54 results match your criteria trehalose participate


Integrative analysis of selected metabolites and the fungal transcriptome during the developmental cycle of strain G0119 correlates lignocellulose degradation with carbohydrate and triterpenoid metabolism.

Appl Environ Microbiol 2021 Apr 23. Epub 2021 Apr 23.

National Engineering Research Centre of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilisation, Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agriculture Sciences, Shanghai 201403, China

To systemically understand the biosynthetic pathways of bioactive substances, including triterpenoids and polysaccharides, in , the correlation between substrate degradation, carbohydrate and triterpenoid metabolism during growth was analyzed by combining changes in metabolite content and changes in related enzyme expression in over 5 growth phases. Changes in low-polarity triterpenoid content were correlated with changes in glucose and mannitol content in fruiting bodies. Additionally, changes in medium-polarity triterpenoid content were correlated with changes in the lignocellulose content of the substrate and with the glucose, trehalose and mannitol contents of fruiting bodies. Read More

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Transcriptional Dynamics of Genes Purportedly Involved in the Control of Meiosis, Carbohydrate, and Secondary Metabolism during Sporulation in .

Genes (Basel) 2021 Mar 29;12(4). Epub 2021 Mar 29.

Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.

spores (GLS), the mature germ cells ejected from the abaxial side of the pileus, have diverse pharmacological effects. However, the genetic regulation of sporulation in this fungus remains unknown. Here, samples corresponding to the abaxial side of the pileus were collected from strain YW-1 at three sequential developmental stages and were then subjected to a transcriptome assay. Read More

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Functional characterization of a novel, highly expressed ion-driven sugar antiporter in the thoracic muscles of Helicoverpa armigera.

Insect Sci 2021 Mar 22. Epub 2021 Mar 22.

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Sugar transporters (STs), which mainly mediate cellular sugar exchanges, play critical physiological roles in living organisms, and they may be responsible for sugar exchanges among various insect tissues. However, the molecular and physiological functions of insect STs are largely unknown. Here, 16 STs of Helicoverpa armigera were identified. Read More

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Neutron total scattering investigation on the dissolution mechanism of trehalose in NaOH/urea aqueous solution.

Struct Dyn 2021 Jan 10;8(1):014901. Epub 2021 Feb 10.

Institute for Advanced Study, Shenzhen University, Shenzhen 508060, China.

Trehalose is chosen as a model molecule to investigate the dissolution mechanism of cellulose in NaOH/urea aqueous solution. The combination of neutron total scattering and empirical potential structure refinement yields the most probable all-atom positions in the complex fluid and reveals the cooperative dynamic effects of NaOH, urea, and water molecules in the dissolution process. NaOH directly interacts with glucose rings by breaking the inter- and intra-molecular hydrogen bonding. Read More

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January 2021

Sequential changes in the regulatory mechanism of carbohydrate digestion in larvae of the silkworm, Bombyx mori.

J Comp Physiol B 2021 May 13;191(3):439-453. Epub 2021 Feb 13.

Laboratory of Developmental Biology, Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan.

Nutritional signals strictly control post-embryonic development in insects. Dietary carbohydrates are hydrolyzed to monosaccharides in the gut and then transported into the hemolymph. These monosaccharides in hemolymph are rapidly taken up by tissues and utilized in glycolysis, the pentose phosphate shunt, and glycogen or trehalose synthesis. Read More

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The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei.

J Dairy Sci 2021 Apr 5;104(4):4023-4032. Epub 2021 Feb 5.

Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin 150030, China; Kangyuan Dairy Co. Ltd., Yangzhou University, Yangzhou 225004, China. Electronic address:

This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS. We evaluated the EPS production capacity of 20 strains of L. paracasei under the same conditions. Read More

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Photovoltage generation by photosystem II core complexes immobilized onto a Millipore filter on an indium tin oxide electrode.

J Bioenerg Biomembr 2020 12 14;52(6):495-504. Epub 2020 Nov 14.

A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.

