3 results match your criteria transcripts' stabilization

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Functioning of PPR Proteins in Organelle RNA Metabolism and Chloroplast Biogenesis.

Front Plant Sci 2021 9;12:627501. Epub 2021 Feb 9.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China.

The pentatricopeptide repeat (PPR) proteins constitute one of the largest nuclear-encoded protein families in higher plants, with over 400 members in most sequenced plant species. The molecular functions of these proteins and their physiological roles during plant growth and development have been widely studied. Generally, there is mounting evidence that PPR proteins are involved in the post-transcriptional regulation of chloroplast and/or mitochondrial genes, including RNA maturation, editing, intron splicing, transcripts' stabilization, and translation initiation. Read More

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February 2021

Apicidin down-regulates human papillomavirus type 16 E6 and E7 transcripts and proteins in SiHa cervical cancer cells.

Cancer Lett 2008 Dec 6;272(1):53-60. Epub 2008 Aug 6.

Poznań University of Medical Sciences, Department of Biochemistry and Molecular Biology, 6 Swiecickiego Street, 60-781 Poznań, Poland.

Virtually all cervical cancer morbidities are associated with genital skin or mucosa cell infection with human papillomavirus (HPV). The HPV oncogenic proteins E6 and E7 are able to inactivate p53 and Rb proteins, which results in malignant transformation. Employing quantitative real-time PCR and Western blot analysis, we observed that apicidin histone deacetylase (HDAC) inhibitor significantly reduced HPV16-E6 and -E7 transcripts and protein levels in SiHa cervical cancer cells. Read More

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December 2008

Specific sequence elements in the 5' untranslated regions of rbcL and atpB gene mRNas stabilize transcripts in the chloroplast of Chlamydomonas reinhardtii.

RNA 2001 Jul;7(7):1024-33

Department of Biology, University of Oslo, Blindern, Norway.

Using a series of point mutations in chimeric reporter gene constructs consisting of the 5' regions of the Chlamydomonas chloroplast rbcL or atpB genes fused 5' to the coding sequence of the bacterial uidA (GUS) gene, RNA-stabilizing sequence elements were identified in vivo in the 5' untranslated regions (5' UTRs) of transcripts of the chloroplast genes rbcL and atpB in Chlamydomonas reinhardtii. In chimeric rbcL 5' UTR:GUS transcripts, replacement of single nucleotides in the 10-nt sequence 5'-AUUUCCGGAC-3', extending from positions +38 to +47 relative to the transcripts' 5' terminus, shortened transcript longevity and led to a reduction in transcript abundance of more than 95%. A similar mutational analysis of atpB 5' UTR:GUS transcripts showed that the 12-nt atpB 5' UTR sequence 5'-AUAAGCGUUAGU-3', extending from position +31 to position +42, is important for transcript stability and transcript accumulation in the chloroplast of Chlamydomonas. Read More

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