358,429 results match your criteria transferase activity

Impacts of wildfires in aquatic organisms: biomarker responses and erythrocyte nuclear abnormalities in Gambusia holbrooki exposed in situ.

Environ Sci Pollut Res Int 2021 May 14. Epub 2021 May 14.

CESAM - Centre for Environmental and Marine Studies, University of Aveiro, Aveiro, Portugal.

Wildfires are an environmental concern due to the loss of forest area and biodiversity, but also because their role as drivers of freshwater systems contamination by metals. In this context, the fish Gambusia holbrooki was used as a model, deployed for in situ exposure in watercourses standing within a recently burnt area and further assessment of toxic effects. The fish were exposed during 4 days at four different sites: one upstream and another downstream the burnt area and two within the burnt area. Read More

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Effects of exposure to the UV-filter 4-MBC during Solea senegalensis metamorphosis.

Environ Sci Pollut Res Int 2021 May 13. Epub 2021 May 13.

CESAM-Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal.

Many personal care products integrate UV-filters, such as 4-methylbenzylidene camphor (4-MBC), a compound frequently detected in aquatic habitats, including coastal areas. However, the potential effects of 4-MBC to saltwater species have been poorly studied. Therefore, the main objective of this work is to study the effects of 4-MBC exposure on Solea senegalensis during metamorphosis, a sensitive life stage of this flatfish. Read More

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Activity of Vip3Aa1 against .

Open Life Sci 2020 24;15:133-144. Epub 2020 Mar 24.

School of Pharmaceutical Sciences, Southern Medical University,1023 Shatai South Road, Guangzhou 510515, P. R. China.

() is a well-known entomopathogen. In this study, we cloned the gene from strain GIM1.147 and investigated the insecticidal activity of Vip3Aa1 protein produced by against and . Read More

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Transcriptome sequencing and analysis reveals the molecular mechanism of skeletal muscle atrophy induced by denervation.

Ann Transl Med 2021 Apr;9(8):697

Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, China.

Background: The molecular mechanism of denervated muscle atrophy is very complex and has not been elucidated to date. In this study, we aimed to use transcriptome sequencing technology to systematically analyze the molecular mechanism of denervated muscle atrophy in order to eventually develop effective strategies or drugs to prevent muscle atrophy.

Methods: Transcriptome sequencing technology was used to analyze the differentially expressed genes (DEGs) in denervated skeletal muscles. Read More

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Boldenone Undecylenate-Mediated Hepatorenal Impairment by Oxidative Damage and Dysregulation of Heat Shock Protein 90 and Androgen Receptors Expressions: Vitamin C Preventive Role.

Front Pharmacol 2021 27;12:651497. Epub 2021 Apr 27.

Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.

Boldenone Undecylenate (BLD) is a synthetic derivative of testosterone and a widely used anabolic androgenic steroid. The health risk of BLD use as a pharmaceutical or dietary supplement is still underestimated and under-reported. Vitamin C (VC) has been recognized as an antioxidant with prominent hepatorenal protective effects. Read More

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C646 inhibits G2/M cell cycle-related proteins and potentiates anti-tumor effects in pancreatic cancer.

Sci Rep 2021 May 12;11(1):10078. Epub 2021 May 12.

Department of Hepatobiliary and Pancreatic Surgery, Graduate School of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan.

The activity of histone acetyltransferases (HATs) plays a central role in an epigenetic modification in cooperation with HDACs (histone deacetyl transferases). It is likely that malfunction of this enzymatic machinery controlling epigenetic modification is relevant to carcinogenesis and tumor progression. However, in pancreatic cancer, the clinical relevance of HAT activity and histone acetylation has remained unclear. Read More

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Assessment of carbamazepine acute toxicity in the cockle Cerastoderma edule through chemical, physiological and biochemical tools.

Braz J Biol 2021 10;82:e247035. Epub 2021 May 10.

University of Carthage, Laboratory of Biomonitoring of the Environment LR01 ES14, Faculty of Sciences of Bizerte, Zarzouna 7021, Tunisia.