The light-induced functioning of photosynthetic pigment-protein complex of photosystem II (PSII) is linked to the vectorial translocation of charges across the membrane, which results in the formation of voltage. Direct measurement of the light-induced voltage (∆V) generated by spinach oxygen-evolving PSII core complexes adsorbed onto a Millipore membrane filter (MF) on an indium tin oxide (ITO) electrode under continuous illumination has been performed. PSII was shown to participate in electron transfer from water to the ITO electrode, resulting in ∆V generation. Read More

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December 2020

Energy disorders caused by mitochondrial dysfunction contribute to α-amatoxin-induced liver function damage and liver failure.

Toxicol Lett 2021 Jan 22;336:68-79. Epub 2020 Oct 22.

Chinese Center for Disease Control and Prevention, 29th Nanwei Road, Xicheng District, Beijing, 102206, China. Electronic address:

Mushroom toxicity is the main branch of foodborne poisoning, and liver damage caused by amatoxin poisoning accounts for more than 90 % of deaths due to mushroom poisoning. Alpha-amatoxin (α-AMA) has been considered the primary toxin from amatoxin-containing mushrooms, which is responsible for hepatotoxicity and death. However, the mechanism underlying liver failure due to α-AMA remains unclear. Read More

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January 2021

Targeting stress granules: A novel therapeutic strategy for human diseases.

Pharmacol Res 2020 11 16;161:105143. Epub 2020 Aug 16.

School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai, 201203, China. Electronic address:

Stress granules (SGs) are assemblies of mRNA and proteins that form from mRNAs stalled in translation initiation in response to stress. Chronic stress might even induce formation of cytotoxic pathological SGs. SGs participate in various biological functions including response to apoptosis, inflammation, immune modulation, and signalling pathways; moreover, SGs are involved in pathogenesis of neurodegenerative diseases, viral infection, aging, cancers and many other diseases. Read More

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November 2020

Coarse-Grained Molecular Model for the Glycosylphosphatidylinositol Anchor with and without Protein.

J Chem Theory Comput 2020 Jun 26;16(6):3889-3903. Epub 2020 May 26.

Max Planck Institute of Colloids and Interfaces, Potsdam 14476, Germany.

Glycosylphosphatidylinositol (GPI) anchors are a unique class of complex glycolipids that anchor a great variety of proteins to the extracellular leaflet of plasma membranes of eukaryotic cells. These anchors can exist either with or without an attached protein called GPI-anchored protein (GPI-AP) both and . Although GPIs are known to participate in a broad range of cellular functions, it is to a large extent unknown how these are related to GPI structure and composition. Read More

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Arabidopsis thaliana trehalose-6-phosphate phosphatase gene TPPI enhances drought tolerance by regulating stomatal apertures.

J Exp Bot 2020 07;71(14):4285-4297

Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

Transpiration occurs through stomata. The alteration of stomatal apertures in response to drought stress is an important process associated with water use efficiency (WUE). Trehalose-6-phosphate phosphatase (TPP) family genes have been reported to participate in adjustment of stomatal aperture. Read More

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The crystal structure of the mycobacterial trehalose monomycolate transport factor A, TtfA, reveals an atypical fold.

Proteins 2020 06 19;88(6):809-815. Epub 2019 Dec 19.

Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier, CNRS UMR 9004, Montpellier, France.

Trehalose monomycolate (TMM) represents an essential element of the mycobacterial envelope. While synthesized in the cytoplasm, TMM is transported across the inner membrane by MmpL3 but, little is known regarding the MmpL3 partners involved in this process. Recently, the TMM transport factor A (TtfA) was found to form a complex with MmpL3 and to participate in TMM transport, although its biological role remains to be established. Read More

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Genome-Wide Identification, Evolution, and Expression Analysis of and Gene Families in .

Plants (Basel) 2019 Sep 23;8(10). Epub 2019 Sep 23.

Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.

