Trop Med Int Health 2021 Sep 12. Epub 2021 Sep 12.
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Objectives: The aims of the study are twofold: (1) antigen (Ag) preparation and evaluation of three antigens of Gnathostoma spinigerum infective larvae (GsL3), crude somatic antigen (CSAg), excretory-secretory antigen (ESAg), and partially purified antigens (namely, P1Ag, P2Ag, and P3Ag) to differentiate IgE, IgG, IgG1-4, and IgM for human gnathostomiasis diagnosis; (2) application of the selected ELISA for following up stored sera of patients treated with ivermectin (IVM), and albendazole (ABZ).
Methods: Different antigens were analyzed by antibodies of gnathostomiasis cases, other parasite infections, and healthy controls using indirect ELISA to differentiate IgE, IgG, IgG1-4, and IgM. Then, prominent antigen and immunoglobulin were used in antibody predictions of gnathostomiasis cases treated with albendazole or ivermectin. Read More