The cockle Cerastoderma edule was exposed to four concentrations (5, 10, 20 and 70 μg L-1) of carbamazepine (CBZ). This anticonvulsant was found to alter the mussel behavior of by reducing its clearance rate (CR). Analysis of CBZ accumulation in tissues of C. Read More

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[Identification and pedigree analysis for an A(W)37B subtype due to c.940A>G variant of ABO gene].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 May;38(5):496-498

School of Medical Laboratory, Weifang Medical University, Weifang, Shandong 261053, China.

Objective: To delineate the serological and molecular profiles of a patient with A(w)37B subtype.

Methods: The ABO bloodtypes of the proband, his wife and daughter were determined with a standard serological method. Their ABO genotypes were determined by sequence-specific primer polymerase chain reaction (PCR-SSP). Read More

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Nanosilver and Silver Nitrate Toxicity in Ex Vivo-Exposed Gills of Fish and Mitigation by Humic Acids.

Bull Environ Contam Toxicol 2021 May 11. Epub 2021 May 11.

Instituto Nacional de Limnología, UNL, CONICET, Santa Fe, Argentina.

Silver nanoparticles (AgNP) are unique because of their biocide properties. Once released to environment, AgNP interact with the natural organic matter which impact on their fate, dispersion, and ultimate toxicity. We carried out an ex vivo exposure of gill of Corydoras paleatus fish to 100 µg L of AgNP or AgNO, alone and in combination with 10 mg L of humic acids (HA), with the aim to evaluate the potential mitigation of HA on AgNP toxic effects. Read More

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African Americans and European Americans exhibit distinct gene expression patterns across tissues and tumors associated with immunologic functions and environmental exposures.

Sci Rep 2021 05 10;11(1):9905. Epub 2021 May 10.

Bioinformatics and Computational Biology Program, Iowa State University, Ames, IA, 50011, USA.

The COVID-19 pandemic has affected African American populations disproportionately with respect to prevalence, and mortality. Expression profiles represent snapshots of combined genetic, socio-environmental (including socioeconomic and environmental factors), and physiological effects on the molecular phenotype. As such, they have potential to improve biological understanding of differences among populations, and provide therapeutic biomarkers and environmental mitigation strategies. Read More

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Genetic analysis in European ancestry individuals identifies 517 loci associated with liver enzymes.

Nat Commun 2021 May 10;12(1):2579. Epub 2021 May 10.

Department of Epidemiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.

Serum concentration of hepatic enzymes are linked to liver dysfunction, metabolic and cardiovascular diseases. We perform genetic analysis on serum levels of alanine transaminase (ALT), alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT) using data on 437,438 UK Biobank participants. Replication in 315,572 individuals from European descent from the Million Veteran Program, Rotterdam Study and Lifeline study confirms 517 liver enzyme SNPs. Read More

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Evaluation of salivary gamma-glutamyl transpeptidase as a biomarker in oral squamous cell carcinoma and precancerous lesions.

J Oral Maxillofac Pathol 2020 Sep-Dec;24(3):584. Epub 2021 Jan 9.

Department of Biochemistry, Al-Ameen Medical College and Hospital, Vijayapur, Karnataka, India.

Background: Oral cancer is such a common malignancy, but its manifestations are usually asymptomatic, and by the time the lesion is diagnosed its invasion is deep. This makes the survival rate poor and also the treatment rendered during such stages is extensive and debilitating. In this regard, a novel approach has been advocated in the estimation of biomarkers in the body fluids. Read More

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January 2021

Characterization of Ocimum basilicum synthesized silver nanoparticles and its relative toxicity to some insecticides against tobacco cutworm, Spodoptera litura Feb. (Lepidoptera; Noctuidae).

Ecotoxicol Environ Saf 2021 May 6;218:112278. Epub 2021 May 6.

Department of Botany, University of Agriculture, Faisalabad, Pakistan. Electronic address:

Tobacco cutworm, Spodoptera litura Feb. (Lepidoptera; Noctuidae) is a notorious cosmopolitan pest of numerous crops. Frequent evolution of insecticide resistance and polyphagous nature favors the survival of this voracious pest. Read More

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Georgian Med News 2021 Mar(312):31-36

I.M. Sechenov First State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russian Federation.

