72 results match your criteria tropica investigate


Chemical Biology Tools To Investigate Malaria Parasites.

Chembiochem 2021 Feb 11. Epub 2021 Feb 11.

Centre for Infectious Diseases, Parasitology, Heidelberg University Hospital, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany.

Parasitic diseases like malaria tropica have been shaping human evolution and history since the beginning of mankind. After infection, the response of the human host ranges from asymptomatic to severe and may culminate in death. Therefore, proper examination of the parasite's biology is pivotal to deciphering unique molecular, biochemical and cell biological processes, which in turn ensure the identification of treatment strategies, such as potent drug targets and vaccine candidates. Read More

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February 2021

The leishmanicidal effect of Lucilia sericata larval saliva and hemolymph on in vitro Leishmania tropica.

Parasit Vectors 2021 Jan 11;14(1):40. Epub 2021 Jan 11.

Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: Leishmaniasis is a major parasitic disease worldwide, except in Australia and Antarctica, and it poses a significant public health problem. Due to the absence of safe and effective vaccines and drugs, researchers have begun an extensive search for new drugs. The aim of the current study was to investigate the in vitro leishmanicidal activity of larval saliva and hemolymph of Lucilia sericata on Leishmania tropica. Read More

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January 2021

Investigation of Phlebotominae (Diptera: Psychodidae) Fauna, Seasonal Dynamics, and Natural Leishmania spp. Infection in Muğla, Southwest of Turkey.

Acta Trop 2021 Apr 9;216:105827. Epub 2021 Jan 9.

Department of Parasitology, Faculty of Medicine, Ege University, Izmir, Turkey.

Due to its geographical location, Muğla province is one of the most frequently used places by refugees. Although leishmaniasis have been previously reported in this region, there is a lack of information on the etiological agent and possible vectors. The main objectives of this study were; i) to investigate the sand fly fauna, ii) to reveal the natural Leishmania spp. Read More

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Evaluation of the effect of extracts on the in vitro viability of promastigotes in comparison to Glucantime.

J Parasit Dis 2020 Dec 27;44(4):858-863. Epub 2020 Sep 27.

Department of Pharmacognosy, Damascus University, Damascus, Syria.

Cutaneous leishmaniasis is a skin disease that pretends with skin lesions, mainly ulcers, on exposed parts of the body. It's caused mainly by parasites belonging to the genus , such as Medicinal plants, which have sparked recent researches attention, consider one of the richest sources of active compounds against the parasite including . This study was conducted to investigate the antileishmanial effect of different extracts of grown in Syria, particularly the methanolic extract of seeds and roots as well as its alkaloid fractions on promastigotes growth in vitro. Read More

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December 2020

Presence and diversity of Leishmania RNA virus in an old zoonotic cutaneous leishmaniasis focus, northeastern Iran: haplotype and phylogenetic based approach.

Int J Infect Dis 2020 Dec 15;101:6-13. Epub 2020 Sep 15.

Toxoplasmosis Research Center, Communicable Diseases Institute, Iranian National Registry Center for Lophomoniasis and Toxoplasmosis, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Objective: Leishmania RNA virus (LRV) is a double-stranded RNA (dsRNA) virus that circulates within many species of the Leishmania parasite. In this study, we aimed to investigate the presence of LRV2 circulating in Leishmania isolates in an old focus of ZCL located in northeastern of Iran.

Methods: Leishmania isolates were collected from 85 patients that confirmed to have cutaneous leishmaniasis (CL) based on parasitological examination. Read More

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December 2020

Phylogenetic position of Leishmania tropica isolates from an old endemic focus in south-eastern Iran; relying on atypical cutaneous leishmaniasis.

Transbound Emerg Dis 2020 Sep 3. Epub 2020 Sep 3.

Leishmaniasis Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran.

Cutaneous leishmaniasis (CL) is a major health problem in Iran, with a heavy burden on human health and society. There is little knowledge about the molecular epidemiology of the disease, as well as phylogenetic relationship of causative agents in south-eastern Iran. The aim of the present study was to investigate the molecular aspects of CL, especially atypical CL in the Bam district, Kerman province, south-eastern Iran, as an endemic region of CL in Iran. Read More

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September 2020

[Investigation of Anti-leishmanial Effects of Bee Products (Honey, Propolis) on Leishmania tropica Promastigotes].

