7,227 results match your criteria treated larvae

Silencing uridine diphosphate N-acetylglucosamine pyrophosphorylase gene impairs larval development in Henosepilachna vigintioctopunctata.

Pest Manag Sci 2021 Sep 14. Epub 2021 Sep 14.

Agriculture Ministry Key Laboratory of Integrated Pest Management on Crops in East China/State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.

Background: Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) diphosphorylase (UAP) catalyzes the formation of UDP-GlcNAc, the precursor for production of chitin in the ectodermally-derived epidermal cells and midgut, for GlcNAcylation of proteins and for generation of glycosyl-phosphatidyl-inositol anchors in all tissues in Drosophila melanogaster.

Results: Here we identified a putative HvUAP gene in Henosepilachna vigintioctopunctata. Knockdown of HvUAP at the second, third and fourth instar stages impaired larval development. Read More

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September 2021

Dietary cadmium (Cd) reduces hemocyte level by induction of apoptosis in Drosophila melanogasterg.

Comp Biochem Physiol C Toxicol Pharmacol 2021 Sep 10:109188. Epub 2021 Sep 10.

Department of Life Sciences, Central University of Jharkhand, Ratu-Lohardaga Road, Brambe, Ranchi 835205, Jharkhand, India. Electronic address:

Drosophila melanogaster larvae ensure continuous proliferation and differentiation of hemocytes to maintain a fixed range of different blood cell types during its various stages of development. Variation in this number is often an indicator of animal well-being, its genotype or an effect of environmental perturbation, including exposure to heavy metals. The present study investigates the effect of Cd on larval hemocytes. Read More

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September 2021

Evaluation of (Graviola) leaves activity against experimental trichinellosis: and studies.

J Helminthol 2021 Sep 13;95:e53. Epub 2021 Sep 13.

Department of Medical Parasitology, kafrelsheikh Faculty of Medicine, Kafrelsheikh University, ElGiesh street, kafrelsheikh governorate, 33516, Egypt.

Our work aimed to evaluate the possible effect of Annona muricata (Graviola) leaf extract on Trichinella spiralis in in vitro and in vivo studies. Trichinella spiralis worms were isolated from infected mice and transferred to three culture media - group I (with no drugs), group II (contained Graviola) and group III (contained albendazole) - then they were examined using the electron microscope. In the in vivo study, mice were divided into five groups: GI (infected untreated), GII (prophylactically treated with Graviola for seven days before infection), GIII (infected and treated with Graviola), GIV (infected and treated with albendazole) and GV (infected and treated with a combination of Graviola plus albendazole in half doses). Read More

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September 2021

Prevention of Saprolegniasis in rainbow trout (Oncorhynchus mykiss) eggs using oregano (Origanum onites) and laurel (Laurus nobilis) essential oils.

J Fish Dis 2021 Sep 13. Epub 2021 Sep 13.

Department of Forest Botany, Kastamonu University Forestry Faculty, Kastamonu, Turkey.

The present study investigated the antifungal effects of essential oils of oregano (Origanum onites) and laurel (Laurus nobilis) on Saprolegniasis, a disease that occurs in rainbow trout eggs during the incubation period. Oregano and laurel were ground after drying, and essential oils were obtained by water distillation method using a Clevenger apparatus. The essential oils were added to potato dextrose agar (PDA) at the rates of 1-1000 ppm, and the minimum inhibitory concentration (MIC) was determined as 250 ppm whereas the minimum lethal concentration (MLC) was determined to be 500 ppm for both plants. Read More

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September 2021

Evaluation of immunodiagnostic tests for human gnathostomiasis using different antigen preparations of Gnathostoma spinigerum larvae against IgE, IgM, IgG, IgG1-4, and IgG1 patterns of post-treated patients.

Trop Med Int Health 2021 Sep 12. Epub 2021 Sep 12.

Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

Objectives: The aims of the study are twofold: (1) antigen (Ag) preparation and evaluation of three antigens of Gnathostoma spinigerum infective larvae (GsL3), crude somatic antigen (CSAg), excretory-secretory antigen (ESAg), and partially purified antigens (namely, P1Ag, P2Ag, and P3Ag) to differentiate IgE, IgG, IgG1-4, and IgM for human gnathostomiasis diagnosis; (2) application of the selected ELISA for following up stored sera of patients treated with ivermectin (IVM), and albendazole (ABZ).