The goal of the study was to find ways to improve the quality of preoperative diagnosis of mucinous cystic liver neoplasms using such tumor markers as CA 19-9 and telomerase activity. We conducted a retrospective analysis and a prospective study that included the results of treatment of 80 patients with cystic neoplasm of the liver (31 with MCNL, 36 with multichamber non-parasitic cysts (NPC), 13 with stage II (WHO classification) echinococcus liver cysts. Our study showed the presence of telomerase activity in 29 (93. Read More

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Exploring boron applications in modern agriculture: A structure-activity relationship study of a novel series of multi-substitution benzoxaboroles for identification of potential fungicides.

Bioorg Med Chem Lett 2021 May 5;43:128089. Epub 2021 May 5.

Boragen, Inc., 5 Laboratory Drive, Suite 2150, Research Triangle Park, Durham, NC 27709, USA.

Several boron-containing small molecules have been approved by the US FDA to treat human diseases. We explored potential applications of boron-containing compounds in modern agriculture by pursuing multiple research and development programs. Here, we report a novel series of multi-substitution benzoxaboroles (1-36), a compound class that we recently reported as targeting geranylgeranyl transferase I (GGTase I) and thereby inhibiting protein prenylation (Kim et al. Read More

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Inoculation with Bacillus amyloliquefaciens and mycorrhiza confers tolerance to drought stress and improve seed yield and quality of soybean plant.

Physiol Plant 2021 May 8. Epub 2021 May 8.

Department of Agricultural Microbiology, Faculty of Agriculture, Mansoura University, Mansoura, Egypt.

The present study aimed to evaluate the effect of Bacillus amyloliquefaciens and/or Arbuscular Mycorrhizal Fungi (AMF) as natural biofertilizers on biomass, yield and seed nutritive quality of soybean (Giza 111). The conditions investigated include a well-watered (WW) control and irrigation withholding at the seed development stage (R5, after 90 days from sowing) (DS). Co-inoculation with B. Read More

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Phosphorylation of islet-1 serine 269 by CDK1 increases its transcriptional activity and promotes cell proliferation in gastric cancer.

Mol Med 2021 May 7;27(1):47. Epub 2021 May 7.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Beijing Key Laboratory of Protein Posttranslational Modifications and Cell Function, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education of China, Peking University, Beijing, P. R. China.

Background: Despite recent advances in diagnostic and therapeutic approaches for gastric cancer (GC), the survival of patients with advanced GC remains very low. Islet-1 (ISL1) is a LIM-homeodomain transcription factor, which is upregulated and promotes cell proliferation in GC. The exact mechanism by which ISL1 influences GC development is unclear. Read More

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Ameliorating activity of polyphenolic-rich extracts of L. leaves on pancreatic β-cell dysfunction in streptozotocin-induced diabetic rats.

J Complement Integr Med 2021 May 7. Epub 2021 May 7.

Department of Biochemistry, Phytomedicine, Natural Products, Drug and Biochemical Toxicology Group, Landmark University, Omu Aran, PMB 1001, Kwara State, Nigeria.

Objectives: To assess the ameliorative activity of polyphenolic-rich extracts of leaves on β-cell dysfunction in type-II diabetes (T2DM).

Methods: Total phenolic and flavonoid contents; α-amylase and α-glucosidase inhibitory actions and qualitative analysis of the bioactive compounds of the polyphenolic-rich extract of leaves were investigated using gas chromatography-mass spectroscopy (GC-MS). Diabetes mellitus (DM) was induced by single intraperitoneal injection of streptozotocin (60 mg/kg body weight) and the rats were orally given bound phenolic (BPE) and free phenolic extracts (FPE) of (B. Read More

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Microplastic intake and enzymatic responses in Mytilus galloprovincialis reared at the vicinities of an aquaculture station.

Chemosphere 2021 Apr 24;280:130575. Epub 2021 Apr 24.

Instituto Español de Oceanografía, Centro Oceanografico de Baleares, Muelle de Poniente S/n, 07015, Palma de Mallorca, Balearic Islands, Spain.