Mikrobiyol Bul 2020 Jul;54(3):479-489

Inonü University Faculty of Medicine, Department of Medical Parasitology, Malatya, Turkey.

This study was aimed to investigate the anti-leishmanial effects of bee products (honey and propolis) by using the causative agent of cutaneous leishmaniasis Leishmania tropica promastigotes, in in vitro culture. In vitro anti-leishmanial efficacy of honey (pine, flower and chestnut) and propolis used in the study were evaluated using the microdilution method. Honey, which is a bee product, was dissolved with RPMI medium containing fetal calf serum (FCS) and diluted in the same medium, and serial dilutions were prepared in concentrations between 62. Read More

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[Diversity of Leishmania Strains Isolated from Cutaneous Leishmaniasis Patients in Turkey and its Reflection to Clinics in Mice Model].

Mikrobiyol Bul 2020 Jul;54(3):429-443

Ege University Faculty of Medicine, Department of Medical Biology, Izmir, Turkey.

Although asexual reproduction has been attributed to Leishmania species, genetic exchange has recently been demonstrated, which helped emerging of hybrid isolates. Situated on the crossroads between three continents, Leishmania hybrids may be present in Turkey. In Turkey, visceral leishmaniasis caused by Leishmania infantum is less common, while cutaneous leishmaniasis (CL) caused by Leishmania tropica and L. Read More

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Cutaneous leishmaniasis in male schoolchildren in the upper and lower Dir districts of Khyber Pakhtunkhwa, and a review of previous record in Pakistan.

Acta Trop 2020 Sep 10;209:105578. Epub 2020 Jun 10.

Institute of Environmental and Occupational Health Sciences, Department of Public Health, College of Public Health, National Taiwan University, Taipei, Taiwan.

Documented reports are limited, showing cutaneous leishmaniasis (CL) as a severe threat to schoolchildren in Pakistan. The present study aimed to investigate the clinico-epidemiology and associated risk factors of CL in local and Afghan male schoolchildren between 6 and 16 years of age. The experimental strategy involved a questionnaire for the collection of information and clinical diagnosis (microscopy and semi-nested PCR) of 113 CL symptomatic schoolchildren out of 8,833 schoolchildren (7,175 local and 1,658 Afghan refugees) studying in nineteen schools of the Upper and Lower Dir Districts, Khyber Pakhtunkhwa, Pakistan. Read More

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September 2020

First molecular detection and identification of species in small wild rodents from Turkey.

Parasitology 2020 09 14;147(10):1088-1093. Epub 2020 May 14.

Ege University, Faculty of Medicine, Department of Parasitology, Izmir, Turkey.

Leishmaniasis is a parasitic disease infecting animals and humans. Two clinical forms (Visceral and cutaneous leishmaniasis) and four species are reported to be present in Turkey. Several studies have investigated canine and human leishmaniasis in Turkey but no study was performed to screen the infection among wild rodents, so far. Read More

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September 2020

Camel nanobodies: Promising molecular tools against leishmaniasis.

Parasite Immunol 2020 09 14;42(9):e12718. Epub 2020 Jul 14.

Division of Molecular Biomedicine, Department of Molecular Biology and Biotechnology, AECS, Damascus, Syria.

Aim: To characterize several anti-Leishmania tropica nanobodies and to investigate their effect on Leishmania infection.

Methods: Several immunological tests were implied to characterize five different (as confirmed by sequencing) anti-L tropica nanobodies (NbLt05, NbLt06, NbLt14, NbLt24 and NbLt36) against parasite lysates or intact cells from different stages, promastigotes and amastigotes. Direct inhibitory effect of these nanobodies on parasite infection cycle on macrophages was tested in cell culture. Read More

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September 2020

Reactive oxygen species generating photosynthesized ferromagnetic iron oxide nanorods as promising antileishmanial agent.

Nanomedicine (Lond) 2020 04 20;15(8):755-771. Epub 2020 Mar 20.

Institute of Integrative Biosciences, CECOS University of IT & Emerging Sciences, Peshawar, Pakistan.