Methods: Different antigens were analyzed by antibodies of gnathostomiasis cases, other parasite infections, and healthy controls using indirect ELISA to differentiate IgE, IgG, IgG1-4, and IgM. Then, prominent antigen and immunoglobulin were used in antibody predictions of gnathostomiasis cases treated with albendazole or ivermectin. Read More

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September 2021

A case of breast sparganosis: with an emphasis on ultrasonographic findings.

Radiol Case Rep 2021 Nov 1;16(11):3389-3391. Epub 2021 Sep 1.

Department of Nuclear Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, No. 169 Donghu Road, Wuhan, Hubei Province, 430071, China.

Sparganosis is a rare disease caused by the infestation of the plerocercoid tapeworm larva of the genus Spirometra. Human sparganosis is most commonly encountered in subcutaneous fat areas of the abdomen, limbs, and genitourinary tract. Breast sparganosis occur very rarely, accounting for less than 2% of total human sparganosis cases. Read More

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November 2021

Involvement of Chemosensory Protein BodoCSP1 in Perception of Host Plant Volatiles in .

J Agric Food Chem 2021 Sep 10. Epub 2021 Sep 10.

State Key Laboratory of Crop Stress Biology for Arid Areas, and Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.

Chemosensory proteins (CSPs) can bind and transport odorant molecules and play important roles in insect chemoreception. In this study, we focused on the roles of a chemosensory protein (BodoCSP1) in perception of host plant volatiles in . The expression of was significantly higher in adults than in larvae and pupae, without a significant difference between male and female adults. Read More

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September 2021

Honey bee Apis mellifera larvae gut microbial and immune, detoxication responses towards flumethrin stress.

Environ Pollut 2021 Sep 4;290:118107. Epub 2021 Sep 4.

Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, 330045, PR China; Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang, 330045, PR China. Electronic address:

Mites are considered the worst enemy of honey bees, resulting in economic losses in agricultural production. In apiculture, flumethrin is frequently used to control mites. It causes residues of flumethrin in colonies which may threaten honey bees, especially for larvae. Read More

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September 2021

Efficacy of low-dose fipronil bait against blacklegged tick (Ixodes scapularis) larvae feeding on white-footed mice (Peromyscus leucopus) under simulated field conditions.

Parasit Vectors 2021 Sep 7;14(1):459. Epub 2021 Sep 7.

Genesis Laboratories, Inc., Wellington, CO, USA.

Background: Lyme disease, caused primarily by Borrelia burgdorferi sensu stricto, is the most prevalent vector-borne disease in the United States. Treatment of rodent pathogen reservoirs with an oral acaricide may suppress the production of infected host-seeking ticks posing a risk for human infection. A previous study showed that an oral fipronil bait effectively controlled larval Ixodes scapularis ticks on white-footed mice (Peromyscus leucopus) up to 15 days post-bait exposure. Read More

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September 2021

Targeted Propolis-Loaded Poly (Butyl) Cyanoacrylate Nanoparticles: An Alternative Drug Delivery Tool for the Treatment of Cryptococcal Meningitis.

Front Pharmacol 2021 20;12:723727. Epub 2021 Aug 20.

Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.

In this study, we describe a nano-carrier system for propolis that is able to cross an model of the blood-brain barrier (BBB) and effectively reduce the virulence of in animal models. Antimicrobial properties of propolis have been widely studied. However, propolis applications are limited by its low water solubility and poor bioavailability. Read More

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Chitin metabolism as a potential target for RNAi-based control of the forestry pest Hyblaea puera Cramer (Lepidoptera: Hyblaeidae).

Pest Manag Sci 2021 Sep 6. Epub 2021 Sep 6.

Plant Biotechnology and Cytogenetics Division, Institute of Forest Genetics and Tree Breeding, R. S. Puram, Coimbatore, 641002.

Background: H. puera, commonly known as the teak defoliator, is a serious pest in teak plantations. Despite the availability of control measures, this pest causes losses in yield and quality of timber, through voracious feeding. Read More

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September 2021

Monthly biological larviciding associated with a tenfold decrease in larval density in fish farming ponds and reduced community-wide malaria incidence in northwestern Brazil.

Parasit Vectors 2021 Sep 3;14(1):445. Epub 2021 Sep 3.

Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 1374, São Paulo, SP, 05508-900, Brazil.

Background: Larvicides are typically applied to fixed and findable mosquito breeding sites, such as fish farming ponds used in commercial aquaculture, to kill immature forms and thereby reduce the size of adult malaria vector populations. However, there is little evidence suggesting that larviciding may suppress community-wide malaria transmission outside Africa. Here, we tested whether the biological larvicide VectoMax FG applied at monthly intervals to fish farming ponds can reduce malaria incidence in Amazonian Brazil. Read More

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September 2021

Effects of Prohydrojasmon on the Number of Infesting Herbivores and Biomass of Field-Grown Japanese Radish Plants.

Front Plant Sci 2021 12;12:695701. Epub 2021 Aug 12.

Center for Ecological Research, Kyoto University, Otsu, Japan.

Prohydrojasmon (PDJ), an analog of jasmonic acid (JA), was found to induce direct and indirect defenses against herbivores in non-infested plants. To test whether PDJ can be used for pest control in crop production, we conducted experiments in pesticide-free Japanese radish fields from October 4 to December 12 in 2015. Twenty-four Japanese radish plants in three plots were treated with a 100 times-diluted commercial formulation (5%) of PDJ (treated plants), and 24 plants in three different plots were treated with water (control plants) until November 29 every week. Read More

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Water Extracts Attenuate Atherosclerosis.

Mediators Inflamm 2021 23;2021:9951946. Epub 2021 Aug 23.

School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198 Jiangsu, China.

Objectives: . (DH) is a Chinese herbal medicine, which is often used to make tea to improve immunity in China. Rumor has it that DH has a protective effect against cardiovascular disease. Read More

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First report on reduced efficacy of ivermectin on Oesophagostomum spp. on Swedish pig farms.

Vet Parasitol Reg Stud Reports 2021 Jul 10;25:100598. Epub 2021 Jun 10.

Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health, Section for Parasitology, Box 7036, 750 07 Uppsala, Sweden.

Anthelmintic efficacy was investigated in nine sow herds that had been identified with high faecal egg counts in a prevalence study. Faecal samples were collected from a total of 104 individual sows, and analysed using a centrifugal flotation McMaster technique. Samples positive for strongyle eggs were cultured to third stage larvae (L3) for genus identification and then further identified to Oesophagostomum species by sequencing. Read More

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Sex-specific effects of the microbiota on adult carbohydrate intake and body composition in a polyphagous fly.

J Insect Physiol 2021 Aug 30;134:104308. Epub 2021 Aug 30.

Department of Biological Sciences, Macquarie University, Sydney, NSW, Australia.

The microbiota influences hosts' health and fitness. However, the extent to which the microbiota affects host' foraging decisions and related life history traits remains to be fully understood. Our study explored the effects of microbiota manipulation on foraging preference and phenotypic traits of larval and adult stages of the polyphagous fruit fly Bactrocera tryoni, one of the main horticultural pests in Australia. Read More

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Effects of itol A on the larval growth and development of Spodoptera frugiperda (Lepidoptera: Noctuidae).

Pest Manag Sci 2021 Aug 28. Epub 2021 Aug 28.

Guangxi Key Laboratory of Agric-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, 530004, People's Republic of China.

Background: Itol A, extracted from the Itoa orientalis Hemsl. (Flacourtiaceae), possesses bioactivity on Spodoptera litura (Lepidoptera: Noctuidae) and Nilaparvata lugens (Stål) (Hemiptera: Delphacidae). Our previous study showed that the effects on Spodoptera frugiperda, a destructive pest found worldwide, were similar to those of fenoxycarb (FC), a juvenile hormone analog (JHA). Read More

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RNAi for chitin synthase 1 rather than 2 causes growth delay and molting defect in Henosepilachna vigintioctopunctata.

Pestic Biochem Physiol 2021 Oct 17;178:104934. Epub 2021 Jul 17.

Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

Chitin synthase (CHS) plays a critical role in chitin synthesis and excretion. In most insects, CHSs have been segregated into 1 and 2 classes. CHS1 is responsible for chitin production in the ectodermally-derived epidermal cells. Read More

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October 2021

Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway of .

Int J Mol Sci 2021 Aug 23;22(16). Epub 2021 Aug 23.

