ and enhances bicarbonate transport in bovine corneal endothelial cells (BCEC). Since transient receptor potential channel 4 (TRPC) has been reported to be a candidate for capacitative calcium entry (CCE) and receptor operated calcium entry (ROC), we examined the expression of TRPC4 and evaluated the potential involvement of TRPC4 in CCE or ROC in BCEC. The C-terminus of TRPC4 was fused into the glutathione S-transferase (GST) expression vector. Read More

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