To investigate the photodynamic therapeutic potential of ferromagnetic iron oxide nanorods (FIONs), using as a reducing agent, against . FIONs were characterized using ultraviolet visible spectroscopy, x-ray diffraction and scanning electron microscopy. FIONs showed excellent activity against promastigotes and amastigotes (IC 0. Read More

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Serum 25-hydroxyvitamin D level and vitamin D receptor (VDR) polymorphisms in patients infected with : a case control study.

J Parasit Dis 2020 Mar 26;44(1):40-48. Epub 2019 Sep 26.

1Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.

, an obligate intracellular parasite is eliminated by a strong Th-1 host response. As Vitamin D metabolism and its receptor activity are important factors in human native immune system against some microorganisms, we hypothesized that VDR gene polymorphisms and concentration of Vitamin D might have effect on incidence of cutaneous leishmaniasis. The aim of this study was to investigate the association between VDR gene polymorphism and/or the serum vitamin D level and leishmaniasis in the infected patients in comparison to the healthy individuals. Read More

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Facile green synthesis approach for the production of chromium oxide nanoparticles and their different in vitro biological activities.

Microsc Res Tech 2020 Jun 14;83(6):706-719. Epub 2020 Mar 14.

Department of Plant Sciences, Quaid-i-Azam University Islamabad, Islamabad, Pakistan.

Green synthesis of nanoparticles using plants has become a promising substitute for the conventional chemical synthesis methods. In the present study, our aim was to synthesize chromium oxide nanoparticles (Cr O NPs) through a facile, low-cost, eco-friendly route using leaf extract of Rhamnus virgata (RV). The formation of Cr O NPs was confirmed and characterized by spectroscopic profile of UV-Vis, EDX, FTIR, and XRD analyses. Read More

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A quantitative proteomic and bioinformatics analysis of proteins in metacyclogenesis of Leishmania tropica.

Acta Trop 2020 Feb 21;202:105227. Epub 2019 Oct 21.

Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Recently there has a growing interest in MS-based analysis on Leishmania for biology study, host-parasite interaction and drug target discovery. The aims of this study were to analyzed protein profiles in the procyclic and metacyclic stages of L. tropica, and investigate their potential role in metacyclogenesis molecular mechanisms. Read More

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February 2020

Differences in tail feather growth rate in storm-petrels breeding in the Northern and Southern hemisphere: a ptilochronological approach.

PeerJ 2019 15;7:e7807. Epub 2019 Oct 15.

Department of Vertebrate Ecology and Zoology, Faculty of Biology, University of Gdańsk, Gdańsk, Poland.

Moulting and breeding are costly stages in the avian annual cycle and may impose trade-offs in energy allocation between both stages or in their timing. Here, we compared feather growth rates (FGR) of rectrices in adults between two pairs of small pelagic Procellariiformes species differing in moult-breeding strategies: the European storm-petrel and Leach's storm-petrel breeding in the Northern Hemisphere (Faroe Islands), showing moult-breeding overlap in tail feathers; and the Wilson's storm-petrel and black-bellied storm-petrel , breeding in the Southern Hemisphere (South Shetlands), temporally separating moult and breeding. We used ptilochronology (i. Read More

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October 2019

Leishmanicidal effects of amphotericin B in combination with selenium loaded on niosome against .

J Parasit Dis 2019 Jun 1;43(2):176-185. Epub 2019 Jan 1.

4HIV/STI Surveillance Research Center, and WHO Collaborating Center for HIV Surveillance, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran.

The strategy for improving the treatment of leishmaniasis by the World Health Organization, is the development of new drugs and combination therapy. The aim of this survey was to investigate the effect of amphotericin B (AmB) in combination with selenium, in a simple or niosomal form, on () by in vitro advanced assays. In this study, a niosomal formulation of AmB with selenium was prepared and characterized based on size and morphology. Read More

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[Transfection of Leishmania tropica with green fluorescent protein (gfp) gene and investigation of the in vitro drug effect].

Mikrobiyol Bul 2019 Apr;53(2):213-223

Rosalind Franklind University, Chicago Medical School, Department of Microbiology and Immunology, Illinois, USA.