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, China.

is a worldwide pest afflicting edible vegetables and has developed varying levels of resistance to insecticides. Methoxyfenozide (MET), an ecdysteroid agonist, is effective against lepidopteran pests such as . However, the mechanism of MET to remains unclear. Read More

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Efficient Neuroprotective Rescue of Sacsin-Related Disease Phenotypes in Zebrafish.

Int J Mol Sci 2021 Aug 5;22(16). Epub 2021 Aug 5.

Neurobiology and Molecular Medicine, IRCCS Fondazione Stella Maris, 56128 Pisa, Italy.

Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is a multisystem hereditary ataxia associated with mutations in , which encodes sacsin, a protein of still only partially understood function. Although mouse models of ARSACS mimic largely the disease progression seen in humans, their use in the validation of effective therapies has not yet been proposed. Recently, the teleost has attracted increasing attention as a vertebrate model that allows rapid and economical screening, of candidate molecules, and thus combines the advantages of whole-organism phenotypic assays and in vitro high-throughput screening assays. Read More

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Toxicity, Sublethal and Low Dose Effects of Imidacloprid and Deltamethrin on the Aphidophagous Predator (Coleoptera: Coccinellidae).

Insects 2021 Aug 3;12(8). Epub 2021 Aug 3.

Laboratory of Agricultural Entomology and Zoology, Department of Agriculture, Kalamata Campus, University of the Peloponnese, 24100 Antikalamos, Greece.

(Coleoptera: Coccinellidae) is a common aphidophagous coccinellid predator used in biological control against aphid pests. Knowing toxicity, lethal, and sublethal effects of insecticides on natural enemies is essential in order to incorporate them into Integrated Pest Management (IPM). In the present study, the lethal and sublethal effects of imidacloprid and deltamethrin were evaluated on the fourth instar larvae of and subsequently on the full life cycle. Read More

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Lipopolysaccharides Enhance Epithelial Hyperplasia and Tubular Adenoma in Intestine-Specific Expression of in Transgenic Zebrafish.

Biomedicines 2021 Aug 7;9(8). Epub 2021 Aug 7.

Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.

Intestinal carcinogenesis is a multistep process that begins with epithelial hyperplasia, followed by a transition to an adenoma and then to a carcinoma. Many etiological factors, including mutations and inflammation, have been implicated in oncogenesis. However, the potential synergistic effects between mutations and inflammation as well as the potential mechanisms by which they promote intestinal carcinogenesis remain unclear. Read More

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The Efficacy of Enriched with Probiotic in Enhancing Survival and Disease Resistance of Red Hybrid Tilapia ( spp.) Larvae.

Antibiotics (Basel) 2021 Aug 16;10(8). Epub 2021 Aug 16.

Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Malaysia.

The administration of probiotics via live feeds, such as and rotifers, has gained significant attention. Moreover, indiscriminate use of antibiotics in conventional aquaculture practices in order to prevent or control disease outbreaks has resulted in the occurrence of residues and antimicrobial resistance. Thus, the application of eco-friendly feed additives, such as probiotics, as a safer alternative has received increasing attention in recent years. Read More

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Green Synthesis of Selenium Nanoparticles Mediated by Nilgirianthus ciliates Leaf Extracts for Antimicrobial Activity on Foodborne Pathogenic Microbes and Pesticidal Activity Against Aedes aegypti with Molecular Docking.

Biol Trace Elem Res 2021 Aug 24. Epub 2021 Aug 24.

Department of Zoology, School of Life Sciences, Periyar University, Salem, 636011, India.

The present study deals with the synthesis of selenium nanoparticles (SeNPs) using Nilgirianthus ciliatus leaf extracts, characterized by UV-Vis spectrophotometer, XRD, FTIR, FE-SEM, HR-TEM, DLS, and zeta potential analysis. The antimicrobial activity against Staphylococcus aureus (MTCC96), Escherichia coli (MTCC443), and Salmonella typhi (MTCC98) showed the remarkable inhibitory effect at 25 µl/mL concentration level. Furthermore, the characterized SeNPs showed a great insecticidal activity against Aedes aegypti in the early larval stages with the median Lethal Concentration (LC) of 0. Read More

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Application of the Paracentrotus lividus sea-urchin embryo-larval bioassay to the marine pollution biomonitoring program in the Tunisian coast.