Trehalose biosynthesis enzyme homologues in plants contain two families, trehalose-6-phosphate synthases (TPSs) and trehalose-6-phosphate phosphatases (TPPs). Both families participate in trehalose synthesis and a variety of stress-resistance processes. Here, nine and ten genes were identified based on the genome, and all genes were classified into three classes. Read More

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September 2019

Putative trehalose biosynthesis proteins function as differential floridoside-6-phosphate synthases to participate in the abiotic stress response in the red alga Pyropia haitanensis.

BMC Plant Biol 2019 Jul 19;19(1):325. Epub 2019 Jul 19.

Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Post Box 71, Ningbo, 315211, Zhejiang Province, China.

Background: The heteroside floridoside is a primary photosynthetic product that is known to contribute to osmotic acclimation in almost all orders of Rhodophyta. However, the encoding genes and enzymes responsible for the synthesis of floridoside and its isomeric form, L- or D-isofloridoside, are poorly studied.

Results: Here, four putative trehalose-6-phosphate synthase (TPS) genes, designated as PhTPS1, PhTPS2, PhTPS3, and PhTPS4, were cloned and characterized from the red alga Pyropia haitanensis (Bangiophyceae). Read More

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In silico analysis of bacteriophage tail tubular proteins suggests a putative sugar binding site and a catalytic mechanism.

J Mol Graph Model 2019 11 7;92:8-16. Epub 2019 Jul 7.

L. Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Science, Department of Immunology and Infectious Diseases, ul. R. Weigla 12, 53-114, Wroclaw, PL, Poland.

Bacteriophage base tailplate proteins were recently discovered to have hydrolytic activity towards disaccharides. The putative assignment of sugar binding sites was based on known lectin structures and identified residues a.a. Read More

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November 2019

Trehalose protects Escherichia coli against carbon stress manifested by protein acetylation and aggregation.

Mol Microbiol 2019 09 19;112(3):866-880. Epub 2019 Jun 19.

Faculty of Biology, Department of General and Medical Biochemistry, University of Gdansk, Wita Stwosza 59, 80-308, Gdansk, Poland.

The disaccharide trehalose is widely distributed in nature and can serve as a carbon reservoir, a signaling molecule for controlling glucose metabolism and a stress protectant. We demonstrated that in Escherichia coli ΔotsA cells, which are unable to synthesize trehalose, the aggregation of endogenous proteins during the stationary phase was increased in comparison to wild-type cells. The lack of trehalose synthesis boosted Nε-lysine acetylation of proteins, which in turn enhanced their hydrophobicity and aggregation. Read More

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September 2019

Ectopic Expression of Causes Late-Flowering and Heterostylous Phenotypes in but not in .

Int J Mol Sci 2019 May 1;20(9). Epub 2019 May 1.

CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666303, China.

Trehalose-6-phosphate (T6P) phosphatase (TPP), a dephosphorylating enzyme, catalyzes the dephosphorylation of T6P, generating trehalose. In , we found six members of the family. Five of them , , , , and are highly expressed in female flowers or male flowers, or both, suggesting that members of the family may participate in flower development in . Read More

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Autophagy in Plants: Both a Puppet and a Puppet Master of Sugars.

Front Plant Sci 2019 22;10:14. Epub 2019 Jan 22.

Laboratory of Molecular Plant Biology, KU Leuven, Leuven, Belgium.

Autophagy is a major pathway that recycles cellular components in eukaryotic cells both under stressed and non-stressed conditions. Sugars participate both metabolically and as signaling molecules in development and response to various environmental and nutritional conditions. It is therefore essential to maintain metabolic homeostasis of sugars during non-stressed conditions in cells, not only to provide energy, but also to ensure effective signaling when exposed to stress. Read More

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January 2019

Allosteric activation of yeast enzyme neutral trehalase by calcium and 14-3-3 protein.

Physiol Res 2019 04 10;68(2):147-160. Epub 2019 Jan 10.

Department of Structural Biology of Signaling Proteins, Division BIOCEV, Institute of Physiology of the Czech Academy of Sciences, Vestec, Czech Republic.