Cutaneous leishmaniasis (CL) is a parasitic disease transmitted by vector sand flies Phlebotomus and Lutzomyia. This disease is characterized by long time non-healing skin lesions, and caused by Leishmania species. CL is the most common infection in Eastern and Southeastern Anatolia in Turkey and L. Read More

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Detection of Leishmania RNA virus 2 in Leishmania species from Turkey.

Trans R Soc Trop Med Hyg 2019 07;113(7):410-417

Ege University, Medical Faculty, Department of Parasitology, Bornova, İzmir, Turkey.

Background: Leishmania RNA virus (LRV) is a double-stranded RNA (dsRNA) virus infecting some Leishmania strains and triggering a destructive hyperinflammatory response in mammalian hosts in the New World. There is limited knowledge of the presence of this virus in Old World Leishmania species and its role in the outcome of the disease. We aimed to investigate the presence of LRV in Leishmania species/strains from Turkey. Read More

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Prevalence of Leishmania species among patients with cutaneous leishmaniasis in Qassim province of Saudi Arabia.

BMC Public Health 2019 Apr 5;19(1):384. Epub 2019 Apr 5.

Department of Dermatology, College of Medicine, Qassim University, Buraidah, Saudi Arabia.

Background: Leishmaniasis is a parasitic infection endemic in more than ninety countries of the world. The cutaneous leishmaniasis (CL) is a most common form of leishmaniasis and it remains to be a major public health issue in Saudi Arabia. This study was undertaken to investigate the Leishmania species responsible for CL infection in different provinces of Qassim, Saudi Arabia. Read More

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Gene expression analysis of antimony resistance in Leishmania tropica using quantitative real-time PCR focused on genes involved in trypanothione metabolism and drug transport.

Arch Dermatol Res 2019 Jan 2;311(1):9-17. Epub 2018 Nov 2.

Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Pentavalent antimonials remain the treatment of choice for all the clinical forms of leishmaniasis. The increasing rates of antimony resistance are becoming a serious health problem in treatment of anthroponotic cutaneous leishmaniasis (ACL). Accordingly, unraveling molecular markers is crucial for improving medication strategies and monitoring of drug-resistant parasites. Read More

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January 2019

[Do the rodents have a role in transmission of cutaneous leishmaniasis in Turkey?]

Mikrobiyol Bul 2018 Jul;52(3):259-272

Ege University Faculty of Medicine, Department of Medical Biology, Izmir, Turkey.

Leishmaniasis is a zoonotic/anthroponotic vector borne parasitic infection which is caused by Leishmania species and transmitted by sand flies (Phlebotomus spp.) The reservoirs of Leishmania species in nature are various wild and domestic carnivores, rodents and human. The aim of this study was to investigate whether the rodents in genera Meriones, Mesocricetus, Rattus and Mus which inhabit in the natural habitat of our country could be natural reservoirs of Leishmania tropica, Leishmania infantum, Leishmania major and Leishmania donovani for cutaneous Leishmaniasis (CL). Read More

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Using focused pharmacovigilance for ensuring patient safety against antileishmanial drugs in Bangladesh's National Kala-azar Elimination Programme.

Infect Dis Poverty 2018 Aug 13;7(1):80. Epub 2018 Aug 13.

68 Shaheed Taj Uddin Ahmed Sarani, Parasitology Laboratory, International Centre for Diarrhoeal Disease Research (icddr,b), Mohakhali, Dhaka, 1212, Bangladesh.

Background: Adverse effects of antileishmanial drugs can affect patients' quality of life and adherence to therapy for visceral leishmaniasis (VL) and post-kala-azar dermal leishmaniasis (PKDL). In Bangladesh, there are 26 treatment centers that manage leishmaniasis cases coming from 100 endemic upazilas (subdistricts) of 26 districts (these include VL, PKDL, treatment failure, and relapse VL and cutaneous leishmaniasis cases). This study aimed to investigate the feasibility of using focused pharmacovigilance for VL (VLPV) in Bangladesh's National Kala-azar Elimination Programme for the early detection and prevention of expected and unexpected adverse drug reactions (ADRs). Read More

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Synergistic degradation of crude oil by indigenous bacterial consortium and exogenous fungus Scedosporium boydii.

Bioresour Technol 2018 Sep 19;264:190-197. Epub 2018 May 19.

Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China.