Environ Sci Pollut Res Int 2021 Aug 24. Epub 2021 Aug 24.

Research Laboratory of Bioresources: Integrative Biology & Valorization (LR 14ES06), Higher Institute of Biotechnology of Monastir, Avenue Tahar HAdded, 74,5000, Monastir, PB, Tunisia.

The pollution of the marine environment by treated and untreated effluents has increased due to human activities. Monitoring the marine ecosystem is nowadays a global concern. In this work, we evaluated the effect of contaminated and uncontaminated seawater, from different Tunisian coastal areas, on the fertilization, gastrulation, and embryo-larval development events of sea urchins (Paracentrotus lividus). Read More

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A zebrafish model for calcineurin-dependent brain function.

Behav Brain Res 2021 Aug 20;416:113544. Epub 2021 Aug 20.

Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA. Electronic address:

Small-molecule modulators of calcineurin signaling have been proposed as potential therapeutics in Down syndrome and Alzheimer's disease. Models predict that in Down syndrome, suppressed calcineurin-NFAT signaling may be mitigated by proINDY, which activates NFAT, the nuclear factor of activated T-cells. Conversely, elevated calcineurin signaling in Alzheimer's disease may be suppressed with the calcineurin inhibitors cyclosporine and tacrolimus. Read More

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Effect of environmental variables and their interaction on gordiid hairworm larvae (Nematomorpha).

J Helminthol 2021 Aug 23;95:e47. Epub 2021 Aug 23.

Programa de Ecofisiología Aplicada (PRODEA), Instituto de Ecología y Desarrollo Sustentable (INEDES) UNLu-CONICET y Departamento de Ciencias Básicas. Universidad Nacional de Luján, Luján, 6700Buenos Aires, Argentina.

The different stages of the life cycle of parasites are important components of ecosystems. Changes in environmental conditions may affect free-living stages, host-parasite interactions and ecosystem functioning. The larvae of Chordodes nobilii, which belongs to the parasitic phylum Nematomorpha, are susceptible to extreme temperatures and different pollutants, but the effects of pH and moderate temperature variations have not been evaluated yet. Read More

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Sodium valproate increases activity of the sirtuin pathway resulting in beneficial effects for spinocerebellar ataxia-3 in vivo.

Mol Brain 2021 08 20;14(1):128. Epub 2021 Aug 20.

Centre for Motor Neuron Disease Research, Department of Biomedical Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, Level 1, 2 Technology Place, Sydney, NSW 2109, Australia.

Machado-Joseph disease (MJD, also known as spinocerebellar ataxia type 3) is a fatal neurodegenerative disease that impairs control and coordination of movement. Here we tested whether treatment with the histone deacetylase inhibitor sodium valproate (valproate) prevented a movement phenotype that develops in larvae of a transgenic zebrafish model of the disease. We found that treatment with valproate improved the swimming of the MJD zebrafish, affected levels of acetylated histones 3 and 4, but also increased expression of polyglutamine expanded human ataxin-3. Read More

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Description of malaria epidemics and normal transmissions using rainfall variability in Gondar Zuria highland District, Ethiopia.

Heliyon 2021 Aug 27;7(8):e07653. Epub 2021 Jul 27.

College of Medicine and Health Sciences, School of Biomedical and Laboratory Sciences, Department of Medical Parasitology, University of Gondar, Ethiopia.

Background: Rainfall is one of the climate variables most studied as it affects malaria occurrence directly.

Objective: This study aimed to describe how monthly rainfall variability affects malaria incidence in different years.

Methods: A total of 7 years (2013/14-2019/20) retrospective confirmed and treated malaria cases in Gondar Zuria district were used for analysis in addition to five (2013/14-2017/18) years retrospective data from Dembia district. Read More

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The Anti-Biofilm Efficacy of Caffeic Acid Phenethyl Ester (CAPE) and a Murine Model of Oral Candidiasis.

Front Cell Infect Microbiol 2021 2;11:700305. Epub 2021 Aug 2.

Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos, Brazil.

is the main fungal species associated with the development of oral candidiasis. Currently, therapeutic options for these infections are limited by the adverse effects of antifungal drugs and by the emergence of drug resistant strains. Thus, the development of new antifungal agents is needed for the prevention and treatment of oral infections. Read More

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