Aquaculture is a potential source of microplastics (MPs) that could be strong stressors for marine organisms. In this study, we evaluated the effects of MPs derived from aquaculture in antioxidant defences and oxidative stress markers in gills of Mytilus galloprovincialis. Mussels were distributed in three areas with different impacts: inside aquaculture cages, Control 1 (located inside Andratx harbour) and Control 2 (located in a no-anthropized area). Read More

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The methyltransferase domain of the Respiratory Syncytial Virus L protein catalyzes cap N7 and 2'-O-methylation.

PLoS Pathog 2021 May 6;17(5):e1009562. Epub 2021 May 6.

Aix Marseille Université, CNRS, AFMB UMR 7257, Marseille, France.

Respiratory syncytial virus (RSV) is a negative sense single-stranded RNA virus and one of the main causes of severe lower respiratory tract infections in infants and young children. RSV RNA replication/transcription and capping are ensured by the viral Large (L) protein. The L protein contains a polymerase domain associated with a polyribonucleotidyl transferase domain in its N-terminus, and a methyltransferase (MTase) domain followed by the C-terminal domain (CTD) enriched in basic amino acids at its C-terminus. Read More

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Exploring the relation between BOLD fMRI and cognitive performance using a computer-based quantitative systems pharmacology model: Applications to the COMTVAL158MET genotype and ketamine.

Eur Neuropsychopharmacol 2021 May 1;50:12-22. Epub 2021 May 1.

In Silico Biosciences, Hugo Geerts, 686 Westwind Dr, Berwyn, PA 19312, United States.

BOLD fMRI is increasingly used mostly in an observational way to probe the effect of genotypes or therapeutic intervention in normal and diseased subjects. We use a mechanism-based quantitative systems pharmacology computer model of a human cortical microcircuit, previously calibrated for the 2-back working memory paradigm, adding established biophysical principles, of glucose metabolism, oxygen consumption, neurovascular effects and the paramagnetic impact on blood oxygen levels to calculate a readout for the voxel-based BOLD fMRI signal. The objective was to study the effect of the Catechol-O-methyl Transferase Val158Met (COMT) genotype on performance and BOLD fMRI. Read More

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Allosteric activation of SARS-CoV-2 RdRp by remdesivir triphosphate and other phosphorylated nucleotides.

bioRxiv 2021 Apr 28. Epub 2021 Apr 28.

The catalytic subunit of SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), Nsp12, has a unique NiRAN domain that transfers nucleoside monophosphates to the Nsp9 protein. The NiRAN and RdRp modules form a dynamic interface distant from their catalytic sites and both activities are essential for viral replication. We report that codon-optimized (for the pause-free translation) Nsp12 exists in inactive state in which NiRAN/RdRp interactions are broken, whereas translation by slow ribosomes and incubation with accessory Nsp7/8 subunits or NTPs partially rescue RdRp activity. Read More

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Hyaluronic acid (HA)-coated naproxen-nanoparticles selectively target breast cancer stem cells through COX-independent pathways.

Mater Sci Eng C Mater Biol Appl 2021 May 10;124:112024. Epub 2021 Mar 10.

Biomaterials Group, Institute of Polymer Science and Technology (ICTP-CSIC), 28006 Madrid, Spain; Networking Biomedical Research Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain. Electronic address:

Cytotoxic chemotherapy continues to be the main therapeutic option for patients with metastatic breast cancer. Several studies have reported a significant association between chronic inflammation, carcinogenesis and the presence of cancer stem cells (CSC). We hypothesized that the use of non-steroidal anti-inflammatory drugs targeted to the CSC population could help reducing tumor progression and dissemination in otherwise hard to treat metastatic breast cancer. Read More

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Fructose Intake Impairs the Synergistic Vasomotor Manifestation of Nitric Oxide and Hydrogen Sulfide in Rat Aorta.

Int J Mol Sci 2021 Apr 30;22(9). Epub 2021 Apr 30.

Institute of Normal and Pathological Physiology, Centre of Experimental Medicine Slovak Academy of Sciences, 841 04 Bratislava, Slovakia.