The purpose of this study was to investigate the potential of defined co-culture of indigenous bacterial consortium and exogenous fungus Scedosporium boydii for biodegradation of crude oil. After 7 days of incubation, residual oil, n-alkanes and aromatic fraction were analyzed. The degradation rate of crude oil was increased from 61. Read More

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September 2018

Evaluation of In vitro and In vivo Drug Efficacy Over Leishmania tropica: A Pilot Study.

Turkiye Parazitol Derg 2018 Mar;42(1):11-19

Adnan Menderes Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye.

Objective: Two pentavalent antimonials, meglumine antimoniate (Glucantime®, France) and sodium stibogluconate (Pentostam®, England), are used to treat cutaneous leishmaniasis (CL) in Turkey. The present study, serving as a guidebook for young researchers, aims to provide basis for conducting drug resistance tests and active ingredient scanning in in vitro and in vivo models.

Methods: A CL isolate kept in liquid nitrogen was initially thawed and genotyped by real-time polymerase chain reaction (PCR) using ITS1 prob. Read More

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Cutaneous Leishmaniasis among Syrian Refugees in Jordan: a Retrospective Study.

Bull Soc Pathol Exot 2018 ;111(5):295-300

Department of Biology, Jordan University of Science and Technology, P. O. Box 3030, Irbid, Jordan.

The aim of this study is to investigate cutaneous leishmaniasis (CL) among Syrian refugees residing in Jordan. A total of 558 Syrian refugee patients were clinically diagnosed with cutaneous leishmaniasis during 2010-2016. For each patient, sex, age, location, and number of lesions were recorded. Read More

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Polarity based characterization of biologically active extracts of Ajuga bracteosa Wall. ex Benth. and RP-HPLC analysis.

BMC Complement Altern Med 2017 Sep 5;17(1):443. Epub 2017 Sep 5.

Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.

Background: The concept of botanical therapeutics has revitalized due to wide importance of plant derived pharmaceuticals. Therefore, the ameliorative characteristics of Ajuga bracteosa were studied.

Methods: Total phenolic content, flavonoid content, antioxidant capacity, reducing power and free-radical scavenging activity were determined colorimetrically. Read More

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September 2017

Sageretia thea (Osbeck.) mediated synthesis of zinc oxide nanoparticles and its biological applications.

Nanomedicine (Lond) 2017 Aug 12;12(15):1767-1789. Epub 2017 Jul 12.

UNESCO-UNISA Africa Chair in Nanoscience & Nanotechnology, College of Graduate Studies, University of South Africa, Pretoria, Gauteng, South Africa.

Aim: To investigate the physical and biological properties of bioinspired zinc oxide (ZnO) nanoparticles via aqueous leaf extracts of Sageretia thea.

Experimental: Nanoparticles of size approximately 12.4 nm were extensively characterized. Read More

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as a Potential Microalgal Growth-Promoting Bacterium in the Biosorption of Mercury from Aqueous Solutions.

J Microbiol Biotechnol 2017 Jun;27(6):1138-1149

Doctoral Program in Applied Sciences - Coastal Marine Systems, Faculty of Marine Sciences and Biological Resources, University of Antofagasta, Antofagasta 1270300, Chile.

The use of microalgal biomass is an interesting technology for the removal of heavy metals from aqueous solutions owing to its high metal-binding capacity, but the interactions with bacteria as a strategy for the removal of toxic metals have been poorly studied. The goal of the current research was to investigate the potential of co-immobilized with sp. in polyurethane discs for the biosorption of Hg(II) from aqueous solutions and to evaluate the influence of different Hg(II) concentrations (0. Read More

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Cutaneous leishmaniasis in Ouazzane and Sidi Kacem provinces, Morocco (1997-2012).

Bull Soc Pathol Exot 2016 Dec 19;109(5):376-380. Epub 2016 Sep 19.

Biology of Populations Laboratory, Faculty of sciences, Mohammed V Agdal University, Rabat, Morocco.

Cutaneous leishmaniasis (CL) is a major public health problem in Morocco. Three distinct parasites are involved; Leishmania tropica, Leishmania major and Leishmania infantum. The objective of this study is to investigate the epidemiological and the clinical features of endemic foci of CL in Sidi Kacem and Ouazzane provinces in the north of Morocco including molecular identification of parasites. Read More

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December 2016