Neutral trehalase 1 (Nth1) from Saccharomyces cerevisiae catalyzes disaccharide trehalose hydrolysis and helps yeast to survive adverse conditions, such as heat shock, starvation or oxidative stress. 14-3-3 proteins, master regulators of hundreds of partner proteins, participate in many key cellular processes. Nth1 is activated by phosphorylation followed by 14-3-3 protein (Bmh) binding. Read More

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Growth in Hyper-Concentrated Sweet Whey Triggers Multi Stress Tolerance and Spray Drying Survival in BL23: From the Molecular Basis to New Perspectives for Sustainable Probiotic Production.

Front Microbiol 2018 22;9:2548. Epub 2018 Oct 22.

UMR1253 STLO, Agrocampus Ouest, INRA, Rennes, France.

BL23 has a recognized probiotic potential, which includes immune modulation, protection toward induced colitis, toward induced colon cancer and toward dissemination of pathogens. In , as well as in other probiotics, both probiotic and technological abilities are highly dependent (1) on the substrate used to grow bacteria and (2) on the process used to dry and store this biomass. Production and storage of probiotics, at a reasonable financial and environmental cost, becomes a crucial challenge. Read More

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October 2018

Circulating glucose levels inversely correlate with larval feeding through insulin signaling and SLC5A11.

Commun Biol 2018 13;1:110. Epub 2018 Aug 13.

Department of Developmental Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390, USA.

In mammals, blood glucose levels likely play a role in appetite regulation yet the mechanisms underlying this phenomenon remain opaque. Mechanisms can often be explored from genetic approaches. To determine if circulating sugars might be involved in feeding behaviors, we scored hemolymph glucose and trehalose, and food ingestion in larvae subjected to various diets, genetic mutations, or RNAi. Read More

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Time-Course Transcriptomics Analysis Reveals Key Responses of Submerged Deepwater Rice to Flooding.

Plant Physiol 2018 04 23;176(4):3081-3102. Epub 2018 Feb 23.

Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi 464-8601, Japan

Water submergence is an environmental factor that limits plant growth and survival. Deepwater rice () adapts to submergence by rapidly elongating its internodes and thereby maintaining its leaves above the water surface. We performed a comparative RNA sequencing transcriptome analysis of the shoot base region, including basal nodes, internodes, and shoot apices of seedlings at two developmental stages from two varieties with contrasting deepwater growth responses. Read More

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Association of C-Type Lectin Mincle with FcεRIβγ Subunits Leads to Functional Activation of RBL-2H3 Cells through Syk.

Sci Rep 2017 04 10;7:46064. Epub 2017 Apr 10.

Division of Genome Science and Microbiology, Department of Pathological Sciences, University of Fukui, 23-3 Matsuoka-Shimoaizuki, Eiheiji, Fukui 910-1193, Japan.

Macrophage-inducible C-type lectin (Mincle) interacts with the γ-subunit of high-affinity IgE receptor (FcεRIγ) and activates Syk by recognizing its specific ligand, trehalose-6,6'-dimycolate, a glycolipid produced by Mycobacterium tuberculosis. It has been suggested that mast cells participate in the immune defense against pathogenic microbes including M. tuberculosis, although the functions are still uncertain. Read More

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Comparative metagenomics reveals insights into the deep-sea adaptation mechanism of the microorganisms in Iheya hydrothermal fields.

World J Microbiol Biotechnol 2017 May 6;33(5):86. Epub 2017 Apr 6.

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China.

In this study, comparative metagenomic analysis was performed to investigate the genetic profiles of the microbial communities inhabiting the sediments surrounding Iheya North and Iheya Ridge hydrothermal fields. Four samples were used, which differed in their distances from hydrothermal vents. The results showed that genes involved in cell surface structure synthesis, polyamine metabolism and homeostasis, osmoadaptation, pH and Na homeostasis, and heavy-metal transport were abundant. Read More

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Structure of Aqueous Trehalose Solution by Neutron Diffraction and Structural Modeling.