In this study, we evaluated the effect of eight weeks of administration of 10% fructose solution to adult Wistar Kyoto (WKY) rats on systolic blood pressure (SBP), plasma and biometric parameters, vasoactive properties of the thoracic aorta (TA), NO synthase (NOS) activity, and the expression of enzymes producing NO and HS. Eight weeks of fructose administration did not affect SBP, glycaemia, or the plasma levels of total cholesterol or low-density and high-density lipoprotein; however, it significantly increased the plasma levels of γ-glutamyl transferase and alanine transaminase. Chronic fructose intake deteriorated endothelium-dependent vasorelaxation (EDVR) and increased the sensitivity of adrenergic receptors to noradrenaline. Read More

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Germline and somatic mutations in the pathology of pineal cyst: A whole-exome sequencing study of 93 individuals.

Mol Genet Genomic Med 2021 May 4:e1691. Epub 2021 May 4.

Vivian L. Smith Department of Neurosurgery, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX, USA.

Background: Pineal cyst is a benign lesion commonly occurring in people of any age. Until now, the underlying molecular alterations have not been explored.

Methods: We performed whole exome sequencing of 93 germline samples and 21 pineal cyst tissue samples to illustrate its genetic architecture and somatic mutations. Read More

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Cucurbituril Ameliorates Liver Damage Induced by Microcystis aeruginosa in a Mouse Model.

Front Chem 2021 14;9:660927. Epub 2021 Apr 14.

School of Engineering and Science, Jacobs University Bremen, Bremen, Germany.

is a cyanobacterium that produces a variety of cyclic heptapeptide toxins in freshwater. The protective effects of the macromolecular container cucurbit[7]uril (CB7) were evaluated using mouse models of cyanotoxin-induced liver damage. Biochemical analysis of liver function was performed to gauge the extent of liver damage after exposure to cyanobacterial crude extract [CCE; LD = 35 mg/kg body weight; intraperitoneal (i. Read More

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Hepatic Oxidative Stress, Apoptosis, and Inflammation in Broiler Chickens With Wooden Breast Myopathy.

Front Physiol 2021 14;12:659777. Epub 2021 Apr 14.

Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Joint International Research Laboratory of Animal Health and Food Safety, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, National Experimental Teaching Demonstration Center of Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Wooden breast (WB) syndrome has emerged as a global myopathy in modern commercial broiler chickens, mainly affecting the pectoralis major muscle. Recent evidence suggests that WB myopathy is a systemic disease, which might be accompanied by other physiological disparities and metabolic changes. This study was conducted to systemically investigate the potential physiological changes in liver tissues as well as the possible mechanisms involved to enhance the understanding of the etiology. Read More

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Aflatoxicosis in Pekin duckling and the effects of treatments with lycopene and silymarin.

Vet World 2021 Mar 29;14(3):788-793. Epub 2021 Mar 29.

Department of Veterinary Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Damanhour University, Egypt.

Background And Aim: Aflatoxins (AFs) are potent toxic metabolites produced from species. Whose existence in poultry ration leads to drastic economic losses, notably in duck, as the most susceptible poultry species. This study aimed to determine tissue residues of AFs, alterations in selected clinical chemistry variables in serum, mainly during the exposure period, and lycopene and silymarin's possible roles as herbal treatments against aflatoxicosis in Pekin duckling. Read More

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Microbial and functional characterization of an allochthonous consortium applied to hydrogen production from Citrus Peel Waste in batch reactor in optimized conditions.

J Environ Manage 2021 Apr 28;291:112631. Epub 2021 Apr 28.

Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo (USP), Av. Trabalhador São Carlense, 400, 13566-590, São Carlos, SP, Brazil. Electronic address:

Energy recovery from lignocellulosic waste has been studied as an alternative to the problem of inappropriate waste disposal. The present study aimed at characterizing the microbial community and the functional activity of reactors applied to H production through lignocellulosic waste fermentation in optimized conditions. The latter were identified by means of Rotational Central Composite Design (RCCD), applied to optimize allochthonous inoculum concentration (2. Read More

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Downregulating carnitine palmitoyl transferase 1 affects disease progression in the SOD1 G93A mouse model of ALS.

Commun Biol 2021 Apr 30;4(1):509. Epub 2021 Apr 30.

Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.

Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease characterized by death of motor neurons. The etiology and pathogenesis remains elusive despite decades of intensive research. Herein, we report that dysregulated metabolism plays a central role in the SOD1 G93A mouse model mimicking ALS. Read More

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