J Phys Chem B 2016 12 5;120(49):12669-12678. Epub 2016 Dec 5.

ISIS Pulsed Neutron and Muon Source, STFC Rutherford Appleton Laboratory, Harwell Campus , Oxon, OX11 0QX, Oxfordshire, U.K.

The molecular structure of an aqueous solution of the disaccharide trehalose (CHO) has been studied by neutron diffraction and empirical potential structure refinement modeling. Six different isotope compositions with 33 wt % trehalose (corresponding to 38 water molecules per trehalose molecule) were measured to ensure that water-water, trehalose-water, and trehalose-trehalose correlations were accurately determined. In fact, this is the first neutron diffraction study of an aqueous trehalose solution in which also the nonexchangeable hydrogen atoms in trehalose are deuterated. Read More

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December 2016

Genome-Wide Identification and Evolution Analysis of Trehalose-6-Phosphate Synthase Gene Family in .

Front Plant Sci 2016 29;7:1445. Epub 2016 Sep 29.

College of Horticulture, Nanjing Agricultural University Nanjing, China.

Trehalose-6-phosphate synthase (TPS) plays a key role in plant carbohydrate metabolism and the perception of carbohydrate availability. In the present work, the publicly available (lotus) genome sequence database was analyzed which led to identification of nine lotus genes (). It was found that at least two introns are included in the coding sequences of genes. Read More

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September 2016

Transcriptome Analysis of Sucrose Metabolism during Bulb Swelling and Development in Onion ( L.).

Front Plant Sci 2016 22;7:1425. Epub 2016 Sep 22.

Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China) Beijing, China.

L. is a widely cultivated and economically significant vegetable crop worldwide, with beneficial dietary and health-related properties, but its sucrose metabolism is still poorly understood. To analyze sucrose metabolism during bulb swelling, and the development of sweet taste in onion, a global transcriptome profile of onion bulbs was undertaken at three different developmental stages, using RNA-seq. Read More

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September 2016

Metabolite transport and associated sugar signalling systems underpinning source/sink interactions.

Biochim Biophys Acta 2016 10 31;1857(10):1715-25. Epub 2016 Jul 31.

Centre for Plant Sciences, School of Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK. Electronic address:

Metabolite transport between organelles, cells and source and sink tissues not only enables pathway co-ordination but it also facilitates whole plant communication, particularly in the transmission of information concerning resource availability. Carbon assimilation is co-ordinated with nitrogen assimilation to ensure that the building blocks of biomass production, amino acids and carbon skeletons, are available at the required amounts and stoichiometry, with associated transport processes making certain that these essential resources are transported from their sites of synthesis to those of utilisation. Of the many possible posttranslational mechanisms that might participate in efficient co-ordination of metabolism and transport only reversible thiol-disulphide exchange mechanisms have been described in detail. Read More

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October 2016

Mycobacterial glycolipids di-O-acylated trehalose and tri-O-acylated trehalose downregulate inducible nitric oxide synthase and nitric oxide production in macrophages.

BMC Immunol 2015 Jun 23;16:38. Epub 2015 Jun 23.

Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico.

Background: Tuberculosis (TB) remains a serious human health problem that affects millions of people in the world. Understanding the biology of Mycobacterium tuberculosis (Mtb) is essential for tackling this devastating disease. Mtb possesses a very complex cell envelope containing a variety of lipid components that participate in the establishment of the infection. Read More

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Genome sequence of the Drosophila melanogaster male-killing Spiroplasma strain MSRO endosymbiont.

mBio 2015 Mar 31;6(2). Epub 2015 Mar 31.

INRA, University of Bordeaux, Unité Mixte de Recherche 1332, Villenave d'Ornon, France

Unlabelled: Spiroplasmas are helical and motile members of a cell wall-less eubacterial group called Mollicutes. Although all spiroplasmas are associated with arthropods, they exhibit great diversity with respect to both their modes of transmission and their effects on their hosts; ranging from horizontally transmitted pathogens and commensals to endosymbionts that are transmitted transovarially (i.e. Read More

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